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20 pages, 2299 KiB  
Article
Valorization of Waste Mineral Wool and Low-Rank Peat in the Fertilizer Industry in the Context of a Resource-Efficient Circular Economy
by Marta Huculak-Mączka, Dominik Nieweś, Kinga Marecka and Magdalena Braun-Giwerska
Sustainability 2025, 17(15), 7083; https://doi.org/10.3390/su17157083 (registering DOI) - 5 Aug 2025
Abstract
This study aims to evaluate eco-innovative solutions in the fertilizer industry that allow for waste valorization in the context of a resource-efficient circular economy. A comprehensive reuse strategy was developed for low-rank peat and post-cultivation horticultural mineral wool, involving the extraction of valuable [...] Read more.
This study aims to evaluate eco-innovative solutions in the fertilizer industry that allow for waste valorization in the context of a resource-efficient circular economy. A comprehensive reuse strategy was developed for low-rank peat and post-cultivation horticultural mineral wool, involving the extraction of valuable humic substances from peat and residual nutrients from used mineral wool, followed by the use of both post-extraction residues to produce organic–mineral substrates. The resulting products/semifinished products were characterized in terms of their composition and properties, which met the requirements necessary to obtain the admission of this type of product to the market in accordance with the Regulation of the Minister for Agriculture and Rural Development of 18 June 2008 on the implementation of certain provisions of the Act on fertilizers and fertilization (Journal of Laws No 119, item 765). Elemental analysis, FTIR spectroscopy, and solid-state CP-MAS 13C NMR spectroscopy suggest that post-extraction peat has a relatively condensed structure with a high C content (47.4%) and a reduced O/C atomic ratio and is rich in alkyl-like matter (63.2%) but devoid of some functional groups in favor of extracted fulvic acids. Therefore, it remains a valuable organic biowaste, which, in combination with post-extraction waste mineral wool in a ratio of 60:40 and possibly the addition of mineral nutrients, allows us to obtain a completely new substrate with a bulk density of 264 g/m3, a salinity of 7.8 g/dm3 and a pH of 5.3, with an appropriate content of heavy metals and with no impurities, meeting the requirements of this type of product. A liquid fertilizer based on an extract containing previously recovered nutrients also meets the criteria in terms of quality and content of impurities and can potentially be used as a fertilizing product suitable for agricultural crops. This study demonstrates a feasible pathway for transforming specific waste streams into valuable agricultural inputs, contributing to environmental protection and sustainable production. The production of a new liquid fertilizer using nutrients recovered from post-cultivation mineral wool and the preparation of an organic–mineral substrate using post-extraction solid residue is a rational strategy for recycling hard-to-biodegrade end-of-life products. Full article
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18 pages, 7363 KiB  
Article
Agronomic Evaluation of Compost Formulations Based on Mining Tailings and Microbial Mats from Geothermal Sources
by María Jesús Puy-Alquiza, Miren Yosune Miranda Puy, Raúl Miranda-Avilés, Pooja Vinod Kshirsagar and Cristina Daniela Moncada Sanchez
Recycling 2025, 10(4), 156; https://doi.org/10.3390/recycling10040156 - 5 Aug 2025
Abstract
This study, conducted in Mexico, evaluates the agricultural potential of three compost formulations BFS1, BFS2, and BFS3 produced from mining tailings and thermophilic microbial mats and collected from geothermal environments. The physicochemical characterization included pH, electrical conductivity (EC), macronutrients (N, P, K, Ca, [...] Read more.
This study, conducted in Mexico, evaluates the agricultural potential of three compost formulations BFS1, BFS2, and BFS3 produced from mining tailings and thermophilic microbial mats and collected from geothermal environments. The physicochemical characterization included pH, electrical conductivity (EC), macronutrients (N, P, K, Ca, Mg, and S), micronutrients (Fe, Zn, B, Cu, Mn, Mo, and Ni), organic matter (OM), and the carbon-to-nitrogen (C/N) ratio. All composts exhibited neutral pH values (7.38–7.52), high OM content (38.5–48.4%), and optimal C/N ratios (10.5–13.9), indicating maturity and chemical stability. Nitrogen ranged from 19 to 21 kg·t−1, while potassium and calcium were present in concentrations beneficial for crop development. However, EC values (3.43–3.66 dS/m) and boron levels (>160 ppm) were moderately high, requiring caution in saline soils or with boron-sensitive crops. A semi-quantitative Compost Quality Index (CQI) ranked BFS3 highest due to elevated OM and potassium content, followed by BFS1. BFS2, while rich in nitrogen, scored lower due to excessive boron. One-way ANOVA revealed no significant difference in nitrogen (p > 0.05), but it did reveal significant differences in potassium (p < 0.01) and boron (p < 0.001) among formulations. These results confirm the potential of mining tailings—microbial mat composts are low-cost, nutrient-rich biofertilizers. They are suitable for field crops or as components in nursery substrates, particularly when EC and boron are managed through dilution. This study promotes the circular reuse of geothermal and industrial residues and contributes to sustainable soil restoration practices in mining-affected regions through innovative composting strategies. Full article
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18 pages, 2082 KiB  
Article
Insect Assemblage and Insect–Plant Relationships in a Cultivated Guayule (Parthenium argentatum A. Gray) Plot in Spain
by Eduardo Jarillo, Guayente Latorre, Enrique Fernández-Carrillo, Sara Rodrigo-Gómez, José Luis Yela and Manuel Carmona
Insects 2025, 16(8), 808; https://doi.org/10.3390/insects16080808 (registering DOI) - 4 Aug 2025
Abstract
This study aims to characterize for the first time the insect assemblage associated with sown, introduced guayule (Parthenium argentatum A. Gray, Asteraceae) in Castilla-La Mancha, Spain, and identify potential relationships with the crop. Insect sampling was conducted using nets and pan traps [...] Read more.
This study aims to characterize for the first time the insect assemblage associated with sown, introduced guayule (Parthenium argentatum A. Gray, Asteraceae) in Castilla-La Mancha, Spain, and identify potential relationships with the crop. Insect sampling was conducted using nets and pan traps during spring and early summer, coinciding with the flowering period of the plant. A total of 352 insect species/morphospecies across 12 orders were identified. Diptera, Coleoptera, Hemiptera, and Hymenoptera were the most species-rich and abundant orders. Within these orders, Muscidae, Syrphidae, Tenebrionidae, Dermestidae, Miridae, Halictidae, and Apidae were the most numerous families. Guayule flowering intensity increased gradually until mid-June, aligning with the peak activity of pollinating Diptera. The majority of the identified insects (74.4%) were potential pollinators, while nearly 50% were detritivores and approximately 30% were herbivorous. The similarity in insect families and functional roles observed in this study to previous studies in the USA and Mexico suggest that guayule may serve as a similar trophic resource for insects in Spain, despite being a non-native species. Full article
(This article belongs to the Section Insect Ecology, Diversity and Conservation)
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15 pages, 1685 KiB  
Article
Wildfires and Palm Species Response in a Terra Firme Amazonian Social Forest
by Tinayra T. A. Costa, Vynicius B. Oliveira, Maria Fabíola Barros, Fernando W. C. Andrade, Marcelo Tabarelli and Ima C. G. Vieira
Forests 2025, 16(8), 1271; https://doi.org/10.3390/f16081271 - 3 Aug 2025
Viewed by 135
Abstract
Tropical forests continue to experience high levels of habitat loss and degradation, with wildfires becoming a frequent component of human-modified landscapes. Here we investigate the response of palm species to the conversion of old-growth forests to successional mosaics, including forest patches burned during [...] Read more.
Tropical forests continue to experience high levels of habitat loss and degradation, with wildfires becoming a frequent component of human-modified landscapes. Here we investigate the response of palm species to the conversion of old-growth forests to successional mosaics, including forest patches burned during wildfires. Palms (≥50 cm height) were recorded once in 2023–2024, across four habitat classes: terra firme old-growth stands, regenerating forest stands associated with slash-and-burn agriculture, old-growth stands burned once and twice, and active cassava fields, in the Tapajós-Arapiuns Extractive Reserve, in the eastern Brazilian Amazon. The flammability of palm leaf litter and forest litter were also examined to assess the potential connections between palm proliferation and wildfires. A total of 10 palm species were recorded in this social forest (including slash-and-burn agriculture and resulting successional mosaics), with positive, negative, and neutral responses to land use. Species richness did not differ among forest habitats, but absolute palm abundance was greatest in disturbed habitats. Only Attalea spectabilis Mart. (curuá) exhibited increased relative abundance across disturbed habitats, including active cassava field. Attalea spectabilis accounted for almost 43% of all stems in the old-growth forest, 89% in regenerating forests, 90% in burned forests, and 79% in crop fields. Disturbed habitats supported a five-to-ten-fold increment in curuá leaves as a measure of habitat flammability. Although curuá litter exhibited lower flame temperature and height, its lower carbon and higher volatile content is expected to be more sensitive to fire ignition and promote the spread of wildfires. The conversion of old-growth forests into social forests promotes the establishment of palm-dominated forests, increasing the potential for a forest transition further fueled by wildfires, with effects on forest resilience and social reproduction still to be understood. Full article
(This article belongs to the Special Issue Ecosystem-Disturbance Interactions in Forests)
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17 pages, 3038 KiB  
Article
Neighbor Relatedness Contributes to Improvement in Grain Yields in Rice Cultivar Mixtures
by You Xu, Qin-Hang Han, Shuai-Shuai Xie and Chui-Hua Kong
Plants 2025, 14(15), 2385; https://doi.org/10.3390/plants14152385 - 2 Aug 2025
Viewed by 222
Abstract
The improvement in yield in cultivar mixtures has been well established. Despite increasing knowledge of the improvement involving within-species diversification and resource use efficiency, little is known about the benefits arising from relatedness-mediated intraspecific interactions in cultivar mixtures. This study used a relatedness [...] Read more.
The improvement in yield in cultivar mixtures has been well established. Despite increasing knowledge of the improvement involving within-species diversification and resource use efficiency, little is known about the benefits arising from relatedness-mediated intraspecific interactions in cultivar mixtures. This study used a relatedness gradient of rice cultivars to test whether neighbor relatedness contributes to improvements in grain yields in cultivar mixtures. We experimentally demonstrated the grain yield of rice cultivar mixtures with varying genetic relatedness under both field and controlled conditions. As a result, a closely related cultivar mixture had increased grain yield compared to monoculture and distantly related mixtures by optimizing the root-to-shoot ratio and accelerating flowering. The benefits over monoculture were most pronounced when compared to the significant yield reductions observed in distantly related mixtures. The relatedness-mediated improvement in yields depended on soil volume and nitrogen use level, with effects attenuating under larger soil volumes or nitrogen deficiency. Furthermore, neighbor relatedness enhanced the richness and diversity of both bacterial and fungal communities in the rhizosphere soil, leading to a significant restructuring of the microbial community composition. These findings suggest that neighbor relatedness may improve the grain yield of rice cultivar mixtures. Beneficial plant–plant interactions may be generated by manipulating cultivar kinship within a crop species. A thorough understanding of kinship strategies in cultivar mixtures offers promising prospects for increasing crop production. Full article
(This article belongs to the Special Issue Plant Chemical Ecology—2nd Edition)
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25 pages, 6358 KiB  
Article
First Assessment of the Biodiversity of True Slime Molds in Swamp Forest Stands of the Knyszyn Forest (Northeast Poland) Using the Moist Chambers Detection Method
by Tomasz Pawłowicz, Igor Żebrowski, Gabriel Michał Micewicz, Monika Puchlik, Konrad Wilamowski, Krzysztof Sztabkowski and Tomasz Oszako
Forests 2025, 16(8), 1259; https://doi.org/10.3390/f16081259 - 1 Aug 2025
Viewed by 122
Abstract
True slime molds (Eumycetozoa) remain under-explored globally, particularly in water-logged forest habitats. Despite evidence suggesting a high biodiversity potential in the Knyszyn Forest of north-eastern Poland, no systematic effort had previously been undertaken there. In the present survey, plant substrates from [...] Read more.
True slime molds (Eumycetozoa) remain under-explored globally, particularly in water-logged forest habitats. Despite evidence suggesting a high biodiversity potential in the Knyszyn Forest of north-eastern Poland, no systematic effort had previously been undertaken there. In the present survey, plant substrates from eight swampy sub-compartments were incubated for over four months, resulting in the detection of fifteen slime mold species. Four of these taxa are newly reported for northern and north-eastern Poland, while several have been recorded only a handful of times in the global literature. These findings underscore how damp, nutrient-rich conditions foster Eumycetozoa and demonstrate the effectiveness of moist-chamber culturing in revealing rare or overlooked taxa. Current evidence shows that, although slime molds may occasionally colonize living plant or fungal tissues, their influence on crop productivity and tree vitality is negligible; they are therefore better regarded as biodiversity indicators than as pathogens or pests. By establishing a replicable framework for studying water-logged environments worldwide, this work highlights the ecological importance of swamp forests in sustaining microbial and slime mold diversity. Full article
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17 pages, 587 KiB  
Review
Exploring the Potential of Biochar in Enhancing U.S. Agriculture
by Saman Janaranjana Herath Bandara
Reg. Sci. Environ. Econ. 2025, 2(3), 23; https://doi.org/10.3390/rsee2030023 - 1 Aug 2025
Viewed by 141
Abstract
Biochar, a carbon-rich material derived from biomass, presents a sustainable solution to several pressing challenges in U.S. agriculture, including soil degradation, carbon emissions, and waste management. Despite global advancements, the U.S. biochar market remains underexplored in terms of economic viability, adoption potential, and [...] Read more.
Biochar, a carbon-rich material derived from biomass, presents a sustainable solution to several pressing challenges in U.S. agriculture, including soil degradation, carbon emissions, and waste management. Despite global advancements, the U.S. biochar market remains underexplored in terms of economic viability, adoption potential, and sector-specific applications. This narrative review synthesizes two decades of literature to examine biochar’s applications, production methods, and market dynamics, with a focus on its economic and environmental role within the United States. The review identifies biochar’s multifunctional benefits: enhancing soil fertility and crop productivity, sequestering carbon, reducing greenhouse gas emissions, and improving water quality. Recent empirical studies also highlight biochar’s economic feasibility across global contexts, with yield increases of up to 294% and net returns exceeding USD 5000 per hectare in optimized systems. Economically, the global biochar market grew from USD 156.4 million in 2021 to USD 610.3 million in 2023, with U.S. production reaching ~50,000 metric tons annually and a market value of USD 203.4 million in 2022. Forecasts project U.S. market growth at a CAGR of 11.3%, reaching USD 478.5 million by 2030. California leads domestic adoption due to favorable policy and biomass availability. However, barriers such as inconsistent quality standards, limited awareness, high costs, and policy gaps constrain growth. This study goes beyond the existing literature by integrating market analysis, SWOT assessment, cost–benefit findings, and production technologies to highlight strategies for scaling biochar adoption. It concludes that with supportive legislation, investment in research, and enhanced supply chain transparency, biochar could become a pivotal tool for sustainable development in the U.S. agricultural and environmental sectors. Full article
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18 pages, 1618 KiB  
Article
Native Grass Enhances Bird, Dragonfly, Butterfly and Plant Biodiversity Relative to Conventional Crops in Midwest, USA
by Steven I. Apfelbaum, Susan M. Lehnhardt, Michael Boston, Lea Daly, Gavin Pinnow, Kris Gillespie and Donald M. Waller
Agriculture 2025, 15(15), 1666; https://doi.org/10.3390/agriculture15151666 - 1 Aug 2025
Viewed by 162
Abstract
Conspicuous declines in native grassland habitats have triggered sharp reductions in grassland birds, dragonflies, butterflies, and native plant populations and diversity. We compared these biotic groups among three crop type treatments: corn, alfalfa, and a perennial native grass, Virginia wild rye, (Elymus [...] Read more.
Conspicuous declines in native grassland habitats have triggered sharp reductions in grassland birds, dragonflies, butterflies, and native plant populations and diversity. We compared these biotic groups among three crop type treatments: corn, alfalfa, and a perennial native grass, Virginia wild rye, (Elymus virginicus L.) or VWR. This crop type had 2-3X higher bird, dragonfly, butterfly and plant species richness, diversity, and faunal abundance relative to alfalfa and corn types. VWR crop fields also support more obligate grassland bird species and higher populations of dragonfly and butterfly species associated with grasslands and wet meadows. In contrast, the corn and alfalfa types support few or no obligatory grassland birds and mostly non-native insects such as the white cabbage looper (Artogeia rapae L.), the common yellow sulfur butterfly (Colias philodice Godart.), and the mobile and migratory common green darner dragonfly (Anax junius Drury.). In sum, the VWR perennial native grass crop type offers a special opportunity to improve the diversity and abundance of grassland bird species, beneficial insect species, and many native plant species within agricultural landscapes. Full article
(This article belongs to the Section Agricultural Systems and Management)
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23 pages, 4379 KiB  
Article
Large Vision Language Model: Enhanced-RSCLIP with Exemplar-Image Prompting for Uncommon Object Detection in Satellite Imagery
by Taiwo Efunogbon, Abimbola Efunogbon, Enjie Liu, Dayou Li and Renxi Qiu
Electronics 2025, 14(15), 3071; https://doi.org/10.3390/electronics14153071 - 31 Jul 2025
Viewed by 142
Abstract
Large Vision Language Models (LVLMs) have shown promise in remote sensing applications, yet struggle with “uncommon” objects that lack sufficient public labeled data. This paper presents Enhanced-RSCLIP, a novel dual-prompt architecture that combines text prompting with exemplar-image processing for cattle herd detection in [...] Read more.
Large Vision Language Models (LVLMs) have shown promise in remote sensing applications, yet struggle with “uncommon” objects that lack sufficient public labeled data. This paper presents Enhanced-RSCLIP, a novel dual-prompt architecture that combines text prompting with exemplar-image processing for cattle herd detection in satellite imagery. Our approach introduces a key innovation where an exemplar-image preprocessing module using crop-based or attention-based algorithms extracts focused object features which are fed as a dual stream to a contrastive learning framework that fuses textual descriptions with visual exemplar embeddings. We evaluated our method on a custom dataset of 260 satellite images across UK and Nigerian regions. Enhanced-RSCLIP with crop-based exemplar processing achieved 72% accuracy in cattle detection and 56.2% overall accuracy on cross-domain transfer tasks, significantly outperforming text-only CLIP (31% overall accuracy). The dual-prompt architecture enables effective few-shot learning and cross-regional transfer from data-rich (UK) to data-sparse (Nigeria) environments, demonstrating a 41% improvement over baseline approaches for uncommon object detection in satellite imagery. Full article
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17 pages, 1160 KiB  
Article
Enhanced Antioxidant and Antiproliferative Activities of Apple and Korean Green Chili Pepper Extracts Cultivated with Mineral Supplementation
by Ji-Sun Lim, Mi-Hee Yu, Dong Kyu Choi, Hae Won Kim, Seung-Hwan Park, Sin-Il Sin and Jong-Sang Kim
Foods 2025, 14(15), 2685; https://doi.org/10.3390/foods14152685 - 30 Jul 2025
Viewed by 213
Abstract
Apples and Korean green chili peppers are rich in phytochemicals and recognized for their diverse bioactive properties. Given the potential to enhance these beneficial compounds, this study investigated the effects of mineral supplementation during cultivation on the antioxidant and antiproliferative activities of extracts [...] Read more.
Apples and Korean green chili peppers are rich in phytochemicals and recognized for their diverse bioactive properties. Given the potential to enhance these beneficial compounds, this study investigated the effects of mineral supplementation during cultivation on the antioxidant and antiproliferative activities of extracts from both crops. Mineral-enriched cultivation significantly increased the total phenolic and flavonoid contents in both crops, which was accompanied by enhanced DPPH and ABTS radical scavenging activities. Moreover, the mineral-supplemented extracts of Korean green chili pepper activated the Nrf2 signaling pathway and upregulated downstream antioxidant enzymes, including heme oxygenase-1 (HO-1), γ-glutamylcysteine ligase (GCL), and glutathione peroxidase (GPx). Notably, the mineral-supplemented Korean green chili pepper extract significantly suppressed the proliferation of human colorectal cancer cells. These findings suggest that mineral supplementation during cultivation may improve the functional quality of apples and Korean green chili peppers, supporting their potential application in cancer prevention and complementary therapeutic strategies. Full article
(This article belongs to the Special Issue Bioactive Phenolic Compounds from Agri-Food and Its Wastes)
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32 pages, 1770 KiB  
Article
Regional Patterns in Weed Composition of Maize Fields in Eastern Hungary: The Balance of Environmental and Agricultural Factors
by Mihály Zalai, Erzsébet Tóth, János György Nagy and Zita Dorner
Agronomy 2025, 15(8), 1814; https://doi.org/10.3390/agronomy15081814 - 26 Jul 2025
Viewed by 437
Abstract
The primary aim of this study was to explore the influence of abiotic factors on weed development in maize fields, with the goal of informing more effective weed management practices. We focused on identifying key environmental, edaphic, and agricultural variables that contribute to [...] Read more.
The primary aim of this study was to explore the influence of abiotic factors on weed development in maize fields, with the goal of informing more effective weed management practices. We focused on identifying key environmental, edaphic, and agricultural variables that contribute to weed infestations, particularly before the application of spring herbicide treatments. Field investigations were conducted from 2018 to 2021 across selected maize-growing regions in Hungary. Over the four-year period, a total of 51 weed species were recorded, with Echinochloa crus-galli, Chenopodium album, Portulaca oleracea, and Hibiscus trionum emerging as the most prevalent taxa. Collectively, these four species accounted for more than half (52%) of the total weed cover. Altogether, the 20 most dominant species contributed 95% of the overall weed coverage. The analysis revealed that weed cover, species richness, and weed diversity were significantly affected by soil properties, nutrient levels, geographic location, and tillage systems. The results confirm that the composition of weed species was influenced by several environmental and management-related factors, including soil parameters, geographical location, annual precipitation, tillage method, and fertilizer application. Environmental factors collectively explained a slightly higher proportion of the variance (13.37%) than farming factors (12.66%) at a 90% significance level. Seasonal dynamics and crop rotation history also played a notable role in species distribution. Nutrient inputs, particularly nitrogen, phosphorus, and potassium, influenced both species diversity and floristic composition. Deep tillage practices favored the proliferation of perennial species, whereas shallow cultivation tended to promote annual weeds. Overall, the composition of weed vegetation proved to be a valuable indicator of site-specific soil conditions and agricultural practices. These findings underscore the need to tailor weed management strategies to local environmental and soil contexts for sustainable crop production. Full article
(This article belongs to the Special Issue State-of-the-Art Research on Weed Populations and Community Dynamics)
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19 pages, 788 KiB  
Review
Advances in Genetic Diversity of Germplasm Resources, Origin and Evolution of Turnip Rape (Brassica rapa L.)
by Xiaoming Lu, Tianyu Zhang, Yuanqiang Ma, Chunyang Han, Wenxin Yang, Yuanyuan Pu, Li Ma, Junyan Wu, Gang Yang, Wangtian Wang, Tingting Fan, Lijun Liu and Wancang Sun
Plants 2025, 14(15), 2311; https://doi.org/10.3390/plants14152311 - 26 Jul 2025
Viewed by 234
Abstract
During a prolonged domestication and environmental selection, Brassica rapa has formed diverse morphological types during a cultivation process of up to 8000 years, such as root-type turnips (Brassica rapa var. rapa), leaf-type Chinese cabbage (Brassica rapa var. pekinensis), oil-type [...] Read more.
During a prolonged domestication and environmental selection, Brassica rapa has formed diverse morphological types during a cultivation process of up to 8000 years, such as root-type turnips (Brassica rapa var. rapa), leaf-type Chinese cabbage (Brassica rapa var. pekinensis), oil-type rapeseed (Brassica rapa L.), and other rich types. China is one of the origins of Brassica rapa L., which is spread all over the east, west, south, and north of China. Studying its origin and evolution holds significant importance for unraveling the cultivation history of Chinese oilseed crops, intraspecific evolutionary relationships, and the utilization value of genetic resources. This article summarizes the cultivation history, evolution, classification research progress, and germplasm resource diversity of Brassica rapa var. oleifera in China. Combining karyotype analysis, genomic information, and wild relatives of Brassica rapa var. oleifera discovered on the Qinghai–Tibet Plateau, it is proposed that Brassica rapa var. oleifera has the characteristic of polycentric origin, and Gansu Province in China is one of the earliest regions for its cultivation. Brassica rapa var. oleifera, originating from the Mediterranean region, was diffused to the East Asian continent through two independent transmission paths (one via the Turkish Plateau and the other via Central Asia and Siberia). Analyzing the genetic diversity characteristics and evolutionary trajectories of these two transmission paths lays a foundation for clarifying the origin and evolutionary process of Brassica rapa var. oleifera and accelerating the breeding of Brassica rapa var. oleifera in China. Despite existing research on the origin of Brassica rapa L., the domestication process of this species remains unresolved. Future studies will employ whole-genome resequencing to address this fundamental question. Full article
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20 pages, 2181 KiB  
Article
Metabarcoding Analysis Reveals Microbial Diversity and Potential Soilborne Pathogens Associated with Almond Dieback and Decline
by André Albuquerque, Mariana Patanita, Joana Amaro Ribeiro, Maria Doroteia Campos, Filipa Santos, Tomás Monteiro, Margarida Basaloco and Maria do Rosário Félix
Plants 2025, 14(15), 2309; https://doi.org/10.3390/plants14152309 - 26 Jul 2025
Viewed by 389
Abstract
Almond decline and dieback have become significant challenges in newly established orchards, with symptoms including internal necrosis, canker, and external gummosis. This work aims to explore the potential fungal and bacterial causative agents through metabarcoding and traditional culture plate isolation across six almond [...] Read more.
Almond decline and dieback have become significant challenges in newly established orchards, with symptoms including internal necrosis, canker, and external gummosis. This work aims to explore the potential fungal and bacterial causative agents through metabarcoding and traditional culture plate isolation across six almond cultivars. Our results emphasize the multifactorial nature of almond decline and dieback, with possible co-infections by opportunistic fungi and bacteria playing a central role. Classical isolation identified 47 fungal species or genera, including Diaporthe amygdali, Diplodia corticola, Phytophthora sp., and several Fusarium species. Almond metabarcoding revealed a more diverse microbial community, highlighting the prevalence of soilborne pathogens such as Neocosmospora rubicola, Dactylonectria estremocensis, and Plectosphaerella niemeijerarum. Soil metabarcoding suggested that these pathogens likely originate from nursery substrates or soils shared with other crops, such as olives and vineyards, that serve as a source of inoculum. ‘Soleta’ generally presented lower richness when compared to the other tested cultivars, suggesting a higher degree of biotic stress and decreased plant resilience. This study highlights the value of integrating NGS approaches to comprehensively study complex diseases and the need for further research on pathogen interactions and cultivar susceptibility for the future development of new sustainable, targeted management strategies in almond orchards. Full article
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28 pages, 2549 KiB  
Article
A 25K Wheat SNP Array Revealed the Genetic Diversity and Population Structure of Durum Wheat (Triticum turgidum subsp. durum) Landraces and Cultivars
by Lalise Ararsa, Behailu Mulugeta, Endashaw Bekele, Negash Geleta, Kibrom B. Abreha and Mulatu Geleta
Int. J. Mol. Sci. 2025, 26(15), 7220; https://doi.org/10.3390/ijms26157220 - 25 Jul 2025
Viewed by 1154
Abstract
Durum wheat, the world’s second most cultivated wheat species, is a staple crop, critical for global food security, including in Ethiopia where it serves as a center of diversity. However, climate change and genetic erosion threaten its genetic resources, necessitating genomic studies to [...] Read more.
Durum wheat, the world’s second most cultivated wheat species, is a staple crop, critical for global food security, including in Ethiopia where it serves as a center of diversity. However, climate change and genetic erosion threaten its genetic resources, necessitating genomic studies to support conservation and breeding efforts. This study characterized genome-wide diversity, population structure (STRUCTURE, principal coordinate analysis (PCoA), neighbor-joining trees, analysis of molecular variance (AMOVA)), and selection signatures (FST, Hardy–Weinberg deviations) in Ethiopian durum wheat by analyzing 376 genotypes (148 accessions) using an Illumina Infinium 25K single nucleotide polymorphism (SNP) array. A set of 7842 high-quality SNPs enabled the assessments, comparing landraces with cultivars and breeding populations. Results revealed moderate genetic diversity (mean polymorphism information content (PIC) = 0.17; gene diversity = 0.20) and identified 26 loci under selection, associated with key traits like grain yield, stress tolerance, and disease resistance. AMOVA revealed 80.1% variation among accessions, with no significant differentiation by altitude, region, or spike density. Landraces formed distinct clusters, harboring unique alleles, while admixture suggested gene flow via informal seed exchange. The findings highlight Ethiopia’s rich durum wheat diversity, emphasizing landraces as reservoirs of adaptive alleles for breeding. This study provides genomic insights to guide conservation and the development of climate-resilient cultivars, supporting sustainable wheat production globally. Full article
(This article belongs to the Special Issue Latest Research on Plant Genomics and Genome Editing, 2nd Edition)
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15 pages, 1328 KiB  
Article
Effects of Ridge-Furrow Film Mulching Patterns on Soil Bacterial Diversity in a Continuous Potato Cropping System
by Shujuan Jiao, Yichen Kang, Weina Zhang, Yuhui Liu, Hong Li, Wenlin Li and Shuhao Qin
Agronomy 2025, 15(8), 1784; https://doi.org/10.3390/agronomy15081784 - 24 Jul 2025
Viewed by 226
Abstract
Soil bacteria drive biogeochemical cycles and influence disease suppression, playing pivotal roles in sustainable agriculture. Using Illumina MiSeq sequencing, we assessed how six ridge-furrow film mulching patterns affect soil bacterial diversity in a continuous potato system. The Shannon index showed significantly higher diversity [...] Read more.
Soil bacteria drive biogeochemical cycles and influence disease suppression, playing pivotal roles in sustainable agriculture. Using Illumina MiSeq sequencing, we assessed how six ridge-furrow film mulching patterns affect soil bacterial diversity in a continuous potato system. The Shannon index showed significantly higher diversity in fully mulched treatments (T2–T3) versus controls (CK), suggesting mulching enhances microbial community richness. This result suggests that complete mulching combined with ridge planting (T2) may significantly enhance bacterial proliferation in soil. The bacterial communities were predominantly composed of Acidobacteria, Pseudomonadota, Bacteroidota, Chloroflexota, and Planctomycetota. Among these, Acidobacteria showed the highest abundance, with ridge planting patterns favoring greater Acidobacteria richness compared to furrow planting. In contrast, Pseudomonadota exhibited higher abundance under half-mulching conditions than under complete mulching. At class level, Acidobacteria and Proteobacteria emerged as the most abundant groups, with Proteobacteria constituting 22.6–35.7% of total microbial populations. Notably, Proteobacteria demonstrated particular dominance under the complete mulching with ridge planting pattern (T2). At the genus level, Subgroup_6_norank represented the most dominant taxon among the 439 identified bacterial genera, accounting for 14.0–20.2% of communities across all treatments, with half-mulching ridge planting (T4) showing the highest relative abundance. Our findings demonstrate that different ridge-furrow film mulching patterns significantly influence soil microbial diversity. While traditional non-mulched (CK) and mulched flat plots (T1) exhibited similar impacts on bacterial community structure, other treatments displayed distinct taxonomic profiles. Complete mulching patterns, particularly ridge planting (T2), appear most conducive to microbial development, suggesting their potential to enhance soil biogeochemical cycling in continuous cropping systems. These results provide valuable insights for optimizing mulching practices to improve soil health in agricultural ecosystems. Full article
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