Search Results (2,862)

Turbidity currents pose significant threats to offshore seabed infrastructures, including subsea hydrocarbon production facilities and submarine communication cables. These powerful underwater flows can damage pipelines, potentially causing hydrocarbon spills that endanger local communities, the environment, and negatively impact energy production infrastructures. Therefore, a comprehensive understanding of the spatio-temporal development and destructive force of turbidity currents is essential. While numerical computation of 3D flow, sediment transport, and substrate exchange is possible, field-scale simulations are computationally intensive. In this study, we develop a simplified morphodynamic approach to model the flow properties of channelized turbidity currents and the associated trends of sediment accretion and erosion. This model is applied to the Baco–Malaylay submarine system to investigate the dynamics of a significant turbidity current event that impacted a submarine pipeline offshore the Philippines. The modeling results align with available seabed assessments and observed erosion trends of the protective rock berm. Our simplified modeling approach shows good agreement with simulations from a fully 3D numerical model, demonstrating its effectiveness in providing valuable insights while reducing computational demands. Full article

Soil phosphorus (P) availability is an important determinant of productivity in Pinus massoniana (Masson pine) forests. The mechanistic bases governing the physiological and growth responses of Masson pine to varying soil P conditions remain insufficiently characterized. This study aims to decipher the adaptive strategies of Masson pine to different soil P levels, focusing on root morphological–architectural plasticity and the allocation dynamics of nutrient elements and photosynthetic assimilates. One-year-old potted Masson pine seedlings were exposed to four P addition treatments for one year: P0 (0 mg kg⁻¹), P1 (25 mg kg⁻¹), P2 (50 mg·kg⁻¹), and P3 (100 mg kg⁻¹). In July and December, measurements were conducted on seedling organ biomass, root morphological indices [root length (RL), root surface area (RSA), root diameter (RD), specific root length (SRL), and root length ratio (RLR) for each diameter grade], root architectural indices [number of root tips (RTs), fractal dimension (FD), root branching angle (RBA), and root topological index (TI)], as well as the content of nitrogen (N), phosphorus (P), carbon (C), and non-structural carbohydrates (NSCs) in roots, stems, and leaves. Compared with the P0 treatment, P2 and P3 significantly increased root biomass, root–shoot ratio, RL, RSA, RTs, RLR of finer roots (diameter ≤ 0.4 mm), nutrient accumulation ratio in roots, and starch (ST) content in roots, stems and leaves. Meanwhile, they decreased soluble sugar (SS) content, SS/ST ratio, C and N content, and N/P and C/P ratios in stems and leaves, as well as nutrient accumulation ratio in leaves. The P3 treatment significantly reduced RBA and increased FD and SRL. Our results indicated that Masson pine adapts to low P by developing shallower roots with a reduced branching intensity and promoting the conversion of ST to SS. P’s addition effectively alleviates growth limitations imposed by low P, stimulating root growth, branching, and gravitropism. Although a sole P addition promotes short-term growth and P uptake, it triggers a substantial consumption of N, C, and SS, leading to significant decreases in N/P and C/P ratios and exacerbating N’s limitation, which is detrimental to long-term growth. Under high-P conditions, Masson pine strategically prioritizes allocating limited N and SS to roots, facilitating the formation of thinner roots with low C costs. Full article

The cosmetics industry has been seeking to develop products with renewable natural ingredients to reduce the use of or even replace synthetic substances. Biosurfactants can help meet this demand. These natural compounds are renewable, biodegradable, and non-toxic or have low toxicity, offering minimal risk to humans and the environment, which has attracted the interest of an emerging consumer market and, consequently, the cosmetics industry. The aim of the present study was to produce a biosurfactant from the yeast Starmerella bombicola ATCC 22214 cultivated in a mineral medium containing 10% soybean oil and 5% glucose. The biosurfactant reduced the surface tension of water from 72.0 ± 0.1 mN/m to 33.0 ± 0.3 mN/m after eight days of fermentation. The yield was 53.35 ± 0.39 g/L and the critical micelle concentration was 1000 mg/L. The biosurfactant proved to be a good emulsifier of oils used in cosmetic formulations, with emulsification indices ranging from 45.90 ± 1.69% to 68.50 ± 1.10%. The hydrophilic–lipophilic balance index demonstrated the wetting capacity of the biosurfactant and its tendency to form oil-in-water (O/W) emulsions, with 50.0 ± 0.20% foaming capacity. The biosurfactant did not exhibit cytotoxicity in the MTT assay or irritant potential. Additionally, an antioxidant activity of 58.25 ± 0.32% was observed at a concentration of 40 mg/mL. The compound also exhibited antimicrobial activity against various pathogenic microorganisms. The characterisation of the biosurfactant using magnetic nuclear resonance and Fourier transform infrared spectroscopy revealed that the biomolecule is a glycolipid with an anionic nature. The results demonstrate that biosurfactant produced in this work has potential as an active biotechnological ingredient for innovative, eco-friendly cosmetic formulations. Full article

Background/objectives: This was a post hoc analysis of safety data across the bivalent respiratory syncytial virus prefusion F (RSVpreF) vaccine clinical trial development program. Methods: Data from eight clinical trials in 46,913 immunocompetent adults who received RSVpreF or placebo were analyzed. Local reactions and systemic events were assessed among non-pregnant ≥18-year-olds (n = 9517); adverse events (AEs) among pregnant and non-pregnant 18–59-year-olds (n = 9238); and vaccine-related AEs among non-pregnant ≥18-year-olds (n = 39,314). Post-marketing data in non-pregnant adults were considered. Results: Local reactions and systemic events were reported more frequently in RSVpreF versus placebo recipients; injection site pain was the most common local reaction (RSVpreF, 18.9%; placebo, 7.4%), and fatigue (23.5%; 18.4%) and headache (19.5%; 15.0%) were the most common systemic events. Percentages of AEs within 1 month after vaccination were similar across groups (RSVpreF, 12.8%; placebo, 13.1%); severe AEs were reported in ≤1.5% of participants. Differences in percentages of individuals reporting vaccine-related AEs between the RSVpreF and placebo groups were <0.2% for all related AEs. Serious AEs throughout the study were reported in ≤14.0% (RSVpreF, 12.6%; placebo, 14.0%). No atrial fibrillation, Guillain-Barré syndrome, or acute polyneuropathy cases were reported. The AE data from post-marketing data sources were consistent with the safety profile from the clinical trial program, with no new safety concerns. Conclusions: Integrated data demonstrated that RSVpreF was well tolerated with a favorable safety profile in non-pregnant and pregnant adults. Ongoing surveillance through real-world use and clinical trial experience continue to support the safety profile of RSVpreF. ClinicalTrials.gov: CT03529773/NCT04071158/NCT04785612/NCT05035212/NCT05096208/NCT05842967/NCT04032093/NCT04424316. Full article

Self-regulated learning (SRL) has been widely recognized as a critical skill for academic success in online and blended learning contexts. However, many students experience difficulty in effectively applying SRL strategies in the absence of structured instructional guidance. To address this challenge, this study developed and implemented a learning management system integrated with SRL support (SRL-LMS), specifically designed for the online component of a blended learning course. The SRL-LMS consisted of two sections: a conventional course content section and a SRL training section designed to support students in applying SRL strategies. A quasi-experimental design was adopted with 69 college students assigned to either an experimental group, with access to both course and SRL sections, or a control group, which accessed only the course section. Results indicated that students in the experimental group reported higher levels of self-regulation and showed more frequent and diverse application of SRL strategies compared to the control group. In terms of academic performance, the experimental group performed significantly better than the control group on the first exam, though no significant difference was observed on the second exam. These results highlight the effectiveness of structured SRL interventions within digital learning platforms for improving students’ self-regulatory behaviors. Future implementations should address cognitive load and incorporate strategic approaches to sustain student motivation. This study advances current SRL intervention designs and offers valuable insights for educators and instructional designers aiming to integrate targeted SRL supports in online and blended learning environments. Full article

Postharvest storage of kiwifruit requires the implementation of precise environmental conditions to maintain fruit quality and reduce decay. In this research, conducted over two years, we examined whether the storage conditions, characterized by low temperature (1 ± 1 °C) and ultra-high relative humidity (higher than 99%, close to saturation), generated by the Xedavap^® machine from Xeda International, were effective in maintaining the fruit quality and reducing postharvest rots compared to standard storage conditions, characterized by involved low temperature (1 ± 1 °C) and high relative humidity (98%). Kiwifruits preserved under the experimental conditions exhibited a significantly lower rot incidence after 60 days of storage, with the treated fruits showing 4.48% rot compared to 23.03% under the standard conditions in the first year, using inoculated fruits, and 6.30% versus 9.20% in the second year using naturally infected fruits, respectively. After shelf life (second year only), rot incidence remained significantly lower in the treated fruits (12.80%) compared to the control (42.30%). Additionally, quality analyses showed better parameters when using the Xedavap^® system over standard methods. The ripening process was effectively slowed down, as indicated by changes in the total soluble solids, firmness, and titratable acidity compared to the control. These results highlight the potential of ultra-high relative humidity conditions to reduce postharvest rot, extend the shelf life, and enhance the marketability of kiwifruit, presenting a promising and innovative solution for the horticultural industry. Full article

Background: Since the COVID-19 pandemic, managing acute infections in symptomatic individuals, regardless of vaccination status, has been widely debated and extensively studied. Even more concerning, however, is the impact of COVID-19 on pregnant women—especially its effects on fetuses and newborns. Several studies have documented complications in both expectant mothers and their infants following infection. Methods: In our previous works, we provided scientific evidence of the bacteriophage behavior of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2). This demonstrated that a well-defined combination of two antibiotics, amoxicillin and rifaximin, is associated with the same statistics for subjects affected by severe cases of SARS-CoV-2, regardless of vaccination status. We considered the few cases in the literature regarding the management of pregnancies infected with SARS-CoV-2, as well as previous data published in our works. In this brief case series, we present two pregnancies from the same unvaccinated mother—one prior to the COVID-19 pandemic and the other during the spread of the Omicron variant—as well as one pregnancy from a mother vaccinated against COVID-19. We describe the management of acute maternal infection using a previously published protocol that addresses the bacteriophage and toxicological mechanisms associated with SARS-CoV-2. Results: The three pregnancies are compared based on fetal growth and ultrasound findings. This report highlights that, even in unvaccinated mothers, timely and well-guided management of symptomatic COVID-19 can result in positive outcomes. In all cases, intrauterine growth remained within excellent percentiles, and the births resulted in optimal APGAR scores. Conclusions: This demonstrates that a careful and strategic approach, guided by ultrasound controls, can support healthy pregnancies during SARS-CoV-2 infection, regardless of vaccination status. Full article

(1) Background: Sertoli–Leydig cell tumors (SLCTs) are rare ovarian neoplasms that account for less than 0.5% of all ovarian tumors. They usually affect young women and often present with androgenic symptoms. We report a unique case of a 40-year-old woman diagnosed with both SLCT and clear cell papillary renal cell carcinoma (CCP-RCC), a rare tumor association with unclear pathogenesis. (2) Methods: Both tumors were treated surgically. The diagnostic workup included hormonal testing, imaging studies, and extensive genetic testing, including DICER1 mutation analysis and multiplex ligation-dependent probe amplification (MLPA), as well as the examination of a next-generation sequencing (NGS) panel covering ~280 cancer-related genes. (3) Results: Histopathologic examination confirmed a well-differentiated SLCT and CCP-RCC. No pathogenic variants in DICER1 were identified by WES or MLPA. No clinically relevant changes were found in the extended NGS panel either, so a known hereditary predisposition could be ruled out. The synchronous occurrence of both tumors without genomic alterations could indicate a sporadic event or as yet unidentified mechanisms. (4) Conclusions: This case highlights the importance of a multidisciplinary approach in the management of rare tumor compounds. The exclusion of DICER1 mutations and the absence of genetic findings adds new evidence to the limited literature and underscores the importance of long-term surveillance and further research into potential shared oncogenic pathways. Full article

This paper presents the design, simulation, and experimental validation of a dual-Circularly Polarized (CP) array antenna to be used as single element for a bistatic radar system, aimed at detecting and tracking objects in Low Earth Orbit (LEO). The antenna operates at 412 MHz in reception mode and consists of an array of 19 slotted-patch radiating elements with a cavity-based metallic superstrate, designed to support dual circular polarization. These elements are arranged in a hexagonal configuration, enabling the array structure to achieve a maximum realized gain of 17 dBi and a Side Lobe Level (SLL) below −17 dB while maintaining high polarization purity. Two identical analog feeding networks enable the precise control of phase and amplitude, allowing the independent reception of Right-Hand and Left-Hand Circularly Polarized (RHCP and LHCP) signals. Full-wave simulations and experimental measurements confirm the high performance and robustness of the system, demonstrating its suitability for integration into large-scale Space Situational Awareness (SSA) sensor networks. Full article

Phycocyanin is a natural, non-toxic, blue pigment-protein with many commercial applications. Its exploitation in various biotechnological sectors strongly depends on its purity grade (P). Phycocyanin is largely used in food industry where a low purity grade is required, while its widespread use in sectors requiring a higher purity is hampered by the cost of large-scale industrial production. Industry, in fact, needs simple, easily scalable and cost-effective procedures to ensure sustainable production of high-quality pigment. In this work we applied the innovative two-step ultrasound-based process UltraBlu to the pilot-scale production of phycocyanin. A total of 50 L of biomass suspension of commercial Spirulina were processed in batch mode. The pigment extract was obtained in one day, including the biomass harvesting. Food/cosmetic grade (P = 1.41–1.76) and a good yield (Y = 59.2–76.1%) were achieved. The initial results obtained suggest that UltraBlu can be an effective scalable process suitable to produce phycocyanin also on an industrial scale. Full article

Real-time positioning is a technological field with a multitude of applications, which expand across many scopes: from positioning within a large area to localization within smaller spaces; from locating people to locating equipment; from large-scale industrial or military applications to commercially available solutions. There are at least as many implementations of real-time positioning as there are applications and challenges. Within the domain of Radio Frequency (RF) systems, positioning has been approached from multiple angles. Some of the more common solutions involve using Time of Flight (ToF) and time difference of arrival (TDoA) technologies. Within TDoA-based systems, one common limitation stems from the computational power necessary to run the multi-lateration algorithms at a high enough speed to provide high-frequency refresh rates on the tag positions. The system presented in this study implements a complete hardware and software TDoA-based real-time positioning system, using wireless Ultra-Wideband (UWB) technology. This system demonstrates improvements in the state of the art by addressing the above limitations through the use of a hybrid Machine Learning solution combined with algorithmic fine tuning in order to reduce computational power while achieving the desired positioning accuracy. This study presents the design, implementation, verification and validation of the aforementioned system, as well as an overview of similar solutions. Full article

Achieving optimal agricultural yields and promoting sustainable farming relies on accurate crop recommendations. However, the applicability of many current systems is limited by their considerable computational requirements and dependence on comprehensive datasets, especially in resource-limited contexts. This paper presents HOLISTIQ RS, a novel crop recommendation system explicitly designed for operation on low-specification hardware and in data-scarce regions. HOLISTIQ RS combines collaborative filtering with a Markov model to predict appropriate crop choices, drawing upon user profiles, regional agricultural data, and past crop performance. Results indicate that HOLISTIQ RS provides a significant increase in recommendation accuracy, achieving a MAP@5 of 0.31 and nDCG@5 of 0.41, outperforming standard collaborative filtering methods (the KNN achieved MAP@5 of 0.28 and nDCG@5 of 0.38, and the ANN achieved MAP@5 of 0.25 and nDCG@5 of 0.35). Significantly, the system also demonstrates enhanced recommendation diversity, achieving an Item Variety (IV@5) of 23%, which is absent in deterministic baselines. Significantly, the system is engineered for reduced energy consumption and can be deployed on low-cost hardware. This provides a feasible and adaptable method for encouraging informed decision-making and promoting sustainable agricultural practices in areas where resources are constrained, with an emphasis on lower energy usage. Full article

This paper presents a novel interface that leverages a smartwatch for controlling industrial robotic arms. By harnessing the gyroscope and advanced gesture recognition capabilities of the smartwatch, our solution facilitates intuitive, real-time manipulation that caters to users ranging from novices to seasoned professionals. A dedicated application is implemented to aggregate sensor data via an open-source library, providing a streamlined alternative to conventional control systems. The experimental setup consists of a smartwatch equipped with a data collection application, a robotic arm, and a communication module programmed in Python. Our aim is to evaluate the practicality and effectiveness of smartwatch-based control in a real-world industrial context. The experimental results indicate that this approach significantly enhances accessibility while concurrently minimizing the complexity typically associated with automation systems. Full article

Background/Objectives: “Ozempic face” is an aesthetic side effect associated with the use of the antidiabetic agent Ozempic (semaglutide), characterized by a prematurely aged and fatigued facial appearance due to rapid weight loss. Currently, treatment options for this condition are limited. In this study, we present our clinical experience with the BodyTite device, provided by InMode Italy S.r.l. (Rome, Italy). Materials and Methods: We report a case series involving 24 patients (19 women and 5 men, aged 27–65 years), treated with subdermal bipolar radiofrequency (Endotissutal Bipolar Radiofrequency) between 2023 and 2024. All patients underwent a minimum follow-up of 12 months. At the end of the follow-up period, patients rated their satisfaction on a from 0 to 10 scale, and an independent expert assessed the stability of clinical outcomes. Results: The majority of patients reported high satisfaction levels (≥8), which correlated with the independent expert’s evaluation of treatment efficacy and result stability. The only observed adverse event was transient cutaneous erythema. Conclusions: “Ozempic face” is an increasingly common side effect associated with newer classes of antidiabetic medications. Although these drugs offer significant metabolic benefits, the accompanying facial volume loss and aging are often poorly tolerated by patients. Our findings suggest that subdermal bipolar radiofrequency represents a safe, low-risk, and cost-effective therapeutic option for the aesthetic management of Ozempic face. Full article

The CRISPR/Cas9 system is a powerful genome-editing tool that is applied in baculovirus engineering. In this study, we present the first report of the AcMNPV genome deletions for bioproduction purposes, using a dual single-guide RNA (sgRNA) CRISPR/Cas9 approach. We used this method to remove nonessential genes for the budded virus and boost recombinant protein yields when applied as BEVS. We show that the co-delivery of two distinct ribonucleoprotein (RNP) complexes, each assembled with a sgRNA and Cas9, into Sf9 insect cells efficiently generated deletions of fragments containing tandem genes in the genome. To evaluate the potential of this method, we assessed the expression of two model proteins, eGFP and HRPc, in insect cells and larvae. The gene deletions had diverse effects on protein expression: some significantly enhanced it while others reduced production. These results indicate that, although the targeted genes are nonessential, their removal can differentially affect recombinant protein yields depending on the host. Notably, HRPC expression increased up to 3.1-fold in Spodoptera frugiperda larvae. These findings validate an effective strategy for developing minimized baculovirus genomes and demonstrate that dual-guide CRISPR/Cas9 editing is a rapid and precise tool for baculovirus genome engineering. Full article