Search Results (158)

Bee pollen is a natural nutrient substance collected by bees from plants. Its metabolites have been extensively studied, yet the characteristic metabolites of bee pollen from different floral sources have not been clearly identified. In this study, we collected four types of bee pollen (tea, rose, rapeseed, and corn pollen) from across China and analyzed their volatile and non-volatile metabolites using liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and gas chromatography-mass spectrometry (GC-MS). At the same time, the nutritional substances (Including polyphenols, organic acids, and sugars) were precisely quantified. The results showed that the total phenols (5 mg GAE/g) and total flavonoids (0.27 mg RE/g) content of corn pollen were significantly higher (p < 0.05) than those of other pollens, and the contents of polyphenols such as naringenin were relatively high, indicating strong antioxidant potential. Rose pollen was rich in protein (0.04 g/g) and flavonoid glycosides. Tea pollen was prominent in the content of polyphenol glycosides and amino acid derivatives, while rapeseed pollen performed well in phenolic acids (Ferulic acid), as well as specific sugar (Mannose). We identified the differential metabolites of these bee pollen through orthogonal partial least squares discriminant analysis (OPLS-DA) (VIP > 1). It was also stipulated that metabolites with a VIP value greater than 1.5 showed significant differences and could be used as characteristic metabolites for differentiating pollen (p < 0.05). The representative metabolites of bee pollen were as follows: rapeseed pollen—ferulic acid; tea pollen—malic acid; corn pollen—epicatechin; and rose pollen—fumaric acid. This study provides a research basis for evaluating the quality, traceability, and metabolite exploration of bee pollen. Full article

Partial or total replacement of pork fat with homologous functional oils may meet consumer demand for healthy meat products while preserving their sensory quality. This study investigated the use of lard-derived diacylglycerol (DG) as a fat replacer on the flavor characteristics of frankfurters. The results revealed that substituting pork fat with purified glycerolized lard (PGL) at different levels (25%, 50%, and 100%) increased the water content and water activity, improved the L* and b* values, and protein thermal stability, while decreasing the a* value of frankfurters. Meanwhile, electronic nose results showed that replacing pork fat with PGL affected the aroma of frankfurters. Furthermore, gas chromatography–mass spectrometry detected 50 volatile compounds in all the frankfurters (such as aldehydes, alcohols, terpenes, and aromatic hydrocarbons, etc.). Replacing lard with PGL significantly increased the variety and content of flavor compounds in frankfurters (p < 0.05). According to the approximate odor activity values (OAV) > 1 and variable importance in projection (VIP) > 1, the distinct flavor of the frankfurters with different levels of PGL mainly resulted from aldehydes, alcohols, and terpenes. Generally, this study provided a valuable theoretical foundation for substituting fat with lard-derived DG to improve the flavor characteristics of frankfurters. Full article

Drought responses in poplar are genotype-dependent, yet standardized metrics for quantifying drought tolerance remain scarce. Here, we employed logistic modeling of relative electrolyte leakage (REC) for the first time in poplar to derive clone-specific semi-lethal polyethylene glycol (PEG) concentrations (LC₅₀), transforming a traditional descriptive assay into a quantitative, high-throughput drought-injury metric. Six elite Populus cultivars were exposed to increasing PEG concentrations, and their REC curves were fitted using a logistic function (R² = 0.885−0.981). The derived semi-lethal PEG concentration (LC₅₀) ranged from 7.99% in ‘PZ1’ (drought-sensitive, SS) to 13.44% in ‘YX2’ (drought-tolerant, ST), enabling clear classification. Under 10% PEG stress, ST maintained leaf water content (LWC) at 73%, while SS dropped to 63%. Malondialdehyde (MDA) content doubled in SS (44.7 nmol·g⁻¹ FW) but increased by only 25% in ST (33.5 nmol·g⁻¹ FW). Gas exchange analysis revealed that SS exhibited approximately twice the reduction in net photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (Gs) compared to ST, with intercellular CO₂ accumulation (Ci) occurring only in SS—indicating both stomatal and non-stomatal limitations. Osmolyte profiling showed that SS accumulated large amounts of soluble sugars (Ss) (+128%) and proline (Pro) (+230%), whereas ST maintained stable soluble protein (Sp) levels and only moderately increased proline (+120%). Antioxidant capacity differed markedly: catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activities increased by 5.6-, 1.8-, and 2.0-fold in ST, respectively, compared to 3.4-, 1.3-, and 1.7-fold in SS. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) of ten physiological traits explained 89% of the total variance (R²X = 0.954, Q² = 0.973), identifying POD, SOD, CAT, and Pro as the most discriminative variables (VIP > 1). This four-marker signature converts a conventional dose–response assay into a rapid, low-cost screening module that can be deployed in robotic phenotyping platforms. Specifically, the high-ranking genotype ‘YX2’ is recommended for immediate use in water-limited plantations and as a robust parent for next-generation dryland breeding programs. Full article

Objective: To systematically evaluate the efficacy and explore the mechanisms of Yiqi and blood-activating traditional Chinese medicine (TCM) combined with chemotherapy in treating non-small cell lung cancer (NSCLC). Methods: Randomized controlled trials (RCTs) on chemotherapy combined with Yiqi and blood-activating TCM for NSCLC were retrieved from CNKI, VIP, Wanfang, and PubMed databases. The search period covered from the inception of each database to January 2025. Study quality was assessed via the Cochrane Risk of Bias tool. Meta-analysis evaluated clinical efficacy, data mining identified core herbs and active compounds, and network pharmacology analyzed targets and pathways. Immune infiltration analysis explored immunomodulatory mechanisms. Results: A total of 57 RCTs with 4865 patients were analyzed. Combined therapy significantly improved short-term efficacy, relieved symptoms (e.g., cough, fatigue), reduced adverse effects, and enhanced quality of life versus chemotherapy alone. Data mining identified Astragalus membranaceus, Atractylodes macrocephala, and Poria cocos as core herbs. Network pharmacology revealed 40 active compounds (including quercetin and kaempferol) that targeted 137 key proteins (e.g., TP53, AKT1), with these targets mainly involved in immune regulation. Immune infiltration analysis showed increased CD4+ T cells and balanced T cell subsets, indicating enhanced antitumor immunity. Conclusions: Yiqi and blood-activating TCM combined with chemotherapy improves NSCLC outcomes by modulating the tumor immune microenvironment. This supports the integration of TCM and highlights immune regulation as a key mechanism. Full article

Introduction: The ability of salt-processed Alismatis Rhizoma (SAR) (Alisma plantago-aquqtica L.) to nourish Yin and promote urination is stronger than that of Alismatis Rhizoma (AR). However, there are few studies focused on evaluating the quality of its medicinal materials. Objectives: This study aimed to identify potential quality markers (Q-markers) for SAR, thereby providing a more reliable basis for its quality control and clinical application. Methods: Q-markers were identified through fingerprinting and chemical pattern recognition analysis of 15 batches of SAR. The diuretic effects of these markers were then verified by network analysis and molecular docking. Results: HPLC fingerprints of 15 SAR batches were established, with similarity analysis showing values > 0.85 (0.852–0.990). Chemical pattern recognition identified six critical compounds contributing to SAR quality: alisol F, alisol C 23-acetate, alisol A, alisol A 24-acetate, alisol B 23-acetate, and an alisol O isomer (VIP > 1.0). Network analysis revealed 76 overlapping targets between these compounds and diuretic-related diseases, with core targets including non-receptor tyrosine kinase (SRC), epidermal growth factor receptor (EGFR), mitogen-activated protein kinase 1 (MAPK1), which were identified through protein–protein interaction (PPI) network analysis, with degrees of 27, 24, and 22, respectively. Key pathways involved were the EGFR tyrosine kinase inhibitor resistance pathway, calcium signaling pathway, tumor necrosis factor signaling pathway, etc. Molecular docking confirmed strong binding interactions between the Q-markers and the hub targets, particularly alisol B 23-acetate with MAPK1 (−60.10 kcal·mol⁻¹) and alisol A 24-acetate with EGFR (−46.14 kcal·mol⁻¹) and SRC (−48.86 kcal·mol⁻¹). The diuretic effects of SAR are likely mediated through anti-inflammatory actions and regulation of water–sodium balance via multi-target and multi-pathway mechanisms. Conclusion: This study provides a robust foundation for quality control and clinical application of SAR, though further in vivo validation is warranted. Full article

Background/Objectives: The aim of this study was to explore the effects of diets with different protein levels on the metabolite composition and metabolic pathways of the longest dorsal muscle of Tibetan pigs, in order to provide a metabolic basis for optimizing the nutritional regulation strategy of Tibetan pigs. Methods: A total of 32 healthy 180-day-old depopulated male Tibetan pigs were randomly divided into four groups and fed diets with protein levels of 10%, 12%, 14%, and 16%, respectively, with a feeding cycle of 8 weeks. The longest dorsal muscle samples were collected, and their metabolic profiles were systematically analyzed by LC-MS non-targeted metabolomics. Results: The TIC plots of the quality control samples were highly overlapped, indicating a stable instrumental detection process and good consistency of sample processing. Principal component analysis and orthogonal partial least squares discriminant analysis revealed significant metabolic differences between groups with different protein levels. A total of multiple differential metabolites was obtained based on VIP value and p-value screening, and Venn diagram analysis revealed a total of 11 metabolites among the three comparative groups, suggesting that they may have key roles in the protein regulation process. Volcano plots further clarified the number and trend of significantly up- and down-regulated metabolites in each group. KEGG pathway enrichment analysis showed that, with the elevation of protein level, the metabolic pathway response showed a tendency of expanding from basal energy metabolism to the complex network of amino acid synthesis, steroidogenesis, endocrine signaling, and detoxification pathways, especially in the high-protein-treated group. Conclusions: The study showed that different protein intake levels could significantly regulate the metabolites and key metabolic pathways in the longest muscle of Tibetan pigs, which provided theoretical support for the scientific formulation of a protein supply program to optimize the quality and growth performance of Tibetan pork. Full article

The vegetative insecticidal protein Vip3Aa from Bacillus thuringiensis (Bt) has become a key plant-incorporated protectant (PIP) in transgenic crops targeting lepidopteran pests, particularly as resistance increasingly compromises the efficacy of Cry protein PIPs. More than a decade after its commercial deployment, Vip3Aa performance remains efficacious but increasingly vulnerable. Field screens have detected unexpectedly high baseline frequencies of Vip3Aa-resistant alleles and have produced highly resistant strains in several major pests, including Helicoverpa spp., Spodoptera spp., and Mythimna separata. Although structure–function experiments and studies on resistance to Vip3Aa have identified altered midgut processing and impaired receptor binding as candidate resistance mechanisms, the underlying genetic determinants remain poorly understood. Moreover, resistance to Vip3Aa appears to diverge from canonical Cry protein resistance pathways, underscoring the need for dedicated mechanistic studies. This review critically examines the available experimental evidence on Vip3Aa resistance mechanisms, highlighting major knowledge gaps and proposing research priorities to inform resistance monitoring and extend the durability of Vip3Aa-based pest control. Full article

As demand for plant-based beverages grows, analytical tools are needed to classify and understand their structural and compositional diversity. This study applied a multi-analytical approach to characterize 41 commercial almond-, oat-, rice- and soy-based beverages, evaluating attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, protein secondary structure proportions, colorimetry, and microscopic image texture analysis. A total of 26 variables, derived from ATR-FTIR and protein secondary structure assessment, were employed in multivariate models, using partial least squares discriminant analysis (PLS-DA) and orthogonal PLS-DA (OPLS-DA) to evaluate classification performance. The results indicated clear group separation, with soy and rice beverages forming distinct clusters while almond and oat samples showing partial overlap. Variable importance in projection (VIP) scores revealed that β-turn and α-helix protein structures, along with carbohydrate-associated spectral bands, were the key features for beverages’ classification. Textural features derived from microscopy images correlated with sugar and carbohydrate content and color parameters were also employed to describe beverages’ differences related to sugar content and visual appearance in terms of homogeneity. These findings demonstrate that combining ATR-FTIR spectral data with protein secondary structure data enables the effective classification of plant-based beverages, while microscopic image textural and color parameters offer additional extended product characterization. Full article

: Negative energy balance (NEB) in dairy cows induces excessive lipolysis, leading to elevated levels of β-hydroxybutyric acid (BHBA), which, when accumulated, can cause liver damage. Rutin (RT), a natural flavonoid with antioxidant and anti-inflammatory properties, has demonstrated potential hepatoprotective effects; however, its ability to mitigate BHBA-induced hepatocellular injury in calves remains unclear. This study first assessed the impact of various BHBA concentrations on oxidative stress in calf hepatocytes, then explored the protective effects and underlying mechanisms of RT, and finally employed untargeted metabolomics to further elucidate RT’s mode of action. The results showed that exposure to 1.2 mM BHBA significantly increased malondialdehyde (MDA), nitric oxide (NO) contents, and reactive oxygen species (ROS) levels, while markedly decreasing glutathione (GSH) content and catalase (CAT) activity compared with the blank control. Notably, pretreatment with 100 μg/mL RT resulted in the greatest increase in GSH contents (180%) compared to BHBA treatment alone, while 150 μg/mL RT led to the most pronounced reduction in MDA contents (220%). Furthermore, BHBA treatment significantly upregulated the expression of Kelch-like ECH-associated protein 1 (Keap1) and downregulated nuclear factor erythroid 2-related factor 2 (Nrf2), NAD(P)H quinone dehydrogenase 1 (NQO1), and heme oxygenase-1 (HO-1) at both the mRNA and protein levels. These alterations were effectively reversed by pretreatment with 100 μg/mL RT. Non-targeted metabolomics identified 1525 metabolites in total. Based on OPLS-DA, metabolites with a variable importance in projection (VIP) > 1 and p < 0.05 were considered significantly altered. Compared with the blank control, BHBA treatment upregulated 47 metabolites—including 8-hydroxy-2′-deoxyguanosine, 3-hydroxyisovaleric acid, and N-palmitoyl-sphingosine—and downregulated 58 metabolites, such as betaine, linolenic acid, and arachidonic acid. In contrast, RT pretreatment upregulated 207 metabolites relative to the BHBA treatment, including linolenic acid, taurocholic acid, and 4-hydroxybenzoic acid, and downregulated 126 metabolites, including 3-hydroxyisovaleric acid, 8-hydroxy-2′-deoxyguanosine, and pyruvaldehyde. Pathway enrichment analysis indicated that RT alleviated BHBA-induced hepatocyte injury primarily by modulating the fatty acid degradation pathway. In summary, RT mitigated BHBA-induced oxidative stress in calf hepatocytes by regulating the Keap1/Nrf2 signaling pathway and further exerted protective effects through metabolic reprogramming. Full article

Microglial cells, the resident immune cells of the central nervous system (CNS), are essential for maintaining CNS homeostasis. Dysregulation of microglial function is implicated in the pathogenesis of various neurodegenerative diseases. Vasoactive intestinal polypeptide receptors 1 and 2 (VPAC1 and VPAC2) are G-protein-coupled receptors (GPCRs) expressed by microglia, with their primary ligands being pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP). However, the specific roles of VPAC-type receptors in microglial regulation remain poorly understood. In this study, we generated VPAC1^+/− and VPAC2^+/− BV2 microglial cell lines using CRISPR-Cas9 gene editing and conducted a series of biological and molecular assays to elucidate the functions of these receptors. Our findings demonstrated that both mutant cell lines exhibited a polarized phenotype and increased migratory activity. VPAC1^+/− cells showed enhanced survivability and baseline activation of the unfolded protein response (UPR), a protective mechanism triggered by endoplasmic reticulum (ER) stress, whereas this response appeared impaired in VPAC2^+/− cells. In contrast, under lipopolysaccharide (LPS)-induced inflammatory conditions, UPR activation was impaired in VPAC1^+/− cells but restored in VPAC2^+/− cells, resulting in improved survival of VPAC2^+/− cells, whereas VPAC1^+/− cells exhibited reduced resilience. Overall, our findings suggest that VPAC1 and VPAC2 receptors play distinct yet complementary roles in BV2 microglia. VPAC2 is critical for regulating survival, ER stress responses, and polarization under basal conditions, while VPAC1 is essential for adaptive responses to inflammatory stimuli such as LPS. These insights advance our understanding of microglial receptor signaling and may inform therapeutic strategies targeting microglial dysfunction in neurodegenerative diseases. Full article

(1) Backgroud Astragalus, a traditional Chinese medicine, demonstrates therapeutic effectiveness in acute pancreatitis (AP). Nevertheless, its precise pharmacological mechanism remains unclear, and clinical guidelines have not been established. This study aims to systematically elucidate the active compounds and molecular mechanisms underlying Astragalus’ therapeutic effects in AP, and provide clinical evidence supporting its efficacy. (2) Methods: TCMSP and Swiss Target Prediction identified drug targets; GeneCards, DrugBank, and OMIM provided disease targets. Venny determined the therapeutic targets, while STRING constructed a protein–protein interaction network. Cytoscape 3.10.3 validated core targets. DAVID was used to conduct GO and KEGG pathway analyses, visualized via Bioinformatic platform. Cytoscape 3.10.3 was used to build a “drug–ingredients–targets–pathways–disease” network. AutoDock Vina 1.1.2 and AutoDockTools 1.5.7 was used to performed molecular docking, with PyMOL 3.0 visualizing the results. PubMed, Embase, Cochrane, Web of Science, CNKI, Wanfang, VIP, and CBMdisc were searched. The literature was screened, extracted, and evaluated, followed by a meta-analysis, using RevMan 5.4.1 and Stata 18. (3) Results: We identified 539 targets for the active ingredients of astragalus. Among 1974 disease-related targets, 232 were found to be therapeutic targets. The GO analysis yielded 589 entries, while the KEGG pathway enrichment analysis identified 147 relevant pathways. The top five active ingredients were quercetin, kaempferol, isorhamnetin, formononetin, and calycosin. Molecular docking analysis revealed potential synergistic effects between these components and core targets. The meta-analysis, comprising six randomized controlled trials, demonstrated a significantly higher total effective rate of clinical efficacy in the astragalus group compared to the control group. (4) Conclusions: Astragalus treats AP through the synergistic action of its components, targets, and pathways. Key active compounds, such as quercetin, kaempferol, isorhamnetin, formononetin, and calycosin, engage with pivotal targets, including TP53, AKT1, TNF, IL6, EGFR, CASP3, MYC, and HIF1A, within primary pathways, such as pathways in cancer, PI3K-Akt signaling pathway, and lipid metabolism, and atherosclerosis. Astragalus effectively treats AP and alleviates clinical symptoms by reducing the time for gas or defecation passage, the disappearance time of abdominal pain or distension, and the recovery time of bowel sounds. Full article

Background: Nutritional biomarkers are essential for assessing the impact of dietary interventions on health. Amino Acids (AA) serve as potential biomarkers due to their role in metabolic pathways, although the influence of combining macronutrients on AA metabolism remains unclear. Objective: This study aims to evaluate the effects of combining macronutrients (fat, protein, and fiber) on AA metabolism. Methods: A dietary intervention was conducted with 41 participants who were assigned to two groups: the Protein Fat (PF) group and the Protein Fat and Fiber (PFF) group. AA concentration was measured using LC-MS/MS. Results: Our findings indicated that combining macronutrients reduced plasma levels of AA with statistically significant differences between the two diets (p < 0.05 for EAA and BCAA; p < 0,01 for NEAA and AAA). Metabolic profile analysis showed differences between the groups, especially at 55 days (55 D) (R2X: 0.749; R2Y: 0.589; Q2: 0.582, p < 0.001) and 98 days (98 D) (R2X: 0.886; R2Y: 0.7; Q2: 0.605, p < 0.001). Five metabolites (leucine, asparagine, alanine, cysteine, and creatine) were the most influential factors in group differentiation (VIP score), with values ranging between 1.04 and 1.75. Conclusions: The combination of macronutrients led to a significant reduction in plasma amino acids and amines in the PFF group, with recovery post-intervention. These findings highlight the possibility that these metabolites are related to different health statuses. Full article

In this study, the impacts of water-bath reheating (WR), steam reheating (SR), air-frying reheating (AR), roasting reheating (RR), and microwave reheating (MR) on the physicochemical properties, oxidation, and flavor profiles of crispy chicken (CC) were investigated. The results revealed that the pH of CC was significantly reduced after reheating (p < 0.05). The AR samples had a slight change in L* and the highest springiness. The RR samples had the highest degree of lipid and protein oxidation. In addition, WR, AR, RR, and MR treatments effectively increased the contents of umami-related amino acids. Glu and Cys were typically the taste-active amino acids in CC. AR contributed to increasing the response values of umami and richness. As shown by the electronic nose and Gas Chromatography–Mass Spectrometry (GC-MS) analysis, 41 volatile compounds were obtained in CC. AR could efficiently increase the contents of nitrogen oxides and methyl compounds. Meanwhile, the content of trans-.alpha.-bergamotene, nonanal, and copaene were significantly increased after the AR process (p < 0.05). According to the results of analysis of variance (ANOVA), odor activity value (OAV), and variable importance in projection (VIP), anethole was considered the key differential flavor-active compound. Overall, AR was superior to other reheating methods in CC, with better texture and various flavor characteristics. This study provides a reference for choosing reheating technology for pre-cooked chicken products. Full article

Protein hydrolysates positively affect intestinal function in both humans and animals, but their impact on gut health and the gut microbial profile in cats has not been thoroughly investigated. In this study, a total of 30 adult cats were randomly assigned to one of three dietary treatments for a 60-day feeding trial. The three dietary treatments were as follows: (1) basal diet (CON), (2) diet containing 15% powdered chicken protein hydrolysate (HP15%), and (3) diet containing 15% liquid chicken protein hydrolysate (HL15%). Compared to the CON group, the HP15% group had a decreased calprotectin levels and fecal gases emissions (p < 0.05). A higher abundance of Bacteroidota, Veillonellaceae, and Bacteroidaceae, while a lower abundance of Firmicutes was showed in the HL15% group than that in the CON group (p < 0.05). At the genus level, compared with the CON group, an increased abundance of Bacteroides spp. and Bifidobacterium spp. was showed, whereas a reduced abundance of Alloprevotella spp. was presented in the HP15% and HL15% groups (p < 0.05). The metabolomic analysis revealed 1405 distinct metabolites between the HP15% and CON groups (p < 0.05, VIP-pred-OPLS-DA > 1), and the level of cholic acid decreased while the level of isodeoxycholic acid increased in the HP15% group (p < 0.05). The metabolomic analysis revealed 1910 distinct metabolites between the HL15% and CON groups (p < 0.05, VIP-pred-OPLS-DA > 1), and the levels of 4-coumaryl alcohol and enterolactone increased in the HL15% group (p < 0.05). In summary, this study suggested that partially replacing chicken meat with chicken protein hydrolysate in the diet of cats helps regulate the gut microbial community and metabolite profile and improves intestinal health. Full article

Aphids (Hemiptera: Aphididae) are cosmopolitan generalist pests of many agricultural crops. Their ability to reproduce rapidly through parthenogenesis allows them to quickly reach population sizes that are difficult to control. Their damage potential is further exacerbated when they act as vectors for plant pathogens, causing diseases in plants. Aphids are typically managed through the widespread use of insecticides, increasing the likelihood of short-term insecticide resistance. However, for the past few decades, entomopathogenic bacteria have been used as an alternative management strategy. Entomopathogenic bacteria have demonstrated their effectiveness for biologically suppressing insect pests, including aphids. In addition to identifying bacterial species that are pathogenic to aphids, research has been conducted on toxins such as Cry, Cyt, Vip, recombinant proteins, and other secondary metabolites with insecticidal activity. Most studies on aphids have been conducted in vitro, exposing them to an artificial diet contaminated with entomopathogenic bacteria or bacterial metabolites for periods ranging from 24 to 96 h. The discovery of new bacterial species with insecticidal potential, as well as the possibility of biotechnological applications through the genetic improvement of crops, will provide more alternatives for managing these agricultural pests in the future. This will also help address challenges related to field application. Full article