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18 pages, 6388 KiB  
Article
Spatial–Temporal Hotspot Management of Photovoltaic Modules Based on Fiber Bragg Grating Sensor Arrays
by Haotian Ding, Rui Guo, Huan Xing, Yu Chen, Jiajun He, Junxian Luo, Maojie Chen, Ye Chen, Shaochun Tang and Fei Xu
Sensors 2025, 25(15), 4879; https://doi.org/10.3390/s25154879 (registering DOI) - 7 Aug 2025
Abstract
Against the backdrop of an urgent energy crisis, solar energy has attracted sufficient attention as one of the most inexhaustible and friendly types of environmental energy. Faced with long service and harsh environment, the poor performance ratios of photovoltaic arrays and safety hazards [...] Read more.
Against the backdrop of an urgent energy crisis, solar energy has attracted sufficient attention as one of the most inexhaustible and friendly types of environmental energy. Faced with long service and harsh environment, the poor performance ratios of photovoltaic arrays and safety hazards are frequently boosted worldwide. In particular, the hot spot effect plays a vital role in weakening the power generation performance and reduces the lifetime of photovoltaic (PV) modules. Here, our research reports a spatial–temporal hot spot management system integrated with fiber Bragg grating (FBG) temperature sensor arrays and cooling hydrogels. Through finite element simulations and indoor experiments in laboratory conditions, a superior cooling effect of hydrogels and photoelectric conversion efficiency improvement have been demonstrated. On this basis, field tests were carried out in which the FBG arrays detected the surface temperature of the PV module first, and then a classifier based on an optimized artificial neural network (ANN) recognized hot spots with an accuracy of 99.1%. The implementation of cooling hydrogels as a feedback mechanism achieved a 7.7 °C reduction in temperature, resulting in a 5.6% enhancement in power generation efficiency. The proposed strategy offers valuable insights for conducting predictive maintenance of PV power plants in the case of hot spots. Full article
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20 pages, 1149 KiB  
Article
Assessment of Biomethane Potential from Waste Activated Sludge in Swine Wastewater Treatment and Its Co-Digestion with Swine Slurry, Water Lily, and Lotus
by Sartika Indah Amalia Sudiarto, Hong Lim Choi, Anriansyah Renggaman and Arumuganainar Suresh
AgriEngineering 2025, 7(8), 254; https://doi.org/10.3390/agriengineering7080254 (registering DOI) - 7 Aug 2025
Abstract
Waste activated sludge (WAS), a byproduct of livestock wastewater treatment, poses significant disposal challenges due to its low biodegradability and potential environmental impact. Anaerobic digestion (AD) offers a sustainable approach for methane recovery and sludge stabilization. This study evaluates the biomethane potential (BMP) [...] Read more.
Waste activated sludge (WAS), a byproduct of livestock wastewater treatment, poses significant disposal challenges due to its low biodegradability and potential environmental impact. Anaerobic digestion (AD) offers a sustainable approach for methane recovery and sludge stabilization. This study evaluates the biomethane potential (BMP) of WAS and its co-digestion with swine slurry (SS), water lily (Nymphaea spp.), and lotus (Nelumbo nucifera) shoot biomass to enhance methane yield. Batch BMP assays were conducted at substrate-to-inoculum (S/I) ratios of 1.0 and 0.5, with methane production kinetics analyzed using the modified Gompertz model. Mono-digestion of WAS yielded 259.35–460.88 NmL CH4/g VSadded, while co-digestion with SS, water lily, and lotus increased yields by 14.89%, 10.97%, and 16.89%, respectively, surpassing 500 NmL CH4/g VSadded. All co-digestion combinations exhibited synergistic effects (α > 1), enhancing methane production beyond individual substrate contributions. Lower S/I ratios improved methane yields and biodegradability, highlighting the role of inoculum availability. Co-digestion reduced the lag phase limitations of WAS and plant biomass, improving process efficiency. These findings demonstrate that co-digesting WAS with nutrient-rich co-substrates optimizes biogas production, supporting sustainable sludge management and renewable energy recovery in livestock wastewater treatment systems. Full article
(This article belongs to the Section Sustainable Bioresource and Bioprocess Engineering)
16 pages, 738 KiB  
Article
Modeling, Simulation, and Techno-Economic Assessment of a Spent Li-Ion Battery Recycling Plant
by Árpád Imre-Lucaci, Florica Imre-Lucaci and Szabolcs Fogarasi
Materials 2025, 18(15), 3715; https://doi.org/10.3390/ma18153715 - 7 Aug 2025
Abstract
The literature clearly indicates that both academia and industry are strongly committed to developing comprehensive processes for spent Li-ion battery (LIB) recycling. In this regard, the current study presents an original contribution by providing a quantitative assessment of a large-scale recycling plant designed [...] Read more.
The literature clearly indicates that both academia and industry are strongly committed to developing comprehensive processes for spent Li-ion battery (LIB) recycling. In this regard, the current study presents an original contribution by providing a quantitative assessment of a large-scale recycling plant designed for the treatment of completely spent LIBs. In addition to a concept of the basic process, this assessment also considers a case study of a thermal integration and CO2 capture subsystem. Process flow modeling software was used to evaluate the contribution of all process steps and equipment to overall energy consumption and to mass balance the data required for the technical assessment of the large-scale recycling plant. To underline the advantages and identify the optimal novel process concept, several key performance indicators were determined, such as recovery efficiency, specific energy/material consumption, and specific CO2 emissions. In addition, the economic potential of the recycling plants was evaluated for the defined case studies based on capital and O&M costs. The results indicate that, even with CO2 capture applied, the thermally integrated process with the combustion of hydrogen produced in the recycling plant remains the most promising large-scale configuration for spent LIB recycling. Full article
(This article belongs to the Special Issue Recycling and Electrode Materials of Lithium Batteries)
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32 pages, 5466 KiB  
Article
Comprehensive Energy and Economic Analysis of Selected Variants of a Large-Scale Photovoltaic Power Plant in a Temperate Climate
by Dennis Thom, Artur Bugała, Dorota Bugała and Wojciech Czekała
Energies 2025, 18(15), 4198; https://doi.org/10.3390/en18154198 - 7 Aug 2025
Abstract
In recent years, solar energy has emerged as one of the most advanced renewable energy sources, with its production capacity steadily growing. To maximize output and efficiency, choosing the right configuration for a specific location for these installations is crucial. This study uniquely [...] Read more.
In recent years, solar energy has emerged as one of the most advanced renewable energy sources, with its production capacity steadily growing. To maximize output and efficiency, choosing the right configuration for a specific location for these installations is crucial. This study uniquely integrates detailed multi-variant fixed-tilt PV system simulations with comprehensive economic evaluation under temperate climate conditions, addressing site-specific spatial constraints and grid integration considerations that have rarely been combined in previous works. In this paper, an energy and economic efficiency analysis for a photovoltaic power plant, located in central Poland, designed in eight variants (10°, 15°, 20°, 25°, 30° PV module inclination angle for a south orientation and 10°, 20°, 30° for an east–west orientation) for a limited building area of approximately 300,000 m2 was conducted. In PVSyst computer simulations, PVGIS-SARAH2 solar radiation data were used together with the most common data for describing the Polish local solar climate, called Typical Meteorological Year data (TMY). The most energy-efficient variants were found to be 20° S and 30° S, configurations with the highest surface production coefficient (249.49 and 272.68 kWh/m2) and unit production efficiency values (1123 and 1132 kWh/kW, respectively). These findings highlight potential efficiency gains of up to approximately 9% in surface production coefficient and financial returns exceeding 450% ROI, demonstrating significant economic benefits. In economic terms, the 15° S variant achieved the highest values of financial parameters, such as the return on investment (ROI) (453.2%), the value of the average annual share of profits in total revenues (56.93%), the shortest expected payback period (8.7 years), the value of the levelized cost of energy production (LCOE) (0.1 EUR/kWh), and one of the lowest costs of building 1 MWp of a photovoltaic farm (664,272.7 EUR/MWp). Among the tested variants of photovoltaic farms with an east–west geographical orientation, the most advantageous choice is the 10° EW arrangement. The results provide valuable insights for policymakers and investors aiming to optimize photovoltaic deployment in temperate climates, supporting the broader transition to renewable energy and alignment with national energy policy goals. Full article
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32 pages, 3396 KiB  
Article
Enhancing Smart and Zero-Carbon Cities Through a Hybrid CNN-LSTM Algorithm for Sustainable AI-Driven Solar Power Forecasting (SAI-SPF)
by Haytham Elmousalami, Felix Kin Peng Hui and Aljawharah A. Alnaser
Buildings 2025, 15(15), 2785; https://doi.org/10.3390/buildings15152785 - 6 Aug 2025
Abstract
The transition to smart, zero-carbon cities relies on advanced, sustainable energy solutions, with artificial intelligence (AI) playing a crucial role in optimizing renewable energy management. This study evaluates state-of-the-art AI models for solar power forecasting, emphasizing accuracy, reliability, and environmental sustainability. Using operational [...] Read more.
The transition to smart, zero-carbon cities relies on advanced, sustainable energy solutions, with artificial intelligence (AI) playing a crucial role in optimizing renewable energy management. This study evaluates state-of-the-art AI models for solar power forecasting, emphasizing accuracy, reliability, and environmental sustainability. Using operational data from Benban Solar Park in Egypt and Sakaka Solar Power Plant in Saudi Arabia, two of the world’s largest solar installations, the research highlights the effectiveness of hybrid AI techniques. The hybrid Convolutional Neural Network–Long Short-Term Memory (CNN-LSTM) model outperformed other models, achieving a Mean Absolute Percentage Error (MAPE) of 2.04%, Root Mean Square Error (RMSE) of 184, Mean Absolute Error (MAE) of 252, and R2 of 0.99 for Benban, and an MAPE of 2.00%, RMSE of 190, MAE of 255, and R2 of 0.98 for Sakaka. This model excels at capturing complex spatiotemporal patterns in solar data while maintaining low computational CO2 emissions, supporting sustainable AI practices. The findings demonstrate the potential of hybrid AI models to enhance the accuracy and sustainability of solar power forecasting, thereby contributing to efficient, resilient, and zero-carbon urban environments. This research provides valuable insights for policymakers and stakeholders aiming to advance smart energy infrastructure. Full article
(This article belongs to the Special Issue Intelligent Automation in Construction Management)
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30 pages, 3560 KiB  
Article
The Planning of Best Site Selection for Wind Energy in Indonesia: A Synergistic Approach Using Data Envelopment Analysis and Fuzzy Multi-Criteria Decision-Making
by Chia-Nan Wang, Yu-Chi Chung, Fajar Dwi Wibowo, Thanh-Tuan Dang and Ngoc-Ai-Thy Nguyen
Energies 2025, 18(15), 4176; https://doi.org/10.3390/en18154176 - 6 Aug 2025
Abstract
The objective of this study is to create an integrated and sustainability-centered framework to identify optimal locations for wind energy projects in Indonesia. This research employs a novel two-phase multi-criteria decision-making (MCDM) framework that combines the strengths of Data Envelopment Analysis (DEA), Fuzzy [...] Read more.
The objective of this study is to create an integrated and sustainability-centered framework to identify optimal locations for wind energy projects in Indonesia. This research employs a novel two-phase multi-criteria decision-making (MCDM) framework that combines the strengths of Data Envelopment Analysis (DEA), Fuzzy Analytic Hierarchy Process (FAHP), and Fuzzy Combined Compromise Solution (F-CoCoSo). Initially, DEA is utilized to pinpoint the most promising sites based on a variety of quantitative factors. Subsequently, these sites are evaluated against qualitative criteria such as technical, economic, environmental, and socio-political considerations using FAHP for criteria weighting and F-CoCoSo for ranking the sites. Comprehensive sensitivity analysis of the criteria weights and a comparative assessment of methodologies substantiate the robustness of the proposed framework. The results converge on consistent rankings across methods, highlighting the effectiveness of the integrated approach. Notably, the results consistently identify Lampung, Aceh, and Riau as the top-ranked provinces, showcasing their strategic suitability for wind plant development. This framework provides a systematic approach for enhancing resource efficiency and strategic planning in Indonesia’s renewable energy sector. Full article
(This article belongs to the Special Issue Progress and Challenges in Wind Farm Optimization)
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22 pages, 3208 KiB  
Article
Upstream Microplastic Removal in Industrial Wastewater: A Pilot Study on Agglomeration-Fixation-Reaction Based Treatment for Water Reuse and Waste Recovery
by Anika Korzin, Michael Toni Sturm, Erika Myers, Dennis Schober, Pieter Ronsse and Katrin Schuhen
Clean Technol. 2025, 7(3), 67; https://doi.org/10.3390/cleantechnol7030067 - 6 Aug 2025
Abstract
This pilot study investigated an automated pilot plant for removing microplastics (MPs) from industrial wastewater that are generated during packaging production. MP removal is based on organosilane-induced agglomeration-fixation (clump & skim technology) followed by separation. The wastewater had high MP loads (1725 ± [...] Read more.
This pilot study investigated an automated pilot plant for removing microplastics (MPs) from industrial wastewater that are generated during packaging production. MP removal is based on organosilane-induced agglomeration-fixation (clump & skim technology) followed by separation. The wastewater had high MP loads (1725 ± 377 mg/L; 673 ± 183 million particles/L) and an average COD of 7570 ± 1339 mg/L. Over 25 continuous test runs, the system achieved consistent performance, removing an average of 97.4% of MPs by mass and 99.1% by particle count, while reducing the COD by 78.8%. Projected over a year, this equates to preventing 1.7 tons of MPs and 6 tons of COD from entering the sewage system. Turbidity and photometric TSS measurements proved useful for process control. The approach supports water reuse—with water savings up to 80%—and allows recovery of agglomerates for recycling and reuse. Targeting pollutant removal upstream at the source provides multiple financial and environmental benefits, including lower overall energy demands, higher removal efficiencies, and process water reuse. This provides financial and environmental incentives for industries to implement sustainable solutions for pollutants and microplastic removal. Full article
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23 pages, 3036 KiB  
Article
Research on the Synergistic Mechanism Design of Electricity-CET-TGC Markets and Transaction Strategies for Multiple Entities
by Zhenjiang Shi, Mengmeng Zhang, Lei An, Yan Lu, Daoshun Zha, Lili Liu and Tiantian Feng
Sustainability 2025, 17(15), 7130; https://doi.org/10.3390/su17157130 - 6 Aug 2025
Abstract
In the context of the global response to climate change and the active promotion of energy transformation, a number of low-carbon policies coupled with the development of synergies to help power system transformation is an important initiative. However, the insufficient articulation of the [...] Read more.
In the context of the global response to climate change and the active promotion of energy transformation, a number of low-carbon policies coupled with the development of synergies to help power system transformation is an important initiative. However, the insufficient articulation of the green power market, tradable green certificate (TGC) market, and carbon emission trading (CET) mechanism, and the ambiguous policy boundaries affect the trading decisions made by its market participants. Therefore, this paper systematically analyses the composition of the main players in the electricity-CET-TGC markets and their relationship with each other, and designs the synergistic mechanism of the electricity-CET-TGC markets, based on which, it constructs the optimal profit model of the thermal power plant operators, renewable energy manufacturers, power grid enterprises, power users and load aggregators under the electricity-CET-TGC markets synergy, and analyses the behavioural decision-making of the main players in the electricity-CET-TGC markets as well as the electric power system to optimise the trading strategy of each player. The results of the study show that: (1) The synergistic mechanism of electricity-CET-TGC markets can increase the proportion of green power grid-connected in the new type of power system. (2) In the selection of different environmental rights and benefits products, the direct participation of green power in the market-oriented trading is the main way, followed by applying for conversion of green power into China certified emission reduction (CCER). (3) The development of independent energy storage technology can produce greater economic and environmental benefits. This study provides policy support to promote the synergistic development of the electricity-CET-TGC markets and assist the low-carbon transformation of the power industry. Full article
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23 pages, 331 KiB  
Article
Revisiting the Nexus Between Energy Consumption, Economic Growth, and CO2 Emissions in India and China: Insights from the Long Short-Term Memory (LSTM) Model
by Bartosz Jóźwik, Siba Prasada Panda, Aruna Kumar Dash, Pritish Kumar Sahu and Robert Szwed
Energies 2025, 18(15), 4167; https://doi.org/10.3390/en18154167 - 6 Aug 2025
Abstract
Understanding how energy use and economic activity shape carbon emissions is pivotal for achieving global climate targets. This study quantifies the dynamic nexus between disaggregated energy consumption, economic growth, and CO2 emissions in India and China—two economies that together account for more [...] Read more.
Understanding how energy use and economic activity shape carbon emissions is pivotal for achieving global climate targets. This study quantifies the dynamic nexus between disaggregated energy consumption, economic growth, and CO2 emissions in India and China—two economies that together account for more than one-third of global emissions. Using annual data from 1990 to 2021, we implement Long Short-Term Memory (LSTM) neural networks, which outperform traditional linear models in capturing nonlinearities and lagged effects. The dataset is split into training (1990–2013) and testing (2014–2021) intervals to ensure rigorous out-of-sample validation. Results reveal stark national differences. For India, coal, natural gas consumption, and economic growth are the strongest positive drivers of emissions, whereas renewable energy exerts a significant mitigating effect, and nuclear energy is negligible. In China, emissions are dominated by coal and petroleum use and by economic growth, while renewable and nuclear sources show weak, inconsistent impacts. We recommend retrofitting India’s coal- and gas-plants with carbon capture and storage, doubling clean-tech subsidies, and tripling annual solar-plus-storage auctions to displace fossil baseload. For China, priorities include ultra-supercritical upgrades with carbon capture, utilisation, and storage, green-bond-financed solar–wind buildouts, grid-scale storage deployments, and hydrogen-electric freight corridors. These data-driven pathways simultaneously cut flagship emitters, decouple GDP from carbon, provide replicable models for global net-zero research, and advance climate-resilient economic growth worldwide. Full article
(This article belongs to the Special Issue Policy and Economic Analysis of Energy Systems)
24 pages, 2540 KiB  
Article
Classification Framework for Hydrological Resources for Sustainable Hydrogen Production with a Predictive Algorithm for Optimization
by Mónica Álvarez-Manso, Gabriel Búrdalo-Salcedo and María Fernández-Raga
Hydrogen 2025, 6(3), 54; https://doi.org/10.3390/hydrogen6030054 - 6 Aug 2025
Abstract
Given the urgent need to decarbonize the global energy system, green hydrogen has emerged as a key alternative in the transition to renewables. However, its production via electrolysis demands high water quality and raises environmental concerns, particularly regarding reject water discharge. This study [...] Read more.
Given the urgent need to decarbonize the global energy system, green hydrogen has emerged as a key alternative in the transition to renewables. However, its production via electrolysis demands high water quality and raises environmental concerns, particularly regarding reject water discharge. This study employs an experimental and analytical approach to define optimal water characteristics for electrolysis, focusing on conductivity as a key parameter. A pilot water treatment plant with reverse osmosis and electrodeionization (EDI) was designed to simulate industrial-scale pretreatment. Twenty water samples from diverse natural sources (surface and groundwater) were tested, selected for geographical and geological variability. A predictive algorithm was developed and validated to estimate useful versus reject water based on input quality. Three conductivity-based categories were defined: optimal (0–410 µS/cm), moderate (411–900 µS/cm), and restricted (>900 µS/cm). Results show that water quality significantly affects process efficiency, energy use, waste generation, and operating costs. This work offers a technical and regulatory framework for assessing potential sites for green hydrogen plants, recommending avoidance of high-conductivity sources. It also underscores the current regulatory gap regarding reject water treatment, stressing the need for clear environmental guidelines to ensure project sustainability. Full article
(This article belongs to the Special Issue Advances in Hydrogen Production, Storage, and Utilization)
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21 pages, 3334 KiB  
Article
Market Research on Waste Biomass Material for Combined Energy Production in Bulgaria: A Path Toward Enhanced Energy Efficiency
by Penka Zlateva, Angel Terziev, Mariana Murzova, Nevena Mileva and Momchil Vassilev
Energies 2025, 18(15), 4153; https://doi.org/10.3390/en18154153 - 5 Aug 2025
Abstract
Using waste biomass as a raw material for the combined production of electricity and heat offers corresponding energy, economic, environmental and resource efficiency benefits. The study examines both the performance of a system for combined energy production based on the Organic Rankine Cycle [...] Read more.
Using waste biomass as a raw material for the combined production of electricity and heat offers corresponding energy, economic, environmental and resource efficiency benefits. The study examines both the performance of a system for combined energy production based on the Organic Rankine Cycle (ORC) utilizing wood biomass and the market interest in its deployment within Bulgaria. Its objective is to propose a technically and economically viable solution for the recovery of waste biomass through the combined production of electricity and heat while simultaneously assessing the readiness of industrial and municipal sectors to adopt such systems. The cogeneration plant incorporates an ORC module enhanced with three additional economizers that capture residual heat from flue gases. Operating on 2 t/h of biomass, the system delivers 1156 kW of electric power and 3660 kW of thermal energy, recovering an additional 2664 kW of heat. The overall energy efficiency reaches 85%, with projected annual revenues exceeding EUR 600,000 and a reduction in carbon dioxide emissions of over 5800 t/yr. These indicators can be achieved through optimal installation and operation. When operating at a reduced load, however, the specific fuel consumption increases and the overall efficiency of the installation decreases. The marketing survey results indicate that 75% of respondents express interest in adopting such technologies, contingent upon the availability of financial incentives. The strongest demand is observed for systems with capacities up to 1000 kW. However, significant barriers remain, including high initial investment costs and uneven access to raw materials. The findings confirm that the developed system offers a technologically robust, environmentally efficient and market-relevant solution, aligned with the goals of energy independence, sustainability and the transition to a low-carbon economy. Full article
(This article belongs to the Section B: Energy and Environment)
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22 pages, 6187 KiB  
Article
Device Modeling Method for the Entire Process of Energy-Saving Retrofit of a Refrigeration Plant
by Xuanru Xu, Lun Zhang, Jun Chen, Qingbin Lin and Junjie Chen
Energies 2025, 18(15), 4147; https://doi.org/10.3390/en18154147 - 5 Aug 2025
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Abstract
With the increasing awareness of energy consumption issues, there has been a growing emphasis on energy-saving retrofits for central air-conditioning systems that constitute a significant proportion of energy consumption in buildings. Efficient energy utilization can be achieved by optimizing the modeling of the [...] Read more.
With the increasing awareness of energy consumption issues, there has been a growing emphasis on energy-saving retrofits for central air-conditioning systems that constitute a significant proportion of energy consumption in buildings. Efficient energy utilization can be achieved by optimizing the modeling of the equipment within the chiller plants of central air-conditioning systems. Traditional modeling approaches have been static and have focused on modeling within narrow time frames when a certain amount of equipment operating data has accumulated, thus prioritizing the precision of the model itself while overlooking the fact that energy-saving retrofits are a long-term process. This study proposes a modeling scheme for the equipment within chiller plants throughout the energy-saving retrofit process. Based on the differences in the amount of available operating data for the equipment and the progress of retrofit implementation, the retrofit process was divided into three stages, each employing different modeling techniques and ensuring smooth transitions between the stages. The equipment within the chiller plants is categorized into two types based on the clarity of their operating characteristics, and two modeling schemes are proposed accordingly. Based on the proposed modeling scheme, chillers and chilled-water pumps were selected to represent the two types of equipment. Real operating data from actual retrofit projects was used to model the equipment and evaluate the accuracy of the model predictions. The results indicate that the models established by the proposed modeling scheme exhibit good accuracy at each stage of the retrofit, with the coefficients of variation (CV) remaining below 6.88%. Furthermore, the prediction accuracy improved as the retrofitting process progressed. The modeling scheme performs better on equipment with simpler and clearer operating characteristics, with a CV as low as 0.67% during normal operation stages. This underscores the potential application of the proposed modeling scheme throughout the energy-saving retrofit process and provides a model foundation for the subsequent optimization of the refrigeration system. Full article
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20 pages, 4055 KiB  
Article
Biphasic Salt Effects on Lycium ruthenicum Germination and Growth Linked to Carbon Fixation and Photosynthesis Gene Expression
by Xinmeng Qiao, Ruyuan Wang, Lanying Liu, Boya Cui, Xinrui Zhao, Min Yin, Pirui Li, Xu Feng and Yu Shan
Int. J. Mol. Sci. 2025, 26(15), 7537; https://doi.org/10.3390/ijms26157537 - 4 Aug 2025
Viewed by 166
Abstract
Since the onset of industrialization, the safety of arable land has become a pressing global concern, with soil salinization emerging as a critical threat to agricultural productivity and food security. To address this challenge, the cultivation of economically valuable salt-tolerant plants has been [...] Read more.
Since the onset of industrialization, the safety of arable land has become a pressing global concern, with soil salinization emerging as a critical threat to agricultural productivity and food security. To address this challenge, the cultivation of economically valuable salt-tolerant plants has been proposed as a viable strategy. In the study, we investigated the physiological and molecular responses of Lycium ruthenicum Murr. to varying NaCl concentrations. Results revealed a concentration-dependent dual effect: low NaCl levels significantly promoted seed germination, while high concentrations exerted strong inhibitory effects. To elucidate the mechanisms underlying these divergent responses, a combined analysis of metabolomics and transcriptomics was applied to identify key metabolic pathways and genes. Notably, salt stress enhanced photosynthetic efficiency through coordinated modulation of ribulose 5-phosphate and erythrose-4-phosphate levels, coupled with the upregulation of critical genes encoding RPIA (Ribose 5-phosphate isomerase A) and RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase). Under low salt stress, L. ruthenicum maintained intact cellular membrane structures and minimized oxidative damage, thereby supporting germination and early growth. In contrast, high salinity severely disrupted PS I (Photosynthesis system I) functionality, blocking energy flow into this pathway while simultaneously inducing membrane lipid peroxidation and triggering pronounced cellular degradation. This ultimately suppressed seed germination rates and impaired root elongation. These findings suggested a mechanistic framework for understanding L. ruthenicum adaptation under salt stress and pointed out a new way for breeding salt-tolerant crops and understanding the mechanism. Full article
(This article belongs to the Section Molecular Biology)
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23 pages, 2655 KiB  
Article
Ribosomal RNA-Specific Antisense DNA and Double-Stranded DNA Trigger rRNA Biogenesis and Insecticidal Effects on the Insect Pest Coccus hesperidum
by Vol Oberemok, Nikita Gal’chinsky, Ilya Novikov, Alexander Sharmagiy, Ekaterina Yatskova, Ekaterina Laikova and Yuri Plugatar
Int. J. Mol. Sci. 2025, 26(15), 7530; https://doi.org/10.3390/ijms26157530 - 4 Aug 2025
Viewed by 200
Abstract
Contact unmodified antisense DNA biotechnology (CUADb), developed in 2008, employs short antisense DNA oligonucleotides (oligos) as a novel approach to insect pest control. These oligonucleotide-based insecticides target pest mature rRNAs and/or pre-rRNAs and have demonstrated high insecticidal efficacy, particularly against sap-feeding insect pests, [...] Read more.
Contact unmodified antisense DNA biotechnology (CUADb), developed in 2008, employs short antisense DNA oligonucleotides (oligos) as a novel approach to insect pest control. These oligonucleotide-based insecticides target pest mature rRNAs and/or pre-rRNAs and have demonstrated high insecticidal efficacy, particularly against sap-feeding insect pests, which are key vectors of plant DNA viruses and among the most economically damaging herbivorous insects. To further explore the potential of CUADb, this study evaluated the insecticidal efficacy of short 11-mer antisense DNA oligos against Coccus hesperidum, in comparison with long 56-mer single-stranded and double-stranded DNA sequences. The short oligos exhibited higher insecticidal activity. By day 9, the highest mortality rate (97.66 ± 4.04%) was recorded in the Coccus-11 group, while the most effective long sequence was the double-stranded DNA in the dsCoccus-56 group (77.09 ± 6.24%). This study also describes the architecture of the DNA containment (DNAc) mechanism, highlighting the intricate interactions between rRNAs and various types of DNA oligos. During DNAc, the Coccus-11 treatment induced enhanced ribosome biogenesis and ATP production through a metabolic shift from carbohydrates to lipid-based energy synthesis. However, this ultimately led to a ‘kinase disaster’ due to widespread kinase downregulation resulting from insufficient ATP levels. All DNA oligos with high or moderate complementarity to target rRNA initiated hypercompensation, but subsequent substantial rRNA degradation and insect mortality occurred only when the oligo sequence perfectly matched the rRNA. Both short and long oligonucleotide insecticide treatments led to a 3.75–4.25-fold decrease in rRNA levels following hypercompensation, which was likely mediated by a DNA-guided rRNase, such as RNase H1, while crucial enzymes of RNAi (DICER1, Argonaute 2, and DROSHA) were downregulated, indicating fundamental difference in molecular mechanisms of DNAc and RNAi. Consistently, significant upregulation of RNase H1 was detected in the Coccus-11 treatment group. In contrast, treatment with random DNA oligos resulted in only a 2–3-fold rRNA decrease, consistent with the normal rRNA half-life maintained by general ribonucleases. These findings reveal a fundamental new mechanism of rRNA regulation via complementary binding between exogenous unmodified antisense DNA and cellular rRNA. From a practical perspective, this minimalist approach, applying short antisense DNA dissolved in water, offers an effective, eco-friendly and innovative solution for managing sternorrhynchans and other insect pests. The results introduce a promising new concept in crop protection: DNA-programmable insect pest control. Full article
(This article belongs to the Special Issue New Insights into Plant and Insect Interactions (Second Edition))
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21 pages, 3452 KiB  
Article
Features of Ash and Slag Formation During Incomplete Combustion of Coal from the Karazhyra Deposit in Small- and Medium-Scale Power Plants
by Natalya Seraya, Vadim Litvinov, Gulzhan Daumova, Maksat Shaikhov, Raigul Ramazanova and Roza Aubakirova
Processes 2025, 13(8), 2467; https://doi.org/10.3390/pr13082467 - 4 Aug 2025
Viewed by 101
Abstract
The study presents a comprehensive assessment of the combustion efficiency of low-grade coal from the Karazhyra deposit in small- and medium-capacity boiler units of the energy workshops operated by Vostokenergo LLP (East Kazakhstan Region, Kazakhstan). It was found that the average annual thermal [...] Read more.
The study presents a comprehensive assessment of the combustion efficiency of low-grade coal from the Karazhyra deposit in small- and medium-capacity boiler units of the energy workshops operated by Vostokenergo LLP (East Kazakhstan Region, Kazakhstan). It was found that the average annual thermal energy output amounts to 2,387,348.85 GJ with a coal consumption of 164,328.5 tons. Based on operational data from 2016 to 2017, the average thermal efficiency (boiler efficiency) was 66.03%, with a maximum value of 75% recorded at the Zhezkent energy workshop. The average lower heating value (LHV) of the coal was 19.41 MJ/kg, which is below the design value of 20.52 MJ/kg, indicating the use of coal with reduced energy characteristics and elevated ash content (21.4%). The unburned carbon content in the ash and slag waste (ASW) was determined to be between 14 and 35%, indicating incomplete combustion. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analyses revealed the presence of microspheres, porous granules, and coal residues, with silicon and aluminum oxides dominating the composition (up to 70.49%). Differences in the pollutant potential of ash from different boiler units were identified. Recommendations were substantiated regarding the adjustment of the air–fuel regime, modernization of combustion control systems, and utilization of ASW. The results may be used to develop measures aimed at improving the energy efficiency and environmental safety of coal-fired boiler plants. Full article
(This article belongs to the Section Chemical Processes and Systems)
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