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19 pages, 9214 KiB  
Article
Tribological Performance of Direct Metal Laser Sintered 20MnCr5 Tool Steel Countersamples Designed for Sheet Metal Forming Applications
by Krzysztof Żaba, Marcin Madej, Beata Leszczyńska-Madej, Tomasz Trzepieciński and Ryszard Sitek
Appl. Sci. 2025, 15(15), 8711; https://doi.org/10.3390/app15158711 (registering DOI) - 6 Aug 2025
Abstract
This article presents the results of the tribological performance of 20MnCr5 (1.7147) tool steel countersamples produced by Direct Metal Laser Sintering (DMLS), as a potential material for inserts or working layers of sheet metal forming tools. Tribological tests were performed using a roller-block [...] Read more.
This article presents the results of the tribological performance of 20MnCr5 (1.7147) tool steel countersamples produced by Direct Metal Laser Sintering (DMLS), as a potential material for inserts or working layers of sheet metal forming tools. Tribological tests were performed using a roller-block tribotester. The samples were sheet metals made of materials with significantly different properties: Inconel 625, titanium-stabilised stainless steel 321, EN AW-6061 T0 aluminium alloy, and pure copper. The samples and countersamples were analysed in terms of their wear resistance, coefficient of friction (COF), changes in friction force during testing, and surface morphology after tribological contact under dry friction conditions. The tests were performed on DMLSed countersamples in the as-received state. The largest gain of countersample mass was observed for the 20MnCr5/EN AW-6061 T0 friction pair. The sample mass loss in this combination was also the largest, amounting to 19.96% of the initial mass. On the other hand, in the 20MnCr5/Inconel 625 friction pair, no significant changes in the mass of materials were recorded. For the Inconel 625 sample, a mass loss of 0.04% was observed. The basic wear mechanism of the samples was identified as abrasive wear. The highest friction forces were observed in the 20MnCr5/Cu friction pair (COF = 0.913) and 20MnCr5/EN AW-6061 T0 friction pair (COF = 1.234). The other two samples (Inconel 625, 321 steel) showed a very stable value of the friction force during the roller-block test resulting in a COF between 0.194 and 0.213. Based on the changes in friction force, COFs, and mass changes in friction pair components during wear tests, it can be concluded that potential tools in the form of inserts or working layers manufactured using 3D printing technology, the DMLS method, without additional surface treatment can be successfully used for forming sheets of 321 steel and Inconel 625. Full article
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16 pages, 3291 KiB  
Article
A Discrete Element Model for Characterizing Soil-Cotton Seeding Equipment Interactions Using the JKR and Bonding Contact Models
by Xuyang Ran, Long Wang, Jianfei Xing, Lu Shi, Dewei Wang, Wensong Guo and Xufeng Wang
Agriculture 2025, 15(15), 1693; https://doi.org/10.3390/agriculture15151693 - 5 Aug 2025
Abstract
Due to the increasing demand for agricultural water, the water availability for winter and spring irrigation of cotton fields has decreased. Consequently, dry seeding followed by irrigation (DSSI) has become a widespread cotton cultivation technique in Xinjiang. This study focused on the interaction [...] Read more.
Due to the increasing demand for agricultural water, the water availability for winter and spring irrigation of cotton fields has decreased. Consequently, dry seeding followed by irrigation (DSSI) has become a widespread cotton cultivation technique in Xinjiang. This study focused on the interaction between soil particles and cotton seeding equipment under DSSI in Xinjiang. The discrete element method (DEM) simulation framework was employed to compare the performance of the Johnson-Kendall-Roberts (JKR) model and Bonding model in simulating contact between soil particles. The models’ ability to simulate the angle of repose was investigated, and shear tests were conducted. The simulation results showed that both models had comparable repose angles, with relative errors of 0.59% for the JKR model and 0.36% for the contact model. However, the contact model demonstrated superior predictive accuracy in simulating direct shear test results, predicting an internal friction angle of 35.8°, with a relative error of 5.8% compared to experimental measurements. In contrast, the JKR model exhibited a larger error. The Bonding model provides a more accurate description of soil particle contact. Subsoiler penetration tests showed that the maximum penetration force was 467.2 N, closely matching the simulation result of 485.3 N, which validates the reliability of the model parameters. The proposed soil simulation framework and calibrated parameters accurately represented soil mechanical properties, providing a robust basis for discrete element modeling and structural optimization of soil-tool interactions in cotton field tillage machinery. Full article
(This article belongs to the Section Agricultural Technology)
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21 pages, 4392 KiB  
Article
Visualization of Kinetic Parameters of a Droplet Nucleation Boiling on Smooth and Micro-Pillar Surfaces with Inclined Angles
by Yi-Nan Zhang, Guo-Qing Huang, Lu-Ming Zhao and Hong-Xia Chen
Energies 2025, 18(15), 4152; https://doi.org/10.3390/en18154152 - 5 Aug 2025
Abstract
The evaporation dynamics of droplets on smooth and inclined micro-pillar surfaces were experimentally investigated. The surface temperature was increased from 50 °C to 120 °C, with the inclination angles being 0°, 30°, 45°, and 60° respectively. The dynamic parameters, including contact area, nucleation [...] Read more.
The evaporation dynamics of droplets on smooth and inclined micro-pillar surfaces were experimentally investigated. The surface temperature was increased from 50 °C to 120 °C, with the inclination angles being 0°, 30°, 45°, and 60° respectively. The dynamic parameters, including contact area, nucleation density, bubble stable diameter, and droplet asymmetry, were recorded using two high-speed video cameras, and the corresponding evaporation performance was analyzed. Experimental results showed that the inclination angle had a significant influence on the evaporation of micro-pillar surfaces than smooth surfaces as well as a positive correlation between the enhancement performance of the micro-pillars and increasing inclination angles. This angular dependence arises from surface inclination-induced tail elongation and the corresponding asymmetry of droplets. With definition of the one-dimensional asymmetry factor (ε) and volume asymmetry factor (γ), it was proven that although the asymmetric thickness of the droplets reduces the nucleation density and bubble stable diameter, the droplet asymmetry significantly increased the heat exchange area, resulting in a 37% improvement in the evaporation rate of micro-pillar surfaces and about a 15% increase in its enhancement performance to smooth surfaces when the inclination angle increased from 0°to 60°. These results indicate that asymmetry causes changes in heat transfer conditions, specifically, a significant increase in the wetted area and deformation of the liquid film, which are the direct enhancement mechanisms of inclined micro-pillar surfaces. Full article
(This article belongs to the Special Issue Advancements in Heat Transfer and Fluid Flow for Energy Applications)
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15 pages, 628 KiB  
Article
Accurate Nonrelativistic Energy Calculations for Helium 1snp1,3P (n = 2 to 27) States via Correlated B-Spline Basis Functions
by Jing Chi, Hao Fang, Yong-Hui Zhang, Xiao-Qiu Qi, Li-Yan Tang and Ting-Yun Shi
Atoms 2025, 13(8), 72; https://doi.org/10.3390/atoms13080072 - 4 Aug 2025
Abstract
Rydberg atoms play a crucial role in testing atomic structure theory, quantum computing and simulation. Measurements of transition frequencies from the 21,3S states to Rydberg P1,3 states have reached a precision of several kHz, which poses [...] Read more.
Rydberg atoms play a crucial role in testing atomic structure theory, quantum computing and simulation. Measurements of transition frequencies from the 21,3S states to Rydberg P1,3 states have reached a precision of several kHz, which poses significant challenges for theoretical calculations, since the accuracy of variational energy calculations decreases rapidly with increasing principal quantum number n. Recently the complex “triple” Hylleraas basis was employed to attain the ionization energy of helium 24P1 state with high accuracy. Different from it, we extended the correlated B-spline basis functions (C-BSBFs) to calculate the Rydberg states of helium. The nonrelativistic energies of 1snpP1,3 states up to n=27 achieve at least 14 significant digits using a unified basis set, thereby greatly reducing the complexity of the optimization process. Results of geometric structure parameters and cusp conditions were presented as well. Both the global operator and direct calculation methods are employed and cross-checked for contact potentials. This C-BSBF method not only obtains high-accuracy energies across all studied levels but also confirms the effectiveness of the C-BSBFs in depicting long-range and short-range correlation effects, laying a solid foundation for future high-accuracy Rydberg-state calculations with relativistic and QED corrections included in helium atom and low-Z helium-like ions. Full article
(This article belongs to the Special Issue Atom and Plasma Spectroscopy)
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25 pages, 2418 KiB  
Review
Contactless Vital Sign Monitoring: A Review Towards Multi-Modal Multi-Task Approaches
by Ahmad Hassanpour and Bian Yang
Sensors 2025, 25(15), 4792; https://doi.org/10.3390/s25154792 - 4 Aug 2025
Abstract
Contactless vital sign monitoring has emerged as a transformative healthcare technology, enabling the assessment of vital signs without physical contact with the human body. This review comprehensively reviews the rapidly evolving landscape of this field, with particular emphasis on multi-modal sensing approaches and [...] Read more.
Contactless vital sign monitoring has emerged as a transformative healthcare technology, enabling the assessment of vital signs without physical contact with the human body. This review comprehensively reviews the rapidly evolving landscape of this field, with particular emphasis on multi-modal sensing approaches and multi-task learning paradigms. We systematically categorize and analyze existing technologies based on sensing modalities (vision-based, radar-based, thermal imaging, and ambient sensing), integration strategies, and application domains. The paper examines how artificial intelligence has revolutionized this domain, transitioning from early single-modality, single-parameter approaches to sophisticated systems that combine complementary sensing technologies and simultaneously extract multiple vital sign parameters. We discuss the theoretical foundations and practical implementations of multi-modal fusion, analyzing signal-level, feature-level, decision-level, and deep learning approaches to sensor integration. Similarly, we explore multi-task learning frameworks that leverage the inherent relationships between vital sign parameters to enhance measurement accuracy and efficiency. The review also critically addresses persisting technical challenges, clinical limitations, and ethical considerations, including environmental robustness, cross-subject variability, sensor fusion complexities, and privacy concerns. Finally, we outline promising future directions, from emerging sensing technologies and advanced fusion architectures to novel application domains and privacy-preserving methodologies. This review provides a holistic perspective on contactless vital sign monitoring, serving as a reference for researchers and practitioners in this rapidly advancing field. Full article
(This article belongs to the Section Biomedical Sensors)
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17 pages, 7323 KiB  
Article
Line Laser 3D Measurement Method and Experiments of Gears
by Yanqiang Sun, Zhaoyao Shi, Bo Yu and Meichuan Li
Photonics 2025, 12(8), 782; https://doi.org/10.3390/photonics12080782 - 4 Aug 2025
Viewed by 83
Abstract
Line laser measurement, as a typical method of laser triangulation, makes the acquisition of 3D tooth-surface data more accurate, efficient, and informative. Thus, a line laser 3D measurement model of gears is established, and a specialized polyhedral artifact with specific geometric features is [...] Read more.
Line laser measurement, as a typical method of laser triangulation, makes the acquisition of 3D tooth-surface data more accurate, efficient, and informative. Thus, a line laser 3D measurement model of gears is established, and a specialized polyhedral artifact with specific geometric features is invented to determine the pose parameters of the line laser sensor in measuring space. Based on this, a single-spindle gear-measuring instrument is developed and a series of experimental studies are conducted for gears with different module and flank directions in this instrument, including profile deviation, helix deviation, pitch deviation, topological deviation, etc. A comparative experiment with traditional contact measurement methods validates the correctness of the methods mentioned in this paper for the accurate evaluation of tested gears. In further research, the mining and utilization of big data obtained from the line laser 3D measurement of gears will be an important topic. Full article
(This article belongs to the Special Issue Advancements in Optical Metrology and Imaging)
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22 pages, 5293 KiB  
Article
Membrane Distillation for Water Desalination: Assessing the Influence of Operating Conditions on the Performance of Serial and Parallel Connection Configurations
by Lebea N. Nthunya and Bhekie B. Mamba
Membranes 2025, 15(8), 235; https://doi.org/10.3390/membranes15080235 - 4 Aug 2025
Viewed by 164
Abstract
Though the pursuit of sustainable desalination processes with high water recovery has intensified the research interest in membrane distillation (MD), the influence of module connection configuration on performance stability remains poorly explored. The current study provided a comprehensive multiparameter assessment of hollow fibre [...] Read more.
Though the pursuit of sustainable desalination processes with high water recovery has intensified the research interest in membrane distillation (MD), the influence of module connection configuration on performance stability remains poorly explored. The current study provided a comprehensive multiparameter assessment of hollow fibre membrane modules connected in parallel and series in direct contact membrane distillation (DCMD) for the first time. The configurations were evaluated under varying process parameters such as temperature (50–70 °C), flow rates (22.1–32.3 mL·s−1), magnesium concentration as scalant (1.0–4.0 g·L−1), and flow direction (co-current and counter-current), assessing their influence on temperature gradients (∆T), flux and pH stability, salt rejection, and crystallisation. Interestingly, the parallel module configuration maintained high operational stability with uniform flux and temperature differences (∆T) even at high recovery factors (>75%). On one hand, the serial configuration experienced fluctuating ∆T caused by thermal and concentration polarisation, causing an early crystallisation (abrupt drop in feed conductivity). Intensified polarisation effects with accelerated crystallisation increased the membrane risk of wetting, particularly at high recovery factors. Despite these changes, the salt rejection remained relatively high (99.9%) for both configurations across all tested conditions. The findings revealed that acidification trends caused by MgSO4 were configuration-dependent, where the parallel setup-controlled rate of pH collapse. This study presented a novel framework connecting membrane module architecture to mass and heat transfer phenomena, providing a transformative DCMD module configuration design in water desalination. These findings not only provide the critical knowledge gaps in DCMD module configurations but also inform optimisation of MD water desalination to achieve high recovery and stable operation conditions under realistic brine composition. Full article
(This article belongs to the Special Issue Membrane Distillation: Module Design and Application Performance)
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25 pages, 7708 KiB  
Review
A Review of Heat Transfer and Numerical Modeling for Scrap Melting in Steelmaking Converters
by Mohammed B. A. Hassan, Florian Charruault, Bapin Rout, Frank N. H. Schrama, Johannes A. M. Kuipers and Yongxiang Yang
Metals 2025, 15(8), 866; https://doi.org/10.3390/met15080866 (registering DOI) - 1 Aug 2025
Viewed by 226
Abstract
Steel is an important product in many engineering sectors; however, steelmaking remains one of the largest CO2 emitters. Therefore, new governmental policies drive the steelmaking industry toward a cleaner and more sustainable operation such as the gas-based direct reduction–electric arc furnace process. [...] Read more.
Steel is an important product in many engineering sectors; however, steelmaking remains one of the largest CO2 emitters. Therefore, new governmental policies drive the steelmaking industry toward a cleaner and more sustainable operation such as the gas-based direct reduction–electric arc furnace process. To become carbon neutral, utilizing more scrap is one of the feasible solutions to achieve this goal. Addressing knowledge gaps regarding scrap heterogeneity (size, shape, and composition) is essential to evaluate the effects of increased scrap ratios in basic oxygen furnace (BOF) operations. This review systematically examines heat and mass transfer correlations relevant to scrap melting in BOF steelmaking, with a focus on low Prandtl number fluids (thick thermal boundary layer) and dense particulate systems. Notably, a majority of these correlations are designed for fluids with high Prandtl numbers. Even for the ones tailored for low Prandtl, they lack the introduction of the porosity effect which alters the melting behavior in such high temperature systems. The review is divided into two parts. First, it surveys heat transfer correlations for single elements (rods, spheres, and prisms) under natural and forced convection, emphasizing their role in predicting melting rates and estimating maximum shell size. Second, it introduces three numerical modeling approaches, highlighting that the computational fluid dynamics–discrete element method (CFD–DEM) offers flexibility in modeling diverse scrap geometries and contact interactions while being computationally less demanding than particle-resolved direct numerical simulation (PR-DNS). Nevertheless, the review identifies a critical gap: no current CFD–DEM framework simultaneously captures shell formation (particle growth) and non-isotropic scrap melting (particle shrinkage), underscoring the need for improved multiphase models to enhance BOF operation. Full article
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16 pages, 4508 KiB  
Article
Natural Kelp (Laminaria japonica) Hydrogel with Anisotropic Mechanical Properties, Low Friction and Self-Cleaning for Triboelectric Nanogenerator
by Dongnian Chen, Hui Yu, Jiajia Hao, Qiang Chen and Lin Zhu
Gels 2025, 11(8), 597; https://doi.org/10.3390/gels11080597 - 1 Aug 2025
Viewed by 122
Abstract
Kelp is a natural hydrogel material, which has been widely used in food industry. However, as a natural material, its properties have not been well explored. In this work, the surface and mechanical properties of kelp were investigated. The surface of kelp exhibited [...] Read more.
Kelp is a natural hydrogel material, which has been widely used in food industry. However, as a natural material, its properties have not been well explored. In this work, the surface and mechanical properties of kelp were investigated. The surface of kelp exhibited superoleophobicity and a self-clean property. The friction coefficient (COF) of the kelp surface was also low (<0.1). Interestingly, kelp demonstrated anisotropic mechanical properties either with or without metal ions. The tensile strength and toughness of kelp along with the growth direction (H) were better than those at the direction vertical to the growth direction (V). The adsorption of metal ions would significantly enhance the mechanical properties and ionic conductivity. Triboelectric nanogenerator (TENG) was assembled using kelp with NaCl, which showed excellent output performance (open-circuit voltage of 30 V, short-circuit current of 0.73 μA and charge transfer on contact of 10.5 nC). A writing tablet was prepared to use as the kelp-based self-powered tactile sensor. This work provides a new insight into natural kelp, which may be used as a renewable material. Full article
(This article belongs to the Special Issue Applications of Gels in Energy Materials and Devices)
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21 pages, 3864 KiB  
Article
Sub-MHz EMAR for Non-Contact Thickness Measurement: How Ultrasonic Wave Directivity Affects Accuracy
by Alexander Siegl, David Auer, Bernhard Schweighofer, Andre Hochfellner, Gerald Klösch and Hannes Wegleiter
Sensors 2025, 25(15), 4746; https://doi.org/10.3390/s25154746 - 1 Aug 2025
Viewed by 242
Abstract
Electromagnetic acoustic resonance (EMAR) is a well-established non-contact method for ultrasonic thickness measurement, typically operated at frequencies above 1 MHz using an electromagnetic acoustic transducer (EMAT). This study successfully extends EMAR into the sub-MHz range, allowing supply voltages below 60 V and thus [...] Read more.
Electromagnetic acoustic resonance (EMAR) is a well-established non-contact method for ultrasonic thickness measurement, typically operated at frequencies above 1 MHz using an electromagnetic acoustic transducer (EMAT). This study successfully extends EMAR into the sub-MHz range, allowing supply voltages below 60 V and thus offering safer and more cost-effective operation. Experiments were conducted on copper blocks approximately 20 mm thick, where a relative thickness accuracy of better than 0.2% is obtained. Regarding this result, the research identifies a critical design principle: Stable thickness resonances and subsequently accurate thickness measurement are achieved when the ratio of ultrasonic wavelength to EMAT track width (λ/w) falls below 1. This minimizes the excitation and interactions with structural eigenmodes, ensuring consistent measurement reliability. To support this, the study introduces a system-based model to simulate the EMAR method. The model provides detailed insights into how wave propagation affects the accuracy of EMAR measurements. Experimental results align well with the simulation outcome and confirm the feasibility of EMAR in the sub-MHz regime without compromising precision. These findings highlight the potential of low-voltage EMAR as a safer, cost-effective, and highly accurate approach for industrial ultrasonic thickness measurements. Full article
(This article belongs to the Special Issue Electromagnetic Sensing and Its Applications)
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24 pages, 4217 KiB  
Article
Contact Load Measurement and Validation for Tapered Rollers in Wind Turbine Main Bearing
by Zhenggang Guo, Jingqi Yu, Wanxiu Hao and Yuming Niu
Sensors 2025, 25(15), 4726; https://doi.org/10.3390/s25154726 - 31 Jul 2025
Viewed by 229
Abstract
Addressing the need for contact load detection in wind turbine main bearings during service, a roller contact load measurement method is proposed. An analytical model characterizes the contact load-to-inner bore strain mapping relationship. To overcome the inherent low sensitivity of direct bore strain [...] Read more.
Addressing the need for contact load detection in wind turbine main bearings during service, a roller contact load measurement method is proposed. An analytical model characterizes the contact load-to-inner bore strain mapping relationship. To overcome the inherent low sensitivity of direct bore strain measurement, bore-to-measurement-point sensitivity analysis was optimized. Multiple structurally optimized sensor brackets were designed to enhance strain measurement sensitivity, and their performance was comparatively evaluated via simulation. To mitigate sensitivity fluctuations caused by roller rotation phase variations, a strain–phase–load calculation method incorporating real-time phase compensation was developed and verified through simulation analysis. A dedicated roller contact load testing system was constructed and experimental validation was conducted. Results demonstrate 95% accuracy in contact load acquisition. This method accurately obtains roller contact loads in wind turbine main bearings, proving crucial for studying bearing mechanical behavior, predicting fatigue life, optimizing structural design, and enhancing reliability. Full article
(This article belongs to the Section Fault Diagnosis & Sensors)
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33 pages, 4142 KiB  
Review
Advances in Wettability-Engineered Open Planar-Surface Droplet Manipulation
by Ge Chen, Jin Yan, Junjie Liang, Jiajia Zheng, Jinpeng Wang, Hongchen Pang, Xianzhang Wang, Zihao Weng and Wei Wang
Micromachines 2025, 16(8), 893; https://doi.org/10.3390/mi16080893 (registering DOI) - 31 Jul 2025
Viewed by 310
Abstract
Firstly, this paper reviews the fundamental theories of solid surface wettability and contact angle hysteresis. Subsequently, it further introduces four typical wettability-engineered surfaces with low hysteresis (superhydrophobic, superamphiphobic, super-slippery, and liquid-like smooth surfaces). Finally, it focuses on the latest research progress in the [...] Read more.
Firstly, this paper reviews the fundamental theories of solid surface wettability and contact angle hysteresis. Subsequently, it further introduces four typical wettability-engineered surfaces with low hysteresis (superhydrophobic, superamphiphobic, super-slippery, and liquid-like smooth surfaces). Finally, it focuses on the latest research progress in the field of droplet manipulation on open planar surfaces with engineered wettability. To achieve droplet manipulation, the core driving forces primarily stem from natural forces guided by bioinspired gradient surfaces or the regulatory effects of external fields. In terms of bioinspired self-propelled droplet movement, this paper summarizes research inspired by natural organisms such as desert beetles, cacti, self-aligning floating seeds of emergent plants, or water-walking insects, which construct bioinspired special gradient surfaces to induce Laplace pressure differences or wettability gradients on both sides of droplets for droplet manipulation. Moreover, this paper further analyzes the mechanisms, advantages, and limitations of these self-propelled approaches, while summarizing the corresponding driving force sources and their theoretical formulas. For droplet manipulation under external fields, this paper elaborates on various external stimuli including electric fields, thermal fields, optical fields, acoustic fields, and magnetic fields. Among them, electric fields involve actuation mechanisms such as directly applied electrostatic forces and indirectly applied electrocapillary forces; thermal fields influence droplet motion through thermoresponsive wettability gradients and thermocapillary effects; optical fields cover multiple wavelengths including near-infrared, ultraviolet, and visible light; acoustic fields utilize horizontal and vertical acoustic radiation pressure or acoustic wave-induced acoustic streaming for droplet manipulation; the magnetic force acting on droplets may originate from their interior, surface, or external substrates. Based on these different transport principles, this paper comparatively analyzes the unique characteristics of droplet manipulation under the five external fields. Finally, this paper summarizes the current challenges and issues in the research of droplet manipulation on the open planar surfaces and provides an outlook on future development directions in this field. Full article
(This article belongs to the Special Issue Advanced Microfluidic Chips: Optical Sensing and Detection)
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19 pages, 3130 KiB  
Article
Deep Learning-Based Instance Segmentation of Galloping High-Speed Railway Overhead Contact System Conductors in Video Images
by Xiaotong Yao, Huayu Yuan, Shanpeng Zhao, Wei Tian, Dongzhao Han, Xiaoping Li, Feng Wang and Sihua Wang
Sensors 2025, 25(15), 4714; https://doi.org/10.3390/s25154714 - 30 Jul 2025
Viewed by 224
Abstract
The conductors of high-speed railway OCSs (Overhead Contact Systems) are susceptible to conductor galloping due to the impact of natural elements such as strong winds, rain, and snow, resulting in conductor fatigue damage and significantly compromising train operational safety. Consequently, monitoring the galloping [...] Read more.
The conductors of high-speed railway OCSs (Overhead Contact Systems) are susceptible to conductor galloping due to the impact of natural elements such as strong winds, rain, and snow, resulting in conductor fatigue damage and significantly compromising train operational safety. Consequently, monitoring the galloping status of conductors is crucial, and instance segmentation techniques, by delineating the pixel-level contours of each conductor, can significantly aid in the identification and study of galloping phenomena. This work expands upon the YOLO11-seg model and introduces an instance segmentation approach for galloping video and image sensor data of OCS conductors. The algorithm, designed for the stripe-like distribution of OCS conductors in the data, employs four-direction Sobel filters to extract edge features in horizontal, vertical, and diagonal orientations. These features are subsequently integrated with the original convolutional branch to form the FDSE (Four Direction Sobel Enhancement) module. It integrates the ECA (Efficient Channel Attention) mechanism for the adaptive augmentation of conductor characteristics and utilizes the FL (Focal Loss) function to mitigate the class-imbalance issue between positive and negative samples, hence enhancing the model’s sensitivity to conductors. Consequently, segmentation outcomes from neighboring frames are utilized, and mask-difference analysis is performed to autonomously detect conductor galloping locations, emphasizing their contours for the clear depiction of galloping characteristics. Experimental results demonstrate that the enhanced YOLO11-seg model achieves 85.38% precision, 77.30% recall, 84.25% AP@0.5, 81.14% F1-score, and a real-time processing speed of 44.78 FPS. When combined with the galloping visualization module, it can issue real-time alerts of conductor galloping anomalies, providing robust technical support for railway OCS safety monitoring. Full article
(This article belongs to the Section Industrial Sensors)
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13 pages, 1186 KiB  
Article
Targeting the Cell Wall Salvage Pathway: Dual-Enzyme Inhibition of AmgK and MurU as a Strategy Against Antibiotic Resistance
by Hwa Young Kim, Seri Jo, Mi-Sun Kim and Dong Hae Shin
Int. J. Mol. Sci. 2025, 26(15), 7368; https://doi.org/10.3390/ijms26157368 - 30 Jul 2025
Viewed by 210
Abstract
The rise of multidrug-resistant Pseudomonas aeruginosa underscores the need for novel therapeutic targets beyond conventional peptidoglycan biosynthesis. Some bacterial strains bypass MurA inhibition by fosfomycin via a cell wall salvage pathway. This study targeted P. aeruginosa AmgK (PaAmgK) and MurU ( [...] Read more.
The rise of multidrug-resistant Pseudomonas aeruginosa underscores the need for novel therapeutic targets beyond conventional peptidoglycan biosynthesis. Some bacterial strains bypass MurA inhibition by fosfomycin via a cell wall salvage pathway. This study targeted P. aeruginosa AmgK (PaAmgK) and MurU (PaMurU) to identify inhibitors that could complement fosfomycin therapy. A malachite-green-based dual-enzyme assay enabled efficient activity measurements and high-throughput chemical screening. Screening 232 compounds identified Congo red and CTAB as potent PaMurU inhibitors. A targeted mass spectrometric analysis confirmed the selective inhibition of PaMurU relative to that of PaAmgK. Molecular docking simulations indicate that Congo red preferentially interacts with PaMurU through electrostatic contacts, primarily involving the residues Arg28 and Arg202. The binding of Congo red to PaMurU was corroborated further using SUPR-differential scanning fluorimetry (SUPR-DSF), which revealed ligand-induced thermal destabilization. Ongoing X-ray crystallographic studies, in conjunction with site-directed mutagenesis and enzyme kinetic analyses, aim to elucidate the binding mode at an atomic resolution. Full article
(This article belongs to the Section Molecular Microbiology)
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22 pages, 3894 KiB  
Article
3D-Printed Biocompatible Frames for Electrospun Nanofiber Membranes: An Enabling Biofabrication Technology for Three-Dimensional Tissue Models and Engineered Cell Culture Platforms
by Adam J. Jones, Lauren A. Carothers, Finley Paez, Yanhao Dong, Ronald A. Zeszut and Russell Kirk Pirlo
Micromachines 2025, 16(8), 887; https://doi.org/10.3390/mi16080887 - 30 Jul 2025
Viewed by 433
Abstract
Electrospun nanofiber membranes (ESNFMs) are exceptional biomaterials for tissue engineering, closely mimicking the native extracellular matrix. However, their inherent fragility poses significant handling, processing, and integration challenges, limiting their widespread application in advanced 3D tissue models and biofabricated devices. This study introduces a [...] Read more.
Electrospun nanofiber membranes (ESNFMs) are exceptional biomaterials for tissue engineering, closely mimicking the native extracellular matrix. However, their inherent fragility poses significant handling, processing, and integration challenges, limiting their widespread application in advanced 3D tissue models and biofabricated devices. This study introduces a novel and on-mat framing technique utilizing extrusion-based printing of a UV-curable biocompatible resin (Biotough D90 MF) to create rigid, integrated support structures directly on chitosan–polyethylene oxide (PEO) ESNFMs. We demonstrate fabrication of these circular frames via precise 3D printing and a simpler manual stamping method, achieving robust mechanical stabilization that enables routine laboratory manipulation without membrane damage. The resulting framed ESNFMs maintain structural integrity during subsequent processing and exhibit excellent biocompatibility in standardized extract assays (116.5 ± 12.2% normalized cellular response with optimized processing) and acceptable performance in direct contact evaluations (up to 78.2 ± 32.4% viability in the optimal configuration). Temporal assessment revealed characteristic cellular adaptation dynamics on nanofiber substrates, emphasizing the importance of extended evaluation periods for accurate biocompatibility determination of three-dimensional scaffolds. This innovative biofabrication approach overcomes critical limitations of previous handling methods, transforming delicate ESNFMs into robust, easy-to-use components for reliable integration into complex cell culture applications, barrier tissue models, and engineered systems. Full article
(This article belongs to the Special Issue Advanced Biomaterials and Biofabrication)
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