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25 pages, 8466 KiB  
Article
Influence on Existing Underlying Metro Tunnel Deformation from Small Clear-Distance Rectangular Box Jacking: Monitoring and Simulation
by Chong Ma, Hao Zhou and Baosong Ma
Buildings 2025, 15(14), 2547; https://doi.org/10.3390/buildings15142547 - 19 Jul 2025
Viewed by 278
Abstract
Rectangular box jacking is widely used in densely developed urban areas. However, when conducted with limited clear distance near existing metro tunnels, it introduces considerable structural safety risks. This study investigates a large-section rectangular box jacking project in Suzhou that crosses a double-line [...] Read more.
Rectangular box jacking is widely used in densely developed urban areas. However, when conducted with limited clear distance near existing metro tunnels, it introduces considerable structural safety risks. This study investigates a large-section rectangular box jacking project in Suzhou that crosses a double-line metro tunnel with minimal vertical clear distance. Integrated field monitoring and finite element simulations were conducted to analyze the tunnel’s deformation behavior during various jacking phases. The results show that the upline tunnel experienced greater uplift than the downline tunnel, with maximum vertical displacement occurring directly beneath the jacking axis. The affected zone extended approximately 20 m beyond the pipe gallery boundaries. Both the tunnel vault and ballast bed exhibited vertical uplift, while the hance displaced laterally toward the launching shaft. These deformations showed clear stage-dependent patterns strongly influenced by the relative position of the jacking machine. Numerical simulations demonstrated that doubling the pipe–tunnel clearance reduced the vault displacement by 58.87% (upline) and 51.95% (downline). Increasing the pipe–slurry friction coefficient from 0.1 to 0.3 caused the hance displacement difference to rise from 0.12 mm to 0.36 mm. Further sensitivity analysis reveals that when the jacking machine is positioned directly above the tunnel, grouting pressure is the greatest influence on the structural response and must be carefully controlled. The proposed methodology and findings offer valuable insights for future applications in similar tunnelling projects. Full article
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13 pages, 7320 KiB  
Article
Determination of Main Bearing Dynamic Clearance in a Shield Tunneling Machine Through a Broadband PMUT Array with a Decreased Blind Area and High Accuracy
by Guoxi Luo, Haoyu Zhang, Delai Liu, Wenyan Li, Min Li, Zhikang Li, Lin Sun, Ping Yang, Ryutaro Maeda and Libo Zhao
Sensors 2025, 25(13), 4182; https://doi.org/10.3390/s25134182 - 4 Jul 2025
Cited by 1 | Viewed by 338
Abstract
Traditional PMUT ultrasonic ranging systems usually possess a large measurement blind area under the integrated transmit–receive mode, dramatically limiting its distance measurement in confined spaces, such as when determining the clearance of large bearing components. Here, a broadband PMUT rangefinder was designed by [...] Read more.
Traditional PMUT ultrasonic ranging systems usually possess a large measurement blind area under the integrated transmit–receive mode, dramatically limiting its distance measurement in confined spaces, such as when determining the clearance of large bearing components. Here, a broadband PMUT rangefinder was designed by integrating six types of different cells with adjacent resonant frequencies into an array. Through overlapping and coupling of the bandwidths from the different cells, the proposed PMUTs showed a wide –6 dB fractional bandwidth of 108% in silicon oil. Due to the broadening of bandwidth, the device could obtain the maximum steady state with less excitation (5 cycles versus 14 cycles) and reduce its residual ring-down (ca. 6 μs versus 15 μs) compared with the traditional PMUT array with the same cells, resulting in a small blind area. The pulse–echo ranging experiments demonstrated that the blind area was effectively reduced to 4.4 mm in air or 12.8 mm in silicon oil, and the error was controlled within ±0.3 mm for distance measurements up to 250 mm. In addition, a specific ultrasound signal processing circuit with functions of transmitting, receiving, and processing ultrasonic waves was developed. Combining the processing circuit and PMUT device, the system was applied to determine the axial clearance of the main bearing in a tunneling machine. This work develops broadband PMUTs with a small blind area and high resolution for distance measurement in narrow and confined spaces, opening up a new path for ultrasonic ranging technology. Full article
(This article belongs to the Section Industrial Sensors)
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18 pages, 2421 KiB  
Article
Self-Adjusting Look-Ahead Distance of Precision Path Tracking for High-Clearance Sprayers in Field Navigation
by Xu Wang, Bo Zhang, Xintong Du, Huailin Chen, Tianwen Zhu and Chundu Wu
Agronomy 2025, 15(6), 1433; https://doi.org/10.3390/agronomy15061433 - 12 Jun 2025
Viewed by 611
Abstract
As a core component of agricultural machinery autonomous navigation, path tracking control holds significant research value. The pure pursuit algorithm has become a prevalent method for agricultural vehicle navigation due to its effectiveness at low speeds, yet its performance critically depends on the [...] Read more.
As a core component of agricultural machinery autonomous navigation, path tracking control holds significant research value. The pure pursuit algorithm has become a prevalent method for agricultural vehicle navigation due to its effectiveness at low speeds, yet its performance critically depends on the selection of the look-ahead distance. The conventional approaches require extensive parameter tuning due to the complex influencing factors, while fixed look-ahead distances struggle to balance the tracking accuracy and adaptability. Considerable effort is required to fine-tune the system to achieve optimal performance, which directly affects the accuracy of the path tracking and the results in the cumbersome task of selecting an appropriate goal point for the tracking path. To address these challenges, this paper introduces a pure pursuit algorithm for high-clearance sprayers in agricultural machinery, utilizing a self-adjusting look-ahead distance. By developing a kinematic model of the pure pursuit algorithm for agricultural machinery, an evaluation function is then employed to estimate the pose of the machinery and identify the corresponding optimal look-ahead distance within the designated area. This is done based on the principle of minimizing the overall error, enabling the dynamic and adaptive optimization of the look-ahead distance within the pure pursuit algorithm. Finally, this algorithm was verified in simulations and bumpy field tests under various different conditions, with the average value of the lateral error reduced by more than 0.06 m and the tuning steps also significantly reduced compared to the fixed look-ahead distance in field tests. The tracking accuracy has been improved and the applicability of the algorithm for rapid deployment has been enhanced. Full article
(This article belongs to the Special Issue Robotics and Automation in Farming)
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23 pages, 11820 KiB  
Article
High-Speed Nanoindentation and Local Residual Stress Analysis for Cut Edge Damage in Complex Phase Steels for Automotive Applications
by Laia Ortiz-Membrado, Sergi Parareda, Daniel Casellas, Emilio Jiménez-Piqué and Antonio Mateo
Metals 2025, 15(6), 651; https://doi.org/10.3390/met15060651 - 11 Jun 2025
Viewed by 1171
Abstract
Advanced high-strength steels (AHSSs) are used as lightweight solutions for vehicles, mainly focusing on the Body-in-White. However, the implementation of such steels for chassis parts requires a profound knowledge of the key design parameters for these components, particularly those concerning fatigue performance. Manufacturing [...] Read more.
Advanced high-strength steels (AHSSs) are used as lightweight solutions for vehicles, mainly focusing on the Body-in-White. However, the implementation of such steels for chassis parts requires a profound knowledge of the key design parameters for these components, particularly those concerning fatigue performance. Manufacturing of chassis parts include mechanical cutting operations. Therefore, the deformation and damage induced at the cut edge may affect the fatigue resistance of the parts in service. To characterize and study this critical area, damage and micromechanical properties have been evaluated at the cut edge for three different AHSS grades, CP800, CP980, and DP600, analyzing the impact of cutting parameters and post-processing treatments, such as sandblasting. Large high-speed nanoindentation maps of 400 × 200 µm2 have been carried out along the cut edge in the three different target zones: burnish, fracture, and burr. In the hardness maps, the deformation lines and the gradient of hardness with increasing distance from the cut edge are perfectly observed. Residual stresses at the target zones of the cut edges were measured using the FIB-DIC method for CP980 to complement the micromechanical study in these critical areas. The results found show that reduced cutting clearance leads to larger hardened zones and favorable compressive stress distributions, correlating with improved fatigue resistance. Hardened zones extending up to 100 µm from the cut edge and compressive residual stresses exceeding −300 MPa were observed at low clearance. These findings are consistent with numerical simulations and previous fatigue tests, highlighting the potential of combining high-speed nanoindentation and local stress analysis for optimizing shear cutting processes in AHSS components. Full article
(This article belongs to the Special Issue Microstructure and Mechanical Behavior of High-Strength Steel)
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27 pages, 9007 KiB  
Article
Middle Rock Pillar Stability Criteria for a Bifurcated Small Clear-Distance Tunnel
by Jianxiu Wang, Yanxia Long, Ansheng Cao, Tao Cui, Luyu Lin, Yuanbo Gao, Xuezeng Liu and Huboqiang Li
Appl. Sci. 2025, 15(10), 5634; https://doi.org/10.3390/app15105634 - 18 May 2025
Viewed by 422
Abstract
Middle rock pillars (MRPs) play a crucial role in the stability of bifurcated small clear-distance tunnels. Assessing the stability of the MRP is a key challenge in design and construction. This study focuses on the bifurcated small clear-distance section of the Xiamen Haicang [...] Read more.
Middle rock pillars (MRPs) play a crucial role in the stability of bifurcated small clear-distance tunnels. Assessing the stability of the MRP is a key challenge in design and construction. This study focuses on the bifurcated small clear-distance section of the Xiamen Haicang Shugang evacuation channel underground interchange tunnels. The stability criteria for the MRP during both the early design and later construction stages were analyzed by using the strength reduction method (SRM) via numerical simulations. In the design stage, the SRM was applied to determine the stability limit state of the MRP. Relationships between rock mass density, cohesion, and elastic modulus were identified, and these parameters were combined with basic cohesion values for an initial stability assessment. During the construction stage, the full excavation process was analyzed by examining the distribution and changes in the plastic zone of the rock mass. Two key construction stages, a 10 m excavation on the main line upper step and a 10 m excavation on the ramp upper step, were identified as points where the plastic zone of the MRP began to form on the sidewall and the center, respectively. Multiple linear regression was used to determine the displacement, stress, and plasticity criteria for MRP stability. A comprehensive criteria formula incorporating the width–span ratio, tunnel vault settlement, and horizontal clearance convergence was developed, providing technical guidance and a scientific basis for similar projects. Full article
(This article belongs to the Section Civil Engineering)
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22 pages, 30414 KiB  
Article
Metric Scaling and Extrinsic Calibration of Monocular Neural Network-Derived 3D Point Clouds in Railway Applications
by Daniel Thomanek and Clemens Gühmann
Appl. Sci. 2025, 15(10), 5361; https://doi.org/10.3390/app15105361 - 11 May 2025
Viewed by 564
Abstract
Three-dimensional reconstruction using monocular camera images is a well-established research topic. While multi-image approaches like Structure from Motion produce sparse point clouds, single-image depth estimation via machine learning promises denser results. However, many models estimate relative depth, and even those providing metric depth [...] Read more.
Three-dimensional reconstruction using monocular camera images is a well-established research topic. While multi-image approaches like Structure from Motion produce sparse point clouds, single-image depth estimation via machine learning promises denser results. However, many models estimate relative depth, and even those providing metric depth often struggle with unseen data due to unfamiliar camera parameters or domain-specific challenges. Accurate metric 3D reconstruction is critical for railway applications, such as ensuring structural gauge clearance from vegetation to meet legal requirements. We propose a novel method to scale 3D point clouds using the track gauge, which typically only varies in very limited values between large areas or countries worldwide (e.g., 1.435 m in Europe). Our approach leverages state-of-the-art image segmentation to detect rails and measure the track gauge from a train driver’s perspective. Additionally, we extend our method to estimate a reasonable railway-specific extrinsic camera calibration. Evaluations show that our method reduces the average Chamfer distance to LiDAR point clouds from 1.94 m (benchmark UniDepth) to 0.41 m for image-wise calibration and 0.71 m for average calibration. Full article
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20 pages, 7275 KiB  
Article
Deformation Patterns and Control of Existing Tunnels Induced by Coastal Foundation Pit Excavation
by Tao Liu, Yunlong Liang, Huadong Peng, Liucheng Yu, Tongju Xing, Yuanzhe Zhan and Jianguo Zheng
J. Mar. Sci. Eng. 2025, 13(4), 773; https://doi.org/10.3390/jmse13040773 - 13 Apr 2025
Viewed by 432
Abstract
The rapid development of coastal cities has intensified land resource constraints and is leading to an increasing number of foundation pit projects near existing operational tunnels. This necessitates careful consideration of coastal excavation impacts on adjacent tunnels. Taking a foundation pit project in [...] Read more.
The rapid development of coastal cities has intensified land resource constraints and is leading to an increasing number of foundation pit projects near existing operational tunnels. This necessitates careful consideration of coastal excavation impacts on adjacent tunnels. Taking a foundation pit project in Qingdao as a case study, this paper investigates tunnel deformation through statistical analysis, numerical simulation, and field monitoring. By adjusting numerical model parameters, the research examines the influence of horizontal clearance distances, existing structure burial depths, and different retaining structure configurations on tunnel deformation, providing guidance for deformation control. Key findings include the following: (1) Statistical analysis reveals that tunnels in silty clay strata experience more significant excavation-induced deformation compared to those in silt strata, with relative positional relationships between pits and tunnels playing a critical role. (2) Numerical and monitoring results demonstrate that pit excavation induces tunnel displacement towards the excavation zone. Maximum lateral displacement reached 3.57 mm (simulated) and 4.79 mm (measured), while maximum vertical displacement was 3.11 mm (simulated) and 3.85 mm (measured), all within safety thresholds. (3) Sensitivity analysis shows that shallower tunnels exhibit more pronounced deformations. Increasing horizontal separation distance from 10 m to 25 m reduces deformation by one-third. However, adjusting diaphragm wall thickness and retaining structure embedment depth proves limited in deformation control, necessitating reinforcement measures on the tunnel side. These findings provide valuable references for protecting coastal silty clay stratum tunnels. Full article
(This article belongs to the Special Issue Advances in Marine Geological and Geotechnical Hazards)
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22 pages, 7429 KiB  
Article
Nonlinear Dynamic Modeling of a Gear-Bearing Transmission System Based on Dynamic Meshing Parameters
by Jinzhou Song, Lei Hou, Rui Ma, Zhonggang Li, Rongzhou Lin, Yi Chen, Yushu Chen and Nasser A. Saeed
Machines 2025, 13(3), 230; https://doi.org/10.3390/machines13030230 - 12 Mar 2025
Cited by 2 | Viewed by 671
Abstract
The nonlinear contact force between gears and bearings exhibits intricate dynamics. This paper focuses on the coupling relationship between the time-varying meshing parameters of the gears, dynamic backlash, and dynamic bearing clearance in gear-bearing transmission systems. A dynamic model of a gear-bearing transmission [...] Read more.
The nonlinear contact force between gears and bearings exhibits intricate dynamics. This paper focuses on the coupling relationship between the time-varying meshing parameters of the gears, dynamic backlash, and dynamic bearing clearance in gear-bearing transmission systems. A dynamic model of a gear-bearing transmission system considering dynamic meshing parameters is established. The coupling mechanism between meshing stiffness, gear backlash, bearing clearance, and gear vibration response in gear transmission systems is analyzed. The results demonstrate a negative correlation between the gears’ geometric center distance and meshing stiffness amplitude. Gear vibration can affect the relative position of the gears. Changes in the relative position of the gears lead to an increase in the number of frequency components in the frequency domain of gear meshing stiffness. During gear rotation, the meshing parameters of the gears and tooth side clearance fluctuate with gear vibration. With increasing speed, the model’s dynamic meshing parameters also increase accordingly. The model achieves a feedback calculation of the system parameters and vibration responses in gear-bearing system dynamics. Full article
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15 pages, 3394 KiB  
Article
Experimental Research on and Optimization of Plasma Emitter Sources
by Xu Gao, Jing Zhou and Xiao Du
Sensors 2025, 25(6), 1715; https://doi.org/10.3390/s25061715 - 10 Mar 2025
Viewed by 601
Abstract
Traditional emitters used for downhole acoustic detection have limited radiation frequency and energy, making it difficult to transmit high-precision acoustic signals over long distances. This paper presents a plasma emitter in which high-pressure discharge generates a powerful spherical impulse wave with a wide [...] Read more.
Traditional emitters used for downhole acoustic detection have limited radiation frequency and energy, making it difficult to transmit high-precision acoustic signals over long distances. This paper presents a plasma emitter in which high-pressure discharge generates a powerful spherical impulse wave with a wide frequency range. First, the discharge characteristics of the plasma needle-plate emitter are analyzed using high-voltage discharge experiments and discharge simulation models for underwater emitters. Subsequently, advanced modifications are made to the structure of the needle–plate emitter to meet the requirements of downhole detection. A new type of hollow needle–plate emitter with a spherical tip is developed. The results show that the structural optimization of the hollow needle–plate emitter with a spherical tip resulted in a 27.2% increase in impulse wave amplitude, a 28.1% improvement in electro-acoustic conversion efficiency, and a radiation frequency band covering up to 100 kHz. This development is conducive to more accurate and longer-range downhole structure detection. The detection range outside the borehole can reach tens to hundreds of meters. This enables the precise control of the wellbore path and reduces the demands on the rig’s build rate. The emitter has significant application potential in areas such as onshore and offshore oil and gas exploration, unconventional resource detection, impulse wave fracturing and wellbore clearance, and rescue and U-well drilling. Full article
(This article belongs to the Section Electronic Sensors)
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11 pages, 1718 KiB  
Article
Obstacle Circumvention Strategies During Omnidirectional Treadmill Walking in Virtual Reality
by Marco A. Bühler and Anouk Lamontagne
Sensors 2025, 25(6), 1667; https://doi.org/10.3390/s25061667 - 8 Mar 2025
Viewed by 867
Abstract
Obstacle circumvention is an important task for community ambulation that is challenging to replicate in research and clinical environments. Omnidirectional treadmills combined with virtual reality (ODT-VR) offer a promising solution, allowing users to change walking direction and speed while walking in large, simulated [...] Read more.
Obstacle circumvention is an important task for community ambulation that is challenging to replicate in research and clinical environments. Omnidirectional treadmills combined with virtual reality (ODT-VR) offer a promising solution, allowing users to change walking direction and speed while walking in large, simulated environments. However, the extent to which such a setup yields circumvention strategies representative of overground walking in the real world (OVG-RW) remains to be determined. This study examined obstacle circumvention strategies in ODT-VR versus OVG-RW and measured how they changed with practice. Fifteen healthy young individuals walked while avoiding an interferer, performing four consecutive blocks of trials per condition. Distance at onset trajectory deviation, minimum distance from the interferer, and walking speed were compared across conditions and blocks. In ODT-VR, larger clearances and slower walking speeds were observed. In contrast, onset distances and proportions of right-side circumvention were similar between conditions. Walking speed increased from the first to the second block exclusively. Results suggest the use of a cautious locomotor behavior while using the ODT-VR setup, with some key features of circumvention strategies being preserved. Although ODT-VR setups offer exciting prospects for research and clinical applications, consideration should be given to the generalizability of findings to the real world. Full article
(This article belongs to the Special Issue Advanced Sensors in Biomechanics and Rehabilitation)
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25 pages, 7806 KiB  
Article
A Simple Single-Ended Post-Fault Location Technique for DC Lines Based on Controlled Re-Energizations
by Kumar Mahtani, José M. Guerrero, Julien Decroix and Carlos A. Platero
Electronics 2025, 14(2), 275; https://doi.org/10.3390/electronics14020275 - 11 Jan 2025
Cited by 2 | Viewed by 843
Abstract
Fault location in medium-voltage direct current (MVDC) systems is an essential yet underexplored area compared to high-voltage (HVDC) and low-voltage (LVDC) systems. MVDC systems, characterized by intermediate line lengths and fault resistances, as well as rapid fault clearance requirements, demand specialized solutions. This [...] Read more.
Fault location in medium-voltage direct current (MVDC) systems is an essential yet underexplored area compared to high-voltage (HVDC) and low-voltage (LVDC) systems. MVDC systems, characterized by intermediate line lengths and fault resistances, as well as rapid fault clearance requirements, demand specialized solutions. This paper proposes a novel single-ended, offline fault location method based on controlled re-energizations after fault clearance. This approach employs a switched grounding resistor and a bypass connection through the current-limiting inductor to extract fault parameters from the discharge curves of two re-energization cycles. By analyzing the time constants derived from these curves, the method estimates fault location and resistance with high accuracy. The proposed method eliminates the need for additional active injection sources and circuit breaker modifications, ensuring seamless integration into existing MVDC infrastructure. Furthermore, the method avoids inter-terminal communication delays and sampling delays before fault clearance. Validation through electromagnetic transient simulations demonstrates fault location errors below 5% for fault resistances up to 50 Ω. Results show that the method performs better for faults farther from the active terminal, with the higher errors seen for short distances and elevated resistances. The proposed technique offers a robust and practical solution for post-fault location in DC lines. Full article
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16 pages, 6250 KiB  
Article
Automatic Control System for Maize Threshing Concave Clearance Based on Entrainment Loss Monitoring
by Yang Yu, Yi Cheng, Chenlong Fan, Liyuan Chen, Qinhao Wu, Mengmeng Qiao and Xin Zhou
Processes 2025, 13(1), 58; https://doi.org/10.3390/pr13010058 - 30 Dec 2024
Cited by 2 | Viewed by 1071
Abstract
Complex harvesting environments and varying crop conditions often lead to threshing cylinder blockage and increased entrainment loss in maize grain harvesters. To address these issues, an electric-driven automatic control system for maize threshing concave clearance based on real-time entrainment loss monitoring was developed. [...] Read more.
Complex harvesting environments and varying crop conditions often lead to threshing cylinder blockage and increased entrainment loss in maize grain harvesters. To address these issues, an electric-driven automatic control system for maize threshing concave clearance based on real-time entrainment loss monitoring was developed. The system automatically adjusts concave clearance parameters at different harvesting speeds to maintain grain entrainment loss within an optimal range. First, an adjustable concave structure based on a crank-link mechanism was designed, with a threshing clearance adjustment range of 15–47 mm and motor rotation angle of 0–48°. Subsequently, an EDEM simulation model of the mixed material discharge inside the threshing cylinder was established to determine the optimal installation position of the entrainment loss monitoring sensor based on piezoelectric ceramic-sensitive elements. The sensor was positioned at the left tail end of the concave sieve, with a minimum distance of 58 mm between the sensitive plate centerline and threshing concave sieve and an installation angle of 65° relative to the horizontal plane. A maize threshing clearance control method based on fuzzy neural network PID control algorithm was proposed, and Simulink simulation optimization verified its superior performance with fast response speed. After system integration, field trials were conducted at low, medium, and high operating speeds with preset ideal entrainment loss intervals. The results showed that control was unnecessary at low speed, the control system-maintained entrainment loss within set range at medium speed, and maximum threshing clearance was needed at high speed. Finally, comparative trials of threshing performance with and without the control system were conducted at medium harvesting speed. Results showed that the entrainment loss rate decreased by 43.75% with the control system activated, significantly reducing maize threshing entrainment losses. This study overcame the barrier of maize threshing parameter adjustment being heavily reliant on manual experience and provided theoretical support for the intelligent grain harvesting equipment. Full article
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30 pages, 5433 KiB  
Review
Iron Oxide Nanoparticle-Based T1 Contrast Agents for Magnetic Resonance Imaging: A Review
by Dongmei Zhang, Jing Zhang, Xianglin Bian, Pei Zhang, Weihua Wu and Xudong Zuo
Nanomaterials 2025, 15(1), 33; https://doi.org/10.3390/nano15010033 - 28 Dec 2024
Cited by 4 | Viewed by 2458
Abstract
This review highlights recent progress in utilizing iron oxide nanoparticles (IONPs) as a safer alternative to gadolinium-based contrast agents (GBCAs) for magnetic resonance imaging (MRI). It consolidates findings from multiple studies, discussing current T1 contrast agents (CAs), the synthesis techniques for IONPs, [...] Read more.
This review highlights recent progress in utilizing iron oxide nanoparticles (IONPs) as a safer alternative to gadolinium-based contrast agents (GBCAs) for magnetic resonance imaging (MRI). It consolidates findings from multiple studies, discussing current T1 contrast agents (CAs), the synthesis techniques for IONPs, the theoretical principles for designing IONP-based MRI CAs, and the key factors that impact their T1 contrast efficacy, such as nanoparticle size, morphology, surface modifications, valence states, and oxygen vacancies. Furthermore, we summarize current strategies to achieve IONP-based responsive CAs, including self-assembly/disassembly and distance adjustment. This review also evaluates the biocompatibility, organ accumulation, and clearance pathways of IONPs for clinical applications. Finally, the challenges associated with the clinical translation of IONP-based T1 CAs are included. Full article
(This article belongs to the Section Biology and Medicines)
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20 pages, 20405 KiB  
Article
The Role of Bone Marrow Stromal Cell Antigen 2 (BST2) in the Migration of Dendritic Cells to Lymph Nodes
by Sehoon Park, Eunbi Yi, Jaemyeong Jeon, Jinsoo Oh, Zhengmei Xu and Se-Ho Park
Int. J. Mol. Sci. 2025, 26(1), 149; https://doi.org/10.3390/ijms26010149 - 27 Dec 2024
Viewed by 1243
Abstract
Bone marrow stromal antigen 2 (BST2) is a host-restriction factor that plays multiple roles in the antiviral defense of innate immune responses, including the inhibition of viral particle release from virus-infected cells. BST2 may also be involved in the endothelial adhesion and migration [...] Read more.
Bone marrow stromal antigen 2 (BST2) is a host-restriction factor that plays multiple roles in the antiviral defense of innate immune responses, including the inhibition of viral particle release from virus-infected cells. BST2 may also be involved in the endothelial adhesion and migration of monocytes, but its importance in the immune system is still unclear. Immune cell adhesion and migration are closely related to the initiation of immune responses. In this study, we found that the expressions of the lymph node homing marker chemokine receptor 7 (CCR7) and an adhesion molecule intercellular adhesion molecule 1 (ICAM-1) in conventional dendritic cells (cDCs) were associated with BST2 expression. Interestingly, Bst2−/− cDCs showed lower chemotactic ability, including velocity and accumulative distance toward chemokine ligand 19 (CCL19) gradient in vitro, compared to wild-type cDCs. Bst2−/− cDCs also showed reduced migration and reduced retention capacity in draining lymph nodes in vivo. As a result, Bst2−/− cDCs as antigen-presenting cells induced lower antigen-specific B cell and T cell responses compared to Bst2+/+ cDCs. Notably, mice administered the influenza vaccine via Bst2−/− cDCs exhibited substantially inefficient virus clearance compared to mice administered the Bst2+/+ cDCs vaccine. Therefore, we propose that BST2, which plays a critical role in the effective migration and retention of cDCs, is involved in the development of optimal immunological effects in draining lymph nodes. Full article
(This article belongs to the Section Molecular Immunology)
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13 pages, 860 KiB  
Article
Impact of Overweight on Spatial–Temporal Gait Parameters During Obstacle Crossing in Young Adults: A Cross-Sectional Study
by Matthias Chardon, Fabio Augusto Barbieri, Clint Hansen, Pascal Petit and Nicolas Vuillerme
Sensors 2024, 24(23), 7867; https://doi.org/10.3390/s24237867 - 9 Dec 2024
Viewed by 1139
Abstract
Background: Overweight may present an additional challenge when crossing obstacles. More specifically it may affect adequate foot clearance to reduce the risk of obstacle contact. Thus, the objective of this study was to compare obstacle clearance and spatial–temporal gait parameters during obstacle [...] Read more.
Background: Overweight may present an additional challenge when crossing obstacles. More specifically it may affect adequate foot clearance to reduce the risk of obstacle contact. Thus, the objective of this study was to compare obstacle clearance and spatial–temporal gait parameters during obstacle crossing in young adults with normal body weight and overweight. Methods: Twenty-eight and fifteen individuals were categorized into normal body mass index (18.5–25 kg/m2) and overweight (25–30 kg/m2), respectively. The participants walked along a walkway at their preferred speed and stepped over an obstacle. Spatial–temporal parameters were calculated during the approaching (stride before obstacle) and the crossing (step over the obstacle) phases. Additionally, the leading and trailing foot placements prior to and after the obstacle and toe clearance were calculated. Results: No significant differences were found for the approach, the crossing phases and leading and trailing toe clearance. Analysis of foot placement distance prior to and after the obstacle showed that, compared to the individuals with normal body weight, overweight individuals significantly increased the leading foot placement distance prior to the obstacle (+7 cm, ↑ 6.7%) and increased the trailing foot placement distance after the obstacle (+8.1 cm, ↑ 9%). Conclusions: Our findings indicated that overweight individuals have a different obstacle crossing behavior regarding foot placement distance prior to and after the obstacle compared to normal-weight individuals without differences in spatial–temporal gait parameters or toe clearances. However, the results did not suggest that participants with overweight show a higher risk of tripping. Full article
(This article belongs to the Section Intelligent Sensors)
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