Search Results (179)

Single-use plastic residues persist in agricultural and peri-urban soils of the Ecuadorian Andes. Regionally sourced starch-based films are a plausible local replacement for short-lifetime petroleum plastics, yet field-relevant degradation data for tropical high-altitude soils remain scarce. This study evaluated the soil biodegradability of bioplastic films produced from Colocasia esculenta (malanga blanca) and Manihot esculenta (yuca) across three contrasting soils from Chimborazo, Ecuador (ESPOCH, San Andrés and Río Chimborazo; 2825–3249 m a.s.l.) as a function of their physicochemical properties and bibliographically inferred microbial context. The films were prepared by citric acid starch extraction, glycerol plasticization and carboxymethylcellulose reinforcement; the gravimetric weight loss was tracked on days 0, 11, 18, 27, 40 and 47 on n = 20–21 film replicates per soil × feedstock combination, with the soils characterized by their pH, electrical conductivity and organic matter. After 47 days, the malanga films reached 42.3 ± 13.6%, 22.9 ± 10.7% and 54.1 ± 19.3% mean (±standard deviation, SD) weight loss in the ESPOCH, San Andrés and Río Chimborazo soils, respectively; the yuca films reached 24.4 ± 6.5%, 21.1 ± 6.8% and 49.4 ± 18.7%. The between-soil differences were statistically significant at 47 days according to the analysis of variance (ANOVA) (malanga: F = 22.17, p < 0.001; yuca: F = 34.08, p < 0.001; Tukey’s Honestly Significant Difference (HSD)), with the results corroborated by the Kruskal–Wallis method (H = 29.16 and 37.05; both p < 0.001), given the partial departure from normality identified by the Shapiro–Wilk test. The ordering of degradation departed from the bulk organic matter ranking, indicating that microbial community composition, rather than organic matter quantity alone, was the proximal driver. These findings extend the scarce evidence base on cassava/taro film degradation under high-Andean conditions. Full article

A comprehensive multiregional characterization of the spectral response of cassava leaves across different ontogenetic stages was performed. For this, ultraviolet (UV), visible (VIS) and shortwave near-infrared (UV-VIS-NIR; 200–900 nm) regions were used to identify spectral signatures and indices for their potential use as biomarkers of leaf development and physiological status of plants under induced senescence conditions. Manihot esculenta Crantz (HMC-1 variety) was used as a model. Spectral signatures were obtained from leaves at two phenological stages (4 and 6 months after planting) using UV-VIS-NIR spectroscopy by the diffuse reflectance technique. Classical and experimental spectral indices were evaluated, and their discriminatory power through different ontogenies was assessed using ANOVA/Kruskal–Wallis and post hoc tests. Senescence effects were further examined by postharvest monitoring (1–20 days), with temporal, ontogenetic, and interaction effects validated using linear mixed models (LMMs), while multivariate structure and spectral convergence were explored via principal component analysis and hierarchical clustering (PCA-HCA). Functionally Enhanced Derivative Spectroscopy (FEDS), comparative analysis, and spectral correlation mapping allowed signal’s selective enhancement and the identification of phenolic compounds, photosynthetic pigments, and structural molecular components. Results showed high ontogenetic stability of UV-associated phenolic signals (~210–220 nm), whereas the VIS region (420–600 nm) clearly differentiated young leaves. The NIR region was stable across ontogeny but highly sensitive to temporal degradation, reflecting changes in water status and internal structure. UV-VIS-NIR indices effectively differentiated young leaves and changes by stress. It is concluded that multiregional characterization of the spectral response supported by FEDS allows the extraction of robust indices with strong potential as biomarkers of leaf maturation and senescence in cassava. Full article

Background: Xyloglucan endotransglucosylase/hydrolase (XTH) acts as a key cell wall-modifying enzyme and contributes to plant stress resilience. This study aimed to identify the MeXTH gene family in cassava and characterize its potential functions in abiotic stress adaptation. Method: A full set of bioinformatic analyses was performed, including phylogeny, gene structure, conserved motifs, chromosomal localization, synteny, promoter cis-elements and subcellular localization. Expression patterns were examined by quantitative real-time PCR (qRT-PCR). Results: Forty-two MeXTH genes were identified and distributed on 14 chromosomes, encoding proteins with conserved Glyco_hydro_16 (Glycoside hydrolase family 16) and XET_C (Xyloglucan endotransglycosylase C-terminal domain) domains. Genes were clustered into four subfamilies with similar structures. Synteny was closer between cassava and dicots than monocots. Twenty-four stress-, hormone- and light-related cis-elements were detected. Ten MeXTH genes showed obvious differential expression under stress, and most proteins were located in the cell wall. Conclusions: The MeXTH gene family is structurally conserved and can serve as a readout of abiotic stress in cassava. These results provide a theoretical basis for molecular breeding aimed at enhancing stress resistance in cassava. Full article

Diet plays a critical role in shaping the composition of gut microbiota in insects. Samia ricini, an economically important Lepidoptera insect, is a polyphagous herbivore that offers a useful model for studying dietary effects on the animal gut microbiome. Here, we fed S. ricini larvae with different food plants, Ricinus communis, Ailanthus altissima, and Manihot esculenta leaves to investigate how host plant species influence growth performance, digestive enzyme activities, and the gut microbial community. Our results showed that the Ricinus group exhibited better growth performance. Regarding digestive enzymes, the midgut lipase activity was significantly higher in the Ricinus group than in the Ailanthus group, while no significant differences were observed in α-amylase, cellulase, or trypsin activities among the three groups. Compared to the Manihot group, the Ricinus group showed increased bacterial richness, while the Ailanthus group showed increased bacterial diversity. β-diversity analysis further revealed distinct microbial community structures among all three dietary groups. Specifically, Acinetobacter, Mammaliicoccus, Roseateles, Methylobacterium, Agrobacterium, Faecalibacterium, and Segatella were the dominant bacterial genera. Functional prediction revealed that gut microbes enriched in the Ricinus group were associated with terpenoid/polyketide metabolism, xenobiotics biodegradation, and glycan biosynthesis, whereas those involved in carbohydrate metabolism and biosynthesis of other secondary metabolites were higher in the Manihot group. Spearman correlation analysis indicated that Methylobacterium, Methylorubrum, and Agrobacterium were significantly positively correlated with larval weight, while Staphylococcus and Cyanothece_PCC-7424 exhibited negative correlations. Collectively, these findings suggest a potential association between different plant-derived diets, gut microbiota composition, and host growth performance, highlighting the pivotal role of diet in shaping insect gut microbial communities. Full article

The drying kinetics of three varieties of cassava were evaluated in a tray dryer, using a completely randomized design with a three-factor factorial arrangement: temperature (50, 60, and 70 °C), air velocity (1, 2, and 3 m/s), and variety (“Blanca Mona”, “Ica Negrita”, “Venezolana”), with three replicates per treatment. The results obtained were used to construct drying curves, which showed that this process occurred in the decreasing period. The drying curves were adjusted to mathematical models, and the Page model was the best fit to the experimental data with R²_adj values closer to 1 and RSS values less than 0.0086. The effective diffusivities (D_eff) in cassava flours were represented by the Arrhenius equation with values ranging from 5.24 × 10⁻¹⁰ to 1.58 × 10⁻⁹ m²/s. The activation energy (E_a) recorded values between 20.34 and 28.32 kJ/mol. The flours from the three cassava varieties were obtained under the best drying conditions (70 °C and 3 m/s). The physicochemical characterization of fresh roots and flours from three cassava varieties revealed significant genotype-dependent differences in their proximal composition. Blanca Mona exhibited the highest ash content and the lowest total carbohydrates among fresh roots, while Ica Negrita stood out for its superior crude fiber content in flour. Venezolana flour stood out for its higher protein content (3.86 ± 0.04 g/100 g) and significant fiber content (1.39 ± 0.39 g/100 g), making it the flour with the best nutritional profile and greatest potential for food applications. Therefore, tray drying is recommended as one of the suitable methods for cassava flour production. Full article

This study addresses the imperative need to extend the shelf life of the cape gooseberry (Physalis peruviana L.), a highly perishable yet nutritionally valuable fruit, through the development and optimization of active edible coatings (ECs). The synergy between cassava starch (Manihot esculenta Crantz) and lemon verbena essential oil (Aloysia citrodora), both bioactive components, was investigated for the formulation of protective coatings. A 2² factorial design explored the impact of cassava starch concentrations (8% and 10% w/v) and lemon verbena essential oil (LVEO) (1% and 3% v/v) on the sensory acceptability of coated cape gooseberries. Through binomial logistic regression analysis, it was determined that the formulation with 10% cassava starch and 3% LVEO (T4) exhibited significantly superior sensory acceptability, optimizing the perception of color, odor, flavor, texture, and overall appearance. This optimized formulation (T4) demonstrated a significant improvement in extending the shelf life of cape gooseberries up to 27 days at 10 °C, which is comparable to or exceeds values reported in previous studies on starch–based coatings in similar fruits (e.g., 15–21 days depending on formulation and storage conditions). This performance also exceeded the storage periods observed at 6 °C (6 days) and 8 °C (20 days). Physicochemical analyses revealed remarkable stability of pH and titratable acidity, as well as effective control of moisture loss and the maturity index, even at higher temperatures. Crucially, T4 exhibited superior antimicrobial activity, with a significant reduction in molds, yeasts, and total aerobes, particularly at 10 °C, suggesting an optimal synergistic interaction between the coating and the LVEO under slightly warmer storage conditions. These findings contribute to the advancement of sustainable preservation strategies of cape gooseberries, offering a sustainable solution that reconciles efficient shelf-life extension with consumer acceptability and optimizes storage conditions, with significant implications for reducing food waste and enhancing the global marketability of this fruit. Full article

Cassava (Manihot esculenta Crantz) is a nitrogen-efficient crop that can achieve high biomass production on poor soils. However, the mechanisms underlying the response of cassava to nitrogen-deficiency signals and the regulation of nitrogen use efficiency remain unclear. Here, we found that MeCEPD (MeGRXC1) was specifically induced by CEP6 peptides and low nitrate, and showed higher expression in leaves and stems. Overexpression of MeCEPD enhanced cassava’s tolerance to nitrate deficiency by upregulating the expression of MeNRT2.1, MeNRT2.4, and MeRBCS1A, which was manifested as increased root biomass, greater lateral root number, and darker leaf coloration. In contrast, the MeCEPD-edited lines exhibited a statistically significant reduction in root length, plant height, and biomass compared to the wild-type. Additionally, nitrate deficiency accelerated leaf senescence. Furthermore, yeast two-hybrid (Y2H) assay revealed that MeCEPD interacts with the photosynthesis-related MeRBCS1A and lateral root development-related MeLHW, which may regulate nitrogen use efficiency. Unlike its Arabidopsis thaliana homologs AtCEPD1/2 and AtCEPDL2, which interact with AtTGA1/4, MeCEPD does not interact with MeTGA1 yet still upregulates MeNRT2.1 expression. These findings contribute to our understanding of the complex regulatory mechanisms underlying cassava’s adaptation to low-nitrogen conditions and could provide new information for genetic improvement in nitrogen use efficiency in cassava. Full article

As a typical C3-C4 intermediate plant, cassava (Manihot esculenta Crantz) exhibits high photosynthetic efficiency and low photorespiration. NADP-malic enzyme (NADP-ME) is a key enzyme in the C4 photosynthetic pathway that provides elevated ${\mathrm{CO}}_2$ concentrations for Rubisco. However, research on NADP-ME in C3-C4 intermediate species remains limited. In this study, we identified four NADP-ME genes in the cassava genome, with segmental duplication serving as the primary driving force for gene evolution. Cis-acting element analysis indicated potential roles of MeNADP-ME genes in environmental adaptation, stress responses, and growth regulation. Expression profiling using bulk RNA sequencing and single-cell RNA sequencing revealed distinct expression patterns in different tissues and cell subsets. Comparative analysis with Arabidopsis (Arabidopsis thaliana) and maize (Zea mays) NADP-ME families demonstrated that MeNADP-ME3 exhibits bundle sheath cell-specific expression analogous to ZmchlC4NADP-ME in maize. Notably, photosynthetic genes and plasmodesmata (PD)-related genes exhibited high co-expression within mesophyll subcluster 13 and bundle sheath cells, providing molecular evidence for a limited C4 photosynthetic pathway in cassava. Protein–protein interaction predictions implicated MeNADP-ME3 in photosynthetic carbon metabolism and photorespiration regulation. Furthermore, qRT-PCR revealed significant responsiveness of MeNADP-ME3 to various abiotic stresses, and confocal imaging confirmed its chloroplast localization. Functional validation demonstrated that Arabidopsis overexpressing MeNADP-ME3 exhibited 30–120% enhanced antioxidant enzyme activities (SOD, POD, CAT) and 20–32% reduced oxidative damage markers (MDA, ${\mathrm{H}}_2{\mathrm{O}}_2$) under drought and salt stresses. These findings reveal the evolutionary trajectory of NADP-ME genes in C3-C4 intermediate species and provide genetic resources for developing stress-tolerant cassava cultivars. Full article

The rising demand for sustainable and functional ingredients necessitates the development of novel replacers for traditional food components, such as eggs, which are critical for structure and aeration in baked goods. This study investigated hydrocolloids derived from cassava (Manihot esculenta) as a partial egg substitute in sponge cakes, evaluating their effect on rheological, physicochemical, nutritional, and sensory properties. The resulting cake batter exhibited characteristic non-Newtonian, pseudoplastic, and viscoelastic fluid behavior. A microstructural analysis confirmed that the stabilized, higher-viscosity doughs successfully facilitated the formation of larger, more stable air bubbles, effectively mimicking the structural role of the egg. Physicochemical assessments demonstrated a high product equivalence; the fat content showed no significant difference (p < 0.05) compared to the control, while pH and carbohydrate levels decreased. Crucially, the optimized formula, CK-S50-H2.5 (50% egg and 2.5% hydrocolloids substitutions), exhibited a minimal color difference (ΔE) consistent with the control, preserving product appearance. Sensory evaluation confirmed that hydrocolloid substitution did not compromise consumer acceptance. Panelists preferred cakes utilizing lower egg substitution levels for their enhanced flavor and texture. These findings establish that cassava hydrocolloids serve as an effective and functional partial egg replacer, yielding a high-quality and well-accepted product and offering a valuable, sustainable solution for the food industry. Full article

Livestock feed shortage is a serious global problem, worsened by climate-change-induced droughts that continue to disrupt its production, consequently threatening food and nutrition security. Drought poses a significant threat to conventionally farmed feed crops, such as maize and soybeans, reducing their availability and negatively impacting the livestock industry. These crops cannot withstand intense drought, creating a need for alternative feed sources with good nutritional value, positive health benefits and livestock performance, as well as cost-reduction potential for farmers. Research continues to explore drought-tolerant crops such as sorghum (Sorghum bicolor), millet (Pennisetum glaucum and Eleusine coracana), cassava (Manihot esculenta), false banana (Ensete ventricosum), and cactus pear (Opuntia ficus-indica) for use as traditional feed substitutes or in hybrid feedstock production to enhance food security, support farmers, and conserve the environment. Unlike the conventional feed crops, these underutilized crops are tolerant under arid conditions, use less water, and possess higher nutritional value, making them important for climate change adaptation and sustainable agricultural systems. Despite the growing recognition of drought-tolerant crops in livestock feed systems, a comprehensive review discussing the advancements and potential of these types of crops as livestock feed is lacking in the literature. Therefore, this review discusses the critical role of selected key drought-tolerant crops as alternative livestock feed, covering the drivers for their use, utilization and processing studies, quality determinants, associated challenges, and sustainable innovation strategies to inform policy making. Full article

Continuous monocropping of cassava (Manihot esculenta Crantz) often leads to soil degradation and yield decline, commonly referred to as continuous cropping obstacles (CCOs), which are closely linked to changes in soil physicochemical properties and microbial communities. Biochar has been widely used as a soil amendment to improve soil quality and microbial activity and is considered a potential strategy for alleviating CCOs. Understanding the effects of biochar on soil bacteria and metabolites under field conditions is essential, as it provides insights into its practical effectiveness in reducing CCOs and improving soil health in cassava cultivation systems. In this study, a field experiment was conducted in a continuous cassava system to investigate the effects of a single biochar application rate on soil bacterial diversity, community composition, and metabolite profiles in both rhizosphere and bulk soils. High-throughput 16S rRNA gene sequencing and UHPLC–MS/MS-based non-targeted metabolomics were employed to analyze soil bacterial and metabolic patterns. Biochar was associated with increased α-diversity in rhizosphere soil and distinct shifts in β-diversity. Biochar increased the relative abundance of Chloroflexi and Actinobacteriota in the bulk soil, while Cyanobacteria and Nitrospirota were more abundant in the rhizosphere. Network analysis revealed the compartment-specific differences after biochar application, with higher network complexity in the rhizosphere and lower complexity in the bulk soil relative to the control. Metabolomic profiling identified 402 metabolites in positive ion mode and 357 in negative ion mode. In the rhizosphere, biochar-treated soil exhibited higher relative abundances of alkaloids (e.g., trigonelline, berberine, vincristine) and flavonoids (e.g., catechin, naringin, rutin, and taxifolin), which are commonly linked to plant stress responses. In the bulk soil, biochar application resulted in lower levels of several anthropogenic organic compounds (e.g., monobutyl phthalate, terephthalic acid, and p–toluenesulfonic acid). These findings provide preliminary field evidence that biochar application can lead to compartment-specific changes in soil bacterial communities and metabolite profiles. Such changes are closely related to soil quality and nutrient cycling, pointing to a possible role of biochar in mitigating soil degradation under continuous cassava cultivation. Full article

This research focused on the systematic engineering of processing parameters to obtain novel hydrocolloids from cassava (Manihot esculenta), specifically investigating how extraction pH controls their functional and physicochemical properties. Hydrocolloids were obtained across a range of pH conditions, followed by rigorous analysis of their chemical composition, flow behavior, viscoelasticity, and technological capacity, including water and oil holding capacity (WHC and OHC). The study established that hydrocolloids yield can be decoupled from extreme pH constraints, as high yields were successfully attained in both acidic and alkaline environments, thereby identifying a critical and flexible processing window for scalable production. Compositionally, the extracts confirmed their potential as functional additives due to a high carbohydrate content and minimal fat. Crucially, the extracted hydrocolloids exhibited strong structural performance, displaying high water and oil retention capacity—metrics essential for emulsion stability and shelf life—while consistently confirming desirable shear-thinning behavior across all effective extraction conditions. In conclusion, these results demonstrate that hydrocolloids derived from cassava are versatile stabilizers whose robust structural performance is maintained across varying processing pH levels, positioning them as promising, cost-effective alternatives for developing resilient, stable food matrices. Full article

The genus Xanthomonas comprises devastating plant pathogens responsible for significant yield losses in globally critical crops such as rice (Oryza sativa L.), citrus (Citrus L. spp.), cassava (Manihot esculenta Crantz), and tomato (Solanum lycopersicum L.). This review synthesizes current knowledge on the molecular mechanisms driving Xanthomonas pathogenicity, including the type III secretion system (T3SS) that translocates effector proteins, transcription activator-like effectors (TALEs) that reprogram host transcription, and extracellular polysaccharides (EPS) that promote biofilm formation and immune evasion, which collectively enable host colonization, immune suppression, and disease progression. Rapid adaptation through genomic plasticity and horizontal gene transfer (HGT) exacerbates challenges in disease management by facilitating evasion of host defenses and environmental stressors. Economically, Xanthomonas spp. inflict billions in annual losses through crop damage, trade restrictions, and eradication efforts, disproportionately affecting resource-limited regions. Emerging antibiotic resistance and climate-driven shifts in pathogen distribution further threaten food security. Sustainable strategies, such as CRISPR-based genome editing to disrupt susceptibility genes, biocontrol agents (e.g., Bacillus and Pseudomonas spp.), and nanotechnology-driven antimicrobials offer promising alternatives to conventional copper-based and chemical controls. This review underscores the urgent need for integrated, climate-resilient management approaches to mitigate the ecological and socioeconomic impacts of Xanthomonas diseases, bridging genomic insights with innovative control measures, to address escalating threats posed by these pathogens in a changing global climate. Full article

Background: Cassava (Manihot esculenta Crantz) ranks as the sixth largest food crop worldwide and serves as an important cash and energy crop. Heavy-metal-associated isoprenylated plant proteins (HIPPs) are metallochaperones involved in metal homeostasis and stress adaptation in vascular plants. However, research on the identification and function of HIPPs in cassava has been poorly explored. Methods: This study conducted a pan-genome-wide investigation to identify and characterize MeHIPPs in 31 cassava accessions. Subsequent analyses examined their physicochemical properties, subcellular localization, phylogeny, Ka/Ks, chromosomal localization, synteny, gene structure, and cis-acting elements. Additionally, the expression profiles of MeHIPPs in different tissues and cell subsets and under different stress conditions were analyzed using transcriptome data and quantitative real-time polymerase chain reaction (qRT-PCR). Results: A total of 59 MeHIPP pan-genes were identified, including five core genes, 22 softcore genes, 17 dispensable genes, and 15 private genes, which were unevenly distributed on chromosomes. Based on phylogenetic analysis, these genes were classified into five major subgroups. Evolutionary analyses indicated that segmental duplication predominated in family expansion and that most members may be subjected to purifying selection. Cis-element analysis highlighted the importance of MeHIPPs in plant adaptation to environmental stress. The expression profiles suggested widespread involvement of MeHIPP genes in response to Xanthomonas phaseoli pv. manihotis (Xpm) infection and drought stress. Different MeHIPP genes exhibited varying transcript levels in different tissues and cell subsets. qRT-PCR analysis revealed that the selected MeHIPP genes had distinct expression patterns under Cd stress. Conclusions: This study provides valuable insights into the functional characteristics of MeHIPP genes and their evolutionary relationships, laying a theoretical foundation for further functional research on stress resistance. Full article

Cassava (Manihot esculenta) is a staple crop in sub-Saharan Africa threatened by several viral diseases. Here, we describe the genome sequence of a novel bipartite cheravirus (family Secoviridae) infecting cassava in the Democratic Republic of Congo and Tanzania. We designate the new virus “cassava Congo cheravirus”. Each RNA segment encodes a single polyprotein (P1 and P2 for RNA1 and RNA2, respectively), embedded with various putative cleavage sites (six and three in P1 and P2, respectively), consistent with members of the genus Cheravirus. We note two new features in the P1: (i) the presence of two domains, X1 and X2, upstream of the putative helicase region, which we also predict in other cheraviruses and (ii) the presence of a Maf/HAM1-like inosine triphosphatase (ITPase) domain, a rare motif among viruses only previously detected in three potyviruses and a torradovirus, all of which infect plants from the Euphorbia family. Phylogenetic analyses placed the virus firmly within the genus Cheravirus, with amino acid identities in the Pro-Pol and coat protein regions well below existing ICTV species thresholds, supporting its classification as a virus belonging to a new species in the Cheravirus genus. Spatially distinct isolates from Bas-Congo, South-Kivu, and Tanzania form three genetic clusters, with evidence of recombination in both RNA segments. These results expand the known diversity of cassava viruses and suggest possible adaptation to the cassava host via ITPase acquisition. Full article