334 Results Found

The alteration of tumorigenic pathways leading to cancer is a degenerative disease process typically involving inactivation of tumor suppressor proteins and hyperactivation of oncogenes. One such oncogenic protein product is the murine double-minute...

The study aim was to apply murine double minute 2 (MDM2)-siRNA to a biodegradable siRNA delivery vector, ternary complex, for treating colorectal cancer peritoneal dissemination. The ternary complex containing MDM2-siRNA (MDM2-siRNA complex) was cons...

Thymic stromal lymphopoietin (TSLP) is crucial for Th2-mediated inflammation. Sepsis is a serious systemic inflammatory reaction with organ dysfunction by infection. However, the function of TSLP during sepsis is poorly understood. Thus, we investiga...

The disordered p53 transactivation domain (p53TAD) contains specific levels of transient helical secondary structure that are necessary for its binding to the negative regulators, mouse double minute 2 (Mdm2) and MdmX. The interactions of p53 with Md...

Several indole derivatives have been disclosed by our research groups that have been collaborating for nearly 25 years. The results of our investigations led to a variety of molecules binding selectively to different pharmacological targets, specific...

Murine double minute 2 (MDM2) is involved in various cancers and is an attractive target. The RING domain of MDM2 has been discussed as an alternative target to stabilize p53. Designing drugs to target the RING domain of MDM2 is an alternative approa...

Oncogene amplification is closely linked to the pathogenesis of a broad spectrum of human malignant tumors. The amplified genes localize either to the extrachromosomal circular DNA, which has been referred to as cytogenetically visible double minutes...

Proteolysis-targeting chimeras (PROTACs) are molecules that selectively degrade a protein of interest (POI). The incorporation of ligands that recruit mouse double minute 2 (MDM2) into PROTACs, forming the so-called MDM2-based PROTACs, has shown prom...

A novel function of retinoid X receptor beta (RXRβ) in endothelial cells has been reported by us during the formation of atherosclerosis. Here, we extended the study to explore the cellular mechanisms of RXRβ protein stability regulation. I...

Ferroptosis, an iron-dependent cell death driven by lipid peroxidation, is regulated by key mediators including glutathione peroxidase 4 (GPX4) and nuclear factor erythroid 2-related factor 2 (Nrf2). Kaposi’s sarcoma-associated herpesvirus (KSH...

The tumor suppressor protein p53 is inactivated in a large variety of cancer cells. Cellular p53 inhibitors like the mouse double minute 2 homolog (MDM2) commonly suppress the p53 function in acute myeloid leukemia (AML). Moreover, fms like tyrosine...

Most low-penetrance genetic risk factors for cancer are located in noncoding regions, presumably altering the regulation of neighboring genes. The poorly characterized Indel polymorphism rs150550023 (rs3730485; del1518) in the promoter of MDM2 (human...

Focal amplifications (FAs) are crucial in cancer research due to their significant diagnostic, prognostic, and therapeutic implications. FAs manifest in various forms, such as episomes, double minute chromosomes, and homogeneously staining regions, a...

As the most common gene mutation found in cancers, p53 mutations are detected in up to 96% of high-grade serous ovarian carcinoma (HGSOC). Meanwhile, mutant p53 overexpression is known to drive oncogenic phenotypes in cancer patients and to sustain t...

Genome-wide association studies have reported link between SNPs and risk of breast cancer. This study investigated the association of the selected gene variants by predicting them as possible target genes. Molecular technique advances with the availa...

Mutation in p53 is the most frequent event in cancer development and a leading cause of cancer therapy resistance due to evasion of the apoptosis cascade. Beyond chemotherapies and radiation therapies, growing evidence indicates that p53-mutant tumor...

The p53 pathway is inactivated in the majority of human cancers. Although this perturbation frequently occurs through the mutation or deletion of p53 itself, there are other mechanisms that can attenuate the pathway and contribute to tumorigenesis. F...

This review presents the last decade of studies on the synthesis of various types of small-molecule inhibitors of the p53– Mouse double minute 2 homolog (MDM2) protein–protein interaction. The main focus is placed on synthetic approaches to such mole...

Reliable prediction of binding affinities for ligand-receptor complex has been the primary goal of a structure-based drug design process. In this respect, alchemical methods are evolving as a popular choice to predict the binding affinities for biomo...

Background: The purpose of this investigation was to identify the onset of the thermic effect of feeding (TEF) after ingestion of a high carbohydrate (CHO) and a high protein (PRO) 1255 kJ (300 kcal) drink. Methods: Resting metabolic rate (RMR) and T...

It is recommended that endurance athletes consume carbohydrate (CHO) supplements, providing 6–8% CHO concentration, during exercise > 60 minutes to improve athletic performance. Recently research has compared carbohydrate-protein (CHO-P) supplemen...

The rating of perceived exertion (RPE) indicates the feeling of fatigue. However, hypoxia worsens the condition and can worsen RPE. We evaluated whether carbohydrate and glutamine supplementation alters RPE and physiological markers in running at 70%...

In the current pilot study, we attempt to find out how double neurofeedback influences functional hemispheric asymmetry and activity. We examined 30 healthy participants (8 males; 22 females, mean age = 29; SD = 8). To measure functional hemispheric...

Background: The purpose of this study was to examine the cardiovascular effects of two lowlevels of caffeine ingestion in non habitual caffeine users at various submaximal and maximal exercise intensities. Methods: Nine male subjects (19–25 yr; 83.3...

Background/Objectives: Long non-coding RNAs (lncRNAs) are integral to the regulation of viral tumorigenesis. We have previously identified that the chicken lncRNA-803, which responds to Marek’s disease virus (MDV), inhibits apoptosis in the chi...

In view of the upcoming missions to obtain resources from the lunar surface, it is essential to have highly-accurate navigation systems to locate surface vehicles in shadowed regions. In response, we propose a dual-satellite lunar navigation system t...

The precise point positioning (PPP) technique generally takes tens of minutes to converge, severely limiting its use. This longer convergence time is mainly due to the slower variation of satellite geometry in space and the stronger correlation of un...

The intensity of large muscle mass exercise declines at altitude due to reduced oxygen delivery to active muscles. The purpose of this investigation was to determine if the greater limb blood flow during single-leg cycling prevents the reduction in t...

Background: Paraxanthine, when ingested in doses of 50–200 mg in the fasted state, enhances cognitive performance, including memory, reaction time, and attention, while suppressing hunger. However, unlike caffeine, paraxanthines effects on energy, fo...

Gait variability (GV), which is a variable for predicting mobility issues and risk of falling in elderly people, is defined as the fluctuation in spatiotemporal characteristics from one step to the next in walking. The goal of this study was to analy...

While pricing policy has emerged as a critical demand-side lever for decarbonizing mobility, its bidirectional effects on modal shift remain unexplored. Dynamic pricing in high-speed rail (HSR) creates a double-edged environmental outcome: advance di...

Deferoxamine (DFO), a hydroxamic siderophore with a high affinity for Fe(III), is immobilized as a functionalized self-assembled monolayer of a thiol (SAM) on the gold surface of a screen-printed cell to develop a voltammetric sensor for iron(III). T...

A novel optical label-free bio-sensing platform based on a new class of resonances supported in a photonic crystal metasurface is reported herein. Molecular binding is detected as a shift in the resonant wavelength of the bound states in the continuu...

A series of sulfamide and triazole benzodiazepines were obtained with the principle of bioisosterism. The p53-murine double minute 2 (MDM2) inhibitory activity and in vitro antitumor activity were evaluated. Most of the novel benzodiazepines exhibite...

This study proposes a non-invasive dual respiratory monitoring system for intensive care units (ICU), based on a triaxial MEMS accelerometer ADXL345 attached to the thoracic cage and a nasal NTC thermistor, interfaced with an Arduino Mega 2560 microc...

The aim of this study was to determine whether a rowing training program could improve cardiac function in women (n = 28) with the mean age of 52.30 ± 3.78 years who survived breast cancer (stage 1: 4.55%; 2: 36.36%; 3: 54.54%; and 4: 4.55%) diagnose...

Community Energy Markets (CEMs) enable trading opportunities between participants in a community to achieve savings and profits. However, the market design and the behaviour of participants are key factors that determine the success of such markets....

We have recently demonstrated that oral intake of glycine propionyl-L-carnitine (GPLC) increases plasma nitrate/nitrite (NOx), a surrogate measure of nitric oxide production. However, these findings were observed at rest, and in previously sedentary...

Little is known about the mutational processes that shape the genetic landscape of gliomas. Numerous mutational processes leave marks on the genome in the form of mutations, copy number alterations, rearrangements or their combinations. To explore gl...

Background: Previous research has indicated that phosphatidylserine (PS) supplementation has the potential to attenuate the serum cortisol response to acute exercise stress. Equivocal findings suggest that this effect might be dose dependent. This st...

Background: This study investigated the effects of caffeinated chewing gum on fatigue index and 400-meter performance in trained sprinters. Methods: Nineteen participants (age: 20.9 ± 1.0 years; height: 175.6 ± 4.9 cm; mass: 66.5 ± 5.6 kg; training a...

This research examines the flexural fatigue response of 3D-printed foot orthoses produced from various thermoplastic polyurethane (TPU) filaments, including Filaflex 60A, Filaflex 70A, Filaflex 82A, PolyFlex 90A, and varioShore. To subject the insole...

Murine Double Minute Clone 2, located at 12q15, is an oncogene that codes for an oncoprotein of which the association with p53 was discovered 30 years ago. The most important function of MDM2 is to control p53 activity; it is in fact the best documen...

The study of protein–protein interactions is of great interest. Several early studies focused on the murine double minute 2 (Mdm2)–tumor suppressor protein p53 interactions. However, the effect of plasma treatment on Mdm2 and p53 is still absent from...

Background: Caffeine based energy shot products accounted for $1.3 billion in sales in 2011. Caffeine has been shown to confer numerous benefits during exercise and is oftentimes combined with ingredients such as carbohydrates and taurine in the hope...

A layer of silver nanoplates, specifically synthesized with the desired localized surface plasmon resonance (LSPR) features, was grafted on amino-functionalized bulk glass surfaces to impart a double antibacterial action: (i) the well-known, long-ter...

Background: Phosphatidylserine (PS) is a phospholipid found in cell membranes of most animals and plants. PS has been shown to reduce stress and increase performance in runners, cyclists and golfers. The purpose of this study was to investigate the e...

Thermal insulation is a basic property for describing a set of clothing and consists of the thermal resistance of the individual layers of clothing (which depends on the material used and its structure) and also takes into account the air gaps betwee...

In this study, we analyzed a large number of vertical sounding ionograms, obtained by the mid-latitude Cyclone ionosonde (55.85° N; 48.8° E) of Kazan (Volga Region) Federal University, which operates in a rapid-run mode of ionograms (1 ionogram per m...

Ladle metallurgy serves as a crucial component of the steelmaking industry, where it plays a pivotal role in manipulating the molten steel to exercise precise control over its composition and properties. Turbulence in ladle metallurgy influences vari...