Search Results (131)

As the body of knowledge on feline mammary tumors (FMTs) continues to grow, their histological classification and grading systems have undergone revisions and updates to better reflect the biological behavior of these tumors. In this review, the historical evolution of these frameworks is traced and later revisited in the context of their prognostic relevance. Numerous studies have investigated clinicopathological prognostic factors in feline mammary carcinomas (FMCs); however, the heterogeneity in assessment methods, inclusion criteria for survival analysis, and the clinical endpoints considered can often complicate direct comparisons across different studies and may contribute to seemingly conflicting results. Furthermore, the small cohort size of many studies limits the robustness and transferability of their findings. This paper provides an updated overview of the epidemiological, clinical, and pathological prognostic factors of these tumors, while also highlighting current challenges, methodological limitations, and areas for future improvement. Full article

Feline mammary carcinoma (FMC) exhibits aggressive behavior, with limited treatment options. Given the relevance of the PD-1/PD-L1/PD-L2 axis in human breast cancer immunotherapy, this study assessed PD-1 and its ligands in rare FMC histotypes (n = 48) using immunohistochemistry on tumor cells (TCs), intratumoral lymphocytes (iTILs), and stromal tumor-infiltrating lymphocytes (sTILs). PD-1 was expressed in 13% of TCs, 85% of iTILs, and 94% of sTILs, while PD-L1 was observed in 46% of TCs, 96% of iTILs, and 100% of sTILs. PD-L2 was expressed in 79% of TCs and 100% of both iTILs and sTILs, with PD-L1/PD-L2 co-expression in 42% of TCs. Higher PD-1 IHC scores in TCs were associated with a less aggressive biological behavior; PD-L1 in iTILs was linked to skin ulceration, whereas PD-L2 in TCs was associated with its absence. Our findings highlight the relevance of the PD-1/PD-L1/PD-L2 immune checkpoint in rare FMC subtypes and support further investigation into checkpoint-blockade therapies. Full article

FMC63-CAR T cell therapy targeting CD19 protein on malignant B-cells is effective in patients with relapsed or refractory diffuse large B-cell lymphoma (r/r DLBCL), with complete response rates of 43–54%. Common germline variants of the immune-checkpoint regulator CTLA-4 may elicit different responses to CAR-T cell therapy. The CTLA4 gene single-nucleotide polymorphism rs231775 coding threonine or alanine at amino acid position 17 of the CTLA-4 protein was prevalent in 55% of the studied DLBCL patients. In a retrospective comparative analysis of clinical outcome, there were significant differences in CTLA4 A17hom vs. T17Ahet and T17hom carriers with four-year progression-free survival at 77%, 59%, and 30% (p = 0.019), four-year overall survival was 79%, 41%, and 33% (p = 0.049), the relapse rates were 20%, 37%, and 56% (p = 0.025), and the death rates 20%, 54%, and 52% (p = 0.049). Conclusions: CTLA4 rs231775 polymorphism may impact the treatment outcome in FMC63-anti-CD19 CAR-T cell therapy, with an association of the CTLA4 minor allele A17 to favorable treatment outcome. Full article

Selecting the most suitable machines to use for wood recovery is essential for computing the operating costs of the whole forestry machinery chain (FMC). Nevertheless, a generalized approach for selecting the most suitable FMC and quantifying the corresponding economic performances for wood recovery (i.e., harvesting and long-distance transport) is still missing. The primary aim of this study is to describe a decision support model, called FOREstry MAchinery chain selection (“FOREMA v1”), which is able to (i) select the most feasible FMC and (ii) calculate the costs (such as EUR∙h⁻¹; EUR∙t⁻¹ of dry matter, DM) of each operation (OP) comprising the FMC. The model is made up of three different modules (Ms): machinery chain selection (M1), machinery chain organization (M2), and cost calculation (M3). In M1, feasible FMCs are defined according to seven technical parameters that characterize the forest area. For each FMC, FOREMA v1 defines the sequence of OPs and the types of machines that can potentially be used. Once the characteristics of the area in which wood recovery occurs are processed, the user selects the types of machines to use according to the model’s suggestions. In M2 and M3, the user is supported in organizing the FMC (e.g., calculation of the required time, working productivity, and so on) and computing the operating costs. The secondary aim of this study is to discuss a case study focused on chips production for energy generation, providing empirical evidence on how FOREMA v1 works. The proposed model provides a systematic approach for the selection and optimization of the most suitable FMC to adopt for biomass recovery, thus supporting decision-making processes. The results showed that felling had the lowest cost per unit of time (63.7 EUR·h⁻¹) but the highest cost per unit of mass (35.4 EUR·t DM⁻¹) due to its longer working time and lower productivity. Loading and long-distance transport incurred the highest costs both per unit of time (223.5 EUR·h⁻¹) and per unit of mass (29.4 EUR·t DM⁻¹), attributed to the use of medium–small-sized trailers coupled with tractors operating at low speeds, leading to a high number of cycles. For the entire FMC the costs were equal to 147.3 EUR·h⁻¹ and 101.1 EUR·t DM⁻¹. Full article

High-performance discontinuous carbon-fiber-reinforced thermoplastics (CFRTPs) offer promising manufacturing flexibility and recyclability for advanced composite applications. However, their mechanical performance and reliability strongly depend on the internal fiber architecture, which is largely determined by the molding process. In this study, three distinct compression molding approaches—CFRTP sheet molding compounds (SMCs), bulk molding compounds (BMCs), and free-edge molding compounds (FMCs)—were systematically evaluated to investigate how processing parameters affect fiber orientation, tape deformation, and impregnation quality. X-ray micro-computed tomography (XCT) was employed to visualize and quantify the internal structures of each material, focusing on the visualization and quantification of in-plane and out-of-plane fiber alignment and other internal structure features. The results indicate that CFRTP-SMC retains largely intact tape layers and achieves better impregnation, leading to more uniform and predictable internal geometry. Although CFRTP-BMC exhibits greater tape deformation and splitting due to increased flow, its simpler molding process and better tolerance for tape shape distortion suggest potential advantages for recycled applications. In contrast, CFRTP-FMC shows significant tape fragmentation and poor impregnation, particularly near free edges. These findings underscore the critical role of a controlled molding process in achieving a consistent internal structure for these materials for the first time. This study highlights the utility of advanced XCT methods for optimizing process design and advancing the use of high-performance discontinuous CFRTP in industry. Full article

Background: Hospital infrastructures account for a significant portion of healthcare expenditures, yet the factors driving facility management costs (FMCs) remain underexplored, particularly in the Italian context. This study aims to analyze FMCs in hospitals, focusing on utility and maintenance expenses, while providing benchmarking values to support decision making. Methods: This study employed a mixed-methods approach, integrating a literature review, financial data analysis, and a case study of 27 hospital facilities in Lombardy. Data on utility and maintenance costs were collected from financial statements and supplemented with targeted questionnaires to enhance precision. Descriptive statistics and parametric cost indicators (e.g., EUR/sqm, EUR/bed) were analyzed to identify trends and disparities. Results: FMC increased by an average of 32.90% between 2019 and 2022, with utility expenses constituting 77.45% of total costs and maintenance accounting for 22.45%. Utility costs rose significantly (37.34%), driven by energy and cleaning services, while maintenance costs grew more moderately (18.66%). Cost variability was evident across hospital typologies, with Basic Healthcare Centers averaging 122.86 EUR/sqm compared to 232.66 EUR/sqm for Level II Emergency Hospitals. Conclusions: This study highlights significant variability in FMCs across Italian hospitals and underscores the need for benchmarking to optimize resource allocation. Future research should expand the dataset, incorporating extraordinary maintenance costs, and examine management models to enhance cost efficiency. These findings provide actionable insights for policymakers and healthcare administrators to improve hospital infrastructure sustainability and efficiency. Full article

This study successfully demonstrates the synthesis of foxtail millet carbon-activated (FMCA) materials using a two-step carbonization process from foxtail millet husk (FMH). The pre-carbonized biomass-derived millet husk was chemically activated with KOH at 500 °C and subsequently carbonized in an inert argon atmosphere at 800 °C in a tubular furnace. XRD analysis revealed a diffraction peak at 2θ = 23.67°, corresponding to the (002) plane, indicating the presence of graphitic structures. The Raman analysis of FMCA materials showed an intensity ratio (I_G/I_D) of 1.13, signifying enhanced graphitic ordering and structural stability. The as-prepared FMC and FMCA electrode materials demonstrate efficient charge storage electrochemical symmetric devices. Electrochemical analysis revealed the charge–discharge curves and a specific capacitance of Csp (FMC//FMC) 55.47 F/g and (FMCA//FMCA) 82.94 F/g at 0.5 A/g. Additionally, the FMCA//FMCA symmetric device exhibits superior performance with a higher capacity retention of 94.89% over 5000 cycles. The results confirm the suitability of FMCA for energy storage applications, particularly in electrochemical double-layer capacitors (EDLCs), making it a promising material for next-generation supercapacitors. Full article

Hydropower is a clean and renewable energy source, and cascade hydropower stations have been developed to enhance water energy utilization efficiency. While small hydropower stations have a smaller scale and environmental impact compared to large ones, the cumulative effects of cascade development on river ecosystems should not be overlooked. In this study, flow cytometry was used to classify picoplankton from water samples collected at four small cascade hydropower stations on a Pearl River tributary into six microbial groups: Virus, LNA (Low Nucleic Acid), HNA (High Nucleic Acid), Cyanobacteria, Algae, and Fungi. Four ecological assessment indices were calculated: Photosynthetic Autotrophic Capacity (PAC), Bacterial Activity Index (BAI), Virus Regulatory Capacity (VRC), and Fungal Metabolic Capacity (FMC). By analyzing trends in microbial abundance and ecological indices and their correlations with environmental factors, the results showed that along the small cascade hydropower stations, dissolved oxygen (DO) and electrical conductivity (EC) increased from 5.71 mg/L and 49.87 μS/cm upstream to 6.80 mg/L and 56.18 μS/cm downstream, respectively. In contrast, oxidation-reduction potential (ORP) and total organic carbon (TOC) concentrations decreased from 3.81 mV and 1.59 mg/L to −8.05 mV and 1.08 mg/L, respectively. Among the microbial groups, the abundance of Virus, LNA, and Fungi decreased by 30.9%, 30.5%, and 34.9%, respectively, along the cascade system. EC, TOC, and NO₃⁻-N were identified as key drivers of changes in the abundance of the Virus, LNA, and Fungi groups. The concentrations of carbon and nitrogen nutrients significantly influenced the ecological assessment indices. Cascade hydropower stations had a significant impact on PAC, BAI, and VRC, while their influence on FMC was relatively small. The VRC showed a decreasing trend, suggesting a weakening effect of the stations on VRC. This study offers new perspectives and methods that facilitate the rapid and quantitative assessment of the ecological impacts of cascade hydropower stations. Full article

Feline mammary carcinoma (FMC) is an aggressive neoplasm with a poor prognosis. Clinical staging is crucial for risk assessment, yet the current WHO system lacks granularity, particularly in Stage III. Aligning this system with human breast cancer staging has the potential to improve prognostic accuracy. Additionally, prognostic factors such as tumor size, ulceration, lymph node metastasis, and lymphovascular invasion require further evaluation. This study retrospectively analyzed 75 female cats with FMC to assess the prognostic impact of clinicopathological factors and evaluate a novel staging system (new staging) adapted from the AJCC Cancer Staging Manual. Survival analyses included disease-free interval (DFI), and overall survival (OS). Tumor size >3 cm (p < 0.001), ulceration (p = 0.010), lymphovascular invasion (p < 0.001), lymph node metastasis (p < 0.001), WHO and new staging (p < 0.001) were significantly associated with shorter survival. The new staging refined Stage III into III_A (T₃N₀M₀), III_B (T₄N₀M₀), and III_C (AnyTN₁M₀), improved prognostic differentiation. Stage III_C cases had the worst survival (p < 0.001). Multivariate survival analysis identified lymphovascular invasion (HR = 2.834, 95% CI: 1.546–5.195, p = 0.001), histological Grade II (HR = 5.013, 95% CI: 1.122–22.397, p = 0.035) and III (HR = 9.894, 95% CI: 2.195–44.594, p = 0.003) and skin ulceration (HR = 2.462, 95% CI: 1.256–4.825, p = 0.009). These findings support the prognostic relevance of key clinicopathological factors in FMC and highlight the advantages of a refined TNM-based staging system, which may enhance risk stratification and therapeutic decision-making in veterinary oncology. Full article

Ultrasonic imaging methods show significant advantages in detecting internal defects of composite crystalline materials. For polymer-bonded explosives (PBXs) with highly filled crystalline particles, the strong acoustic attenuation caused by their heterogeneous crystalline structure leads to low signal-to-noise ratios (SNRs) in the full matrix capture (FMC) signals and strong background noise in reconstructed images. To realize the high-SNR imaging of defects in PBXs, this paper is the first to schematically reorganize the nonlinear post-process algorithms which have the potential to realize high-SNR imaging of defects in crystalline particle-filled explosives. Six kinds of beamforming algorithms (DAS, F-DMAS, BB-DMAS, DMAS3, L-DMAS, and DS-DMAS) were applied to the same FMC data to reconstruct the images of prefabricated side-drilled holes (SDHs) in PBXs. The image quality in terms of SNR, lateral and axial resolution, and calculation efficiency was compared and evaluated quantitatively. The experimental results show that the nonlinear beamforming algorithms showed significant improvements in SNR and resolution. In particular, L-DMAS and DS-DMAS exhibited excellent imaging capability in SDH defect detection compared to the other algorithms, with effective suppression of crystalline structural noise. Full article

The 15-min city (FMC) is a people-oriented urban development strategy that aims to provide a higher quality of life by manifesting the people’s right to the city. This study proposes an FMC index that measures how close a specific area is to achieving the 15-min accessibility to the six social functions (living, working, supplying, caring, learning, and enjoying). In the case of Pasig City, social function service areas were generated in terms of walkability and walking speeds per age group. Grid-based and population-based FMCI were calculated based on the established weights of points of interest and social functions, as well as the barangay population distribution per age group. The results show that 90% of the barangays achieved an FMCI of 0.5 or higher. This study presents an in-depth yet replicable approach using open-source data, considering facilities in each social function based on necessity of each age group, as well as utilizing pedestrian walkability as an impedance. Further, high population-based FMCI barangays cluster in the southern-central part of the city. The developed FMCI offers a compelling rationale for other HUCs to assess urban planning strategies, such as zoning strategies in the context of the weighted importance of amenities, walkability, and population distribution. Full article

Detecting multiple tumor markers is of great importance. It helps in early cancer detection, accurate diagnosis, and monitoring treatment. In this work, gold nanoparticles–toluidine blue–graphene oxide (AuNPs-TB–GO) and gold nanoparticles–carboxyl ferrocene–tungsten disulfide (AuNPs–FMC–WS₂) nanocomposites were prepared for labeling Carcinoembryonic antigen (CEA) antibody and Carbohydrate antigen 72–4 (CA72-4) antibody, respectively, and used as two kinds of probes with different electrochemical signals. With the excellent magnetic performance of biotin immune magnetic beads (IMBs), the biofunctional IMBs were firmly deposited on the magnetic glassy carbon electrode (MGCE) surface by applying a constant magnetic field, and then the CEA and CA72-4 antibody were immobilized on the IMBs by the avidin–biotin conjugation. The assay was based on the change in the detection peak current. Under the optimum experimental conditions, the linear range of detection of CEA is of the two-component immunosensor is from 0.01 to 120 ng/mL, with a low detection limit of 0.003 ng/mL, and the linear range of detection of CA72-4 is from 0.05 to 35 U/mL, with a detection limit of 0.016 U/mL. The results showed that the proposed immunosensor enabled simultaneous monitoring of CEA and CA72-4 and exhibited good reproducibility, excellent high selectivity, and sensitivity. In particular, the proposed multiplexed immunoassay approach does not require sophisticated fabrication and is well-suited for high-throughput biosensing and application to other areas. Full article

This study proposes an Additive Wavelet Transform (AWT)-based method to fuse Multispectral UAV (MS UAV, 5 cm resolution) and Sentinel-2 satellite imagery (10–20 m resolution), generating 5 cm resolution fused images with a focus on near-infrared and shortwave infrared bands to enhance the accuracy of mango canopy water content monitoring. The fused Sentinel-2 and MS UAV data were validated and calibrated using field-collected hyperspectral data to construct vegetation indices, which were then used with five machine learning (ML) models to estimate Fuel Moisture Content (FMC), Equivalent Water Thickness (EWT), and canopy water content (CWC). The results indicate that the addition of fused Sentinel-2 data significantly improved the estimation accuracy of all parameters compared to using MS UAV data alone, with the Genetic Algorithm Backpropagation Neural Network (GABP) model performing best (R² = 0.745, 0.859, and 0.702 for FMC, EWT, and CWC, respectively), achieving R² improvements of 0.066, 0.179, and 0.210. Slope, canopy coverage, and human activities were identified as key factors influencing the spatial variability of FMC, EWT, and CWC, with CWC being the most sensitive to environmental changes, providing a reliable representation of mango canopy water status. Full article

A creative approach to reducing food waste by reusing industrial cake waste can result in the development of a worldwide favorite snack. This study aimed to evaluate the potential of industrial cake wastes—basic mini cake (BMC), filled mini cake (FMC), and traditional mini cake (TMC)—as sustainable alternatives to wheat flour in producing ring-shaped cookies. After initial screening, FMC was upcycled at 10%, 15%, 20%, 30%, and 50% of wheat flour replacement for ring-shaped cookie processing. Only the formulations containing 10, 15, and 20% FMC waste showed good moldability and similar rheological parameters between each other (p < 0.05). After baking, the cookies made with FMC waste showed greater volume expansion (p < 0.05) compared to the standard formulation. All cookies had water activity below 0.6 and remained stable during the 150 days of storage. Furthermore, after 150 days of storage, the hardness of the standard (T1) formulation was 40.02 N, while formulations with FMC waste (10%—T2, 15%—T3, and 20%—T4) resulted in softer cookies, with hardness values of 26.9 N, 27.9 N, and 27.61 N, respectively. The ring-shaped cookies containing 15% FMC waste showed the best technological performance, considering manufacture, physicochemical, and hardness traits, with no nutritional differences compared to the control. Full article

In arid areas, water shortage has become a major bottleneck limiting the sustainable development of agriculture, necessitating improved water use efficiency and the full development of innovative water-saving irrigation management technologies to improve quality. In the present study, tomato (Solanum lycopersicum cv. Micro Tom) fruits were used as materials, and different irrigation frequencies were set during the fruit expansion stage. The normal treatment (CK) was irrigated every three days, while the water deficit treatments were irrigated at varying frequencies: once every 4 days (T1), 5 days (T2), 6 days (T3), 7 days (T4), and 8 days (T5). These corresponded to 80%, 70%, 60%, 50%, and 40% of the maximum field moisture capacity (FMC), respectively, with CK maintaining full irrigation at 90% of the maximum FMC. The water deficit treatment T3, with less stress damage to plants and the most significant effect on fruit quality improvement, was selected based on plant growth indices, photosynthetic characteristics, chlorophyll fluorescence parameters, and fruit quality indices, and its effects on carotenoids, glycolic acid fractions, and volatile compounds during tomato fruit ripening were further investigated. The outcome indicated that moderate water deficit significantly increased the carotenoid components of the tomato fruits, and their lycopene, lutein, α-carotene, and β-carotene contents increased by 11.85%, 12.28%, 20.87%, and 63.89%, respectively, compared with the control fruits at the ripening stage. The contents of glucose and fructose increased with the development and ripening of the tomato fruits, and reached their maximum at the ripening stage. Compared to the control treatment, the moderate water deficit treatment significantly increased the glucose and fructose levels during ripening by 86.70% and 19.83%, respectively. Compared to the control conditions, water deficit conditions reduced the sucrose content in the tomato fruits by 27.14%, 18.03%, and 18.42% at the mature green, turning, and ripening stages, respectively. The moderate water deficit treatment significantly increased the contents of tartaric acid, malic acid, shikimic acid, alpha ketoglutaric acid, succinic acid, and ascorbic acid, and decreased the contents of oxalic acid and citric acid compared to the control. The contents of total soluble sugar and total organic acid and the sugar–acid ratio were significantly increased by 48.69%, 3.71%, and 43.09%, respectively, compared with the control at the ripening stage. The moderate water deficit treatment increased the fruit response values to each sensor of the electronic nose, especially W5S, which was increased by 28.40% compared to the control at the ripening stage. In conclusion, during the ripening process of tomato fruit, its nutritional quality and flavor quality contents can be significantly improved under moderate (MD) deficit irrigation treatment. The results of this experiment can lay the foundation for the research on the mechanism of water deficit aiming to promote the quality of tomato fruit, and, at the same time, provide a theoretical basis and reference for tomato water conservation and high-quality cultivation. Full article