Search Results (4,697)

Background/Objectives: The diminished efficacy of azoles in treating fungal infections is attributed to the emergence of resistance among pathogenic fungi. Employing a synergistic approach with other compounds to enhance the antifungal activity of azoles has shown promise, yet the availability of clinically valuable adjuvants for azoles and allylamines remains limited. Studies have demonstrated that the human host environment provides multiple carbon sources, which can influence the susceptibility of C. albicans to antifungal agents. Therefore, a comprehensive investigation into the mechanisms by which carbon sources modulate the susceptibility of C. albicans to azoles may uncover a novel pathway for enhancing the antifungal efficacy of azoles. Methods: This study explored the impact of various carbon sources on the antifungal efficacy of azoles through methodologies including minimum inhibitory concentration (MIC) assessments, super-MIC growth (SMG) assays, disk diffusion tests, and spot assays. Additionally, the mechanism by which galactose augments the antifungal activity of azoles was investigated using a range of experimental approaches, such as gene knockout and overexpression techniques, quantitative real-time PCR (qRT-PCR), Western blot analysis, and cycloheximide (CHX) chase experiments. Results: This study observed that galactose enhances the efficacy of azoles against C. albicans by inhibiting the translation of Erg1. This results in the suppression of Erg1 protein levels and subsequent inhibition of ergosterol biosynthesis in C. albicans. Conclusions: In C. albicans, the translation of Erg1 is inhibited when galactose is utilized as a carbon source instead of glucose. This novel discovery of galactose’s inhibitory effect on Erg1 translation is expected to enhance the antifungal efficacy of azoles. Full article

The carbon dioxide spillover effects and influencing factors of the “Belt and Road Initiative” (BRI) in African countries must be assessed to evaluate the effectiveness, promote low-carbon transmissions in African countries, and provide recommendations for achieving the 2030 Sustainable Development Goals. This novel study employs carbon dioxide emission intensity (CEI) and per capita carbon dioxide emissions (PCE) as dual indicators to evaluate the spatial spillover effects of 54 BRI African countries on their neighboring countries’ carbon emissions from 2007 to 2023. It identifies the key factors and mechanisms affecting these spillover effects using the spatial differences-in-differences (SDID) model. Results indicate that since the launch of the BRI, the CEI and PCE of BRI African countries have significantly increased, largely due to trade patterns and industrialization structures. Greater trade openness has further boosted local economic development, thereby increasing carbon dioxide’s spatial spillover. Government management and corruption control levels show some heterogeneity in the spillover effects, which may be attributed to long-standing issues of weak institutional enforcement in Africa. Overall, this study reveals the complex relationship between BRI African economic development and environmental outcomes, highlighting the importance of developing sustainable development strategies and establishing strong differentiated regulatory regimes to effectively address environmental challenges. Full article

With the advance of digital transformation, the assessment of the environmental impact of digital tools and technologies grows more relevant. Considering the inflated expectations of environmental responsibility in higher education, this study analyses how websites of Polish universities conform to sustainable web design criteria. The sustainability audit employed a methodology encompassing carbon emissions measurement, technical website analysis, and SEO evaluation. The author analysed 63 websites of public universities in Poland using seven independent audit tools, including an original AI Custom GPT agent preconfigured in the ChatGPT ecosystem. The results revealed a substantial differentiation in CO₂ emissions and website optimisation, with an average EcoImpact Score of 66.41/100. Nearly every fourth website exhibited a significant carbon footprint and excessive component sizes, which indicates poor asset optimisation and energy-intensive design techniques. The measurements exposed considerable variability in emission intensities and resource intensity among the university websites, suggesting the need for standardised digital sustainability practices. Regulations on the carbon footprint of public institutions’ websites and mobile applications could become vital strategic components for digital climate neutrality. Promoting green hosting, “Green SEO” practices, and sustainability audits could help mitigate the environmental impact of digital technologies and advance sustainable design standards for the public sector. The proposed auditing methodology can effectively support the institutional transition towards sustainable management of digital infrastructure by integrating technical, sustainability, and organisational aspects. Full article

Poly(vinyl chloride) (PVC) undergoes thermal degradation during processing and operation, which necessitates the use of effective thermal stabilizers. The purpose of this work is to comprehensively evaluate the potential of new hierarchically structured titanium phosphates (TiP) with controlled morphology as thermal stabilizers of plasticized PVC, focusing on the effect of morphology and Ti/P ratio on their stabilizing efficiency. The thermal stability of the compositions was studied by thermogravimetric analysis (TGA) in both inert (Ar) and oxidizing (air) atmospheres. The effect of TiP concentration and its synergy with industrial stabilizers was analyzed. An assessment of the key degradation parameters is given: the temperature of degradation onset, the rate of decomposition, exothermic effects, and the carbon residue yield. In an inert environment, TiPMSI/TiPMSII microspheres demonstrated an optimal balance by increasing the temperature of degradation onset and the residual yield while suppressing the rate of decomposition. In an oxidizing environment, TiPR rods and TiPMSII microspheres provided maximum stability, enhancing resistance to degradation onset and reducing the degradation rate by 10–15%. Key factors of effectiveness include ordered morphology (spheres, rods); the Ti-deficient Ti/P ratio (~0.86), which enhances HCl binding; and crystallinity. The stabilization mechanism of titanium phosphates is attributed to their high affinity for hydrogen chloride (HCl), which catalyzes PVC chain scission, a catalyst for the destruction of the PVC chain. The unique microstructure of titanium phosphate provides a high specific surface area and, as a result, greater activity in the HCl neutralization reaction. The formation of a sol–phosphate framework creates a barrier to heat and oxygen. An additional contribution comes from the inhibition of oxidative processes and the possible interaction with unstable chlorallyl groups in PVC macromolecules. Thus, hierarchically structured titanium phosphates have shown high potential as multifunctional PVC thermostabilizers for modern polymer materials. Potential applications include the development of environmentally friendly PVC formulations with partial or complete replacement of toxic stabilizers, the optimization of thermal stabilization for products used in aggressive environments, and the use of hierarchical TiP structures in flame-resistant and halogen-free PVC-based compositions. Full article

The mining industry (MI) in mineral-rich regions is pivotal for economic growth but is challenged by significant pollution and emissions. This study examines Guangxi, a representative region in China, in light of the country’s “Dual Carbon” goals. We quantified carbon emissions from the MI from 2005 to 2021, employing the generalized Divisia index method (GDIM) to analyze the factors driving these emissions. Additionally, a system dynamics (SD) model was developed, integrating economic, demographic, energy, environmental, and policy variables to assess decarbonization strategies and the potential for carbon decoupling. The key findings include the following: (1) Carbon accounting analysis reveals a rising emission trend in Guangxi’s MI, predominantly driven by electricity consumption, with the non-ferrous metal mining sector contributing the largest share of total emissions. (2) The primary drivers of carbon emissions were identified as economic scale, population intensity, and energy intensity, with periodic fluctuations in sector-specific drivers necessitating coordinated policy adjustments. (3) Scenario analysis showed that the Emission Reduction Scenario (ERS) is the only approach that achieves a carbon peak before 2030, indicating that it is the most effective decarbonization pathway. (4) Between 2022 and 2035, carbon decoupling from total output value is projected to improve under both the Energy-Saving Scenario (ESS) and ERS, achieving strong decoupling, while the resource extraction shows limited decoupling effects often displaying an expansionary connection. This study aims to enhance the understanding and promote the advancement of green and low-carbon development within the MI in mineral-rich regions. Full article

This study develops an integrated environmental–economic assessment framework to evaluate the life cycle environmental impacts and economic costs of CO₂ geological storage and produced water treatment in saline formations in China. Using a case study of a saline aquifer carbon storage project in the Ordos Basin, eight full-chain carbon capture, utilization, and storage (CCUS) scenarios were analyzed. The results indicate that environmental and cost performance are primarily influenced by technology choices across carbon capture, transport, and storage stages. The scenario employing potassium carbonate-based capture, pipeline transport, and brine reinjection after a reverse osmosis treatment (S5) achieved the most balanced outcome. Breakeven analyses under three carbon price projection models revealed that carbon price trajectories critically affect project viability, with a steadily rising carbon price enabling earlier profitability. By decoupling CCUS from power systems and focusing on unit CO₂ removal, this study provides a transparent and transferable framework to support cross-sectoral deployment. The findings offer valuable insights for policymakers aiming to design effective CCUS support mechanisms under future carbon neutrality targets. Full article

With the rapid pace of urbanization, cities are increasingly facing severe challenges related to environmental pollution, ecological degradation, and climate change. Extreme climate events—such as heatwaves, droughts, heavy rainfall, and wildfires—have intensified public concern about sustainability, environmental protection, and low-carbon development. Ensuring environmental preservation while maintaining residents’ quality of life has become a central focus of urban governance. In this context, evaluating green indicators and predicting urban greenness is both necessary and urgent. This study incorporates international frameworks such as the EU Green City Index, the European Green Capital Award, and the United Nations Sustainable Development Goals to assess urban sustainability. The Extreme Gradient Boosting (XGBoost) algorithm is employed to predict the green level of cities and to develop multiple optimized models. Comparative analysis with traditional models demonstrates that XGBoost achieves superior performance, with an accuracy of 0.84 and an F1-score of 0.81. Case study findings identify “Greenhouse Gas Emissions per Person” and “Per Capita Emissions from Transport” as the most critical indicators. These results provide practical guidance for policymakers, suggesting that targeted regulations based on these key factors can effectively support emission reduction and urban sustainability goals. Full article

Liposomes (LPs) represent one of the most effective nanoscale platforms for drug delivery in cancer therapy due to their favorable pharmacokinetic and various body tissue compatibility profiles. Building on recent findings showing that carbon dots derived from N-hydroxyphthalimide (CDs-NHF) possess intrinsic antitumor activity, herein, we investigate the possibility of preparing complex nano-platforms composed of LPs encapsulating CDs-NHF and/or doxorubicin (DOX) for breast and lung cancer. Various LP formulations were prepared and characterized using Cryo-TEM and Cryo-SEM for morphological analysis, while zeta potential and fluorescence assessments confirmed their stability and optical properties. Cellular effects were evaluated through immunofluorescence microscopy and proliferation assays. LPs-CDs-NHF significantly reduced cancer cell viability at lower concentrations compared to free CDs-NHF, and this effect was further amplified when combined with doxorubicin. Mechanistically, the liposomal formulations downregulated key signaling molecules including pAKT, pmTOR, and pERK, indicating the disruption of cancer-related pathways. These findings suggest that LPs containing CDs-NHF, either alone or in combination with DOX, exhibit synergistic antitumor activity and hold strong promise as multifunctional nanocarriers for future oncological applications. Full article

In the context of global climate governance and the green transition, digital infrastructure serves as a critical enabler of resource allocation in the digital economy, offering strategic value in tackling synergistic pollution and carbon reduction challenges. Using panel data from 280 prefecture-level cities, this study employs a multiperiod difference-in-differences (DID) approach, leveraging smart city pilot policies as a quasinatural experiment, to assess how digital infrastructure affects urban synergistic pollution-carbon mitigation (SPCM). The empirical results show that digital infrastructure increases the urban SPCM index by 1.5%, indicating statistically significant effects. Compared with energy and income effects, digital infrastructure can influence this synergistic effect through indirect channels such as the energy effect, economic agglomeration effect, and income effect, with the economic agglomeration effect accounting for a larger share of the total effect. Additionally, fixed-asset investment has a nonlinear moderating effect on this relationship, with diminishing marginal returns on emission reduction when investment exceeds a threshold. Heterogeneity tests reveal greater impacts in eastern, nonresource-based, and environmentally regulated cities. This study expands the theory of collaborative environmental governance from the perspective of new infrastructure, providing a theoretical foundation for establishing a long-term digital technology-driven mechanism for SPCM. Full article

Asphalt plant reclaimed powder is a common solid waste in road engineering. Reusing reclaimed powder as filler holds significant importance for environmental protection and resource conservation. The key factors affecting the feasibility of reclaimed powder reuse are its acidity/alkalinity and cleanliness. Traditional evaluation methods, such as the methylene blue test and plasticity index, can assess reclaimed powder properties to guide its recycling. However, these methods suffer from inefficiency, strong empirical dependence, and high variability. To address these limitations, this study proposes a rapid and precise evaluation method for reclaimed powder properties based on Fourier transform infrared spectroscopy (FTIR). To do so, five field-collected reclaimed powder samples and four artificial samples were evaluated. Scanning electron microscopy (SEM), X-ray fluorescence spectroscopy (XRF), and X-ray diffraction (XRD) were employed to characterize their microphase morphology, chemical composition, and crystal structure, respectively. Subsequently, FTIR was used to establish correlations between key acidity/alkalinity, cleanliness, and multiple characteristic peak intensities. Representative infrared characteristic peaks were selected, and a quantitative functional group index (Is) was proposed to simultaneously evaluate acidity/alkalinity and cleanliness. The results indicate that reclaimed powder primarily consists of tiny, crushed stone particles and dust, with significant variations in crystal structure and chemical composition, including calcium carbonate, silicon oxide, iron oxide, and aluminum oxide. Some samples also contained clay, which critically influenced the reclaimed powder properties. Since both filler acidity/alkalinity and cleanliness are affected by clay (silicon/carbon ratio determining acidity/alkalinity and aluminosilicate content affecting cleanliness), this study calculated four functional group indices based on FTIR absorption peaks, namely the Si-O-Si stretching vibration (1000 cm⁻¹) and the CO₃²⁻ asymmetric stretching vibration (1400 cm⁻¹). These indices were correlated with conventional testing results (XRF for acidity/alkalinity, methylene blue value, and pull-off strength for cleanliness). The results show that the Is index exhibited strong correlations (R² = 0.89 with XRF, R² = 0.80 with methylene blue value, and R² = 0.96 with pull-off strength), demonstrating its effectiveness in predicting both acidity/alkalinity and cleanliness. The developed method enhances reclaimed powder detection efficiency and facilitates high-value recycling in road engineering applications. Full article

Modern agriculture relies heavily on the use of pesticides, with one-third of them being herbicides. Chloroacetamides are the most widely used herbicides because of their high effectiveness, but their extensive use poses environmental challenges and threatens the health of living organisms due to toxicity risks. Since the pharmacokinetic behavior and toxicity of a compound are influenced by its lipophilicity, this essential physicochemical parameter for disubstituted chloroacetamides was determined in silico and experimentally through thin-layer chromatography on reversed phases (RPTLC C18/UV254s) in mixtures of water and distinct organic modifiers. The pharmacokinetic profile of chloroacetamides was analyzed by using the BOILED-Egg model. The correlation between the obtained chromatographic parameters and software-based lipophilicity, pharmacokinetic, and ecotoxicity predictors of the studied chloroacetamides was assessed by using linear regression, but more comprehensive insight was obtained through multivariate methods—Cluster Analysis and Principal Component Analysis. It was observed that the total number of carbon atoms in the structure of their molecules, along with the type of hydrocarbon substituents, are the most important factors affecting lipophilicity, pharmacokinetics, and potential toxicity to non-target organisms. Full article

Concern over the presence of pharmaceutical waste in the environment has prompted research into the management of emerging organic micropollutants (EOMs). In response, sustainable technologies have been applied as alternatives to reduce the effects of these contaminants. This study investigated the capacity of filamentous fungi isolated from iron mine soil in the Amazon region to biodegrade the drug chloroquine diphosphate. An initial screening assessed the growth of four fungal strains on solid media containing chloroquine diphosphate: Trichoderma pseudoasperelloides CBMAI 2752, Penicillium rolfsii CBMAI 2753, Talaromyces verruculosus CBMAI 2754, and Penicillium sp. cf. guaibinense CBMAI 2758. Among them, Penicillium sp. cf. guaibinense CBMAI 2758 was selected for further testing in liquid media. A Box–Behnken factorial design was applied with three variables, pH (5, 7, and 9), incubation time (5, 10, and 15 days), and chloroquine diphosphate concentration (50, 75, and 100 mg·L⁻¹), totaling 15 experiments. The samples were analyzed by gas chromatography–mass spectrometry (GC-MS). The most effective conditions for chloroquine biodegradation were pH 7, 100 mg·L⁻¹ concentration, and 10 days of incubation. Four metabolites were identified: one resulting from N-deethylation M1 (N4-(7-chloroquinolin-4-yl)-N1-ethylpentane-1,4-diamine), two from carbon–carbon bond cleavage M2 (7-chloro-N-ethylquinolin-4-amine) and M3 (N1,N1-diethylpentane-1,4-diamine), and one from aromatic deamination M4 (N1-ethylbutane-1,4-diamine) by enzymatic reactions. The toxicity analysis showed that the products obtained from the biodegradation of chloroquine were less toxic than the commercial formulation of this compound. These findings highlight the biotechnological potential of Amazonian fungi for drug biodegradation and decontamination. Full article

Arid and semi-arid areas serve a critical regulatory function within the global carbon cycle. Understanding the response mechanisms of vegetation productivity to variations in moisture availability represents a fundamental scientific challenge in elucidating terrestrial carbon dynamics. This study systematically disentangled the respective influences of summer surface soil moisture (RSM) and precipitation (PRE) on gross primary productivity (GPP) across arid and semi-arid regions of China from 2000 to 2022. Utilizing GPP datasets alongside correlation analysis, ridge regression, and data binning techniques, the investigation yielded several key findings: (1) Both GPP and RSM exhibited significant upward trends within the study area, whereas precipitation showed no statistically significant trend; notably, GPP demonstrated the highest rate of increase at 0.455 Cg m⁻² a⁻¹. (2) Decoupling analysis indicated a coupled relationship between RSM and PRE; however, their individual effects on GPP were not merely a consequence of this coupling. Controlling for evapotranspiration and root-zone soil moisture interference, the analysis revealed that under conditions of elevated RSM, the average increase in summer–autumn GPP (SA_GPP) was 0.249, significantly surpassing the increase observed under high-PRE conditions (−0.088). Areas dominated by RSM accounted for 62.13% of the total study region. Furthermore, examination of the aridity gradient demonstrated that the predominance of RSM intensified with increasing aridity, reaching its peak influence in extremely arid zones. This research provides a quantitative assessment of the differential impacts of RSM and PRE on vegetation productivity in China’s arid and semi-arid areas, thereby offering a vital theoretical foundation for improving predictions of terrestrial carbon sink dynamics under future climate change scenarios. Full article

Traditional Pavement Condition Index (PCI) assessments are highly resource-intensive, demanding substantial time and labor while generating significant carbon emissions through extensive field operations. To address these sustainability challenges, this research presents an innovative methodology utilizing Gene Expression Programming (GEP) to determine PCI values based on International Roughness Index (IRI) measurements from Iraqi road networks, offering an environmentally conscious and resource-efficient approach to pavement management. The study incorporated 401 samples of IRI and PCI data through comprehensive visual inspection procedures. The developed GEP model exhibited exceptional predictive performance, with coefficient of determination (R²) values achieving 0.821 for training, 0.858 for validation, and 0.8233 overall, successfully accounting for approximately 82–85% of PCI variance. Prediction accuracy remained robust with Mean Absolute Error (MAE) values of 12–13 units and Root Mean Square Error (RMSE) values of 11.209 and 11.00 for training and validation sets, respectively. The lower validation RMSE suggests effective generalization without overfitting. Strong correlations between predicted and measured values exceeded 0.90, with acceptable relative absolute error values ranging from 0.403 to 0.387, confirming model effectiveness. Comparative analysis reveals GEP outperforms alternative regression methods in generalization capacity, particularly in real-world applications. This sustainable methodology represents a cost-effective alternative to conventional PCI evaluation, significantly reducing environmental impact through decreased field operations, lower fuel consumption, and minimized traffic disruption. By streamlining pavement management while maintaining assessment reliability and accuracy, this approach supports environmentally responsible transportation systems and aligns contemporary sustainability goals in infrastructure management. Full article

Tropical forests continue to experience high levels of habitat loss and degradation, with wildfires becoming a frequent component of human-modified landscapes. Here we investigate the response of palm species to the conversion of old-growth forests to successional mosaics, including forest patches burned during wildfires. Palms (≥50 cm height) were recorded once in 2023–2024, across four habitat classes: terra firme old-growth stands, regenerating forest stands associated with slash-and-burn agriculture, old-growth stands burned once and twice, and active cassava fields, in the Tapajós-Arapiuns Extractive Reserve, in the eastern Brazilian Amazon. The flammability of palm leaf litter and forest litter were also examined to assess the potential connections between palm proliferation and wildfires. A total of 10 palm species were recorded in this social forest (including slash-and-burn agriculture and resulting successional mosaics), with positive, negative, and neutral responses to land use. Species richness did not differ among forest habitats, but absolute palm abundance was greatest in disturbed habitats. Only Attalea spectabilis Mart. (curuá) exhibited increased relative abundance across disturbed habitats, including active cassava field. Attalea spectabilis accounted for almost 43% of all stems in the old-growth forest, 89% in regenerating forests, 90% in burned forests, and 79% in crop fields. Disturbed habitats supported a five-to-ten-fold increment in curuá leaves as a measure of habitat flammability. Although curuá litter exhibited lower flame temperature and height, its lower carbon and higher volatile content is expected to be more sensitive to fire ignition and promote the spread of wildfires. The conversion of old-growth forests into social forests promotes the establishment of palm-dominated forests, increasing the potential for a forest transition further fueled by wildfires, with effects on forest resilience and social reproduction still to be understood. Full article