Search Results (3,089)

Discovering, analyzing, and finding a key to understanding the physiological and biochemical responses that Vitis vinifera L. undertakes against drought stress is of fundamental importance for this profitable crop. Today’s considerable climatic fluctuations force researchers and farmers to focus on this issue with solutions inclined to respect the ecosystem. In this academic work, we focused on describing the drought stress consequences on several parameters of secondary metabolites on Vitis vinifera leaves (quercetins, kaempferol, resveratrol, proline, and xanthophylls) and on some ecophysiological characteristics (e.g., water potential, stomatal conductance, and leaf temperature) to compare the answers that diverse agronomic management techniques (i.e., irrigation with and without zeolite, pure zeolite and no application) could instaurate in the metabolic pathway of this important crop with the aim to find convincing and thought-provoking responses to use this captivating and versatile mineral, the zeolite known as the “magic rock”. Stressed grapevines reached 56.80 mmol/m²s gs at veraison and a more negative stem Ψ (+10.63%) compared to plants with zeolite. Resveratrol, in the hottest season, fluctuated from 0.18–0.19 mg/g in zeolite treatments to 0.37 mg/g in stressed vines. Quercetins were inclined to accumulate in response to drought stress too. In fact, we recorded a peak of quercetin (3-O-glucoside + 3-O-glucuronide) of 11.20 mg/g at veraison in stressed plants. It is interesting to note how the pool of metabolites was often unchanged for plants treated with zeolite and for plants treated with water only, thus elevating this mineral to a “stress reliever”. Full article

Interpreters who translate for refugees are regularly confronted with traumatic content. Listening regularly to and translating potentially traumatizing stories make interpreters vulnerable to secondary traumatization. The current study aimed to investigate secondary traumatic stress (STS) in interpreters working with refugees and to identify potential risk and protective factors. In this cross-sectional study, 64 interpreters from Austria, Germany, and Switzerland participated. STS, compassion satisfaction, burnout, and resilience were assessed using the Secondary Traumatic Stress Scale, Professional Quality of Life Scale, and Connor–Davidson Resilience Scale. Sociodemographic data were collected (e.g., experiences with translation in psychotherapy, personal backgrounds of forced displacement, and personal experiences with psychotherapy as a client). Subgroup comparisons were conducted to identify risk factors for STS. A total of 43 participants (67%) showed at least mild STS. STS was significantly associated with burnout. Personal experiences of forced displacement, gender, and working context had no impact on STS. Interpreters with personal experiences of psychotherapy showed a higher level of STS as well as higher resilience than those without personal experiences of psychotherapy. Independent of personal experiences of forced displacement, gender, and working context, interpreters who work with refugees are at high risk of STS. Regular training and supervision for interpreters who work with refugees should thus be offered as standard practice. Full article

Erythrocyte (RBC) aging involves significant structural and nanomechanical alterations crucial to their function. This study aims to bridge the gap between analyses based on statistical morphometric parameters, e.g., membrane roughness, and those based on point-dependent nanomechanical properties, e.g., stiffness or Young’s modulus. Using Atomic Force Microscopy, we investigated morphology, membrane roughness, and nanomechanical properties on the very same RBCs under dehydrated (air) and hydrated (physiological buffer) conditions. The cells were studied at different stages of in vitro aging: one, seven, and 12 days. Our results quantitatively show that across dehydration, as well as along the aging pathway, RBCs become progressively more rigid while their membrane roughness decreases, a trend observed in both environments. Notably, the differences between the hydrated and dehydrated states were large in young cells but diminished when erythrocytes aged. Despite these parallel trends, high-resolution mapping on the nanoscale revealed that roughness and Young’s modulus do not correlate, indicating that these parameters are linked to different properties. In conclusion, this work provides a comprehensive protocol for a biophysical description of RBC aging and establishes that the simultaneous measurement of membrane roughness and nanomechanical properties offers a complementary approach, yielding a more complete characterization of cellular properties. Full article

The aim of this study was to compare the forces and moments exerted by thermoformed aligners (TFMs) and direct printed aligners (DPAs) on the maxillary left central incisor (21) and adjacent teeth (11, 22) during lingual bodily movement of tooth 21. Methods: An in vitro setup was used to quantify forces and moments on three incisors, which were segmented and fixed onto multi-axis force/moment transducers. TFM were fabricated using 0.76 mm-thick single-layer PET-G foils (ATMOS; American Orthodontics, Sheboygan, WI, USA) and multi-layer TPU foils (Zendura FLX; Bay Materials LLC, Fremont, CA, USA). DPAs were fabricated using TC-85 photopolymer resin (Graphy Inc., Seoul, Republic of Korea). Tooth 21 was planned for bodily displacement by 0.25 mm and 0.50 mm, and six force and moment components were measured on it and the adjacent teeth. Results: TC-85 generated lower forces and moments with fewer unintended forces and moments on the three teeth. TC-85 exerted 0.99 N and 1.53 N of mean lingual force on tooth 21 for 0.25 mm and 0.50 mm activations, respectively; ATMOS produced 3.82 N and 7.70 N, and Zendura FLX produced 3.00 N and 8.23 N of mean lingual force for the same activations, respectively. Bodily movement could not be achieved. Conclusions: The force systems generated by clear aligners are complex and unpredictable. DPA using TC-85 produced lower, more physiological force levels with fewer side effects, which may increase the predictability of tooth movement and enhance treatment outcome. The force levels generated by TFM were considered excessive and not physiologically compatible. Full article

In this study, we report an effective, one-step chemical treatment to directly isolate carboxylated cellulose nanocrystals (CCNCs) from a lignocellulosic source using a mixture of peracetic acid and 10% H₂SO₄ solution. We used infrared spectroscopy, X-ray diffraction, dynamic light scattering, atomic force microscopy, and scanning electron microscopy to characterize all the materials. The obtained CCNCs exhibited needle-like shapes with a width of 10–50 nm and a length of 200–500 nm, a high crystalline index (71.3%), and a high content of -COOH groups (~1.405 mmol/g), with a zeta potential value of −48.5 mV. We attributed this to the cooperative effect of strong oxidative agent and strong acid, which makes the removal of all components occur simultaneously in parallel with the partial hydrolysis of amorphous cellulose regions. Our study opens a new, simple approach to directly isolate cellulose nanocrystals from a lignocellulosic source. Full article

Pain following orthodontic treatment is the chief complaint of patients undergoing this form of treatment. Although the use of diode lasers has been suggested for pain reduction, the mechanism of laser-induced analgesic effects remains unclear. Neuropeptides, such as substance P (SP) and calcitonin gene-related peptide (CGRP), contribute to the transmission and maintenance of inflammatory pain. Heat shock protein (HSP) 70 plays a protective role against various stresses, including orthodontic forces. This study aimed to examine the effects of diode laser irradiation on neuropeptides and HSP 70 expression in periodontal tissues induced by experimental tooth movement (ETM). For inducing ETM for 24 h, 50 g of orthodontic force was applied using a nickel–titanium closed-coil spring to the upper left first molar and the incisors of 20 male Sprague Dawley rats (7 weeks old). The right side without ETM treatment was considered the untreated control group. In 10 rats, diode laser irradiation was performed on the buccal and palatal sides of the first molar for 90 s with a total energy of 100.8 J/cm². A near-infrared (NIR) laser with a 808 nm wavelength, 7 W peak power, 560 W average power, and 20 ms pulse width was used for the experiment. We measured the number of facial groomings and vacuous chewing movements (VCMs) in the ETM and ETM + laser groups. Immunohistochemical staining of the periodontal tissue with SP, CGRP, and HSP 70 was performed. The number of facial grooming and VCM periods significantly decreased in the ETM + laser group compared to the ETM group. Moreover, the ETM + laser group demonstrated significant suppression of SP, CGRP, and HSP 70 expression. These results suggest that the diode laser demonstrated analgesic effects on ETM-induced pain by inhibiting SP and CGRP expression, and decreased HSP 70 expression shows alleviation of cell damage. Thus, although further validation is warranted for human applications, an NIR diode laser can be used for reducing pain and neuropeptide markers during orthodontic tooth movement. Full article

The demand for wild animal meat, popularly called “bushmeat”, serves as a driving force behind the emergence of infectious diseases, potentially transmitting a variety of pathogenic bacteria to humans through handling and consumption. This study investigated the microbial load and bacterial diversity in bushmeat sourced from a prominent bushmeat market in Kumasi, Ghana. Carcasses of 61 wild animals, including rodents (44), antelopes (14), and African civets (3), were sampled for microbiological analysis. These samples encompassed meat, intestines, and anal and oral swabs. The total aerobic bacteria plate count (TPC), Enterobacteriaceae count (EBC), and fungal counts were determined. Bacterial identification was conducted using MALDI-TOF biotyping. Fungal counts were the highest across all animal groups, with African civets having 11.8 ± 0.3 log₁₀ CFU/g and 11.9 ± 0.2 log₁₀ CFU/g in intestinal and meat samples, respectively. The highest total plate count (TPC) was observed in rodents, both in their intestines (10.9 ± 1.0 log₁₀ CFU/g) and meat (10.9 ± 1.9 log₁₀ CFU/g). In contrast, antelopes exhibited the lowest counts across all categories, particularly in EBC from intestinal samples (6.1 ± 1.5 log₁₀ CFU/g) and meat samples (5.6 ± 1.2 log₁₀ CFU/g). A comprehensive analysis yielded 524 bacterial isolates belonging to 20 genera, with Escherichia coli (18.1%) and Klebsiella spp. (15.5%) representing the most prevalent species. Notably, the detection of substantial microbial contamination in bushmeat underscores the imperative for a holistic One Health approach to enhance product quality and mitigate risks associated with its handling and consumption. Full article

This literature review provides an overview of the food safety and toxicological characteristics of various heavy metals and metalloids and the public health significance of their occurrence in food. Metals also occur as natural components of the environment, but they can enter food of animal origin and the human body primarily due to anthropogenic (industrial, agricultural, transport-related) activities. The persistent heavy metals (e.g., Hg, Pb, Cd) found in the environment are not biodegradable, can accumulate, and can enter the bodies of higher animals and subsequently, humans, where they are metabolized into various compounds with differing toxicity. Thus, due to their environmental contamination, they can accumulate in living organisms and their presence in the food chain is of great concern for human health. Regulations of the European Community in force lay down maximum levels for a limited number of metals, and the types of regulated foodstuffs of animal origin are also narrower than in the past, e.g., wild game animals and eggs are not included. The regulation of game meat (including offal) deserves consideration, given that it is in close interaction with the environmental condition of a given area and serves as indicator of it. Full article

Channels with three standard symmetrical sections and one asymmetric section are mounted as cantilever beams with the web oriented vertically. A classical solution to the analysis of stress in each thin-walled cantilever channel is provided using the principle of wall shear flow superposition. The latter is coupled with a further superposition between axial stress arising from bending and from the constraint placed on free warping imposed at the fixed end. Closed solutions for design are tabulated for the net shear stress and the net axial stress at points around any section within the length. Stress distributions thus derived serve as a benchmark structure for alternative numerical solutions and for experimental investigations. The conversion of the transverse free end-loading applied to a thin-walled cantilever channel into the shear and axial stress that it must bear is outlined. It is shown that the point at which this loading is applied within the cross-section is crucial to this stress conversion. When a single force is applied to an arbitrary point at the free-end section, three loading effects arise generally: bending, flexural shear and torsion. The analysis of each effect requires that this force’s components are resolved to align with the section’s principal axes. These forces are then considered in reference to its centroid and to its shear centre. This shows that axial stress arises directly from bending and from the constraint imposed on free warping at the fixed end. Shear stress arises from flexural shear and also from torsion with a load offset from the shear centre. When the three actions are combined, the net stresses of each action are considered within the ability of the structure to resist collapse from plasticity and buckling. The novelty herein refers to the presentation of the shear flow calculations within a thin wall as they arise from an end load offset from the shear centre. It is shown how the principle of superposition can be applied to individual shear flow and axial stress distributions arising from flexural bending, shear and torsion. Therein, the new concept of a ‘trans-moment’ appears from the transfer in moments from their axes through centroid G to parallel axes through shear centre E. The trans-moment complements the static equilibrium condition, in which a shift in transverse force components from G to E is accompanied by torsion and bending about the flexural axis through E. Full article

The integration of functional ingredients into 3D food printing formulations presents both opportunities and challenges, particularly regarding the printability and structural integrity of the final product. This study investigates the effect of incorporating omega-3 fatty acids encapsulated in pea protein into a model food gel composed of gelatin and iota-carrageenan. Four formulations with varying concentrations of encapsulated omega-3 (0%, 3%, 3.75%, and 6%) were evaluated for their rheological, textural, and printability properties. Rheological analysis revealed a progressive increase in storage modulus (G′) from 1200 Pa (0%) to 2000 Pa (6%), indicating enhanced elastic behavior. Extrusion analysis showed a reduction in maximum extrusion force from 325 N (0%) to 250 N (6%), and an increase in buffer time from 390 s to 500 s. Print fidelity at time 0 showed minimal deviation in the checkerboard geometry (area deviation: −12%), while the concentric cylinder showed the highest stability over 60 min (height deviation: 9%). These findings highlight the potential of using encapsulated bioactive compounds in 3D food printing to develop functional foods with tailored nutritional and mechanical properties. Full article

The vast majority of photocatalysts find it difficult to consistently and stably exhibit high performance due to the variability of sunlight intensity within a day, as well as the high energy consumption of artificial light sources. In this study, mechanoluminescent Sr₂MgSi₂O₇:Eu,Dy phosphors is combined with NiS@g-C₃N₄ composite to construct a ternary heterogeneous photocatalytic system, denoted as NCS. In addition to the enhanced separation efficiency of photogenerated charge carriers by the formation of a heterojunction, the introduction of Sr₂MgSi₂O₇:Eu,Dy provides an ultra-driving force for the photocatalytic reactions owing to its mechanoluminescence-induced excitation. Results show that the degradation rate of RhB increased significantly in comparison with pristine g-C₃N₄ and NiS@g-C₃N₄, indicating the obvious advantages of the ternary system for charge separation and migration. Moreover, the additional photocatalytic activity of NCS under ultrasound stimulation makes it a promising all-weather photocatalyst even in dark environments. This novel strategy opens up new horizons for the synergistic combination of light-driven and ultrasound-driven heterogeneous photocatalytic systems, and it also has important reference significance for the design and application of high-performance photocatalysts. Full article

Energy harvesting systems (EHSs) are widely used to power wireless sensors. Piezoelectric harvesters have the advantage of producing an electric signal directly related to the exciting force and can thus be used to power condition monitoring sensors in dynamically loaded structures such as bridges. The need for such monitoring is exemplified by the fact that the condition of close to 25% of public roadway bridges in, e.g., Germany is not satisfactory. Stack-based piezoelectric energy harvesting systems (pEHSs) installed near bridge bearings could provide information about the traffic and dynamic loads on the one hand and condition-dependent changes in the bridge characteristics on the other. This paper presents an approach to co-optimizing the design of the mechanical and electrical components using a nonlinear solver. Such an approach has not been described in the open literature to the best of the authors’ knowledge. The mechanical excitation is estimated through a finite element simulation, and the electric circuitry is modeled in Simulink to account for the nonlinear characteristics of rectifying diodes. We use real traffic data to create statistical randomized scenarios for the optimization and statistical variation. A main result of this work is that it reveals the strong dependence of the energy output on the interaction between bridge, harvester, and traffic details. A second result is that the methodology yields design criteria for the harvester such that the energy output is maximized. Through the case study of an actual middle-sized bridge in Germany, we demonstrate the feasibility of harvesting a time-averaged power of several milliwatts throughout the day. Comparing the total amount of harvested energy for 1000 randomized traffic scenarios, we demonstrate the suitability of pEHS to power wireless sensor nodes. In addition, we show the potential sensory usability for traffic observation (vehicle frequency, vehicle weight, axle load, etc.). Full article

Psychological stress and dietary behavior are interdependent forces that greatly influence mental and physical health. Thus, both what and how we eat impact our well-being. Maladaptive eating patterns, such as eating in response to emotional cues rather than physiological hunger, have become increasingly common amid modern stressors and an ultra-processed food environment. This narrative review synthesizes interdisciplinary findings from nutritional psychiatry, microbiome science, and behavioral nutrition to explore how stress physiology, gut–brain interactions, and dietary quality shape emotional regulation and eating behavior. It highlights mechanisms (e.g., HPA-axis dysregulation, blunted interoception, and inflammatory and epigenetic pathways) and examines the evidence for mindful and intuitive eating; phytochemical-rich, whole-food dietary patterns; and the emerging role of precision nutrition. Trauma-informed approaches, cultural foodways, structural barriers to healthy eating, and clinical implementation strategies (e.g., interprofessional collaboration) are considered in the context of public health equity to support sustainable mental wellness through dietary interventions. Ultimately, restoring a healthy relationship with food positions nutrition not only as sustenance but as a modifiable regulator of affect, cognition, and stress resilience, central to mental and physical well-being. Full article

This research investigated the impact of corn–soybean meal-based fermented feed on the growth performance, pork quality, and fatty acid profiles of lean-type finishing pigs. A total of 80 lean-type growing DLY (Duroc × Landrace–Yorkshire) pigs were randomly assigned to 2 groups, with 5 replicates of 8 pigs per pen. The pigs in control group (CON group) were fed a basal diet, while the pigs in fermented feed group (FF group) were fed a diet supplemented with 10% fermented feed. The experimental period lasted 70 days. Results exhibited that pigs in FF group had a significant increase in final body weight and average daily gain (ADG) (p < 0.05) and had a significant decrease in the feed-to-gain ratio (F/G) (p < 0.05). The FF group also exhibited significant promotion in muscle intramuscular fat content, marbling score, and meat color and significantly reduced the meat shear force and drip loss (p < 0.05). Serum analysis indicated that fermented feed significantly elevated blood glucose, total cholesterol, triglyceride levels, and serum hormones such as insulin, leptin, and IGF-1 (p < 0.05). Additionally, fermented feed significantly elevated the levels of polyunsaturated fatty acids (PUFAs) and monounsaturated fatty acids (MUFAs), whereas it decreased the saturated fatty acids (SFAs) contents (p < 0.05). The fermented feed also significantly enhanced pork nutritional values (p < 0.05). The fermented feed increased the expression of IGF-1, SREBP1c, PDE3, PPARγ, SCD5, and FAT/CD36 mRNA (p < 0.05). Furthermore, microbial 16S rDNA analysis uncovered that FF supplementation significantly reduced the Campilobacterota phylum abundance, while increasing the genus abundances of Clostridium_sensu_stricto, norank_f_Oscillospiraceae, unclassified_c_Clostridia, and V9D2013 (p < 0.05). In summary, the results indicated that the microbial fermented feed exhibited the regulation effects on pork quality and nutritional values of lean-type pigs through regulating lipid metabolism and gut microbial composition. Full article

Calcium microcapsules were developed by spray-drying using mannoproteins (MPs) extracted from brewer’s spent yeast, xanthan gum (XG), and maltodextrin as encapsulating materials. The formulas included 11 g of calcium, 24 g of MP, and 0, 2, 4, or 8 g of XG 100 g⁻¹ solids, obtaining C1, C2, C3, and C4 microcapsules, respectively. Maltodextrin was added to complete 100 g of solids. Calcium intestinal (IB), colonic (CB), and total bioaccessibility (TB) were estimated after a simulated gastrointestinal digestion followed by in vitro colonic fermentation. The macromolecules of microcapsules interacted by ionic and hydrophobic forces. Microcapsules C1 and C2 showed a spherical shape. However, the addition of XG to the formulation contributed to the formation of concavities in the microcapsules. All microcapsules had higher IB than the control (CaCl₂), probably due to the calcium-chelating peptides dialyzed from MP. Moreover, C1 and C2 showed the highest IB values (≈23%). However, C3 and C4 showed the highest CB values (≈11%), attributing this effect to the short-chain fatty acids produced during colonic fermentation. Finally, C1 and C2 showed the highest TB (31.8 ± 0.1 and 32.0 ± 0.4%, respectively). The use of MP allowed for obtaining a supplement with high calcium bioaccessibility. Full article