Search Results (375)

Implementing nature-based solutions (NbSs) for wildfire risk management and other hazards has been challenging in emerging economies due to the high costs, the lack of immediate returns on investment, and stringent inclusion criteria set by organizations like the IUCN and domain experts. To address these challenges, this exploratory study proposes a new concept: green/blue initiatives. These initiatives represent intermediate steps, encompassing small-scale, community-driven activities that can evolve into recognized NbSs over time. To explore this concept, experiences related to wildfire prevention in the Biobío region of Chile were analyzed through primary and secondary source reviews. The analysis identified three initiatives qualifying as green/blue initiatives: (1) goat grazing in Santa Juana to reduce fuel loads, (2) a restoration prevention farm model in Florida called Faro de Restauración Mahuidanche and (3) the Conservation Landscape Strategy in Nonguén. They were examined in detail using data collected from site visits and interviews. In contrast to Chile’s prevailing wildfire policies, which focus on costly, large-scale fire suppression efforts, these initiatives emphasize the importance of reframing wildfire as a manageable ecological process. Lastly, the challenges and enabling factors for adopting green/blue initiatives are discussed, highlighting their potential to pave the way for future NbS implementation in central Chile. Full article

Water infiltration into soil is a key process in regulating the hydrological cycle and sustaining ecosystem services in high-Andean environments. However, limited information is available regarding its dynamics in these ecosystems. This study evaluated the influence of three types of vegetation cover and soil properties on water infiltration in a high-Andean environment. A double-ring infiltrometer, the Water Drop Penetration Time (WDPT, s) method, and laboratory physicochemical characterization were employed. Soils under forest cover exhibited significantly higher quasi-steady infiltration rates (i_s, 0.248 ± 0.028 cm·min⁻¹) compared to grazing areas (0.051 ± 0.016 cm·min⁻¹) and agricultural lands (0.032 ± 0.013 cm·min⁻¹). Soil organic matter content was positively correlated with i_s. The modified Kostiakov infiltration model provided the best overall fit, while the Horton model better described infiltration rates approaching i_s. Sand and clay fractions, along with K⁺, Ca²⁺, and Mg²⁺, were particularly significant during the soil’s wet stages. In drier stages, increased Na⁺ concentrations and decreased silt content were associated with higher water repellency. Based on WDPT, agricultural soils exhibited persistent hydrophilic behavior even after drying (median [IQR] from 0.61 [0.38] s to 1.24 [0.46] s), whereas forest (from 2.84 [3.73] s to 3.53 [24.17] s) and grazing soils (from 4.37 [1.95] s to 19.83 [109.33] s) transitioned to weakly or moderately hydrophobic patterns. These findings demonstrate that native Andean forest soils exhibit a higher infiltration capacity than soils under anthropogenic management (agriculture and grazing), highlighting the need to conserve and restore native vegetation cover to strengthen water resilience and mitigate the impacts of land-use change. Full article

We investigated the dispersal ability of Euphydryas aurinia provincialis in a local-scale analysis within a single habitat patch of the Colfiorito highlands metapopulation. Our findings indicate that inside a single node, the organization of nesting patches can be conceptualized as a metapopulation itself, where reproductive sites, despite their spatial proximity, can act as either source or sink habitats depending on environmental conditions. We conducted fieldwork in six nesting patches inside a single node, capturing, marking, and recapturing individuals to assess their spatial distribution and movement tendencies at a large landscape scale. We found a high degree of site fidelity among individuals, with many recaptures occurring within the original marking site, but also a sex-based difference in movement patterns; females dispersed farther than males, likely driven by reproductive strategies, while males remained more localized, prioritizing mate-searching. Our findings suggest a complex dynamic in habitat connectivity: pastures and abandoned fields, despite being open, seem to act like sink areas, while breeding sites with shrub and tree cover act as source habitats, offering optimal conditions for reproduction. Individuals, especially females, from these source areas were later compelled to disperse into open habitats, highlighting a nuanced interaction between landscape structure and population dynamics. These results highlight the importance of maintaining habitat corridors to support metapopulation dynamics and prevent genetic isolation; the abandonment of traditional grazing practices is leading to the rapid closure of these source habitats, posing a severe risk of local extinction. Conservation efforts should prioritize the preservation of these source habitats to ensure the long-term viability of E. a. provincialis populations in fragmented landscapes. Full article

Grazing affects soil organic carbon (SOC) through plant removal, livestock trampling, and manure deposition. However, the impact of grazing on SOC is also influenced by multiple factors such as climate, soil properties, and management approaches. Despite extensive research, the mechanisms by which grazing intensity influences SOC density in grasslands remain incompletely understood. This study examines the effects of varying grazing intensities on SOC density (0–30 cm) dynamics in temperate grasslands of northern China using field surveys and experimental analyses in a typical steppe ecosystem of Inner Mongolia. Results show that moderate grazing (3.8 sheep units/ha/yr) led to substantial consumption of aboveground plant biomass. Relative to the ungrazed control (0 sheep units/ha/yr), aboveground plant biomass was reduced by 40.5%, 36.2%, and 50.6% in the years 2016, 2019, and 2020, respectively. Compensatory growth failed to fully offset biomass loss, and there were significant reductions in vegetation carbon storage and cover (p < 0.05). Reduced vegetation cover increased bare soil exposure and accelerated topsoil drying and erosion. This degradation promoted the downward migration of SOC from surface layers. Quantitative analysis revealed that moderate grazing significantly reduced surface soil (0–10 cm) organic carbon density by 13.4% compared to the ungrazed control while significantly increasing SOC density in the subsurface layer (10–30 cm). Increased precipitation could mitigate the SOC transfer and enhance overall SOC accumulation. However, it might negatively affect certain labile SOC fractions. Elucidating the mechanisms of SOC variation under different grazing intensities and precipitation regimes in semi-arid grasslands could improve our understanding of carbon dynamics in response to environmental stressors. These insights will aid in predicting how grazing systems influence grassland carbon cycling under global climate change. Full article

Semi-natural grasslands are some of the most species-rich habitats in Europe and provide important ecosystem services such as biodiversity conservation, carbon sequestration and soil fertility maintenance. This study investigates how different intensities of grassland management affect the composition of functional groups and soil chemical properties. Five grassland management systems were analyzed: Cut3—three cuts per year; LGI—low grazing intensity; CG—combined cutting and grazing; Cut4—four cuts per year; and HGI—high grazing intensity. The functional groups assessed were grasses, legumes and forbs, while soil samples from three depths (0–10, 10–20 and 20–30 cm) were analyzed for their chemical properties (soil organic carbon—SOC; soil total nitrogen—STN; inorganic soil carbon—SIC; soil organic matter—SOM; potassium oxide—K₂O; phosphorus pentoxide—P₂O₅; C/N ratio; and pH) and physical properties (volumetric soil water content—VWC; bulk density—BD; and porosity—POR). The results showed that less intensive systems had a higher proportion of legumes, while species diversity, as measured via the Shannon index, was the highest in the Cut4 system. The CG system tended to have the highest SOC and STN at a 0–10 cm depth, with a similar trend observed for SOC_stock at a 0–30 cm depth. The Cut4, HGI and CG systems also had an increased STN_stock. Both grazing systems had the highest P₂O₅ content. A tendency towards a higher BD was observed in the top 10 cm of soil in the more intensive systems. Choosing a management strategy that is tailored to local climate and site conditions is crucial for maintaining grassland stability, enhancing carbon sequestration and promoting long-term sustainability in the context of climate change. Full article

The intestinal microbiota of fish is predominantly composed of prokaryotic microorganisms, with research historically focused on bacteria. In contrast, the role of microeukaryotic organisms in the fish gut remains largely unexplored. This review synthesizes current knowledge on the diversity, ecology, and potential functions of intestinal microeukaryotes, particularly fungi and protozoans, in teleost fish. Fungi, especially Ascomycota and Basidiomycota phyla members, are consistently identified across species and may contribute to digestion, immune modulation, and microbial homeostasis. Protists, though often viewed as pathogens, also exhibit potential commensal or immunoregulatory roles, including the modulation of bacterial communities through grazing. Other eukaryotic taxa, including metazoan parasites, microalgae, and zooplankton, are commonly found as transient or diet-derived members of the gut ecosystem. While many of these organisms remain poorly characterized, emerging evidence suggests they may play essential roles in host physiology and microbial balance. The review highlights the need for improved detection methodologies, functional studies using gnotobiotic and in vitro models, and multi-kingdom approaches to uncover fish gut microeukaryotes’ ecological and biotechnological potential. Full article

Although the benefits of rational grazing by polygastric animals are well known, little is understood about how chicken grazing affects soil biological health and its capacity to store organic matter. This study aimed to assess the impact of long-term free-range chicken grazing in an olive grove on the soil chemical and biochemical properties, including the total organic carbon (TOC), total nitrogen (TN), microbial biomass (Cmic), basal respiration, and microbial community structure, as well as the soil’s capability to stock organic carbon and total nitrogen. A field experiment was conducted in an olive grove grazed by chickens for over 20 years, with the animal load decreasing with distance from the poultry houses. At 20 m, where the chicken density was highest, the soils showed reduced OC and TN contents and a decline in fungal biomass. This was mainly due to the loss of both aboveground vegetation and root biomass from intensive grazing. At 50 m, where grazing pressure was lower, the soil OC, TN, and microbial community size and activity were similar to those in a control, ungrazed area. These findings suggest that high chicken density can negatively affect soil health, while moderate grazing allows for the recovery of vegetation and soil organic matter. Rational management of free-range chicken grazing, particularly through the control of chicken density or managing grazing time and frequency, is therefore recommended to preserve soil functions and fertility. Full article

The Junggar Bactrian camel, a primitive indigenous breed in China, exhibits low reproductive efficiency under natural grazing conditions. This is partly attributed to the development of the epididymis and the quality of semen, both of which directly affect reproductive performance. The epididymis is a key male reproductive organ responsible for sperm storage and transport. However, the gene expression profile of camel epididymal tissue remains poorly understood. In this study, we conducted whole-transcriptome sequencing of epididymal tissues from Junggar Bactrian camels before and after sexual maturity. A total of 683 differentially expressed mRNAs (DEmRNAs) were identified, including TPM2, ITGA5, FASN, and ACP5, of which 415 were upregulated and 268 were downregulated. Additionally, 260 differentially expressed long non-coding RNAs (DELncRNAs), including LOC123611838, LOC105083505, and LOC123614702, were identified, with 113 upregulated and 147 downregulated. An additional 11 differentially expressed microRNAs (DEmiRNAs), including eca-miR-206 and eca-miR-216a, were also detected. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed that key differentially expressed genes (DEGs), including TPM2, ITGA5, DDIT4, FASN, and ACP5, were mainly involved in pathways such as Cell Adhesion Molecules, Phospholipase D signaling, Cytokine–Cytokine Receptor Interaction, and Olfactory Transduction. This study presents a comprehensive whole-transcriptome analysis of the epididymis in Junggar Bactrian camels before and after sexual maturity, identifying key genes and regulatory pathways associated with epididymal development and reproductive function. These findings provide a theoretical foundation and valuable data for future research on reproductive performance and epididymal biology in Bactrian camels. Full article

The Qinghai–Tibet Plateau, a critical ecological barrier and major livestock region, faces deteriorating grasslands and rising forage demand under its harsh alpine climate. Oat (Avena sativa L.), valued for its cold tolerance, rapid biomass accumulation, and ability to thrive in nutrient-poor soils, can expand winter feed reserves and partly alleviate grazing pressure on native rangelands. However, genetic improvement alone has not been sufficient to address the environmental challenges. This issue is particularly severe in the Qaidam Basin, where soil salinization, characterized by high pH, poor soil structure, and low nutrient availability, significantly limits crop performance. Rhizosphere growth-promoting bacteria (PGPR) are environmentally friendly biofertilizers known to enhance crop growth, yield, and soil quality, but their application in the saline soil of the Qaidam Basin remains limited. We evaluated two PGPR application rates (B1 = 75 kg hm⁻² and B2 = 150 kg hm⁻²) on ‘Qingtian No. 1’ oat, assessing plant growth, soil physicochemical properties, and rhizosphere microbial communities. The results indicated that both treatments significantly increased oat productivity, raised the comprehensive growth index, augmented soil organic matter, and lowered soil pH; B1 chiefly enhanced above-ground biomass and fungal community stability, whereas B2 more strongly promoted root development and bacterial community stability. Structural equation modeling showed that PGPR exerted direct effects on the comprehensive growth index and indirect effects through soil and microbial pathways, with soil properties contributing slightly more than microbial factors. Notably, rhizosphere organic matter, fungal β-diversity, and overall microbial community stability emerged as positive key drivers of the comprehensive growth index. These findings provide a theoretical basis for optimizing PGPR dosage in alpine forage systems and support the sustainable deployment of microbial fertilizers under saline soil conditions in the Qaidam Basin. Full article

This study aimed to evaluate the chemical composition of milk from Kazakh mares kept under free-grazing conditions on natural pasture without supplementary feeding. Fifty Kazakh mares were aged 3 to 13 years, kept in herds, and categorized by age, number of foalings, and lactation stage for statistical analysis. Milk samples were collected, chilled, and analyzed on the same day using a Milkoscan FT2. The composition of fat, protein, lactose, total solids, casein, and other components was examined. Statistical analyses, including ANOVA, PCA, CDA, and Pearson correlation analysis, were performed using JMP 17 Pro to assess the effects of lactation stage, mare age, and foaling number and to identify key relationships. The results showed that milk composition was significantly influenced by these factors. Milk from the late-lactation stage contained higher protein, lactose, and total nutrients, while milk from the early-lactation stage had a higher sugar content and acidity. Older mares and those with higher foalings produced milk with higher concentrations of glucose and galactose, while younger mares and those with fewer foalings produced milk with higher fat. A key aspect of this experiment was that all mares grazed exclusively on natural pastures without supplementation, allowing for an objective assessment of their inherent productive potential. These findings deepen the understanding of mare milk composition dynamics in Kazakh breed horses under extensive management, supporting its potential for organic dairy production. Full article

Ecological restoration has increasingly been employed to reverse land degradation and increase carbon (C) sink, especially in ecologically fragile karst areas. Microbial necromass carbon (MNC) constitutes a critical pool within soil organic carbon (SOC), contributing substantially to long-term C sequestration through mineral stabilization. However, its distribution patterns across soil profiles and grassland restoration stages in karst areas remain unclear. To address this knowledge gap, the contents of bacterial necromass C (BNC), fungal necromass C (FNC), and their contributions to SOC were estimated based on glucosamine and muramic acid contents across the soil profile (0–20 cm, 20–40 cm, 40–60 cm, 60–80 cm, and 80–100 cm) for four subalpine restoration stages (grazing enclosure for 5, 11, 17, and 25 years) in the karst region. Our findings demonstrated that both soil depth and grassland restoration stages effectively influenced the BNC and FNC contents. On average, the soil BNC, FNC, and total MNC at the depth of 80–100 cm reduced by 70.50%, 59.70%, and 62.18% compared with in topsoil (0–20 cm), respectively. However, the FNC/BNC ratio gradually increased with the increase in soil depth, which was 43.15% higher at 80–100 cm soil depth than in topsoil, suggesting that the accumulation efficiency of FNC was higher compared to BNC in the deep soil. The BNC, FNC, and MNC were positively correlated with the grassland restoration stage, while FNC/BNC ratio had a negative relationship with the restoration stage (R² = 0.45, p < 0.001). FNC contributed significantly more to SOC (28.6–36.4%) compared to BNC (7.7–9.9%) at all soil depths, indicating that soil fungal necromass has an essential effect on SOC sequestration. The results of the random forest model and distance-based redundancy analysis identified that pH, soil water content, and dissolved organic carbon were the three most essential predictors for the contribution of MNC to SOC. Our study highlights the importance of microbial necromass to SOC accumulation, providing significant scientific implications for the C pool management during the restoration of degraded grasslands in karst regions. Full article

High-latitude regions store substantial amounts of soil organic matter (SOM). Icelandic volcanic soils have exceptional capabilities for SOM accumulation, but recent changes in land use can significantly impact it. Water-extractable organic matter (WEOM) represents a labile SOM pool and serves as a reliable index of SOM dynamics. We assessed the stable carbon (C), stable nitrogen (N), and WEOC (water-extractable organic carbon), as well as WETN (water-extractable total nitrogen), concentrations in soils under different land uses—semi-natural habitats (tundra and wetland) and human-managed areas (intensively and extensively grazed pasturelands and formerly and presently fertilized meadows)—in southeastern Iceland. The results suggest that human-managed sites contain more total C and N but less WEOM per unit of total C or N than semi-natural habitats, except for wetlands. Wetlands exhibited the highest WEOM content. Extensive pasturelands and fertilized meadows are becoming more common in local ecosystems, highlighting the direction of changes in Icelandic grasslands management. Full article

Carbon (C), nitrogen (N), and phosphorus (P) act as pivotal regulators of biogeochemical cycles, steering organic matter decomposition and carbon sequestration in terrestrial ecosystems through the stoichiometric properties of photosynthetic organs. Deciphering their multi-scale spatiotemporal dynamics is central to unraveling plant nutrient strategies and their coupling mechanisms with global element cycling. In the current study, we modeled biogeochemical parameters (C/N/P contents, stoichiometry, and pools) in plant aboveground parts by using the growing mean temperature, total precipitation, total radiation, and maximum normalized difference vegetation index (NDVImax) across nine models (i.e., random forest model, generalized boosting regression model, multiple linear regression model, artificial neural network model, generalized linear regression model, conditional inference tree model, extreme gradient boosting model, support vector machine model, and recursive regression tree) in Xizang grasslands. The results showed that the random forest model had the highest predictive accuracy for nitrogen content, C:P, and N:P ratios under both grazing and fencing conditions (training R² ≥ 0.61, validation R² ≥ 0.95). Additionally, the random forest model had the highest predictive accuracy for C:N ratios under fencing conditions (training R² = 0.84, validation R² = 1.00), as well as for C pool and P content and pool under grazing conditions (training R² ≥ 0.62, validation R² ≥ 0.90). Therefore, the random forest algorithm based on climate data and/or the NDVImax demonstrated superior predictive performance in modeling these biogeochemical parameters. Full article

Grasslands and livestock are essential to support the diversity of soils, plants, and animals. This study analyzes changes that occurred from 2019 to 2022 in two protected pasture areas of the Italian Apennines, designated as UNESCO (area 1) and NATURA2000 (area 2). In each area, three sampling sites were identified and georeferenced, and the soil was studied. Forage quality and productivity were assessed from botanical and chemical perspectives using biomass samples. Adult bovine unit and grazing index were calculated. Soils, classified as Phaeozems in area 1 and Fluvisols in area 2, exhibit a weak structure with an increased risk of compaction and erosion. The height of forage species and vegetal diversity increased during the study, and variations in botanical and chemical composition were observed. Forage productivity averaged 2760 (±1380 SEM) kg DM/ha in area 1 and 3740 (±1160) kg DM/ha in area 2. Animal population declined by 11.4% in area 1 and by 1.14% in area 2, along with a decrease in the number of livestock farms. From a multidisciplinary perspective, improving management would enhance the ecosystem services of pasture areas, including promoting the role of soil as a carbon sink. The results present means of resilience to enhance cultural and naturalistic values of sites in inner Mediterranean ecosystems. Full article

Grasslands, particularly permanent grasslands, provide vital ecosystem services and, therefore, focus a number of management challenges. Grassland management revolves around organizing how livestock graze in both space and time, using various grazing methods. However, international research describes these grazing methods using diverse and sometimes inconsistent terminologies. This lack of standardization may create ambiguity and hinder comparative research on grazing methods. Here, to address this issue, we conducted a literature review aiming to identify common patterns of grazing methods based on shared grazing management criteria. Through multivariate analysis, we analyzed 249 experimental datapoints derived from 102 studies. We ran principal component analysis followed by hierarchical clustering on principal components on seven management criteria. This review identified 4 broad families of grazing methods: continuous grazing, conventional rotational grazing, deferred rotational grazing, adaptative multi-paddock grazing. This work distinguishes rotational from continuous grazing methods, as commonly described in the literature. Furthermore, it identifies adaptative multi-paddock grazing as a distinct and innovative group of rotational grazing. The approach developed here could serve as support to characterize and compare different grazing methods. Full article