Search Results (57)

Against the backdrop of ecological civilization construction and regional coordinated development strategies, functional zone (MFOZ) planning guides national spatial development through differentiated policies. However, a prominent conflict exists between the ecological protection responsibilities and regional development rights in restricted and prohibited development zones, leading to a vicious cycle of “ecological protection → restricted development → poverty exacerbation”. This paper focuses on the synergistic mechanisms between ecological compensation and targeted poverty alleviation. Based on the capability approach and sustainable development goals (SDGs), it analyzes the dialectical relationship between the two in terms of goal coupling, institutional design, and practical pathways. The study finds that ecological compensation can break the “ecological poverty trap” through the internalization of externalities and the enhancement of livelihood capabilities. Nevertheless, challenges remain, including low compensation standards, unbalanced benefit distribution, and insufficient legalization. Through case studies of the compensation reform in the water source area of Southern Shaanxi, China, and the Common Agricultural Policy (CAP) of the European Union, this paper proposes the construction of a long-term mechanism integrating differentiated compensation standards, market-based fund integration, legal guarantees, and capability enhancement. The research emphasizes the need for institutional innovation to balance ecological protection and livelihood improvement, promoting a transition from “blood transfusion” compensation to “hematopoietic” development, thereby offering a Chinese solution for global sustainable development. Full article

Electroless nickel plating is a chemical deposition process in which nickel ions within a plating solution are reduced by a chemical reducing agent and subsequently deposited onto the surface of a solid substrate. Chemical nickel-plating wastewater contains substantial amounts of phosphorus as well as abundant nickel resources. In this study, electrodialysis coupled with advanced oxidation techniques was utilized for the efficient recovery of nickel and phosphorus from spent nickel-plating solutions. The end-of-life tank solution from chemical nickel plating was treated via electrodialysis to remove harmful phosphite and sulfate ions, enabling the purified solution to be reused in plating production by supplementing it with appropriate amounts of sodium hypophosphite and nickel sulfate. Subsequently, the concentrate generated from electrodialysis was treated using peroxydisulfate (PDS)-based advanced oxidation technology to break nickel complexation and simultaneously promote the oxidation of hypophosphite and phosphite ions. Finally, Ca(OH)₂ was employed as a precipitating agent to effectively recover phosphorus from the treated concentrate. From an economic perspective, optimal process conditions were determined as follows: a current density of 20 mA/cm², concentrate-to-dilute water volume ratio of 1:1, current speed of 1.0 m³/h, and a sodium sulfate concentration in concentrate of 20 g/L. Under these conditions, the migration rates of H₂PO₂⁻ and HPO₃²⁻ ions reached 67.3% and 62.53%, respectively, whereas Ni²⁺ exhibited significantly lower mobility at only 6.77%. The purified wastewater recovered approximately 60% of its initial plating activity. Regarding the concentrate—which is a by-product of electrodialysis—the hypophosphite ions were nearly completely oxidized using a PDS dosage of 0.3 mol/L. Furthermore, when the Ca/P molar ratio was adjusted to 2.0, total phosphorus (TP) and nickel (Ni) removal efficiencies exceeded 98% and 93%, respectively. Full article

Objectives: This study aims to develop a tablet press machine (TPM) integrated with machine learning (ML) and deep learning (DL) models for in-line detection of defective tablets as a Process Analytical Technology (PAT) tool. This study aimed to predict tablet defects, including capping occurrence and inappropriate tablet breaking force (TBF), using real-time processing data. Methods: Free-flowing metformin HCl (MF) granules produced using the granulation method were compressed into tablets using a TPM. Commercial-scale experiments were conducted to determine the MF tablets’ defect criteria. Random Forest (RF) and Artificial Neural Network (ANN) models were designed and trained using sensed in-line data, including compression force, ejection force, and compression speed, to predict tablet quality defects. Subsequently, the TPM was designed and manufactured for in-line PAT using an RF model. The TPM was verified by sorting defective tablets in-line using a pretrained defect-detection algorithm. Results: The RF model demonstrated the highest predictive accuracy at 93.7% with an Area Under the Curve (AUC) of 0.895, while the ANN model achieved an accuracy of 92.6% with an AUC of 0.878. The TPM successfully sorted defective tablets in real time, achieving 99.43% sorting accuracy and a defective tablet detection accuracy of 93.71%. Conclusions: These results suggest that a ML-based TPM applied during the tableting process can detect defects non-destructively during the scale-up of wet granulation. In particular, it can serve as the base TPM model for an in-line PAT process during a scale-up process that produces small batches of multiple products, thereby reducing additional labor, time, and API consumption, and decreasing environmental pollution. Full article

Background: Captopril (CAP), an angiotensin-converting enzyme inhibitor (ACEI), is widely prescribed for managing hypertension, heart failure, and related conditions. When administered orally, CAP undergoes hepatic metabolism, resulting in a bioavailability of 60–75%. However, to bypass the first-pass metabolism and other limitations of the oral route, mucoadhesive buccal films have gained attention as a promising alternative with several advantages. The aim of this work was the formulation and optimization of chitosan-ascorbate mucoadhesive films for buccal delivery of CAP for the management of a hypertension crisis (10 mg and 20 mg) by employing quality by design (QbD) principles and the design of experiment (DoE) approach. Materials and methods: In the present work, chitosan (CHI) was selected as a film-forming agent due to its permeability-enhancing properties, which could be further improved through salification with ascorbic acid (AA). The polymer films were prepared by the solvent casting method. Results: The optimized CAP-loaded formula showed appropriate in vitro mucoadhesion force (>15 N) and breaking hardness (>14 N). The different CAP-containing films had a high drug content (>95%) with homogeneous drug distribution, thus complying with the requirements of Pharmacopeia. FT-IR and RAMAN spectroscopy analyses demonstrated successful incorporation of the drug, and interaction was observed between the excipients of the films, especially in the form of hydrogen bonds. The dissolution test showed immediate release of the API with a similar release pattern from both concentrations of CAP-loaded films. Conclusions: The properties of the prepared films met the predetermined critical quality attribute requirements. The optimized formula of CHI 1.4%, AA 2.5%, and glycerol 0.3% appears to be a promising buccal drug delivery system for CAP. Full article

This paper offers a new perspective on the explanation of the poleward mass flux in the stratosphere. This mass flux represents the upper leg of the so-called Brewer–Dobson circulation. This new perspective is based on the following hypothesis. A positive potential vorticity anomaly, centered over the North Pole, exists in the stratosphere during the winter half-year. This positive potential vorticity anomaly is associated with a negative isentropic density anomaly, which forms due to cross-isentropic downwelling associated with radiative cooling. Isentropic potential vorticity mixing due to breaking planetary waves weakens this potential vorticity anomaly while zonal-mean thermal wind balance is maintained. This requires a weakening of the negative Polar cap isentropic density anomaly, which in turn requires a poleward isentropic mass flux. Support for this hypothesis is found in a case study of a major Sudden Stratospheric Warming event, as an example of intense potential vorticity mixing. It is shown that the stratosphere, both before and after this event, is very close to zonal-mean thermal wind balance, despite the disruptive potential vorticity mixing, while mass is shifted poleward during this event. Solutions of the potential vorticity-inversion equation, which is an expression of thermal wind balance, for zonal-mean potential vorticity distributions before and after the Sudden Stratospheric Warming, demonstrate that mass must shift poleward to maintain zonal-mean thermal wind balance when the positive potential vorticity anomaly is eliminated by mixing. This perspective on the reasons for the poleward stratospheric mass flux also explains the observed isobaric warming as well as the Polar cap zonal-mean zonal wind reversal during a major Sudden Stratospheric Warming. Full article

In recent years, plasma medicine has developed rapidly as a new interdisciplinary discipline. However, the key mechanisms of interactions between cold atmospheric plasma (CAP) and biological tissue are still in the exploration stage. In this study, by introducing the reactive molecular dynamics (MD) simulation, the capsid protein (CA) molecule of HIV was selected as the model to investigate the reaction process upon impact by reactive oxygen species (ROS) from CAP and protein molecules at the atomic level. The simulation results show that ground-state oxygen atoms can abstract hydrogen atoms from protein chains and break hydrogen bonds, leading to the destruction of the disulfide bonds, C–C bonds, and C–N bonds. Furthermore, the generation of alcohol-based groups resulting from the impact of ROS can alter the hydrophobicity of molecules and induce damage to the primary, secondary, and tertiary structures of proteins. The dosage effects on the reaction processes and products induced by CAP are also explored with varying numbers of ROS in the simulation box, and the influences on the broken C–H, N–H, and C–N bonds are discussed. In this study, the computational data suggest that severe damage can be caused to CA upon the impact of ROS by revealing the reaction processes and products. Full article

Background/Objectives: This study aimed to establish a predictive model for critical quality attributes (CQAs) related to tablet integrity, including tablet breaking force (TBF), friability, and capping occurrence, using machine learning-based models and nondestructive experimental data. Methods: The machine learning-based models were trained on data to predict the CQAs of metformin HCl (MF)-containing tablets using a commercial-scale wet granulation process, and five models were each compared for regression and classification. We identified eight input variables associated with the process and material parameters that control the tableting outcome using feature importance analysis. Results: Among the models, the Gaussian Process regression model provided the most successful results, with R² values of 0.959 and 0.949 for TBF and friability, respectively. Capping occurrence was accurately predicted by all models, with the Boosted Trees model achieving a 97.80% accuracy. Feature importance analysis revealed that the compression force and magnesium stearate fraction were the most influential parameters in CQA prediction and are input variables that could be used in CQA prediction. Conclusions: These findings indicate that TBF, friability, and capping occurrence were successfully modeled using machine learning with a large dataset by constructing regression and classification models. Applying these models before tablet manufacturing can enhance product quality during wet granulation scale-up, particularly by preventing capping during the manufacturing process without damaging the tablets. Full article

High-silica adakites exhibit specific compositions, as follows: SiO₂ ≥ 56 wt.%, Al₂O₃ ≥ 15 wt.%, Y ≤ 18 ppm, Yb ≤ 1.9 ppm, K₂O/Na₂O ≥ 1, MgO < 3 wt.%, high Sr/Y (≥10), and La/Yb (>10). Devonian I-type adakitic granitoids in the northern Appalachians of New Brunswick (NB, Canada) share geochemical signatures of adakites elsewhere, i.e., SiO₂ ≥ 66.46 wt.%, Al₂O₃ > 15.47 wt.%, Y ≤ 22 ppm, Yb ≤ 2 ppm, K₂O/Na₂O > 1, MgO < 3 wt.%, Sr/Y ≥ 33 to 50, and La/Yb > 10. Remarkably, adakitic intrusions in NB, including the Blue Mountain Granodiorite Suite, Nicholas Denys, Sugar Loaf, Squaw Cap, North Dungarvan River, Magaguadavic Granite, Hampstead Granite, Tower Hill, Watson Brook Granodiorite, Rivière-Verte Porphyry, Eagle Lake Granite, Evandale Granodiorite, North Pole Stream Suite, and the McKenzie Gulch porphyry dykes all have associated Cu mineralization, similar to the Middle Devonian Cu porphyry intrusions in Mines Gaspé, Québec. Trace element data support the connection between adakite formation and slab break-off, a mechanism influencing fertility and generation of porphyry Cu systems. These adakitic rocks in NB are oxidized, and are relatively enriched in large ion lithophile elements, like Cs, Rb, Ba, and Pb, and depleted in some high field strength elements, like Y, Nb, Ta, P, and Ti; they also have Sr/Y ≥ 33 to 50, Nb/Y > 0.4, Ta/Yb > 0.3, La/Yb > 10, Ta/Yb > 0.3, Sm/Yb > 2.5, Gd/Yb > 2.0, Nb + Y < 60 ppm, and Ta + Yb < 6 ppm. These geochemical indicators point to failure of a subducting oceanic slab (slab rollback to slab break-off) in the terminal stages of subduction, as the generator of post-collisional granitoid magmatism. The break-off and separation of a dense subducted oceanic plate segment leads to upwelling asthenosphere, heat advection, and selective partial melting of the descending oceanic slab (adakite) and (or) suprasubduction zone lithospheric mantle. The resulting silica-rich adakitic magmas ascend through thickened mantle lithosphere, with minimal affect from the asthenosphere. The critical roles of transpression and transtension are highlighted in facilitating the ascent and emplacement of these fertile adakitic magmas in postsubduction zone settings. Full article

In recent years, plasma medicine, as an innovative and rapidly growing field, has garnered increasing attention. Nonetheless, the fundamental mechanisms of the interaction processes of cold atmospheric plasma (CAP) and biomolecules remain under investigation. In this paper, a reactive molecular dynamic (MD) simulation with ReaxFF potential was performed to explore the interactions of reactive oxygen species (ROS) produced in CAP, exemplified by OH radicals, and four distinct oligonucleotides. The breakage of single-stranded oligonucleotides induced by OH is observed in the simulation, which could seriously influence the biological activity of cellular DNA. The base release induced by OH radicals means the loss of base sequence information, and the H-abstraction at nucleobases affects the gene strand complementarity, gene transcription, and replication. In addition, the dose effects of OH radicals on bond formation and breaking of oligonucleotides are also discussed by adjusting the number of ROS in the simulation box. This study can enhance the comprehension of interactions between CAP and DNA, thereby indicating possible improvements in plasma device optimization and operation for medical applications. Full article

To address the effect of a ship’s rolling on the fluidization quality of fluidized beds, in this study, a simulation of a rolling fluidized bed with longitudinal internal members added (R-FBLIM) was carried out and compared with that of a rolling fluidized bed without internal members added (R-FBWIM). The transient motion, as well as the behavioral characteristics of the bubbles within the R-FBLIM, was analyzed; the variation patterns of the number of bubbles, as well as the equivalent diameter of the bubbles, were compared for different apparent gas velocities, oscillation periods, and amplitudes; and the mechanism of the action of the longitudinal internal members was investigated. The results show that the structural design of the longitudinal internal members can effectively improve the gas–solid fluidization quality of the rolling fluidized bed. The horizontal support plate and the cap hole structure can effectively break the air bubbles, the cap hole structure promotes the radial mixing of the gas–solid fluid, and the internal and outer rings of the curved surface plate roll in rows, which inhibit the aggregation behavior of the gas–solid fluid to the two sides of the oscillating planes, respectively, by cooperating with the cap hole structure. Compared with R-FBWIM, the gas–solid phase within R-FBLIM is more spatially distributed, with the number of bubbles increasing by about 2–4 times and the mean diameter decreasing by about 50–60%. The number of bubbles increases with the gas velocity but decreases with the rolling amplitude; the mean diameter decreases with the gas velocity but responds less to the rolling amplitude change. Full article

This study compares the energetic and functional aspects of pneumatic and traditional disintegration methods during the vinification of Negroamaro grapes to produce ready-to-drink wine, focusing on sustainability and energy efficiency in winemaking. It addresses the critical need to reducing costs and environmental impact in the wine industry through improved energy efficiency and sustainable practices. The experimental tests conducted reveal that the pneumatic system exhibits advantages in terms of energy consumption, production time, and thermal homogenization during fermentation compared to the traditional system. Results indicate that the pneumatic system requires significantly lower energy consumption and shorter operating times during fermentation and pressing phases while maintaining consistent wine quality, highlighting its potential for more efficient and sustainable winemaking practices. Full article

Telomeres protect the ends of linear eukaryotic chromosomes from being recognized as DNA double-strand breaks. Two major protein complexes are involved in the protection of telomeres: shelterin and CST. The dysfunction of these complexes can challenge the function of telomeres and lead to telomere fusions, breakage–fusion–bridge cycles, and cell death. Therefore, monitoring telomere fusions helps to understand telomeres biology. Telomere fusions are often analyzed by Fluorescent In Situ Hybridization (FISH) or PCR. Usually, both methods involve hybridization with a telomeric probe, which allows the detection of fusions containing telomeric sequences, but not of those lacking them. With the aim of detecting both types of fusion events, we have developed a nested PCR method to analyze telomere fusions in Arabidopsis thaliana. This method is simple, accurate, and does not require hybridization. We have used it to analyze telomere fusions in wild-type and mutant plants altered in CTC1, one of the three components of the Arabidopsis CST telomere capping complex. Our results show that null ctc1-2 mutant plants display fusions between all telomeric regions present in Arabidopsis chromosomes 1, 3 and 5, thus highlighting the widespread end-capping protection achieved by CTC1. Full article

Various stressors lead to an increase in ROS and damage to plant tissues. Plants have a powerful antioxidant system (AOS), which allows them to neutralize excess ROS. We detected an intense fluorescent glow of ROS in the cells of the cap, meristem, and elongation zones in the roots of wheat Triticum aestivum (Orenburgskaya 22 variety) and Triticum durum (Zolotaya variety). An increase in ROS was accompanied by DNA breaks in the nuclei of wheat root cells, the release of cytochrome c from mitochondria into the cytoplasm, and the translocation of phosphatidylserine into the outer layer of the plasma membrane under salt stress and hypoxia. The different resistances of the two wheat varieties to different abiotic stresses were revealed. The soft wheat variety Orenburgskaya 22 showed high resistance to salt stress but sensitivity to hypoxia, and the durum wheat variety Zolotaya showed tolerance to hypoxia but high sensitivity to salt stress. Different activations of AOS components (GSH, MnSOD, Cu/ZnSOD, CAT, PX, GPX, and GST) were revealed in different wheat genotypes. The basis for the tolerance of the Zolotaya variety to hypoxia is the high content of glutathione (GSH) and the activation of glutathione-dependent enzymes. One of the mechanisms of high resistance to salt stress in the Orenburgskaya 22 variety is a decrease in the level of ROS as a result of the increased activity of the MnSOD and Cu/ZnSOD genes. Identifying the mechanisms of plant tolerance to abiotic stress is the most important task for improving breeding varieties of agricultural plants and increasing their yield. Full article

This study contributes to the ongoing debate on the size effect and size-based investment styles by investigating the return and volatility spillovers and time-varying conditional correlations among Saudi large-, mid-, and small-cap indices. To this end, we utilize the weekly returns on the MSCI Saudi large-, mid-, and small-cap indices over a long sample period, spanning several crises. The econometric approach that we use is a VAR-asymmetric BEKK-GARCH model which accounts for structural breaks. On the basis of the VAR-asymmetric BEKK-GARCH model estimation results, we calculate portfolio weights and hedge ratios, and discuss their risk management implications. The empirical results confirm the presence of unilateral return spillovers running from mid- to small-cap stocks, while multilateral volatility spillovers are documented, albeit substantially weakened when accounting for structural breaks. The time-varying conditional correlations display clear spikes around crises, which translate to higher hedge ratios, increasing the cost of hedging during turbulent times. The optimal portfolio weights suggest that investors generally overweight large caps in their portfolios during uncertain times to minimize risk without lowering expected returns. The main takeaway from our results is that passively confining fund managers to a particular size category regardless of the prevailing market conditions may lead to suboptimal performance. Full article

In the last two decades, Portugal suffered the effects of two global crises, the financial crisis and the COVID-19 pandemic, as well as the Common Agriculture Policy reforms. These crises had a great impact on the Portuguese economy, but it is completely unclear how they affected the dynamics of the Portuguese agrifood sector. This study’s objective is to analyze the resilience of this sector to European and global socks, testing the effects on international trade. Secondary data from the Portuguese Statistics Institute were used for the exports and imports trade series of animal and vegetable products and food industries from 2000 to 2020. The methodology was based on the structural xtbreak model, stability analysis, and tests for structural breaks. Some volatility was observed in the trade series, particularly in imports, without consistency among years, trade sectors, or imports versus exports trade. In the case of exports, one or two structural breaks in the different sectors occurred in different years. The most relevant dynamics occurred after the sovereign debt crisis. It was concluded that CAP reforms and global crises seem to not have caused new relevant dynamics in the Portuguese international agrifood trade. This revealed the resilience of the sector to external shocks. Full article