Search Results (267)

Grape pomace is an agri-industrial by-product rich in fatty acids with the potential to be used in diets for goats and increase the nutraceutical properties of milk. This study aimed to investigate the effect of incorporating dehydrated grape pomace (DGP) into the diets of Saanen goats on the composition, fatty acid profile, nutritional indices of fatty acids, and antioxidant capacity of their milk. Eight multiparous Saanen goats, averaging approximately four years of age and weighing 41.2 ± 15.7 kg, were used in a double Latin square (4 × 4) design. Diets were formulated with increasing levels of grape pomace (0, 90, 150, and 210 g/kg Dry Matter—DM), replacing cactus. The data underwent analysis of variance using GLM procedure and regression analysis (both linear and quadratic) using REG procedure at a significant level of 5%. The concentration of C18:2 n-6 cis and C18:3 n-3 increased (+20.1 and +15.5%, respectively) with the grape pomace inclusion. There was a reduction in the atherogenic (−24.0%) and thrombogenic (−9.9%) indices of goat milk with the increase in DGP levels. There was a reduction in de novo fatty acids (−10.5%), Δ⁻⁹ desaturase of C14 (−21.8%) and C18 (−9.5%) indexes with the highest level of DGP. There was a quadratic effect for Ferric Reducing Antioxidant Power (FRAP) and a linear effect for phenolic compounds (PC), where the highest values were observed at the estimated levels of 160 g/kg and 210 g/kg DM, respectively. Supplementing dairy goats’ diets with dehydrated grape pomace up to 210 g/kg dry matter enhances the fatty acid profile and nutritional indices of fatty acids of Saanen goat milk without altering its basic composition. As grape production is prevalent in low rainfall regions, pomace may provide an alternative feed in areas with forage production constraints. Additionally, grape residue could establish a link between the wine and dairy sectors for cheese production, expanding markets for farmers. Full article

This study evaluates viticultural parameters of the Golia grape variety in relation to variable climatic conditions over the 2020–2024 period and analyzes their impact on wine quality. The data show significant climatic variability, with warming trends causing earlier flowering and ripening by 11–13 days. Grape production varied depending on climatic conditions, with 2021 and 2024 recording the highest number of shoots per trunk and increased fertility in 2024. Low winter temperature led to reduced bud viability and affected the overall health of the vines and harvest yields. Average annual precipitation, especially from the growing season, significantly influenced actual yield (AY), while higher annual temperatures and sunshine duration (Sun) resulted in lower grape weight. Greater sugar concentrations accumulated in years with higher temperatures, while higher acidity levels registered at lower temperatures. Higher precipitation (Pp) coupled with thermal accumulation promoted higher dry extract and alcoholic strength (AS), significantly enhancing the perception of honey notes (R² > 0.7, p-value < 0.05). Furthermore, higher thermal regimes negatively impacted the expression of delicate aromatic compounds, diminishing specific notes such as rose and exotic fruits. Full article

Interannual variability in climatic conditions represents a major source of uncertainty in cool-climate viticulture, highlighting the need for cultivar-specific assessments of climate–quality relationships. A multi-year on-farm experiment with six monitoring sites has been conducted in vineyards representative of the Tokaj wine region to monitor and assess vintage effect. This study, as the first part of a broader research project evaluating must components, quantifies relationships between climatic indices and key yield- and sugar-related traits (berry weight, total soluble solids, and total dry extract) in Vitis vinifera L. cv. Furmint grown in the Tokaj wine region over three contrasting vintages. Thermal, radiative, and water-availability variables were calculated for discrete phenological phases and statistically analyzed to identify climatic predictors of berry growth and must composition. Berry weight exhibited pronounced vintage sensitivity, showing consistent associations with precipitation-related variables during early developmental stages. In contrast, total soluble solids and total dry extract displayed weaker and less consistent responses to interannual climatic variability. Several widely used heat-accumulation indices showed limited explanatory power, indicating a moderate climatic sensitivity of sugar-related traits in this cultivar. Overall, the results suggest that early-season climatic conditions exert a stronger influence on berry growth than late-season thermal extremes, while compositional parameters related to sugar accumulation remain comparatively stable. These findings highlight the need to incorporate cultivar-specific response functions into statistical models that assess projected climate-change effects on grape quality. Full article

The present research aimed at assessing the effects of the integration of V. vinifera pomace powder, resulting from wine production, into the manufacturing of a sustainable pastry product. Comparisons were made between 9 treatments derived from the factorial combination of 3 tescovine powders (obtained from 3 different manufacturers) added to sponge cakes and 3 concentrations (3%, 5% and 10%), plus a control without tescovine powder addition. Textural, quality and color parameters, as well as bioactive compounds, antioxidant activity and sensory features were determined. The highest porosity and elasto-plastic coefficient were observed for the untreated control, and it was found that the values reduced as grape pomace incorporation increased. The rising application of tescovine powder enhanced the textural parameters of sponge cakes compared to the untreated control, with the highest average level observed with Natur Tanya integration. Resilience, cohesiveness and elasticity decreased as the concentration of grape pomace rose to 10%. Gummosity and chewability displayed increasing trends from the untreated control to the 10% tescovine powder addition. Dry matter content, acidity, sugars, mineral substances and ash increased from the control to the highest grape pomace integration, contrary to the pH trend. With rising tescovine powder incorporation, the sponge cake color components L* and b* decreased compared to the untreated control, whereas the component a* showed increasing values in the cake crumb but decreasing values in the crust. The highest antioxidant activity and compound levels were recorded with the 10% Fiber Foods grape by-product addition. Most sensory characteristics showed a decreasing trend with increasing tescovine powder integration from 3% to 10% in the pastries analyzed. This study demonstrated the benefits of adding tescovine by-product to sponge cakes to manufacture innovative foods with high nutritional value. Full article

Grapevine leafroll-associated virus (GLRaV) is a globally widespread disease that causes substantial economic losses in the wine industry. In severely affected vineyards, GLRaV can reduce grape yield by 20–40%. This study aimed to evaluate the effects of GLRaV infection on polyphenolic and volatile organic compound contents, as well as on the sensory profiles of the resulting wines. A comparative analysis was conducted between GLRaV-3-infected and healthy Cabernet Sauvignon grapes and their corresponding wines. Results show GLRaV-3 significantly alters grape and wine quality. In infected grapes, sugar content decreased while titratable acidity increased. Polyphenol composition was notably altered: phenolic acids and flavonols increased by 22.46% and 15.27%, respectively, whereas flavanols decreased by 17.86%. The levels of aldehydes and C₆ compounds also rose significantly in the berries. Wines produced from infected grapes showed lower alcohol content and reduced dry extract. Phenolic acids and flavanols were decreased, but total flavonols increased by 12.54%. Among volatile compounds, alcohols, phenols, and fatty acids were elevated, while esters declined by 13.36%. These chemical changes directly influenced sensory attributes. Compared with wines from healthy grapes, those from infected grapes exhibited improved tannin texture and longer aftertaste. However, they were inferior in color intensity, aroma intensity, body fullness, and varietal typicity. Full article

The growing interest in precision agriculture has led, in recent years, to an increase in the adoption of Internet of Things (IoT) technologies in the service of smart agriculture to optimize agricultural production processes through the monitoring of environmental conditions and prevent food loss. This work stems from research conducted as part of the Tech4You project, where the enabling digital technologies developed in Spoke 6 contribute to the advanced solutions envisaged by Spoke 3 to facilitate the transition to a sustainable agrifood system. In particular, we present the design and evaluation of a multi-hop Device-to-Device (D2D) communication architecture that leverages Long Range (LoRa) technology, specifically designed for monitoring vineyards in the context of passito wine production. The proposed framework addresses the challenge of monitoring mobile containers for grapes during the drying phase, a critical stage in which inadequate temperatures and humidity can promote the growth of fungi and the formation of mycotoxins. The integration of simulation-based performance evaluation with a multi-layer system architecture is presented in this work. The objective is to compare the performance of different routing strategies in choosing data forwarding paths to the gateway. The simulation results show that the proposed routing strategy, which is based on learning but also focuses on energy consumption, offers good performance. In particular, it achieves packet delivery rates of over $92\%$ and preserves over $95\%$ of active nodes after 2 h of operation. Energy-aware routing strategies also perform well compared to those that only consider the distance from the destination, but overall, the proposed strategy achieves a better trade-off on the metrics analyzed. Full article

Climate change is modifying ecoclimatic conditions, including temperature, solar radiation, and water availability, with significant impacts on grapevine phenology, berry ripening, and the polyphenolic composition of grapes cultivated in temperate regions. The influence of different meteorological conditions during ripening on the polyphenolic composition of Grignolino grapes grown in a hilly environment was investigated. Grapes were collected, over three vintages, from three vineyards differing in their vine age and bunch microclimate due to having different vineyard aspects. We considered a comparable berry weight, moderate rainfall and cool conditions before veraison, followed by a warm and dry pre-harvest stage that enhanced the phenolic and especially the anthocyanin index in the grapes (e.g., 360 mg kg⁻¹ in 2021 versus 260 mg kg⁻¹ in 2020). Intense heat and dry conditions reduced the berry weight, leading to an increase in both flavonols and hydroxycinnamoyl tartaric acids, particularly in the younger, southwest-exposed vineyard. Older vines with a cooler aspect were the most resilient to different meteorological conditions, while young vines showed greater variability over the years. The phenolic composition was strongly influenced by the intensity and the timing of thermal stress, and eventually on limited water availability during ripening; it also depended on the vine age and the vineyard microclimate determined by the hillside aspect. This knowledge may support adaptive strategies to preserve grape quality under climate change. Full article

Winemakers have to deal with enzymatic browning caused by a grape polyphenol oxidase called tyrosinase. Due to the problems related to sulphur dioxide and its use in winemaking, oenologists need alternative and effective treatments for inhibiting enzymatic browning. This research studies ascorbic acid, glutathione (pure and in the form of a specific inactivated dry yeast rich in glutathione), and bioprotection by a selected strain of Metschnikowia pulcherrima as alternatives to SO₂ for preventing enzymatic browning, following a methodology developed in previous works. All the studied treatments resulted in a significant reduction in enzymatic browning. More specifically, all treatments significantly reduced the Vmax of the apparent tyrosinase activity, indicating their potential to protect against enzymatic browning and thus allowing for a reduction in the doses of SO₂ added to the wine. Full article

Large amounts of agro-industrial residues—such as grape pomace, olive mill wastewater, wheat straw, and cheese whey—pose disposal challenges and generate substantial environmental and economic burdens. Incorporating these by-products into ruminant diets may reduce feeding costs while supporting sustainability within a circular bioeconomy. Two experiments were conducted to evaluate: (i) the characteristics of a mixed raw agro-industrial by-product silage (BPS) in cylindrical bale silos (50 kg) and (ii) its effects as dietary supplementation on metabolic profile, oxidative status, milk yield, and milk quality in lactating goats. The BPS was formulated from raw wheat straw, grape pomace, olive mill wastewater, and cheese whey and tested at a 30% inclusion level (dry-matter basis). The combined by-products produced in 50 kg cylindrical bale silos were positively characterized and exhibited a nutritionally suitable silage with relevant antioxidant potential. Dietary BPS improved oxidative status, as evidenced by increased TAS and vitamin E and reduced ROMs, and positively influenced lipid and immunological blood markers of goats. Milk yield was higher in goats receiving the BPS. Moreover, milk quality was improved through a more favorable fatty acid profile, higher vitamin E content, reduced cholesterol levels, and enhanced oxidative stability, as evidenced by lower TBARS values. Overall, BPS supplementation enhanced animal health indicators and milk nutritional properties, demonstrating that this silage can contribute to more sustainable dairy goat production while offering potential benefits for human nutrition. Full article

In order to reduce fungal contamination in grapes and increase the dehydration rate for producing raisins, the development of alternative technologies that do not compromise product safety and quality is required. This study examined the impact of innovative pre-drying methods using aqueous ozone (10 min-4.1 mg O₃ L⁻¹) and UV-C light (30.3 kJ m⁻² UV-C) on the incidence of Aspergillus carbonarius, as well as on air-drying kinetics and ultrastructure of epicuticular waxes in Sultanina grapes, when applied either individually or sequentially. The effect of the pre-treatments on the colour of the dehydrated grapes was also assessed. Grapes pre-treated with 30.3 kJ m⁻² UV-C and 10 min-4.1 mg O₃ L⁻¹ + 30.3 kJ m⁻² UV-C showed a lower incidence of A. carbonarius in storage at 20 ± 1 °C than those exposed to aqueous ozone (30 and 8% lower infection compared to the non-pretreated fruit at 15-day storage, respectively). Although the combined pre-treatment did not significantly improve the fungus inhibition with respect to the individual UV-C application, it allowed a higher dehydration rate during the drying process at 60 ± 1 °C. The drying time was reduced by ~31% compared to non-pretreated fruit, a result slightly lower than that achieved with the traditional chemical pre-treatment of ethyl oleate-K₂CO₃ (~39%). This enhancement in drying rate was partly attributed to marked alterations in the grape’s epicuticular wax layer. UV-C and the combined pre-treatment helped in reducing the browning of raisins. Therefore, the combined application of ozone and UV-C light could be an environmentally friendly alternative for both improving the microbiological quality of grapes and accelerating the drying process. Full article

The rapid development of viticulture in subtropical regions represents a significant achievement in China’s table grape industry over the last two decades. However, insufficient winter chilling in these areas often leads to inadequate dormancy, which compromises nutrient translocation and storage in grapevines. Insufficient chilling accumulation results in asynchronous budbreak and reduced cane quality. In this study, ‘Shine Muscat’ grapevines were used to systematically evaluate how different defoliant agents affect budbreak characteristics from the perspective of nutrient translocation and storage. The results indicated that applications of ethephon or urea alone, as well as their combinations with boric acid, yielded unstable effects, often causing primary bud necrosis, decreased flower formation rates, and phytotoxicity. In contrast, the combination of lime sulfur and boric acid exhibited a remarkable synergistic effect, significantly promoting dry matter and starch accumulation in canes while enhancing the budbreak speed, uniformity, and flower cluster formation rate. Further experiments with varying concentrations of lime sulfur combined with 0.2% boric acid revealed that utilizing 2% lime sulfur in this combination produced the most pronounced effects, achieving the highest dormancy-breaking efficacy under conventional cultivation conditions. This treatment was used for the first time to produce a second crop during off-season cultivation. The dual effects of dormancy release and bud promotion achieved via this approach represent a reliable solution in high-quality and efficient grape production in subtropical regions. Full article

This study evaluated the effects of replacing soybean hulls with dried grape pomace (DGP) on feed intake, milk production and composition, and rumen N degradability in dairy cows. A 3 × 3 Latin square design was used with three rumen-fistulated Holstein cows, three corn silage-based diets containing 0%, 3%, or 6% of DGP, and three 23-day periods. Measurements were taken from days 15 to 21, with in situ incubations of two protein sources during the last 2 days. At trial end, cows continued on experimental diets to determine the degradability of soybean hulls and DGP. Compared to soybean hulls, DGP presented higher fiber content and lower in situ rumen dry matter and N degradability. Condensed tannin disappearance from DGP increased over time, reaching almost 50% after 48 h. Diet did not affect feed intake, milk production, and composition, except for milk urea N and ruminal total short-chain fatty acids concentration, which showed a quadratic response, suggesting both imbalances between fermentable energy and rumen degradable protein and potential effects of DGP bioactive compounds on rumen function. The N degradation rate of rapeseed meal was unaffected, but soybean meal degradability tended to increase with higher DGP inclusion. This was also detected when combining protein sources across diets. Although DGP influenced rumen function, further research integrating omics and detailed microbiota profiling is needed. Overall, despite its low energy content and rumen degradability, DGP comprises a sustainable feed resource for high-producing animals, supporting circular economy approaches and mitigating the environmental impacts of grape pomace disposal. Full article

Grapevines face the dual challenge of sustaining yield and fruit quality under arid and increasingly variable environmental conditions. This study characterized the phenotypic variability and multi-year stability of 49 grapevine (Vitis spp.) accessions conserved in the Chincha germplasm bank over three consecutive growing seasons, with the aim of identifying promising material for table grape, pisco (a traditional grape-based distilled spirit from Peru), and wine production. Morphological traits (cluster weight, berry weight and dimensions), colorimetric parameters (CIELAB), and physicochemical attributes (moisture, dry matter, soluble solids, pH, titratable acidity, maturity index, and reducing sugars) were evaluated. Multivariate analyses (PCA, hierarchical clustering), genotype × environment interaction models (AMMI and GGE), stability indices (ASV and WAASBY), and assessments of interannual stability were applied, together with a multi-criteria selection index tailored to the intended end use. The results revealed two contrasting phenotypic profiles: one characterized by high berry volume/weight and elevated water content and another with smaller berries but higher dry matter, sugars, balanced acidity, and superior maturity indices. Genotypic effects were predominant for size-related traits such as berry weight, whereas titratable acidity and reducing sugars exhibited a more pronounced genotype × year interaction, supporting the use of AMMI models and the WAASBY index to select genotypes that are both productive and stable. The ranking identified accessions PER1002061, PER1002062, and PER1002168 as outstanding candidates for table grape production; PER1002076, PER1002097, and PER1002156 for pisco; and PER1002122, PER1002131, PER1002135, and PER1002098 as accessions with high oenological potential. Overall, these findings highlight the value and diversity of Peruvian grapevine germplasm and provide a foundation for breeding programs targeting varieties adapted to specific market niches, including table grape, wine, and pisco. Full article

The efficient detection of grape leaf nutrient parameters, including chlorophyll content, represented by soil and plant analysis development (SPAD), leaf nitrogen content (LNC), leaf potassium content (LKC), fresh weight water content (FWC), and dry weight water content (DWC), is crucial in precision agriculture. This study introduces a modeling framework that integrates hyperspectral preprocessing, feature selection, and multimodal data fusion. This framework enhances feature representation and model robustness by fusing spectral features (Ref), vegetation indices (VIS), and color and texture features from hyperspectral and red, green, and blue (RGB) images. Comparative experiments based on partial least squares regression (PLSR), Gaussian Process regression (GPR), and Bayesian Ridge regression (BRR) demonstrate that with a limited sample size, the PLSR and BRR models exhibit superior predictive performance and stability. However, during the optimization process, the performance improvement of the GPR model was the greatest (with R² increasing by up to 31.9%). Among the features, vegetation indices showed relatively high correlations with various traits. For image features, hyperspectral texture characteristics performed best, while color features from RGB images contributed significantly. Following preprocessing, feature selection, and feature combination, the performance of all models, except for DWC, improved progressively. Notably, feature selection significantly increased model accuracy. These findings indicate that multi-stage collaborative optimization strategies can be employed for the precise prediction of grape leaf functional traits. Full article

To tackle grape branch and leaf waste and alleviate global feed shortages, this study tested silage made from Xinjiang ‘Seedless White’ grape foliage. Three treatments were established: CK (control, only grape branches and leaves), PL (inoculated with 5 × 10⁶ CFU·g⁻¹ fresh weight Lactiplantibacillus plantarum), and PLC (inoculated with 5 × 10⁶ CFU·g⁻¹ L. plantarum and 0.3% cellulase). Silages were fermented at 18–23 °C and analyzed on days 7, 15, 30, and 60. PLC reduced dry matter loss in the late fermentation stage, while lowering Neutral detergent fiber (NDF) and Acid detergent fiber (ADF) contents to solve the high-fiber issue of grape foliage silage. It also maintained a lower pH in the mid-to-late stage and higher Lactic acid (LA) content to ensure anti-spoilage. Microbiologically, PLC had the highest Lactiplantibacillus abundance on day 7; on day 60, its Simpson index was higher, meaning stronger microbial community stability. Firmicutes replaced Cyanobacteria as the new dominant phylum, with Lactiplantibacillus remaining the absolute dominant genus, and the growth of molds and yeasts was effectively inhibited. In conclusion, the combined application of L. plantarum and cellulase enhances the quality of grape branch and leaf silage. This study turns low-value grape branches and leaves into high-quality feed, providing support for grape branch and leaf resource utilization and helping alleviate global feed shortages. Full article