Search Results (1,032)

Previous studies on kafirins in sorghum (Sorghum bicolor Moench) have focused mainly on grain and sprouts grown under tropical and subtropical climate conditions, while data on the content and fractional composition of kafirins in sorghum sprouts cultivated in temperate climates are scarce. In particular, the influence of the northern growing conditions, characteristic of Central Europe, on sorghum storage proteins has not yet been described, despite the fact that sorghum is currently cultivated in Poland. This study aimed to determine the total kafirin content and the distribution of α-, β-, and γ-kafirin fractions in sprouts of white and red sorghum grown under temperate climate conditions in Poland. Six-day-old sprouts were freeze-dried and extracted using a Tris-HCl/SDS/β-mercaptoethanol buffer. Kafirin content was quantified using the Bradford assay, SDS-PAGE, and HPLC, with method validation performed for accuracy, precision, and linearity. Total kafirin content ranged from 5.5 to 7.0 g/100 g dry matter (DM), with α-kafirin as the predominant fraction (4.2–5.0 g/100 g DM), followed by β-kafirin (0.5–1.0 g/100 g DM) and γ-kafirin (0.2–0.6 g/100 g DM). Sprouts of red sorghum varieties showed significantly higher total kafirin levels and a greater proportion of the γ-fraction, which may be associated with differences in protein structural properties and could suggest potential bioactivity, as indicated by previous literature. However, no direct functional or bioactivity assays were performed in this study. Statistical analysis revealed significant differences among selected sorghum varieties in total kafirin content and the proportion of the γ fraction (p < 0.05), with α being the dominant fraction in all tested samples. These results provide, for the first time, detailed data on the kafirin composition of sorghum sprouts grown in a temperate climate and address a key gap in the literature concerning the effect of environmental conditions on sorghum storage proteins. The findings support further research on the use of sorghum sprouts as a raw material for functional foods, protein-enriched products, and animal feed under European growing conditions. Full article

Road safety remains a critical concern in Poland, where infrastructure development, motorization, urban density, and public transport patterns all influence traffic accident occurrence. This study examines road accidents over the period 2010–2024, using official statistical data from the Polish Central Statistical Office (GUS) and national transport databases. The study examines how road infrastructure, vehicle ownership, population density, and public transport usage relate to traffic accidents in Poland between 2010 and 2024. A log-linear regression model was applied to estimate the elasticities of each factor, allowing interpretation of the expected percentage change in accidents associated with a 1% change in the explanatory variables. The results show that increased density of paved roads and higher urban population density are associated with higher accident numbers, while the expansion of expressways and motorways significantly is associated with lower accident numbers. Greater motorization also increases accident risk, whereas higher public transport usage has a modest mitigating effect. Bus and trolleybus operations slightly increase exposure, while tram mileage shows a negligible decrease in accidents. Results show that paved road density and urban density tend to increase accidents, while expressways reduce them. Policy implications include the importance of balancing infrastructure development with traffic management, promoting public transport, and addressing urban density to reduce accident risk. The study highlights the need for integrated approaches to improve road safety and provides a quantitative basis for evidence-informed transportation planning. Full article

Progressive climate change and building morphology influence the specific microclimate of built-up areas. This has a fundamental role in research on energy use and thermal comfort inside buildings. Most studies using data for dynamic energy simulation are based on information collected at meteorological stations in rural areas. This can lead to erroneous predictions. The main goal of the study was to combine two simulation tools—ENVI-met for microclimate predictions around historical building layouts, and DesignBuilder for assessing indoor comfort. Illustrating the impact of input data on simulation results was conducted using three types of weather data: (1) from a field campaign, (2) from a suburban station, and (3) from the typical meteorological year. The obtained results confirm that the highest precision was achieved in analyses where information obtained at a real scale in the city centre was used as boundary conditions (field measurements: MAPE = 0.6 °C, RMSE = 0.7 °C). The next step was to estimate the thermal sensations inside the living room of the existing residential building. Thermal comfort was determined using the operative temperature as an indicator. Incorporating realistic urban weather inputs enhanced the reliability of indoor comfort modelling and provided a more accurate basis for planning thermal resilience in historic residential buildings. Full article

Background: Monozygotic twin pregnancies are at increased risk of congenital abnormalities compared to singletons. In 20% of cases, both fetuses are affected (concordance), while in 80% of cases, only one fetus is affected (discordance). This study examines the prevalence of discordance for structural defects in monochorionic (MC) twins, with normal aCGH comparative genomic hybridization (aCGH), reporting the types of detected abnormalities and their possible impact on perinatal outcomes, including the rate of single and double fetal loss before 24 weeks’ gestation and the rate of preterm birth (PB) before 32 weeks’ gestation. Methods: This was a retrospective study of discordant structural fetal anomalies in MC twin pregnancies detected at first-trimester scanning in three fetal medicine centers in Poland. Results: In the study population of 381 monochorionic twin pregnancies examined at 11–13 weeks’ gestation, 21 (5.5%) pregnancies showed discordant structural defects with normal aCGH result. The most common were cardiac defects (n = 8), followed by central nervous system (CNS) (n = 6) defects and facial anomalies (n = 3). Single or double fetal loss before 28 weeks occurred in four (19%) and two (9%) cases, respectively, and was associated with intertwin crown–rump length (CRL) discordance greater than 20% (p = 0.046). PB before 32 weeks’ gestation occurred in nine cases (47%) and was strongly associated with polyhydramnios (p = 0.001), which occurred mainly in CNS and facial defects. Conclusions: The prevalence of discordant structural defects with normal aCGH results among monochorionic twins is approximately 5%. In pregnancies with discordant defects, cardiac defects are the most common. Intertwin discordance greater than than 20% is associated with an increased risk of fetal demise. Full article

Biogas production from agricultural residues is a promising solution for renewable energy production, improved waste management, and beneficial impact on climate change mitigation. The aim of this study is to assess the actual use and theoretical potential of agricultural biogas produced from animal manure in Poland at the local level. The potential and actual use of agricultural biogas are presented regionally (16 voivodeships) and locally (314 districts). The theoretical potential of agricultural biogas was estimated based on data from the Agricultural Census conducted by the Central Statistical Office in Poland in 2020. Actual biogas production is based on data from the Register of Agricultural Biogas Producers maintained by the National Support Center for Agriculture. The study shows that Poland is only tapping into the existing potential for agricultural biogas production to a limited extent. Furthermore, both actual agricultural biogas production and the identified theoretical potential vary spatially (greater potential in the northern part of the country, significantly lower in the southern part). This situation is attributed to existing barriers that hinder the utilization of existing potential. Therefore, it is crucial to seek new solutions to reduce existing barriers of an organizational, legal, technical, economic, environmental, spatial, and social nature. Full article

The cornea is essential for proper ocular function, yet its histological structure varies considerably among animal species. Of particular importance are the palisades of Vogt in the limbal region, as they serve as a niche for limbal epithelial stem cells involved in corneal epithelial regeneration. This study was conducted on 73 eyeballs collected from 18 species of non-human primates originating from the Wrocław Zoological Garden (Poland). Eyeballs were fixed, processed, and embedded in paraffin. Four-micrometer sections were stained with Mayer’s H&E and PAS. Microscopically, the cornea showed either a four-layered pattern (anterior corneal epithelium, corneal stroma, Descemet’s membrane, posterior corneal epithelium) or a five-layered pattern when Bowman’s layer was present. A four-layered cornea occurred in the ring-tailed lemur, gray mouse lemur, Guianan squirrel monkey, Angolan colobus, and L’Hoest’s monkey, while the remaining species showed a five-layered structure with Bowman’s layer. The anterior corneal epithelium varied between species in thickness and number of cell layers (central region: 2–3 to 10–15 layers; 11.81 ± 0.43 µm to 44.23 ± 0.69 µm; peripheral region: 4–5 to 9–11 layers; 8.63 ± 2.57 µm to 42.45 ± 8.61 µm). Bowman’s layer ranged from 1.18 ± 0.01 µm to 3.22 ± 0.05 µm. The corneal stroma thickness differed markedly (237.96 ± 9.64 µm to 1438.29 ± 16.38 µm), as did Descemet’s membrane (4.92 ± 0.20 µm to 43.45 ± 0.49 µm), along with PAS reaction intensity. In the limbus, palisades of Vogt ranged from weakly to clearly developed; well-defined crypt-like structures were observed in the red-bellied lemur, red ruffed lemur, black-and-white ruffed lemur, Guianan squirrel monkey, L’Hoest’s monkey, Celebes crested macaque, and yellow baboon. The limbal epithelium also varied in thickness (5–6 to 15–17 cell layers). These results confirm distinct species-specific differences in corneal and limbal morphology that may reflect ecological conditions and functional adaptation. The presented data provides a comparative reference for veterinary ophthalmology and for studies on corneal epithelial regeneration involving limbal stem cells. Full article

Non-communicable diseases, particularly cardiovascular diseases (CVD) and metabolic syndrome (MS), remain a major challenge for primary health care (PHC). This study aimed to assess cardiometabolic risk and health behaviours in adult PHC patients using routine preventive screening. This prospective observational study included 506 adults attending routine consultations in an urban PHC centre in Poland. Preventive assessment included anthropometric measurements (body weight, height, BMI, and waist circumference), blood pressure, lipid profile, and fasting glucose levels. Health behaviours were recorded using the standardised NFZ CHUK questionnaire. The 10-year CVD risk was estimated using the SCORE2 algorithm. Multivariable logistic regression was used to identify independent factors associated with high cardiovascular risk (SCORE2 ≥ 5%) and of a composite endpoint defined as the presence of any non-optimal biochemical parameter. Nearly half of the participants had excess body weight (overweight or obesity), and more than half met criteria for central obesity. Borderline or elevated total cholesterol was found in 47% of patients, abnormal LDL in 27%, low HDL-C (<40 mg/dL) in 80% (84% when applying sex-specific cut-offs), and impaired fasting glucose or diabetes in about 12%. High SCORE2 risk (≥5%) was observed in approximately 9% of the cohort. In multivariable models, SCORE2 components (age, sex, and smoking) were, as expected, associated with high SCORE2 risk, and obesity (BMI ≥ 30 kg/m²)—a factor not included in SCORE2—was additionally associated with higher risk. Additionally, age, male sex, and obesity also predicted the presence of at least one non-optimal biochemical marker. The prevalence of high SCORE2 risk increased from 1.2% in patients with 0–1 modifiable risk factor to 25.7% in those with 4–5 factors. Lower educational attainment was associated with a higher proportion of high-risk individuals in univariate analysis. Routine preventive activities in PHC enable the identification of important lipid and glucose abnormalities and the clustering of modifiable risk factors, even in a relatively young, highly educated population. Systematic cardiovascular screening and a focus on patients with accumulated risk factors should remain a priority in PHC to enable early identification of high-risk patients and timely implementation of lifestyle and therapeutic interventions. Full article

In achieving sustainable development goals, soils play a key role in environmental protection, natural resources, and food security. Peatlands are particularly important here, as they function at the interface between terrestrial and aquatic ecosystems and store large amounts of organic matter. However, organic soils are highly susceptible to transformation and degradation; therefore, their degradation caused by, among others, drainage properties is a high risk to both the environment and agriculture—it disrupts the ecosystems, causes greenhouse gas emissions, and eutrophicates the hydrosphere. Soil degradation in drained peatlands is associated with the transformation of soil organic matter (SOM), which in organic soils is the dominant component of the solid phase of the soil. The aim of our study was to assess the properties and degree of organic matter transformation in drained temperate peatland soils, with particular emphasis on sequential fractionation of SOM and humic acid properties. Due to the fact that in Poland, as many as 90% of non-forest peat bogs have been drained, we compare the mursh horizons that formed after peat bog drainage with the peat horizons that constitute the parent rock (where anaerobiosis occurs and morphological changes in the soil material are absent due to peat bog drainage). Studies were conducted on 11 soil profiles located in central-eastern Poland. Basic physicochemical soil properties were determined: pH, bulk density, contents of ash, SOM, total carbon (TC), and total nitrogen (TN). Sequential carbon fractionation was used to qualitatively analyze organic matter, which allowed for the identification of labile fractions, lipid fractions, humic substances (fulvic and humic acids), and residual fractions. Humic acids (HAs) were extracted using the Schnitzer method and analyzed for their elemental composition and spectrometric parameters in the VIS range. It was demonstrated that SOM transformation in drained temperate peatland soils was correlated with comprehensive changes in the soil’s physical and chemical properties. Compared to peat horizons, topsoil horizons were characterized by higher ash content and density, lower SOM content, and a lower TC/TN ratio. Qualitative SOM transformation during aerobic SOM transformation after draining the studied peatlands consisted of an increase in the amount of labile fractions and humic substances and a decrease in the lipid and residual fractions. The research results have shown that the HAs properties depended on the depth. HAs from topsoil horizons, compared to peat horizons, were characterized by a lower “degree of maturity,” as reflected by the values of atomic ratios (H/C, O/C) and absorbance coefficients (A_4/6 and ΔlogK). It was found that the share of the distinguished SOM fractions and HAs properties were closely correlated with the physical and chemical properties of the soils. The study demonstrated the usefulness of the sequential carbon fractionation method for assessing the effects of dewatered peat transformation. The obtained results could contribute to the development of good practices ensuring high quality of organic matter and stability of ecosystems, as well as to the development of methods for limiting the mineralization of organic matter (SOM), greenhouse gas emissions, and the loss of organic soils in agricultural areas. Full article

The aim of the experiment was to assess the effects of municipal sewage sludge, mushroom substrate, and hydrogel on the quality of energy grass species and their biomass yield. The experiment was conducted in the climatic conditions of central-eastern Poland between 2020 and 2022. Two perennial grass species were used: Miscanthus giganteus (giant miscanthus) M 19 and Panicum virgatum L. (rod millet) var. Northwind. Sewage sludge and mushroom substrate doses, each corresponding to 170 kg N·ha⁻¹, were applied in the spring of the first year. The experiment was established on microplots with four replications. Each year, biomass was harvested in January, and the yield of fresh and dry matter was determined. Then plant material was adequately prepared, and the total content of P, Ca, and Mg was measured with the ICP-OES method. The application of hydrogel resulted in a significant increase in the yield of each grass species: giant miscanthus by 11.87% and rod millet by 8.28%. Organic waste applied in combination with hydrogel increased the yield of energy plants and improved their chemical composition. Full article

This study analyses the dynamics of the energy transition within the SPRING-F group (Spain, Poland, Romania, Italy, the Netherlands, Germany, France) through a hybrid approach that combines econometric panel ARDL models with machine learning algorithms. The analysis is based on energy, economic, and technological indicators, including renewable energy consumption, energy intensity, CO₂ emissions, GDP per capita, urbanization, trade openness, and R&D expenditure. The results of the exploratory analysis highlight the existence of clear structural differences between Western European and emerging Central and Eastern European economies. Based on the estimates made with the ARDL panel model, the long-term equilibrium relationships were confirmed. They indicated positive and significant effects of urbanization and economic growth on renewable energy consumption, as well as a negative impact of CO₂ emissions. Regarding the short-term effects, the error correction coefficient suggests a moderate convergence towards equilibrium. Machine learning models highlight the superiority of nonlinear approaches, and SHAP analysis confirms the dominant role of CO₂ emissions and the heterogeneity of national energy transition trajectories. Full article

The global production of dimension stones, that is, natural stones that can be processed into blocks and used as building and decorative materials, has grown steadily since the second half of the twentieth century. The rise of global markets and trade has also contributed to a rapid increase in imports of natural stones from distant locations. The introduction of dimension stones sourced from other continents can contribute significantly to geocultural heritage, defined as geological features that have acquired cultural, historical or symbolic meaning, as well as cultural elements embedded in a geological context. In the present contribution, the use of dimension stones in the city of Poznań (Poland, central Europe) is quantified. The study reveals dramatic changes in natural stone use between 1990 and 2019, with the number of dimension stone types increasing nearly threefold, and the mean distance to the stone source areas rising from 322 to 3885 km. Growing numbers and more diversified lithologies of natural stones can improve the urban landscape and contribute to the development of geotourism. On the other hand, increasing imports of dimension stones negatively affect local producers, threaten future conservation efforts, and have significant geoethical implications. Full article

Background/Purpose: Primary urethral cancer is a rare malignancy, accounting for less than 1% of all urogenital cancers. Current epidemiological data from Europe are scarce and outdated. Therefore, the analyzes and comparison of the incidence and mortality of PUC in selected European countries, with particular focus on Poland, based on the most recent available registry data, were performed. Methods: Our study is based on country-level data and is descriptive in nature. Incidence data for PUC were obtained from the national cancer registries of Poland, Latvia, Slovenia, and Hungary. Mortality data were sourced from the WHO Mortality Database. Age-standardized incidence rates were calculated for two time intervals (2000–2009 and 2010–2019). Age-standardized mortality rates for individuals aged ≥45 years were calculated using the European Standard Population (ESP2013). Trends in incidence and mortality in Poland were analyzed using a five-year moving average. Results: The highest incidence of PUC was observed in Hungary, while Poland showed the lowest incidence. Latvia had the highest ASMRs for both sexes, whereas Poland and Greece reported the lowest mortality rates. Despite slight annual fluctuations, the overall PUC mortality rate in Poland has remained stable. Our study is limited by the relatively short analyzed period (2000–2021), restricted availability of C68.0 incidence data from national cancer registries, and incomplete mortality data in the WHO mortality database. Conclusions: This first contemporary comparative analysis of PUC epidemiology in Europe highlights the rarity of this malignancy and the limited data availability. Based on the knowledge drawn from the literature presented in the article on the impact of centralization on the increase in overall survival and the decrease in mortality in rare cancers, the authors believe that centralization of care can improve PUC patient outcomes. Full article

Sunshine duration (SD) is an essential meteorological variable. It represents the sum of time for which direct solar radiation with an intensity above 120 W∙m⁻² reaches the Earth’s surface. In the contemporary observational routine, automatic electronic devices are in use. The pyranometric method based on global solar radiation measurements (K_glob) is also proposed by the WMO to assess SD. The aim of the paper is to study the accuracy of the Slob–Monna method (SD-WMO), recommended by the WMO to calculate sunshine duration. Alternatively, the author’s method, which is based on the Ångström clearness index (SD-ACI), was used to approximate SD. For this purpose, a two-year series of SD and K_glob observations at four locations in Poland (well representing the Central European transitional climate zone) was analyzed. The result shows that, for SD-WMO, sunshine duration values are on average 16% higher than observed ones. For the SD-ACI method, they are only 5% higher. When verifying the accuracy of SD-WMO and SD-ACI approximations, we have found that, both for daily and monthly periods, the calculated SD sums are closer to the observed ones in the case of SD-ACI than for the SD-WMO method. The correlation coefficients are, respectively, 0.98 and 0.82 for daily sums and 0.99 and 0.88 for monthly sums. Full article

This article provides an in-depth overview of mine gas emission monitoring practices in the Ostrava-Karviná Coalfield (OKR), one of the most significant regions in Central Europe affected by post-mining methane leakage. The study presents field measurement techniques, including atmogeochemical surveys, systematic methane screening in soil air, and surface emission rate monitoring using accumulation chambers. Over the course of several long-term projects, more than 43 km² of land were surveyed, and risk classification maps were developed based on measured methane concentrations and surface release rates. These data support land-use planning, the design of degasification measures, and the verification of their effectiveness. Results confirm that methane emissions persist even decades after mine closures and vary depending on atmospheric pressure and local geological conditions. The OKR methodology was also compared to international practices in Poland, Canada, and China. The article concludes with future research directions focused on automation, integration of sensor networks, and predictive modeling of gas migration in post-mining environments. Full article

Green fintech merges sustainable finance with data-intensive innovation, but national translations of EU rules can create regulatory risk. This study examines how such risk manifests in Central Europe and which policy tools mitigate it. We develop a three-dimension framework—regulatory clarity and scope, supervisory consistency, and innovation facilitation—and apply a comparative qualitative design to Hungary, Slovakia, Czechia, and Poland. Using a common EU baseline, we compile coded national snapshots from primary legal texts, supervisory documents, and recent scholarship. Results show material cross-country variation in labelling practice, soft-law use, and testing infrastructure: Hungary combines central-bank green programmes with an innovation hub/sandbox; Slovakia aligns with ESMA and runs hub/sandbox, though the green-fintech pipeline is nascent; Czechia applies a principles-based safe harbour and lacks a national sandbox; and Poland relies on a virtual sandbox and binding interpretations with limited soft law. These choices shape approval timelines, retail penetration, and cross-border portability of green-labelled products. We conclude with a policy toolkit: labelling convergence or explicit safe harbours, a cross-border sandbox federation, ESRS/ESAP-ready proportionate disclosures, consolidation of recurring interpretations into soft law, investment in suptech for green-claims analytics, and inclusion metrics in sandbox selection. Full article