Search Results (1,328)

Magnetic resonance imaging (MRI) super-resolution (SR) enables high-resolution reconstruction from low-resolution acquisitions, reducing scan time and easing hardware demands. However, most deep learning-based SR models are large and computationally heavy, limiting deployment in clinical workstations, real-time pipelines, and resource-restricted platforms such as low-field and portable MRI. We introduce CHARMS, a lightweight convolutional–Transformer hybrid with attention regularization optimized for MRI SR. CHARMS employs a Reverse Residual Attention Fusion backbone for hierarchical local feature extraction, Pixel–Channel and Enhanced Spatial Attention for fine-grained feature calibration, and a Multi-Depthwise Dilated Transformer Attention block for efficient long-range dependency modeling. Novel attention regularization suppresses redundant activations, stabilizes training, and enhances generalization across contrasts and field strengths. Across IXI, Human Connectome Project Young Adult, and paired 3T/7T datasets, CHARMS (~1.9M parameters; ~30 GFLOPs for 256 × 256) surpasses leading lightweight and hybrid baselines (EDSR, PAN, W2AMSN-S, and FMEN) by 0.1–0.6 dB PSNR and up to 1% SSIM at ×2/×4 upscaling, while reducing inference time ~40%. Cross-field fine-tuning yields 7T-like reconstructions from 3T inputs with ~6 dB PSNR and 0.12 SSIM gains over native 3T. With near-real-time performance (~11 ms/slice, ~1.6–1.9 s per 3D volume on RTX 4090), CHARMS offers a compelling fidelity–efficiency balance for clinical workflows, accelerated protocols, and portable MRI. Full article

Cannabinoids can bind to several cannabinoid receptors and modulate cellular signaling and gene expression relevant to inflammation and lipid homeostasis. Likewise, several vitamin E analogs can modulate inflammatory signaling and foam cell formation in macrophages by antioxidant and non-antioxidant mechanisms. We analyzed the regulatory effects on the expression of genes involved in cellular lipid homeostasis (e.g., CD36/FAT cluster of differentiation/fatty acid transporter and scavenger receptor SR-B1) and inflammation (e.g., inflammatory cytokines, TNFα, IL1β) by cannabinoids (cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC)) in human THP-1 macrophages with/without co-treatment with natural alpha-tocopherol (RRR-αT), natural RRR-αTA (αTAn), and synthetic racemic all-rac-αTA (αTAr). In general, αTAr inhibited both lipid accumulation and the inflammatory response (TNFα, IL6, IL1β) more efficiently compared to αTAn. Our results suggest that induction of CD36/FAT mRNA expression after treatment with THC can be prevented, albeit incompletely, by αTA (either αTAn or αTAr) or CBD. A similar response pattern was observed with genes involved in lipid efflux (ABCA1, less with SR-B1), suggesting an imbalance between uptake, metabolism, and efflux of lipids/αTA, increasing macrophage foam cell formation. THC increased reactive oxygen species (ROS), and co-treatment with αTAn or αTAr only partially prevented this. To study the mechanisms by which inflammatory and lipid-related genes are modulated, HEK293 cells overexpressing cannabinoid receptors (CB1 or TRPV-1) were transfected with luciferase reporter plasmids containing the human CD36 promoter or response elements for transcription factors involved in its regulation (e.g., LXR and NFκB). In cells overexpressing CB1, we observed activation of NFκB by THC that was inhibited by αTAr. Full article

We present a rapid, energy-efficient, and ecofriendly route for the synthesis of alkaline earth titanate perovskites—CaTiO₃, SrTiO₃, and BaTiO₃—using an affordable, commercially available CO₂ laser engraver, commonly found in makerspaces and small-scale workshops. The method involves direct laser irradiation of compacted pellets composed of low-cost, abundant, and non-toxic precursors: TiO₂ and alkaline earth carbonates (CaCO₃, SrCO₃, BaCO₃). CaTiO₃ and BaTiO₃ were synthesized with phase purities exceeding 97%, eliminating the need for conventional high-temperature furnaces or prolonged thermal treatments. X-ray diffraction (XRD) coupled with Rietveld refinement confirmed the formation of orthorhombic CaTiO₃ (Pbnm), cubic SrTiO₃ (Pm3⁻m), and tetragonal BaTiO₃ (P4mm). Raman spectroscopy independently corroborated the perovskite structures, revealing vibrational fingerprints consistent with the expected crystal symmetries and Ti–O bonding environments. All samples contained only small amounts of unreacted anatase TiO₂, while BaTiO₃ exhibited a partially amorphous fraction, attributed to the sluggish crystallization kinetics of the Ba–Ti system and the rapid quenching inherent to laser processing. Transmission electron microscopy (TEM) revealed nanoparticles with average sizes of 50–150 nm, indicative of localized melting followed by ultrafast solidification. This solvent-free, low-energy, and highly accessible approach, enabled by widely available desktop laser systems, demonstrates exceptional simplicity, scalability, and sustainability. It offers a compelling alternative to conventional ceramic processing, with broad potential for the fabrication of functional oxides in applications ranging from electronics to photocatalysis. Full article

Experimental and numerical investigations of the alternating current (AC) susceptibility, $\mathsf{\chi }\left(H\right) ~ \mathrm{d}M/\mathrm{d}H,$ examined multigrain La_0.66Sr_0.34MnO₃ (LSMO) thin films (thickness d = 250 nm) grown by radio-frequency (RF) magnetron sputtering on lattice-mismatched yttria-stabilized zirconia YSZ(001) substrates. The films exhibit a columnar structure comprising two types of grains, with (001)- and (011)-oriented planes of a pseudocubic lattice aligned parallel to the film surface. Field- and angle-dependent AC susceptibility measurements at 78 K reveal characteristic peak- and tip-like anomalies, attributed to contributions from grains with three distinct directions of easy magnetization axes within the film plane. Numerical modeling based on the transverse susceptibility theory for single-domain ferromagnetic grains, incorporating first- and second-order anisotropy constants, corroborates the experimental findings and elucidates the role of different grain types in magnetization switching and AC susceptibility response. This study provides a quantitative determination of the three in-plane easy magnetization axes in LSMO/YSZ(001) films and clarifies their influence on the magnetization dynamics of multigrain thin films. The demonstrated control over multigrain LSMO/YSZ(001) thin films with distinct in-plane easy magnetization axes and well-characterized AC susceptibility suggests potential applications in magnetic memory, spintronic devices, and precision magnetic sensing. Full article

Background: The apicomplexan parasite Toxoplasma gondii causes serious diseases in animals and humans. The in vitro efficacy of the antimicrobial peptide mixture tyrothricin, composed of tyrocidines and gramicidins, against T. gondii tachyzoites was investigated. Methods: Effects against T. gondii were determined by monitoring inhibition of tachyzoite proliferation and electron microscopy, host cell and splenocyte toxicity was measured by Alamar blue assay, and early embryo toxicity was assessed using zebrafish embryos. Differential affinity chromatography coupled to mass spectrometry and proteomics (DAC-MS-proteomics) was employed to identify potential molecular targets in T. gondii cell-free extracts. Results: Tyrothricin inhibited T. gondii proliferation at IC₅₀s < 100 nM, with tyrocidine A being the active and gramicidin A the inactive component. Tyrothricin also impaired fibroblast, T cell and zebrafish embryo viability at 1 µM. Electron microscopy carried out after 6 h of treatment revealed cytoplasmic vacuolization and structural alterations in the parasite mitochondrion, but these changes appeared only transiently, and tachyzoites recovered after 96 h. Tyrothricin also induced a reduction in the mitochondrial membrane potential. DAC-MS-proteomics identified 521 proteins binding only to tyrocidine A. No specific binding to gramicidin A was noted, and four proteins were common to both peptides. Among the proteins binding specifically to tyrocidine A were several SRS surface antigens and secretory proteins, mitochondrial inner and outer membrane proteins associated with the electron transfer chain and porin, and several calcium-binding proteins putatively involved in signaling. Discussion: These results suggest that tyrocidine A potentially affected multiple pathways important for parasite survival and development. Full article

This study developed high-performance anti-flashover silicone coatings using sol–gel-synthesized CaCO₃/SiO₂ hierarchical fillers optimized via L₁₆(4⁵) orthogonal design. The optimal filler (Sample 5) was prepared under 70 vol% ethanol, with nTEOS:nCaCO₃ = 1:1 and 0.2 mol/L NH₃·H₂O, at 45 °C, for 18 h, featuring covalent Si-O-Ca bonding, a dual-scale microstructure (2–4 μm CaCO₃ cores + 20–40 nm SiO₂ nodules), a 14.44 m²/g specific surface area, and bimodal porosity (8–80 nm). Composite C7 (30 wt% filler, 3 wt% KH-570, 1:2 resin-to-filler ratio) achieved superhydrophobicity (a 153° contact angle via Cassie-Baxter stabilization), ultrahigh electrical insulation (3.20 × 10¹⁴ Ω·cm volume resistivity, 1.60 × 10¹³ Ω surface resistivity), and robust mechanical properties (Shore 3H hardness, 5B adhesion). Standardized IEC 60507:2020 tests showed that C7’s flashover voltages (14.8 kV for KMnO₄, 14.3 kV for NaCl/KMnO₄, 13 kV for NaCl) exceeded that of neat silicone resin (NSR) and conventional CaCO₃-filled composite (SR-CC) by >135%. Additionally, C7 retained superhydrophobicity after 500 h UV aging and maintained a 124° contact angle after 12 months of outdoor exposure. The superior performance stems from synergistic hierarchical topology, tortuous discharge paths, and interfacial passivation. This work establishes a microstructure-driven design paradigm for grid protection materials in harsh environments. Full article

Background: Alternative pre-mRNA splicing is a combinatorial process involving serine/arginine-rich (SR) and heterogeneous nuclear ribonucleoprotein (hnRNP) splicing factors. These proteins can silence or enhance splicing based on their expression levels and binding positions. Objectives: To better understand the combinatorial and interdependent regulation between SR and hnRNP splicing factors during alternative splicing. Methods: Computational analyses were performed using cell knockdown and binding datasets from available databases. Results: Analyses of differential splicing data for 9 SR proteins and 21 hnRNP knockdowns revealed statistically significant interdependent regulation among several RNA-binding protein (RBP) combinations, albeit at different levels. Neither SR proteins nor hnRNPs showed strong preferences for collaborating with specific RBP classes in mediating exon inclusion. While SRSF3, hnRNPK, hnRNPC, and hnRNPL stand out as major influencers of alternative splicing, they do so predominantly independent of other RBPs. Minor influencers of alternative splicing, such as hnRNPDL and hnRNPR, predominantly regulate exon inclusion in concert with other RBPs, indicating that exon inclusion can be mediated by both single and multiple RBPs. Interestingly, the higher the number of RBPs that regulate the inclusion of an exon, the more variable exon inclusion preferences become. Interdependently regulated exons are more modular and can be characterized by weaker splice sites compared to their independently regulated counterparts. A comparison of RBP interdependence between HeLa and other cell lines provides a framework that explains cell-type-specific alternative splicing. Conclusions: Our study highlights the importance of the interdependent regulation of alternative exons and identifies characteristics of interdependently regulated exons that differ from independently regulated exons. Full article

Highly accurate quantitative detection of heavy metals is crucial for preventing environmental pollution and safeguarding public health. To address the demand for sensitive and specific detection of Cu²⁺ ions, we have developed carbon dots using a simple hydrothermal process. The synthesized carbon dots are highly stable in aqueous media, environmentally friendly, and exhibit strong blue photoluminescence at 440 nm when excited at 352 nm, with a quantum yield of 5.73%. Additionally, the size distribution of the carbon dots ranges from 2.0 to 20 nm, and they feature excitation-dependent emission. They retain consistent optical properties across a wide pH range and under high ionic strength. The photoluminescent probes are selectively quenched by Cu²⁺ ions, with no interference observed from other metal cations such as Ag⁺, Ca²⁺, Cr³⁺, Fe²⁺, Fe³⁺, Hg²⁺, K⁺, Mg²⁺, Sn²⁺, Pb²⁺, Sr²⁺, and Zn²⁺. The emission of carbon dots exhibits a strong linear correlation with Cu²⁺ concentration in the range of 0–14 μM via a static quenching mechanism, with a detection limit (LOD) of 4.77 μM in water. The proposed carbon dot sensor is low cost and has been successfully tested for detecting Cu²⁺ ions in general water samples collected from rivers in Taiwan. Full article

Background/Objectives: Athletes face both daily and sport-related stressors while being expected to perform at an optimal level. Effective recovery, particularly adequate sleep, plays a key role in psychophysiological restoration and performance, whereas sleep deprivation may impair functioning and increase perceived stress. This study examined the associations between coping strategies, sleep quality, athletic experience, competitive level, and perceived stress during the pre-competition period among female volleyball players. Methods: Ninety-one athletes (aged 18–35, M = 23.03, SD = 4.37) from three Polish professional leagues—Tauron (n = 31), First League (n = 30), and Second League (n = 30)—completed an online battery including the Stress Coping Strategies in Sport Questionnaire (SR3S), the Perceived Stress Scale (PSS-4), the Pittsburgh Sleep Quality Index (PSQI), and a demographic survey. Results: Based on PSQI scores, approximately 60% of the athletes were classified as poor sleepers. No significant differences in sleep quality or perceived stress were found across leagues. However, athletes competing in higher leagues reported more frequent use of mental coping strategies. Athletic experience, sleep quality, and the coping strategy of seeking social support were significantly associated with perceived stress. Players with less experience, poorer sleep, and a greater tendency to seek social support reported higher stress levels. The positive association between support-seeking and stress likely reflects reactive coping among more stressed athletes rather than a maladaptive effect of social support. Conclusions: These findings underscore the importance of promoting adaptive coping and sleep hygiene in competitive sport, particularly among less experienced female athletes during the pre-competition period. Full article

Background/Objectives: Super-resolution deep-learning reconstruction (SR-DLR) is an advanced image reconstruction technique, but its effect on dynamic myocardial computed tomography perfusion (CTP) imaging has not been evaluated. This study aimed to examine the impact of SR-DLR on image quality and perfusion parameters in dynamic myocardial CTP. Methods: Thirty-five patients who underwent dynamic myocardial CTP for coronary artery disease assessment were retrospectively analyzed. Two CTP datasets were reconstructed using hybrid iterative reconstruction (HIR) and SR-DLR. Image quality was compared qualitatively and quantitatively, including image noise, signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and edge rise slope (ERS). Equivalence of CT-derived myocardial blood flow (CT-MBF) between two reconstructions was tested using a previously reported 15% equivalence margin. Intra-patient variability of CT-MBF was evaluated using the robust coefficient of variation (rCV). Results: In the qualitative assessment, SR-DLR had significantly higher scores in contrast (4.0 vs. 2.0) and sharpness (4.5 vs. 2.5) compared with HIR (p < 0.001), while contrast scores were similar. In the quantitative assessment, SR-DLR demonstrated significantly lower image noise (19.4 vs. 29.4 HU), and improved SNR (6.1 vs. 4.1), CNR (13.7 vs. 10.9), and ERS (171.0 vs. 135.1 HU/mm) (all p < 0.001). Mean global CT-MBF was comparable (3.15 ± 0.91 mL/g/min for HIR vs. 3.18 ± 0.97 mL/g/min for SR-DLR) and equivalence was confirmed (p = 0.022). SR-DLR significantly reduced rCV compared with HIR (36.0% vs. 41.0%, p < 0.001). Conclusions: SR-DLR enhances image quality in dynamic myocardial CTP while maintaining mean global CT-MBF and reducing intra-patient variability. Full article

Shiqian County, located within a key geothermal fluids belt in Guizhou Province, China, has abundant underground hot water resources. Therefore, elucidating the hydrogeochemical characteristics and formation mechanisms of thermal mineral water in this area is essential for evaluating and sustainably utilizing regional geothermal fluids. This study focuses on the Shiqian Hot Spring Group and employs integrated analytical techniques, including rock geochemistry, hydrogeochemistry, isotope hydrology, digital elevation model (DEM) data analysis, remote sensing interpretation, geological surveys, mineral saturation index calculations, and PHREEQC-based inverse hydrogeochemical modeling, to elucidate its hydrogeochemical characteristics and formation mechanisms. The results show that strontium concentrations range from 0.06 to 7.17 mg/L (average 1.65 mg/L) and metasilicic acid concentrations range from 19.46 to 65.51 mg/L (average 33.64 mg/L). Most samples meet the national standards for natural mineral water and are classified as Sr-metasilicic acid type. Isotope analysis indicates that the geothermal water is recharged by meteoric precipitation at elevations between 911 m and 1833 m, mainly from carbonate outcrops and fracture zones on the southwestern slope of Fanjingshan, and discharges south of Shiqian County. The dominant hydrochemical types are HCO₃·SO₄-Ca·Mg and HCO₃-Ca·Mg. Strontium is primarily derived from carbonate rocks and celestite-bearing evaporites, whereas metasilicic acid mainly originates from quartz dissolution along the upstream groundwater flow path. PHREEQC-based inverse modeling indicates that, during localized thermal mineral water runoff in the middle-lower reaches or discharge areas, calcite dissolves while dolomite and quartz tend to precipitate, reflecting calcite dissolution-dominated water–rock interactions and near-saturation conditions for some minerals at late runoff stages. Full article

Continuous cropping leads to declines in soil productivity and biodiversity, as well as a deterioration of overall phytosanitary conditions. What if we rotate the intercrops instead of the main crops? In a stationary three-year field experiment, maize was intercropped with Fabaceae (faba bean, crimson and Persian clovers, and blue-flowered alfalfa), Poaceae (winter rye, annual ryegrass, spring barley, and common oat), and Brassicaceae (white mustard, spring oilseed rape, oilseed radish, and spring Camelina) intercrops in separate growing seasons. Fabaceae intercrops developed slowly and competed poorly with weeds. The highest air-dried biomass (ADM) was produced by Persian and crimson clovers (approx. 86 g m⁻²). Intercrops of the Poaceae family, particularly rye and oats, as well as ryegrass, which was the most productive at 200 g m⁻² ADM, germinated faster and competed effectively with weeds. Brassicaceae intercrops also developed rapidly, especially mustard, Camelina, and radish (the most productive 206 g m⁻² ADM). Most intercrops competed with maize and reduced its biomass productivity; however, their competitive effects were weaker than those of weeds. A strong negative correlation between maize and weed biomass was detected (max. r = −0.946; p < 0.01). Complex evaluation index (CEI) showed that the crimson clover–annual ryegrass–spring oilseed rape rotation (CC-AR-SR) was the most productive and was effective in suppressing major weeds Echinochloa crus-galli, Chenopodium album, Polygonum lapathifolium, and Cirsium arvense, less competitive with maize (CEI 4.82), and can be used as an Integrated Pest Management tool. Full article

The intestine is considered a habitat for both bacteria and parasites. In this study, many fecal bacterial isolates and the protozoan Blastocystis sp. were recovered from stool samples of individuals with gastrointestinal conditions. Isolated bacteria were tested for extracellular protease production, and the most potent producer was identified by 16SrDNA gene sequencing as P. aeruginosa FZM498. The enzyme was extracted and purified to electrophoretic homogeneity by the DEAE-Sepharose ion-exchanger and SDS-PAGE revealed a major band at 42.15 KDa. It exhibited maximal activity at 35 °C with thermostability at 60 °C (T_1/2 = 200.04 min). It was most active at pH 8.0 and stable at 5.0–9.5. Enzymatic activity was greatly stimulated in the presence of Fe²⁺ ions, but was repressed by Zn²⁺ and Hg²⁺ ions. Inhibition by PMSF, TLCK, aprotinin, benzamidine, and SBTI protease reagents suggests a serine protease family. The V_max and K_m dynamic constants against azocasein were 36.232 U/mL and 0.0072 mM, respectively. It exhibited the lowest K_m value against the synthetic substrate D-Val-Leu-Lys-pNA among all substrates, indicating a plasmin-like activity. Interestingly, when tested against Blastocystis sp., cysts appeared progressively shrunken, ruptured, and mycelial-like, indicating complete structural collapse with leakage of intracellular contents. The importance of this research is that it is the first study to test the anti-Blastocystis activity of an extracted bacterial serine protease from the gut. This could be a promising, eco-friendly, natural alternative as an anti-Blastocystis agent. The objective of this study was to isolate, purify, and biochemically characterize an extracellular serine protease produced by gut-associated bacteria, as well as to assess its in vitro anti-Blastocystis efficacy as a potential natural and ecologically friendly antiparasitic therapy. Full article

This study was aimed at analyzing physical fitness in 9–11-year-old children and verifying whether it is affected by body mass index (BMI), peak height velocity (PHV), quartile distribution (QD), and sport practice (SP), also considering any potential effects of sex. One thousand one hundred forty-three Italian primary school children (50.7% males) underwent anthropometric measurements (body mass, height, and BMI) and physical tests for measuring coordination (Plate Tapping, PT), handgrip strength (HandGrip, HG), lower-limb power (standing long jump, SLJ), low-back flexibility (sit-and-reach, SR), and sprint (20 m sprint, 20 m) skills. A series of analyses of covariance (ANCOVAs) were conducted using age as a covariate to examine differences among subgroups for BMI, PHV, QD, and SP in relation to the different physical tests (i.e., PT, HG, SLJ, SL, 20 m). Sex was included in each model as fixed independent variable. Principally, participants with higher SP and BMI reported higher and lower performance (p < 0.001) in SLJ, SR, and 20 m tests, respectively. Differently, for higher BMI levels, higher HG performance was reported (p < 0.001). PHV and QD had isolated effects, whereas no effect emerged for PT. Sex interactions were found only for SP subcategories in SR (p ≤ 0.001, ES range = 0.74–1.30). Although physical performance in 9–11-year-old (non-competitive, pre-puberty) Italian students does not seem to be characterized by involuntary factors (such as PHV and QD), substantial opposite trends seem to exist for voluntary factors (such as BMI and SP), thus suggesting how an adequate lifestyle and physical activity could crucially lead to valuable fitness benefits. Full article

The Northwestern Black Sea shelf, strongly influenced by Danube discharge and coastal activities, provides an effective setting for separating lithogenic controls from localized anthropogenic inputs. We applied a multi-proxy geochemical–radiometric approach to Romanian shelf waters and surface sediments. A CTD–Rosette was used to quantify nutrients, chlorophyll-a, TOC, and TN. Dissolved metals and PAHs were measured in seawater, while surface sediments were analyzed for CaCO₃, TOC, trace metals, and γ-emitting radionuclides. Multivariate statistics (PCA/FA) were used to resolve the dominant environmental controls. Summer stratification was characterized by the bottom-layer maxima of PO₄³⁻, SiO₄⁴⁻, and NH₄⁺ and a pronounced subsurface chlorophyll-a maximum at 12–16 m. Surface-water Σ16PAH ranged from 134 to 347 ng L⁻¹ and was dominated by low-molecular-weight compounds, with episodic nearshore enrichment in high-molecular-weight species. In sediments, CaCO₃ ranged from 7.6 to 29.9% and TOC from 0.11 to 0.96%. Trace metals were generally low. Pb and Hg peaked at nearshore station S23, whereas mean Ni (38.88 ppm) slightly exceeded the 35 ppm guideline, consistent with natural Fe/Mn-oxide association. PCA/FA identified a terrigenous axis (Fe-Al-Ti-V-Ni-Cr), a carbonate axis (CaCO₃; Sr where available), and an anthropogenic factor (Pb, Hg, HMW-PAHs). γ-spectrometry provided a compatible radiometric baseline that supports the multi-proxy interpretation. Full article