Search Results (32)

A four-component blend comprising pear ester, DMNT, linalool oxide, and acetic acid (CM4K) was identified as a potent allelochemical lure for both sexes of codling moth (CM), Cydia pomonella (L.). Studies conducted from 2020 to 2022 in Washington State (USA) examined factors which could impact the lure’s relative performance. The CM4K lure was effective across a range of mating disruption programs and was equally attractive in monitoring wild and sterile CM. The lure remained attractive for at least 10 weeks. Total catch in traps baited with the CM4K was significantly less impacted than a sex pheromone lure located near mating disruption dispensers and female catches were largely unaffected. Traps with the CM4K lure caught significantly more females and fewer males when placed near clusters of fruits in a trellised orchard. Two factors were found to significantly impact the relative performance of the CM4K to sex pheromone lures: the CM4K lure was only equivalent to sex pheromone lures in pear MD orchards, and apple and pear orchards with vigorous weed growth. This is the first report of a monitoring lure for a tortricid moth being negatively impacted by the background odor of non-host weed species present within an orchard. Full article

The use of CpGV strains as the basis for bioinsecticides is an effective and safe way to control Cydia pomonella. The research is aimed at the identification and study of new CpGV strains. Objects of identification and bioinformatic analysis: 18 CpGV strains. Sequencing was carried out on a NextSeq550. Genome assembly and annotation were carried out using Spades, Samtools 1.9, MinYS, Pilon, Gfinisher, Quast, and Prokka. Comparative genomic analysis was carried out in relation to the reference genome present in the «Madex Tween» strain-producer (biological standard) according to the average nucleotide identity (ANI) criterion. The presence/absence of IAP, cathepsin, MMP, and chitinase in the genetic sequences of the strains was determined using simply phylogeny. Entomopathogenic activity was assessed against C. pomonella according to the criterion of biological efficacy. Thus, molecular genetic identification revealed that 18 CpGV strains belong to a genus of Betabaculovirus. For all the strains under study ANI values of 99% or more were obtained, and the presence of the cathepsin, chitinase, IAP, and MMP genes was noted. The strains BZR GV 1, BZR GV 3, BZR GV 7, BZR GV 10, and BZR GV L-8 showed the maximum biological efficacy: 100% on the 15th day of observation. Strains BZR GV 4, BZR GV 8, and BZR GV 13 showed efficacy at the level of the «Madex Tween» preparation: 89.5% on the 15th day of observation. The strains with the highest mortality rate of the host insect were identified: BZR GV 9, BZR GV 10, BZR GV L-6, and BZR GV L-8. Full article

Cydia pomonella granulovirus is a natural pathogen for Cydia pomonella that is used as a biocontrol agent of insect populations. The study of granulovirus virulence is of particular interest since the development of resistance in natural populations of C. pomonella has been observed during the long-term use of the Mexican isolate CpGV. In our study, we present the genomes of 18 CpGV strains endemic to southern Russia and from Kazakhstan, as well as a strain included in the commercial preparation “Madex Twin”, which were sequenced and analyzed. We performed comparative genomic analysis using several tools. From comparisons at the level of genes and protein products that are involved in the infection process of virosis, synonymous and missense substitution variants have been identified. The average nucleotide identity has demonstrated a high similarity with other granulovirus genomes of different geographic origins. Whole-genome alignment of the 18 genomes relative to the reference revealed regions of low similarity. Analysis of gene repertoire variation has shown that BZR GV 4, BZR GV 6, and BZR GV L-7 strains have been the closest in gene content to the commercial “Madex Twin” strain. We have confirmed two deletions using read depth coverage data in regions lacking genes shown by homology analysis for granuloviruses BZR GV L-4 and BZR GV L-6; however, they are not related to the known genes causing viral pathogenicity. Thus, we have isolated novel CpGV strains and analyzed their potential as strains producing highly effective bioinsecticides against C. pomonella. Full article

In insects, the chemical senses influence most vital behaviors, including mate seeking and egg laying; these sensory modalities are predominantly governed by odorant receptors (ORs), ionotropic receptors (IRs), and gustatory receptors (GRs). The codling moth, Cydia pomonella, is a global pest of apple, pear, and walnut, and semiochemically based management strategies limit the economic impacts of this species. The previous report of expression of a candidate pheromone-responsive OR in female codling moth ovipositor and pheromone glands raises further questions about the chemosensory capacity of these organs. With an RNA-sequencing approach, we examined chemoreceptors’ expression in the female codling moth abdomen tip, sampling tissues from mated and unmated females and pupae. We report 37 ORs, 22 GRs, and 18 IRs expressed in our transcriptome showing overlap with receptors expressed in adult antennae as well as non-antennal candidate receptors. A quantitative PCR approach was also taken to assess the effect of mating on OR expression in adult female moths, revealing a few genes to be upregulated or downregulating after mating. These results provide a better understanding of the chemosensory role of codling moth female abdomen tip organs in female-specific behaviors. Future research will determine the function of specific receptors to augment current semiochemical-based strategies for codling moth management. Full article

In eastern North America, apple orchards are often attacked by several species of tortricid moths (Lepidoptera), including Cydia pomonella, Grapholita molesta, Argyrotaenia velutinana, and Pandemis limitata. Sex pheromones are routinely used to monitor male moth populations. Adding plant volatiles to monitoring traps could increase the capture of moths of both sexes and improve the effectiveness of mating disruption systems. This study sought to quantify the attraction of adults of four tortricid moth species to five olfactory treatments, namely (1) Pherocon^® CM L2-P, (2) Pherocon Megalure CM 4K Dual^® (=Megalure), (3) Megalure + benzaldehyde, (4) TRE 2266 (linalool oxide + (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT)), and (5) TRE 2267 (linalool oxide + DMNT + benzaldehyde), in non-mating disrupted commercial apple orchards in Massachusetts. The commercial lure Megalure was attractive to both sexes of G. molesta and C. pomonella. The addition of benzaldehyde to TRE 2266 or to Megalure significantly increased the capture of male G. molesta during the mid and late season of 2021. Only when benzaldehyde was added to TRE 2266 did the latter lure attract P. limitata in 2020 and 2021. The greatest number of tortricid moths (all four species combined) was captured by TRE 2267. This finding highlights the opportunity to enhance the attractiveness of a commercial lure through the addition of benzaldehyde, an aromatic compound, to Megalure. The potential of these additional volatiles to detect moths in a mating-disrupted orchard and/or remove female moths as a component of a management system is discussed. Full article

Serine protease inhibitors (serpins) appear to be ubiquitous in almost all living organisms, with a conserved structure and varying functions. Serpins can modulate immune responses by negatively regulating serine protease activities strictly and precisely. The codling moth, Cydia pomonella (L.), a major invasive pest in China, can cause serious economic losses. However, knowledge of serpin genes in this insect remain largely unknown. In this study, we performed a systematic analysis of the serpin genes in C. pomonella, obtaining 26 serpins from the C. pomonella genome. Subsequently, their sequence features, evolutionary relationship, and expression pattern were characterized. Comparative analysis revealed the evolution of a number of serpin genes in Lepidoptera. Importantly, the evolutionary relationship and putative roles of serpin genes in C. pomonella were revealed. Additionally, selective pressure analysis found amino acid sites with strong evidence of positive selection. Interestingly, the serpin1 gene possessed at least six splicing isoforms with distinct reactive-center loops, and these isoforms were experimentally validated. Furthermore, we observed a subclade expansion of serpins, and these genes showed high expression in multiple tissues, suggesting their important roles in C. pomonella. Overall, this study will enrich our knowledge of the immunity of C. pomonella and help to elucidate the role of serpins in the immune response. Full article

Codling moth, Cydia pomonella L., is a major pest of pome fruits and walnuts worldwide. Screening the susceptibility of apple varieties to C. pomonella infestation is an important step to develop a sustainable management program against this pest. Therefore, the present study aimed to explore potential correlations between pest damage and several physical (firmness, weight, and diameter), chemical (pH, SSC, and TA), and biochemical (polyphenols) fruit properties collected from two sites with distinctively twelve and seven commercial apple varieties. The study was conducted in two distinct Moroccan regions to highlight whether the traits influencing the insect’s preference could be similar for different varieties under contrasted climates. In both regions, results revealed that the pest damage varied significantly across apple varieties as well as between observation dates. The late and medium maturing varieties had similar damage patterns and were heavily attacked compared to early maturing ones. The preferred varieties were Galaxy Gala, Obro Gala, Golden Smoothee, Black Staymen, and Idared, while the least preferred ones were Anna, Dorsett, and Stark Delicious. Some physical and chemical fruit properties seemed to be associated with the insect behavior, in particular, firmness and pH were positively correlated to pest damage. Moreover, the research suggested that infestations might be influenced by diameter, weight, and polyphenol content of fruits. Full article

The codling moth, Cydia pomonella (L.), is an invasive agricultural pest of pome fruits and walnuts in China that threatens the apple industry in the Loess Plateau and Bohai Bay; it has developed resistance to many insecticides. Sterile insect technique (SIT) combined with area-wide integrated pest management (AW-IPM) can reduce the risk of resistance to insecticides and effectively control some insect pest species. Our previous laboratory experiment found that irradiation with 366 Gy of X-ray caused the males of the codling moth to become sterile. However, the sterility and adaptability of males after being irradiated with 366 Gy X-ray in the field are still unclear. In this study, we investigated the effect of X-ray irradiation on the fitness of male adults that emerged from pupae irradiated with 366 Gy to explore their adaptability and mating competitiveness, and to examine the effect of releasing sterile male insects in orchards in northeast China on the fruit infestation rate of the Nanguo pear. The results showed that 366 Gy of X-ray irradiation significantly reduced the mating competitiveness of males and the hatching rate of the eggs laid by females pairing with sterile males. Meanwhile, the lifespan of the sterile male moths was significantly shorter than that of the normal ones in the field. A pilot test showed that the release twice of sterile male moths in the orchards had no significant effect on the fruit infestation rate. Our field experiments provide a scientific basis for the further optimization of the SIT technology program for controlling C. pomonella. Full article

Deep neural networks (DNNs) have recently been applied in many areas of agriculture, including pest monitoring. The codling moth is the most damaging apple pest, and the currently available methods for its monitoring are outdated and time-consuming. Therefore, the aim of this study was to develop an automatic monitoring system for codling moth based on DNNs. The system consists of a smart trap and an analytical model. The smart trap enables data processing on-site and does not send the whole image to the user but only the detection results. Therefore, it does not consume much energy and is suitable for rural areas. For model development, a dataset of 430 sticky pad photos of codling moth was collected in three apple orchards. The photos were labelled, resulting in 8142 annotations of codling moths, 5458 of other insects, and 8177 of other objects. The results were statistically evaluated using the confusion matrix, and the developed model showed an accuracy > of 99% in detecting codling moths. This developed system contributes to automatic pest monitoring and sustainable apple production. Full article

The systematic and long-term use of pesticides in fruit plantations leads to the formation of resistant pest populations. The purpose of this work was to evaluate the effectiveness of the use of entomophages and acariphages for the protection of apple orchards. Against the dominant pest Cydia pomonella (Linnaeus), Habrobracon hebetor (Say) was used, which was caught in the Krasnodar Territory using cassettes with caterpillars attractive to H. hebetor. To determine the most genetically high-quality population, an RAPD analysis was carried out from three Russian (Krasnodar, Stavropol, and Belgorod) and one Kazakh (Shymkent) populations of H. hebetor, which revealed a high level of DNA polymorphism and genetic diversity in the studied geographical populations of the cities of Krasnodar and Stavropol. The efficiency of the captured Krasnodar population of H. hebetor against C. pomonella was about 75%. To regulate the number of aphids Aphis pomi De Geer and Tetraneura caerulescens (Pass.), breeding reserves of the aphidophages Harmonia axyridis Pallas, Leis dimidiata Fabr., Cycloneda sangvinea L., and Aphidius colemani Vier. were established. The biological efficiency of the developed technique was 82.8–88.6%. The release of the acariphages Amblyseius andersoni (Chant) and Metaseiulus occidentalis (Nesb.) on the apple tree showed effectiveness from 80 to 90% against Tetranychus urticae Koch and Panonychus ulmi (Koch). To study the possibility of simultaneous use of entomophages and insecticides, experiments were carried out to study the sensitivity of H. hebetor and H. axiridis to insecticides. When H. hebetor cocoons were treated with Insegar^® and Atabron^®, the ectoparasitoid emergence values were 98.4% and 100%, respectively. The survival of adult H. axiridis treated with Madex twin^®, Atabron^®, and Koragen^® on the fifth day was 97.3%, 89.6%, and 81.9%, respectively. Based on the data obtained, it can be argued that it is possible to create favorable conditions for entomophages, which effectively regulate pest numbers in apple orchards. Full article

The codling moth, Cydia pomonella L. (Tortricidae), is a major pest of apples, potentially causing annual losses exceeding USD62 billion globally. Growers have limited options for combatting the codling moth. Sprays with azinphos-methyl have been banned in the European Union, Turkey, and the USA. To be effective, Bacillus thuringiensis or Carpocapsa pomonella must be ingested in large quantities, and the fruit is damaged before the larvae die. Mating disruption or an attract-and-kill strategy does not resolve problems caused by the migration of moths from adjacent areas or insecticide resistance. Discouraging neonates from burrowing into the fruit with feeding deterrents or repellents of plant origin may become a new strategy. This paper presents a list of twenty-three plants and six secondary metabolites preventing apple infestation by codling moth neonates. Some of these plant extracts or oils (Alium sativum, Tanacetum vulgare, Atremisia arborescens, Ginkgo biloba) showed deterrence exceeding 95% in comparison to controls. The prospects of codling moth control with botanicals are discussed, and further studies on these substances are suggested. In conclusion, the author states that twenty-nine plant-originated materials have great potential in organic apple protection. Future studies should concentrate on formulating these botanicals and identifying their molecular targets. Full article

The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is one of the major pests in pome fruit production worldwide. Heavy treatment of the larvae of C. pomonella with insecticides triggered the development of resistance to many groups of insecticides. In addition, the increasing concern about the adverse effects of synthetic insecticides on human health and the environment has led to the development of sustainable and eco-friendly control practices for C. pomonella. The entomopathogenic nematodes (EPNs) (Steinernema and Heterorhabditis spp.) and their endosymbionts (Xenorhabdus and Photorhabdus spp.) represent a newly emerging approach to controlling a wide range of insect pests. In the present study, field surveys were conducted in apple orchards to isolate and identify EPNs and their endosymbionts and evaluate their insecticidal efficacy on the larvae of C. pomonella. EPNs were isolated from 12 of 100 soil samples (12%). Seven samples were identified as Steinernema feltiae (Filipjev, 1934) (Rhabditida: Steinernematidae), whereas five samples were assigned to Heterorhabditis bacteriophora (Poinar, 1976) (Rhabditida: Heterorhabditidae). The pathogenicity of the EPN species/isolates was screened on the last instar larvae of G. mellonella. The two most pathogenic isolates from each EPN species were tested against fifth instar larvae of C. pomonella under controlled conditions. The maximum mortality (100%) was achieved by all EPN species/isolates at a concentration of 100 IJs/larva 96 h after treatment. The endosymbionts of selected H. bacteriophora and S. feltiae species were identified as Photorhabdus luminescens subsp. kayaii and Xenorhabdus bovienii, respectively. The mortality rates ranged between 25 and 62% when the fifth larval instar larvae of C. pomonella were exposed to the treatment of cell-free supernatants of symbiotic bacteria. In essence, the present survey indicated that EPNs and their symbiotic bacteria have good potential for biological control of C. pomonella. Full article

Apple is one of the most important economic fruit crops in the world. Despite all the strategies of integrated pest management (IPM), insecticides are still frequently used in its cultivation. In addition, pest phenology is extremely influenced by changing climatic conditions. The frequent spread of invasive species, unexpected pest outbreaks, and the development of additional generations are some of the problems posed by climate change. The adopted strategies of IPM therefore need to be changed as do the current monitoring techniques, which are increasingly unreliable and outdated. The need for more sophisticated, accurate, and efficient monitoring techniques is leading to increasing development of automated pest monitoring systems. In this paper, we summarize the automatic methods (image analysis systems, smart traps, sensors, decision support systems, etc.) used to monitor the major pest in apple production (Cydia pomonella L.) and other important apple pests (Leucoptera maifoliella Costa, Grapholita molesta Busck, Halyomorpha halys Stål, and fruit flies—Tephritidae and Drosophilidae) to improve sustainable pest management under frequently changing climatic conditions. Full article

Codling moth (Cydia pomonella L.) is a very important pest in apple, pear, and walnut orchards worldwide, including the USA. Cydia pomonella granulovirus (CpGV) is used to control codling moth in organic and conventional production. Due to increasing codling moth infestations from organic apple orchards in Washington State, USA, five codling moth colonies (WA1-WA5) were screened for their susceptibility relative to the isolate GV-0001, the main active ingredient of Cyd-X^®, using a discriminating concentration of 6 × 10⁴ OB/mL. Compared to a susceptible laboratory colony, the observed results indicated that GV-0001 lacked efficacy against codling moth colony WA3. It was confirmed that WA3 was the first case of codling moth resistance to CpGV in the USA. Further testing of WA3 was performed on a range of CpGV isolates and a lack of efficacy was observed against additional isolates. However, three newly developed CpGV preparations can efficiently infect larvae from the resistant colony WA3. Our results suggest that there is an urgent need to monitor the situation in the USA, aiming to prevent the emergence or spread of additional codling moth populations with CpGV resistance. Strategies to sustain the efficacy of codling moth control using novel CpGV formulations need to be developed. Full article

Codling moth (CM), Cydia pomonella L., is an important pest of apples worldwide. CM resistance to insecticides is a serious problem in apple production. For effective management and control, monitoring of resistant CM populations is absolutely necessary. Therefore, in this study, we investigated whether it is possible to find a reliable pattern of differences in CM populations related to the type of apple control method. The genetic results showed low estimated value of the pairwise fixation index, F_ST = 0.021, which indicates a lack of genetic differentiation and structuring between the genotyped populations. Different approaches were used to analyze the genetic structure of codling moth populations: Bayesian-based model of population structure (STRUCTURE), principal component analysis (PCA), and discriminant analysis of principal components (DAPC). STRUCTURE grouped the CM genotypes into two distinct clusters, and the results of PCA were consistent with this. The DAPC revealed three distinct groups. However, the results showed that population genetic differentiation between organic and integrated orchards was not significant. To confirm the genetic results, the forewing morphology of the same CM individuals was examined using geometric morphometric techniques based on the venation patterns of 18 landmarks. The geometric results showed higher sensitivity and separated three distinct groups. Geometric morphometrics was shown to be a more sensitive method to detect variability in genotypes due to pest control management. This study shows the possibility of using a novel method for a strategic integrated pest management (IPM) program for CM that is lacking in Europe. Full article