Search Results (2,925)

Aquaponics is an integrated and resource-efficient production system that combines aquaculture and hydroponics in a closed-loop environment with reduced water consumption and nutrient losses. The present study evaluated the nutritional composition, mineral profile, microbiological quality, and antioxidant-related phytochemical characteristics of red amaranth (Amaranthus cruentus L.) cultivated in a recirculating aquaponic system under controlled environmental conditions. Leaf biomass was analyzed for proximate composition, macro- and micronutrient content, total phenolic and flavonoid compounds, betalains, chlorophyll pigments, and antioxidant activity using standard analytical and spectrophotometric methods. The results demonstrated high crude protein content and substantial accumulation of essential minerals, particularly calcium, potassium, and magnesium. The analyzed biomass also exhibited elevated levels of phenolic compounds, flavonoids, betalains, and chlorophyll pigments associated with considerable antioxidant potential. The pigment profile suggested good physiological adaptation of plants to aquaponic cultivation conditions. In addition, microbiological analysis confirmed acceptable hygienic quality and safety of the harvested plant material. Overall, the findings indicate that red amaranth can be successfully cultivated in aquaponic systems while maintaining high nutritional value and functional food potential. The study highlights aquaponic cultivation as a sustainable approach to producing nutrient-dense leafy vegetables within environmentally responsible agricultural systems. Full article

Aims: This study aimed to evaluate the agreement of a frequency sampling method (FR) as a tool for species identification while measuring undisturbed sward height. Methods: The botanical composition of both grazing systems was evaluated during the pre-grazing and post-grazing periods using two methods: the Dry Weight Rank (DWR) and FR. A non-parametric Friedman test was applied to compare evaluation methods and grazing moments. Differences in detection frequencies between methods were assessed using McNemar’s test for paired binary data. Results: The evaluation method did not influence the relative abundance of the three most abundant plant species identified: U. decumbens, Paspalum genus, and Commelinaceae weeds. A high positive Lin’s concordance correlation coefficient (CCC) was observed between the two methods in U. decumbens, Paspalum genus, U. brizantha cv. Marandú, U. plantaginea, U. arrecta, and U. humidicola (CCC ≥ 0.70). We observed lower agreement for some functional groups, particularly Commelinaceae weeds (CCC = 0.38), narrow-leaf weeds (CCC = 0.46), and Cyperaceae weeds (CCC = 0.17). Canonical correlation analysis (CCA) between the chemical composition of leaves and the botanical composition estimated by the DWR revealed two significant canonical functions (p < 0.01), with canonical correlations of 0.692 and 0.478 for the first and second functions, respectively. When botanical composition estimated by the FR was used as a regressor for leaf chemical composition, three significant canonical functions (p < 0.01) were identified, with canonical correlations of 0.632, 0.529, and 0.425 for the first, second, and third functions, respectively. Conclusions: FR represents a practical and complementary approach for assessing botanical composition and plant diversity in heterogeneous tropical grasslands, particularly for the rapid monitoring of dominant species. However, lower agreement was observed for some low-abundance functional groups, indicating reduced FR sensitivity for certain plant types. Full article

Background: Several studies reveal an inverse relation between serum 25-hydroxyvitamin D [25(OH)D] concentration and the risk of acute ischemic stroke (AIS). The aim of this study was to determine relationships between 25(OH)D concentration and the course and outcomes of AIS treatment and the level of indices of inflammatory response to brain injury. Patients and Methods: Retrospective analysis of medical documentation of 1381 real-world AIS patients hospitalized in a single center between 1 January 2020 and 31 May 2025. Serum 25(OH)D level, several inflammatory indices, and clinical data were assessed. Results: Compared to patients in the lowest quartile of 25(OH)D concentration, those in the highest quartile had a shorter length of in-hospital stay, a lower risk of all-cause death, and a lower score for disability on a modified Rankin scale (mRS). Along with an increase in 25(OH)D quartiles, we found: a decrease in neutrophil count; a decrease in glucose, HbA1c, albumin, C-reactive protein (CRP), and CRP-to-albumin, -lymphocyte, -neutrophil, and -platelet ratios; lower neutrophil-to-lymphocyte and -albumin ratios, and lower systemic immune inflammation, and systemic inflammation response indices. In multifactorial logistic regression, the quartile of 25(OH)D (OR, 95% CI: 1.52, 1.09–2.12; p = 0.012) was the only variable to have a positive association with a mRS score ≤ 2 at discharge from hospital, and neutrophil-to-lymphocyte ratio, age, diabetes, and treatment with endovascular mechanical thrombectomy were biomarkers of poor functional status at discharge. Conclusions: Higher 25(OH)D concentration in AIS patients is related to better survival and a lower level of inflammatory response indices and disability at discharge. Full article

In the present work, we study a class of nonhomogeneous linear systems with fractional derivatives in Caputo’s sense of incommensurate order and distributed delays, satisfying the Perron condition. More precisely we study the impact of the Perron condition on the boundedness of the fundamental and extended fundamental matrices of the corresponding homogeneous system. We prove that if the nonhomogeneous system satisfies the Perron condition, then the fundamental matrix $C(t,s)$ and the extended fundamental matrix $Q(t,s)$ of the homogeneous system are uniformly bounded. As a consequence we obtain that the boundedness of the matrix $Q(t,s)$ is a necessary and sufficient condition for the uniform stability of the zero solution of the homogeneous system. Furthermore, we also prove that the extended fundamental matrix $Q(t,s)$ tends to zero when $t\to \infty$, which is a necessary and sufficient condition for the uniform asymptotic stability of the zero solution of the homogeneous system under study. Full article

The effects of red seaweed, psyllium husk, and chia seeds on the structural and functional properties of comminuted meat batters were evaluated. Meat batters were formulated with 1% of each ingredient or their combinations totaling 1% and evaluated for pH, cooking loss, microstructure, texture profile analysis, color, rheology, and protein interactions. Formulation did not affect (p ≥ 0.08) pH or cooking loss, indicating that water- and lipid-holding capacity and emulsion stability were preserved across treatments. Hardness increased (p ≤ 0.05) in treatments containing red seaweed, alone or combined with psyllium husk. Fiber addition did not influence (p ≥ 0.17) raw batter color; however, cooked products showed differences (p ≤ 0.05) in lightness (L*) and total color change (ΔE*). Rheological analysis indicated similar viscoelastic behavior among treatments with no significant differences among treatments (p ≥ 0.07) for storage modulus, loss modulus, or tangent delta at the start, peak, or end of the small-amplitude oscillatory shear test. Microstructural observations revealed treatment-dependent networks, and protein solubility analysis showed changes (p ≤ 0.05) in ionic and hydrogen bonding, while disulfide bonds were unaffected (p = 0.60). Incorporation of 1% of these ingredients maintained desirable physicochemical, textural, and functional properties, highlighting their potential as ingredients in meat batters. Full article

Pregnancy involves profound metabolic, hormonal, and immunological adaptations essential for fetal development; however, disturbances may lead to complications such as preeclampsia (PE), pre-gestational diabetes, and gestational diabetes mellitus (GDM). These conditions are closely linked to oxidative stress, inflammation, endothelial dysfunction, impaired placentation, and metabolic dysregulation. Consequently, dietary strategies capable of modulating these pathways are of increasing interest. Edible and medicinal mushrooms are widely studied as functional food due to the content of bioactive compounds with antioxidant, anti-inflammatory, immunomodulatory, and metabolic regulatory effects. This review summarizes the nutritional composition of mushrooms and highlights key bioactive constituents with antioxidant and metabolic regulatory properties. Among them, ergothioneine has emerged as a key molecule due to its potent redox-buffering capacity and its potential involvement in the activation of the Nrf2 signaling pathway, a master regulator of cellular antioxidant defense. Through modulation of Nrf2-dependent gene expression, mushroom-derived compounds may contribute to improved cellular resilience against oxidative damage relevant to PE and GDM pathophysiology. Mushroom consumption has additionally been associated with improved glycemic control and enhanced antioxidant defenses in experimental and limited clinical studies, although evidence regarding the prevention or management of hypertensive and metabolic pregnancy complications remains insufficient. Although preclinical findings are promising, clinical evidence remains limited. Further well-designed prospective studies and randomized controlled trials are required to determine efficacy, safety, optimal intake, and active compounds responsible for these effects. Nevertheless, current evidence supports the biological plausibility that edible and medicinal mushrooms are promising dietary modulators of the ergothioneine–Nrf2 axis with potential relevance for maternal–fetal health. Full article

This study investigates subcritical water extraction (SWE) as an alternative to hydroalcoholic extraction for the production of Echinacea purpurea root extracts standardized to hydroxycinnamic acids (cichoric and caftaric acids). Extractions were performed at 100 °C, 125 °C, 150 °C, and 170 °C for 10–30 min. The recovery of cichoric and caftaric acids was significantly (p < 0.05) influenced by extraction temperature, with the highest values obtained within the range of 100–125 °C. Further experiments identified 110 °C for 10 min as the optimal condition, yielding the highest cumulative recovery of cichoric and caftaric acids (1.87 ± 0.10% of dry material). In the resulting dry extracts, SWE at 100–125 °C produced hydroxycinnamic acid contents of 5.5–7.1%, whereas the total dry extract yield in-creased from 24–28% at 100 °C to 40–41% at 150–170 °C (p < 0.05). Higher temperatures, however, reduced cichoric and caftaric acid cumulative content to 0.6–1.7% (p < 0.05), indicating a degradation of the target compounds. In contrast, total polyphenol recovery in-creased continuously with temperature, reaching 4.86% at 170 °C for 30 min. This was accompanied by marked increases in rutin, gallic and caffeic acid, reaching 458.5 mg/100 g dry weight (DW), 175.5 mg/100 g DW and 945.7 mg/100 g DW (p < 0.05), respectively, suggesting the release of bound phenolics following partial disruption of plant cell wall structures. SWE also enhanced the extraction of carbohydrates, uronic acids, fructans, proteins and organic acids, demonstrating an extensive temperature-dependent modification of the root matrix. 5-HMF was not detected in extracts obtained below 125 °C, but increased progressively at higher temperatures, reaching 200 mg/100 g (p < 0.05) at 170 °C. Biological evaluation in the human colorectal adenocarcinoma cell line (HT29) showed favorable cytocompatibility of SWE extracts, confirmed by cell viability, morphological assessment and low DNA damage in the Comet Assay. Overall, SWE enables the production of cichoric and caftaric acid-standardized E. purpurea extracts without organic solvents, supporting its application in pharmaceutical, nutraceutical, food and cosmeceutical products. Full article

Background/Objectives. The intestinal microbiota is a key contributor to postoperative complications, yet direct interventions targeting dysbiosis—antibiotics, probiotics, and synbiotics—have produced inconsistent results. This paradox indicates a fundamental gap in understanding host–microbiota interactions under surgical stress. We aimed to re-examine the causal role of dysbiosis in postoperative pathogenesis and propose a revised therapeutic paradigm centered on host barrier protection. Methods. A narrative literature review was conducted, searching PubMed/MEDLINE, Scopus, and Web of Science for articles published between 2009 and 2025. Reference lists of included publications were additionally screened. Studies in English and Russian were eligible; 107 references were included. Results. We hypothesize that dysbiosis in surgical patients may, at least in part, represent a predictable ecological response to systemic hypoperfusion, pharmacological burden, and ischemia–reperfusion injury, rather than acting solely as an independent pathogenic agent. Microbial shifts, characterized by the depletion of short-chain fatty acid-producing commensals and the expansion of pathobionts, frequently accompany epithelial injury; however, available human data are predominantly observational and do not permit definitive determination of the temporal sequence. This hypothesis provides the conceptual foundation for the proposed therapeutic reorientation. Conclusions. The present findings support the rationale for transitioning from microbiome manipulation to a “host-first” strategy, which prioritizes the restoration of intestinal barrier integrity through the administration of cytoprotective agents and targeted metabolic substrates (glutamine and butyrate). We propose the Gut Resilience Index (GRI) as a theoretical construct to identify patients approaching a critical threshold necessitating rescue therapy. It must be emphasized that both the “host-first” strategy and the GRI remain hypothetical frameworks requiring prospective validation. The most critical next steps include the development and validation of the GRI in prospective cohort studies, as well as randomized controlled trials directly comparing barrier-oriented strategies with standard care. Full article

Antibiotic resistance has emerged as a major global health challenge, underscoring the urgent need for alternative therapeutic strategies capable of enhancing the efficacy of existing antibiotics. In this context, saponin-based nanomaterials have attracted considerable attention due to their potential as antibiotic-modulating systems. This study investigated a saponin-enriched fraction obtained from the bark of Sarcomphalus joazeiro Mart. (SEF-4), its application in the green synthesis of silver nanoparticles, and the antibiotic-modulating potential of the resulting nanoformulation. SEF-4 was obtained from the ethanolic bark extract through liquid–liquid partitioning (52% yield), followed by column chromatographic purification and chemical characterization using LC-ESI-QTOF-MS. The purified fraction was subsequently employed as both a reducing and stabilizing agent for the synthesis of silver nanoparticles (putative AgNP-SEF-4), which were physicochemically characterized. Antibacterial activity and antibiotic-modulating effects were evaluated using the broth microdilution method against standard and multidrug-resistant bacterial strains. LC-ESI-QTOF-MS analysis enabled the putative identification of five jujubogenin-type triterpenoid saponins bearing tetra-, penta-, and hexasaccharide moieties with distinct glycosylation profiles; however, the precise sugar sequence, monosaccharide composition, and glycosidic linkage positions remain to be confirmed through complementary NMR and hydrolysis studies. Although neither SEF-4 nor putative AgNP-SEF-4 displayed clinically relevant intrinsic antibacterial activity, the nanoformulation significantly enhanced the activity of aminoglycoside antibiotics. The most pronounced modulatory effects were observed against Klebsiella pneumoniae ATCC 1705 in combination with amikacin and against both standard and multidrug-resistant Escherichia coli strains when combined with gentamicin or amikacin. These findings highlight the potential of putative AgNP-SEF-4 as an antibiotic adjuvant capable of potentiating aminoglycoside efficacy and increasing bacterial susceptibility, including in multidrug-resistant strains. Full article

The contamination of aquatic environments by cadmium and other toxic heavy metals represents a major environmental concern requiring efficient and operationally sustainable remediation strategies. In this work, iron oxide materials were synthesized through a microwave-assisted hydrothermal method and evaluated for Cd(II) removal from aqueous systems. Different precursor compositions and organic additives were initially screened in order to identify the most suitable adsorbent formulation. The selected Fe-Tart material was characterized by FTIR, SEM-EDS, and XRD analyses, revealing hydroxylated and poorly crystalline iron oxide structures with heterogeneous surface organization. Batch adsorption experiments were performed under controlled conditions to investigate the influence of pH and equilibrium adsorption behavior, while adsorption data were analyzed using Langmuir and Freundlich isotherm models. Cd(II) uptake showed strong pH dependence, with adsorption progressively increasing from acidic to near-neutral conditions and reaching approximately 80% removal at pH 7–8. The Langmuir model provided the best fitting results (R² = 0.988), suggesting preferential occupation of energetically comparable surface sites with a maximum adsorption capacity of 6.51 mg g⁻¹. The adsorption behavior was interpreted within a pH-dependent surface complexation framework involving hydroxylated iron oxide surfaces. Although the adsorption capacity remained lower than that reported for some highly engineered adsorbents, the results indicate that microwave-assisted synthesis may provide a relatively simple and rapid route for preparing iron oxide-based materials potentially applicable to water remediation systems. Full article

This study examines the direct effects of R&D transfer and the sufficiency of financing for entrepreneurs on Technological Total Entrepreneurial Activity (TechTEA), as well as the mediating role of financing. The analysis employs a variance-based structural equation modeling approach (PLS-SEM) to explore causal relationships among the variables. Data are drawn from the Global Entrepreneurship Monitor (GEM), specifically the National Expert Survey (NES) and the Adult Population Survey (APS), covering 26 emerging economies. The findings reveal that R&D transfer has a significant positive effect on the sufficiency of financing available to entrepreneurs. However, R&D transfer shows a significant negative direct effect on TechTEA. In contrast, the sufficiency of financing has a positive impact on TechTEA, acting as an inconsistent mediator that neutralizes the negative direct friction of R&D and unlocks its positive indirect potential. The study demonstrates that Resource-Based View (RBV) principles operate in a fundamentally distinct manner within emerging economies. Raw technical knowledge (R&D transfer) and capital injections (sufficiency of financing) are not self-sufficient drivers of high-tech ventures in developing contexts. From a sustainability perspective, this dynamic suggests that institutional and ecosystem frictions can impede the Triple Bottom Line, as technological potential struggles to achieve the economic viability required for long-term societal impact. Consequently, achieving genuine ecosystem sustainability requires policymakers to shift away from isolated, supply-side resource injections. Future strategies must pivot toward comprehensive institutional governance, friction-reduction mechanisms, and cross-sector regulatory coordination to enable technological ventures to successfully scale and survive over time. Full article

The increasing generation of organic waste and the growing demand for sustainable solid fuels have intensified interest in hydrothermal carbonisation (HTC) as a pathway for biomass valorization within circular bioeconomy systems. HTC uses subcritical water to upgrade moist biomass into hydrochar with improved fuel properties and combustion behavior. This review correlates key HTC parameters, including temperature, residence time, pH, and the nature of feedstock, with the chemical evolution and thermal reactivity of different hydrochars. Data synthesis identifies a typical ‘kinetic optimization’ range between 180 and 220 °C for conventional lignocellulosic feedstocks. Within this thermal interval, activation energy (E_a) decreases from 180–260 kJ/mol for raw biomass to 70–180 kJ/mol for hydrochars, while the high heating value (HHV) reaches up to ~28 MJ/kg. The results further demonstrate that feedstock composition strongly influences combustion reactivity and kinetic behavior under similar HTC conditions. The integration of isoconversional methods with thermodynamic parameters (ΔH, ΔG, ΔS) confirms a transition toward more ordered and thermally stable carbon structures. Additionally, Criado’s master plots indicate a shift from diffusion-controlled to reaction-controlled combustion mechanisms with increasing HTC severity. These findings provide valuable insights into the optimizing of HTC conditions for balance energy densification and combustion reactivity, offering a comprehensive understanding to guide future hydrochar-based energy applications and scale-up studies. Full article

Background/Objectives: Despite overall excellent outcomes for Wilms tumour, regional variations in stage at diagnosis and care pathways remain a concern across Europe. We evaluated stage distribution, three-year survival, and treatment patterns in Italy, considering hospital care as a proxy for healthcare capacity and migration. Methods: Data were obtained from 26 population-based cancer registries (PBCRs), covering 148 patients (ages 0–14) diagnosed between 2013 and 2017, representing about 80% of the Italian population. Stage was classified according to the Toronto guidelines. Information on treatment and diagnosed/treating hospitals was collected. Stage at diagnosis was further refined using probabilistic linkage with the clinical registry 1.01 Model. Overall survival, defined as all-cause mortality, was estimated using the Kaplan–Meier method. Results: Most patients presented with localized disease (77%), 32% Stage I, while 19% were Stage IV. Three-year survival analysis showed significant differences between stages, ranging from 98% in patients with Stage I to 78% in the ones with Stage IV. No significant disparity across the Italian regions was observed in stage distribution or survival. Diagnoses and treatments were mostly (>90%) centralized in the same region for patients residing in the Centre or North of Italy. However, the cross-regional health migration from the South was of about 30% for diagnosis and larger for treatments. Conclusions: This study shows that standardized staging improves data comparability and highlights challenges in managing metastatic cases and regional care pathways. The results support the use of clinical and PBCR information to interpret survival patterns and guide improvements in paediatric oncology care. Full article

Objectives: Visual attention to emotional faces provides a useful framework for investigating orienting, visual exploration, and attentional engagement across development. The present study aimed to characterize the visuospatial organization of attention in neurotypical children and to examine how this pattern is modulated by social and emotional factors. Twenty children (aged 8–12 years) participated in a passive viewing paradigm of facial expressions while their eye movements were recorded using eye tracking (120 Hz). Methods: Oculomotor metrics based on areas of interest (eyes, mouth, nose, face, and non-social regions) were analyzed, including time to first fixation (TTFF), number of fixations (NF), and total fixation duration (TFD), as well as total saccade count as a global index of visual scanning. Results: Results indicated statistically significant AOI-dependent interactions involving emotional expression, observer sex, stimulus sex, and stimulus race/ethnicity, revealing region-specific modulation of visual attention. Consistently, prioritization of the eye region was observed, particularly for angry expressions, and was associated with greater fixation recurrence and duration, whereas happy and surprised expressions were associated with increased attentional allocation to the mouth. Differences related to observer sex and stimulus characteristics reflected region-specific modulations. In contrast, global saccadic dynamics remained relatively stable across experimental conditions and showed no significant effects of observer sex, stimulus sex, race/ethnicity, or emotional expression. Conclusions: Taken together, these findings suggest that visual attention to emotional faces in childhood follows a relatively stable spatial organization characterized by preferential processing of the eye region and region-specific modulation associated with emotional expression and stimulus characteristics. Full article

Food waste is one of the most pressing obstacles to sustainable development. Reducing food waste in schools and kindergartens constitutes an important component of sustainable waste management. To achieve this reduction, various interventions targeting food waste can deliver multiple benefits across environmental, social, and economic dimensions. Among these, behavioral “nudges” aim to steer consumer choices without restricting options. This study evaluated a novel nudging intervention in the canteens of two primary schools and one kindergarten, with the goal of reducing plate waste. The nudging intervention consisted of a simple, interactive installation designed to encourage children to reflect on their food consumption and portion choices. The installation was integrated into routine lunch service and it combined ball-based voting with visual prompts: the emptier the returned plate, the greater the voting weight for the pupil. Across all institutions the food waste level (soup and second dish combined) was significantly decreased during the nudging intervention: by 31% for primary school no. 84, 18% for school no. 1, and 33% for kindergarten no. 56, although part of this reduction was attributable to lower food production volumes. Plate waste for the second dish decreased in all the considered schools: by 10 g/meal (11%), 19 g/meal (22%), and 52 g/meal (51%), respectively. After the intervention a larger share of the second dish served was consumed than was left on the plates compared to the situation during the baseline monitoring. A shift from plate waste to unserved food, which was one of the goals of the study, could not be unambiguously confirmed. Overall, the new nudging installation appears effective. Substantial changes in food production complicate the possibility of determining the effects of the nudging intervention. Future research should maintain constant production levels across the baseline and intervention periods. In addition, pupils should be given maximum freedom to determine their portion sizes during the nudging intervention. The long-term effects of the nudging approach should also be evaluated. Full article