Search Results (357)

The impact of five different nets—conventional black, grey, white, and photoselective red and yellow—on the performance of “Gala Redlum” apples was evaluated over a five-year period (2020–2024) and compared to an uncovered control. The cumulative production over this period, ranked from highest to lowest, was as follows: white net (182.4 t/ha), grey net (178.5 t/ha), yellow net (175.8 t/ha), black net (175.5 t/ha), red net (169.5 t/ha), and uncovered control (138.8 t/ha). Vegetative growth results were inconsistent among the studied years. The cumulative photosynthetic rate (An) was slightly higher under the white net (57.9 µmol m⁻² s⁻¹). Fv/Fm values remained closest to optimal levels under the black and grey nets. Netting effectively protected fruits from elevated temperatures, particularly under the grey net, and reduced sunburn damage, with the grey, black, and yellow nets performing best in this regard. Overall profitability was increased by netting: the black net provided the highest cumulative income per hectare over a five-year period (EUR 72,315) alongside the second-lowest sunburn loss (0.69%), while the yellow net also showed strong economic performance (€64,742) with a moderate sunburn loss (1.26%) compared to the red net. Fruit dry matter and soluble solids content (SSC) were generally higher in the uncovered control, whereas °Hue values tended to be higher under the red and yellow nets. In summary, the black and yellow nets provided more balanced microclimatic conditions that enhanced tree performance, particularly under heat stress, leading to improved yield and profitability. However, the economic feasibility of each net type should be evaluated in relation to its installation and maintenance costs. Full article

Ultraviolet B (UVB) irradiation drives skin photodamage, prompting exploration of natural therapeutics. This study investigated the reparative effects and mechanisms of crude Gastrodia elata polysaccharides (GP) on UVB-induced acute skin damage. GP was extracted from fresh G. elata via water extraction and alcohol precipitation. It is a homogeneous polysaccharide with a weight-average molecular weight of 808.863 kDa, comprising Ara, Glc, Fru, and GalA. Histopathological analysis revealed that topical application of GP on the dorsal skin of mice effectively restored normal physiological structure, suppressing epidermal hyperplasia and collagen degradation. Biochemical assays showed that GP significantly reduced the activities of MPO and MDA following UVB exposure while restoring the enzymatic activities of SOD and GSH, thereby mitigating oxidative stress. Moreover, GP treatment markedly upregulated the anti-inflammatory cytokines TGF-β and IL-10 and downregulated the pro-inflammatory mediators IL-6, IL-1β, and TNF-α, suggesting robust anti-inflammatory effects. Transcriptomics revealed dual-phase mechanisms: Early repair (day 5) involved GP-mediated suppression of hyper inflammation and accelerated necrotic tissue clearance via pathway network modulation. Late phase (day 18) featured enhanced anti-inflammatory, antioxidant, and tissue regeneration processes through energy-sufficient, low-inflammatory pathway networks. Through a synergistic response involving antioxidation, anti-inflammation, promotion of collagen synthesis, and acceleration of skin barrier repair, GP achieves comprehensive repair of UVB-induced acute skin damage. Our findings not only establish GP as a potent natural alternative to synthetic photoprotective agents but also reveal novel pathway network interactions governing polysaccharide-mediated skin regeneration. Full article

Diabetic kidney disease (DKD) remains the leading cause of end-stage kidney disease (ESKD) globally. Despite advances in our understanding of its pathophysiology, current therapies are often insufficient to stop its progression. In recent years, microRNAs (miRNAs)—small, non-coding RNA molecules involved in post-transcriptional gene regulation—have emerged as critical modulators of key pathogenic mechanisms in DKD, including fibrosis, inflammation, oxidative stress, and apoptosis. Numerous studies have identified specific miRNAs that either exacerbate or mitigate renal injury in DKD. Among them, miR-21, miR-192, miR-155, and miR-34a are associated with disease progression, while miR-126-3p, miR-29, miR-146a, and miR-215 demonstrate protective effects. These molecules are also detectable in plasma, urine, and renal tissue, making them attractive candidates for diagnostic and prognostic biomarkers. Advances in therapeutic technologies such as antagomiRs, mimics, locked nucleic acids, and nanoparticle-based delivery systems have opened new possibilities for targeting miRNAs in DKD. Additionally, conventional drugs, including SGLT2 inhibitors, metformin, and GLP-1 receptor agonists, as well as dietary compounds like polyphenols and sulforaphane, may exert nephroprotective effects by modulating miRNA expression. Recent evidence also highlights the role of gut microbiota in regulating miRNA activity, linking metabolic and immune pathways relevant to DKD progression. Further research is needed to define stage-specific miRNA signatures, improve delivery systems, and develop personalized therapeutic approaches. Modulation of miRNA expression represents a promising strategy to slow DKD progression and improve patient outcomes. Full article

Introduction: Work life quality (WLQ) in nursing is a critical factor that influences both staff well-being and the quality of care provided to patients. Spiritual intelligence (SI) and servant leadership (SL) have been identified as potential positive predictors of WLQ, as they facilitate resilience, job satisfaction, and stress management in highly demanding hospital environments. However, the specific relationship between these constructs in the Peruvian nursing context has not yet been thoroughly explored. Objective: We aimed to examine the impact of spiritual intelligence and servant leadership on the work life quality of Peruvian nurses, assessing their predictive role through a structural equation modeling approach. Methods: A cross-sectional and explanatory study was conducted with a sample of 134 Peruvian nurses (M = 36.29 years, SD = 7.3). Validated Spanish-language instruments were used to measure SI, SL, and WLQ. Structural equation modeling (SEM) with a robust maximum likelihood estimator (MLR) was employed to evaluate the relationships between the variables. Results: Spiritual intelligence showed a positive correlation with WLQ (r = 0.40, p < 0.01) and with servant leadership (r = 0.44, p < 0.01). Likewise, servant leadership had a significant relationship with WLQ (r = 0.53, p < 0.01). The structural model demonstrated a good fit (χ² = 1314.240, df = 970, CFI = 0.96, TLI = 0.96, RMSEA = 0.05, SRMR = 0.08). The hypothesis that SI positively predicts WLQ was confirmed (β = 0.41, p < 0.001), as was the significant effect of SL on WLQ (β = 0.26, p < 0.001). Conclusions: The results indicate that both spiritual intelligence and servant leadership are key predictors of work life quality in Peruvian nurses. SI contributes to developing a transcendent perspective on work and greater resilience, while SL fosters a positive and motivating organizational environment. It is recommended to implement training programs and leadership strategies focused on these constructs to enhance work life quality in the healthcare sector. Full article

Local apple (Malus x domestica Borkh.) cultivars with tolerance to environmental stress factors must be re-evaluated. While the cultivation of apple trees has a long-standing tradition in Hungary, only a handful of cultivars are produced on a large scale, reflecting a trend in global apple production. The most commonly cultivated apple cultivars worldwide include ‘Golden Delicious’, ‘Red Delicious’, ‘Gala’, ‘Fuji’, and ‘Granny Smith’ (with ‘Jonagold’ and ‘Idared’ being significant in Europe). As a result, genetic diversity among apple cultivars has decreased significantly, which has increased the risk of epidemics if a new pathogen appears. Nonetheless, old and local apple cultivars of the Carpathian Basin have adapted well to Hungarian environmental stress factors and pathogens and seem tolerant to them. In this study, fruit analyses and consumer evaluations of eighteen old, local apple cultivars were conducted. Various physicochemical parameters, such as fruit mass, firmness, total soluble solid content, and total acid content, as well as the content of some biological active compounds, including polyphenol content, antioxidant capacity (FRAP), and pectin content, were determined. Additionally, a consumer evaluation was carried out. Based on the results, most of the old, local apple cultivars exhibit high fruit quality and offer considerable health benefits. The proportion of biologically active compounds in these cultivars is equal to or higher than that in the reference cultivar ‘Watson Jonathan’. Based on the excellent fruit quality and consumer preferences, the ‘Harang alma’ (an attractive fruit exhibiting high FRAP values) and ‘Marosszéki piros’ (with firm flesh and a high pectin content and being particularly tasty) cultivars are recommended for backyard gardens. Full article

Background/Objectives: High sodium consumption increases the risk of hypertension and cardiovascular disease. Although food remains the primary source of intake, elevated sodium levels in drinking water can further contribute to excessive intake, particularly in populations already exceeding recommendations. This review examines the extent to which national drinking water standards account for sodium-related health risks and aims to inform discussion on the need for enforceable, health-based sodium limits. Methods: National standards for unbottled drinking water in 197 countries were searched for using the WHO 2021 review of drinking water guidelines, the FAOLEX database, and targeted internet and AI searches. For each country, data were extracted for the document name, year, regulatory body, regulation type, sodium limit (if stated), and rationale. Socio-geographic data were sourced from World Bank Open Data. A descriptive analysis was conducted using Microsoft Excel. Results: Standards were identified for 164 countries. Of these, 20% (n = 32), representing 30% of the global population, had no sodium limit. Among the 132 countries with a sodium limit, 92% (n = 121) adopted the WHO’s palatability-based guideline of 200 mg/L. Upper limits ranged from 50 to 400 mg/L. Only twelve countries (9%) cited health as a rationale. Three countries—Australia, Canada, and the United States—provided a separate recommendation for at-risk populations to consume water with sodium levels below 20 mg/L. Conclusions: Globally, drinking water standards give inadequate attention to sodium’s health risks. Most either lack sodium limits or rely on palatability thresholds that are too high to protect health. Updating national and international standards to reflect current evidence is essential to support sodium reduction efforts. Health-based sodium limits would empower communities to better advocate for safe water. Amid rising water salinity, such reforms must be part of a broader global strategy to ensure universal and equitable access to safe, affordable drinking water as a basic human right. Full article

To study the orbits of satellites, a galaxy can be modeled either by means of a static gravitational potential or by live N-body particles. Analytic potentials allow for fast calculations but are idealized and non-responsive. On the other hand, N-body simulations are more realistic but demand higher computational cost. Our goal is to characterize the regimes in which analytic potentials provide a sufficient approximation and those where N-bodies are necessary. We perform two sets of simulations, using both Gala and Gadget, in order to closely compare the orbital evolution of satellites around a Milky Way-like galaxy. Focusing on the periods when the satellite has not yet been severely disrupted by tidal forces, we find that the orbits of satellites up to ${10}^8{\mathrm{M}}_{\odot }$ can be reliably computed with analytic potentials to within 5% error if they are circular or moderately eccentric. If the satellite is as massive as ${10}^9{\mathrm{M}}_{\odot }$ then errors of 9% are to be expected. However, if the orbital radius is smaller than 30 kpc then the results may not be relied upon with the same accuracy beyond 1–2 Gyr. Full article

This study compares the physicochemical properties of Royal Gala apple snacks fresh and processed using different methods: solar-dried (SD), oven-dried at 65 °C (OD65°) and 85 °C (OD85°) and two commercial brands (CC—commercial apple C and CF—commercial apple F). Evaluated parameters included color, microstructure, acidity, sugar content, phenolic compounds, antioxidant activity, and the presence of heat-induced compounds such as 5-hydroxymethylfurfural (5-HMF) and acrylamide. Commercial samples showed more pronounced color changes and a denser microstructure, with higher browning index (BI) values. The ratio of soluble solids to titratable acidity, an indicator of sensory acceptance, was more influenced by drying methods than temperature. Total phenolic content was highest in fresh apples (123.68 mg GAE/100 g d.m.) and decreased across all drying methods, particularly in solar-dried (SD) samples (78.57 mg GAE/100 g d.m.). Antioxidant activity followed a similar trend, although SD samples performed better than expected, likely due to the retention of certain bioactive compounds. Fresh apples had the highest sugar content (43.25 mg/100 g d.m.), followed by CC (33.81 mg/100 g d.m.), OD65° (33.37 mg/100 g d.m.), CF (31.56 mg/100 g d.m.), OD85° (25.92 mg/100 g d.m.) and SD (25.01 mg/100 g d.m.). Commercial samples were sweeter and darker, with detectable levels of 5-HMF and acrylamide. The findings highlight that drying method significantly affects snack quality. While solar- and oven-dried samples better preserve bioactive compounds, industrial processes enhance sweetness and visual appeal but increase the formation of potentially undesirable compounds. These results are valuable for both consumers and producers aiming to balance sensory quality with nutritional and food safety considerations in dried apple products. Full article

The aim of this study was to investigate the preferences of young adults in the Polish apple market in response to the declining consumption of these fruits. To address the research questions, a study was conducted among young adults using a custom-designed online questionnaire. The research sample consisted of 729 participants. The data were analyzed using ANOVA, and due to the nature of the data, Wilcoxon tests were also employed to examine differences. The most frequently purchased fruits among young adults were bananas and apples; however, strawberries and raspberries were the most favored. The most preferred apple cultivars were bicolored (e.g., Jonagored) and red (e.g., Gala Royal), while yellow cultivars (e.g., Golden Delicious) were perceived as the least attractive. Young consumers favored apples that were juicy, firm, and moderately sweet. This study demonstrated that the skin color of an apple was associated with expectations regarding its firmness and sweetness. Apples with intense coloration (dark red and green) received the highest valuations, particularly when organically grown. In contrast, apples from conventional production systems were valued below their market price by young consumers, indicating the need for strategies aimed at enhancing their perceived value. Full article

Viral infections impose a significant burden on global public health and the economy. This study examines the current state of CRISPR-Cas system research, focusing on their applications in viral detection and their evolution over recent years. A bibliometric analysis and systematic review were conducted using articles published between 2019 and 2024, retrieved from Web of Science, Scopus, and PubMed databases. Out of 2713 identified articles, 194 were included in the analysis. The findings reveal substantial growth in scientific output related to CRISPR-Cas systems, with the United States leading in research and development in this field. The rapid increase in CRISPR-Cas research during this period underscores its immense potential to transform viral diagnostics. With advantages such as speed, precision, and suitability for deployment in resource-limited settings, CRISPR-Cas systems outperform many traditional diagnostic methods. The concerted efforts of scientists worldwide further highlight the promising future of this technology. CRISPR-Cas systems are emerging as a powerful alternative, offering the possibility of expedited and accessible point-of-care testing and paving the way for more equitable and effective diagnostics on a global scale. Full article

Background: Crohn’s disease (CD) is an inflammatory bowel disease in which there is an alteration in the homeostasis and functionality of the intestinal mucosa accompanied by a dysbiosis of the commensal microbiota. The analysis of different dietary strategies to achieve CD remission and reduce gastrointestinal symptoms concludes that it is necessary to restrict the intake of ultra-processed products and to promote the consumption of those with anti-inflammatory effects that improve intestinal permeability and dysbiosis. Methods: Based on previous studies conducted in other cohorts, mainly pediatric, we propose an experimental, prospective, randomized study in patients with active CD who do not show improvement with conventional pharmacological treatment. The control group will receive standard nutritional recommendations while the intervention group will be prescribed an exclusion diet supplemented with enteral nutrition. Results: Patients in the intervention group are expected to exhibit increased lean body mass and reduced visceral fat, as measured by bioelectrical impedance analysis (BIA), alongside higher rates of clinical remission (CDAI), decreased inflammatory markers, and improved gut microbiota composition. Additionally, improvements in health-related quality of life are anticipated, as assessed by validated questionnaires. Conclusions: In the present project, we plan to conduct a detailed study to determine the potential of the exclusion diet for the treatment and remission of CD in adult patients, with the hypothesis that this nutritional intervention will be able to modify and improve intestinal dysbiosis, inflammatory status, and clinical and body composition markers in these patients. Full article

Canning is an excellent solution to provide convenient, affordable, nutritious, and safe seafood with a long shelf life. However, many canned products use tuna, sardines, and Atlantic chub mackerel, species that raise sustainability concerns and whose overuse can put additional pressure on them. Hence, this study aimed to i) develop and assess a nutritious and sustainable canned meal prototype using the Atlantic horse mackerel (Trachurus trachurus) (whose EU allowable-catch amounts have increased, particularly, in Iberian waters) and vegetables in light brine in terms of stability, sterility, and physicochemical and sensory properties over a 4-month period at room temperature and ii) evaluate its nutritional contribution for different population groups. After preparation, the meal was stored for one month at ≈20 °C and ≈40 °C (to simulate the 4 months). Although the pH was not stable, the meal was considered commercially sterile according to the challenge accelerated tests. Moreover, aging did not significantly affect the meal’s physicochemical and sensory properties. This innovative meal prototype can be claimed to be “low-fat”, “reduced in NaCl/Na”, a “source of protein, phosphorus, iron, selenium and vitamin D”, and “high in vitamin B12”. It proved to be both nutritious and appealing for consumption, with potential to be scaled up. Full article

This study explores the acoustic, mechanical and sensory characteristics of Royal Gala dried apples, with a special focus on the potential of solar drying as a sustainable processing method. Apple samples were subjected to different drying techniques, being solar dried (SDA) or oven dried (ODA), with two industrially processed commercial products (CCA—commercial apples C and CFA—commercial apples F) included. The samples were analyzed using acoustic measurements, X-ray diffraction (XRD) and sensory evaluation to assess textural properties and consumer perception. Acoustic analysis revealed that crispier samples produced louder and higher-frequency sounds upon fracture, showing strong alignment with sensory assessments. X-ray diffraction indicated an increase in crystallinity during dehydration, with a shift in the amorphous peak toward lower angles, and reduced intensity, reflecting progressive water removal. Sensory evaluation showed varying degrees of crispiness among the samples, in the following order: CFA > SDA > CCA > ODA. Consumer testing highlighted greater acceptance and consensus for SDA and ODA samples in terms of texture and overall appeal, whereas CCA and CFA received more polarized opinions. These findings demonstrate how different drying methods influence the structural and textural properties of dried apples. Solar drying was shown to be a promising sustainable alternative; as it uses a renewable energy source, it has a low operating cost and simple maintenance. It allows farmers and small producers to process their own food, adding value and reducing post-harvest losses, preserving desirable textural attributes and achieving high consumer acceptance. Full article

Galaxamide, an N-methylated cyclo-pentapeptide containing five D-leucines isolated from Galaxaura filamentosa, has shown significant antitumor activity. This unique cyclo-pentapeptide offered a fresh skeleton for structural modifications. Herein, galaxamide and its 23 analogs (Gala01~Gala24) were designed and synthesized by substituting D-leucine with various proteinogenic amino acids or altering the amino acid configuration using the “3 + 2” strategy, and the in vitro antitumor activity of these cyclopeptides was studied utilizing the CCK-8 assay against two human tumor cell lines (A549 and K562) and one human normal cell line (293T). The total yields of galaxamide and its analogs reached 9.7% and 9.1–16.0%, respectively. CCK-8 assays demonstrated that these compounds showed broad-spectrum antitumor activity, with Gala04 exhibiting outstanding activity against K562 cells (IC₅₀ = 4.2 µM). The anticancer efficacy of galaxamide analogs against tumor cell lines was significantly influenced by the quantity of D-leucines and the D-leucine position. Full article

Background/Objectives: Glaucoma is the leading cause of irreversible blindness worldwide and oxidative stress is considered to play a key role in its development. While antioxidants offer a promising approach to mitigating oxidative stress, their clinical application is often hindered by bioavailability and absorption challenges. Entrapment antioxidants within liposomes may overcome these issues, enhancing their stability and delivery. The aim of this study was to develop a novel composite liposomal formulation for glaucoma treatment, designed to enhance lipoic acid bioavailability and administration through its incorporation into the lipid bilayer. Methods: Liposomes were prepared via lipid film hydration and extrusion. To characterize them, the following analyses were performed: FTIR spectroscopy, liposomal bilayer melting temperature (Tm), TEM, DLS, Z-potential, antioxidant activity, and cytotoxicity assays. Results: The efficient incorporation of lipoic acid into the liposomes’ lipid bilayer was confirmed by FTIR. This incorporation resulted in an increase in the Tm, from 37.0 °C for liposomes to 40.0 °C for liposomes with lipoic acid (L-LA). TEM images confirmed that the spherical morphology of the lipid vesicles remained unchanged following LA incorporation. Dynamic Light Scattering analysis revealed effective diameters of 423 ± 36 nm for L liposomes and 404 ± 62 nm for L-LA liposomes. Notably, the Z-potential shifted from +4.7 ± 0.4 mV (L) to −0.4 ± 0.3 mV (L-LA). Furthermore, L-LA exhibited significant antioxidant activity (31.6 ± 0.4%) compared with L (5.3 ± 0.3%) and biocompatibility, suggesting its potential for therapeutic applications. Conclusions: In summary, biocompatible composite liposomes with antioxidant capacity were successfully developed, resulting in promising candidates for neuroprotective glaucoma therapy. Full article