Search Results (255)

In marine engineering applications, substituting conventional crushed stone coarse aggregates with coral aggregates offers dual advantages: reduced terrestrial quarrying operations and minimized construction material transportation costs. However, the inherent characteristics of coral aggregates—low bulk density, high porosity, and elevated water absorption capacity—adversely influence concrete workability and mechanical performance. To address these limitations, this investigation employed microbial-induced carbonate precipitation (MICP) for aggregate modification. The experimental design systematically evaluated the impacts of substrate concentration (1 mol/L) and mineralization period (14 days) on three critical parameters, mass gain percentage, water absorption reduction, and apparent density enhancement, across distinct particle size fractions (4.75–9.5 mm, 9.5–20 mm) and density classifications. Subsequent application trials assessed the performance of MICP-treated aggregates in marine concrete formulations. Results indicated that under a substrate concentration of 1 mol/L and mineralization period of 14 days, lightweight coral aggregates and coral aggregates within the 4.75–9.5 mm size fraction exhibited favorable modification effects. Specifically, their mass gain rates reached 11.75% and 11.22%, respectively, while their water absorption rates decreased by 32.22% and 34.75%, respectively. Apparent density increased from initial values of 1764 kg/m³ and 1930 kg/m³ to 2050 kg/m³ and 2207 kg/m³. Concrete mixtures incorporating modified aggregates exhibited enhanced workability and strength improvement at all curing ages. The 28-day compressive strengths reached 62.1 MPa (11.69% increment), 46.2 MPa (6.94% increment), and 60.1 MPa (14.91% increment) for the 4.75–9.5 mm, 9.5–20 mm, and continuous grading groups, respectively, compared to untreated counterparts. Full article

In order to study how indigenous microorganisms can enhance the strength properties of granite residual soil in the Hanzhong area, two Bacillus species that produce urease were isolated from the local soil. The two Bacillus species are Bacillus subtilis and Bacillus tequilensis, and they were used for the solidification and improvement of the granite residual soil. Unconfined compressive strength tests, scanning electron microscope (SEM) and X-ray diffraction (XRD) analyses were systematically used to analyze the influence and mechanism of different cementation solution concentrations on the improvement effect. It has been found that with the growth of cementing fluid concentration, the unconfined compressive strength of improved soil specimens shows an increasing tendency, reaching its highest value when the cementing solution concentration is 2.0 mol/L. Among different bacterial species, curing results vary; Bacillus tequilensis demonstrates better performance across various cementing solution concentrations. The examination of failure strain in improved soil samples indicates that brittleness has been successfully alleviated, with optimal outcomes obtained at a cementing solution concentration of 1.0 mol/L. SEM and XRD analyses show that calcium carbonate precipitates (CaCO₃) are formed in soil samples treated by both strains. These precipitates effectively bond soil particles, verifying improvement effects on a microscopic level. The present study proposes an environmentally friendly and economical method for enhancing engineering applications of granite residual soil in Hanzhong area, which holds significant importance for projects such as artificial slope filling, subgrade filling, and foundation pit backfilling. Full article

The dramatic increase in urban construction waste poses severe environmental challenges. Utilizing waste concrete to produce recycled aggregates (RA) for manufacturing recycled concrete (RC) represents an effective strategy for resource utilization. However, inherent defects in RA, such as high porosity, microcracks, and adherent old mortar layers, lead to significant performance degradation of the resulting RC, limiting its widespread application. Traditional methods for enhancing RA often suffer from limitations, including high energy consumption, increased costs, or the introduction of new pollutants. MICP offers an innovative approach for enhancing RC performance. This technique employs the metabolic activity of specific microorganisms to induce the formation of a three-dimensionally interwoven calcium carbonate gel network within the pores and on the surface of RA. This gel network can improve the inherent defects of RA, thereby enhancing the performance of RC. Compared to conventional techniques, this approach demonstrates significant environmental benefits and enhances concrete compressive strength by 5–30%. Furthermore, embedding mineralizing microbial spores within the pores of RA enables the production of self-healing RC. This review systematically explores recent research advances in microbial mineral gel network for improving RC performance. It begins by delineating the fundamental mechanisms underlying microbial mineralization, detailing the key biochemical reactions driving the formation of calcium carbonate (CaCO₃) gel, and introducing the common types of microorganisms involved. Subsequently, it critically discusses the key environmental factors influencing the effectiveness of MICP treatment on RA and strategies for their optimization. The analysis focuses on the enhancement of critical mechanical properties of RC achieved through MICP treatment, elucidating the underlying strengthening mechanisms at the microscale. Furthermore, the review synthesizes findings on the self-healing efficiency of MICP-based RC, including such metrics as crack width healing ratio, permeability recovery, and restoration of mechanical properties. Key factors influencing self-healing effectiveness are also discussed. Finally, building upon the current research landscape, the review provides perspectives on future research directions for advancing microbial mineralization gel techniques to enhance RC performance, offering a theoretical reference for translating this technology into practical engineering applications. Full article

Soil improvement is one of the fundamental practices in civil engineering, with a long-standing history. In today’s context, the rapidly increasing demand for construction driven by urbanization has further emphasized the necessity and significance of soil stabilization techniques. This study aims to determine the optimum parameters for improving sandy soils by incorporating sodium alginate (SA) as a biopolymer additive into the microbial calcium carbonate precipitation (MICP) process. Sand types S1, S2, and S3, each with distinct particle size distributions, were selected, and the specimens were prepared at medium relative density. Three distinct approaches, MICP, SA, and MICP + SA, were tested for comparison. Additionally, two different improvement methods, injection and mixing, were applied to investigate their effects on the geotechnical properties of the soils. In this context, hydraulic conductivity, unconfined compressive strength (UCS), and calcite content tests, as well as scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) analyses, were performed to assess the changes in soil behavior. SA contributed positively to the overall efficiency of the MICP process. The study highlights SA-assisted MICP as an alternative that enhances the microstructural integrity of treated soils and responds to the environmental limitations of conventional methods through sustainable innovation. Full article

Microbially-induced calcite precipitation (MICP) has emerged as a promising bio-geotechnical technique for sustainable soil improvement, yet accurate prediction of treatment effectiveness remains challenging due to complex multi-factor interactions. This study develops an ensemble learning framework (LARO-EnML) for predicting the unconfined compressive strength (UCS) of MICP-treated sand. A comprehensive database containing 402 experimental datasets was utilised in the study, consisting of unconfined compression test results from bio-cemented sands with eight key input parameters considered. The performance evaluation demonstrates that LARO-EnML achieves superior predictive accuracy, with RMSE of 0.5449, MAE of 0.2853, R² of 0.9570, and OI of 0.9597 on the test data, significantly outperforming other models. Model interpretability analysis reveals that calcite content serves as the most influential factor, with a strong positive correlation to strength enhancement, while urease activity exhibits complex, staged influence characteristics. This research contributes to advancing the practical implementation of MICP technology in geotechnical engineering by offering both accurate predictive capability and enhanced process understanding through interpretable ML approaches. Full article

Microbially induced carbonate precipitation (MICP) offers an eco-friendly approach to stabilize porous materials. This study evaluates its feasibility for protecting agricultural drainage ditch slopes through laboratory tests. Liquid experiments assessed calcium carbonate (CaCO₃) precipitation rates under varying bacteria–cementation solution ratios (BCR), cementation solution concentrations (1–2 mol/L), and urease inhibitor (NBPT) contents (0–0.3%). Soil experiments further analyzed the effects of solidified layer thickness (4 cm vs. 8 cm) and curing cycles on soil stabilization. The results showed that CaCO₃ precipitation peaked at a BCR of 4:5 and declined when NBPT exceeded 0.1%. Optimal parameters (0.1% NBPT, 1 mol/L cementation solution, BCR 4:5) were applied to soil tests, revealing that multi-cycle treatments enhanced soil water retention and CaCO₃ content (up to 7.6%) and reduced disintegration rates (by 70%) and permeability (by 83%). A 4 cm solidified layer achieved higher Ca²⁺ utilization, while an 8 cm layer matched or exceeded 4 cm performance with shorter curing. Calcite crystals dominated CaCO₃ formation. Crucially, reagent dosage should approximate four times the target layer’s requirement to ensure efficacy. These findings demonstrate that MICP, when optimized, effectively stabilizes ditch slopes using minimal reagents, providing a sustainable strategy for agricultural soil conservation. Full article

Microbially induced calcium carbonate precipitation (MICP) has emerged as a research focus in concrete crack remediation due to its environmental compatibility and efficient mineralization capacity. The hypersaline conditions of seawater (average 35 g/L NaCl) and alkaline environments (pH 12) within concrete cracks pose significant challenges to the survival of mineralization-capable microorganisms. To enhance microbial tolerance under these extreme conditions, this study employed a laboratory adaptive evolution strategy to successfully develop a Sporosarcina pasteurii strain demonstrating tolerance to 35 g/L NaCl and pH 12. Comparative analysis of growth characteristics (OD₆₀₀), pH variation, urease activity, and specific urease activity revealed that the evolved strain maintained growth kinetics under harsh conditions comparable to the parental strain under normal conditions. Subsequent evaluations demonstrated the evolved strain’s superior salt–alkali tolerance through enhanced enzymatic activity, precipitation yield, particle size distribution, crystal morphology, and microstructure characterization under various saline–alkaline conditions. Whole-genome sequencing identified five non-synonymous mutated genes associated with ribosomal stability, transmembrane transport, and osmoprotectant synthesis. Transcriptomic profiling revealed 1082 deferentially expressed genes (543 upregulated, 539 downregulated), predominantly involved in ribosomal biogenesis, porphyrin metabolism, oxidative phosphorylation, tricarboxylic acid (TCA) cycle, and amino acid metabolism. In concrete remediation experiments, the evolved strain achieved superior performance with 89.3% compressive strength recovery and 48% reduction in water absorption rate. This study elucidates the molecular mechanisms underlying S. pasteurii’s salt–alkali tolerance and validates its potential application in the remediation of marine engineering. Full article

Erosion poses significant threats to infrastructures and ecosystems, exacerbated by climate change-driven sea-level rise and intensified wave actions. Microbially induced calcium carbonate precipitation (MICP) has emerged as a promising, sustainable, and eco-friendly solution for erosion mitigation. This review synthesizes recent advancements in optimizing biomineralization efficiency, multi-scale erosion control, and field-scale MICP implementations in marine dynamic conditions. Key findings include the following: (1) Kinetic analysis of Ca²⁺ conversion confirmed complete ion utilization within 24 h under optimized PA concentration (3%), resulting in a compressive strength of 2.76 MPa after five treatment cycles in ISO-standard sand. (2) Field validations in Ahoskie and Sanya demonstrated the efficacy of MICP in coastal erosion control through tailored delivery systems and environmental adaptations. Sanya’s studies highlighted seawater-compatible MICP solutions, achieving maximum 1743 kPa penetration resistance in the atmospheric zone and layered “M-shaped” CaCO₃ precipitation in tidal regions. (3) Experimental studies revealed that MICP treatments (2–4 cycles) reduced maximum scour depth by 84–100% under unidirectional currents (0.3 m/s) with the maximum surface CaCO₃ content reaching 3.8%. (4) Numerical simulations revealed MICP enhanced seabed stability by increasing vertical effective stress and reducing pore pressure. Comparative analysis demonstrates that while the destabilization depth of untreated seabed exhibits a linear correlation with wave height increments, MICP-treated seabed formations maintain exceptional stability through cohesion-enhancing properties, even when subjected to progressively intensified wave forces. This review supports the use of biomineralization as a sustainable alternative for shoreline protection, seabed stabilization, and offshore foundation integrity. Full article

Expansive soils exhibit pronounced behaviors of swelling, shrinkage, and over-consolidation, leading to significant engineering challenges. To address these issues, this study investigated the influence of different cementation solution concentrations on the swelling and mechanical properties of microbially induced calcium carbonate precipitation (MICP)-treated expansive soils. Additionally, a series of wet–dry cycling experiments were conducted to analyze the performance degradation of the MICP-treated soils under cyclic environmental conditions. The results showed that as the cementation solution concentration increased, the free swell ratio and unconfined swell ratio of the soil samples decreased by 39.29% and 71%, respectively. The unconfined compressive strength, cohesion, and internal friction angle of the MICP-treated soils also exhibited significant improvements, with increases of 391.67%, 33.76%, and 91.67%, respectively. A cementation solution concentration of 1 M was found to be the optimal for achieving substantial enhancements in the soil properties. However, under continuous wet–dry cycling, the mechanical properties of the MICP-treated soils experienced degradation, with the unconfined compressive strength decreasing by up to 62.24%. These findings highlight the potential of MICP as an effective and eco-friendly technique for stabilizing expansive soils. Full article

Cohesive soil in the reservoir area is vulnerable to natural disasters because of its poor erosion resistance and low strength. Therefore, it needs to be reinforced. Microbially induced calcium carbonate precipitation (MICP) is a sustaibable soil reinforcement technique with low energy consumption and no pollution. Different combinations of Bacillus subtilis bacterial solution (BS) concentrations and cementing solution (CS) concentrations were set to perform MICP solidification treatment. The characterization of cohesive soil before MICP was carried out by means of Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), and Laser Particle Size Analyzer (LPSA). The results showed that the unreinforced soil showed an amorphous state with low strength and the particle size distribution was dominated by powder particles. However, with the addition of BS concentrations and CS concentrations, SEM results showed that spherical and rhombohedral minerals filled the pores of the cohesive soil, which increased the content of precipitations and enhanced the cementitious characteristics. When the concentrations of CS or BS were fixed, CaCO₃ content, deviatoric stress, shear strength, cohesive force, and internal friction angle all showed a trend of first increasing and then decreasing with the increase in CS or BS concentration. The optimal combination of CS and BS concentration was 1.5 mol/L and OD₆₀₀ = 1.8. Thermochemical analyses showed an improved thermal stability of the reinforcing cohesive soil, with the lowest mass loss (32%) and the highest pyrolysis temperature (812 °C) of the samples at the optimal combination of BS and CS concentration. This study is expected to improve the understanding of the MICP reinforcement process and contribute to the optimal design of future biologically mediated soil amendments, promoting bioremediation. Full article

The carbon emissions from the cement industry account for approximately 8% of global carbon emissions, which exerts significant pressure on the environment. In this paper, the microbial-induced calcium carbonate precipitation (MICP) technology was introduced into the carbonization modification research of recycled hardened cement powder (RHCP), and the carbon sequestration performance of RHCP under different pressures was studied. The physicochemical properties of the carbonated products were characterized by microscopic testing methods, and the carbon sequestration mechanism under different pressures was obtained. Subsequently, carbonated RHCP (C-RHCP) was tested as a partial cement substitute for solidified sludge to evaluate its mechanical and durability properties. The results show that when the pressures were 0.3 and 0.5 MPa, the carbon sequestration capacity of RHCP was relatively good, reaching 59.14 and 59.82 g/kg, respectively. Since the carbon sequestration amounts under the two pressures were similar, and considering the energy consumption, in this study, a reaction pressure of 0.3 MPa was selected to prepare C-RHCP. Compared with pure cement, the 28-day unconfined compressive strength (UCS) of the sludge cured with 30% C-RHCP increased by 12.08%. The water stability coefficient of the solidified sludge in the C-RHCP group was greater than 1 after soaking for 7, 14, and 21 days, while the water stability coefficient of the cement group decreased to 0.92 at 14 days. After 20 freeze–thaw cycles, the mass losses of the cement group, the RHCP group, and the C-RHCP group were 31.43%, 38.99%, and 33.09%, respectively. This research not only provides an environmentally friendly strategy for the resource utilization of RHCP but also pioneers a new synergistic model that combines microbial mineralization with the modification of industrial solid waste. It demonstrated significant scientific value and engineering application prospects in reducing carbon emissions in the cement industry and promoted sustainable geotechnical engineering practices based on the “waste–waste” principle. Full article

Microbially induced calcite precipitation (MICP) has attracted attention as an environmentally friendly soil stabilization method, with Sporosarcina pasteurii being a key ureolytic bacterium in this process. However, its behavior in oxygen-limited environments remains poorly understood, limiting the predictability of MICP outcomes in natural soils. This study investigated the population dynamics of Sporosarcina in compacted soil reactors operated under aerobic and anaerobic conditions, including saturated environments. Quantitative PCR and 16S rRNA gene sequencing revealed that Sporosarcina thrived and became dominant under aerobic, unsaturated conditions, but failed to maintain a high abundance under anaerobic or saturated conditions. These findings indicate that gas-phase oxygen—not merely its presence in the overlying atmosphere—is essential for effective Sporosarcina-driven MICP. The results highlight a critical environmental constraint that limits the application of biostimulation strategies relying on indigenous Sporosarcina in oxygen-poor soils. This study provides the first in situ evidence linking oxygen availability and microbial dominance in MICP systems, with implications for optimizing microbial soil stabilization in real-world conditions. Full article

Aeolian sand is susceptible to wind and water erosion, which seriously restricts the ecological restoration and sustainable development in desert areas. Traditional solidification methods have characteristics of high cost, easy pollution, and unstable solidification. Enzyme-induced calcium carbonate precipitation (EICP) is an emerging method that has advantages in terms of cost-effectiveness, environmental friendliness, and durability, and, especially when coupled with fiber reinforcement (FR), it can significantly prevent brittle fracture. In this paper, ultraviolet (UV) erosion and freeze–thaw (FT) erosion tests were conducted to investigate the anti-erosion capacity of aeolian sand solidified by EICP and basalt fiber reinforcement (BFR) or wool fiber reinforcement (WFR). According to the analysis of the variation laws of sample appearance, quality losses, and unconfined compressive strength (UCS) during the UV and FT erosion process, the erosion mechanism was revealed, and the UCS models considering the damage effects were established. The research results indicated that the UCS of aeolian sand solidified by MICP and FR was significantly improved under UV and FT erosion. The strength loss rates of aeolian sand solidified by EICP, EICP–BFR, and EICP–WFR reached 45.4%, 46.6%, and 51.6%, respectively, under 90 h UV erosion. When the FT cycles reached 8, the strength loss rate of aeolian sand solidified by EICP, EICP–BFR, and EICP–WFR attained 41.0%, 49.2%, and 55.8%, respectively. The determination coefficients of the UCS models were all greater than 0.876, indicating that the experimental results were in good agreement with the predicted results, verifying the reliability of the established models. The research results can offer reference values for windproof and sand fixation in desert areas. Full article

Traditional materials synthesis often involves energy-intensive processes with significant waste generation and limited control over material properties. This review examines synthetic biology as a sustainable alternative for designing advanced materials with enhanced precision and versatility. It explores microbial biomineralization, detailing how microorganisms influence the formation of mineral deposits and participate in key biogeochemical cycles. It highlights recent research advancements in using a wide variety of microorganisms for the synthesis of inorganic materials such as metal and metal oxide nanoparticles, quantum dots, magnetic nanoparticles, and thin films. The review also discusses the production and properties of various biopolymers. Important factors that can influence the size, morphology, and uniformity of these biomaterials are covered in detail. Emphasis is placed on advancements utilizing synthetic biology tools, such as protein engineering and genome editing, and recent research for creating smart and responsive materials. Considering the present limitations of synthetic biology, challenges related to scale-up, yield, and uniformity are discussed, and suggestions for future research are detailed. Full article

The formation and spatial uniformity of calcium carbonate (CaCO₃) are critical for evaluating the effectiveness of microbial-induced calcium carbonate precipitation (MICP) in geotechnical applications. In recent years, the single-phase injection method has emerged as a promising alternative to traditional two-phase processes by addressing the issue of uneven CaCO₃ distribution. This study proposes a dual inhibition strategy that delays the mineralization reaction by synergistically lowering pH and temperature, thereby promoting uniform precipitation and enhanced compressive strength in cemented sand columns. A series of experiments, including bacterial growth, aqueous reaction, sand column reinforcement, and microstructural characterization, were conducted. Results show that the minimum pH required for flocculation increases from ~4.5 at 40 °C to ~6.0 at 10 °C. Under dual inhibition, the lag period effectively improved the spatial uniformity of CaCO₃ and enabled complete calcium utilization within 24 h. After four treatment cycles, the CaCO₃ content at 10 °C increased by 53%, and the unconfined compressive strength reached 2.5 MPa, a 50% improvement over the 40 °C condition. XRD analysis confirmed that calcite was the dominant phase (85–90%), accompanied by minor vaterite. These findings demonstrate the adaptability and efficiency of the dual inhibition method across temperature ranges, providing a cost-effective solution for broader engineering applications. Full article