Search Results (13,502)

Drought stress represents a major constraint on global apple production, with the widely used semi-dwarfing rootstock ‘M.26’ being particularly vulnerable to water deficit. Although the osmolyte trimethylamine N-oxide (TMAO) has been shown to improve abiotic stress tolerance in the model plant Arabidopsis, its potential role in enhancing drought resilience in woody fruit trees remains largely unexplored. Under prolonged moderate drought stress, exogenous TMAO application significantly promoted plant growth, mitigating the drought-induced suppression of plant height by 5.3–12.2% compared to untreated drought-stressed controls and alleviating the decline in above-ground biomass. This improvement was underpinned by a substantial alleviation of root growth inhibition, with TMAO restoring total root length and biomass from 37% in the control to only 6.1–9.5%. TMAO also fine-tuned the root-to-shoot ratio to favor resource allocation to roots. Consequently, TMAO-treated plants maintained superior leaf water status, exhibiting higher relative water content (drought-induced reduction limited to ~17.5% with TMAO versus 26.3% in the control). Physiologically, TMAO alleviated the drought-induced stomatal limitation of photosynthesis, sustaining higher net photosynthetic rate, stomatal conductance, and transpiration rate. Crucially, under severe drought stress, TMAO pretreatment markedly enhanced ‘M.26’ survival rates from approximately 39% in the untreated control to 60–68%, representing a relative increase of approximately 74%. Collectively, this study demonstrates that exogenous application TMAO significantly enhances drought tolerance in apple rootstock ‘M.26’, highlighting its potential as an effective and environmentally safe plant growth regulator for more sustainable cultivation of fruit trees under irregular/erratic irrigation conditions. Full article

Growing pressure on water resources and mineral fertilizer use calls for innovative and resource-efficient agri-food systems. Aquaponics, integrating aquaculture and hydroponics, represents a promising approach for sustainable greenhouse production. This study, aiming to explore alternative water and nutrient sources for greenhouse tomato production without compromising plant adaptability or yield, evaluated the co-cultivation of grape tomato and rainbow trout in a vertical decoupled aquaponic system under controlled greenhouse conditions. Two aquaponic nutrient strategies were tested: unmodified aquaponic water (AP) and complemented aquaponic water (CAP), with conventional hydroponics (HP) as a control, in a Deep Water Culture hydroponic system. Plant performance was assessed through marketable yield and physiological parameters, while system performance was evaluated using combined-biomass Energy Use Efficiency (EUE), Freshwater Use Efficiency (fWUE) and Nitrogen Use Efficiency (NUE), accounting for both plant and fish production. CAP significantly improved tomato yield (9.86 kg m⁻²) compared to AP (2.40 kg m⁻²), although it remained lower than HP (12.14 kg m⁻²). Fresh WUE was comparable between CAP and HP (9.22 vs. 9.24 g L⁻¹), demonstrating effective water reuse. In contrast, EUE and NUE were lower in CAP, reflecting the additional energy demand of the recirculating aquaculture system and nutrient limitations of fish wastewater. These results highlight aquaponics as a water-efficient production system while emphasizing that optimized nutrient management and energy strategies are critical for improving its overall sustainability and performance. Full article

Microalgae photobioreactors (PBRs) are promising building-integrated biotechnologies for carbon capture and biomass production; however, their high energy requirements for artificial lighting remain a significant energy barrier in cold climates. This study developed an integrated spectral–optical energy modeling framework to evaluate two PBR deployment strategies in State College, PA: rooftop daylight-exposed integration and basement installation with solar-assisted lighting. Results show that fiber-optic daylighting can supply a substantial fraction of photosynthetically useful light without introducing additional internal heat loads, while photovoltaics sized at approximately 0.40–0.55 kWDC per reactor can offset the annual PBR lighting energy use when sufficient roof area is available. Whole-building energy simulations further reveal that rooftop PBR integration reduces total annual space energy consumption by ~21% relative to basement placement due to lower artificial lighting and cooling loads. When combined, PV and fiber systems can fully meet basement PBR lighting demand, whereas rooftop configurations may rely more on grid electricity. Economically, fiber-optic daylighting achieves comparable lighting offsets at roughly half the annualized cost of PV-based systems, subject to surface-area and routing constraints. Overall, solar-assisted lighting strategies markedly improve the operational sustainability of building-integrated PBRs in cold climates, with fiber-optic daylighting offering substantial spectral and thermal advantages, subject to surface-area availability and routing-related design constraints. Full article

Recycling industrial CO₂ into agricultural systems offers a dual-purpose strategy for achieving carbon neutrality and enhancing sustainable crop production. Although elevated CO₂ is known to influence plant growth, the directed delivery of industrially sourced CO₂ via drip irrigation to modulate rhizosphere processes in arid soils remains underexplored. We conducted a two-year field experiment in a Xinjiang cotton field to evaluate the effects of five concentrations of industrial CO₂ solution (0.00–0.16 kg·m⁻³) on soil properties, nutrient dynamics, and crop performance. The optimal CO₂ treatment (0.08 kg·m⁻³) significantly reduced soil pH by up to 0.3 units and electrical conductivity by up to 27.9%, while enhancing the availability of ammonium-N (51.1%), available P (8.1%), and available K (32.65%). These improved soil conditions subsequently enhanced plant N, P, and K accumulation (56.2%, 41.9%, and 53.2%, respectively), total biomass (31.8%), and seed cotton yield (5.76–6.06%). Our findings demonstrate that CO₂-enriched irrigation enhances the rhizosphere microenvironment and nutrient availability, providing a novel pathway for carbon recycling and high-efficiency cotton production in arid regions. Full article

Biomass ashes represent a promising secondary phosphorus (P) source, yet their agronomic performance depends on feedstock origin, processing, and crop-specific interactions. This study evaluated the P fertilizer efficacy of raw and processed biomass ashes derived from cereal straw and paludiculture biomass, compared with triple superphosphate (TSP), using two sequential greenhouse pot experiments with maize, amaranth, and blue lupine. Processed ash products, particularly compacted ashes and ash–straw mixtures, increased plant biomass and P uptake to levels comparable to or exceeding those achieved with TSP. The cumulative P uptake of the three crops reached up to 250–300 mg pot⁻¹ under processed ash treatments, exceeding the uptake under TSP (≈150–180 mg pot⁻¹) and the unfertilized control (≤80 mg pot⁻¹). However, crop-specific differences were observed: amaranth benefited most from the ash products, whereas combinations of ashes with lupine were less favorable. Beside acting as a P source, processed biomass ashes also increased soil pH by about 0.5 units, improved soil aggregation by increasing macroaggregates (>2 mm) to up to 20% compared with only about 7% in TSP and the control, and promoted favorable shifts in Hedley P fractions. Soil enzyme activities were governed primarily by crop species, with amaranth stimulating phosphatase activity the most. Further research should aim to refine crop-specific application strategies for processed biomass ashes and to elucidate their impacts on soil structure and P dynamics. Full article

Agriculture faces increasing challenges in ensuring food security under a changing climate, where abiotic stresses such as salinity and drought represent major constraints to crop productivity. These stresses induce complex physiological and biochemical alterations in plants, including osmotic imbalance, oxidative damage, and disruption of metabolic pathways, ultimately impairing growth and yield. In this context, the application of biostimulants has emerged as a sustainable strategy to enhance plant resilience. While synthetic products are widely available, growing attention is being directed toward natural bio-based products, particularly those derived from renewable biomasses and organic wastes, in line with circular economy principles. This review critically examines the current literature on bio-based products with biostimulant properties, with particular emphasis on vermicompost-derived extracts, humic-like substances, and macro- and microalgae extracts, focusing on their role in mitigating salt and drought stress in plants. The reviewed studies consistently demonstrate that these bio-products enhance plant tolerance to abiotic stress by modulating key physiological and biochemical processes, including hormonal regulation, activation of antioxidant defence systems, accumulation of osmoprotectants, and regulation of secondary metabolism. Moreover, evidence indicates that these bio-based inputs can improve nutrient use efficiency, photosynthetic performance, and overall plant growth under stress conditions. Overall, this review highlights the potential of non-microbial bio-based biostimulants as effective and sustainable tools for climate-resilient agriculture, while also underlining the need for further research to standardize formulations, clarify mechanisms of action, and validate their performance under field conditions. Full article

Biofilm formation by non-C. albicans Candida (NAC) species is a major factor in device-associated infections, yet few studies have examined their development under physiologically relevant conditions. This study evaluated the biofilm-forming capacity of Candida tropicalis, Candida parapsilosis sensu stricto and Candida albicans on stainless steel surfaces in the presence of artificial saliva, simulating the respiratory tract environment of tracheostomized patients. Standardized inocula were incubated for 24 h, and biofilms were assessed through quantification of viable cells, biomass, biofilm matrix production and structural characterization by scanning electron microscopy (SEM). C. tropicalis produced the most robust biofilms compared to C. albicans and C. parapsilosis stricto sensu isolates, with significantly higher biomass and biofilm matrix (p < 0.001). C. parapsilosis sensu stricto developed less dense yet structurally defined biofilm networks. SEM confirmed mature and compact biofilm architecture, especially in C. tropicalis. These results demonstrate the strong intrinsic biofilm-forming ability of NAC species on stainless steel under host-like conditions, reinforcing their capacity to persist on medical surfaces and their relevance as independent contributors to biofilm-related contamination and infection. Full article

This study optimizes fertilization schemes through the emergy analysis of different nutrient reduction treatments in maize cropping ecosystems in Xinjiang, thereby providing technical support for improving chemical fertilizer use efficiency and maintaining the stability of farmland ecosystems. The study was conducted in 2024 at Huaxing Farm in Changji Hui Autonomous Prefecture, Xinjiang Uyghur Autonomous Region. The experiment used the local conventional nitrogen and phosphorus fertilization rates as the control treatment N0P0 (applying P 183 kg·hm⁻² and N 246 kg·hm⁻²), with eight different N and P nutrient reduction treatments: N0P1 (10% reduction in P only), N0P2 (20% reduction in P only), N1P0 (10% reduction in N only), N2P0 (20% N reduction), N1P1 (10% N and P reduction), N1P2 (10% N and 20% P reduction), N2P1 (20% N and 10% P reduction), and N2P2 (20% N and P reduction). Each treatment was replicated three times. Based on biomass data of maize plant components under different fertilization treatments, emergy analysis of farmland ecosystems and integration of economic benefit indicators led to the optimization of an optimal fertilization scheme. Results indicate that the N0P1 treatment performed optimally: maize plant biomass reached 251.09 g, significantly higher than other treatments. The N0P1 treatment exhibited the highest energy output (3.23 × 10¹⁶ sej·hm⁻²), the highest net energy yield ratio (EYR) of 1.45, and an energy sustainability index (ESI) of 3.34, representing a high level. It also delivered the highest economic benefit, with a net profit of 8571.95 CNY·hm⁻² and a production–investment ratio of 1.71. In conclusion, the N0P1 treatment (10% reduction in phosphorus alone) demonstrated superior performance in biomass yield, energy utilization efficiency, ecological sustainability, and economic benefits, making it the optimal fertilization strategy for maize fields in this region. Full article

Mechanical deep fertilization is an efficient fertilization method. However, the effects of different types of nitrogen fertilizer on rice grain yield and nitrogen use efficiency under deep-application conditions remain unclear. In this study, field experiments were carried out in 2021 and 2022. The experimental treatments consisted of three types of nitrogen fertilizer, i.e., urea (T1), slow/controlled-release fertilizer (T2), and super rice special fertilizer (T3), applied at a rate of 150 kg N ha⁻¹ via mechanical deep placement using Meixiangzhan 2 (MX) and Y liangyou 1378 (YL) as experimental materials. No fertilizer application was used as a control (T0) to calculate nitrogen use efficiency. The T2 treatment produced 29.03% and 25.52% higher grain yield for MX and YL because of the increase in productive panicles per ha and spikelet number per panicle, 21.20% and 13.68% higher nitrogen recovery efficiency, and 24.57% and 23.29% higher nitrogen agronomy efficiency than T1, respectively. In addition, the T2 treatment significantly improved the leaf area index and total aboveground biomass at the panicle initiation and heading stages. We also found that the POD, CAT, NR, and GOGAT of T2 for MX and YL at the heading stage were significantly enhanced compared to other treatments. Significant interaction was also observed in spikelet per panicle and 1000-grain weight between rice variety and nitrogen fertilizer type. Therefore, slow/controlled-release fertilizer application at the rate of 150 kg N per ha is a more feasible nitrogen fertilizer management strategy under mechanical deep placement, with the merit of increasing grain yield and improving nitrogen use efficiency in South China. Full article

Carpathian mountain grasslands are increasingly affected by management intensification and climatic variability, with consequences for species composition and ecosystem functioning. This study assessed the long-term effects of a mineral fertilization gradient and interannual climatic variability on indicator species dynamics and biomass production in a semi-natural high-nature-value (HNV) grassland in the Apuseni Mountains, based on a 17-year field experiment. Increasing fertilization intensity promoted a clear shift from species-rich oligotrophic communities toward simplified mesotrophic and eutrophic grassland types, accompanied by a decline in indicator species richness and the increasing dominance of competitive grasses. Biomass production increased consistently along the fertilization gradient. Climate-driven effects were assessed using unfertilized control plots, allowing management effects to be disentangled from interannual climatic variability. Variations in temperature and precipitation influenced floristic composition and productivity across the years, highlighting the sensitivity of mountain grasslands to short-term climatic fluctuations. Multivariate analyses revealed increasing vegetation homogenization under high fertilization and distinct year-to-year shifts in species composition under unfertilized conditions. These results emphasize the vulnerability of Carpathian HNV grasslands to both nutrient enrichment and climatic variability, and underline the need for climate-adaptive, biodiversity-oriented management strategies. Full article

Elevated atmospheric CO₂ is known to alter plant physiology, yet its specific effects on nutrient-rich leafy vegetables remain insufficiently quantified. This study aimed to examine how eCO₂ influences yield and nutritional quality in kale (Brassica oleracea) and spinach (Spinacia oleracea) through the first meta-analysis focused exclusively on these crops. Following the Collaboration for Environmental Evidence (CEE) guidelines, we systematically reviewed eligible studies and conducted a random-effects meta-analysis to evaluate overall and subgroup responses based on CO₂ concentration, crop type and exposure duration. Effect sizes were calculated using Hedges’ g with 95% confidence intervals. The analysis showed that eCO₂ significantly increased biomass in spinach (g = 1.21) and kale (g = 0.97). However, protein content declined in both crops (spinach: g = −0.76; kale: g = −0.61), and mineral concentrations, particularly calcium and magnesium, were reduced, with spinach exhibiting stronger nutrient losses overall. The variability in response across different CO₂ concentrations and exposure times further underscores the complexity of eCO₂ effects. These results highlight a trade-off between productivity and nutritional quality under future CO₂ conditions. Addressing this challenge will require strategies such as targeted breeding programmes, biofortification, precision agriculture and improved sustainable agricultural practices to maintain nutrient density. This research provides critical evidence for policymakers and scientists to design sustainable food systems that safeguard public health in a changing climate. Full article

The European chestnut (Castanea sativa Mill.) industry generates substantial amounts of underutilized biomass, including shells, leaves, and spiny burs. Distinguishing itself from existing literature, this review presents a novel, integrated life-science analysis that redefines these by-products as a complementary ‘bioactive triad’, ranging from metabolic regulators to anti-virulence agents, rather than interchangeable sources of polyphenols. Although traditionally discarded, these by-products are rich sources of polyphenols, ellagitannins, and flavonoids, with promising potential for nutraceutical, cosmetic, and pharmaceutical applications. This review examines recent advances in the valorization of chestnut by-products, focusing on extraction strategies, chemical profiles, and biological activities. Shell valorization has increasingly shifted toward green extraction technologies, such as subcritical water extraction and deep eutectic solvents, which strongly influence bioactive recovery and composition. Chestnut leaves emerge as a sustainable resource enriched in hydrolysable tannins with anti-inflammatory and quorum sensing-inhibitory properties, particularly relevant for dermatological applications. Spiny burs, often the most phenolic-rich fraction, display marked antioxidant activity and the ability to potentiate conventional antibiotics against pathogens such as Helicobacter pylori. Despite these promising features, major challenges remain, including cultivar-dependent chemical variability, the predominance of in vitro evidence, and safety concerns related to the accumulation of potentially toxic elements. Overall, while chestnut by-products represent valuable resources within circular bioeconomy frameworks, their successful industrial and practical translation will require standardized extraction protocols, robust bioavailability assessments, and well-designed in vivo and clinical studies to ensure safety and efficacy. Full article

Plasma-activated water (PAW) has gained attention across agricultural, medical, cosmetic, and sterilization fields due to its production of reactive oxygen and nitrogen species (ROS and RNS). Although PAW has been primarily explored for seed germination and sterilization in agriculture, its role as a nutrient source and physiological regulator remains less understood. In this study, PAW generated by a surface dielectric barrier discharge (SDBD) system contained approximately 1000 ppm nitrate (NO₃⁻) and was designated as PAW1000. Diluted PAW solutions were applied to sprout crops—wheat (Triticum aestivum), barley (Hordeum vulgare), radish (Raphanus sativus), and broccoli (Brassica oleracea var. italica)—grown under hydroponic and soil-based conditions. PAW100 and PAW200 treatments enhanced growth, increasing fresh biomass by up to 26%, shoot length by 22%, and root length by 18%, depending on the species. In silico analysis identified nitrogen-responsive transcripts among several autophagy-related genes. Consistent with this, fluorescence microscopy of Arabidopsis thaliana GFP-StATG8 lines revealed increased autophagosome formation following PAW treatment. The growth-promoting effect of PAW was diminished in atg4 mutants, indicating that autophagy contributes to plant responses to PAW-derived ROS and RNS. Together, these findings demonstrate that diluted PAW generated by SDBD enhances biomass accumulation in sprout crops, and that autophagy plays a regulatory role in mediating PAW-induced physiological responses. Full article

Background/Objectives: Stenotrophomonas maltophilia is an opportunistic pathogen causing severe infections, particularly in patients with cystic fibrosis (CF). Its intrinsic multidrug resistance and biofilm-forming capacity complicate treatment. Although biofilms are generally associated with antimicrobial tolerance, the relationship between biofilm formation and planktonic antibiotic resistance in S. maltophilia remains poorly understood. This study investigated the association between antibiotic resistance profiles and biofilm production in clinical isolates from CF and non-CF patients. Methods: A total of 86 clinical isolates (40 from CF airways and 46 from non-CF patients) were analyzed. Susceptibility to seven antibiotics was assessed by disk diffusion, and multidrug resistance profiles were defined using standard criteria. Biofilm formation was quantified after 24 h using a crystal violet microtiter plate assay and categorized by using a semiquantitative scale. Results: High resistance rates were observed, particularly to meropenem (87.2%), ciprofloxacin (80.2%), and rifampicin (72.1%). CF isolates exhibited significantly higher resistance to piperacillin/tazobactam and a greater prevalence of multidrug resistance. Biofilm formation was detected in 94.2% of isolates, with strong or powerful producers predominating. However, CF isolates formed significantly less biofilm than non-CF isolates. Notably, resistance to piperacillin/tazobactam and meropenem was associated with reduced biofilm biomass and a lower proportion of high biofilm producers. Across all isolates, an inverse correlation was observed between the number of antibiotic resistances and biofilm biomass. These trends persisted after stratification by clinical origin, although some comparisons did not reach statistical significance. Conclusions: This study reveals an unexpected inverse relationship between planktonic antibiotic resistance and biofilm-forming capacity in S. maltophilia. Enhanced biofilm production may represent an alternative persistence strategy in more antibiotic-susceptible strains, with important implications for infection management and therapeutic failure. Full article

Wood vinegar (WV), a by-product of woody biomass pyrolysis, is increasingly used in agriculture as a sustainable biostimulant, although its effects on plant stress resistance and underlying mechanisms remain poorly understood. Recent studies propose that WV may act through a eustress-based mechanism, defined as a mild and controlled stress that activates adaptive physiological responses and enhances plant performance without causing structural or metabolic damage. This study investigated the physiological and biochemical effects of WV on strawberry plants grown under three water-deficit stress levels [no stress (NS), moderate stress (MS), and high stress (HS)] and treated with WV either via fertigation (0.5% v/v, WV₁) or foliar spray (0.2% v/v, WV₂). Gas exchange parameters (A, g_sw, E, Ci, WUE), total chlorophyll content, and nutrient balance ratios (Fe/Mn and K/Ca) were measured after a three-month growth period. PERMANOVA revealed significant effects of both WV and water-deficit stress, as well as their interaction, on most parameters. Under NS and MS conditions, WV reduced A, gsw, E, and Ci while increasing WUE, indicating enhanced water-use efficiency and improved physiological adjustment to water limitation. Chlorophyll content remained stable, demonstrating preserved photosynthetic integrity. Nutrient ratios further supported a controlled ion rebalancing associated with adaptive stress responses under NS and MS, whereas HS conditions indicated the onset of distress. Overall, the data demonstrate that WV enhances plant stress resistance primarily by inducing eustress-mediated physiological regulation rather than by directly stimulating growth. Full article