Search Results (59)

The fungus Alternaria alternata, which causes tobacco brown spot disease, poses a serious threat to the tobacco industry. Beneficial microorganisms and their secondary metabolites have emerged as a promising green strategy for disease management. This study recovered 16 endophytic bacterial strains from Mentha haplocalyx Briq., a therapeutic herb. The study revealed that strain B5, with an inhibition rate of 82.76%, exhibited the highest antifungal activity against A. alternata. This strain exhibited broad-spectrum antifungal activity, with inhibition rates ranging from 66.34% to 87.23%. Phylogenetic analysis of 16S rDNA and gyrA gene sequences identified it as Bacillus velezensis (GenBank: PV168970 and PV173738). Further characterization revealed that strain B5 can secrete cell wall-degrading enzymes, produce IAA, and synthesize siderophores. The growth of mycelium in A. alternata was greatly reduced by both the ethyl acetate extract and the filtered liquid from the sterile fermentation, resulting in marked morphological abnormalities. Multiple antifungal active substances were identified through liquid LC-MS analysis. Greenhouse experiments demonstrated that the B5 fermentation broth effectively suppressed the occurrence of tobacco brown spot disease, achieving a relative control efficacy of 60.66%, comparable to that of 10% difenoconazole water dispersible granule (WDG). Additionally, strain B5 enhances plant disease resistance by activating the activities of key defense enzymes. B. velezensis B5 serves as a safe alternative to chemical fungicides and is highly effective at controlling tobacco brown spot disease. Full article

To ensure safe navigation, ship operators must not only meet the criteria defined in the International Maritime Organization (IMO) maneuverability standards but also understand maneuvering characteristics in restricted waters. This study numerically analyzed the hydrodynamic lateral forces and yaw moments acting on a stern trawler, a container ship, and a very large crude carrier (VLCC) with different hull forms as they navigated near a semi-circular bank wall. The effects of varying bank radius, lateral clearance, and water depth were examined. The results showed that the VLCC experienced the strongest attractive lateral force, while the stern trawler exhibited the most significant yaw moment. The hydrodynamic interaction patterns of the stern trawler and container ship were similar, whereas the VLCC displayed distinct behavior due to its fuller hull and greater inertia. These findings demonstrate that hull geometry significantly influences hydrodynamic interactions near boundaries, and the degree of response varies by ship type. The results provide valuable reference data for improving navigation safety in confined waters and preventing marine accidents such as collisions and groundings. This study contributes to a better understanding of ship–bank interaction and offers a theoretical basis for maneuvering assessments of various ship types in restricted maritime environments. Full article

Bacillus cereus poses a persistent challenge for human milk banks (HMBs) due to its ability to survive Holder pasteurization (HoP; 62.5 °C for 30 min). To ensure neonatal safety, any milk found to be contaminated post-HoP must be discarded, which impacts milk supply and adds to the operational demands of HMBs. In this study, we analyzed 688 B. cereus isolates from human milk (pre- and post-HoP), as well as from patient and environmental sources, to investigate human milk contamination by B. cereus at a Canadian HMB. Despite the limited temporal and geographic scope of the collection, the isolates exhibited remarkable genomic diversity, comparable to global B. cereus collections. Phylogenetic analysis at the core genome level revealed no clear clustering by isolate source, suggesting multifactorial pathways of B. cereus contamination. Isolates surviving HoP displayed gene variants linked to sporulation and cell wall integrity, suggesting a potential basis for HoP tolerance. Our findings emphasize that while genomic analyses offer major valuable insights, they alone are insufficient to address the complexities of B. cereus contamination in HMBs. Addressing this challenge will require combining genomic tools with robust monitoring systems, improved human milk-handling protocols, and pasteurization strategies better-suited to countering B. cereus resilience. Full article

A large number of pineapple (Ananas comosus) fruits are discarded in China every year due to softening. However, the underlying molecular mechanism is still unknown. AcoPG3 (GenBank accession number: XM020243935), a pineapple gene of polygalacturonase, was found to be the major gene responsible for the softening of pineapple fruit. Fruit of AcoPG3-overexpressing tomato (Solanum lycopersicum var. Jingfan 101) begins to soften 9 days earlier than that transformed with a net vector. Fruit of AcoPG3-overexpressing pineapple (APG3-2) begins to soften 6 days earlier than that transformed with a net vector. Fruit of MPG3-1, a pineapple line in which AcoPG3 is mutated, begins to soften 31 days later than that transformed with a net vector. The sequence of polygalacturonase activities in fruit from the highest to the lowest was APG3-2, wild type, MPG3-1. The same sequences were also found in the liquid content of apoplast and the electrolyte leakage of pineapple pulp. The order of methyl-esterified pectin content in the pulp cell wall, from the highest to the lowest, was MPG3-1, wild type, and APG3-2. The same order was also observed for the contents of non-methyl-esterified homogalacturonan and rhamnogalacturonan-I in the pulp cell wall. The AcoPG3 mutation resulted in a decrease in polygalacturonase activity in pineapple fruit, decreasing the degradation of methyl-esterified pectin, non-methyl-esterified homogalacturonan, galactan and rhamnogalacturonan-I in the pulp cell wall. Fruit softening can be deferred, and the shelf life can be extended by mutating the AcoPG3 gene. Full article

The influence of various factors, such as the natural properties of rock and in-situ stress conditions, results in uneven and rough fracture surfaces post-hydraulic fracturing. This significantly impacts the transport and placement of proppant within the fracture, thereby affecting the effectiveness of fracture stimulation. This study employs the rectangular wave method to characterize the roughness of fracture wall morphology, detailing the variation of roughness by altering the number and height of micro-protuberances, and constructs a three-dimensional model of rough fractures. The Euler–Euler model is utilized to simulate the placement and transport patterns of proppant within the fracture. Sand banks within the fracture profile are segmented based on proppant concentration, and the dimensionless area of each concentration interval is calculated to analyze the structure of sand banks and the suspension and settling effects of proppant. This research investigates the variation patterns of sand dune structures within fractures characterized by different levels of roughness and morphologies; it also examines the impact of injection velocity and fracturing fluid viscosity on the transport and placement of proppant within rough fractures. The findings indicate that the complex spatial structure of rough fractures modifies the edge shape of sand dunes. Moreover, it impedes proppant transport, leading to the formation of sand plugs near the wellbore. The maximum distance of sand placement for rough fractures is only 55.2% of that for fractures without considering roughness. The increase in the number and height of micro-protrusions enhances fracture roughness, leading to a stronger retarding effect. However, variations in these two types of roughness have distinct impacts on the morphology of sand dunes. Higher injection velocities facilitate the transport of proppant within rough fractures. The furthest distance of proppant placement at an injection velocity of 0.5 m³/min is only 68.4% of that at an injection velocity of 1.5 m³/min. The study’s findings contribute to a more intuitive understanding of the impact of rough fracture wall surfaces on the transport and placement patterns of proppant, providing a foundation for the optimization of fracturing design and operational parameters. Full article

High-frequency induction welding technology represents the development direction of the high-temperature corrosion protection technology for the heating surfaces of the boiler “four tubes”. However, when the high-frequency induction coil heats and remelts the coating on the tube’s outer wall, the tube’s inner wall is also heated, causing an iron oxide scale to form on the tube’s inner wall. When the remelting temperature rises and the temperature of the tube’s inner wall exceeds 580 °C, three layers of oxide films, FeO, Fe₃O₄, and Fe₂O₃ are arranged in sequence from the substrate surface of the tube’s inner wall to the outside, with a thickness ratio of approximately 1:10:100. From the XRD spectra of tube iron oxide scale, it can be seen that the oxidation of the tube. The skin is mainly composed of Fe₃O₄, with a certain amount of Fe₂O₃ and trace amounts of FeO. The iron in the diffraction peak originates from the metal matrix. However, when the remelting temperature continues to rise and the temperature of the tube’s inner wall exceeds 580 °C, the oxide film begins to thicken significantly, that is, the oxide film begins to transform into an oxide scale. Under the continuous action of high-temperature induction remelting, the reaction between iron and oxygen is accelerated, but because the oxygen ions of water slowly diffuse through two outer layers of oxide films, with a low oxygen concentration. Although the FeO film is thin, it has a loose structure and numerous lattice defects, is unstable and easy to decompose, and easily peels off from the tube’s inner wall. For a pipe wall thickness of 5 mm, if the thinning rate of the inner wall caused by detachment reaches 0.8 mm/year, it is highly likely to cause pipe burst accidents within 4–5 years. The influence of the iron oxide scale on the performance of the tube’s inner wall was evaluated by testing indexes, such as surface hardness and decarburization layer depth. Although the oxide scale reduces the surface hardness of the tube’s inner wall, the surface decarburization layer is very thin, so the effect on the mechanical properties of the tube’s substrate is limited. The technology of inhibiting the formation of the iron oxide scale in induction remelting is briefly introduced. During the high-frequency remelting process of water-cooled walls, as the tube bank moves forward relative to the high-frequency heating coil, nitrogen protection is used to suppress the formation of oxide scale, effectively eliminating the troubles caused by high-frequency induction remelting and achieving the goal of improving the service life of the tube bank. This technology of the nitrogen protection method is used to inhibit the formation of iron oxide scale, not only inhibiting the formation of the iron oxide scale on the tube inner wall and the back of the tube bundle, with remarkable experimental results and broad application prospects. Full article

Urban inland rivers are closely related to urban development, but high-density urbanisation has reduced the natural function of streams and the riverbanks are hardened into two parts, embankment walls and berms, which give rise to a variety of riparian landscapes. However, the difference in the height of riparian walkways affects the degree of their greening and landscape effects. In this paper, we studied single- and double-decker urban greenways, constructed quantitative indicators of spatial elements based on deep learning algorithms using an image semantic segmentation (ISS) model that simulates human visual perception, used random forests and multivariate linear regression models to study the impact of the height difference of the linear riverfront greenway on visual perception, clarified the impact of the visual landscape differences caused by different types of space on landscape aesthetic preferences (LP) and confirmed the impact of the specific extent to which landscape components influence preferences. The results of the study showed that there were significant differences in landscape perception scores between the single and double layers. (1) The influence of WED (negative correlation) and NI (positive correlation) is large in the single-layer greenway. The colour, material and structure of the guardrail can be beautified and diversified and the quality of the greenery can be taken into account to maintain the visibility of the greenery in order to improve the score of the single-layer greenway. (2) The significant influence of BVI in the double-layered greenway is positive. Water-friendly or water-viewing spaces can be added appropriately to improve the landscape score of double-layered greenways. This study is applicable to the regional landscape feature identification of single- and double-decker greenways on large-scale urban hard barge bank images, which realises the whole-region feature identification of a large-scale human perspective and is an effective expansion of analysis techniques for sustainable landscape planning and the design of riparian greenways. Full article

Ustilago esculenta is a dimorphic fungus that specifically infects Zizania latifolia, causing stem swelling and the formation of an edible fleshy stem known as jiaobai. The pathogenicity of U. esculenta is closely associated with the development of jiaobai and phenotypic differentiation. Msb2 acts as a key upstream sensor in the MAPK (mitogen-activated protein kinase) signaling pathway, playing critical roles in fungal hyphal growth, osmotic regulation, maintenance of cell wall integrity, temperature adaptation, and pathogenicity. In this study, we cloned the UeMsb2 gene from U. esculenta (GenBank No. MW768949). The open reading frame of UeMsb2 is 3015 bp in length, lacks introns, encodes a 1004-amino-acid protein with a conserved serine-rich domain, and is localized to the vacuole. Expression analysis revealed that UeMsb2 is inducibly expressed during both hyphal growth and infection processes. Deletion of UeMsb2 did not affect haploid morphology or growth rate in vitro but significantly impaired the strain’s mating ability, suppressed filamentous growth, slowed host infection progression, and downregulated the expression of b signaling pathway genes associated with pathogenicity. Notably, the deletion of UeMsb2 did not influence the in vitro growth of U. esculenta under hyperosmotic, thermal, or oxidative stress conditions. These findings underscore the critical role of UeMsb2 in regulating the pathogenicity of U. esculenta. This study provides insights into the interaction between U. esculenta and Z. latifolia, particularly the mechanisms that drive host stem swelling. Full article

Ancient monumental complexes need continuous analysis and monitoring operations to preserve a good conservation status. For this reason, the analysis of decay and alteration phenomena represents one of the main activities for their preservation. At the same time, the diffusion of Heritage Building Information Modelling (HBIM) methodology opens new scenarios for the management of Architectural Heritage. The paper describes the workflow based on a Scan-to-BIM approach for the generation of a decay map in an HBIM model. The workflow was applied to a significant case study, the church of “Santa Maria della Grotta” in Marsala (Italy). This church, partially excavated in a sandstone bank, is part of a larger heritage site consisting of a series of hypogea and a Punic necropolis dating back more than a thousand years. The Scan-to-BIM process, relying on an integrated survey combining mobile laser scanning and photogrammetric technologies, enabled the achievement of a complete 3D parametric model of the monument and, altogether, a detailed decay map in a BIM environment. The mapping process focused on the production of thematic maps of perimetral walls according to an abacus of decays implemented in a BIM system, useful for the analysis and conservation of the church. The work demonstrates how the Scan-to-BIM process is an efficient approach for 3D data collection and how it could facilitate the identification and mapping of pathogenic phenomena. Furthermore, the inclusion of this kind of information in the BIM model represents an effective tool for the maintenance and restoration of built heritage. Full article

This study presents a comprehensive palaeoenvironmental reconstruction of the southern part of the Danube–Tisza interfluve in the Carpathian Basin from the Late Pleistocene to Early Holocene, addressing the region’s response to global climate forcings and local environmental factors based on multi-proxy analyses of two key protected areas: the Katymár brickyard and the hay meadows and loess banks of Hajós. By integrating radiocarbon-dated malacological, macrobotanical, pollen, phytolith, geochemical, and sedimentological analyses, it was possible to provide a picture of past climate–ecosystem interactions. The Katymár North sequence provides an extended chronology from Marine Isotope Stage 3 (MIS3, ca. 36,000 cal BP) through the Last Glacial Maximum (LGM) and into the Early Holocene, while the Hajós sequence offers high-resolution data for the LGM–Holocene transition. By the late Ice Age, humidity and surface moisture superseded temperature as primary palaeoecological limiting factors, promoting ecotone-like forest–steppe environments during cooling periods. Full article

This study presents a comprehensive bibliometric analysis of research on bank erosion and control measures, utilizing the Scopus database and VOSviewer software. Key terms such as “bank”, “erosion”, “control”, and “protection” frequently appear in the literature, underscoring their importance in studies on riverbank erosion. Since 2000, scientific production has steadily increased, particularly in disciplines such as Environmental Sciences and Earth and Planetary Sciences, driven by growing concerns about climate change and sustainable water resource management. Countries with substantial research resources, such as the United States and China, lead in the production of studies, reflecting their commitment to addressing this global issue. In parallel, the evaluation of erosion mitigation practices in Colombia revealed that, although effective techniques such as gabion walls and riparian vegetation exist, 40% of respondents do not implement specific measures. This lack of implementation is attributed to insufficient knowledge, limited resources, and misconceptions about the effectiveness of these techniques. The findings highlight the need to promote proven practices and enhance professional training. Future research should focus on developing more accurate predictive models, integrating interdisciplinary approaches, and assessing the impacts of climate change on bank erosion. Addressing barriers to applying effective techniques at the local level and improving access to resources and knowledge are critical steps to reducing bank erosion and ensuring sustainable water management. Full article

Background: Clonidine has been used in clinical medicine, e.g., to treat high blood pressure and other conditions. Animal studies have linked its use to impairments of male reproductive functions, and although only a few reports exist for the human species, such actions may exist in man as well. The underlying reasons and, specifically, possible actions of clonidine at the level of the testis are not known. Introduction: Clonidine is an agonist at the α_2A-adrenoceptor (ADRA2A), which, as data bank mining indicated, is expressed by several cells of the human testis. The human testis and most of its cells are, however, not readily accessible to experimental testing. Cells from the peritubular wall compartment (human testicular peritubular cells; HTPCs) are the exception. Methods and Results: As shown by immunohistochemical/immunocytochemical and PCR techniques these cells express ADRA2A and retain expression upon isolation and culture. When tested over a concentration range (1–1000 µM) and 24 h, clonidine did not visibly affect HTPC morphology but significantly stimulated IL6 mRNA levels in a concentration-dependent manner. ELISA measurements of cell culture supernatants confirmed a stimulatory action of clonidine (10 µM) on secreted IL6. When examined in collagen gel contraction assays of HTPCs, clonidine (10 µM) exerted a slight relaxing action, while a proteomic study revealed that clonidine (10 µM) did not significantly change cellular protein abundance of HTPCs after 24 h (data available via ProteomeXchange with identifier PXD052220). Conclusion: Thus, ADRA2A-bearing cells in the human testis are targets for catecholamines and drugs such as clonidine. The results of this HTPCs-focused study only show the tip of the iceberg. It is likely that catecholamines/catecholaminergic drugs have the potential to interfere with human testicular functions. Full article

The present study evaluates the phytotoxic effects of phloretin, a prevalent secondary metabolite of apple trees, on the broadleaf weed Capsella bursa-pastoris (L.) Medik. known for its resistant myxospermous seeds that form a long-lasting soil bank. The results indicate a significant, dose-dependent inhibitory effect of phloretin on the growth and morphological parameters of weed seedlings grown in vitro. Although the applied phloretin concentrations (250–1000 µM) were not lethal to the C. bursa-pastoris seedlings after two weeks, the metabolism of the seedlings was impaired, resulting in an accumulation of lipid droplets in the root tips and root hairs. Histochemical analysis shows deposits of phenols in the root epidermal cells, which are probably aggregates of phloretin or its metabolic derivatives. The accumulation of pectin in the cell walls of root border cells in phloretin-treated seedlings indicates an attempt to reduce the uptake of phloretin and reduce its concentration in the cells. Inhibition of shoot growth associated with chlorosis and reduced photosynthetic pigment content is a consequence of seedling exposure to phloretin. This study provides a basis for further evaluation of phloretin as a new bioherbicidal compound and for elucidating the mechanism underlying its phytotoxic activity. Full article

The Great Wall is a world-renowned cultural heritage site and a national key protected cultural relic in China. The plants on the top surface of the Great Wall heritage site are one of the factors leading to the occurrence and continuous deterioration of the Great Wall. Therefore, there is ongoing academic controversy on how to scientifically dispose of the plants on the top surface of the Great Wall heritage site. Taking Beijing Dazhuangke Great Wall as an example, the preliminary field research was completed. This paper further describes the key technology for the disposal of remaining roots after removing the ground part of the plants from the top surface of the Great Wall heritage site and clarifies the basic idea of using the original covered soil on the top surface of the Great Wall heritage site, back-filling the original covered soil stripped via reparation of the layers and the regenerated plants in a seed bank to create ‘soft capping’ protection. This study provides a basic framework for ‘soft capping’ on the top surface of the Great Wall heritage site and construction of the cultural landscape of the ‘Garden on the Great Wall’. Full article

This study proposes a new empirical model for estimating local scour along the base of longitudinal training walls for granular riverbeds. The model’s performance was rigorously assessed through experiments conducted in an open-channel flume, encompassing variations in granulometric characteristics, slope, and flow rates. The investigation involved a comparative analysis of six commonly employed equations for scour estimation. The results consistently demonstrated a tendency of the selected equations to overestimate scour depth within the longitudinal structures. In contrast, the new proposed equation considers factors such as the well-graded granular bedding represented by the Coefficient of uniformity (C_u) and the embedment of the longitudinal wall. This allows for a more robust identification of the scour behavior of longitudinal walls. This research enhances our comprehension of local scour in riverbeds. It provides engineers and researchers with a valuable tool for more accurate predictions, thereby contributing to the improved design and maintenance of river environment structures. Full article