Search Results (152)

Climate change is reshaping veterinary parasite transmission by altering thermal and hydrological suitability, environmental stage persistence, vector and intermediate host ecology, and contact across livestock–wildlife–companion animal interfaces. These effects are nonlinear; while warming may extend transmission in some systems, heat, desiccation, habitat loss, or disrupted hydrology can reduce the risk or concentrate transmission in local refugia. This critical narrative review compares pasture-transmitted helminths, snail-borne trematodes, environmentally transmitted protozoa, vector-borne parasites, and multi-host cycles. We propose an attribution framework that classifies observed changes across four dimensions (geographic range, seasonal timing, transmission intensity, and host-interface structure) and evaluates them through five analytical filters: suitability, parasite life-cycle response, vector or intermediate-host response, host-interface change, and surveillance artifacts. This framework prevents improved detection, land-use change, animal movement, management shifts and improved detection from being mistaken for climate-driven emergence. We also propose a climate–refugia paradox hypothesis, requiring empirical validation, in which drought or heat may reduce unselected parasite refugia and intensify selection for anthelmintic resistance. Finally, we connect a tiered diagnostic approach from field tools to reference molecular surveillance to support attribution-aware, risk-based One Health strategies that protect animal production, biodiversity, and public health. Full article

Salmonella enterica subspecies enterica serovar Senftenberg comprises a heterogeneous group of nontyphoidal Salmonella strains frequently detected in animal feed, feed ingredients, feed-manufacturing facilities, poultry production, and a wide range of foods. Genomic analyses indicate that S. Senftenberg is polyphyletic, comprising multiple genetically distinct evolutionary lineages and considerable phenotypic variation among isolates. Certain strains exhibit remarkable tolerance to heat, desiccation, and routine cleaning and disinfection procedures, facilitating their persistence in these environments and potentially contributing to transmission through the food chain. Human infections associated with S. Senftenberg range from self-limiting gastroenteritis to invasive disease, while some outbreak-associated isolates have demonstrated resistance to antimicrobials of particular importance in human medicine, adaptation to plant-derived antimicrobial compounds, and unusual virulence profiles. This review examines S. Senftenberg as an example of how adaptive traits can contribute to the persistence of Salmonella under environmental and selective pressures, with emphasis on its occurrence and persistence in poultry production and implications for food safety and public health. Full article

Background/Objectives: The stability and biological safety of newly developed formulations must be established to support their therapeutic efficacy and clinical translation in pulmonary drug delivery. Therefore, this follow-up study comprehensively evaluated the short- and long-term stability and the in vitro cytotoxicity of optimized, co-spray-dried theophylline (THN) dry powders for inhalation against A549 lung epithelial cells. Methods: Two established formulations were selected: THN-RAF (raffinose–leucine–glycine based) and THN-TRE (trehalose–leucine based). Stability was assessed under accelerated conditions (40 °C/75% RH, 3 months) and long-term desiccator storage (25 °C, 1 year) using laser diffraction, SEM, XRPD, FTIR, DSC, TGA, and Andersen Cascade Impaction. As THN-TRE had been previously confirmed to be cytocompatible, only THN-RAF and its components were evaluated against A549 human alveolar epithelial cells using the MTT assay. Results: Under accelerated conditions, both formulations exhibited pronounced recrystallization (Xc up to 89.9%), agglomeration (D [0.9] up to 217.08 µm for THN-TRE), and deterioration in aerodynamic performance (FPF as low as 11.55%, MMAD up to 6.68 µm). By contrast, long-term desiccator storage induced substantial recrystallization (Xc up to 80.7%) while preserving thermal, chemical, and aerodynamic performance (FPF ≈ 40%; MMAD 4.99–5.21 µm). THN-RAF was more resistant to stress-induced agglomeration than THN-TRE. Cytotoxicity assessment confirmed cytocompatibility of THN-RAF, with cell viability exceeding 70.99% at all tested concentrations (up to 500 µg/mL). Conclusions: These findings reveal a marked discrepancy between the outcomes of ICH accelerated testing and long-term desiccator storage. They underscore the importance of considering moisture-protective packaging configurations when designing stability protocols for amorphous inhalable formulations. Full article

Trifoliate orange (Poncirus trifoliata L.), a citrus rootstock valued for its cold hardiness and resistance to broad biotic stress, produces desiccation-sensitive seeds whose successful germination requires optimized pre-sowing treatments for reliable sexual propagation. This study investigated whether chitosan-based coating systems, incorporating glycerol as a plasticizer combined with either an amino acid biostimulant (betaine-proline) or gibberellic acid (GA₃), could improve germination kinetics and seedling establishment. Seeds from mature fruits were subjected to 18 treatments across Petri dish and soil bioassays. In Petri dishes, the germination percentage remained uniformly high (86.67–100%), with GA₃-containing formulations promoting shoot elongation and proline-betaine combinations favoring root development. In soil, treatments primarily modulated germination kinetics: GA₃ combined with chitosan (1.5–2%) increased the germination speed by approximately 3.0–3.4 times relative to the control, while 1.5% chitosan with proline-betaine and glycerol yielded the fastest germination overall. Biomass responses were strongly formulation-dependent: 2% chitosan with GA₃ maximized shoot and total dry weight, whereas 2% chitosan with proline-betaine and glycerol substantially improved root dry biomass and overall seedling quality. Glycerol exerted context-dependent effects, enhancing amino acid formulations while reducing performance in GA₃ mixtures. These findings support the use of optimized chitosan-based treatments as effective tools for accelerating emergence and improving seedling robustness in rootstock production. Full article

Expansive soil is highly susceptible to water-softening and desiccation cracking under alternating wet–dry conditions, often resulting in severe geotechnical and geological hazards. To mitigate these undesirable engineering properties, a composite improvement approach utilizing biochar and sisal fiber was employed. The shear strength and cracking characteristics of the improved expansive soil were systematically investigated through direct shear tests and desiccation cracking tests on specimens prepared with varying biochar contents, sisal fiber contents, and fiber lengths. Scanning electron microscopy (SEM) was further conducted to elucidate the underlying microstructural mechanisms. The results indicated that the individual addition of biochar or sisal fiber enhanced the shear strength of the expansive soil. Increasing the biochar content from 4% to 10% yielded an 8–19% strength gain, whereas raising the sisal fiber content from 1.5‰ to 6‰ led to a more substantial 36–110% improvement. Conversely, extending the fiber length from 10 mm to 30 mm diminished the shear strength by 11–21%. Higher biochar contents progressively increased the internal friction angle (from 14.84° to 18.52°) but were accompanied by a decline in cohesion (from 9.0 kPa to 4.0 kPa). In contrast, increasing the sisal fiber content markedly enhanced cohesion (from 4.7 kPa to 50.3 kPa) while marginally reducing the internal friction angle (from 15.2° to 12.8°). In terms of crack suppression, a 10% biochar content achieved an 86.8% reduction in crack ratio, while 6‰ sisal fiber yielded a 72.4% reduction. Range analysis revealed that crack length and crack ratio were most sensitive to biochar content, whereas crack width was predominantly governed by fiber content. Notably, surface cracking was completely eliminated in the composite specimen prepared with 10% biochar, 4.5‰ sisal fiber, and a fiber length of 20 mm. Microstructural analysis revealed that biochar particles featured rough surfaces and abundant internal pores, while the sisal fibers formed a randomly interwoven network within the soil matrix. The synergistic interplay between the rigid biochar skeleton and the flexible fiber network contributed to the substantial enhancement in both mechanical strength and crack resistance. Full article

Drought stress is one of the most prevalent abiotic stressors and severely impairs plant growth and productivity. Therefore, identifying functional genes associated with drought tolerance is essential for the molecular breeding of drought-resistant crops. The RD22 (Responsive to Desiccation 22) gene family encodes conserved BURP domain-containing proteins that participate in plant responses to drought stress. In this study, two RD22 homologs, DsRD22a and DsRD22b, were isolated and characterized from the drought-tolerant ornamental species Dianthus spiculifolius. Sequence analysis showed that both proteins contain a conserved BURP domain and are typical members of the RD22 family. Tissue-specific expression analysis revealed that both genes were predominantly expressed in leaves and stems. Abiotic stress assays demonstrated that the expression levels of DsRD22a and DsRD22b were significantly induced by abscisic acid (ABA), osmotic stress, and salt stress, whereas their transcriptional responses to relatively low-temperature and oxidative stress were relatively weak. Subcellular localization analysis indicated that DsRD22a and DsRD22b proteins are localized in the cytoplasm. Heterologous overexpression assays showed that transgenic Arabidopsis thaliana lines overexpressing DsRD22a or DsRD22b exhibited significantly enhanced tolerance to salt and osmotic stresses compared with wild-type (WT) plants. Soil drought assays further confirmed that the transgenic lines had higher soluble protein contents and improved drought tolerance than WT plants. These findings suggest that DsRD22a and DsRD22b positively regulate plant responses to drought stress, potentially by promoting soluble protein accumulation. Collectively, DsRD22a and DsRD22b represent valuable candidate genes for the genetic improvement of drought tolerance in plants. Full article

Background: Staphylococcus aureus acquires methicillin resistance genes through the SCCmec element. Although spontaneous SCCmec excision has been observed, its frequency, type-specific variation, and responsiveness to environmental conditions remain undefined. Here, we systematically quantified SCCmec excision across diverse prototypic types/subtypes and evaluated the factors that contribute to excision variability. Methods: Twenty five prototypic MRSA strains (SCCmec types I–VIII, XI–XIII and defined subtypes) were examined under standard growth temperature (37 °C), elevated temperature (42 °C), desiccation, prolonged continuous culture (30 days), and sub-lethal oxacillin pressure. Excision frequencies were quantified using qPCR, normalized to the gyrB housekeeping gene using the formula: 10^{−((Ct,orfX−Ct,gyrB)/3.32)}. Statistical analyses included one-way ANOVA, t-tests, and OLS regression for time-dependent trends. Results: At 37 °C, excision frequencies ranged from 2.40 × 10⁻⁶ to 1.32 × 10⁻³ and varied among representative SCCmec types/subtypes but were unrelated to SCCmec size (R² = 0.027, p = 0.44). Type I showed no detectable excision due to a truncated ccrB gene. At 42 °C, excision increased in 14 of 24 types (median +11.2%; eight significant) and decreased in 10 (median −7.4%; four significant). Desiccation produced similar effects, with nine types increasing (median +7.1%; four significant), 14 decreasing (median −8.2%; five significant), and one unchanged. Continuous culture exhibited progressive increases in excision across multiple types (R² = 0.3–0.94), whereas sub-lethal oxacillin uniformly maintained low detectable excision frequencies across all SCCmec types. Conclusions: Excision varied among representative SCCmec types and was influenced heterogeneously by distinct stress conditions. Continuous culture promoted excision, whereas oxacillin exposure maintained low detectable excision. This work quantitatively confirms spontaneous SCCmec excision and provides new insights into MRSA genome plasticity. Full article

The Aedes aegypti mosquito exhibits a critical behavioral adaptation through its oviposition strategy, laying eggs in dry and wet environments just above the water level, allowing eggs to resist desiccation and hatch only when submerged by rain. To investigate this mechanism, we developed a nonlinear dynamic model incorporating climate-driven parameters affecting egg hatching and adult emergence. Theoretical analysis revealed an imperfect pitchfork bifurcation giving rise to a phenomenon we term basin-mediated hysteresis. Unlike classical hysteresis, which relies on coexisting stable states, this mechanism results from the progressive collapse of the extinction basin boundary. As the control parameter approaches its critical value, the basin of attraction of the trivial equilibrium shrinks. Once the population establishes itself above the threshold, returning the parameter below unity does not restore extinction, leading to an irreversible transition governing population persistence. The model was validated using field data from mosquito traps in a Brazilian city, showing strong agreement with observed seasonal patterns of female captures. Parameters were optimized using the Differential Evolution algorithm, yielding high correlation between model and field data. The results demonstrate that the dual oviposition strategy underlies population persistence and seasonal peaks, providing information for planning interventions amid global arbovirus expansion. Full article

Acinetobacter baumannii is a leading cause of healthcare-associated infections and is classified among the highest-priority antimicrobial-resistant pathogens. Its clinical success reflects the convergence of antimicrobial resistance (AMR) and biological traits that promote environmental persistence and transmission. Acinetobacter baumannii has undergone a remarkable transformation over the past few decades, evolving from a relatively obscure environmental bacterium into a globally recognized multidrug-resistant pathogen. Its prevalence in healthcare settings, particularly intensive care units, has made it a leading cause of ventilator-associated pneumonia, bloodstream infections, wound infections, and urinary tract infections. Beyond its antibiotic resistance, the bacterium’s ability to persist in hospital environments and adapt to host defences has amplified its clinical significance. Recent research has uncovered complex networks of virulence factors, regulatory systems, and metabolic strategies that enable A. baumannii to thrive in hostile environments and evade host immunity, providing new insights into its pathogenesis and potential therapeutic vulnerabilities. This review summarizes the main mechanisms underlying its pathogenicity, including desiccation tolerance, biofilm formation, disinfectant resistance, metal acquisition, motility, and the ability to enter viable but non-culturable states. In A. baumannii, AMR functions as a pathogenesis-adjacent trait, enhancing survival and clonal dissemination through genomic plasticity, resistance islands, efflux systems, and envelope remodeling. Key resistance pathways involve carbapenem-hydrolyzing oxacillinases, metallo-β-lactamases, permeability defects, and multidrug efflux, often coexisting within high-risk clones. From a clinical perspective, management of carbapenem-resistant strains requires accurate infection diagnosis, reliable susceptibility testing, site-specific and PK/PD-optimized therapy, and early reassessment. Overall, the success of A. baumannii reflects the integration of resistance and persistence within healthcare ecosystems, highlighting the need for coordinated strategies combining stewardship, infection control, improved diagnostics, and anti-biofilm or anti-virulence approaches. Full article

Glass ionomer cements (GICs) are considered functionally biomimetic as they participate in ion-exchange processes that partially resemble the behavior of natural enamel and dentin, chemically bond to dental hard tissues, and release fluoride. While GICs are designed to interact with aqueous oral environments, their exposure to dietary beverages may affect their esthetic stability and water-related behavior within the oral environment. For biomimetic restorative materials to perform successfully in the oral environment, they must maintain not only bioactive properties but also esthetic stability and resistance to water-related degradation during exposure to dietary beverages. This study evaluated beverage-induced color changes, water sorption, and water solubility of six GICs following their immersion in coffee, tea, berry juice, cola, and distilled water (n = 5 per material per solution). Color measurements were recorded at baseline and after 2, 4, 6, and 8 weeks using a spectrophotometer, and color change (ΔE) values were calculated using the CIE L*a*b* system. Specimen mass was measured at baseline, after 8 weeks of immersion and then after 4 weeks of desiccation. Data were analyzed using repeated-measures Analysis of Variance (ANOVA) and Fisher’s least significant difference post hoc tests (α = 0.05). The results showed time, material, and solution significantly affected ΔE (p < 0.001). Tea produced the greatest discoloration overall, followed by coffee. ChemFil exhibited the greatest staining susceptibility, while Fuji II showed the lowest staining susceptibility. Water sorption and solubility were material- and solution-dependent. Clinically relevant discoloration of GICs was found when immersed in common beverages over time, with tea showing the strongest staining effect. These findings indicate that although GICs exhibit biomimetic characteristics through their interaction with tooth structures and aqueous environments, their long-term esthetic stability and resistance to environmental challenges should also be considered when selecting restorative materials for clinically visible areas. Full article

Taxillus chinensis (DC.) Danser is an important hemiparasitic medicinal plant whose propagation is severely limited by the desiccation sensitivity of its recalcitrant seeds. Dehydrins (DHNs), which protect plants against dehydration-induced stresses such as salinity, drought, and low temperatures, may play a critical role in protecting recalcitrant seeds. However, the role of DHNs in the seeds of T. chinensis remains unclear. In this study, a differentially expressed gene was identified from the seed transcriptome of T. chinensis and designated TcDHN1. Sequence alignment and phylogenetic analyses revealed that TcDHN1 encodes a dehydrin protein. Heterologous overexpression of TcDHN1 in Arabidopsis did not affect growth under normal conditions. Under salt, drought, and cold stresses, transgenic lines exhibited higher seed germination rates, longer primary roots, and improved seedling growth compared with wild-type (WT) plants. The transgenic lines showed significantly increased activities of antioxidant enzymes, including superoxide dismutase, catalase, and peroxidase. In addition, ectopic overexpression of TcDHN1 in Arabidopsis conferred enhanced tolerance to abiotic stresses compared to WT plants, accompanied by increased expression of the stress-responsive genes Responsive to Desiccation 29A (AtRD29A) and Heat Shock Protein 70-1 (AtHSP70-1). The above results indicate that TcDHN1 confers enhanced tolerance to abiotic stresses. This study provides a functional characterization of an abiotic stress-responsive gene from recalcitrant seeds and identifies a potential genetic resource for molecular breeding. This could potentially improve abiotic stress resistance in T. chinensis and related medicinal plants. Full article

The aetiology of invasive candidiasis is undergoing substantial changes; traditionally, these mycoses have been considered to originate from endogenous reservoirs; however, the increasing prevalence of non-Candida albicans species, such as Candida parapsilosis and Candida auris (also named Candidozyma auris), is a cause of concern as they demonstrate significant exogenous transmission. This challenges the long-standing paradigm of endogenous origin in hospital settings. Unlike previous reviews primarily focused on clinical epidemiology, this work adopts a multidisciplinary perspective combining microbiological evidence with biomaterials science. We analyse how surface roughness, hydrophobicity, and polymer composition within the hospital “plastisphere” influence Candida adhesion and the formation of dry surface biofilms (DSBs). In this specific context, in contrast to C. albicans, primarily associated with mucosal colonisation, C. auris and C. parapsilosis exhibit distinctive adaptations that promote survival in healthcare environments, including pronounced cell surface hydrophobicity and the capacity to form dense cellular aggregates, which facilitate prolonged adherence to synthetic polymers used in medical devices. We also explore the biological mechanisms underlying this resilience, with particular emphasis on the development of dry surface biofilms and viable but non-culturable states. These phenotypic traits confer tolerance to desiccation and resistance to conventional disinfectants, raising concerns that standard hygiene and decontamination protocols may be inadequate to prevent transmission. Understanding these mechanisms is essential for designing effective infection control strategies and mitigating the risk of invasive disease caused by these highly persistent species. Full article

Peroxisomes are dynamic organelles involved in multiple metabolic pathways that respond to cellular and environmental conditions. Yeasts are a useful model for peroxisome studies, as their growth in media containing peroxisome proliferators, such as methanol, induces peroxisome biogenesis. We analyzed Ogataea polymorpha strains defective in peroxisome biogenesis (pex3Δ) or peroxisomal matrix protein import (pex6Δ). The mutant strains differed in their ability to survive dehydration and rehydration after incubation in peroxisome-inducing conditions, but these differences were not related to resistance to oxidative, hyperosmotic, or heat stress. These results indicate that peroxisomes support efficient entry into anhydrobiosis and subsequent recovery through a mechanism that is independent of general stress tolerance. We hypothesized that this effect is mediated by autophagic processes required for the removal of damaged organelles during desiccation. To test this hypothesis, we compared cells with basal peroxisome levels to cells with increased peroxisome numbers following the induction of peroxisome division. Autophagy was inhibited indirectly by disrupting vacuolar acidification with ammonium chloride. This strategy enabled us to explore how the peroxisome abundance and autophagic activity affect the ability of cells to enter anhydrobiosis and survive recovery. Full article

Staphylococcus aureus is an important pathogen that can cause widespread infections as well as severe outbreaks of food poisoning. Recent studies have drawn attention to foodborne pathogens such as S. aureus endowed with the ability to form biofilms and increase resistance to antimicrobial agents as well as environmental stress, posing challenges to food safety. The Clp (caseinolytic protease) protein complex plays a crucial role in energy-dependent protein hydrolysis processes. This mechanism is a common way to maintain intracellular homeostasis and regulation in both prokaryotic and eukaryotic cells, especially under stress conditions. In S. aureus, multiple genes encoding Clp ATPase homologues have been identified: clpC, clpB, clpY, clpX, and clpL. This study investigated the roles of clpC in stress tolerance and biofilm formation of foodborne S. aureus RMSA24 isolated from raw milk. Our results showed that the deletion of the clpC gene significantly reduced the bacterium’s tolerance to heat, desiccation, hydrogen peroxide, and high osmotic pressure compared to wild type (WT). Furthermore, the clpC knockout mutant also exhibited a marked decrease in biofilm formation using Crystal Violet Staining (CVS) and Scanning Electron Microscopy (SEM). Finally, compared to WT, there was a total of 102 DEGs (differentially expressed genes), with a significant downregulation of genes related to biofilm formation (isaA and spa) and heat-shock response (clpP and danJ). These findings suggest that clpC regulates environmental tolerance in S. aureus by modulating the expression of stress- and biofilm-related genes, positioning it as a potential biomarker and a novel target for controlling contamination in the food industry. Full article

Turions (overwintering buds) as modified shoot apices constitute specialized vegetative structures that enable many aquatic vascular plants to withstand adverse environmental conditions such as low temperature, desiccation, or limited light availability. Turions serve as major storage sites for organic reserves, including sugars, proteins, fatty acids, and polyamines. Owing to their high content of energy-rich and nutritionally valuable compounds, turions represent a potential renewable resource for applications in biofuel production, animal feed, and the food industry. We investigated whether arabinogalactan proteins (AGPs) occur in aquatic plant turions and localized these compounds within specific tissues or cell types. This work was designed to evaluate whether stress-resistant storage organs may constitute a practical reservoir of AGPs. Considering the central role of AGPs in plant responses to abiotic stress, we hypothesized that turions, which routinely encounter cold, anoxia, and intermittent dehydration, would exhibit particularly high AGP accumulation. Mature turions of aquatic species (Aldrovanda vesiculosa, Utricularia australis, U. intermedia, and Caldesia parnassifolia) were used. Immunofluorescent labeling with AGP-specific antibodies (JIM8, JIM13, JIM14, LM2, MAC207) and confocal laser scanning microscopy were employed. In Aldrovanda vesiculosa and Caldesia parnassifolia, AGP epitopes were abundantly presented in cytoplasmic compartments. AGP epitopes occurred in secretory structures in turions of all examined species (trichomes of Aldrovanda and Utricularia, secretory ducts of Caldesia). In analyzing turions of four different species, we identified Aldrovanda vesiculosa turions as the most promising potential source of AGPs, also noting their high reserve potential for use in animal feed or the food industry. Full article