Search Results (97)

Background and Objectives: In recent years, there has been growing interest in the production of ingredients rich in dietary fiber and antioxidants, such as green banana flours. This study evaluated the effect of consumption of mixed green banana pulp (PF) and peel (PeF) flours on the body weight gain, adiposity, lipid profile, and intestinal morphology of Wistar rats. Methods: Male young rats were divided into four groups (n = 8) that received a standard diet (SD), or one of the following three test diets: M1 (SD + 90% PF/10% PeF), M2 (SD + 80% PF/20% PeF), or P (SD + 100% PF) for 28 days. Results: Rats from M1, M2, and P groups showed reduced body weight gain and adiposity and had lower contents of total cholesterol, LDL-c, VLDL-c, and triglycerides. Animals from M1 and M2 groups had an increase in cecum weight, fecal moisture, acetic acid concentration, and crypt depth and reduced fecal pH. Moreover, consumption of the M1, M2, and P diets increased the expression of proteins involved in intestinal functionality. Significant negative correlations were observed between consumption of resistant starch and soluble dietary fiber, from the flours, and weight gain (r = −0.538 and r = −0.538, respectively), body adiposity (r = −0.780 and r = −0.767, respectively), total cholesterol (r = −0.789 and r = −0.800, respectively), and triglycerides (r = −0.790 and r = −0.786, respectively). Conclusions: Mixed green banana pulp and peel flour proved to be a viable alternative as a food ingredient that can promote weight loss, improve lipid profile and intestinal morphology, and minimize post-harvest losses. Full article

We intend to prepare pumpkin puree based on the health benefits of both the flesh of pumpkins (Cucurbita maxima) and the peel of bananas (Musa spp.). However, before we begin we would like to optimize the conditions by using thermosonication, rather than conventional pasteurization, and a quantity of banana peel powder. Therefore, this study aimed to use response surface methodology (RSM) to find the best temperature and time settings for the ultrasonication process of functional pumpkin puree (FPP) with banana peel powder (BPP) to increase the amount of total phenolics and DPPH scavenging activity while also making the quality of the puree better. To enhance the FPP production process, quality attributes (responses), including total phenolic content (TPC), 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity, pH, acidity, and color change (ΔE) were assessed. The model demonstrated validity (R² = 0.97–0.988) and was highly significant (p < 0.0001). The experimental values of the responses supported the validity of the utilized RSM model, which closely matched the expected values at the ideal processing conditions of process temperature (40 °C), ultrasonic process duration (8.23 min), and BPP (2 g/100 g). Under these conditions, the generated FPP had quality attributes of 205.79 mg GAE/100 mL, 83.01%, 5.78, 0.32 g/100 g, and 3.81 for responses, respectively. Full article

Decentralized sanitation in rural areas urgently requires accessible and nature-based solutions to achieve Sustainable Development Goal 6 (clean water and sanitation for all). However, monitoring studies of such ecotechnologies in disperse communities remain limited. This study evaluated the performance of an evapotranspiration tank (TEvap), designed with community participation, for the treatment of domestic sewage in a rural Quilombola household in the Brazilian Cerrado. The system (total area of 8.1 m², with about 1.0 m² per inhabitant) was monitored for 218 days, covering the rainy season and the plants’ establishment phase. After 51 days, the TEvap reached operational equilibrium, maintaining a zero-discharge regime, and after 218 days, 92.3% of the total system inlet volumes (i.e., 37.47 in 40.58 m³) were removed through evapotranspiration and uptake by cultivated plants (Musa spp.). Statistical analyses revealed correlations that were moderate to strong, and weak between the blackwater level and relative humidity (Pearson correlation coefficient, r = 0.75), temperature (r = −0.66), and per capita blackwater contribution (r = 0.28), highlighting the influence of climatic conditions on system efficiency. These results confirm the TEvap as a promising, low-maintenance, and climate-resilient technology for decentralized domestic sewage treatment in vulnerable rural communities, with the potential to support sanitation policy goals and promote public health. Full article

Bananas and plantains, belonging to the Musa genus, are important food crops that sustain the livelihoods of countless smallholder farmers globally. However, their production is hindered by various challenges, including abiotic and biotic stresses, climate change, and poor access to clean planting materials, which negatively impact their yields. Addressing these constraints is essential for improving production and ensuring food security. This study investigated the influence of triploid genome background and exogenous growth regulators on the regeneration of Musa cultivars [Gros Michel (AAA genome), Obino l’Ewai and Silk (AAB genome), and Poteau Naine (ABB genome)]. Shoot tip explants of the AAA, AAB, and ABB triploid genomes were cultured in Murashige and Skoog (MS) media supplemented with varying 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), or naphthaleneacetic acid (NAA) hormones. Shoot induction was successfully achieved within 21.50 ± 2.00 days, with AAA exhibiting the highest shoot induction frequencies ranging from 30.00 ± 1.57% to 100% and shoot numbers per explant ranging from 3.00 ± 0.50 to 8.80 ± 0.80, followed by the ABB genome ranging from 20.00 ± 3.45% to 100% and from 2.00 ± 0.55 to 5.60 ± 0.50 shoots, and the AAB genome ranging from 17.50 ± 5.01% to 100% and from 2.00 ± 0.04 to 6.60 ± 0.25 shoots, respectively, in media amended with 1.2 to 6.0 mg.L⁻¹ BAP and 0.1 mg.L⁻¹ IAA. The highest rooting rate of 100% was recorded in all three genomes in media containing 1.4 mg.L⁻¹ IBA and 0.5 mg.L⁻¹ IAA, with the AAA genome producing the maximum number of 14.8 roots per explant. The results indicate the positive influence of the AAA genome background on in vitro regeneration and its potential utilization for genomic editing transformation protocols Full article

Small non-coding microRNAs (miRNAs) play crucial roles in the degradation of the messenger RNAs (mRNAs) that are involved in various biological processes post-transcriptionally and translationally. Many plants, especially Musa spp. (plantains and bananas), which are important perennial herbs of the family Musaceae, experience significant yield loss due to abiotic stressors, yet only a few miRNAs involved in this response have been identified. This study employed in silico analyses of transcriptome shotgun assembly (TSA) and expressed sequence tag (EST) sequences to identify Musa miRNAs and their target genes. Leaf and root tissues from three Musa genomic groups (AAA, AAB, and ABB) under drought stress were analyzed using quantitative real-time PCR (qRT-PCR) to validate the expression of miRNAs. A total of 17 potential conserved miRNAs from 11 families were identified, with the minimal folding free energies (-kcal/mol) of precursors ranging from −136.00 to −55.70, as observed through RNA folding analysis. Six miRNAs (miR530-5p, miR528-5p, miR482a, miR397a, miR160h, and miR399a) showed distinct tissue-specific expression patterns in the roots and leaves across the three groups. A total of 59 target regulatory transcription factors and enzymes involved in stress response, growth, and metabolism were predicted. Of these, 11 targets were validated for miR530-5p, miR528-5p, miR482a, and miR397a, using qRT-PCR. These four stress-responsive miRNAs exhibited an inverse expression relationship with their target genes across two different tissues in Musa groups. This research provides insights into miRNA-mediated drought stress responsiveness in Musa spp., potentially benefiting future studies on gene regulation under drought stress. Full article

The genetic diversity of banana plants (Musa spp.) can result in different phosphorus requirements, highlighting the importance of studies performed to optimize phosphate fertilization in order to improve the productivity and sustainability of banana plantations. This study assessed the effects of phosphate fertilization on the duration of the harvest season, bunch mass, soil fertility and foliar nutrition of BRS SCS Belluna banana plants. A replicated trial was performed in two consecutive harvests, with different phosphorus levels, i.e., 25, 50, 75, 100, 125 and 150% of the recommended level for the crop. Soil analyses included macro- and micronutrients, silicon, acidity, organic matter, cation exchange capacity and base saturation. Leaf tissue was analyzed for mineral content. Thermophosphate had different effects on soil fertility and leaf nutrients. Calcium and phosphorus in the soil increased linearly. In the leaf, a reduction in zinc content was mainly observed. The lower temperatures and accumulated rainfall that occurred during the second harvest season are related to a greater number of days between flowering and harvest and a lower bunch mass. These results could support fertilization programs aimed at ensuring the long-term sustainability of phosphorus nutrition in banana plantations. Full article

The genus Musa L. is one of the most important genera worldwide due to its use in food as a source of carbohydrates. A morphological characterization was performed to evaluate the potential of 100 accessions of Musa spp. from the Amazon region of Ecuador, applying 73 qualitative and quantitative descriptors in addition to the ecogeographic characterization. The multivariate analyses identified four large groups: The first is composed of the Musa AAB Simmonds ecotype “Hartón Plantain” and the “Cuerno Clone”. The second group is composed of the Musa acuminata Colla ecotype “Orito”. The third group is composed of the Musa acuminata ecotype “Malay plantain or red plantain”; and the fourth group is composed of the Musa × paradisiaca L. AAB ecotype “Barraganete” and banana or banana materials and the Musa AAB Simmonds ecotype “Plátano Dominico”. The qualitative descriptors with the highest discriminant value were the shape of the ♂ floret bud, the appearance of the rachis, and the pigmentation of the compound tepal, and the quantitative discriminant characters were the height of the pseudostem, the length of the leaf blade, the width of the leaf blade, and the weight of the raceme. The analysis with CAPFITOGEN of these 100 accessions through the ecogeographic characterization map identified 23 categories, highlighting category 20 with a coverage of 40.35%, which mainly includes the provinces of Orellana, Sucumbíos, part of Napo, Pastaza, and Morona Santiago. This category occurs within an annual temperature range between 21.6 °C and 27 °C, an apparent density of 1.25 to 1.44 g cm⁻³, and a cation exchange capacity (CEC) of 4 to 29 Cmol kg⁻¹. The morphological characterization of 100 Musa accessions revealed significant phenotypic variability, with four distinct morphological groups identified through cluster analysis. Key differences were observed in traits such as bunch weight, fruit length, and vegetative vigor. This variability highlights the potential of certain accessions for use in genetic improvement programs. The findings contribute valuable information for the efficient conservation, selection, and utilization of the Musa germplasm in Ecuadorian agroecosystems. The results demonstrate the existence of an important genetic variability in the INIAP Musa Germplasm Bank in the Ecuadorian Amazon region. Full article

Fusarium oxysporum f. sp. cubense (Foc) causes Fusarium wilt, a devastating epidemic disease that has caused widespread damage to banana crops worldwide. We report the draft genomes of Foc race 1 (16117) and Foc tropical race 4 (Fusarium odoratissimum) (CNSD1) isolates from China, assembled using PacBio HiFi sequencing reads, with functional annotation performed. The strains group in distinct lineages within the Fusarium oxysporum species complex. This genetic resource will contribute towards understanding the pathogenicity and evolutionary dynamics of Foc populations in banana-growing regions around the world. Full article

Banana inflorescences are usually discarded, but there has been interest in managing this by-product to turn it into a product with added value. Herein, the inflorescences of seven cultivars were processed into flour and evaluated for their physicochemical characteristics. The weight of the inflorescences ranged from 681.3 to 1245.4 g, with bracts accounting for more than 40%. The Prata Anã cultivar had the largest inflorescence. The part of the inflorescence was the main factor differentiating the flours, with the effect of the cultivar dependent on the part processed. All flours had high levels of fiber (27.70–41.91 g/100 g) and carbohydrates (19.30–33.96 g/100 g). The palm flours were differentiated by their higher levels of protein (17.4–19.4 g/100 g), and the flower flours by their higher levels of lipids (5.89–7.97 g/100 g). The bract flours had a higher water holding capacity (5.62–6.78%) and browning index (40.7–42). The bract and flower flours were less dissimilar. Results revealed the high nutritional quality of the flours and the prospect of using them as a non-conventional food source. Understanding the differences between banana inflorescence flours expands their possible uses and promotes sustainable agricultural production in terms of efficient banana by-product management. Full article

The objective of this study was to develop meat analog burgers using green banana biomass (GBB) combined with teff and chickpea derivatives, as well as to evaluate their chemical and technological quality, in addition to comparing them with industrialized meat and plant-based burgers. Four formulations (F1: 100% GBB; F2: 75% GBB; F3: 60% GBB; and F4: 50% GBB, with 25% teff and 25% chickpeas) were developed and compared to the following industrialized burgers: F5 (meat-based) and F6 (plant-based). All the samples were subjected to physical (initial/final weight, diameter, height, color, and texture) and chemical (pH, proximate composition, fiber, and caloric value) analyses. Among the treatments, F4 showed the highest initial weight (223.00 g) and final weight (201.66 g), initial diameter (12.33 cm) and final diameter (11.96 cm), and a reduction in height from 2.04 cm to 1.57 cm. In terms of firmness, F4 was significantly higher than that of the other treatments (p ≤ 0.05). Regarding its chemical composition, F4 presented a high protein content (10.25%) and energy value (285.30 kcal). The total fiber content for F1 to F4 was 3.62%, 3.74%, 3.97%, and 4.15%, respectively, while F6 (plant-based) reached 5.69%. These findings indicate that the combination of GBB with teff and chickpeas, especially in F4, was favorable for producing meat analog burgers with promising technological and nutritional properties. Full article

Gastrointestinal parasites have been reported to have negative effects on chickens reared in rural areas in African countries. Hence, smallholder farmers use ethnoveterinary remedies to control these parasites in village chickens. This study used online databases (ScienceDirect, PubMed, and Google Scholar) to search, gather, and review articles published between January 1990 and June 2024 where ethnoveterinary intervention was considered to control gastrointestinal parasites, following PRISMA guidelines. A total of 540 articles were generated, and only 17 articles passed the screening process and were considered for further analysis. The findings of this review indicate that ethnoveterinary remedies are used by smallholder farmers to control gastrointestinal parasites such as Ascaridia galli, Capillaria spp., Choanotaenia infundibulum, Eimeria spp., Heterakis gallinarum, and Raillietina cesticillus in village chickens. Aloe spp., Carica papaya, Musa paradisiaca, and Venonia amygdalina were reported as the most-preferred medicines, and farmers utilized different plant parts ranging from leaves (48.8%), bark and roots (12.2%), tubers (7.3%), and seeds (4.9%). These remedies are administered per os, topically, and dermally. The current review successfully summarized ethnoveterinary intervention used by smallholder farmers to control gastrointestinal parasites in chickens found in African countries. Therefore, there is a need to investigate the efficacy of these identified ethnoveterinary medicines against gastrointestinal parasites in chickens. Full article

The banana inflorescence (BI) is a rich source of antioxidants and dietary fiber, making it a valuable by-product of banana harvesting. This study aimed to maximize the ultrasound-assisted extraction (UAE) of antioxidant compounds from BI and analyze the residue’s composition and functional properties. The Box–Behnken Design was applied to test different ultrasound powers, temperatures, and sample-to-solvent ratios on the total phenolic content (TPC) and total flavonoid content (TFC). The UAE conducted at 350 W, 55 °C, 1:30 (g/mL) ratio for 30 min resulted in the highest TPC (1637.12 mg/100 g) and TFC (22.97 mg/100 g). Isovanillin, caffeine, naringin, sinapaldehyde, nicotinic acid, quinic acid, malic acid, and fumaric acids were reported for the first time for BI. The extract obtained by UAE showed a higher content of these compounds and higher antioxidant activity than that obtained through conventional extraction (orbital shaking). The residue obtained after UAE presented dietary fiber as the main component (71.91 g/100 g) and a higher oil absorption index (5.78 g/g) than untreated BI. Therefore, BI is a source of bioactive compounds, and both the extract and residue can be used in the pharmaceutical, food, and cosmetic industries, enabling the productive sector to move closer to the circular economy. Full article

Lemongrass (Cymbopogon citratus) has potential for intercropping with banana (Musa spp.) plants, thus contributing to the sustainability of plantations. The study evaluated the growth and yield of ‘Prata Anã’ banana and seasonality, yield and essential oil properties of lemongrass grown in intercropping and the land equivalent ratio. A randomized block design in a split plot arrangement was used, evaluating two cropping systems (sole crop and intercropping) and three harvest seasons during the year for lemongrass and two harvest seasons for banana, separately. The banana can be intercropped with lemongrass without interfering with its growth and production. The biomass production and essential oil yield of lemongrass differed according to cropping system and seasonality. The chemical composition of the essential oil showed stability in the concentration of citral (mixture of neral and geranial), with an average of 94.43%. Seasonal variations were observed in the content of these compounds and other components of the essential oil, emphasizing the importance of the time of harvest in the overall value of the oil. The result demonstrates the viability of intercropping, which not only provides crop yields for both species and produces more per unit area than a sole crop but also optimizes the use of resources and promotes more sustainable farming practices. Full article

Banana (Musa spp.) is susceptible to low-temperature stress and other environmental stresses, which can hinder the growth and development. Succinic semialdehyde dehydrogenase (SSADH) is critical for GABA biosynthesis and plays a crucial role in plants. However, the SSADH genes of bananas have not been studied. This study found 19 MaSSADHs, 18 MbSSADHs, and 18 MiSSADHs from the banana genome. According to the phylogenetic tree, these genes can be categorized into five branches. This study cloned the MaSSADH1-14 from banana. The subcellular localization assays of MaSSADH1-14 in tobacco leaves confirmed that the presence of SSADH was not only localized mitochondrion but also localized chloroplast. The cis-elements of the SSADH gene family are related to the potential regulation of the banana SSADH gene family; their involvement in diverse stress responses. Transcriptomic data was utilized to examine the effect of MaSSADH genes under cold stress in bananas. The results of RT-qPCR were consistent with transcriptome data. These results showed that most MaSSADHs are passively responsive to low-temperature treatment. In addition, transient overexpression of MaSSADH1-14 in Nicotiana benthamiana leaves resulted in the content of GABA increasing, indicating that MaSSADH1-14 may be involved in the accumulation of GABA of banana. Collectively, these results improve knowledge of the SSADH gene family in banana and establish a basis for comprehending its biological roles in response to low temperatures. Full article

The regulatory mechanisms underlying embryogenic callus (EC) formation in polyploid bananas remain unexplored, posing challenges for genetic transformation and biotechnological applications. Here, we conducted transcriptome sequencing on cultured explants, non-embryogenic callus, EC, and browning callus in the ABB cultivar ‘MJ’ (Musa spp. cv. Bengal). Our analysis of differentially expressed genes (DEGs) revealed significant enrichment in plant hormones, MAPK, and zeatin biosynthesis pathways. Notably, most genes in the MJ variety exhibited balanced expression of the A and B alleles, but A-specific allele expression was dominant in the key signaling pathways, whereas B-specific allele expression was very rare during EC induction. In the auxin signaling pathway, six A-specific MJARF genes were markedly downregulated, underscoring their critical roles in the negative regulation of callus formation. Additionally, six A-specific MJEIN3 alleles were found to play negative regulatory roles in ethylene signaling during EC development. We also identified phenylpropanoids responsible for enzymatic browning. Furthermore, the expression patterns of transcription factors in bananas exhibited specific expression modes, highlighting the unique mechanisms of callus formation. This study enhanced our understanding of the regulatory roles of these alleles in EC induction and offers new insights into the utilization of alleles to improve the efficiency of somatic embryogenesis in bananas. Full article