Search Results (21,253)

Control valves in nuclear systems operate under high-pressure differentials generating intense transient fluid forces that induce destructive structural vibrations, risking resonance and the valve stem fracture. In this study, computational fluid dynamics (CFD) was employed to characterize the internal flow dynamics of the valve, supported by experiment validation of the fluid model. To account for nonlinear structural effects such as contact and damping, a coupled fluid–structure interaction approach incorporating nonlinear perturbation analysis was applied to evaluate the dynamic response of the valve core assembly under fluid excitation. The results indicate that flow separation, re-circulation, and vortex shedding within the throttling region are primary contributors to structural vibrations. A comparative analysis of stability coefficients, modal damping ratios, and logarithmic decrements under different valve openings revealed that the valve core assembly remains relatively stable overall. However, critical stability risks were identified in the lower-order modal frequency range at 50% and 70% openings. Notably, at a 70% opening, the first-order modal frequency of the valve core assembly closely aligns with the frequency of fluid excitation, indicating a potential for critical resonance. This research provides important insights for evaluating and enhancing the vibration stability and operational safety of control valves under complex flow conditions. Full article

Background/Objectives: Gut-derived tryptophan (Trp) metabolites play important roles in metabolic and cardiovascular regulation. Although animal studies suggest their protective effects against metabolic dysfunction, data in adolescents, particularly those with obesity, remain limited. The objective of this study was to evaluate associations between circulating gut-derived Trp metabolites and markers of cardiometabolic, vascular, and platelet health in adolescents with obesity. Methods: Data were analyzed from 28 adolescents (ages 13–18; mean BMI = 36 ± 6.4 kg/m²). Fasting blood was collected to assess lipid profiles using a clinical analyzer and insulin resistance using the homeostatic model assessment for insulin resistance (HOMA-IR). Gut-derived Trp metabolites were measured by UPLC–mass spectrometry, peak oxygen uptake (VO_{2 peak}) by gas exchange during an incremental cycle ergometer test, and body composition by dual-energy X-ray absorptiometry. Platelet spare respiratory capacity (SRC), endothelial function, and liver fat were measured using high-resolution respirometry, flow-mediated dilation (FMD) of the brachial artery, and magnetic resonance imaging respectively. Results: Indole-3-propionic acid was inversely associated with diastolic blood pressure (rho = −0.39, p = 0.047), total cholesterol (rho = −0.55, p = 0.002), and LDL-C (rho = −0.57, p = 0.0014), independent of sex and obesity severity. Indoxyl sulfate was positively correlated with fasting glucose (rho = 0.47, p = 0.012), and adolescents with impaired fasting glucose had 1.6-fold higher IS levels. Indole-3-acetaldehyde declined with age (rho = −0.50, p = 0.007), and Indole-3-acetic acid and indole were higher in Hispanics vs. non-Hispanics. No significant associations were observed between Trp metabolites and FMD, VO₂ peak, or SRC. Conclusions: Gut-derived Trp metabolites, particularly indole-3-propionic and indoxyl sulfate, are associated with markers of cardiometabolic risk in adolescents with obesity. These findings support their potential relevance in early-onset cardiovascular disease risk. Full article

Co-infections of Orthopoxviruses (OPVs), such as vaccinia virus (VACV) and monkeypox virus (MPXV), and the human immunodeficiency virus (HIV) can be associated with severe outcomes. Serro’s dairy region, located in Minas Gerais, southeastern Brazil, is an endemic area for VACV, where zoonotic outbreaks affect rural communities. This epidemiological context is especially relevant for at-risk populations, such as people living with HIV (PLHIV). This study aimed to assess the presence of neutralizing antibodies (NAbs) against OPV in PLHIV in this endemic setting. Serum samples were collected from 177 PLHIV in treatment at the specialized service between December 2021 and August 2022. VACV and MPXV NAbs were measured using the plaque reduction neutralization test (PRNT) and VACV-infected cells. The overall occurrence of OPV NAbs was 27.7%. NAbs were higher in individuals born before 1980 (53.3%) than those born after 1980 (1.1%). Among anti-VACV-seropositive individuals, 40.8% also had MPXV NAbs, suggesting cross-immunity. These findings indicate the circulation of VACV in PLHIV and highlight the increased susceptibility to OPV infections among individuals born after the cessation of smallpox vaccination. The results reinforce the importance of continued surveillance of OPV, especially in endemic regions and vulnerable populations. Full article

Respiratory pathogens are significant causes of morbidity and mortality worldwide. Since the emergence of SARS-CoV-2 in 2019 and the mitigation measures implemented to control the pandemic, other respiratory viruses’ transmission and circulation patterns were substantially disrupted. We leveraged the influenza hospitalization surveillance in Tanzania to understand the distribution of respiratory viruses shortly after nonpharmaceutical interventions (NPIs) were lifted. A total of 475 samples that tested negative for SARS-CoV-2 and influenza from March through May 2022 were included in this study. The samples were tested for 16 virus targets using Anyplex II RV16 multiplex assays. The findings indicate that most hospitalizations (74%) were among children under 15 years, with human bocavirus (HBoV) being the most prevalent (26.8%), followed by rhinovirus (RV, 12.3%), parainfluenza viruses (PIVs1–4, 10.2%), respiratory syncytial virus (RSV, 8.7%), adenovirus (AdV, 4.3%), and metapneumovirus (MPV, 2.9%). Notably, 54% of respiratory hospitalizations had no viruses detected. The findings highlight the broad circulation of respiratory viruses shortly after NPIs were lifted in Tanzania. Surveillance for respiratory pathogens beyond influenza and SARS-CoV-2 can inform public health officials of emerging threats in the country and should be considered an important pandemic preparedness measure at a global level. Full article

Limited molecular data exist on zoonotic parasites Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Angus calves from Guizhou, China. This study constitutes the first molecular epidemiological survey of these pathogens in this region. 817 fecal samples from Angus calves across 7 intensive beef farms (Bijie City). Nested PCR methods targeting SSU rRNA (Cryptosporidium spp.), gp60 (Cryptosporidium bovis subtyping), bg/gdh/tpi (G. duodenalis), and ITS (E. bieneusi) coupled with DNA sequencing were employed. DNA sequences were analyzed against the NCBI. database. Statistical differences were assessed via a generalized linear mixed-effects model. Cryptosporidium spp. prevalence 23.5% (192/817; 95% CI 28.1–34.6%), with C. bovis predominating 89.6% (172/192; 95% CI 84.4–93.5%) and six subtypes (XXVIa-XXVIf). Highest infection in 4–8-week-olds 29.9% (143/479; 95% CI 25.8–34.1%) (p < 0.01). G. duodenalis: 31.3% (256/817; 95% CI 28.1–34.6%) positive, overwhelmingly assemblage E 97.6% (6/256; 95% CI 0.9–5.0%), zoonotic assemblage A was marginal 0.7% (6/817; 95% CI 0.3–1.6%). Farm-level variation exceeded 10-fold (e.g., Gantang: 55.0% (55/100; 95% CI 44.7–65.0%) vs. Tieshi: 4.9% (5/102; 95% CI 1.6–11.1%). E. bieneusi: prevalence 19.7% (161/817; 95% CI 17.0–22.6%), exclusively zoonotic genotypes BEB4: 49.7% (80/161; 95% CI 41.7–57.7%); I: 40.4% (65/161; 95% CI 32.7–48.4%). Strong diarrhea association (p < 0.01) and site-specific patterns (e.g., Guanyindong: 39.2%). While Giardia exhibited the highest prevalence (31.3%) with minimal zoonotic risk, Enterocytozoon—despite lower prevalence (19.7%)—posed the greatest public health threat due to exclusive circulation of human-pathogenic genotypes (BEB4/I) and significant diarrhea association, highlighting divergent control priorities for these enteric parasites in Guizhou calves. Management/Public health impact: Dominant zoonotic E. bieneusi genotypes (BEB4/I) necessitate: 1. Targeted treatment of 4–8-week-old Angus calves. 2. Manure biofermentation (≥55 °C, 3 days), and 3. UV-disinfection (≥1 mJ/cm²) for karst water to disrupt transmission in this high-humidity region. Full article

The COVID-19 pandemic significantly impacted the circulation, seasonality, and disease burden of viral respiratory infections. This study aimed to evaluate the impact of SARS-CoV-2 on the frequency of viral respiratory infections at a teaching hospital in Southern Italy by comparing data from before, during, and after the COVID-19 pandemic and by investigating how the emergence of SARS-CoV-2 affected the circulation and seasonality of other respiratory viruses. This retrospective and prospective study was performed on de-identified nasopharyngeal specimens classified as pre-COVID-19 (before 15 March 2020), during-COVID-19 (from 16 March 2020 to 5 May 2023), and post-COVID-19 (from 6 May 2023 to 31 December 2024). Overall, 790 out of 3930 (20%) patient samples tested positive for at least one respiratory virus. The mean age of patients was 60 ± 19 years, with significant positivity rates observed in the 65–98 age group (p ≤ 0.05) across all periods. In the pre-COVID-19 period, the most prevalent virus was influenza A (47.5%, 47/99), followed by the human rhinovirus (19.2%, 19/99). During the COVID-19 pandemic, SARS-CoV-2 was the most prevalent (64.9%, 290/447), before decreasing to 38% (92/244) after the pandemic, while influenza A’s positivity prevalence increased to 14.3% (35/244). Rhinovirus/enterovirus remained relatively stable throughout all periods. The pandemic notably altered viral co-infection dynamics, with its effects lasting into the post-COVID-19 period. Specifically, a marked decrease in influenza A circulation was observed, while respiratory syncytial virus (RSV) epidemiology remained stable and significant co-circulation of rhinovirus/enterovirus with SARS-CoV-2 persisted. Therefore, since COVID-19 and influenza affect the same high-risk groups, those individuals must be vaccinated against both viruses. Full article

Bacterial meningitis remains a critical public health issue globally due to its high morbidity and mortality. Understanding regional epidemiological trends is essential to inform vaccination strategies and public health interventions. This observational, retrospective study analyzed cerebrospinal fluid (CSF) isolates collected from 731 confirmed cases of bacterial meningitis between 2014 and 2024 in Lombardy, Italy. Pathogen identification and serotyping of Streptococcus pneumoniae (SP), Neisseria meningitidis (NM), and Haemophilus influenzae (HI) were conducted using culture-based and molecular techniques. Trends were assessed across age groups and time using Kruskal–Wallis and chi-square tests. Results: SP was the predominant pathogen (78.4%), followed by NM (13.0%) and HI (8.6%). Significant temporal variation was observed for SP and NM, while HI trends remained stable. The impact of COVID-19-related restrictions was evident in a reduction in cases during 2020–2021. SP serotypes 3 and 8, HI non-typeable strains, and NM serogroup B were most frequent. No major shifts in serotype distribution were observed. Long-term surveillance data from Lombardy underscore the dominance of vaccine-targeted serotypes, ongoing circulation of resilient clones, and post-pandemic epidemiological shifts. These findings support continuous surveillance and inform vaccine strategy adjustments at the regional and national levels. Full article

Nokoué Lake is located in the south of Benin and is fed by the Ouémé and Sô Rivers. Its hydrosedimentary dynamics were modelled using Telemac2D, incorporating the main environmental factors of this complex ecosystem. The simulations accounted for flow rates and suspended solids concentrations during periods of high and low water. The main factors controlling sediment transport were identified. The model was validated using field measurements of water levels and suspended solids. The results show that the north–south current velocity ranges from 0.5 to 1 m/s during periods of high water and 0.1 to 0.5 m/s during low-water periods. Residual currents are influenced by rainfall, river discharge, and tides. Complex circulation patterns are caused by increased river flow during high water, while tides dominate during low water and transitional periods. The northern, western, and south-eastern parts of the lake have weak residual currents and are, therefore, deposition zones for fine sediments. The estimated average annual suspended solids load for 2022–2023 is 17 Mt. The model performance shows a strong agreement between the observed and simulated values: R² = 0.91 and NSE = 0.93 for water levels and R² = 0.86 and NSE = 0.78 for sediment transport. Full article

Thyroid hormones (thyroxine, T₄, and triiodothyronine, T₃) are indispensable for sustaining vertebrate life, and their deficiency gives rise to a wide range of symptoms characteristic of hypothyroidism, affecting 5–10% of the world’s population. The precursor for thyroid hormone synthesis is thyroglobulin (Tg), a large iodoglycoprotein consisting of upstream regions I-II-III (responsible for synthesis of most T₄) and the C-terminal CholinEsterase-Like (ChEL) domain (responsible for synthesis of most T₃, which can also be generated extrathyroidally by T₄ deiodination). Using CRISPR/Cas9-mediated mutagenesis, we engineered a knock-in of secretory ChEL into the endogenous TG locus. Secretory ChEL acquires Golgi-type glycans and is properly delivered to the thyroid follicle lumen, where T₃ is first formed. Homozygous knock-in mice are capable of thyroidal T₃ synthesis but largely incompetent for T₄ synthesis such that T₄-to-T₃ conversion contributes little. Instead, T₃ production is regulated thyroidally by thyrotropin (TSH). Compared to cog/cog mice with conventional hypothyroidism (low serum T₄ and T₃), the body size of ChEL-knock-in mice is larger; although, these animals with profound T₄ deficiency did exhibit a marked elevation of serum TSH and a large goiter, despite normal circulating T₃ levels. ChEL knock-in mice exhibited a normal expression of hepatic markers of thyroid hormone action but impaired locomotor activities and increased anxiety-like behavior, highlighting tissue-specific differences in T₃ versus T₄ action, reflecting key considerations in patients receiving thyroid hormone replacement therapy. Full article

Background: Intravascular albumin mass represents the total quantity of albumin circulating within the bloodstream and may serve as a physiologically relevant marker of vascular integrity and fluid distribution in sepsis. While low serum albumin levels are acknowledged as prognostic indicators, dynamic assessments based on albumin mass remain insufficiently explored in patients outside the intensive care unit. Objectives: To describe the temporal changes in intravascular albumin mass in patients with community-acquired sepsis and to examine its relationship with fluid balance and thirty-day mortality. Methods: This prospective observational study encompassed 247 adults diagnosed with community-acquired sepsis who were admitted to a high-dependency hospital ward specializing in acute medical care. The intravascular albumin mass was calculated daily for a duration of up to five days, utilizing plasma albumin concentration and estimated plasma volume derived from anthropometric and hematologic data. Net albumin leakage was defined as the variation in intravascular albumin mass between consecutive days. Fluid administration and urine output were documented to ascertain cumulative fluid balance. Repeated-measures statistical models were employed to evaluate the associations between intravascular albumin mass, fluid balance, and mortality, with adjustments made for age, comorbidity, and clinical severity scores. Results: The intravascular albumin mass exhibited a significant decrease during the initial five days of hospitalization and demonstrated an inverse correlation with the cumulative fluid balance. A greater net leakage of albumin was associated with a positive fluid balance and elevated mortality rates. Furthermore, a reduced intravascular albumin mass independently predicted an increased risk of mortality at thirty days. Conclusions: A reduction in intravascular albumin mass may suggest ineffective fluid retention and the onset of capillary leak syndrome. This parameter holds promise as a clinically valuable, non-invasive indicator for guiding fluid resuscitation in cases of sepsis. Full article

A novel modified oyster shell (MOS-800) was developed to enhance phosphorus sequestration and recovery from wastewater. Approximately 33.3% of phosphate was eliminated by the MOS-800, which also exhibited excellent pH regulation capabilities. In semicontinuous tests, a synergistic phosphorus separation was achieved through the coupling process of CaCl₂/MOS-800 and a circulating fluidized bed (CFB), resulting in an 86.5% phosphate separation. In continuous flow experiments, phosphorus elimination reached 98.2%. Material characterization revealed that hydroxyapatite (HAP) was the primary component of the crystallized products. Additionally, MOS-800 released 506.5–572.2 mg/g Ca²⁺ and 98.1 mg/g OH⁻. A four-stage heterogeneous crystallization mechanism was proposed for the coupling process. In the first stage, Ca²⁺ quickly reacted with phosphate to form Ca-P ion clusters, etc. In the second stage, these clusters packed randomly to form spherical amorphous calcium phosphate (ACP). In the third stage, the ACP spheres were transformed and rearranged into sheet-like HAP crystallites, Finally, in the fourth stage, the HAP crystallites aggregated on the surface of crystal seeds, also with the addition of crystal seeds and undissolved MOS-800, potentially catalyzing the heterogeneous crystallization. These findings suggest that the CaCl₂/MOS-800/CFB system is a promising technique for phosphate recovery from wastewater. Full article

There are currently no approved therapeutic treatments targeting metabolic dysfunction-associated steatotic liver disease (MASLD). Albumin, a liver-produced plasma protein with anti-inflammatory and antioxidant properties, is reduced in advanced liver disease. Considering the role of chronic obesity-induced inflammation in MASLD pathogenesis, we investigated whether albumin administration could prevent disease progression to metabolic dysfunction-associated steatohepatitis (MASH). MASLD was induced in mice using a high-fat and high-cholesterol (PC) treatment for 8 weeks, followed by treatment with bovine serum albumin (BSA; 0.8 mg/kg) every three days for another 8 weeks. This regimen prevented time-dependent weight gain, regardless of diet, with 57% and 27% reductions in mice fed a standard chow (Std Chow) or PC diet, respectively. Further, supplementation reduced nuclear factor kappa B (NF-κB) activation by 2.8-fold (p = 0.0328) in PC-fed mice, consistent with albumin’s known anti-inflammatory properties. Unexpectedly, albumin also reduced hepatic neutral lipid accumulation and circulating non-esterified fatty acids. While PC-fed mice did not exhibit full progression to MASH, albumin treatment significantly increased hepatic matrix metalloproteinase-2 expression, suggesting the inhibition of early fibrotic signalling. While further studies are needed to elucidate the underlying mechanisms, these findings offer new insight into the potential of albumin, either alone or in combination with other therapies, to reduce hepatic steatosis in MASLD. Full article

This study conducts a numerical simulation of the working performance of a single horizontal shaft forced mixer using the Discrete Element Method (DEM). It systematically investigates the effects of blade installation angle, feeding method, mixing speed, and coarse aggregate particle size on the mixing uniformity. A 1:2 scale model was developed, incorporating Newton’s laws of motion and a soft-sphere contact model to simulate the particle trajectories and interactions during mixing. The results indicate that top–bottom feeding enhances mixing efficiency significantly by forming vertical convective circulation, achieving a mixing uniformity above 0.9. A moderate rotation speed of 30 rpm provides the best balance between energy consumption and mixing performance. As the coarse aggregate size increases (from 9 mm to 15 mm), the enhanced particle inertia leads to a decrease in mixing uniformity (from 0.9 to 0.6). Additionally, the discrepancy between simulation and experimental results is less than 0.1, validating the reliability of the model. This research offers theoretical guidance for the structural optimization and parameter selection of single-shaft mixers, contributing to improved mixing efficiency and concrete quality in engineering applications. Full article

During the 10th to 12th centuries, the Song, Liao, and Goryeo Dynasties and Japanese regimes in East Asia engaged in frequent activities of requesting and granting the Chinese Tripitaka (the Chinese Buddhist Canon), forming a distinctive diplomatic phenomenon termed “Tripitaka Diplomacy”. This paper examines the political and cultural dynamics underlying these cross-border interactions by analyzing the historical records of such activities among the polities. It also explores the multifaceted role of the Tripitaka in East Asian international relations, which transcended its religious significance to shape diplomatic strategies and power dynamics. Through this lens, this paper reveals the complexity of the East Asian international order during this period, emphasizing how the circulation of the Tripitaka served as both a cultural bridge and a tool for political negotiation. Full article

This study investigates the impact of rural digital economy development on agricultural carbon emission efficiency, aiming to elucidate the intrinsic mechanisms and pathways through which digital technology enables low-carbon transformation in agriculture, thereby contributing to the achievement of agricultural carbon neutrality goals. Based on provincial-level panel data from China spanning 2011 to 2022, this study examines the relationship between the rural digital economy and agricultural carbon emission efficiency, along with its underlying mechanisms, using bidirectional fixed effects models, mediation effect analysis, and Spatial Durbin Models. The results indicate the following: (1) A significant N-shaped-curve relationship exists between rural digital economy development and agricultural carbon emission efficiency. Specifically, agricultural carbon emission efficiency exhibits a three-phase trajectory of “increase, decrease, and renewed increase” as the rural digital economy advances, ultimately driving a sustained improvement in efficiency. (2) Industrial integration acts as a critical mediating mechanism. Rural digital economy development accelerates the formation of the N-shaped curve by promoting the integration between agriculture and other sectors. (3) Spatial spillover effects significantly influence agricultural carbon emission efficiency. Due to geographical proximity, regional diffusion, learning, and demonstration effects, local agricultural carbon emission efficiency fluctuates with changes in neighboring regions’ digital economy development levels. (4) The relationship between rural digital economy development and agricultural carbon emission efficiency exhibits a significant inverted N-shaped pattern in regions with higher marketization levels, planting-dominated areas of southeast China, and digital economy demonstration zones. Further analysis reveals that within rural digital economy development, production digitalization and circulation digitalization demonstrate a more pronounced inverted N-shaped relationship with agricultural carbon emission efficiency. This study proposes strategic recommendations to maximize the positive impact of the rural digital economy on agricultural carbon emission efficiency, unlock its spatially differentiated contribution potential, identify and leverage inflection points of the N-shaped relationship between digital economy development and emission efficiency, and implement tailored policy portfolios—ultimately facilitating agriculture’s green and low-carbon transition. Full article