Search Results (23)

Olive knot disease, caused by Pseudomonas savastanoi pv. savastanoi, severely impacts olive tree yield and oil quality. Early and accurate detection of the bacterium’s presence, particularly in asymptomatic plants, is crucial for effective disease management. This study aimed to develop an improved protocol for processing plant samples and adapting quantitative PCR to droplet digital PCR (ddPCR). For this purpose, four plant preparations—EW (external washing), PELLET (bacterial concentration), and enrichment in liquid media for 24 or 48 h (24hE, 48hE)—were tested using spiked samples. The ddPCR was set up and compared with qPCR to evaluate analytical sensitivity and specificity. Additionally, field samples from symptomatic and asymptomatic olive orchards were tested to evaluate the performance of the selected methods in naturally infected plants. ddPCR showed higher sensitivity than qPCR, particularly with the PELLET and 24hE preparations. The PELLET from the spiked sample preparation achieved a limit of detection of 10 CFU/mL for both molecular tests. The ddPCR, combined with the PELLET preparation, offers a highly sensitive and reliable tool for detecting P. savastanoi pv. savastanoi in asymptomatic olive material. This protocol shows great potential for improving early bacterial detection and disease prevention, thus aiding control strategies in nurseries and olive orchards, and supporting the production of certified plant propagation material. Full article

The present paper aims to quantify the carbon emissions associated with the establishment of 15 walnut orchards (“Juglans californica”) in the greater area of Magnisia, Greece, with the use of a carbon footprint tool interconnected to a Farm Management Information System. The data collection spanned the first five years following the planting of the trees, providing a comprehensive view of the emissions during this critical establishment phase. Over the five-year period examined (February 2019–December 2023), the results revealed net carbon emissions amounting to 13.71 tn CO₂ eq ha⁻¹, with the calculated emissions showing an increasing trend from the first year through the fifth year. Scope 1 (7.38 tn CO₂ eq ha⁻¹) and Scope 2 (3.71 tn CO₂ eq ha⁻¹) emissions emerged as the most significant, while irrigation (drip irrigation) and fertilizing practices were identified as the highest contributors to emissions. This study highlights the significance of using integrated digital tools for monitoring the performance of cultivations rather than standalone tools that are currently widely available. Integrated tools that incorporate various applications simplify data collection, encourage accurate record-keeping, and facilitate certification processes. By automating data entry and calculations, these tools reduce human error during agricultural carbon management and save time; thus, the integration of digital monitoring tools is vital in improving data accuracy, streamlining certification processes, and promoting eco-friendly practices, crucial for the evolving carbon market. Full article

The Leaf Area Index (LAI) strongly influences vegetation evapotranspiration and photosynthesis rates. Timely and accurately estimating the LAI is crucial for monitoring vegetation growth. The unmanned aerial vehicle (UAV) multispectral digital camera platform has been proven to be an effective tool for this purpose. Currently, most remote sensing estimations of LAIs focus on cereal crops, with limited research on economic crops such as apples. In this study, a method for estimating the LAI of an apple orchard by extracting spectral and texture information from UAV multispectral images was proposed. Specifically, field measurements were conducted to collect LAI data for 108 sample points during the final flowering (FF), fruit setting (FS), and fruit expansion (FE) stages of apple growth in 2023. Concurrently, UAV multispectral images were obtained to extract spectral and texture information (Gabor transform). The Support Vector Regression Recursive Feature Elimination (SVR-REF) was employed to select optimal features as inputs for constructing models to estimate the LAI. Finally, the optimal model was used for LAI mapping. The results indicate that integrating spectral and texture information effectively enhances the accuracy of LAI estimation, with the relative prediction deviation (RPD) for all models being greater than 2. The Categorical Boosting (CatBoost) model established for FF exhibits the highest accuracy, with a validation set R², root mean square error (RMSE), and RPD of 0.867, 0.203, and 2.482, respectively. UAV multispectral imagery proves to be valuable in estimating apple orchard LAIs, offering real-time monitoring of apple growth and providing a scientific basis for orchard management. Full article

Precision agriculture-based orchard management relies heavily on the accurate delineation of tree canopies, especially for high-value crops like mangoes. Traditional GIS and remote sensing methods, such as Object-Based Imagery Analysis (OBIA), often face challenges due to overlapping canopies, complex tree structures, and varied light conditions. This study aims to enhance the accuracy of mango orchard mapping by developing a novel deep-learning approach that combines fine-tuned object detection and segmentation techniques. UAV imagery was collected over a 65-acre mango orchard in Multan, Pakistan, and processed into an RGB orthomosaic with a 3 cm ground sampling distance. The You Only Look Once (YOLOv7) framework was trained on an annotated dataset to detect individual mango trees. The resultant bounding boxes were used as prompts for the segment anything model (SAM) for precise delineation of canopy boundaries. Validation against ground truth data of 175 manually digitized trees showed a strong correlation ($R^2$ = 0.97), indicating high accuracy and minimal bias. The proposed method achieved a mean absolute percentage error (MAPE) of 4.94% and root mean square error (RMSE) of 80.23 sq ft against manually digitized tree canopies with an average size of 1290.14 sq ft. The proposed approach effectively addresses common issues such as inaccurate bounding boxes and over- or under-segmentation of tree canopies. The enhanced accuracy can substantially assist in various downstream tasks such as tree location mapping, canopy volume estimation, health monitoring, and crop yield estimation. Full article

The educational problems in the area, economic disparities, conflict situations, and deficiencies in educational infrastructure directly affect the quality and accessibility of education. Therefore, the present research aims to generate comfort for users of the educational center by applying sustainable design strategies in Carabayllo, Peru. The study started with a literature review, an analysis of flora and fauna, passive design strategies, and climatic analysis applying sustainability strategies supported by digital tools (AutoCAD, Revit Collaborate, Climate Consultant, OpenStreetMap, JOSM, Rhinoceros, and Grasshopper). As a result, the design proposes an educational center that ensures year-round comfort through energy efficiency, the use of eco-friendly materials, and green roofs. Additionally, it includes the implementation of dry toilets, biofilters, and xerophytic vegetation for orchards, promoting food production and enhancing the treatment of nearby public spaces. In conclusion, this proposal enhances the quality of life for users by applying passive design strategies and sustainability principles, adopting clean energy sources, and efficiently managing waste, thereby contributing to the Sustainable Development Goals (SDGs). Full article

The uses of precision oliviculture have increased in recent years to improve the quality and quantity of extra virgin olive oil. In traditional and intensive systems, biennial pruning is often applied to balance and maintain plant vigour, aiming at reducing management costs. This study presents the results of a three-year experiment with the objective of quantifying the effects of biennial pruning on the vegetative vigour of olive trees, investigating the geometric and spectral characteristics of each canopy determined with multispectral images acquired by UAV. The experiment was carried out in an olive orchard located in western Sicily (Italy). Multispectral images were acquired using a UAV in automatic flight configuration at an altitude of 70 m a.g.l. The segmentation and classification of the images were performed using Object-Based Image Analysis (OBIA) based on the Digital Elevation Model (DEM) and orthomosaic to extract the canopy area, height, volume and NDVI for each plant. This study showed that the technology and image analysis processing used were able to estimate vigour parameters at different canopy densities, compared to field measurements (R² = 0.97 and 0.96 for canopy area and volume, respectively). Furthermore, it was possible to determine the amount of removed biomass for each plant and vigour level. Biennial pruning decreased the number of plants initially classified as LV (low-vigour) and maintained a vegetative balance for MV (medium-vigour) and HV (high-vigour) plants, reducing the spatial variability in the field. Full article

In the context of Industry 4.0, one of the most significant challenges is enhancing efficiency in sectors like agriculture by using intelligent sensors and advanced computing. Specifically, the task of fruit detection and counting in orchards represents a complex issue that is crucial for efficient orchard management and harvest preparation. Traditional techniques often fail to provide the timely and precise data necessary for these tasks. With the agricultural sector increasingly relying on technological advancements, the integration of innovative solutions is essential. This study presents a novel approach that combines artificial intelligence (AI), deep learning (DL), and unmanned aerial vehicles (UAVs). The proposed approach demonstrates superior real-time capabilities in fruit detection and counting, utilizing a combination of AI techniques and multi-UAV systems. The core innovation of this approach is its ability to simultaneously capture and synchronize video frames from multiple UAV cameras, converting them into a cohesive data structure and, ultimately, a continuous image. This integration is further enhanced by image quality optimization techniques, ensuring the high-resolution and accurate detection of targeted objects during UAV operations. Its effectiveness is proven by experiments, achieving a high mean average precision rate of 86.8% in fruit detection and counting, which surpasses existing technologies. Additionally, it maintains low average error rates, with a false positive rate at 14.7% and a false negative rate at 18.3%, even under challenging weather conditions like cloudiness. Overall, the practical implications of this multi-UAV imaging and DL-based approach are vast, particularly for real-time fruit recognition in orchards, marking a significant stride forward in the realm of digital agriculture that aligns with the objectives of Industry 4.0. Full article

Advances in measurement systems and technologies are being avidly taken up in perennial tree crop research and industry applications. However, there is a lack of a standard model to support streamlined management and integration of the data generated from advanced measurement systems used in tree crop research. Furthermore, the rapid expansion in the diversity and volumes of data is increasingly highlighting the requirement for a comprehensive data model and an ecosystem for efficient orchard management and decision-making. This research focuses on the design and implementation of a novel proof-of-concept data ecosystem that enables improved data storage, management, integration, processing, analysis, and usage. Contemporary technologies proliferating in other sectors but that have had limited adoption in agricultural research have been incorporated into the model. The core of the proposed solution is a service-oriented API-driven system coupled with a standard-based digital orchard model. Applying this solution in Agriculture Victoria’s Tatura tree crop research farm (the Tatura SmartFarm) has significantly reduced overheads in research data management, enhanced analysis, and improved data resolution. This is demonstrated by the preliminary results presented for in-orchard and postharvest data collection applications. The data ecosystem developed as part of this research also establishes a foundation for early fruit traceability across industry and research. Full article

Soil fertility plays a crucial role in crop growth, so it is important to study the spatial distribution and variation of soil fertility for agricultural management and decision-making. However, traditional methods for assessing soil fertility are time-consuming and economically burdensome. Moreover, it is hard to capture the spatial variation of soil properties across continuous geographic space using the conventional methods. As key techniques of digital soil mapping (DSM), spatial interpolation techniques have been widely applied in soil surveys and analysis in recent years, since they can predict soil properties at unknown points in continuous space based on limited sample points. However, further research is needed on spatial interpolation models for DSM in regions with variable climates and complex terrains, which are characterized by strong spatial variation in both environmental variables and soil fertility. In this study, taking a typical hilly area in a subtropical monsoon climate, i.e., Gaozhou, Guangdong Province, China, as an example, the performances of four popular spatial interpolation models (Random Forest (RF), Ordinary Kriging, Inverse Distance Weighting, and Radial Basis Function) for digital soil mapping on available phosphorus (AP) are compared. Based on RF, the spatial variation and its driving factors of the AP of Gaozhou are then analyzed. Furthermore, by selecting three typical truncation lines from different directions, the correlations between environmental variables and AP in different spatial positions are demonstrated. The root mean square error (RMSE) results of the above four models are 32.01, 32.08, 32.74, and 33.08, respectively, which indicate that the RF has a higher interpolation accuracy. Based on the mapping results of RF, the minimum, maximum, and mean values of AP in the study area are 38.90, 95.24, and 64.96 mg/kg, respectively. The high-value areas of AP are mainly distributed in forested and orchard areas, while the low-value areas are primarily found in urban and cultivated areas in the eastern and western regions. Vegetation and topography are identified as the key factors shaping the spatial variations of AP in the study area. Furthermore, the spatial heterogeneity of the influence strength of altitude and EVI is revealed, providing a new direction for further research on DSM in the future, i.e., spatial interpolation models considering the spatial heterogeneity of the influence of environmental variables. Full article

Nitrogen is one of the most important nutrients affecting the growth and fruit quality of walnut trees. Rapid and accurate monitoring of nitrogen concentration in the walnut canopy can provide a digital basis for its nutritional diagnosis and precision fertilization. Consequently, the main purpose of this study was to use Unmanned Aerial Vehicle (UAV) remote sensing technology to monitor the nitrogen concentration of walnut canopies. In this study, UAV multispectral images of the canopies of nine walnut orchards with different management levels in Wensu County, South Xinjiang, China, were collected during the fast-growing (20 May), sclerotization (25 June), and near-maturity (27 August) periods of walnut fruit, and canopy nitrogen concentration data for 180 individual plants were collected during the same periods. The validity of the information extracted via the outline canopy and simulated canopy methods was compared. The accuracy of nitrogen concentration inversion for three modeling methods, partial least squares regression (PLSR), support vector machine (SVM), and random forest (RF), was analyzed; the effects of different combinations of variables on model accuracy were compared; and the spatial distribution of the nitrogen concentration in the walnut canopy was numerically mapped using the optimal model. The results showed that the accuracy of the model created using the single plant information extracted from the outlined canopy was better than that of the simulated canopy method, but the simulated canopy method was more efficient in extracting effective information from the single plant canopy than the outlined canopy. The simulated canopy method overcame the difficulty of mismatching the spectral information of individual plants extracted, by outlining the canopy in the original image for nitrogen distribution mapping with the spectral information of image elements in the original resolution image. The prediction accuracy of the RF model was better than that of the SVM and PLSR models; the prediction accuracy of the model using a combination of waveband texture information and vegetation index texture information was better than that of the single-source model. The coefficients of determination (R²) values of the RF prediction model built using the band texture information extracted via the simulated canopy method with the vegetation index texture information were in the range of 0.61–0.84, the root mean square error (RMSE) values were in the range of 0.27–0.43 g kg⁻¹, and the relative analysis error (RPD) values were in the range of 1.58–2.20. This study shows that it is feasible to monitor the nitrogen concentration of walnut tree canopies using UAV multispectral remote sensing. This study provides a theoretical basis and methodological reference for the rapid monitoring of nutrients in fruit trees in southern Xinjiang. Full article

Precision application of pesticides based on tree canopy characteristics such as tree height is more environmentally friendly and healthier for humans. Offline prescription maps can be used to achieve precise pesticide application at low cost. To obtain a complete point cloud with detailed tree canopy information in orchards, a LiDAR-RTK fusion information acquisition system was developed on an all-terrain vehicle (ATV) with an autonomous driving system. The point cloud was transformed into a geographic coordinate system for registration, and the Random sample consensus (RANSAC) was used to segment it into ground and canopy. A 3D voxel prescription map with a unit size of 0.25 m was constructed from the tree canopy point cloud. The height of 20 trees was geometrically measured to evaluate the accuracy of the voxel prescription map. The results showed that the RMSE between tree height calculated from the LiDAR obtained point cloud and the actual measured tree height was 0.42 m, the relative RMSE (rRMSE) was 10.86%, and the mean of absolute percentage error (MAPE) was 8.16%. The developed LiDAR-RTK fusion acquisition system can generate 3D prescription maps that meet the requirements of precision pesticide application. The information acquisition system of developed LiDAR-RTK fusion could construct 3D prescription maps autonomously that match the application requirements in digital orchard management. Full article

Monitoring fruit tree flowering information in the open world is more crucial than in the research-oriented environment for managing agricultural production to increase yield and quality. This work presents a transformer-based flowering period monitoring approach in an open world in order to better monitor the whole blooming time of modern standardized orchards utilizing IoT technologies. This study takes images of flowering apple trees captured at a distance in the open world as the research object, extends the dataset by introducing the Slicing Aided Hyper Inference (SAHI) algorithm, and establishes an S-YOLO apple flower detection model by substituting the YOLOX backbone network with Swin Transformer-tiny. The experimental results show that S-YOLO outperformed YOLOX-s in the detection accuracy of the four blooming states by 7.94%, 8.05%, 3.49%, and 6.96%. It also outperformed YOLOX-s by 10.00%, 9.10%, 13.10%, and 7.20% for mAP_ALL, mAP_S, mAP_M, and mAP_L, respectively. By increasing the width and depth of the network model, the accuracy of the larger S-YOLO was 88.18%, 88.95%, 89.50%, and 91.95% for each flowering state and 39.00%, 32.10%, 50.60%, and 64.30% for each type of mAP, respectively. The results show that the transformer-based method of monitoring the apple flower growth stage utilized S-YOLO to achieve the apple flower count, percentage analysis, peak flowering time determination, and flowering intensity quantification. The method can be applied to remotely monitor flowering information and estimate flowering intensity in modern standard orchards based on IoT technology, which is important for developing fruit digital production management technology and equipment and guiding orchard production management. Full article

Calculating the complex 3D traits of trees such as branch structure using drones/unmanned aerial vehicles (UAVs) with onboard RGB cameras is challenging because extracting branch skeletons from such image-generated sparse point clouds remains difficult. This paper proposes a skeleton extraction algorithm for the sparse point cloud generated by UAV RGB images with photogrammetry. We conducted a comparison experiment by flying a UAV from two altitudes (50 m and 20 m) above a university orchard with several fruit tree species and developed three metrics, namely the F1-score of bifurcation point (FBP), the F1-score of end point (FEP), and the Hausdorff distance (HD) to evaluate the performance of the proposed algorithm. The results show that the average values of FBP, FEP, and HD for the point cloud of fruit tree branches collected at 50 m altitude were 64.15%, 69.94%, and 0.0699, respectively, and those at 20 m were 83.24%, 84.66%, and 0.0474, respectively. This paper provides a branch skeleton extraction method for low-cost 3D digital management of orchards, which can effectively extract the main skeleton from the sparse fruit tree branch point cloud, can assist in analyzing the growth state of different types of fruit trees, and has certain practical application value in the management of orchards. Full article

The detection and counting of fruit tree canopies are important for orchard management, yield estimation, and phenotypic analysis. Previous research has shown that most fruit tree canopy detection methods are based on the use of traditional computer vision algorithms or machine learning methods to extract shallow features such as color and contour, with good results. However, due to the lack of robustness of these features, most methods are hardly adequate for the recognition and counting of fruit tree canopies in natural scenes. Other studies have shown that deep learning methods can be used to perform canopy detection. However, the adhesion and occlusion of fruit tree canopies, as well as background noise, limit the accuracy of detection. Therefore, to improve the accuracy of fruit tree canopy recognition and counting in real-world scenarios, an improved YOLOv4 (you only look once v4) is proposed, using a dataset produced from fruit tree canopy UAV imagery, combined with the Mobilenetv3 network, which can lighten the model and increase the detection speed, combined with the CBAM (convolutional block attention module), which can increase the feature extraction capability of the network, and combined with ASFF (adaptively spatial feature fusion), which enhances the multi-scale feature fusion capability of the network. In addition, the K-means algorithm and linear scale scaling are used to optimize the generation of pre-selected boxes, and the learning strategy of cosine annealing is combined to train the model, thus accelerating the training speed of the model and improving the detection accuracy. The results show that the improved YOLOv4 model can effectively overcome the noise in an orchard environment and achieve fast and accurate recognition and counting of fruit tree crowns while lightweight the model. The mAP reached 98.21%, FPS reached 96.25 and F1-score reached 93.60% for canopy detection, with a significant reduction in model size; the average overall accuracy (AOA) reached 96.73% for counting. In conclusion, the YOLOv4-Mobilenetv3-CBAM-ASFF-P model meets the practical requirements of orchard fruit tree canopy detection and counting in this study, providing optional technical support for the digitalization, refinement, and smart development of smart orchards. Full article

A digital elevation model (DEM) represents the topographic surface of the Earth and is an indispensable source of data in many applications, such as flood modeling, infrastructure design and land management. DEM data at high spatial resolution and high accuracy of elevation data are not only costly and time-consuming to acquire but also often confidential. In this paper, we explore a cost-effective approach to derive good quality DEM data by applying a multi-channel convolutional neural network (CNN) to enhance free resources of available DEM data. Shuttle Radar Topography Mission (SRTM) data, multi-spectral imaging Sentinel-2, as well as Google satellite imagery were used as inputs to the CNN model. The CNN model was first trained using high-quality reference DEM data in a dense urban city—Nice, France—then validated on another site in Nice and finally tested in the Orchard Road area (Singapore), which is also an equally dense urban area in Singapore. The CNN model not only shows an impressive reduction in the root mean square error (RMSE) of 50% at validation site in Nice and 30% at the test site in Singapore, but also results in much clearer profiles of the land surface than input SRTM data. A comparison between CNN performance and that of an earlier conducted study using artificial neural networks (ANN) was conducted as well. The comparison within this limited study shows that CNN yields a more accurate DEM. Full article