Search Results (2,167)

Timely and accurate crop yield estimation is vital for food security and management decision-making. Integrating remote sensing with machine learning provides an effective solution. In this study, based on canopy hyperspectral data collected by an ASD FieldSpec 3 handheld spectrometer during the critical growth stages of winter wheat, 18 vegetation indices (VIs) were systematically calculated, and their correlation with yield was analyzed. At the same time, a continuous projection algorithm, Successive Projections Algorithm (SPA), was used to screen the characteristic bands. Recursive Feature Elimination (RFE) was employed to select optimal features from VIs and characteristic spectral bands, facilitating the construction of a multi-temporal fusion feature set. To identify the superior yield estimation approach, a comparative analysis was conducted among four machine learning models: Deep Forest (DF), Support Vector Regression (SVR), Random Forest (RF), and Gaussian Process Regression (GPR). Performance was evaluated using the coefficient of determination (R²), root mean square error (RMSE), and relative root mean square error (rRMSE). Results indicate that the highest correlations between VIs and grain yield were observed during the flowering and grain-filling stages. Independent analysis showed that VIs reached absolute correlations of 0.713 and 0.730 with winter wheat yield during the flowering and grain-filling stages, respectively, while the SPA further identified key bands primarily in the near-infrared and short-wave infrared regions. On this basis, integrating multi-temporal features through RFE significantly improved the accuracy of yield estimation. Among them, the DF model with the fusion of flowering and filling stage features performed best (R² = 0.786, RMSE = 641.470 kg·hm⁻², rRMSE = 15.67%). This study demonstrates that combining hyperspectral data and VIs from different growth stages provides complementary information. These findings provide an effective method for crop yield estimation in precision agriculture. Full article

Background: Coronary heart disease (CHD) remains a leading cause of morbidity and mortality worldwide. Mitochondria-associated endoplasmic reticulum membranes (MAMs) have recently emerged as critical mediators in cardiovascular pathophysiology; however, their specific contributions to CHD pathogenesis remain largely unexplored. Objective: This study aimed to identify and validate MAM-related biomarkers in CHD through integrated analysis of transcriptomic sequencing data and Mendelian randomization, and to elucidate their underlying mechanisms. Methods: We analyzed two gene expression microarray datasets (GSE113079 and GSE42148) and one genome-wide association study (GWAS) dataset (ukb-d-I9_CHD) to identify differentially expressed genes (DEGs) associated with CHD. MAM-related DEGs were filtered using weighted gene co-expression network analysis (WGCNA). Functional enrichment analysis, Mendelian randomization, and machine learning algorithms were employed to identify biomarkers with direct causal relationships to CHD. A diagnostic model was constructed to evaluate the clinical utility of the identified biomarkers. Additionally, we validated the two hub genes in peripheral blood samples from CHD patients and normal controls, as well as in aortic tissue samples from a low-density lipoprotein receptor-deficient (LDLR−/−) atherosclerosis mouse model. Results: We identified 4174 DEGs, from which 3326 MAM-related DEGs (DE-MRGs) were further filtered. Mendelian randomization analysis coupled with machine learning identified two biomarkers, DHX36 and GPR68, demonstrating direct causal relationships with CHD. These biomarkers exhibited excellent diagnostic performance with areas under the receiver operating characteristic (ROC) curve exceeding 0.9. A molecular interaction network was constructed to reveal the biological pathways and molecular mechanisms involving these biomarkers. Furthermore, validation using peripheral blood from CHD patients and aortic tissues from the Ldlr−/− atherosclerosis mouse model corroborated these findings. Conclusions: This study provides evidence supporting a mechanistic link between MAM dysfunction and CHD pathogenesis, identifying candidate biomarkers that have the potential to serve as diagnostic tools and therapeutic targets for CHD. While the validated biomarkers offer valuable insights into the molecular pathways underlying disease development, additional studies are needed to confirm their clinical relevance and therapeutic potential in larger, independent cohorts. Full article

Obesity has reached epidemic proportions, driven by energy imbalance and limited capacity for adaptive thermogenesis. Brown (BAT) and beige adipose tissues dissipate energy through non-shivering thermogenesis (NST), primarily via uncoupling protein-1 (UCP1), making them attractive targets for increasing energy expenditure (EE). The canonical β-adrenergic pathway robustly activates NST in rodents through β3 adrenoceptors; however, translational success in humans has been limited by low β3 expression, off-target cardiovascular effects, and the emerging dominance of β2-mediated signaling in human BAT. Consequently, attention has shifted to non-adrenergic and UCP1-independent mechanisms that offer greater tissue distribution and improved safety profiles. This review examines a broad spectrum of alternative receptors and pathways—including GPRs, TRP channels, TGR5, GLP-1R, thyroid hormone receptors, estrogen receptors, growth hormone, BMPs, sirtuins, PPARs, and interleukin signaling—as well as futile substrate cycles (Ca²⁺, creatine, and glycerol-3-phosphate) that sustain thermogenesis in beige adipocytes and skeletal muscle. Pharmacological agents (natural compounds, peptides, and small molecules) and non-pharmacological interventions (cold exposure, exercise, diet, and time shift) targeting these pathways are critically evaluated. We highlight the translational gaps between rodent and human studies, the promise of multimodal therapies combining low-dose adrenergic agents with non-adrenergic activators, and emerging strategies such as sarco/endoplasmic reticulum calcium ATPase protein (SERCA) modulators and tissue-specific delivery. Ultimately, integrating adrenergic and non-adrenergic approaches holds the greatest potential for safe, effective, and sustainable obesity management. Full article

The development of cross-border hydrogen energy value chains involves complex interactions between technological, regulatory, and logistical subsystems. Static assessment models often fail to capture the dynamic response of these coupled systems to external perturbations. This study addresses this gap by proposing the Dual Carbon Cooperation Index (DCCI), a data-driven framework designed to quantify the synergy efficiency of the China–Korea hydrogen ecosystem. We construct a dynamic state estimation model integrating three coupled dimensions—Technology Synergy, Regulatory Alignment, and Supply Chain Resilience—utilizing an adaptive weighting algorithm (Triple Dynamic Response). Based on multi-source heterogeneous data (2020–2024), the model employs Natural Language Processing (NLP) for vectorizing unstructured regulatory texts and incorporates an exogenous signal detection mechanism (GPR). Empirical results reveal that the ecosystem’s composite synergy score recovered from 0.38 to 0.50, driven by robust supply chain resilience but constrained by high impedance in technological transfer protocols. Crucially, the novel dynamic weighting algorithm significantly reduces state estimation error during high-volatility periods compared to static linear models, as validated by bootstrapping analysis (1000 resamples). The study provides a quantitative engineering tool for monitoring ecosystem coupling stability and proposes a technical roadmap for reducing system constraints through secure IP data architectures and synchronized standard protocols. Full article

Efficient phosphorus recovery is of great significance for sustainable wastewater management and resource recycling. While chemical precipitation is widely used, its effectiveness under complex multi-factor conditions remains challenging to predict and optimize. This study compiled a multidimensional dataset from recent experimental literature, encompassing key operational parameters (reaction time, temperature, pH, stirring speed) and dosages of three metal precipitants (Al³⁺, Ca²⁺, Fe³⁺) to systematically evaluate and benchmark phosphorus recovery performance across these distinct systems, six machine learning algorithms—Random Forest (RF), eXtreme Gradient Boosting (XGBoost), Gaussian Process Regression (GPR), Elastic Net, Artificial Neural Network (ANN), and Partial Least Squares Regression (PLSR)—were developed and cross-validated. Among them, the GPR model exhibited superior predictive accuracy and robustness. ($R^2$ = 0.69, $RMSE$ = 0.54). Beyond achieving high-fidelity predictions, this study advances the field by integrating interpretability analysis with Shapley Additive Explanations (SHAP) and Partial Dependence Plots (PDP). These analyses identified distinct controlling factors across systems: reaction time and pH for aluminum, Ca²⁺ dosage and alkalinity for calcium, and phosphorus loading with stirring speed for iron. The revealed factor-specific mechanisms and synergistic interactions (e.g., among pH, metal dose, and mixing intensity) provide actionable insights that transcend black-box prediction. This work presents an interpretable Machine Learning (ML) framework that offers both theoretical insights and practical guidance for optimizing phosphorus recovery in multi-metal systems and enabling precise control in wastewater treatment operations. Full article

The analysis of ground-penetrating radar data generally relies on the visual identification of structures on selected profiles and their interpretation in terms of buried features. In simple cases, inverse modelling of the acquired data set can facilitate interpretation and reduce subjectivity. These methods suffer from severe restrictions due to antenna resolution limits, which prevent the identification of tiny structures, particularly in forensic, stratigraphic, and engineering applications. Here, we describe a technique to obtain a high-resolution characterization of the underground, based on the forward modelling of individual traces (A-scans) of selected radar profiles. The model traces are built by superposition of Ricker wavelets with different polarities, amplitudes, and arrival times and are used to create reflectivity diagrams that plot reflection amplitudes and polarities versus depth. A thin bed is defined as a layer of higher or lower permittivity relative to the surrounding material, such that the top and bottom reflections are subject to constructive interference, determining the formation of an anomalous peak in the trace (tuning effect). The proposed method allows the detection of ultra-thin layers, well beyond the Rayleigh vertical resolution of GPR antennas. This approach requires a preliminary estimation of the instrumental uncertainty of common monostatic antennas and takes into account the frequency-dependent attenuation, which causes a spectral shift of the dominant frequency acquired by the receiver antenna. Such a quantitative approach to analyzing radar data can be used in several applications, notably in stratigraphic, forensic, paleontological, civil engineering, heritage protection, and soil stratigraphy applications. Full article

Molting is a critical physiological process for the growth and development of Eriocheir sinensis. Any disruption in this process can significantly affect both survival rates and crab quality. The regulatory mechanisms of molting vary across different stages of the molting cycle and remain poorly understood. In this study, ATAC-seq and RNA-seq were combined to identify the integrated differentially expressed genes (IDEGs) in muscle across adjacent stages of the molting cycle. A total of 17, 491, 84, and 491 IDEGs were identified in the comparisons of inter-molt_vs_pre-molt, pre-molt_vs_molt, molt_vs_post-molt, and post-molt_vs_inter-molt stages, respectively. GO enrichment analysis of these IDEGs revealed several key signaling pathways involved in each adjacent molting stage. The GPCR signaling, steroid hormone-mediated signaling, and smoothened signaling pathways were all active across three molting transitions (pre-molt_vs_molt, molt_vs_post-molt, and post-molt_vs_inter-molt). Among them, the GPCR pathway played a dominant role throughout the process. Further structural analysis and RT-qPCR validation identified eight GPCRs involved in molting regulation: GRM7 and moody were specific to the post-molt_vs_inter-molt stage; Kpna6, ADRB2, and SSTR2 were unique to the pre-molt_vs_molt stage; FMRFaR and gpr161 functioned in both post-molt_vs_inter-molt and pre-molt_vs_molt stages; and mth2 was active in both post-molt_vs_inter-molt and molt_vs_post-molt stages. These findings improve the understanding of molting regulation and provide potential targets for further genetic improvement in E. sinensis. Full article

This study presents the application of ground-penetrating radar (GPR) in the investigation of historical metal ore mining sites in the Lower Silesia region of Poland. The paper outlines the principles of the GPR method and details the measurement procedures used during fieldwork. GPR has proven to be an effective, non-invasive tool for identifying inaccessible or previously unknown underground mining structures, such as shafts, tunnels, and remnants of mining infrastructure. This capability is particularly valuable in the context of extensive and complex post-mining landscapes characteristic of Lower Silesia. The research presents findings from selected sites, demonstrating how GPR surveys facilitated the detection and subsequent archaeological exploration of historical workings. In several cases, the method enabled the recovery of access to underground features, which were then subjected to detailed documentation and preservation efforts. Following necessary safety and adaptation measures, some of these sites have been successfully opened to the public as part of regional tourism initiatives. The study confirms the utility of GPR as a key instrument in post-mining archaeology and mining heritage conservation, offering a rapid and reliable means of mapping subsurface structures without disturbing the terrain. Full article

One major challenge in optimizing vehicle underbody structures for blast protection is the trade-off between the high cost of physical tests and the limited accuracy of simulations. We introduce a predictive framework that is co-driven by limited physical measurements and systematically augmented simulation datasets. The main problem arises from the complex components of blast impact signals, which makes it difficult to augment the load signals for finite element simulations when only extremely small sample sets are available. Specifically, a small-scale data-augmentation model within the wavelet domain based on a conditional generative adversarial network (CGAN) was designed. Real-time perturbations, governed by cumulative distribution functions, were introduced to expand and diversify the data representations for enhanced dataset enrichment. A predictive model based on Gaussian process regression (GPR) that integrates physical experimental data with augmented data wavelet characteristics is employed to estimate injury indices, using wavelet scale energies reduced via principal component analysis (PCA) as inputs. Cross-validation shows that this hybrid model achieves higher accuracy than using simulations alone. Through the case study, the model demonstrates that increased hull angle and depth can effectively reduce occupant injury. Full article

Non-destructive evaluation techniques are increasingly recognised as effective alternatives to destructive testing for estimating the compressive strength of lightweight aggregate concrete (LWAC). Among these, ultrasonic pulse velocity (UPV) is a well-established and widely employed method, characterised by its speed, non-invasiveness, and relative simplicity of implementation. In this study, an experimental dataset comprising 640 core segments from 160 cylindrical specimens, provided for analysis, was investigated. Each segment was described by physical and processing variables or features, including lightweight aggregate (LWA) and concrete densities, casting and vibration times, experimental dry density, and P-wave velocity obtained through UPV testing. A segregation index, derived from UPV measurements and defined as the ratio of local to mean P-wave velocity within each specimen, was also considered, following approaches previously suggested in the literature. A range of machine learning techniques was applied to assess the predictive capacity of local P-wave velocity and segregation index. Most ensemble-based methods and support vector regression (SVR) achieved the highest predictive performance when the segregation index was excluded, suggesting that its inclusion did not improve the predictive ability of the models. By contrast, Gaussian process regression (GPR) showed slight improvements when the segregation index was included. The results confirmed that the P-wave velocity measured by UPV testing is a reliable non-destructive predictor of compressive strength in LWAC. At the same time, the added value of the segregation index remained negligible under conditions of low segregation, as reflected by segregation index values above 0.8. These findings highlight the practical potential of integrating UPV-based measurements with data-driven modelling to enhance the reliability of concrete characterisation and quality control. Full article

The detection of underground cavities and dissolution features is a critical component in assessing geohazards within karst terrains, particularly where natural processes interact with long-term human occupation. This study investigates two contrasting sites in the Sefrou region of northern Morocco: Binna, a rural travertine-dolomite system shaped by Quaternary karstification, and the urban Old Medina of Bhalil, where traditional cave dwellings are carved into carbonate formations. A combined geophysical and geological approach was applied to characterize subsurface heterogeneities and assess the extent of near-surface void development. Vertical electrical soundings (VES) at Binna site delineated high-resistivity anomalies consistent with air-filled cavities, dissolution conduits, and brecciated limestone horizons, all indicative of an active karst system. In the Bhalil old Medina site, ground-penetrating radar (GPR) with low-frequency antennas revealed strong reflection contrasts and localized signal attenuation zones corresponding to shallow natural cavities and potential anthropogenic excavations beneath densely constructed areas. Geological observations, including lithostratigraphic logging and structural cross-sections, provided additional constraints on cavity geometry, depth, and spatial distribution. The integrated results highlight a high degree of subsurface karstification across both sites and underscore the associated geotechnical risks for infrastructure, cultural heritage, and land-use stability. This work demonstrates the value of combining electrical and radar methods with geological analysis for mapping hazardous subsurface voids in cavity-prone Quaternary landscapes, offering essential insights for risk mitigation and sustainable urban and rural planning. Full article

Estimating the mass of Oudemansiella raphanipies quickly and accurately is indispensable in optimizing post-harvest packaging processes. Traditional methods typically involve manual grading followed by weighing with a balance, which is inefficient and labor-intensive. To address the challenges encountered in actual production scenarios, in this work, we developed a novel pipeline for estimating the mass of multiple Oudemansiella raphanipies. To achieve this goal, an enhanced deep learning (DL) algorithm for instance segmentation and a machine learning (ML) model for mass prediction were introduced. On one hand, to segment multiple samples in the same image, a novel instance segmentation network named FinePoint-ORSeg was applied to obtain the finer edges of samples, by integrating an edge attention module to improve the fineness of the edges. On the other hand, for individual samples, a novel cap–stem segmentation approach was applied and 18 phenotypic parameters were obtained. Furthermore, principal component analysis (PCA) was utilized to reduce the redundancy among features. Combining the two aspects mentioned above, the mass was computed by an exponential GPR model with seven principal components. In terms of segmentation performance, our model outperforms the original Mask R-CNN; the $\mathrm{A}\mathrm{P}$, ${\mathrm{A}\mathrm{P}}_{50}$, ${\mathrm{A}\mathrm{P}}_{75}$, and ${\mathrm{A}\mathrm{P}}_{\mathrm{s}}$ are improved by 2%, 0.7%, 1.9%, and 0.3%, respectively. Additionally, our model outperforms other networks such as YOLACT, SOLOV2, and Mask R-CNN with Swin. As for mass estimation, the results show that the average coefficient of variation (CV) of a single sample mass in different attitudes is 6.81%. Moreover, the average mean absolute percentage error (MAPE) for multiple samples is 8.53%. Overall, the experimental results indicate that the proposed method is time-saving, non-destructive, and accurate. This can provide a reference for research on post-harvest packaging technology for Oudemansiella raphanipies. Full article

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a widespread hepatic condition characterised by hepatic lipid accumulation and inflammation. Emerging research highlights the contribution of the intestinal microbiota and its metabolic byproducts to the pathogenesis of MASLD through the gut–liver axis. Apolipoprotein A-I (apoA-I), the principal structural component of high-density lipoprotein (HDL), is linked to various metabolic disorders; however, its function in MASLD has not yet been clearly elucidated. This study sought to examine whether apoA-I protects against MASLD, with a focus on the possible role of the gut microbiota and propionic acid (PPA). The contribution of the gut microbiota was evaluated using faecal microbiota transplantation (FMT) and antibiotic cocktail (ABX)-mediated depletion. Microbial composition was assessed via 16S rRNA sequencing, and concentrations of short-chain fatty acids (SCFAs) were quantified. The effects of PPA on MASLD were examined using in vivo and in vitro models. The results showed that apoA-I overexpression alleviated MASLD in a gut microbiota-dependent manner, restored microbial homeostasis, and elevated PPA levels. PPA supplementation improved MASLD phenotypes. Mechanistically, PPA treatment was associated with the activation of the GPR43–Ca²⁺–CAMKII–ATGL pathway, suggesting that PPA plays a role in stimulating hepatic lipolysis and enhancing mitochondrial β-oxidation. These findings reveal a novel pathway through which apoA-I ameliorates MASLD by modulating the gut microbiota and increasing PPA levels, which activate a hepatic lipolysis cascade. The apoA-I–microbiota–PPA axis represents a promising therapeutic target for MASLD intervention. Full article

Precise prediction of structural settlement in subway tunnels is crucial for ensuring safety during both construction and operational phases; however, the non-linear characteristics of monitoring data pose a significant challenge to achieving this goal. To address this issue, this study proposes a hybrid predictive model, termed HO-GPR. This model integrates the Hippopotamus Optimization (HO) algorithm—a novel bio-inspired meta-heuristic—with Gaussian Process Regression (GPR), a non-parametric probabilistic machine learning method. Specifically, HO is utilized to globally optimize the hyperparameters of GPR to enhance its adaptability to complex deformation patterns. The model was validated using 52 months of field settlement monitoring data collected from the Urumqi Metro Line 1 tunnel. Through a series of comparative and generalization experiments, the accuracy and adaptability of the model were systematically evaluated. The results demonstrate that the HO-GPR model is superior to five benchmark models—namely Gated Recurrent Unit (GRU), Support Vector Regression (SVR), HO-optimized Back Propagation Neural Network (HO-BP), standard GPR, and ARIMA—in terms of accuracy and stability. It achieved a Coefficient of Determination (R²) of 0.979, while the Root Mean Square Error (RMSE), Mean Absolute Error (MAE), and Mean Absolute Percentage Error (MAPE) were as low as 0.318 mm, 0.240 mm, and 1.83%, respectively, proving its capability for effective prediction with non-linear data. The findings of this research can provide valuable technical support for the structural safety management of subway tunnels. Full article

Electrical discharge machining (EDM) is widely used for machining hard and difficult-to-cut materials; however, the complex and nonlinear nature of the process makes the accurate prediction of key performance indicators challenging, particularly when only limited experimental data are available. In this study, a combined Taguchi design and Gaussian process regression (GPR) modeling framework is proposed to predict the surface roughness (Ra), material removal rate (MRR), and overcut (OC) in die-sinking EDM. An L18 Taguchi orthogonal array was employed to efficiently design experiments involving discharge current, pulse duration, and electrode material. GPR models with an automatic relevance determination (ARD) radial basis function kernel were developed to capture nonlinear relationships and varying parameter relevance. Model performance was evaluated using strict leave-one-out cross-validation (LOOCV). The developed GPR models achieved low prediction errors, with RMSE (MAE) values of 0.54 µm (0.41 µm) for R_a, 1.56 mm³/min (1.21 mm³/min) for MRR, and 0.0065 mm (0.0055 mm) for OC, corresponding to approximately 9.8%, 5.4%, and 5.9% of the respective response ranges. These results confirm stable and reliable predictive accuracy within the investigated parameter domain. Based on the validated surrogate models, multi-objective optimization was performed to identify Pareto-optimal process conditions, revealing graphite electrodes as the dominant choice within the feasible operating region. The proposed approach demonstrates that accurate and robust prediction of EDM performance can be achieved even with compact experimental datasets, providing a practical tool for process analysis and optimization. Full article