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Search Results (1,269)

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22 pages, 775 KiB  
Review
Bioactive Compounds, Technological Advances, and Sustainable Applications of Avocado (Persea americana Mill.): A Critical Review
by Amanda Priscila Silva Nascimento, Maria Elita Martins Duarte, Ana Paula Trindade Rocha and Ana Novo Barros
Foods 2025, 14(15), 2746; https://doi.org/10.3390/foods14152746 - 6 Aug 2025
Abstract
Avocado (Persea americana), originally from Mesoamerica, has emerged as a focus of intense scientific and industrial interest due to its unique combination of nutritional richness, bioactive potential, and technological versatility. Its pulp, widely consumed across the globe, is notably abundant in [...] Read more.
Avocado (Persea americana), originally from Mesoamerica, has emerged as a focus of intense scientific and industrial interest due to its unique combination of nutritional richness, bioactive potential, and technological versatility. Its pulp, widely consumed across the globe, is notably abundant in monounsaturated fatty acids, especially oleic acid, which can comprise over two-thirds of its lipid content. In addition, it provides significant levels of dietary fiber, fat-soluble vitamins such as A, D, E and K, carotenoids, tocopherols, and phytosterols like β-sitosterol. These constituents are consistently associated with antioxidant, anti-inflammatory, glycemic regulatory, and cardioprotective effects, supported by a growing body of experimental and clinical evidence. This review offers a comprehensive and critical synthesis of the chemical composition and functional properties of avocado, with particular emphasis on its lipid profile, phenolic compounds, and phytosterols. It also explores recent advances in environmentally sustainable extraction techniques, including ultrasound-assisted and microwave-assisted processes, as well as the application of natural deep eutectic solvents. These technologies have demonstrated improved efficiency in recovering bioactives while aligning with the principles of green chemistry. The use of avocado-derived ingredients in nanostructured delivery systems and their incorporation into functional foods, cosmetics, and health-promoting formulations is discussed in detail. Additionally, the potential of native cultivars and the application of precision nutrition strategies are identified as promising avenues for future innovation. Taken together, the findings underscore the avocado’s relevance as a high-value matrix for sustainable development. Future research should focus on optimizing extraction protocols, clarifying pharmacokinetic behavior, and ensuring long-term safety in diverse applications. Full article
(This article belongs to the Special Issue Feature Review on Nutraceuticals, Functional Foods, and Novel Foods)
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19 pages, 1102 KiB  
Article
Assessing the Adoption and Feasibility of Green Wall Systems in Construction Projects in Nigeria
by Oluwayinka Seun Oke, John Ogbeleakhu Aliu, Damilola Ekundayo, Ayodeji Emmanuel Oke and Nwabueze Kingsley Chukwuma
Sustainability 2025, 17(15), 7126; https://doi.org/10.3390/su17157126 - 6 Aug 2025
Abstract
This study aims to evaluate the level of awareness and practical adoption of green wall systems in the Nigerian construction industry. It seeks to examine the current state of green wall implementation and recommend strategies to enhance their integration into construction practices among [...] Read more.
This study aims to evaluate the level of awareness and practical adoption of green wall systems in the Nigerian construction industry. It seeks to examine the current state of green wall implementation and recommend strategies to enhance their integration into construction practices among Nigerian construction professionals. A thorough review of the existing literature was conducted to identify different types of green wall systems. Insights from this review informed the design of a structured questionnaire, which was distributed to construction professionals based in Lagos State. The data collected were analyzed using statistical tests. The study reveals that while there is generally high awareness of green wall systems among Nigerian construction professionals, the practical use remains low, with just 8 out of the 18 systems being actively implemented, eclipsing the mean value of 3.0. The findings underscore the need for targeted education, industry incentives, and increased advocacy to encourage the use of green wall systems in the Nigerian construction sector. The results have significant implications for the Nigerian construction industry. The limited awareness and adoption of green wall systems highlight the need for strategic actions from policymakers, industry leaders and educational institutions. Promoting the use of green walls could drive more sustainable building practices, improve environmental outcomes and support the broader goals of decarbonization and circularity in construction. This research adds to the body of knowledge on sustainable construction by offering a detailed evaluation of green wall awareness and adoption within the Nigerian context. While green wall systems have been studied globally, this research provides a regional perspective, which in this case focuses on Lagos State. The study’s recognition of the gap between awareness and implementation highlights an important area for future research and industry development. Full article
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19 pages, 1869 KiB  
Article
Optimization of Stresses near Reinforced Holes in Relation to Sustainable Design of Composite Structural Elements
by Bartosz Miller, Marta Maksymovych, Olesia Maksymovych and Fedir Gagauz
Sustainability 2025, 17(15), 7103; https://doi.org/10.3390/su17157103 - 5 Aug 2025
Abstract
A method for selecting mechanical properties and geometry of reinforcing overlays to increase the strength of composite structural elements with holes has been developed. The method is based on the developed algorithm for calculating stress concentration near holes reinforced with inserted rings or [...] Read more.
A method for selecting mechanical properties and geometry of reinforcing overlays to increase the strength of composite structural elements with holes has been developed. The method is based on the developed algorithm for calculating stress concentration near holes reinforced with inserted rings or glued composite reinforcing overlays. The determination of stresses near holes and overlays is reduced to solving a system of singular integral equations. The kernels of these equations are constructed using Green’s solution, which allows a reduction in the number of equations to four. It is shown that the stress concentration near holes can be significantly reduced by optimizing the thickness, elastic properties, and shape of the overlays. The stress calculations performed based on the three-dimensional theory of elasticity confirmed the reliability of the results obtained within the framework of the plane problem of an anisotropic body. The results obtained, in accordance with the concept of sustainable development, enable the develop simple methods for increasing reliability, reducing material consumption, and reducing the manufacturing and operating costs of composite structures in the aerospace and mechanical engineering industries. Full article
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23 pages, 8079 KiB  
Article
Electrophoretic Deposition of Green-Synthesized Hydroxyapatite on Thermally Oxidized Titanium: Enhanced Bioactivity and Antibacterial Performance
by Mariana Relva, Daniela Santo, Ricardo Alexandre, Pedro Faia, Sandra Carvalho, Zohra Benzarti and Susana Devesa
Appl. Sci. 2025, 15(15), 8598; https://doi.org/10.3390/app15158598 (registering DOI) - 2 Aug 2025
Viewed by 127
Abstract
Titanium alloys such as Ti-6Al-4V are widely used in biomedical implants due to their excellent mechanical properties and biocompatibility, but their bioinert nature limits osseointegration and antibacterial performance. This study proposes a multifunctional surface coating system integrating a thermally oxidized TiO2 interlayer [...] Read more.
Titanium alloys such as Ti-6Al-4V are widely used in biomedical implants due to their excellent mechanical properties and biocompatibility, but their bioinert nature limits osseointegration and antibacterial performance. This study proposes a multifunctional surface coating system integrating a thermally oxidized TiO2 interlayer with a hydroxyapatite (HAp) top layer synthesized via a green route using Hylocereus undatus extract. The HAp was deposited by electrophoretic deposition (EPD), enabling continuous coverage and strong adhesion to the pre-treated Ti-6Al-4V substrate. Structural, morphological, chemical, and electrical characterizations were performed using XRD, SEM, EDS, Raman spectroscopy, and impedance spectroscopy. Bioactivity was assessed through apatite formation in simulated body fluid (SBF), while antibacterial properties were evaluated against Staphylococcus aureus. The results demonstrated successful formation of crystalline TiO2 (rutile phase) and calcium-rich HAp with good surface coverage. The HAp-coated surfaces exhibited significantly enhanced bioactivity and strong antibacterial performance, likely due to the combined effects of surface roughness and the bioactive compounds present in the plant extract. This study highlights the potential of eco-friendly, bio-inspired surface engineering to improve the biological performance of titanium-based implants. Full article
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18 pages, 1467 KiB  
Article
Effects of a 16-Week Green Exercise Program on Body Composition, Sleep, and Nature Connection in Postmenopausal Women
by Helena Moreira, Chiara Tuccella, Emília Alves, Andreia Teixeira, Carlos Moreira, Irene Oliveira, Valerio Bonavolontà and Catarina Abrantes
Int. J. Environ. Res. Public Health 2025, 22(8), 1216; https://doi.org/10.3390/ijerph22081216 - 1 Aug 2025
Viewed by 149
Abstract
Physical activity, particularly when practiced in natural settings, has well-established benefits for overall health, sleep, and body composition. These effects are especially important for postmenopausal women, although research specifically targeting this population remains limited. The study evaluated a 16-week multicomponent outdoor exercise program [...] Read more.
Physical activity, particularly when practiced in natural settings, has well-established benefits for overall health, sleep, and body composition. These effects are especially important for postmenopausal women, although research specifically targeting this population remains limited. The study evaluated a 16-week multicomponent outdoor exercise program (cardiorespiratory, strength, balance, coordination, and flexibility training) in postmenopausal women, consisting of three 60 min sessions per week. Participants were non-randomly assigned to an experimental group (EG, n = 55) and a control group (CG, n = 20). Measurements were taken at baseline and after 16 weeks, including body composition, sleep (duration and quality), and connection with nature. No significant differences were observed between groups at baseline. After the intervention, the EG and CG presented significant differences (p ≤ 0.01) in the rates of change in body mass, fat mass (FM; −9.26% and −1.21%, respectively), and visceral fat level (VFL; −13.46 points and −3.80 points). These differences were also observed for the sleep fragmentation index (p ≤ 0.01), but not for connection with nature. A significant interaction effect (p < 0.01) of time × group was observed for %FM, VFL, and appendicular skeletal muscle mass. Exercise duration had an effect (p = 0.043) on participants’ personal and affective identification with nature, and the time × group × medication interaction significantly influenced sleep efficiency (p = 0.034). The exercise program proved effective in reducing total and central adiposity levels; however, it did not lead to improvements in sleep duration, sleep quality, or connection with nature. Full article
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20 pages, 998 KiB  
Article
Colony Nutrition Enhances Bee Resilience to Fungicides, While the Benefit of Propolis Supplementation Depends on Stress Conditions
by Yara Martins Molina Ferraz, Aline Yukari Kato, Tainá Angelica de Lima Freitas, Cássia Regina de Avelar Gomes, Thais Regina Ramos Alves, Matheus Franco Trivellato, Samir Moura Kadri, Ricardo de Oliveira Orsi, David De Jong, Jaqueline Dalbello Biller and Daniel Nicodemo
Agriculture 2025, 15(15), 1665; https://doi.org/10.3390/agriculture15151665 - 1 Aug 2025
Viewed by 215
Abstract
Enhanced colony nutrition can support brood development, resulting in better physiological conditions and increased resilience in adult honey bees, particularly under stress. This study investigated the effects of colony nutrition and adult dietary supplementation with green propolis on bee health under fungicide exposure. [...] Read more.
Enhanced colony nutrition can support brood development, resulting in better physiological conditions and increased resilience in adult honey bees, particularly under stress. This study investigated the effects of colony nutrition and adult dietary supplementation with green propolis on bee health under fungicide exposure. Colonies were managed under food restriction or nutritional supplementation for 22 weeks. Newly emerged bees from each colony were then caged and fed protein diets consisting of honey-pollen patties contaminated or not with fungicide, and sucrose sugar syrup with or without aqueous green propolis extract. Bees from supplemented colonies showed greater body weight, higher hemolymph protein levels, and higher consumption of protein food after seven days in cages. Fungicide exposure reduced hemolymph protein levels, altered the expression of detoxification and immune-related genes, and significantly decreased bee survival. Interestingly, propolis supplementation alone changed gene expression patterns and slightly reduced longevity compared to bees not exposed to propolis or fungicide. However, under fungicide stress, bees that ingested propolis survived longer, indicating a protective effect. While colony nutritional supplementation clearly promotes honey bee resilience against fungicide exposure, feeding propolis also showed promising effects, though further studies are needed to determine an optimal dietary concentration. Full article
(This article belongs to the Special Issue Honey Bees and Wild Pollinators in Agricultural Ecosystems)
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15 pages, 953 KiB  
Review
Influence of Matcha and Tea Catechins on the Progression of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)—A Review of Patient Trials and Animal Studies
by Danuta I. Kosik-Bogacka and Katarzyna Piotrowska
Nutrients 2025, 17(15), 2532; https://doi.org/10.3390/nu17152532 - 31 Jul 2025
Viewed by 427
Abstract
Metabolic dysfunction-associated fatty liver disease (MASLD) is a chronic, non-communicable spectrum of diseases characterized by lipid accumulation. It is often asymptomatic, and its prevalence varies by region, age, gender, and economic status. It is estimated that 25% of the world’s population currently suffer [...] Read more.
Metabolic dysfunction-associated fatty liver disease (MASLD) is a chronic, non-communicable spectrum of diseases characterized by lipid accumulation. It is often asymptomatic, and its prevalence varies by region, age, gender, and economic status. It is estimated that 25% of the world’s population currently suffer from MAFLD, and 20 million patients will die from MAFLD-related diseases. In the last 20 years, tea and anti-obesity research have indicated that regularly consuming tea decreases the risk of cardiovascular disease, stroke, obesity, diabetes, and metabolic syndrome (MeS). In this review, we aimed to present studies concerning the influence of matcha extracts and epigallocatechin-3 gallate (EGCG) supplements on metabolic functions in the context of MAFLD in human and animal studies. The published data show promise. In both human and animal studies, the beneficial effects on body weight, cholesterol levels, and liver metabolism and function were noted, even in short-period experiments. The safety levels for EGCG and green tea extract consumption are marked. More experiments are needed to confirm the results observed in animal studies and to show the mechanisms by which green tea exerts its effects. The preliminary data from research concerning microbiota or epigenetic changes observed after polyphenols and green tea consumption need to be expanded. To improve the efficiency and availability of green tea or supplement consumption as a treatment for MAFLD patients, more research with larger groups and longer study durations is needed. Full article
(This article belongs to the Special Issue Phytonutrients in Diseases of Affluence)
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36 pages, 9312 KiB  
Review
Current Progress in the Biosynthesis of Metal Sulfide Nanomaterials for the Degradation of Dyes: A Review
by Carol D. Langa, Nonhlangabezo Mabuba and Nomso C. Hintsho-Mbita
Catalysts 2025, 15(8), 727; https://doi.org/10.3390/catal15080727 - 30 Jul 2025
Viewed by 284
Abstract
The contamination of water bodies by industrial dyes poses a significant environmental challenge on a global scale. Conventional wastewater treatment methods often suffer from limitations related to high cost, limited efficiency, and potential secondary environmental impacts. Recent advances in photocatalytic technologies have highlighted [...] Read more.
The contamination of water bodies by industrial dyes poses a significant environmental challenge on a global scale. Conventional wastewater treatment methods often suffer from limitations related to high cost, limited efficiency, and potential secondary environmental impacts. Recent advances in photocatalytic technologies have highlighted the potential of metal sulfide-based photocatalysts, particularly those synthesized through environmentally friendly, plant-mediated approaches, as promising alternatives for efficient and sustainable dye degradation. However, despite their promising potential, metal sulfide photocatalysts often suffer from limitations such as photocorrosion, low stability under irradiation, and rapid recombination of charge carriers, which restrict their long-term applicability. In light of these challenges, this review provides a comprehensive examination of the physicochemical characteristics, synthetic strategies, and photocatalytic applications of metal sulfides. Particular emphasis is placed on green synthesis routes employing plant-derived extracts, which offer environmentally benign and sustainable alternatives to conventional methods. Moreover, the review elucidates various modification approaches, most notably, the formation of heterostructures, as viable strategies to enhance photocatalytic efficiency and mitigate the aforementioned drawbacks. The green synthesis of metal sulfides, aligned with the principles of green chemistry, offers a promising route toward the development of sustainable and environmentally friendly water treatment technologies. Full article
(This article belongs to the Special Issue Recent Advances in Photocatalysis for Environmental Applications)
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15 pages, 1125 KiB  
Article
Mixed Green Banana (Musa spp.) Pulp and Peel Flour Reduced Body Weight Gain and Adiposity and Improved Lipid Profile and Intestinal Morphology in Wistar Rats
by Leonara Martins Viana, Bárbara Pereira da Silva, Fabiana Silva Rocha Rodrigues, Laise Trindade Paes, Marcella Duarte Villas Mishima, Renata Celi Lopes Toledo, Elad Tako, Hércia Stampini Duarte Martino and Frederico Barros
Nutrients 2025, 17(15), 2493; https://doi.org/10.3390/nu17152493 - 30 Jul 2025
Viewed by 251
Abstract
Background and Objectives: In recent years, there has been growing interest in the production of ingredients rich in dietary fiber and antioxidants, such as green banana flours. This study evaluated the effect of consumption of mixed green banana pulp (PF) and peel (PeF) [...] Read more.
Background and Objectives: In recent years, there has been growing interest in the production of ingredients rich in dietary fiber and antioxidants, such as green banana flours. This study evaluated the effect of consumption of mixed green banana pulp (PF) and peel (PeF) flours on the body weight gain, adiposity, lipid profile, and intestinal morphology of Wistar rats. Methods: Male young rats were divided into four groups (n = 8) that received a standard diet (SD), or one of the following three test diets: M1 (SD + 90% PF/10% PeF), M2 (SD + 80% PF/20% PeF), or P (SD + 100% PF) for 28 days. Results: Rats from M1, M2, and P groups showed reduced body weight gain and adiposity and had lower contents of total cholesterol, LDL-c, VLDL-c, and triglycerides. Animals from M1 and M2 groups had an increase in cecum weight, fecal moisture, acetic acid concentration, and crypt depth and reduced fecal pH. Moreover, consumption of the M1, M2, and P diets increased the expression of proteins involved in intestinal functionality. Significant negative correlations were observed between consumption of resistant starch and soluble dietary fiber, from the flours, and weight gain (r = −0.538 and r = −0.538, respectively), body adiposity (r = −0.780 and r = −0.767, respectively), total cholesterol (r = −0.789 and r = −0.800, respectively), and triglycerides (r = −0.790 and r = −0.786, respectively). Conclusions: Mixed green banana pulp and peel flour proved to be a viable alternative as a food ingredient that can promote weight loss, improve lipid profile and intestinal morphology, and minimize post-harvest losses. Full article
(This article belongs to the Section Nutrition and Obesity)
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17 pages, 7288 KiB  
Article
Non-Linear Prediction Model for the Strength of Medium-to-Low-Grade Phosphate Tailings Cemented Backfill
by Weizhong Zhang, Menglai Wang, Shujian Li, Yuandi Xia and Qinrong Kang
Appl. Sci. 2025, 15(15), 8358; https://doi.org/10.3390/app15158358 - 27 Jul 2025
Viewed by 264
Abstract
Developing green mining technology for medium-to low-grade mines requires achieving minimal or no damage to the mining area’s ecological environment. A medium-to low-grade phosphate mine in Hubei Province was taken as the research object in this study. The tailings were selected as the [...] Read more.
Developing green mining technology for medium-to low-grade mines requires achieving minimal or no damage to the mining area’s ecological environment. A medium-to low-grade phosphate mine in Hubei Province was taken as the research object in this study. The tailings were selected as the main filling aggregate. Indoor tests and theoretical analysis were conducted to analyze the influence of curing age, the water–cement ratio, the cement–sand ratio, and slurry concentration on the strength of the cemented backfill. Furthermore, a multi-factor non-linear mathematical model of the strength of the cementitious filler was established. The study results indicated that the strength of backfill increased linearly with the increase in the curing age, decreased negatively with the increase in the water–cement ratio, and increased exponentially with the increase in the cement–sand ratio and the slurry concentration. The multivariate non-linear prediction model of the strength of the filling body at different ages was also established based on the test results. This predictive model could effectively predict the strength of the cemented backfill, and the error value was not larger than 4%. Our research results can lay a theoretical foundation for developing medium-to low-grade phosphate mine filling with tailings as the main filling aggregate. Full article
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10 pages, 2396 KiB  
Communication
Preparation of Permeable Porous Alumina Ceramics by Gel Casting Combined with Particle Stacking and Sintering Method
by Zhe Cheng, Yuanqing Chen, Zhenping Wu and Yang Liu
Materials 2025, 18(15), 3463; https://doi.org/10.3390/ma18153463 - 24 Jul 2025
Viewed by 277
Abstract
Porous ceramics have been widely used in various fields. In this paper, porous ceramics with through-hole structures were prepared using a novel and eco-friendly gel casting method with carrageenan as the gelling agent. Especially, the idea of large size particle stacking is introduced [...] Read more.
Porous ceramics have been widely used in various fields. In this paper, porous ceramics with through-hole structures were prepared using a novel and eco-friendly gel casting method with carrageenan as the gelling agent. Especially, the idea of large size particle stacking is introduced into the gel casting process. By introducing large size alumina aggregates as raw materials, and small size micropowders as filling materials, micropores were directly formed after the green body was sintered. To tune the pore size, pore structure, gas permeability, the strength of the final porous ceramics, the components of the raw materials including the alumina aggregates, the filling materials, and sintering additives in the slurry were precisely designed. Porous Al2O3-based ceramics with high gas permeability, high flexural strength, and moderate porosity were finally obtained. Full article
(This article belongs to the Section Advanced and Functional Ceramics and Glasses)
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23 pages, 2594 KiB  
Article
Formation and Characterization of Xylitol-Modified Glycidyl Methacrylate-co-Ethyl Methacrylate Matrices for Controlled Release of Antimicrobial Compounds
by Adam Chyzy, Przemysław Gnatowski, Edyta Piłat, Maciej Sienkiewicz, Katarzyna Wozniak, Marta Wojnicka, Krzysztof Brzezinski and Marta E. Plonska-Brzezinska
Molecules 2025, 30(15), 3083; https://doi.org/10.3390/molecules30153083 - 23 Jul 2025
Viewed by 195
Abstract
Wounds are undeniably important gateways for pathogens to enter the body. In addition to their detrimental local effects, they can also cause adverse systemic effects. For this reason, developing methods for eradicating pathogens from wounds is a challenging medical issue. Polymers, particularly hydrogels, [...] Read more.
Wounds are undeniably important gateways for pathogens to enter the body. In addition to their detrimental local effects, they can also cause adverse systemic effects. For this reason, developing methods for eradicating pathogens from wounds is a challenging medical issue. Polymers, particularly hydrogels, are one of the more essential materials for designing novel drug-delivery systems, thanks to the ease of tuning their structures. This work exploits this property by utilizing copolymerization, microwave modification, and drug-loading processes to obtain antibacterial gels. Synthesized xylitol-modified glycidyl methacrylate-co-ethyl methacrylate ([P(EMA)-co-(GMA)]-Xyl]) matrices were loaded with bacitracin, gentian violet, furazidine, and brilliant green, used as active pharmaceutical ingredients (APIs). The hydrophilic properties, API release mechanism, and antibacterial properties of the obtained hydrogels against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus epidermidis containing [P(EMA)-co-(GMA)]-Xyl] were studied. The hydrogels with the APIs efficiently inhibit bacteria growth with low doses of drugs, and our findings are statistically significant, confirmed with ANOVA analysis at p = 0.05. The results confirmed that the proposed system is hydrophilic and has extended the drug-release capabilities of APIs with a controlled burst effect based on [P(EMA)-co-(GMA)]-Xyl] content in the hydrogel. Hydrogels are characterized by the prolonged release of APIs in a very short time (a few minutes). Although the amount of released APIs is about 10%, it still exceeds the minimum inhibitory concentrations of drugs. Several kinetic models (first-order, second-order, Baker–Lonsdale, and Korsmeyer–Peppas) were applied to fit the API release data from the [P(EMA)-co-(GMA)]-Xyl-based hydrogel. The best fit of the Korsmeyer–Peppas kinetic model to the experimental data was determined, and it was confirmed that a diffusion-controlled release mechanism of the APIs from the studied hydrogels is dominant, which is desirable for applications requiring a consistent, controlled release of therapeutic agents. A statistical analysis of API release using Linear Mixed Model was performed, examining the relationship between % mass of API, sample (hydrogels and control), time, sample–time interaction, and variability between individuals. The model fits the data well, as evidenced by the determination coefficients close to 1. The analyzed interactions in the data are reliable and statistically significant (p < 0.001). The outcome of this study suggests that the presented acrylate-based gel is a promising candidate for developing wound dressings. Full article
(This article belongs to the Special Issue Advances in Functional Polymers and Their Applications)
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15 pages, 3612 KiB  
Article
Postmortem Changes in mRNA Expression and Tissue Morphology in Brain and Femoral Muscle Tissues of Rat
by Sujin Choi, Minju Jung, Mingyoung Jeong, Sohyeong Kim, Dong Geon Lee, Kwangmin Park, Xianglan Xuan, Heechul Park, Dong Hyeok Kim, Jungho Kim, Min Ho Lee, Yoonjung Cho and Sunghyun Kim
Int. J. Mol. Sci. 2025, 26(15), 7059; https://doi.org/10.3390/ijms26157059 - 22 Jul 2025
Viewed by 200
Abstract
The postmortem interval (PMI), defined as the time elapsed between death and the discovery or examination of the body, is a crucial parameter in forensic science for estimating the time of death. There are many ways to measure the PMI, such as Henssge’s [...] Read more.
The postmortem interval (PMI), defined as the time elapsed between death and the discovery or examination of the body, is a crucial parameter in forensic science for estimating the time of death. There are many ways to measure the PMI, such as Henssge’s nomogram, which uses rectal temperature measurement; livor mortis; rigor mortis; and forensic entomology. However, these methods are usually affected by various conditions in the surrounding environment. The purpose of the present study was to compare molecular genetics and histological changes in the brain and skeletal muscle tissues of SD rats over increasing periods of time after death. For the PMIs, we considered 0 h, 6 h, 12 h, 24 h, 36 h, 48 h, 4 days, 6 days, 8 days, 10 days, 14 days, and 21 days and compared them at 4 °C and 26 °C. Hematoxylin and Eosin (H&E) staining was performed to observe tissue changes. Morphological tissue changes were observed in cells for up to 21 days at 4 °C, and cell destruction was visually confirmed after 14 days at 26 °C. Total RNA (tRNA) was isolated from each tissue sample, and complementary DNA (cDNA) was synthesized. A reverse transcription quantitative PCR (RT-qPCR) SYBR Green assay targeting three types of housekeeping genes, including Gapdh, Sort1, B2m, and 5S rRNA, was performed. The results showed that Gapdh and 5S rRNA were highly stable and could be better RNA targets for estimating the PMI in brain and skeletal muscle tissues. Conversely, Sort1 and B2m showed poor stability and low expression levels. In conclusion, these molecular biomarkers could be used as auxiliary indicators of the PMI in human, depending on the stability of the marker. Full article
(This article belongs to the Special Issue Advances in Molecular Forensic Pathology and Toxicology: An Update)
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7 pages, 395 KiB  
Proceeding Paper
Health Effects of Coffee Products on Oxidative Stress-Related Metabolic Disorders: An Updated Perspective
by Santa Anabel Hernández-Abreu and Francisco Javier Álvarez-Martínez
Proceedings 2025, 119(1), 9; https://doi.org/10.3390/proceedings2025119009 - 18 Jul 2025
Viewed by 526
Abstract
Coffee, rich in polyphenols, has been studied for its impact on obesity and oxidative stress. Its bioactive compounds combat oxidative stress, which is linked to chronic diseases. This study provides a comprehensive, artificial intelligence-enhanced review of the scientific literature, highlighting the impact of [...] Read more.
Coffee, rich in polyphenols, has been studied for its impact on obesity and oxidative stress. Its bioactive compounds combat oxidative stress, which is linked to chronic diseases. This study provides a comprehensive, artificial intelligence-enhanced review of the scientific literature, highlighting the impact of coffee and its derivatives on these disorders. Several studies show that coffee husks and green coffee supplements reduce body weight and inflammation by increasing antioxidant defenses. In conclusion, coffee and its derived products, including valorized by-products, represent a promising avenue for dietary strategies aimed at preventing and managing oxidative stress-associated metabolic disorders and promoting overall metabolic health. Full article
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16 pages, 1942 KiB  
Article
Genetic, Diversity, and Muscle Quality Among Red and Green Color Morphs of Asian Swimming Crab (Charybdis japonica): Implications for Accurate Species Recognition and Sustainable Management
by Bingqian Zhang, Yuhang He, Maninder Meenu, Ying Liu and Yusheng Jiang
Foods 2025, 14(14), 2516; https://doi.org/10.3390/foods14142516 - 18 Jul 2025
Viewed by 357
Abstract
In this study, two color morphs (red and green) of Asian swimming crab (Charybdis japonica) commonly distributed in the China Sea area were analyzed for their L*a*b* values, carapace and inner membrane histology, morphological characteristics, mitochondrial DNA sequences, muscle texture, and [...] Read more.
In this study, two color morphs (red and green) of Asian swimming crab (Charybdis japonica) commonly distributed in the China Sea area were analyzed for their L*a*b* values, carapace and inner membrane histology, morphological characteristics, mitochondrial DNA sequences, muscle texture, and amino acid composition. The results showed that compared with the green morph group, the red morph group exhibits higher aggregation of melanocytes and fewer pigment cells in the inner membrane. In addition, L* and b* of the carapace, and L* values of the inner membrane were lower in red morph group. Both populations of C. japonica also exhibit significant differences in their morphological parameters, including carapace length, body weight, and pincer width. However, the coefficient of variation for these morphological parameters did not correspond to the subspecies level. The mitochondrial DNA analysis also revealed sequence identity of COI (98.96%) and ITS-1 (99.71%) genes in both groups, supporting them to belong to the same species. Both groups also presented significant differences in their muscle texture characteristics, including adhesiveness, springiness, and gumminess, but no significant differences were observed in the muscle amino acid composition. Overall, red and green morphs of C. japonica show differences in their body color, morphological characteristics, and muscle quality, but still belong to the same species. Full article
(This article belongs to the Section Foods of Marine Origin)
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