Search Results (3,373)

LED lamps have not been demonstrating the durability claimed by their manufacturers. One hypothesis is that switching transients may contribute to this. This study investigated switching-induced transients in LED lamps operated through dry contacts: manual switches and contactors. Using an oscilloscope, automated acquisition of waveform records was performed while several lamps were switched on in a 220 V_RMS/60 Hz electrical network. LED lamps of different models and manufacturers, one incandescent lamp, and a group of 48 LED lamps, subdivided into six sets of eight lamps, were all switched simultaneously. A total of 56 waveform-record files were obtained from the oscilloscope, comprising 2920 captured screens and 170 measurements. Transient voltage peaks of 380 and 391 V at the supply side, and 357 and 370 V at the lamp side, as well as voltage slew rates of up to 12 and 13 V/µs at the supply side and up to 16 and 19.5 V/µs at the lamp side, were measured, without considering statistical variations, which may indicate values exceeding the ordinary sinusoidal voltage peak (≅311 V) and its typical worst-case slew rate (≅0.12 V/µs). Future studies are suggested, such as tests in real installations, investigations of transient amplification or attenuation within electrical networks, assessment of the effects of wiring and impedance discontinuities, switch bounce, and semiconductor degradation, among others, to continue these studies. Full article

Chitinases catalyze the hydrolysis of β-1,4-glycosidic bonds in chitin, a structural biopolymer synthesized by numerous organisms. Although these enzymes have been widely investigated, studies focusing on insect-derived chitinases remain limited. In this study, three recombinant chitinases from the leaf-cutter ant Atta sexdens were cloned, expressed in Pichia pastoris, and biochemically characterized. The enzymes-AsChtII-C2B3 (one catalytic and three chitin-binding domains), AsChtII-C3C4 (two catalytic domains), and AsChtII-C5B1 (one catalytic and one binding domain), exhibited optimal activity at pH 4–5 and 50 °C using colloidal chitin as substrate. Chitinase activity on colloidal α-chitin was confirmed by ¹H NMR (proton nuclear magnetic resonance) spectroscopy, revealing GlcNAc concentrations of 0.41, 0.48, and 0.56 mmol L⁻¹ for AsChtII-C3C4, AsChtII-C2B3, and AsChtII-C5B1, respectively. Their antifungal activities were evaluated against the human pathogens Candida albicans and Aspergillus fumigatus, as well as the phytopathogen Lasiodiplodia theobromae. Distinct inhibition profiles were observed: AsChtII-C5B1 (150 µg/mL) showed the highest activity against C. albicans (87.6% inhibition), while AsChtII-C3C4 (25 µg/mL) was most effective against A. fumigatus (60% inhibition). Notably, only AsChtII-C2B3 inhibited L. theobromae growth, inducing severe hyphal deformations observed by scanning electron microscopy (SEM). These findings demonstrate that recombinant A. sexdens chitinases exhibit species-specific antifungal properties, underscoring their potential as biotechnological tools for medical and agricultural applications. Full article

Arc welding techniques for applying austenitic stainless steel cladding to low-carbon steels are common. Cladding enhances surface properties, increases corrosion resistance, improves product performance, extends service life, and reduces maintenance costs associated with surface corrosion. The hot-wire gas tungsten arc welding (HW-GTAW) method offers several benefits, making it appealing for cladding applications. This research investigates the use of HW-GTAW to clad low-carbon steels with super-austenitic stainless steel, examining macro and microstructures, mechanical strength, corrosion resistance, and wear performance. Two conditions were tested: one without a hot-wire, called CW-GTAW (cold-wire), and one with a hot-wire, called HW-GTAW. The HW-GTAW process reduced the dilution rate, thereby benefiting cladding. Microstructural analysis showed that both conditions exhibited elongated columnar dendrites in the heat-affected zone and a shallow region of equiaxed dendrites near the surface. The HW-CL condition displayed slight improvements in corrosion and wear resistance, but both samples outperformed the uncoated base material. These findings support the expanded application of super austenitic stainless steels and HW-GTAW in cladding processes. Full article

Background/Objectives: People with Parkinson’s disease (PwPD) exhibit impairments in postural responses to perturbations, increasing their risk of falls. While transcranial direct current stimulation (tDCS) has been shown to enhance postural responses in PwPD, its effects considering history of falls remain unclear. Thus, we aimed to analyse the effect of tDCS on postural responses after external perturbation in PwPD with and without a history of falls. Methods: Twenty-two PwPD were distributed into two groups—faller (n = 12) and non-faller (n = 10)—based on their history of falls over the 12 months preceding the experiment. A 20 min anodal tDCS was applied to the primary motor cortex (M1) under two conditions (2 mA and sham), performed on two different visits (at least 2 weeks apart) with a randomised order. Seven trials with temporally unpredictable external perturbation (i.e., backward translation of the support base) were performed after tDCS. Electromyographic (i.e., medial gastrocnemius (MG) onset latency, magnitude of muscle activation of MG and tibialis anterior (TA), and MG/TA coactivation index) and centre of pressure (CoP) parameters (i.e., range of CoP, peak of CoP velocity, and recovery time) were analysed to assess postural response. A two-way ANOVA (Group × Stimulation Condition) was performed. Results: Both groups had shorter recovery time (determined by CoP) and MG onset latency in the active vs. sham condition. Conclusions: The results of our pilot study suggest that a single 20 min tDCS session (2 mA) applied over M1 enhances postural responses similarly in PwPD with and without a history of falls in the past year. Full article

Kappaphycus alvarezii is an important source of carrageenan, a polysaccharide widely utilized for its gelling and stabilizing properties. However, understanding advancements in its application is crucial for broadening its biotechnological uses and promoting sustainable practices. This study aimed to conduct a systematic review of the applications of carrageenan from K. alvarezii, following PRISMA guidelines. A search was conducted in the CAPES Journals Portal and Scopus databases from 2010 to 2025, using the descriptors “Kappaphycus alvarezii” and “carrageenan.” Out of 491 analyzed articles, 38 met the inclusion criteria, categorized into health/medicine (n = 11), human food (n = 10), general industry (n = 8), animal nutrition (n = 6), and agriculture (n = 3). The findings reveal various applications, including scaffolds, antimicrobial agents, encapsulants, and wound dressings in health/medicine; edible films and food additives in human food; biomaterials and bioproducts, as well as applications in biorefinery in general industry; applications in aquaculture and livestock in animal nutrition; and as a defense inducer or biostimulant in agriculture. Despite a limited number of articles specifically addressing the direct applications of carrageenan from K. alvarezii, its uses are extensive across various industries. Full article

This study assesses the presence and distribution of per- and polyfluoroalkyl substances (PFAS) and polybrominated diphenyl ethers (PBDEs) in the surface waters of the Itupararanga Reservoir and the Sorocaba River, Brazil. Samples collected during the dry and rainy seasons were analyzed to determine their composition, spatial distribution, and seasonal variability. Results indicate the ubiquitous presence of PFAS, with significantly higher concentrations in the dry season, suggesting point sources of contamination, such as industrial and domestic discharges. Perfluorobutanoic acid (PFBA), Perfluorooctane sulfonate (PFOS), and Perfluorooctanoic acid (PFOA) were the predominant compounds, while 6:2 Fluorotelomer sulfonate (6-2FTS) stood out for its abundance in areas with industrial activity. For PBDEs, marked seasonal variability was observed, with higher concentrations during the rainy season, suggesting the mobilization of these compounds by surface runoff. BDE-209 was the most abundant congener, representing over 58% of the total concentration of PBDEs detected. Concentrations of PFAS and PBDEs in the study area are comparable to those reported globally, although there are differences associated with industrial practices and local environmental dynamics. The increased presence of short-chain PFAS and Deca-BDEs highlights the need for ongoing monitoring and the implementation of regulatory measures to mitigate contamination in water sources used for human consumption. Full article

Eggshell waste represents an underutilized biogenic resource, rich in calcium carbonate and organic components, whose increasing generation poses significant environmental and economic challenges. It is estimated that more than 8 million tons of eggshells are produced worldwide annually, most of which are disposed of in landfills, leading to landfill overload and increased waste management costs. In this context, the main objective of this study was to develop and compare three processing routes for converting calcium carbonate derived from eggshell waste into calcium acetate, using commercial vinegar (4–5% acetic acid) as a low-cost, sustainable acid source. The proposed routes were systematically evaluated based on processing efficiency, product characteristics, and operational simplicity. The materials obtained were characterized by X-ray fluorescence, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA/DTG), X-ray photoelectron spectroscopy (XPS), particle size analysis, and zeta potential measurements. The results indicated that the eggshells contained approximately 95.39% calcium, confirming their suitability as an efficient calcium precursor. Among the routes evaluated, the process using a spherical condenser under reflux at 90 °C showed superior performance, achieving shorter reaction times and maintaining comparable yields of membrane byproducts compared to the other methods. This approach differs from conventional routes by eliminating the need for high-purity reagents, reducing processing time, and using exclusively food-grade acetic acid, thus increasing sustainability and cost-effectiveness. Morphological, chemical, and thermal analyses confirmed the quality and stability of the synthesized calcium acetate. In general, these results demonstrate that eggshell waste can be efficiently valorized into calcium acetate through a scalable, straightforward process, thereby reducing landfill disposal and supporting circular economy strategies, with potential applications in the production of calcium-based biomaterials. Full article

Osteoarthritis (OA) is a degenerative joint disease that affects humans and animals worldwide. Its early diagnosis remains challenging due to subtle clinical signs and late radiographic changes. This study aimed to explore candidate biomarkers associated with spontaneous OA and to investigate their correlation with ultrasonographic scores to support early diagnosis. Clinical, radiographic, and ultrasonographic evaluations were performed on 52 equine metacarpophalangeal joints, with and without OA, allowing joint scoring and classification into osteoarthritis (OAG) and control groups. Synovial fluid samples were analyzed for cartilage degradation (C2C), untargeted ¹H NMR-based metabolomics, and lipid peroxidation (TBARS). Statistical analyses included Student’s t-test, Mann–Whitney U test, univariate and multivariate metabolomic analyses, and Spearman’s correlation (p < 0.05). Ultrasonography revealed higher scores in the synovial fold, membrane, and fluid, indicating synovitis as the predominant finding in the acute phase. C2C and TBARS concentrations were significantly higher in the OAG. Seven metabolites differed between groups, with citrate and TBARS showing the strongest correlations with ultrasonographic scores. These findings suggest increased metabolic activity and lipid peroxidation in early OA and highlight citrate and TBARS as potential auxiliary biomarkers for early diagnosis associated with synovitis. Full article

Background: Obesity-associated inflammation arises from adipose dysfunction and intestinal disturbances, including altered microbiota and short-chain fatty acid (SCFA) metabolism. Beans (Phaseolus vulgaris) are rich in non-digestible carbohydrates and polyphenols, but whether kidney bean varieties differing in seed coat colour exert distinct effects on inflammation in obesity remains unclear. Objective: To determine whether supplementation of an obesogenic high-fat (HF) diet with white or dark red kidney beans modulates gut microbiota, SCFAs, and intestinal, systemic, and neuroinflammatory outcomes. Methods: Male C57Bl/6N mice (n = 12/group) were fed a basal diet (BD; modified AIN-93G), an HF diet (60% kcal from fat), or an HF diet supplemented with 15% cooked white (HF + WK) or dark red kidney beans (HF + DK) for nine weeks. Outcomes included cecal microbiota composition, predicted KEGG pathways with taxon contributors mapped with BURRITO (a tool for linking predicted microbial functions to contributing taxa), and SCFA-related pathways; cecal and fecal SCFA concentrations; colon histomorphometry and expression of gut barrier junction and inflammatory genes; serum cytokines and adipose hormones; and hippocampal inflammatory and barrier genes. Results: Mice consuming bean-supplemented HF diets had higher microbial diversity, enrichment of SCFA-producing taxa (Prevotella, Lactobacillus, Muribaculaceae), and lower obesity-associated genera versus HF alone (Mucispirillum, rc4-4). Bean diets elevated cecal acetate and butyrate concentrations, which aligned with increases in predicted acetate kinase in both bean groups versus HF and BD, and butyrate kinase in HF + DK versus BD. Bean supplementation attenuated HF-induced reduction of goblet cells and systemic interleukin (IL)-10. The HF + DK group had lower colonic tumour necrosis factor (TNF)-α and partially attenuated hippocampal IL-6. SCFAs were inversely associated with systemic and neuroinflammatory markers in HF + DK mice. Conclusions: Kidney bean supplementation mitigated HF diet-induced intestinal, systemic, and neuroinflammatory disturbances in male mice, with microbiota and SCFA modulation. Further, dark red beans exerted stronger anti-inflammatory effects, highlighting the role of seed coat colour in bean-mediated obesity outcomes. Full article

Table grapes are among the main fruit crops cultivated in Brazil, supported by cultivar diversity, technological advances, and adaptation to diverse edaphoclimatic conditions. Rootstock selection is critical in viticulture, influencing phenology, yield, and fruit quality. This study evaluated yield- and fruit-related production parameters, cluster characteristics, and biochemical composition of ‘BRS Núbia’ table grape grafted onto different rootstocks. The experiment was conducted at the Experimental Farm of the Faculty of Agricultural Sciences (UNESP), São Manuel, São Paulo, Brazil, using a randomized block design in a split-plot scheme (three rootstocks × three seasons) with seven replicates. Rootstocks included ‘IAC 572 Jales’, ‘IAC 766 Campinas’, and ‘Paulsen 1103’, while subplots corresponded to the first three production seasons after grafting. Evaluated variables comprised bud fruitfulness, yield, productivity, physical attributes of clusters, berries, and rachises, and in 2022, berry biochemical traits, including total phenolics, flavonoids, anthocyanins, and antioxidant activity. Rootstocks did not significantly affect bud fruitfulness or yield-related parameters. In contrast, production season markedly influenced vine performance, with the third (2023) season showing higher cluster and berry mass and size. Regarding fruit composition, vines grafted onto ‘Paulsen 1103’ and ‘IAC 766 Campinas’ showed greater accumulation of total phenolics and anthocyanins than those grafted onto ‘IAC 572 Jales’, overall. Full article

The lack of interest in the reuse of phosphorus in agriculture is mainly due to the high abundance of pathogens, organic pollutants, microplastics, and possibly toxic metals. Therefore, different forms of treatment are necessary to take advantage of phosphorus recovery potential, one of which is the use of ash from incinerated/calcined biological sludge. A high rate of conversion of the non-apatite inorganic phosphorus fraction into apatite phosphorus was obtained in this study because of the use of commercially pure CaO additive in the dry sludge calcination tests, which is more bioavailable to plants. The obtained phosphorus pentoxide content ranged from 12 to 17%, surpassing several phosphorus-based raw materials and fertilizers. In addition, the ashes have been shown to contain toxic metals far below those recommended by Brazilian and international environmental legislation, so they can be applied directly to the soil for crop fertilization, or be used in P extraction and separation technologies for fertilizer production. Full article

Among Construction and demolition waste (CDW) has become a persistent challenge for urban sustainability, particularly in developing countries where institutional capacity and market coordination remain limited. While the reuse of CDW is widely recognized as technically feasible, its commercialization continues to face underlying obstacles. This research examines the factors limiting the consolidation of the CDW market in Maceió, Northeast Brazil, a city that recently experienced a large-scale geotechnical disaster and a sudden increase in CDW generation. The analysis is guided by the question: Which factors most strongly constrain the development of the CDW market in Maceió, and how do they interact? A mixed-methods design was adopted, combining survey data analyzed through the Relative Importance Index (RII), descriptive statistics, and ANOVA with semi-structured interviews involving professionals from construction, waste management, and public agencies. The results reveal five interconnected groups of barriers. The most influential are the absence of effective public policies (RII = 0.89), lack of fiscal incentives for recycling (RII = 0.88), fragmented legislation (RII = 0.87), and the systematic devaluation of recycled materials (RII = 0.85). Environmental constraints linked to land subsidence (RII = 0.90) further intensify market instability. Together, these findings show that CDW commercialization is shaped by interacting regulatory, economic, and cultural factors, underscoring the need for coordinated policy, fiscal, and governance responses in vulnerable urban contexts. Full article

Background/Objectives: Variation in repeatome composition is a major determinant of genome architecture and an important substrate for evolutionary change in plants. Despite the availability of genomic sequence data, repeatome-wide assessments have not been performed for Epidendrum, the largest Neotropical genus of Orchidaceae. Here, we assessed repeatome profiles across 34 Epidendrum species using publicly available genomic datasets. Methods:Epidendrum repeatomes were characterized with the RepeatExplorer2 pipeline, and patterns of repeat composition were evaluated for phylogenetic structure using a species phylogeny. Results: Repeat composition showed no clear phylogenetic structure, with closely related species often displaying divergent satDNA and TE profiles. satDNA content varied widely among species (15.5–69% of the repeatome fraction). A total of 208 satDNA families were detected, which were used to build a custom database for comparative analyses. We detected 73 satDNA clusters shared among species, whereas only three were species-specific. Regarding TEs, Class I elements were the most abundant repeats, dominated by Ty3-Gypsy LTR retrotransposons. Only two Class II TIR superfamilies were detected (EnSpm/CACTA and hAT). Conclusions: This study provides the first comprehensive characterization of the Epidendrum repeatome and establishes a resource for future work on cytogenomic diversity within this megagenus. The heterogeneous distribution of repeats among closely related species is consistent with lineage-specific amplification and loss, highlighting rapid repeatome turnover in Epidendrum. Potential drivers, as hybridization and ecological differentiation, should be tested explicitly in future analyses integrating broader genome size sampling and trait data. Full article

Objectives: We evaluated the effect of diacerein, an anti-inflammatory drug, on the activity and survival of alveolar bone osteoblasts in rats with periodontitis. Methods: The rats with periodontitis received diacerein (PDG) or saline solution (PSG) for 7, 15 and 30 days. In gingiva samples, Nfkb1 and Bmp2 gene expressions were evaluated, and maxillae were processed for light and transmission electron microscopy. Results: In PDG, the tumor necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β) immunoexpression decreased in parallel with the increase in alkaline phosphatase (ALP) and bone area over time. At 15 and 30 days, Nfkb1 expression decreased in PDG compared to PSG, whereas at 30 days, the Bmp2 expression was greater in PDG than in PSG. Immunofluorescence for IL-10, an anti-inflammatory cytokine, was greater in PDG than in PSG at 15 and 30 days. In PSG, the significant increase in the number of TUNEL-positive osteoblasts was accompanied by the presence of osteoblasts with condensed chromatin nuclei or caspase-3-immunolabelled osteoblasts. In contrast, the number of TUNEL-positive osteoblasts was significantly lower in PDG than in PSG specimens at all time points. Conclusions: Therefore, the diacerein-induced TNF-α and IL-1β inhibitory effect caused Nfkb1 downregulation and, hence, prevented apoptosis in osteoblasts. The increased ALP activity and IL-10 in PDG indicate that diacerein mitigates periodontitis impact on alveolar bone in rat molars. Full article

This study aimed at developing in vitro propagation methods for Crocus sativus L., focusing on the effectiveness of temporary immersion systems (TIS) or bioreactors as an alternative, cost-efficient technique for the large-scale production of saffron corms. The effects of the culture system and cross-cutting on saffron propagation were evaluated. Saffron shoots were cultured in TIS and compared with shoots produced using a conventional semi-solid tissue culture system (SS). The recipient material for automated temporary immersion used in this study was the SETIS™ bioreactor. The growth parameters measured for in vitro culture were the number of neo-formed shoots, shoot height, and the number and size of corms. Based on the present detailed study, the highest shoot multiplication rate (9.1 shoots/explant with 7.2 cm of shoot height) was achieved in the TIS system after shoot cross-cutting, while the lowest multiplication rates were obtained in the semi-solid system (1 shoot/explant with 14.8 cm long shoots). Furthermore, the highest corm formation was obtained in the TIS system, with an average of four corms per explant, with a larger corm weight (10.90 g) and diameter (21.78 mm). These findings highlighted for the first time the efficiency of the bioreactor system combined with cross-cutting of the shoot for efficient and scalable saffron corm propagation, thus making a valuable contribution to sustainable cultivation and conservation strategies while meeting the growing demand for this spice. Full article