Search Results (6,647)

Pharmaceuticals and personal care products (PPCPs) are recognized as emerging contaminants of concern, even at ultra-trace concentrations. However, the current detection systems are prohibitively expensive and typically rely on labor-intensive, lab-based workflows that lack automation in sample pretreatment. In this study, we developed a robotic and on-flow solid-phase extraction (ROF-SPE) system, fully integrated with online liquid chromatography-tandem mass spectrometry (LC-MS/MS), for the on-site and real-time monitoring of 16 PPCPs in wastewater effluent. The system automates the entire pretreatment workflow—including sample collection, filtration, pH adjustment, solid-phase extraction, and injection—prior to seamless coupling with LC–MS/MS analysis. The optimized pretreatment parameters (pH 7 and 10, 12 mL wash volume, 9 mL elution volume) were selected for analytical efficiency and cost-effectiveness. Compared with conventional offline SPE methods (~370 min), the total analysis time was reduced to 80 min (78.4% reduction), and parallel automation significantly enhanced the throughput. The system was capable of quantifying target analytes at concentrations as low as 0.1 ng/L. Among the 16 PPCPs monitored at a municipal wastewater treatment plant in South Korea, only sulfamethazine and ranitidine were not detected. Compounds such as iopromide, caffeine, and paraxanthine were detected at high concentrations, and seasonal variation patterns were also observed This study demonstrates the feasibility of a fully automated and on-site SPE pretreatment system for ultra-trace environmental analysis and presents a practical solution for the real-time monitoring of contaminants in remote areas. Full article

Modern military aircraft integrate a large number of high-power-density electronic devices, which leads to a rapid increase in thermal load and poses significant challenges for heat dissipation. A promising thermal management approach is to intake ram air through a fuselage-mounted S-duct inlet and utilize it as a heat sink for the downstream heat exchanger. However, the S-duct’s geometry can induce significant flow separation and total pressure distortion, thereby limiting the mass flow rate. To address these challenges, this study investigates three flow-control strategies—vortex generators (VGs), Coanda injectors, and their combination—using high-fidelity three-dimensional numerical simulations validated against experimental data. The results indicate that VGs effectively suppress local separation and improve flow uniformity, although additional losses limit pressure recovery. The Coanda injector enhances boundary-layer momentum, substantially increasing mass flow throughput and pressure recovery. The combined VGs and Coanda injector approach achieves a lower distortion coefficient and provides a favorable balance between pressure recovery and flow uniformity. These findings demonstrate the potential of hybrid passive–active flow control in improving inlet aerodynamic quality and supporting integrated thermal management systems for future aircraft. Full article

In this study, a mathematical model was proposed for a continuous, multistage, industrial-scale alcoholic fermentation process, comprising four vats in series with volumes equal to 600 m³, with separation, acid treatment, and cell recycling from the fourth to the first vat. The system was operated daily under variable volumetric flow rates and substrate concentrations in the feed stream, i.e., F₀ = 93–127 m³/h and S₀ = 210–238 g/L. The mathematical model consisted of mass balance equations for cells, substrate, and product in the vats, the separator, and the acid treatment unit. An unsegregated and unstructured approach was used to describe the microbial population, with the kinetics of cell growth, substrate consumption, and product formation represented by equations generally adopted for alcoholic fermentation. The model parameters were estimated by nonlinear regression, providing typical values for alcoholic fermentation. Model predictions agreed well with both the experimental data used in the parameter estimation step and those used in the model validation step. Full article

Near-complete (>99%) dissolution of lithium and cobalt was achieved by the leaching of black mass from spent (end-of-life) lithium-ion batteries (LiBs) using 4 M H₂SO₄ or HCl at 60 °C. Raising the temperature to 90 °C did not increase the overall extraction of lithium or cobalt, but it increased the rate of extraction. At 60 °C, 2 M H₂SO₄ or 2 M HCl performed similarly to the 4 M H₂SO₄/HCl solution, although extractions were lower using 1 M H₂SO₄ or HCl (~95% and 98%, respectively). High extractions were also observed by leaching in low pulp density (15 g/L) at 60 °C with 2 M CH₂ClCOOH. Leaching was much slower with hydrogen peroxide reductant concentrations below 0.5 mol/L, with cobalt extractions of 90–95% after 3 h. Pulp densities of up to 250 g/L were tested when leaching with 4 M H₂SO₄ or HCl, with the stoichiometric limit estimated for each test based on the metal content of the black mass. Extractions were consistently high, above 95% for Li/Ni/Mn/Cu with a pulp density of 150 g/L, dropping sharply above this point because of insufficient remaining acid in the solution in the later stages of leaching. The final component of the test work used leaching parameters identified in the previous experiments as producing the largest extractions, and just sulphuric acid. A seven-stage semi-continuous sulphuric acid leach at 60 °C of black mass from LiBs that had undergone an oxidising roast (2h in a tube furnace at 500 °C under flowing air) to remove binder material resulted in high (93%) extraction of cobalt and near total (98–100%) extractions of lithium, nickel, manganese, and copper. Higher cobalt extraction (>98%) was expected, but a refractory spinel-type cobalt oxide, Co₃O₄, was generated during the oxidising roast as a result of inefficient aeration, which reduced the extraction efficiency. Full article

This study introduces a method for positioning film holes guided by conjugate isotherms. The aerodynamic performance exhibited by the turbine blade was evaluated, and the cooling effectiveness of various film hole configurations were systematically compared through combined numerical simulations and cascade wind tunnel experiments. Key influencing factors were investigated, and the underlying flow field structures and optimization mechanisms were elucidated. Numerical models reliably captured the aerodynamic and heat transfer characteristics, including pressure distribution and overall cooling effectiveness trends. Elevating the mass flow rate ratio was shown to enhance the overall cooling effectiveness across the blade surface. Modifications in film hole layout altered the cooling effectiveness along the blade region downstream of the holes and influenced cooling behavior in non-perforated areas near the endwall. While mid-blade cooling effectiveness showed minimal variation between Hole pattern #1 and #2, the latter exhibited superior overall cooling effectiveness at both the leading and trailing edges. Moreover, Hole pattern #2 diminished the temperature gradient between the suction and pressure sides, thereby augmenting blade structural integrity. Furthermore, Hole pattern #2 promoted a more even distribution of cooling effectiveness over the blade surface, leading to improved thermal protection. Therefore, strategic arrangement of film holes along conjugate isotherms serves as a vital approach for increasing gas turbine efficiency. Full article

To address low energy utilization efficiency and severe exergy destruction from direct discharge of high-temperature turbine exhaust, this study proposes a supercritical CO₂ Brayton cogeneration system with a series-connected hot water heat exchanger for stepwise waste heat recovery. Based on finite-time thermodynamics, a physical model that provides a more realistic framework by incorporating finite temperature difference heat transfer, irreversible compression, and expansion losses is established. Aiming to maximize exergy output rate under the constraint of fixed total thermal conductance, the decision variables, including working fluid mass flow rate, pressure ratio, and thermal conductance distribution ratio, are optimized. Optimization yields a 16.06% increase in exergy output rate compared with the baseline design. The optimal parameter combination is a mass flow rate of 79 kg/s and a pressure ratio of 5.64, with thermal conductance allocation increased for the regenerator and cooler, while decreased for the heater. The obtained results could provide theoretical guidance for enhancing energy efficiency and sustainability in S-CO₂ cogeneration systems, with potential applications in industrial waste heat recovery and power generation. Full article

Understanding and controlling thermal rectification is pivotal for designing phononic devices that guide heat flow in a preferential direction. This study investigates one-dimensional atomic chains with binary mass asymmetry and nonlinear interatomic potentials, focusing on how energy propagates under thermal and wave excitation. Two potential models—the β-FPU and Morse potentials—were employed to examine the role of nonlinearity and bond softness in energy transport. Simulations reveal strong directional energy transport governed by the interplay of mass distribution, nonlinearity, and excitation type. In FPU chains, pronounced rectification occurs: under “cold-heavy” conditions, energy in the left segment increases from ~1% to over 63%, while reverse (“hot-heavy”) cases show less than 4% net transfer. For wave-driven excitation, the rectification coefficient reaches ~0.58 at 100:1. In contrast, Morse-based systems exhibit weaker rectification (∆E < 1%) and structural instabilities at high asymmetry due to bond breaking. A comprehensive summary and heatmap visualization highlight how system parameters govern rectification efficiency. These findings provide mechanistic insights into nonreciprocal energy transport in nonlinear lattices and offer design principles for nanoscale thermal management strategies based on controlled asymmetry and potential engineering. Full article

Background: Endometriosis, believed by many to be rooted in immunology, is a chronic disease. Upregulation of programmed cell death protein 1 (PD-1) in immune cells may compromise their defensive function, a mechanism demonstrated in the context of cancer spread. This study aims to explore the potential involvement of PD-1 in the pathophysiology and progression of endometriosis. A total of 62 patients who underwent laparoscopic surgery were analyzed, with 47 diagnosed with endometriosis and 15 serving as controls. We collected peritoneal fluid and peripheral blood samples during surgery and examined them using flow cytometry. Using a panel of monoclonal antibodies, the samples were stained and the expression of PD-1 in immune cells was evaluated. Results: We observed a statistically significant rise in the percentage of the CD56⁺ CD16⁺ NK cell subset expressing PD-1 within the peritoneal fluid of endometriosis patients compared to the control group (p = 0.021). Similarly, we found that PD-1 expression on immune cells significantly differed based on factors such as body mass index and smoking habits. Moreover, peritoneal subsets of PD-1⁺ T and NK cells showed an increase in patients presenting symptomatic endometriosis and those with more widespread disease. Conclusions: Our evaluation of the inhibitory PD-1 receptor has strengthened the potential connection between immune escape mechanisms often seen in cancer cells and those in endometriotic cells. This concept could pave the way for future research in the field of immunomodulation and endometriosis. Full article

Asteroids are remnants of primordial material from the early stages of solar system formation, approximately 4.6 billion years ago, and they preserve invaluable records of the processes underlying planetary evolution. Investigating asteroids provides critical insights into the mechanisms of planetary development and the potential origins of life. To enable efficient sample acquisition under vacuum and microgravity conditions, this study introduces a two-stage gas-driven asteroid sampling strategy. This approach mitigates the challenges posed by low-gravity environments and irregular asteroid topography. A coupled computational fluid dynamics–discrete element method (CFD–DEM) framework was employed to simulate the gas–solid two-phase flow during the sampling process. First, a model of the first-stage gas-driven sampling device was developed to establish the relationship between the inlet angle of the gas nozzle and the sampling efficiency, leading to the optimization of the nozzle’s structural parameters. Subsequently, a model of the integrated two-stage gas-driven sampling and sample-delivery system was constructed, through which the influence of the second-stage nozzle inlet angle on the total collected sample mass was investigated, and its design parameters were further refined. Simulation outcomes were validated against experimental data, confirming the reliability of the CFD–DEM coupling approach for predicting gas–solid two-phase interactions. The results demonstrate the feasibility of collecting asteroid regolith with the proposed two-stage gas-driven sampling and delivery system, thereby providing a practical pathway for extraterrestrial material acquisition. Full article

This paper presents the development and evaluation of an autonomous solar dryer designed to enhance the drying efficiency of bee bread granules. In contrast to natural open-air drying, the proposed system utilizes solar energy in an oscillating operational mode to achieve a controlled and accelerated drying process. The dryer comprises a solar collector integrated into the base of the drying chamber, which facilitates convective heating of the drying agent (air). The system is further equipped with a photovoltaic panel to generate electricity for powering and controlling the operation of air extraction fans. The methodology combines numerical modeling with experimental studies, structured by an experimental design framework. The modeling component simulates variations in temperature (288–315 K) and relative humidity within a layer of bee bread granules subjected to a convective air flow. The numerical simulation enabled the determination of the following: the time required to achieve a stationary operating mode in the dryer chamber (20 min); and the rate of change in moisture content within the granule layer during conventional drying (18 h) and solar drying treatment (6 h). The experimental investigations focused on determining the effects of granule mass, air flow rate, and drying time on the moisture content and temperature of the granular layer of Bee Bread. A statistically grounded analysis, based on the design of experiments (DoE), demonstrated a reduction in moisture content from an initial 16.2–18.26% to a final 11.1–12.1% under optimized conditions. Linear regression models were developed to describe the dependencies for both natural and forced convection drying. A comparative evaluation using enthalpy–humidity (I-d) diagrams revealed a notable improvement in the drying efficiency of the proposed method compared to natural drying. This enhanced performance is attributed to the system’s intermittent operational mode and its ability to actively remove moist air. The results confirm the potential of the developed system for sustainable and energy-efficient drying of bee bread granules in remote areas with limited access to a conventional power grid. Full article

Mitochondrial proteins (mtPs) packaged into extracellular vesicles (EVs), particularly exosomes, have emerged as promising biomarkers and functional mediators in cancer biology. In this study, we investigated whether breast cancer (BC)-derived exosomes selectively incorporate mtPs, thereby providing insights into their diagnostic potential and role in tumor progression. Exosomes were isolated from conditioned media of multiple BC cell lines and non-tumorigenic breast epithelial cells using ultracentrifugation and were characterized by transmission electron microscopy and flow cytometry for canonical markers CD9 and CD81. Proteomic profiling by MALDI-TOF mass spectrometry revealed a distinct enrichment of mtPs in exosomes derived exclusively from tumor cells, while non-tumorigenic cells lacked such cargo. Identified proteins were predominantly associated with the oxidative phosphorylation system, with Complex I subunits most frequently detected, whereas Complex II components were entirely absent. These findings highlight a cancer-specific mechanism of exosomal mtP incorporation, potentially reflecting altered energy metabolism and stress responses in malignant cells. We conclude that mtPs in exosomes represent both functional contributors to tumor progression and promising candidates for liquid biopsy-based BC diagnostics. Full article

Background/Objectives: Ovarian adnexal masses present diagnostic challenges due to their heterogeneous etiologies. Accurately differentiating these conditions is critical for timely and effective clinical intervention. This study evaluated circulating molecules and serum-induced apoptosis as complementary tools to conventional diagnostic methods (CA125, HE4, and the ROMA index) for distinguishing benign masses from malignant masses. Methods: A cohort of 136 participants (9 healthy controls, 87 women with benign ovarian adnexal masses and 40 with malignant ovarian adnexal masses) was analyzed. The induction of apoptosis in Jurkat cells by patient serum was assessed using flow cytometry. Serum concentrations of sFas/CD95, HE4, CA125, and additional molecules were measured by ELISA and LEGENDplex™. Clinical, ultrasonographic, and histopathological data were correlated with tumor malignancy. To improve diagnostic performance beyond individual biomarkers, we developed two multiparametric classifiers that integrate the dominant parameters identified through group divergence analysis and ROC evaluation across multiple clinical comparisons. Results: Malignant tumors were associated with older age (51.45 ± 8.35 years, p = 0.0002), postmenopausal status (61.1%, p = 0.0013), and larger tumor size (>10 cm). Ultrasonographic features of complexity were observed exclusively in malignant masses. Functional assays revealed reduced apoptosis in Jurkat cells exposed to malignant sera, suggesting tumor-mediated immune evasion. Although higher sFas levels were observed in tumors, no significant differences were identified between the groups. Among the circulating biomarkers, CA125, HE4, MRP8/14, OPN, and SAA levels were significantly higher in malignant tumors than in benign tumors and controls. Conclusions: The evaluation of CA125, HE4, MRP8/14, and apoptosis (Classifier 1) and, more prominently, the measurement of additional molecules: OPN, SAA, IL-6, IL-8, and IGFBP-4 (Classifier 2), systematically outperformed the ROMA. Both achieved superior specificity and balanced accuracy (Youden’s J index) across all clinical comparisons by capturing the biological diversity of malignancies. Full article

People with HIV (PWH) are at an increased risk for AIDS-associated non-Hodgkin lymphoma (AIDS-NHL); however, the immune signatures underlying this risk are not well understood. In this study, we utilized mass cytometry by time-of-flight (CyTOF) to analyze T-cells and monocytes in the PBMCs of treatment-naïve PWH, including those 3 to 36 months before an AIDS-NHL diagnosis (HIV-positive pre-NHL), as well as people without HIV (PWoH). Mass cytometry is an advanced single-cell analysis platform that combines flow cytometry principles with mass spectrometry. Unlike conventional flow cytometry, this technology employs antibodies conjugated to unique metal isotopes instead of fluorescent markers, enabling simultaneous measurement of over 40 distinct cellular markers per individual cell without spectral overlap limitations. Participants were enrolled at the Los Angeles site of the MACS/WIHS Combined Cohort Study (MWCCS). Unsupervised clustering and Uniform Manifold Approximation and Projection (UMAP) analysis identified CD3⁺ T-cell and CD14⁺ monocyte metaclusters, and Spearman’s rank correlation assessed their relationships with B-cell subsets exhibiting aberrant phenotypes. We observed elevated levels of CD8⁺CD20⁺ T-cells, CD8⁺CD14⁺ T-cells, and M2-like CD14⁺CD163⁺ monocytes in HIV-positive pre-NHL individuals compared to HIV-negative controls. Positive correlations were found between CD19⁺ AICDA⁺ cMYC⁺ B-cells and M1-like CD14⁺cMYC⁺ monocytes (metacluster, MC02), and between metaclusters of CD8⁺PD-1⁺CD27⁺CXCR4⁻ T-cells (MC05) and CD4⁺FoxP3⁺PD-1⁺CD27⁺CD28⁺CXCR4⁻ ICOS⁺ T-cells (MC08). In addition, a different CD19⁺ B-cell metacluster (FoxP3⁺AICDA⁺cMYC⁺) was positively associated with a metacluster of CD8⁺PD-1⁺CD27⁺CD28⁺CXCR4⁺ T-cells (MC03). Moreover, the metacluster of CD8⁺PD-1⁺CD27⁺CXCR4⁻ T-cells (MC05) negatively correlated with M2-like CD14⁺CD163⁺ monocytes (MC06), while CD8⁺CD14⁺ T-cells positively correlated with AICDA⁺ Bregs and IL-10⁺ B-regs in HIV-positive pre-NHL individuals. Unsupervised analysis revealed increased frequencies of CD8⁺CD20⁺ T-cells in HIV-positive individuals compared to HIV-negative controls. These immune alterations provide valuable insights into potential biomarkers for early detection, monitoring, and therapeutic strategies for AIDS-NHL. Full article

Objectives: Despite achieving a high rate of revascularization in epicardial coronary arteries with primary percutaneous coronary intervention (pPCI), suboptimal coronary reperfusion is encountered in more than half of patients. This condition, termed the ‘no-reflow phenomenon’ (NRP), has been associated with ventricular arrhythmias, left ventricular dysfunction, impaired ventricular remodeling, myocardial reinfarction, and increased mortality. The neutrophil percentage/albumin ratio (NPAR) has been associated with the severity and prognosis of cardiovascular patients. The aim of this study is to investigate the relationship between NRP and NPAR in patients undergoing pPCI with a diagnosis of ST-elevation myocardial infarction (STEMI). Methods: A total of 758 patients diagnosed with STEMI and undergoing pPCI were included in this study. A total of 105 patients were detected to have NFP during pPCI (13.8%). Slow flow, such as thrombolysis in myocardial infarction (TIMI) 0, 1, or 2, observed in the distal part of the coronary artery after pPCI, was operationally defined as NRP. Reflow was defined as TIMI 3. NPAR was obtained by dividing the neutrophil percentage by albumin. Results: Statistically, there was a significant difference between the groups in terms of mean age, body mass index (BMI), and left ventricular ejection fraction (LVEF), which were higher in the NRP group [54 (45–62) vs. 60 (53–67), 26.5 (23.6–30.8) vs. 28.4 (26–31), and 39.2 ± 6.9 vs. 31.8 ± 5.1; p < 0.001, for all]. When laboratory parameters were examined between the two groups, white blood cell (WBC) count, neutrophil count, neutrophil percentage, C-reactive protein (CRP), neutrophil/lymphocyte ratio (NLR), NPAR and CRP/albumin ratio (CAR) levels were found to be statistically significantly higher in the patient group with NRP (p < 0.05). Multivariate analysis identified NPAR as an independent predictor of NRP (5.482, 3.254–9.234, p < 0.001). ROC analysis demonstrated that the best cutoff value of 18.45 for NPAR was to predict NRP with 80% sensitivity and 75% specificity (area under ROC curve = 0.826 (95% CI: 0.770–0.881), p < 0.001). Conclusions: We found that NPAR levels at admission were independently associated with the development of NRP pPCI in patients with STEMI. Full article

The present work investigated numerically turbulent airflows over a hybrid Darrieus/Savonius vertical axis wind turbine. Firstly, the isolated turbines were validated in comparison to previous studies from the literature. Later, new recommendations were obtained for the simulation of a hybrid turbine subject to turbulent airflow. The numerical simulations consisted of the solution of time-averaged equations of mass and momentum in x and y directions using the finite volume method, available in the commercial code Ansys Fluent (version 2022 R1). For closure of turbulence, the k − ω SST (Shear Stress Transport) model was employed. For lower magnitudes of tip speed ratio (TSR), the hybrid turbine improved the power coefficient (C_P) compared to the Darrieus turbine (e.g., by 70% at TSR = 0.75), thereby demonstrating the self-starting capability of the hybrid configuration. Unexpectedly, at the optimal TSR = 1.5, the hybrid turbine performed about 6.5% better than the Darrieus turbine, indicating that the balance between the additional power generated by the Savonius rotor and losses caused by flow disturbances in the hybrid configuration was positive. As a novelty, results highlighted the role of each rotor (Darrieus and Savonius) for the performance of the hybrid turbine by comparing it with isolated Darrieus and Savonius turbines under the same conditions. Full article