Search Results (32)

The need for reliable biological indicators to support soil biomonitoring is increasing, particularly in agricultural systems where management practices and environmental pressures interact to influence soil ecological integrity. Among soil fauna-based indicators that measure soil health, the Soil Biological Quality indices based on microarthropods (QBS-ar) and earthworms (QBS-e) provide functional measures of soil condition, however their combined application remains largely unexplored. In this study, for the first time both indices were applied simultaneously to assess soil biological quality in the same agroecosystem. The context was that of contrasting agricultural systems (strip cropping vs. pure stands) in a real-farm experimental set-up. Additional biological variables and soil physico-chemical parameters were also considered. Statistical analyses included Spearman correlations, linear mixed-effect models (LMMs), and redundancy analysis (RDA). QBS-ar and QBS-e showed contrasting responses to management systems, with higher QBS-ar values under strip cropping and higher QBS-e values in pure stands. No significant relationship was observed between the two indices. Multivariate analyses indicated that both indices were significantly influenced by management and environmental variables, although with different patterns of association. QBS-ar appeared more responsive to variation in topsoil conditions, whereas QBS-e was associated with broader soil properties and seasonal community dynamics. These results indicate that QBS-ar and QBS-e capture complementary aspects of soil biological quality and should not be used interchangeably. Their combined use improves the interpretation of soil biological responses to agricultural management and associated environmental pressures. Overall, this study highlights the potential of soil fauna-based indices as practical tools for biomonitoring in agroecosystems and supports further exploration on the comparative responses of biological indexes. Full article

(1) Background: Soil fertility in organic systems depends on interactions between physicochemical properties and biological processes that regulate nutrient availability along the soil profile. However, information on their vertical distribution remains limited, particularly for root crops such as sugar beet. This study evaluated depth-related patterns in soils from three organic farms growing sugar beet. (2) Methods: Soil profiles (0–120 cm) were sampled and analyzed for physicochemical properties, mineral nitrogen (N) forms, and biological indicators, including the QBS-ar index, microbial abundance, and functional genes involved in N and carbon cycling. (3) Results: Nitrate-N and total mineral N were mainly concentrated in the 0–40 cm layer and declined markedly with depth. Microbial abundance and most N-cycling functional genes were similarly enriched in the topsoil, showing clear vertical stratification. Statistical analyses suggested that functional gene composition was associated with mineral N gradients after accounting for soil depth. (4) Conclusions: These findings provide an exploratory indication of relationships between mineral N forms and microbial indicators in an organically managed sugar beet system. Given the limited number of sampling units, results should be interpreted cautiously. However, these results highlight the value of soil profile approaches for understanding N redistribution and improving nutrient management strategies. Full article

Soil is essential for human survival, with approximately 95% of global food production originating from land. However, over the past century, overexploitation has led to soil degradation and biodiversity loss, with significant impacts on agroecosystems. Portuguese agriculture faces diverse challenges, particularly in the horticultural sector, which occupies substantial territory and supports key economic chains. Consequently, indicators for assessing soil quality are crucial, with mesofauna serving as sensitive bioindicators due to their ecosystemic roles. Among sustainable practices, cover crops are believed to mitigate soil issues by enhancing the biotic functionalities. This study aimed to evaluate the impact of cover crops on soil biological quality in horticultural systems in Portugal. From 2022 to 2025, six horticultural fields in the Alentejo, Ribatejo, and Oeste regions were assessed, introducing cover-crops before main crops and comparing them to controls. Soil samples were collected during cover and main crop presence; mesofauna was extracted via Berlese-Tullgren funnels and classified under the QBS-ar methodology. Results showed enhanced soil biological quality (p < 0.001) in cover crop plots compared to controls, with no significant differences across regions (p = 0.66) or crop types (p = 0.37), indicating the implementation of cover crops as the primary driver for enhanced soil health. Full article

Soil health and agricultural sustainability are primarily threatened by organic matter depletion and a decline in biodiversity. To mitigate these processes, managing set-aside systems remains a simple practice to preserve biodiversity and functions essential to soil fertility. The objective of this study was to evaluate the effectiveness of medium-term set-aside management in preventing soil degradation using changes in microbial and microarthropod communities, both of which are involved in organic matter degradation. Three different set-aside managements, with mowing (May or July) and without mowing, were compared to conventional rotation in three sites located in North, Central, and South Italy. The microbial community was analyzed through both biochemical methods, such as the assessment of soil respiration and microbial biomass, and molecular techniques, such as Denaturing Gradient Gel Electrophoresis. Soil fertility was assessed by the Biological Fertility Index, while soil biodiversity was assessed with microbial and microarthropod indicators. All set-aside management showed a statistically significant separation from conventional crop rotation for both microbial and microarthropod communities. Indicators highlighted a lower efficiency of set-aside management in organic matter use compared to conventional crop rotation, while microbial and microarthropod biodiversity increased in all set-aside managements. The QBS-ar index showed good soil quality standards in each set-aside management, benefiting the euedaphic microarthropods, whereas soil microbial respiration highlighted a higher microbial activity in the same experimental context. Full article

Soil biodiversity is crucial for maintaining biological soil resilience, understood as a temporal property and as the ability of soils to uphold or recover their ecological functions under stress thanks to the diversity and complementarity of their biological communities. To evaluate this property, we developed the Biological Soil Resilience Index (BSR), conceived as an evolution of the QBS-ar approach by integrating additional key bioindicators—entomopathogenic nematodes, entomopathogenic fungi, and earthworms—together with microarthropod eco-morphological adaptation scores. This multi-taxon framework provides a more comprehensive assessment of soil biological conditions than single-group indices and is specifically designed to be applied repeatedly over time to detect resilience trajectories. The Biodiversity Soil Resilience (BSR) Index was applied across nine sites subject to low, medium, and high anthropogenic disturbance, spanning urban, industrial, and airport environments. Results revealed not a resilience gradient but a clear disturbance gradient: low-impact sites achieved the highest BSR values (52–59), reflecting diverse and functionally complementary assemblages; medium-impact sites maintained moderate BSR value (27–42), but displayed imbalances among faunal groups; and high-impact sites showed the lowest values, including a critically low score at C_HI (17.86), where entomopathogens were absent and earthworm populations reduced. Entomopathogenic organisms proved particularly sensitive, disappearing entirely under severe disturbance. The BSR was sensitive to environmental gradients and effective in distinguishing ecologically meaningful differences among soil communities. Because it can be repeatedly applied over time, BSR provides the basis for monitoring long-term resilience dynamics, detecting early warning signals, and support timely mitigation or restoration measures. Overall, the study highlights the pivotal role of biodiversity in sustaining soil resilience and supports the BSR Index as a simple yet integrative tool for soil health assessment and for future resilience monitoring in disturbed landscapes. Full article

The performance of lettuce, pepper, and chili pepper, and the biological soil quality, in a ground-mounted PV system under cultivation conditions typical of the Mediterranean environment of the Puglia region were evaluated. Microclimatic parameters, plant growth and yield response, soil quality assessed using the QBS-ar index, and land equivalent ratio (LER) were determined in three different cultivation areas: a cultivation area outside the photovoltaic plant but immediately adjacent to it (‘Control’); the inter-row area closest to the row of panels exposed to sunlight (‘Area close PV structure’); the inter-row area distant from the row of panels (‘Area distant PV structure’). Cumulated solar radiation, in particular during the summer growing cycles, was only slightly affected in the Area distant PV structure (1616 and 2130 MJ m⁻² for pepper and chili pepper, respectively, in the control area, in comparison to 1630 and 2044 MJ m⁻², in the Area distant PV structure), while it was strongly reduced in the Area close PV structure (883 and 1091 MJ m⁻² for pepper and chili pepper, respectively). In general, a reduction in air temperature and wind speed, as well as an increase in relative air humidity, was observed under PV conditions. On average, the evapotranspirative demand was reduced in the PV growing conditions compared to open field, with a more relevant effect in the sub-zone close to the photovoltaic structures, where cumulative ET0 was 28% and 34% lower than the Control in the pepper and chili pepper growing cycle, respectively. Lettuce growth was impaired by PV cultivation conditions, with an average reduction of 15% in plant height and 37% in marketable yield per plant, with no significant differences between the two sub-zones in the PV system. For pepper, the best growing conditions were observed in open field control compared to PV, but with differences related to the PV sub-zone. The plants grown in the Area distant PV structure were more negatively affected by the modified growing conditions, showing the lowest shoot and fruit fresh weight, the latter reduced by 51% compared to the Control; intermediate values were observed for these parameters in the Area close PV structure, with a less severe tendency to yield reduction. For chili pepper, both shoot and fruit fresh weight were lower in PV conditions, regardless of the sub-zone, with a reduction of 82% in yield per plant compared to the Control. However, despite the yield reductions, the LER was improved (1.60 and 1.40 in case of a lettuce + pepper or lettuce + chili pepper annual cropping program, respectively), highlighting a more efficient use of land, without negative or even ameliorative impacts on biological soil quality and biodiversity in terms of QBS-ar and microarthropods taxa abundance. Knowledge of the response of different crops under cultivation conditions typical of specific environments is necessary to define optimal cropping programs aimed at maximizing resource-use efficiency and land use. Full article

The wine industry faces increasing challenges related to authenticity, safety, and sustainability due to recurrent fraud, shifting consumer preferences, and environmental concerns. In this study, as part of the B.I.O.C.E.R.T.O project, we integrated blockchain technology with ultrasonic spectroscopy and soil quality data by using the arthropod-based Soil Biological Quality Index (QBS-ar) to enhance traceability, ensure wine quality, and certify sustainable vineyard practices. Four representative wines from the Marche region (Sangiovese, Maceratino, and two Verdicchio PDO varieties) were analyzed across two vintages (2021 and 2022). Ultrasound spectroscopy demonstrated high sensitivity in distinguishing wines based on ethanol and sugar content, comparably to conventional viscosity-based methods. The QBS-ar index was applied to investigate the soil biodiversity status according to the agricultural management practices applied in each vineyard, reinforcing consumer confidence in environmentally responsible viticulture. By recording these data on a public blockchain, we developed a secure, transparent, and immutable certification system to verify the geographical origin of wines along with their unique characteristics. This is the first study to integrate advanced analytical techniques with blockchain technology for wine traceability, simultaneously addressing counterfeiting, consumer demand for transparency, and biodiversity preservation. Our findings support the applicability of this model to other agri-food sectors, with potential for expansion through additional analytical techniques, such as isotopic analysis and further agroecosystem sustainability indicators. Full article

Background: Various routes of drug administration are available for psoriasis treatment. However, there is an urgent need for novel and improved therapeutic options. Hence, our study aimed to develop a nanostructured lipid carrier (NLC) gel of hesperidin (HPD) using a systemic QbD approach for an effective treatment of psoriasis. Methods: Initially, HPD-NLC was optimized with independent variables (drug content, amount of liquid lipid, total lipid, and surfactant concentration) using Box–Behnken Design to assess dependent variables (particle size, size distribution, and entrapment efficiency). HPD-NLC was developed using the high-shear homogenization technique. The characteristics of nanoformulation such as particle size, morphology [transmission electron microscopy (TEM) and differential scanning calorimetry (DSC)], crystallinity [powder X-ray diffraction (XRD)], and chemical interactions [Fourier transform infrared spectroscopy (FTIR)], the drug entrapment efficiency (%EE), and the drug release were investigated. Franz-diffusion cell was utilized to perform in vitro diffusion study, and an imiquimod-induced psoriasis model was used for in vivo study. Results: The optimized HPD-NLC exhibited a spherical shape with particle size of 125.7 nm, polydispersity index (PDI) of 0.36, and entrapment efficiency of 52.26% w/w. Further, different techniques validated the reduced crystallinity of the hesperidin. The in vitro diffusion study highlighted the sustained and anomalous diffusion of the drug from NLC gel. In the in vivo study, the HPD-NLC-Gel-treated group displayed normal skin with minimal keratosis, while the drug-loaded gel group exhibited signs of hyperkeratosis and parakeratosis signs. Conclusions: HPD-NLC gel showed promising advancement in nanotechnology-based psoriasis treatment and the results of this study open the door for the application of topical HPD-NLC-Gel clinically. Full article

The impact of agricultural practices and changes in land use on soil microarthropod communities was investigated by analyzing the data from 53 sample sites with five different land-use categories (natural habitats, orchards, arable crops, olive groves and vineyards). These datasets are a mix of published and unpublished studies and were analyzed using statistical techniques that enabled a determination and comparison of the values of the biological soil quality index for each of the five land-use systems based on the richness and community composition of the biological forms of the microarthropods identified (QBS-ar), and the chemical and physical properties of the soil [e.g., pH, soil organic carbon (SOC), dominant particle sizes present (e.g., clay) and texture] for each locality. Our results confirm that different agricultural practices diminish, in different ways, the dominance and variety of species present in soil microarthropod communities. Furthermore, these agroecosystem communities represent a sub-sample of the soil microarthropod communities found in natural habitats; presumably due to the stress factors experienced from impact(s) of the differing chemical and physical properties of the soils, and the resultant selection pressures placed upon the biological forms present in the soil. Full article

Background: Removal of large uraemic toxins is still a challenge. Haemodiafiltration (HDF) has produced some results, although large convective volume, optimal vascular access to increase the blood flow rate and strict water quality management are required. Medium cut-off, high-retention-onset membranes have been recently developed, introducing the concept therapy called expanded haemodialysis (HDx). Furthermore, vitamin E-coated membrane has potential beneficial effects on inflammation and oxidative stress. Methods: A prospective longitudinal multicentre study was conducted for 3 months among 24 chronic haemodialysis patients. Patients were randomly assigned into either HDF with high-flux membrane or HDx with Theranova or ViE-X membrane. The primary goal was to assess albumin loss among the three types of dialyzers. Secondary goals included assessment of depurative efficacy for uraemic toxins and clinical outcomes. Results: Mean albumin loss was significantly higher in patients undergoing HDx with Theranova membrane, without any difference in serum albumin concentration among the three groups. Instantaneous clearance of small and middle molecules was significantly higher in patients undergoing HDF, but we did not find differences in removal ratio and Kt/V. Reduction in the erythropoietin resistance index was observed in patients treated with ViE-X membrane due to their lower dialysis vintage. Conclusions: The higher albumin loss during HDx has no effects on pre-dialysis serum albumin. HDx with Theranova in the presence of lower session length, lower Qb, lower convective dose, and lower instantaneous clearance reached the same dialysis efficacy compared to HDF. Full article

The QBS-ar, based on the study of microarthropod community structure, is well known as a quick and low-cost indicator to monitor soil biological quality at the farm scale. Temperature fluctuations and other climate factors in European countries may indirectly influence soil microarthropod communities by altering resource availability and microhabitat conditions. In the context of the climate crisis, along with drought and erosion threats, especially in southern Europe, it is essential to define the limits and advantages of the QBS-ar index. We applied the QBS-ar index along a warm temperature gradient at three long-term experimental sites. Our results underlined that the QBS-ar is very sensitive for detecting soil quality and treatment effects. The results suggest that the choice of sampling season is a particularly vulnerable phase, especially for southern Mediterranean sites. Air temperature and cumulative precipitation, even in the months prior to sampling, are critical factors to consider when applying the QBS-ar index in European countries. Drought periods can negatively influence the results for soil microarthropod relative abundance; however, the presence of biological forms seems to provide useful information about the effects of treatments on soil quality. This paper lays the groundwork for scaled-up QBS-ar applications considering soils and several environmental characteristics of agroecosystems in Europe. The work can contribute to the development of applications of the index, facilitating and improving the monitoring of soil biology at the field scale. Furthermore, this study can open future perspectives for the application of QBS-ar on a larger scale thanks to the implementation and updating of an open-source database. Full article

Policies aimed at reducing plant protection products (PPPs) are part of the UN’s 2030 Agenda for Sustainable Development. Sustainable management of PPPs is crucial for soil health, biodiversity, and ecosystem services, including food provision. While PPPs can control pests and enhance agricultural yields, they also pose environmental and health risks by contaminating water, soil, and non-target organisms through airborne drift. Investigating innovative and more sustainable distribution methods can support sustainability goals. This study aimed to evaluate the potential impact of the pesticide Spintor^® Fly on non-target soil organisms in olive orchards comparing two spraying methods: a traditional Casotti^® pump mounted on a tractor and an innovative Unmanned Aerial Vehicle (UAV) developed for the project. The study was conducted in 2021 in an organic olive orchard, which was divided into two plots: a Casotti-treated plot (CAS) and a drone-treated plot (DRO). A strip of uncultivated land at the edge of the orchard was used as a (non-treated) control plot (CAP). The impact on native soil microarthropod communities was assessed using the arthropod-based Soil Biological Quality Index (QBS-ar) and Bait Lamina Test (BLT). Soil samples were collected for earthworm avoidance tests and soil chemical–physical analysis. The results obtained with QBS-ar and BLT indicated no significant differences between DRO and CAS, in both sampling periods (pre- and post-treatment). However, DRO generally exhibited slightly better performance than CAS. The avoidance behaviour was confirmed for both CAS and DRO, although it was lower for the latter. Overall, drone aerial spray performed slightly better, suggesting a potentially lower impact on soil communities. Our results provide initial clues for the sustainable use of drones in agriculture with no increased risks for soil health compared to traditional methods. Further long-term studies should be conducted to validate these findings and possibly confirm the long-term benefits of drone applications compared to traditional methods. Full article

Background: The ever-growing emergence of antibiotic resistance associated with tuberculosis (TB) has become a global challenge. In 2012, the USFDA gave expedited approval to bedaquiline (BDQ) as a new treatment for drug-resistant TB in adults when no other viable options are available. BDQ is a diarylquinoline derivative and exhibits targeted action on mycobacterium tuberculosis, but due to poor solubility, the desired therapeutic action is not achieved. Objective: To develop a QbD-based self-nanoemulsifying drug delivery system of bedaquiline using various oils, surfactants, and co-surfactants. Methods: The quality target product profile (QTPP) and critical quality attributes (CQAs) were identified with a patient-centric approach, which facilitated the selection of critical material attributes (CMAs) during pre-formulation studies and initial risk assessment. Caprylic acid as a lipid, propylene glycol as a surfactant, and Transcutol-P as a co-surfactant were selected as CMAs for the formulation of bedaquiline fumarate SNEDDS. Pseudo-ternary phase diagrams were constructed to determine the optimal ratio of oil and S_mix. To optimize the formulation, a Box–Benkhen design (BBD) was used. The optimized formulation (BDQ-F-SNEDSS) was further evaluated for parameters such as droplet size, polydispersity index (PDI), percentage transmittance, dilution studies, stability studies, and cell toxicity through the A549 cell. Results: Optimized BDQ-F-SNEDDS showed well-formed droplets of 98.88 ± 2.1 nm with a zeta potential of 21.16 mV. In vitro studies showed enhanced drug release with a high degree of stability at 25 ± 2 °C, 60 ± 5% and 40 ± 2 °C, 75 ± 5%. Furthermore, BDQ-F-SNEDDS showed promising cell viability in A549 cells, indicating BDQ-F-SNEDDS as a safer formulation for oral delivery. Conclusion: Finally, it was concluded that the utilization of a QbD approach in the development of BDQ-F-loaded SNEDDS offers a promising strategy to improve the biopharmaceutical properties of the drug, resulting in potential cost and time savings. Full article

Under the increasing global energy demand, the new European Union Biodiversity Strategy for 2030 encourages combinations of energy production systems compatible with biodiversity conservation; however, in photovoltaic parks, panels shadowing the effects on soil health and biodiversity are still unknown. This study (location: Northern Italy) aimed to evaluate the effect of ground-mounted photovoltaic (GMPV) systems on soil arthropod biodiversity, considering two parks with different vegetation management: site 1—grassland mowed with tractor; site 2—grassland managed with sheep and donkeys. Three conditions were identified in each park: under photovoltaic panel (row), between the panel rows (inter-row), and around the photovoltaic plant (control). The soil pH and organic matter (SOM), soil arthropod community, biodiversity, and soil quality index (e.g., QBS-ar index) were characterised. Differences between the two GMPVs were mainly driven by the SOM content (higher values where grazing animals were present). No differences were observed in site 1, even if a high heterogeneity of results was observed for the soil biodiversity parameters under the panels. In site 2, SOM and pH, as well as arthropods biodiversity and QBS-ar, showed low values in the row. Soil fauna assemblages were also affected by ground-mounted panels, where Acarina, Collembola, Hymenoptera, and Hemiptera showed the lowest density in the row. This study suggests that ground-mounted solar panels had significant effects on below-ground soil fauna, and was more marked depending on the system management. Furthermore, the results obtained for the inter-row were similar to the control, suggesting that the area between the panel rows could be considered a good hotspot for soil biodiversity. Full article

Perfluorocarbon nanoemulsions (PFC-NEs) are widely used as theranostic nanoformulations with fluorescent dyes commonly incorporated for tracking PFC-NEs in tissues and in cells. Here, we demonstrate that PFC-NE fluorescence can be fully stabilized by controlling their composition and colloidal properties. A quality-by-design (QbD) approach was implemented to evaluate the impact of nanoemulsion composition on colloidal and fluorescence stability. A full factorial, 12-run design of experiments was used to study the impact of hydrocarbon concentration and perfluorocarbon type on nanoemulsion colloidal and fluorescence stability. PFC-NEs were produced with four unique PFCs: perfluorooctyl bromide (PFOB), perfluorodecalin (PFD), perfluoro(polyethylene glycol dimethyl ether) oxide (PFPE), and perfluoro-15-crown-5-ether (PCE). Multiple linear regression modeling (MLR) was used to predict nanoemulsion percent diameter change, polydispersity index (PDI), and percent fluorescence signal loss as a function of PFC type and hydrocarbon content. The optimized PFC-NE was loaded with curcumin, a known natural product with wide therapeutic potential. Through MLR-supported optimization, we identified a fluorescent PFC-NE with stable fluorescence that is unaffected by curcumin, which is known to interfere with fluorescent dyes. The presented work demonstrates the utility of MLR in the development and optimization of fluorescent and theranostic PFC nanoemulsions. Full article