Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Article Types

Countries / Regions

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Search Results (270)

Search Parameters:
Keywords = flavoring agents

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
15 pages, 3222 KiB  
Article
Process Optimization of Thawed Cloudy Huyou Juice Clarification Using a Composite of Carboxymethyl Chitosan and Sodium Alginate
by Peichao Zhang, Liang Zhang, Xiayu Liu, Yuxi Wang, Jiatong Xu, Pengfei Liu and Boyuan Guan
Foods 2025, 14(15), 2658; https://doi.org/10.3390/foods14152658 - 29 Jul 2025
Viewed by 143
Abstract
Cloudy huyou juice is increasingly popular for its unique flavor, but flocculent precipitation after cold storage and thawing affects its sensory quality and increases production costs. This study optimized the clarification of thawed cloudy huyou juice using a composite of carboxymethyl chitosan (CC) [...] Read more.
Cloudy huyou juice is increasingly popular for its unique flavor, but flocculent precipitation after cold storage and thawing affects its sensory quality and increases production costs. This study optimized the clarification of thawed cloudy huyou juice using a composite of carboxymethyl chitosan (CC) and sodium alginate (SA), prepared via ionic and covalent crosslinking. The composite was characterized by SEM, FTIR, and thermal analysis. Transmittance was used to evaluate clarification performance. The effects of dosage, adsorption time, and temperature were first assessed through single-factor experiments, followed by optimization using a Box–Behnken response surface methodology. The composite significantly improved clarity (p < 0.05), reaching 85.38% transmittance under optimal conditions: 22 mg dosage, 80 min time, and 38 °C. The composite dosage and temperature were the most influential factors. Reusability tests showed declining performance, with the transmittance dropping to 57.13% after five cycles, likely due to incomplete desorption of adsorbed compounds. These results suggest that the CC-SA composite is an effective and reusable clarifying agent with potential for industrial applications in turbid fruit juice processing. Full article
(This article belongs to the Section Food Biotechnology)
Show Figures

Figure 1

23 pages, 3376 KiB  
Article
Physicochemical and Instrumental Flavor Analysis of Plant-Based Drinks with Plant Powder Additions
by Joanna Kolniak-Ostek, Agnieszka Kita, Davide Giacalone, Laura Vázquez-Araújo, Luis Noguera-Artiaga, Jessica Brzezowska and Anna Michalska-Ciechanowska
Foods 2025, 14(15), 2593; https://doi.org/10.3390/foods14152593 - 24 Jul 2025
Viewed by 315
Abstract
This study explored the use of fruit- and herb-based powders as fortifying agents in soy- and oat-based beverages. Developed using a New Product Development approach, the powders were derived from underutilized plants rich in bioactives but with limited sensory appeal. Formulations included powders [...] Read more.
This study explored the use of fruit- and herb-based powders as fortifying agents in soy- and oat-based beverages. Developed using a New Product Development approach, the powders were derived from underutilized plants rich in bioactives but with limited sensory appeal. Formulations included powders from both widely available fruits, such as apple and pear, chosen for their accessibility and economic relevance, and less commonly consumed fruits, such as Japanese quince, rosehip, and rhubarb, which are often discarded due to sour or astringent flavors. Processing these into powders helped mask undesirable sensory traits and enabled incorporation into beverage matrices. Physicochemical analyses confirmed their technological suitability, while high polyphenol content indicated potential health benefits. Importantly, no process contaminants (furfural, 5-hydroxymethyl-L-furfural, and acrylamide) were detected, supporting the powders’ safety for food use. The integrated application of an electronic tongue and nose enabled objective profiling of taste and aroma. The electronic tongue distinguished taste profiles across formulations, revealing matrix-dependent effects and interactions, particularly with trehalose, that influenced sweetness and bitterness. The electronic nose provided consistent aroma differentiation. Overall, the results highlight the potential of these underutilized plant powders as multifunctional ingredients in plant-based beverage development. They support product innovation aligned with consumer expectations for natural, health-promoting foods. Future work will include sensory validation with consumer panels. Full article
(This article belongs to the Section Nutraceuticals, Functional Foods, and Novel Foods)
Show Figures

Figure 1

12 pages, 2783 KiB  
Article
Physicochemical, Nutritional, and Structural Characterization of a Novel Meat-Based Hummus
by Meena Goswami, Rishav Kumar, Xin M. Teng, Ravi Jadeja, Darren Scott, Morgan Pfeiffer, Gretchen G. Mafi, Vikas Pathak and Ranjith Ramanathan
Foods 2025, 14(14), 2507; https://doi.org/10.3390/foods14142507 - 17 Jul 2025
Viewed by 391
Abstract
The objective was to characterize physicochemical, nutritional, and structural properties of a novel meat-based hummus. This product was created by substituting 50% of chickpea paste with mutton. The meat-based hummus contained 0.4% sodium acid sulfate as an antimicrobial agent. The pH values of [...] Read more.
The objective was to characterize physicochemical, nutritional, and structural properties of a novel meat-based hummus. This product was created by substituting 50% of chickpea paste with mutton. The meat-based hummus contained 0.4% sodium acid sulfate as an antimicrobial agent. The pH values of traditional hummus were greater than those of the meat-based hummus. There was no significant difference in day 0 total plate count between plant- and meat-based hummus; however, the total plate count on day 7 was significantly (p < 0.05) lower in the meat-based hummus than plant-based hummus due to antimicrobial addition. Instrumental color analysis showed greater lightness (L* values) and yellowness values for traditional hummus compared to the meat-based hummus. The meat-based hummus had 66% greater protein than traditional hummus. Scanning electron microscopy revealed a porous, gel-like structure in plant-based hummus, while meat-based hummus showed a dense, fibrous network. The flavor, creaminess, grain properties, and mouth coating scores of meat-based hummus were greater than those of traditional chickpea hummus. The study indicated that meat-based hummus can be developed by incorporating 50% cooked minced mutton. Creating innovative meat-based products like meat hummus offers the benefits of both plant-based and animal-based diets, making it a good option for flexitarians. Full article
Show Figures

Figure 1

14 pages, 4310 KiB  
Article
Effect of Different Gelling Agents on the Properties of Wine Jellies Prepared from Aromatic Grape Varieties
by Radek Sotolář, Petr Bača, Vladimír Mašán, Petr Vanýsek, Patrik Burg, Tomáš Binar and Oldřiška Sotolářová
Processes 2025, 13(6), 1893; https://doi.org/10.3390/pr13061893 - 15 Jun 2025
Viewed by 443
Abstract
Wine jelly is regarded as a delicacy in many countries and is commonly utilized in grande cuisine. Recently, its popularity has increased among consumers due to its dietary properties and the presence of health-promoting compounds such as antioxidants. Its natural origin and the [...] Read more.
Wine jelly is regarded as a delicacy in many countries and is commonly utilized in grande cuisine. Recently, its popularity has increased among consumers due to its dietary properties and the presence of health-promoting compounds such as antioxidants. Its natural origin and the ability to reflect local traditions and consumer preferences further enhance its appeal. This study aimed to compare the compositional and sensory characteristics of wine jellies prepared using three different gelling agents and four aromatic grape varieties, with the goal of preserving varietal aroma in the final products. White wines from Pálava and Moravian Muscat and red wines from Agni and Rosa were used. The selected gelling agents were agar, vegan gelatin, and traditional gelatin. Basic analytical parameters were assessed in both the wines and the resulting jellies. Sensory evaluation was conducted by trained panelists, assessing consistency, appearance (clarity), taste, and bouquet. Confectionery-grade jelly from red wines demonstrated the best consistency, while gelatin jellies from white wines showed superior clarity. Due to a preference for sweeter flavors, jellies from red wines were favored across all variants. The strongest varietal bouquet was observed in Moravian Muscat samples, irrespective of the gelling agent used. The optimal choice of gelling agent depends on the target quality attributes. Gelatin is preferred for firmness and clarity, while vegan gelatin is ideal for preserving aroma and achieving a balanced sensory profile. Full article
Show Figures

Figure 1

19 pages, 1276 KiB  
Article
Design and Production of an Instant Coffee Product Based on Greek Coffee Oil: Study of the Effect of Storage Conditions on Product Aroma and Quality
by Efimia Dermesonlouoglou, Vassiliki Palaioxari-Kampisiouli, Dimitrios Tsimogiannis and Petros Taoukis
Beverages 2025, 11(3), 88; https://doi.org/10.3390/beverages11030088 - 9 Jun 2025
Viewed by 897
Abstract
The objective of this study was to obtain and evaluate a coffee aroma extract/oil with sensorial attributes close to the original brew of Greek coffee for use in an instant Greek coffee powder. The oil was obtained directly from commercial Greek coffee by [...] Read more.
The objective of this study was to obtain and evaluate a coffee aroma extract/oil with sensorial attributes close to the original brew of Greek coffee for use in an instant Greek coffee powder. The oil was obtained directly from commercial Greek coffee by solid-liquid extraction using hexane as a solvent and treated with a series of hexane-ethanol mixtures (0:10, 1:4, 1:9) to remove the intense roasted flavor of the crude coffee oil obtained by hexane; the de-oiled coffee was used for the recovery of water-soluble compounds, and the produced water extract was freeze-dried. The aromatic volatiles of the coffee oil samples were analyzed by using a purge-and-trap device coupled to GC-MS, as well as sensory analysis. The instant Greek coffee powder was produced by mixing the freeze-dried base (74.4%) with the extract derived after treatment of the crude oil with hexane-ethanol mixture 1:4 (18.2%) and foaming agent (7.4%). Two different materials were studied as bases: instant coffee (F3Gr-D) and ground Greek coffee (reference sample, CGr). The shelf-life stability of the produced powders was examined at three storage temperatures (25, 45, 60 °C). Instrumental analysis (purge-and-trap GC-MS) of aroma and sensory analysis (aroma, taste, staling, total sensory quality on a 1–9 hedonic scale) was conducted. Aroma loss (furfuryl alcohol, furfural, dimethyl pyrazines, ethyl methyl pyrazines) and scores for sensory attributes during storage were modeled using 1st and 0-order reaction kinetics, respectively. The storage temperature effect was expressed by the Arrhenius model (activation energy Ea). According to the results, the developed instant coffee powder presented satisfactorily the aroma characteristics of regular Greek coffee. The shelf life for the instant Greek coffee powder was estimated as 80 days (air packed) (based on 20% retention of furfuryl alcohol that was the most abundant aromatic volatile of Greek coffee aroma, ground as well as extract oil). Full article
(This article belongs to the Special Issue New Insights into Artisanal and Traditional Beverages)
Show Figures

Figure 1

16 pages, 4519 KiB  
Article
Effects of Low Green Light Combined with Different Red and Far-Red Light Ratios on the Growth and Secondary Metabolites of Cilantro (Coriandrum sativum L.)
by Manuel Mayam Miranda Sotelo, Yuan-Kai Tu, Pearl Pei-Chun Chang, Wei Fang and Hsing-Ying Chung
Agronomy 2025, 15(6), 1363; https://doi.org/10.3390/agronomy15061363 - 31 May 2025
Cited by 1 | Viewed by 663
Abstract
Plant factories offer a promising opportunity for fresh food production due to their minimal land requirements. Among the adjustable factors in the production system of plant factories, light serves as a critical element, significantly influencing both crop yield and quality. Cilantro, a prevalent [...] Read more.
Plant factories offer a promising opportunity for fresh food production due to their minimal land requirements. Among the adjustable factors in the production system of plant factories, light serves as a critical element, significantly influencing both crop yield and quality. Cilantro, a prevalent culinary herb and a traditional flavoring agent, plays a crucial role in Taiwanese gastronomy. This research investigated cilantro plants grown under nine different light treatments with varying red to far-red ratios and green light percentages over a 49-day period. Results demonstrate that maximum fresh and dry biomass accumulation in both shoot and root tissues occurred under treatments with red to far-red ratios of approximately of 1.8 combined with medium green light intensity. Conversely, medium far-red ratios negatively affected lutein and carotenoid concentrations in foliar tissues. Carotenoid biosynthesis exhibited an inverse relationship with green light intensity, with lower green light percentages corresponding to significantly higher carotenoid concentrations. In terms of energy efficiency, a red to far-red ratio of approximately 1.8 yielded the highest energy yield (g kWh−1) and photon yield (g mol−1), indicating optimal energy conversion efficiency under this spectral composition. In conclusion, this study demonstrates that cilantro cultivation under R53G05B13FR29 spectral composition (53% red, 5% green, 13% blue, 29% far-red) with a 49-day production cycle maximizes biomass while optimizing energy utilization efficiency. Full article
Show Figures

Figure 1

14 pages, 3580 KiB  
Article
Cinnamomum cassia Alleviates Neuropsychiatric Lupus in a Murine Experimental Model
by Georges Maalouly, Youakim Saliba, Joelle Hajal, Anna Zein-El-Din, Luana Fakhoury, Rouaa Najem, Viviane Smayra, Hussein Nassereddine and Nassim Fares
Nutrients 2025, 17(11), 1820; https://doi.org/10.3390/nu17111820 - 27 May 2025
Viewed by 539
Abstract
Background: The pathogenesis of neuropsychiatric lupus erythematosus (NPSLE) is very complex and is associated with neuroinflammation and blood–brain barrier compromise. Experimental investigations of NPSLE have classically relied on spontaneous models. Recently, TLR7 agonist-induced lupus has been shown to exhibit similar neuropsychiatric manifestations to [...] Read more.
Background: The pathogenesis of neuropsychiatric lupus erythematosus (NPSLE) is very complex and is associated with neuroinflammation and blood–brain barrier compromise. Experimental investigations of NPSLE have classically relied on spontaneous models. Recently, TLR7 agonist-induced lupus has been shown to exhibit similar neuropsychiatric manifestations to spontaneous ones. Cinnamon is a widespread spice and natural flavoring agent. It has been proven to modulate vascular endothelial tight junctions, neuroinflammation, and autoimmunity pathways, but it has never been tested in relation to lupus. Hypothesis/Purpose: In this pilot study, we aimed to explore the disease-modifying effect of Cinnamomum cassia on NPSLE in a TLR7 agonist-induced model. Study Design: An experimental design was followed in this study. Methods: Lupus was induced in C57BL/6J female mice via the direct application of imiquimod, a TLR7 agonist (5% imiquimod cream, 1.25 mg three times weekly), to the skin. Mice were divided into five groups (n = 8 per group): a sham group (S), a sham group supplemented with cinnamon (SC), an imiquimod-treated group (L), an imiquimod-treated group supplemented with cinnamon starting from induction (LC), and an imiquimod-treated group supplemented with cinnamon beginning two weeks prior to induction (CLC). This protocol was followed for six consecutive weeks. Cinnamomum cassia powder was administered orally at 200 mg/kg, 5 days per week. Results: Behavioral alterations were significantly ameliorated in the CLC group compared to lupus mice. Neuronal shrinkage and nuclear chromatin condensation were visible in the hippocampal cornu ammonis and dentate gyrus zones of lupus mice, with an increased expression of TLR7 and NLRP3, versus significantly less neurodegeneration and TLR7 and NLRP3 expression in the CLC group. In addition, the expression of the blood–brain barrier endothelial cell tight junction proteins claudin-1, occludin, and ZO-1 was abnormally modified in lupus mice and was restored in the CLC group. Moreover, while the cell–cell border delocalization of claudin-1 was documented in cultured blood–brain barrier endothelial cells treated with the plasma of lupus mice to a punctate intracytoplasmic fluorescence pattern, only cells treated with the plasma of the CLC group exhibited a complete reversal of this redistribution of claudin-1. Finally, cinnamaldehyde seemed to interact with TLR7 at multiple sites. Conclusions:Cinnamomum cassia seems to alleviate the pathogenesis of NPSLE. Supplementation with Cinnamomum cassia could be of great interest to modulate the activity and severity of the disease. Full article
(This article belongs to the Section Nutritional Immunology)
Show Figures

Graphical abstract

34 pages, 1390 KiB  
Review
A Comprehensive Review of Edible Flowers with a Focus on Microbiological, Nutritional, and Potential Health Aspects
by Angela Daniela Carboni, Tiziana Di Renzo, Stefania Nazzaro, Pasquale Marena, Maria Cecilia Puppo and Anna Reale
Foods 2025, 14(10), 1719; https://doi.org/10.3390/foods14101719 - 12 May 2025
Viewed by 1969
Abstract
Edible flowers have been used since ancient times directly as food, flavoring agents, and garnish in food products, and are now reappearing in modern cuisine. Edible flowers have gained popularity due to changing consumer habits focused on healthier food options. In addition to [...] Read more.
Edible flowers have been used since ancient times directly as food, flavoring agents, and garnish in food products, and are now reappearing in modern cuisine. Edible flowers have gained popularity due to changing consumer habits focused on healthier food options. In addition to contributing to the esthetics and flavor of various dishes, edible flowers are now recognized for their nutritional value, as they contain bioactive components with different health benefits. However, a significant concern regarding edible flowers is the potential contamination by undesirable microorganisms. Since edible flowers are often consumed fresh or minimally processed, they can pose a microbiological risk. Edible flowers may be susceptible to contamination by various pathogenic microorganisms, particularly Bacillus spp., Enterobacter spp., Salmonella spp., and Staphylococcus aureus. In addition, mycotoxin-producing fungi, such as Aspergillus, Penicillium, Alternaria, or Fusarium, can be found in various flowers. Good agricultural practices, hygienic handling, and appropriate storage are essential to reduce contamination and guarantee the safe consumption of edible flowers. Since current investigations on the microbiological safety aspects of edible flowers are scarce, this review aims to provide an overview of the consumption of edible flowers and a discussion of their uses, health benefits, and risks, focusing on microbiological aspects. Full article
(This article belongs to the Special Issue Feature Reviews on Food Microbiology)
Show Figures

Graphical abstract

23 pages, 4959 KiB  
Article
Characterization of Key Metabolic Markers in Hongqujiu Across Different Aging Years Using Metabolomics
by Yiyang Cai, Sunan Yan, Simei Huang, Bin Yang, Wenlan Mo, Lishi Xiao, Xiangyou Li and Zhiwei Huang
J. Fungi 2025, 11(5), 353; https://doi.org/10.3390/jof11050353 - 2 May 2025
Viewed by 547
Abstract
Hongqujiu, one of the three principal varieties of yellow wine, is a traditional fermented beverage originating from China that employs Hongqu as the fermentation agent. In this study, an untargeted metabolomics approach based on gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (LC-MS) [...] Read more.
Hongqujiu, one of the three principal varieties of yellow wine, is a traditional fermented beverage originating from China that employs Hongqu as the fermentation agent. In this study, an untargeted metabolomics approach based on gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (LC-MS) was applied to systematically analyze the volatile compounds (VOCs) and non-volatile compounds (NVCs) in Hongqujiu across different aging years for the first time. The analysis identified a total of 262 VOCs and 2564 NVCs in samples of Hongqujiu aged for six distinct years. Based on metabolic differences, the samples were categorized into two groups: the low-year group (5-year, 6-year) and the high-year group (8-year, 10-year, 15-year, 20-year). Nineteen VOCs (e.g., 4-amino-butyric acid and diethanolamine) and thirty NVCs (e.g., palmitoylethanolamide and erinacine D) were identified as key differential metabolites distinguishing the low-year group from the high-year group. The higher-year group is enriched with a variety of substances with different flavors or biological activities, such as sugar derivatives, amino acids and their complexes, organic acids and their intermediate metabolites, steroids and terpenoid compounds, lipids and their derivatives, nitrogen-containing heterocycles, and aromatic compounds. The accumulation of these substances not only shapes the unique and rich flavor characteristics of aged red rice wine (such as the caramel aroma and umami peptide flavor), but also endows red rice wine with potential health benefits due to the physiological regulatory functions of some active ingredients. This study contributes to a deeper understanding of the composition and dynamic variations in metabolites in Hongqujiu, offering a scientific foundation for identifying aged Hongqujiu and conducting further research to enhance its quality. Full article
(This article belongs to the Special Issue Monascus spp. and Their Relative Products)
Show Figures

Figure 1

19 pages, 4455 KiB  
Article
Chemical Composition, Chemometric Analysis, and Sensory Profile of Santolina chamaecyparissus L. (Asteraceae) Essential Oil: Insights from a Case Study in Serbia and Literature-Based Review
by Biljana Lončar, Mirjana Cvetković, Milica Rat, Jovana Stanković Jeremić, Jelena Filipović, Lato Pezo and Milica Aćimović
Separations 2025, 12(5), 115; https://doi.org/10.3390/separations12050115 - 2 May 2025
Cited by 1 | Viewed by 700
Abstract
The flowers of Santolina chamaecyparissus have a distinct aroma and taste, with a wide range of applications in medicine, food, and packaging. Its essential oil offers numerous health benefits, including antioxidant, hepatoprotective, anticancer, antidiabetic, spasmolytic, anti-inflammatory, immunomodulatory, antimicrobial, and antiparasitic properties. Additionally, it [...] Read more.
The flowers of Santolina chamaecyparissus have a distinct aroma and taste, with a wide range of applications in medicine, food, and packaging. Its essential oil offers numerous health benefits, including antioxidant, hepatoprotective, anticancer, antidiabetic, spasmolytic, anti-inflammatory, immunomodulatory, antimicrobial, and antiparasitic properties. Additionally, it is used as a flavoring agent in food and beverages and as a natural preservative in edible coatings for food packaging. This study investigates the chemical composition and sensory properties of the S. chamaecyparissus essential oil from Serbia, obtained via hydrodistillation, and includes a literature-based analysis of the existing profiles. Gas Chromatography–Mass Spectrometry (GC–MS) was employed for identifying the essential oil composition, while chemometric techniques like the genetic algorithm (GA), quantitative structure–retention relationship (QSRR) analysis, artificial neural network (ANN), and molecular descriptors were applied to ensure accurate and reliable results for authenticating the oil. Among the 47 identified compounds, oxygenated monoterpenes, especially artemisia ketone (36.11%), and oxygenated sesquiterpenes, notably vulgarone B (22.13%), were the primary constituents. Chemometric analysis proved effective in predicting the oil’s composition, and sensory evaluation revealed a herbal aroma with earthy, woody, and camphoraceous notes. A literature review highlighted the variability in oil composition due to geographical, environmental, and extraction factors, underscoring its chemical diversity, bioactivity, and potential applications. Full article
Show Figures

Figure 1

17 pages, 1123 KiB  
Review
Postharvest Flavor Quality Changes and Preservation Strategies for Peach Fruits: A Comprehensive Review
by Qiaoping Qin, Lili Wang, Qiankun Wang, Rongshang Wang, Chunxi Li, Yongjin Qiao and Hongru Liu
Plants 2025, 14(9), 1310; https://doi.org/10.3390/plants14091310 - 26 Apr 2025
Viewed by 1150
Abstract
Peach (Prunus persica (L.) Batsch) is valued for its flavor, nutrition, and economic importance, yet as a climacteric fruit, it undergoes rapid postharvest senescence due to respiratory surges and ethylene production, leading to flavor loss and reduced marketability. Recent advances in postharvest [...] Read more.
Peach (Prunus persica (L.) Batsch) is valued for its flavor, nutrition, and economic importance, yet as a climacteric fruit, it undergoes rapid postharvest senescence due to respiratory surges and ethylene production, leading to flavor loss and reduced marketability. Recent advances in postharvest physiology, including ethylene regulation, metabolic analysis, and advanced packaging, have improved preservation. Compared with traditional methods, emerging technologies, such as nanotechnology-based coatings and intelligent packaging systems, offer environmentally friendly and highly effective solutions but face high costs, technical barriers, and other constraints. This review examines changes in key flavor components—amino acids, phenolic compounds, sugars, organic acids, and volatile organic compounds (VOCs)—during ripening and senescence. It evaluates physical, chemical, and biotechnological preservation methods for maintaining quality. For instance, 1-MCP extends shelf life but may reduce aroma, underscoring the need for optimized protocols. Emerging trends, including biocontrol agents and smart packaging, provide a foundation for enhancing peach storage, transportation, and marketability. Full article
Show Figures

Figure 1

24 pages, 5739 KiB  
Article
Multifaceted Biological Activities of Culinary Herb and Spice Extracts: In Vitro and In Silico Simulation Insights into Inflammation-Related Targets
by Nance Hontman, Jéssica Gonçalves, José S. Câmara and Rosa Perestrelo
Foods 2025, 14(9), 1456; https://doi.org/10.3390/foods14091456 - 23 Apr 2025
Viewed by 668
Abstract
Culinary herbs and spices are valued worldwide for their flavor, aroma, and medicinal benefits. They encompass diverse bioactive metabolites, such as polyphenols and terpenoids, which contribute to plant defense and offer anticarcinogenic, anti-inflammatory, antioxidant, and cognitive-enhancing effects. This study aimed to establish the [...] Read more.
Culinary herbs and spices are valued worldwide for their flavor, aroma, and medicinal benefits. They encompass diverse bioactive metabolites, such as polyphenols and terpenoids, which contribute to plant defense and offer anticarcinogenic, anti-inflammatory, antioxidant, and cognitive-enhancing effects. This study aimed to establish the volatile fingerprint of culinary herbs (lemon verbena, chives, basil, sage, coriander, and parsley) and spices (curcuma, nutmeg, cumin, black pepper, Jamaica pepper, and juniper berry) using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME/GC-MS). The predominant volatile organic metabolites (VOMs) identified were subjected to in silico molecular docking simulations of anti-Alzheimer’s (e.g., acetylcholinesterase (AChE), butyrylcholinesterase (BChE)), antioxidants (e.g., monoamine oxidase B (MAO-B), inducible nitric oxide synthase (iNOS)), and anti-inflammatory receptors (e.g., 5-lipoxygenase (5-LOX), cyclooxygenase-2 (COX-2)). The culinary herb and spice extracts were also subjected to in vitro assays to evaluate their potential as antioxidant (DPPH, ABTS, and ORAC) and anti-inflammatory (% protein denaturation) agents. A total of 121 VOMs were identified in the culinary herbs and spices, with the predominant chemical families being monoterpenoids (48.3%), sesquiterpenoids (14.0%), esters (11.9%), and carbonyl compounds (8.8%). In silico molecular docking simulations revealed that cuminaldehyde, β-caryophyllene, γ-curcumene, germacrene D, and τ-cadinol exhibited the strongest inhibitory activities against the selected receptors. Among the extracts, Jamaica pepper showed the highest antioxidant and anti-inflammatory activities, while lemon verbena exhibited the lowest ones. These findings highlight the promising potential of the studied culinary herbs and spices in the modulation of inflammatory processes related to Alzheimer’s disease. However, further investigations, particularly clinical studies, are recommended to validate these results and explore their therapeutic applications. Full article
Show Figures

Figure 1

17 pages, 3443 KiB  
Article
Neem Oil (Azadirachta indica L.) Response Surface Methodology (RSM)-Optimized Nanoemulsions for Sensory Quality Preservation of Oreochromis niloticus Fillets
by Jamal Kazam, Khalid Javed Iqbal, Afshan Shafi, Usman Majeed and Maximilian Lackner
Biology 2025, 14(4), 400; https://doi.org/10.3390/biology14040400 - 10 Apr 2025
Viewed by 751
Abstract
Neem oil nanoemulsions (NO NEs) have gained attention as natural antibacterial agents due to toxicity concerns surrounding synthetic preservatives. This study aimed to prepare a response surface methodology (RSM)-optimized NO NE < 200 nm to achieve a stable dip solution to maintain the [...] Read more.
Neem oil nanoemulsions (NO NEs) have gained attention as natural antibacterial agents due to toxicity concerns surrounding synthetic preservatives. This study aimed to prepare a response surface methodology (RSM)-optimized NO NE < 200 nm to achieve a stable dip solution to maintain the sensory quality of Oreochromis niloticus fillets. The NO NE achieved a stable formulation with a particle size of 160.2 ± 0.04 nm on average. The polydispersity index (PDI) was 0.1 ± 0.05, and the zeta potential was found to be 18.2 ± 0.09 mV. Gas chromatography confirmed the presence of nimbiol, nimbandiol, 6-deacetyl nimbinene, and azadirachtin in NO after ultrasonic homogenization for 10 min (alternating between 30 s rest and 30 s work time). The NE had a spherical shape with a smooth surface, as was evident from transmission electron microscopy (TEM). Furthermore, NO:PM (neem oil–potassium metabisulphite) had an MIC (minimum inhibitory concentration) value of 150 ppm, compared to 210 ppm for the NO NE alone, against Staphylococcus aureus. Time–kill dynamics revealed the more effective control of S. aureus until 72 h with NO:PM. Moreover, DNA and protein leakage also increased from 0.145 ± 0.001 to 0.769 ± 0.002 OD (optical density) and from 0.142 ± 0.002 to 0.740 ± 0.001 OD, respectively, with the co-formulation of NO:PM. Conclusively, NO:PM inhibited S. aureus at a lower dose compared to the NO NE alone. Time–kill dynamics revealed complete inhibition of S. aureus in vitro for a period of 72 h. On the other hand, a proximate analysis of O. niloticus fillets showed no alteration in pH, no protein loss, and juiciness/moisture retention during 30 days of storage (4 °C). Sensory panelists reported that O. niloticus fillets treated with NE NO had improved color, flavor, juiciness, aroma, and overall quality. These results show that NE NO is a suitable green preservative for fish and possibly other meat-based products. Full article
(This article belongs to the Special Issue Microbial Contamination and Food Safety (Volume II))
Show Figures

Figure 1

15 pages, 455 KiB  
Review
Effect of Artificial Food Additives on Lung Health—An Overview
by Yousef Saad Aldabayan
Medicina 2025, 61(4), 684; https://doi.org/10.3390/medicina61040684 - 8 Apr 2025
Viewed by 1520
Abstract
This review focuses on the potential health risks of artificial food additives, especially their effects on lung health. Preservatives, synthetic colorants, and flavor enhancers, which are commonly used in processed foods, play roles in worsening respiratory diseases, such as asthma and chronic obstructive [...] Read more.
This review focuses on the potential health risks of artificial food additives, especially their effects on lung health. Preservatives, synthetic colorants, and flavor enhancers, which are commonly used in processed foods, play roles in worsening respiratory diseases, such as asthma and chronic obstructive pulmonary disease (COPD). These additives cause oxidative stress, systemic inflammation, and immune dysregulation, often through the gut-lung axis. The preservatives sodium nitrite and sulfites have the risk of causing bronchial hyper-responsiveness and allergic reactions. The synthetic colorant, Ponceau 4R, is also related to immune-mediated lung inflammation. Flavoring agents such as diacetyl contribute to occupational respiratory diseases like bronchiolitis obliterans. In animal models, prenatal exposure to additives, such as titanium dioxide (E171), might disrupt the development of respiratory neural networks, with long-term consequences. Ultra-processed foods (UPFs), which also contain a high concentration of additives, lead to systemic inflammation and impair lung function. Despite their wide usage, the use of these additives has become a warning sign due to their safety issue, particularly in sensitive people like children, pregnant women, and patients with pre-existing respiratory and chronic conditions. The review highlights the serious need for strict regulation and further research on the long-term effects of food additives on respiratory health. Policymakers should ban these food additives that are more harmful to human health. As an alternative to artificial additives, natural flavors and colors from fruits and vegetables, safe preservatives, and minimally processed ingredients can be used. Full article
(This article belongs to the Section Epidemiology & Public Health)
Show Figures

Figure 1

14 pages, 1544 KiB  
Article
Evaluation of the Effectiveness of Plant-Protein-Based Cleaning Agents in the Production of Industrial-Filtered Clarified Apple Juice
by Julianna Kereszturi, Csenge Béres, István Dalmadi and Mónika Máté
Appl. Sci. 2025, 15(6), 3415; https://doi.org/10.3390/app15063415 - 20 Mar 2025
Viewed by 597
Abstract
Apple (Malus domestica Borkh.) juice is a globally popular beverage that is valued for its pleasing flavor, wide availability, and health benefits, including support for cardiovascular health and antioxidant properties. A critical element of the production process is the clarification procedure, which [...] Read more.
Apple (Malus domestica Borkh.) juice is a globally popular beverage that is valued for its pleasing flavor, wide availability, and health benefits, including support for cardiovascular health and antioxidant properties. A critical element of the production process is the clarification procedure, which improves the product’s purity, visual appeal, and shelf stability by removing solids, colloids, and polyphenols. This study examines the efficacy of plant-based clarifiers, LittoFresh Liquid and FloaClair, in combination with three mineral agents—KlarSol30, GranuBent Pore-Tec, and Seporit Pore-Tec—on the quality of apple juice. The following analytical procedures were conducted: measurement of turbidity (NTU), color stability (ΔE*), transmittance at 440 nm, antioxidant capacity (FRAP), and total polyphenol content (TPC). The results showed that plant-based clarifiers were effective in reducing turbidity by up to 45% while improving transmittance levels by an average of 30% compared to untreated samples. Additionally, plant-based agents retained up to 20% more polyphenols and exhibited a 15% higher antioxidant capacity than traditional gelatin-based clarifiers. Full article
Show Figures

Figure 1

Back to TopTop