Search Results (64)

The color of flowers in Osmanthus fragrans is regulated by carotenoid metabolism. The orange-red variety, Dangui, is believed to have evolved from the yellow variety, Jingui, through a natural bud mutation. This study uses the Jingui cultivar ‘Jinqiu Gui’ (JQG) and its bud mutation cultivar ‘Huolian Jindan’ (HLJD) as materials, combining genome resequencing, ultrastructural observation, targeted metabolomics, and transcriptomic analysis to elucidate the molecular and cellular mechanisms underlying flower color variation. Phylogenetic analysis confirms that HLJD is a natural bud mutation of JQG. Ultrastructural observations reveal that during petal development, chromoplasts are transformed from proplastids. In HLJD petals, starch granules degrade more slowly and exhibit abnormal morphology, resulting in chromoplasts displaying crystalline, tubular, and fibrous composite structures, in contrast to the typical spherical plastoglobuli found in JQG. Targeted metabolomics identified 34 carotenoids, showing significant increases in the levels of ε-carotene, γ-carotene, α-carotene, and β-carotene in HLJD petals compared to JQG, with these levels continuing to accumulate throughout the flowering process, while the levels of the cleavage products α-ionone and β-ionone decrease. Transcriptomic analysis indicates that carotenoid metabolic pathway genes do not correlate directly with the phenotype; however, 49 candidate genes significantly associated with pigment accumulation were identified. Among these, the expression of genes such as glycoside hydrolases (LYG036752, etc.), sucrose synthase (LYG010191), and glucose-1-phosphate adenylyltransferase (LYG003610) are downregulated in HLJD. This study proposes for the first time the pathway of “starch degradation delay → chromoplast structural abnormalities → carotenoid cleavage inhibition” for deepening flower color, providing a new theoretical model for the metabolic regulation of carotenoids in non-photosynthetic tissues of plants. This research not only identifies key target genes (such as glycoside hydrolases) for the color breeding of O. fragrans but also establishes a theoretical foundation for the color enhancement of other ornamental plants. Full article

Ethylene-insensitive 3/ethylene-insensitive 3-like (EIN3/EIL) transcription factors are central regulators of ethylene signaling and stress adaptation in plants. However, their roles in Osmanthus fragrans, a globally cherished ornamental and aromatic plant with significant economic value, remain poorly characterized. Here, we identified nine OfEIL genes across eight chromosomes in the O. fragrans “Liuye Jingui” genome. Conserved motif analysis revealed core domains (Motif1/2/4/7), and promoter cis-elements highlighting hormone-related, stress-related, and growth-related regulatory potential. During late flowering stages, six OfEILs (3/4/5/6/7/9) were significantly upregulated. Under 5-azacytidine (AZA, a DNA demethylation agent), OfEIL2 and OfEIL7 were downregulated, whereas the ETH treatment activated OfEIL3/7/8/9. Strikingly, OfEIL7 exhibited dual regulatory roles, correlating strongly with natural flowering progression, AZA-induced demethylation, and ETH responses. Functional divergence was observed in petal senescence, with OfEIL2–5 and OfEIL7–9 showing stage-specific and tissue-specific expression patterns. These results position OfEIL7 as a key hub integrating epigenetic and hormonal signals to modulate floral longevity and stress adaptation. Our study provides the first genome-wide characterization of the EIL family in O. fragrans, offering critical insights for molecular breeding aimed at enhancing ornamental traits and environmental resilience in this economically significant species. Full article

Osmanthus fragrans is a highly valued ornamental tree species in China, but its short flowering period limits its ornamental appeal. Investigating the mechanisms of flower senescence in O. fragrans is therefore of significant importance. Ethylene, a key endogenous hormone, plays a central role in flower senescence, and the AP2/ERF gene family, which includes ethylene response factors, is known to regulate this process in various plants. Transcriptome sequencing and expression analysis identified OfERF17 as a critical gene influencing petal senescence in O. fragrans. Bioinformatics analysis revealed that OfERF17 lacks transmembrane transport structures but contains multiple phosphorylation sites and shares a close phylogenetic relationship with the Olea europaea var. Sylvestris. Subcellular targeting and yeast-based auto-activation tests revealed that OfERF17 resides in the nucleus and possesses a transcriptional self-activation capability. Transient expression studies conducted in O. fragrans petals indicated a decrease in the expressions of two genes associated with senescence, namely, OfSAG21 and OfACO3, when compared to the control group. Additionally, the levels of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) were markedly reduced. Transgenic Nicotiana tabacum blooms one day more than the wild type, and NtSAG12 and NtACO1 expressions were lower than wild type. These results suggest that OfERF17 functions to delay petal senescence in O. fragrans. This study enhances our knowledge of the molecular mechanisms underlying O. fragrans petal senescence and provides insights into strategies for prolonging its flowering period. Full article

Solar steam generation (SSG) has garnered significant attention for its potential in water purification applications. While composites with physically combined structures based on semiconductors or biomass have been developed for SSG, there remains a critical need for low-cost, high-efficiency devices. In this study, TiO₂ composites exhibiting excellent stability, high solar absorption, porous microstructure, and hydrophilic surfaces were identified as effective materials for SSG and water purification for the first time. A novel SSG device was designed by decorating TiO₂ onto three-dimensional carbonized Osmanthus fragrans leaves (TiO₂/carbonized OFL). Compared to directly carbonized OFL (without TiO₂) and Osmanthus fragrans leaves with templated TiO₂ (OFL-templated TiO₂), the TiO₂/carbonized OFL carbon composites demonstrated enhanced solar absorption, achieving over 99% in the visible region and more than 80% in the near-infrared region. Under solar illumination of 1 kW·m⁻², the TiO₂/carbonized OFL device achieved a high water evaporation rate of 2.31 kg·m⁻²·h⁻¹, which is 1.6 times higher than that of carbonized OFL and 3.45 times higher than OFL-templated TiO₂. Additionally, the TiO₂/carbonized OFL system exhibited remarkable efficiency in treating pharmaceutical wastewater, with a chemical oxygen demand (COD) removal efficiency of 98.9% and an ammonia nitrogen removal efficiency of 90.8% under solar radiation. Full article

Cities are major contributors to global carbon emissions; however, urban parks offer substantial potential for carbon sinks. Research on factors influencing carbon capture in urban park vegetation is still limited. This study investigates 81 urban parks in Xinyang, Henan Province, to quantify woody plant carbon storage (CS) and sequestration (CSG). By surveying all vegetation types and quantities in these parks, along with factors like park attributes, community structure, biodiversity, spatial distribution, woody plant connectivity, and spatial complexity, we create statistical models for CS and CSG. The results indicate that the average carbon storage density (CSD) in Xinyang’s urban parks is 4.01 kg/m², while the carbon sequestration density (CSGD) is 0.39 kg·C·m²·yr⁻¹. The dominant tree species are Ligustrum lucidum, Osmanthus fragrans, and Lagerstroemia indica, while species with higher carbon sequestration potential, such as Glyptostrobus pensilis, Populus deltoides, and Albizia kalkora, reveal a discrepancy between common and high-sequestration species. The study shows that park characteristics, community structure, and biodiversity are key factors impacting urban carbon sink capacity. By analyzing the relationship between these factors and carbon sinks in urban park vegetation, we create a comprehensive framework for assessing tree CS and CSG, offering quantitative support to improve carbon capture in urban parks. Full article

Due to the frequent occurrence of forest fires worldwide, which cause severe economic losses and casualties, it is essential to explore the mechanisms of forest fires. In this study, seven common broadleaf plant species from southern China were selected to observe their microscopic structural parameters. The combustion performance parameters of the leaves of these seven species were measured using a cone calorimeter, and the relationship between the microscopic structure and combustion performance was analyzed. Additionally, factor analysis was used to study the combustion intensity factor (F1), fire resistance intensity factor (F2), and the comprehensive fire risk degree (F) of the leaves of the seven plant species. Finally, regression analysis was performed between the microscopic structural parameters and the comprehensive fire risk factor. The results show the following: (1) The ratio of spongy mesophyll to palisade cells (S/P) affects the combustion performance of plant leaves. (2) The ranking of the comprehensive fire risk factor for the leaves of the seven plant species is as follows: Osmanthus fragrans var. semperflorens (OFS) > Cinnamomum camphora (CC) > Loropetalum chinense (R. Br.) Oliv. (LC) > Pterocarya stenoptera C. DC. (PS) > Loropetalum chinense var. rubrum (LCVR) > Photinia beauverdiana C. K Schneid. (PB) > Styphnolobium japonicum (L.) Schott (SJ). (3) There is a strong exponential relationship between the comprehensive fire risk factor and the microscopic structural parameters. This study is beneficial for selecting fire-resistant tree species and monitoring species with higher comprehensive fire risk. Full article

Osmanthus fragrans, a native to China, is renowned as a highly popular gardening plant. However, this plant faces significant challenges from drought stress, which can adversely affect its flowering. In this study, we found that the plasma membrane-localized gene OfPIP2 exhibited a substantial upregulation during the flowering stages and in response to drought stress. GUS staining has illustrated that the OfPIP2 promoter can drive GUS activity under drought conditions. The overexpression of OfPIP2 was found to enhance petal size by modulating epidermal cell dimensions in Petunia and tobacco. Moreover, this overexpression also bolstered drought tolerance, as evidenced by a reduction in stomatal aperture in both species. Furthermore, yeast one-hybrid (Y1H) and dual-luciferase (Dual-LUC) assays have indicated that the transcription factor OfMYB28 directly binds to the OfPIP2 promoter, thereby regulating its expression. Together, we speculated that a module of OfMYB28-OfPIP2 was not only involved in the enhancement of petal size but also conferred the improvement of drought tolerance in O. fragrans. These results contribute valuable insights into the molecular function of the OfPIP2 gene and lay a foundation for molecular breeding strategies in O. fragrans. Full article

The global warming phenomenon caused by greenhouse gas emission leads to the deterioration of the ecological environment. In urban spaces, the selection of garden tree species with high carbon sequestration rates can effectively contribute to carbon neutrality. In this study, we measured the height, diameter at breast height, and crown width of 643 ancient trees around the West Lake Scenic Spot, Hangzhou, China, and recorded their species and ages. By the biomass expansion factor method, the long-term carbon sequestration of the trees was calculated, and the corresponding statistical analysis indicated the following findings: (1) The maximum carbon sequestration of ancient trees varies with the species; the simple rational function has the best fit for the relationship between mean annual carbon sequestration and age. (2) For the five most common species in the Hangzhou area, the total individual amount of carbon sequestration per tree species can be ranked from high to low as follows: Celtis julianae, Cinnamomum camphora, Castanopsis sclerophylla, Liquidambar formosana, and Ginkgo biloba (tree age < 260 years). The ranking for trees aged above 260 years is as follows: Celtis julianae, Cinnamomum camphora, Liquidambar formosana, Castanopsis sclerophylla, and Ginkgo biloba. (3) The transient and mean annual carbon sequestration rate decreases as tree age increases; for most of the ancient trees in this research, the main growing period is 0–300 years. (4) Castanopsis sclerophylla, Liquidambar Formosana, and Osmanthus fragrans are recommended for urban landscape greening as they provide continuous long-term carbon sequestration and special landscape features. Full article

Microbial contamination remains a global issue threatening human health. In this research, silver nanoparticles (AgNPs) were fabricated using Osmanthus fragrans flower extract as a reducing agent, and biochar derived from carbonizing waste barley distillers’ grain shells was used as a support to fabricate silver-loaded carbon (C-AgNP, C-Ag). PVA-CS-C-Ag-St gel was acquired by cross-linking polyvinyl alcohol (PVA), chitosan (CS), and starch (St) with glutaraldehyde (GA). Results from SEM, FTIR, and XRD demonstrated that PVA, CS, St, and C-Ag were successfully incorporated into the gel. The PVA-CS-C-Ag-S gel showcased excellent swelling and water retention properties, which had substantial antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, with inhibition zones of 25.0 mm, 22.5 mm, and 18.0 mm, respectively. Finally, the antimicrobial analysis revealed that PVA-CS-C-Ag-St gel exhibited excellent antimicrobial properties against typical Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Overall, the PVA-CS-C-Ag-St gel holds great promise for food preservation and environmental pollution control. Full article

Carotenoids are important natural pigments that are responsible for the fruit and flower colors of many plants. The composition and content of carotenoid can greatly influence the color phenotype of plants. However, the regulatory mechanism underling the divergent behaviors of carotenoid accumulation, especially in flower, remains unclear. In this study, a new cultivar Osmanthus fragrans ‘Yanzhi Hong’ was used to study the regulation of carotenoid pigmentation in flower. Liquid chromatograph–mass spectrometer (LC-MS) analysis showed that β-carotene, phytoene, lycopene, γ-carotene, and lutein were the top five pigments enriched in the petals of ‘Yanzhi Hong’. Through transcriptome analysis, we found that the expression of the structural genes in carotenoid pathway was imbalanced: most of the structural genes responsible for lycopene biosynthesis were highly expressed throughout the flower developmental stages, while those for lycopene metabolism kept at a relatively lower level. The downregulation of LYCE, especially at the late developmental stages, suppressed the conversion from lycopene to α-carotene but promoted the accumulation of β-carotene, which had great effect on the carotenoid composition of ‘Yanzhi Hong’. Ethylene response factor (ERF), WRKY, basic helix-loop-helix (bHLH), v-myb avian myeloblastosis viral oncogene homolog (MYB), N-Acetylcysteine (NAC), auxin response factor (ARF), and other transcription factors (TFs) have participated in the flower color regulation of ‘Yanzhi Hong’, which formed co-expression networks with the structural genes and functioned in multiple links of the carotenoid pathway. The results suggested that the cyclization of lycopene is a key link in determining flower color. The modification of the related TFs will break the expression balance between the upstream and downstream genes and greatly influence the carotenoid profile in flowers, which can be further used for creating colorful plant germplasms. Full article

Osmanthus fragrans is widely used in gardening, but the short flowering period of O. fragrans affects its ornamental and economic value. ERF, as a plant ethylene response factor, is an important link in the regulation of plant senescence. In this study, we conducted a comprehensive analysis of the functional role of OfERF1-3 within the petals of O. fragrans. Specifically, the OfERF1-3 gene was cloned and subjected to rigorous sequence analysis. Subsequently, to evaluate its expression patterns and effects, gene overexpression experiments were carried out on both Nicotiana tabacum and O. fragrans. The results showed that OfERF1-3-overexpressing tobacco plants exhibited longer petal opening times compared with those of wild plants. Measurements of physiological parameters also showed that the flowers of overexpressed tobacco plants contained lower levels of malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) than those of the wild type. There was a lower expression of senescence marker genes in overexpressed tobacco and O. fragrans. A yeast two-hybrid assay showed that OfERF1-3 interacted with OfSKIP14 in a manner related to the regulation of ABA. In summary, OfERF1-3 can play a delaying role in the petal senescence process in O. fragrans, and it interacts with OfSKIP14 to indirectly affect petal senescence by regulating the ABA pathway. Full article

Scented green tea (Camellia sinensis) is a type of reprocessed green tea produced by scenting with flowers. To investigate the differences in the volatiles of scented green tea processed with four different flowers (Jasminum sambac, Osmanthus fragrans, Michelia alba, and Rosa rugosa), gas chromatography–ion mobility spectrometry (GC–IMS) and gas chromatography–mass spectrometry (GC–MS) were employed to detect and identify the volatile compounds in the four types of scented teas. GC–IMS and GC–MS identified 108 and 101 volatile compounds, respectively. The key characteristic volatile compounds, namely indole, linalool, β-myrcene, benzyl acetate, and ethyl benzoate (jasmine tea); cedrol, (E)-β-ionone, γ-decalactone, and dihydro-β-ionol (osmanthus tea); geraniol, phenylethyl alcohol, jasmone, methyl jasmonate, hexadecanoic acid, 4-ethyl-benzaldehyde, 2-methylbutyl hexanoate, and indole (michelia tea); and 3,5-dimethoxytoluene, (E)-β-ionone, and 2-methylbutyl hexanoate (rose tea), were identified through chemometric analysis combined with relative odor activity values (ROAVs) and sensory evaluation. This study provides new insights into the formation of aroma molecular fingerprints during green tea scenting with flowers, providing theoretical guidance for infusing distinct aroma characteristics into green tea during scented tea processing. Full article

The floral color phenotypes of Osmanthus fragrans cultivars range from light yellow to orange yellow, with ‘Yanzhi Hong’ being the only reported cultivar with a red color. However, the underlying reason for this unique floral coloration remains unclear. The study conducted targeted metabolomics and transcriptomics analyses on the petals of ‘Yanzhi Hong’ at both initial and peak flowering stages. Candidate gene expression was validated, and expression levels of the petals of three cultivars were compared using RT-qPCR. The results revealed the presence of 27 components in the petals of ‘Yanzhi Hong’, including 5 carotenoids, 8 xanthophylls, and 14 xanthophyll esters. Notably, lycopene was detected in abundance for the first time in O. fragrans cultivars. Carotenes accounted for 78.82 ± 3.17% and 91.19 ± 1.69% of the total carotenoid content in petals during the initial and peak flowering stages, respectively, with all carotene contents increasing during the peak flowering period. β-carotene, lycopene, and γ-carotene were identified as the top three carotene components in petals during both initial and full flowering stages. The unique blush red color of ‘Yanzhi Hong’ petals could be attributed to the low content of α-carotene and the rich accumulation of lycopene. Furthermore, a total of 1550 differentially expressed genes (DEGs) were identified in petals at the peak flowering stage relative to the initial flowering stage, with 1003 genes being downregulated and 547 genes being upregulated during the full flowering stage. There are 926 differentially expressed genes (DEGs) annotated in the Gene Ontology (GO) database. Among these DEGs, those that were downregulated and upregulated during the peak flowering period showed significant enrichment in carbohydrate metabolism and oxidation–reduction processes, respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified 14 structural genes associated with phenylpropanoid biosynthesis and 7 structural genes linked to carotenoid biosynthesis. Expression levels of candidate genes involved in carotenoid biosynthesis were examined in the petals of three cultivars (‘Yanzhi Hong’, ‘Liuye Jingui’, and ‘Gecheng Dangui’) at both the initial and peak flowering stages. The results indicated that the decreased expression of LYG009054 (LYCE) and LYG018651 (LYCB) in ‘Yanzhi Hong’ resulted in higher lycopene accumulation and lower α-carotene content in the petals. This study offers valuable insights into the mechanisms underlying the unique flower color phenotype of O. fragrans, proving a basis for further research on carotenoid metabolism pathways and the breeding of new cultivars with a variety of flower colors in O. fragrans. Full article

Ethylene-Responsive Factor (ERF) is a key element found in the middle and lower reaches of the ethylene signal transduction pathway. It is widely distributed in plants and plays important roles in plant growth and development, hormone signal transduction, and various stress processes. Although there is research on AP/ERF family members, research on AP2/ERF in Osmanthus fragrans is lacking. Thus, in this work, AP2/ERF in O. fragrans was extensively and comprehensively analyzed. A total of 298 genes encoding OfAP2/ERF proteins with complete AP2/ERF domains were identified. Based on the number of AP2/ERF domains and the similarity among amino acid sequences between AP2/ERF proteins from A. thaliana and O. fragrans, the 298 putative OfAP2/ERF proteins were divided into four different families, including AP2 (45), ERF (247), RAV (5), and SOLOIST (1). In addition, the exon–intron structure characteristics of these putative OfAP2/ERF genes and the conserved protein motifs of their encoded OfAP2/ERF proteins were analyzed, and the results were found to be consistent with those of the population classification. A tissue-specific analysis showed the spatiotemporal expression of OfAP2/ERF in the stems and leaves of O. fragrans at different developmental stages. Specifically, 21 genes were not expressed in any tissue, while high levels of expression were found for 25 OfAP2/ERF genes in several tissues, 60 genes in the roots, 34 genes in the stems, 37 genes in young leaves, 34 genes in old leaves, 32 genes in the early flowering stage, 18 genes in the full flowering stage, and 37 genes in the late flowering stage. Quantitative RT-PCR experiments showed that OfERF110a and OfERF110b had the highest expression levels at the full-bloom stage (S4), and this gradually decreased with the senescence of petals. The expression of OfERF119c decreased first and then increased, while the expression levels of OfERF4c and OfERF5a increased constantly. This indicated that these genes may play roles in flower senescence and the ethylene response. In the subsequent subcellular localization experiments, we found that ERF1-4 was localized in the nucleus, indicating that it was expressed in the nucleus. In yeast self-activation experiments, we found that OfERF112, OfERF228, and OfERF23 had self-activation activity. Overall, these results suggest that OfERFs may have the function of regulating petal senescence in O. fragrans. Full article

Fire is a prevalent hazard that poses a significant risk to public safety and societal progress. The continuous expansion of densely populated urban areas, exacerbated by global warming and the increasing intensification of urban heat islands, has led to a notable increase in the frequency and severity of fires worldwide. Incorporating measures to withstand different types of calamities has always been a crucial aspect of urban infrastructure. Well-designed plant communities play a pivotal role as a component of green space systems in addressing climate-related challenges, effectively mitigating the occurrence and spread of fires. This study conducted field research on 21 sites in the green belt around Shanghai, China, quantifying tree morphological indexes and coordinate positions. The spatial structure attributes of different plant communities were analyzed by principal component analysis, CRITIC weighting approach, and stepwise regression analysis to build a comprehensive fire resistance prediction model. Through this research, the relationship between community spatial structures and fire resistance was explored. A systematic construction of a prediction model based on community spatial structures for fire resistance was undertaken, and the fire resistance performance could be quickly judged by easily measured tree morphological indexes, providing valuable insights for the dynamic prediction of fire resistance. According to the evaluation and ranking conducted by the prediction model, the Celtis sinensis, Sapindus saponaria, Osmanthus fragrans, Koelreuteria paniculata, and Distylium racemosum + Populus euramericana ‘I-214’ communities exhibited a high level of fire resistance. On the other hand, the Koelreuteria bipinnata + Ligustrum lucidum, Ginkgo biloba + Camphora officinarum + Ligustrum lucidum, and Ligustrum lucidum + Sapindus saponaria communities obtained lower scores and were positioned lower in the ranking. It is emphasized that the integration of monitoring and regulation is essential to ensure the ecological integrity and well-being of green areas in the Wildland–Urban Interface. Full article