Search Results (22,412)

Essential oils are multicomponent natural products with potential neurobehavioral activity, but integrated comparative studies remain limited. This study compared the essential oils of Satureja brevicalyx, Peperomia dolabriformis, and Rosmarinus officinalis in relation to their chemical profiles, predicted target interactions, preliminary acute oral safety, anxiolytic-like and antidepressant-like effects, antagonist-sensitive behavioral patterns, and exploratory serum biomarkers. Oils were characterized by GC-MS, and their constituents were screened by molecular docking against anxiety-, depression-, sleep-, and stress-related targets. Independent cohorts of male BALB/c mice received oral essential oils (25–100 mg/kg) and were assessed in anxiety-related, depression-related, and locomotor behavioral paradigms, including the elevated plus maze, light–dark box, marble burying, tail suspension, forced swim, and open field tests. Flumazenil and WAY-100635 were used to examine whether the behavioral responses were sensitive to γ-aminobutyric acid type A (GABA-A)/benzodiazepine- and serotonin 1A (5-HT1A)-related pharmacological modulation, respectively. In a preliminary 24-h acute oral toxicity screen, no mortality was observed up to 5000 mg/kg. The three oils produced anxiolytic-like and antidepressant-like effects without reducing spontaneous locomotor activity. Within its experimental block, S. brevicalyx showed the most consistent flumazenil-sensitive anxiolytic-like pattern and FDR-significant reductions in corticosterone and TNF-α, together with increased IL-4. P. dolabriformis showed a broader predicted multitarget docking profile and antagonist-sensitive behavioral attenuation compatible with mixed pathway participation. R. officinalis produced significant but more moderate behavioral effects. WAY-100635 partially attenuated the antidepressant-like effects of all three oils. These findings support differentiated but convergent functional neurobehavioral profiles among the oils. The docking, antagonist, and biomarker results should be interpreted as hypothesis-generating evidence of possible pathway involvement, supporting further validation in chronic stress models, receptor-specific assays, pharmacokinetic studies, and expanded safety evaluations. Full article

Wastewater containing heavy metals and dyes poses serious environmental risks. This study developed a multifunctional composite by coating polyurethane foam (PUF) with milled activated carbon (mAC) and a zirconium-based metal–organic framework (Zr-MOF) for the simultaneous removal of hexavalent chromium (Cr(VI)) and Congo red (CR). The composite was synthesized using a hydrothermal method to grow Zr-MOF on the surface. The SEM analysis confirmed the successful incorporation of mAC and surface modification with Zr-MOF, which resulted in increased surface roughness and porous morphology. XRD and FTIR confirmed the presence of organic ligands connected to the metal structure and the functional groups of each component in composite materials. The optimum conditions for Zr-MOF/mAC/PUF adsorption (nearly 100% removal) in the binary Cr(VI)/CR solution (50 mg/L each) were 25 °C, pH 9, and 150 rpm for 24 h. The Zr-MOF/mAC/PUF was hydrophilic with a swelling ratio of 2.64 g/g. The thermodynamic investigation of Zr-MOF/mAC/PUF resulted in 141.6218 kJ/mol for Cr(VI) and 166.111 kJ/mol for CR of ΔH° (rapid adsorption), negative ΔG° (spontaneous adsorption), a high positive value of ΔS° (disorder structure) and low activation energy (approximately 2.5 to 2.8 kJ/mol). After analyzing the isotherm and reaction kinetics, the possible mechanism could be endothermic physicochemical adsorption and pseudo-second-order kinetic behavior, with electrostatic attraction and diffusion control. The study of 6-times-reused Zr-MOF/mAC/PUF adsorption identified as a decrease of 7.55 percentage point without changing notable morphology and functional groups, based on SEM and FTIR. Full article

The longissimus dorsi (LD) and biceps femoris (BF) muscles are important meat-producing regions in camels. Investigating differences in meat quality and proteomic profiles between the LD and BF muscles in Junggar Bactrian camels can provide a molecular basis for regulating camel meat quality and genetic improvement. In this study, 20 healthy adult male Junggar Bactrian camels were selected. Following slaughter, muscle samples were collected from the splenius (SP), triceps brachii (TB), LD, external oblique (EO), gluteus medius (GM), and BF. Meat quality parameters (pH, meat color, shear force, drip loss, and cooking loss) were measured. The LD exhibited the highest meat quality among the six cuts, in contrast to the BF, which showed the lowest. Proteomic analysis of LD and BF from 6 Junggar Bactrian camels was conducted to identify proteins associated with meat quality, yielding 81 differentially expressed proteins (DEPs). Gene Ontology (GO) enrichment analysis highlighted several significantly enriched terms among the DEPs (p < 0.05), including calcium-dependent phospholipid binding, zinc ion binding, and metal ion binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis (p < 0.05) further indicated notable enrichment in cytoskeletal organization, 2-oxocarboxylate metabolism, and the citric acid cycle. DEPs associated with meat quality were identified, including tubulin α-chain-like 3 and synaptic function regulator FMR1 isoform X15, which can serve as candidate DEPs for shear force. Protein phosphatase 1 regulatory subunit 14C isoform X1 can serve as a candidate differentially expressed protein for pH. Protein phosphatase 1 regulatory subunit 14C isoform X and anchoring protein repeat domain 1 can serve as candidate DEPs for cooking loss. Membrane-associated protein A4 and membrane-associated protein A7 isoform X1, as well as the transcriptional activator of cytochrome c oxidase 1, can serve as candidate DEPs for color a*. These data may serve as a reference for further studies on how different cuts affect meat quality and for practical efforts to improve camel meat quality. Full article

This study investigates the influence of cold-working deformation on the electromotive force (EMF) calibration characteristics, hysteresis behavior, and long-term stability of the Type-K mineral-insulated metal-sheathed (MIMS) thermocouples used in Combination Fixed In-Core Detector Assemblies for pressurized water reactor nuclear power plants. Reduction ratios of 12%, 28%, and 38% were investigated, and samples were subjected to heating–cooling calibration and in situ aging tests. The results show that increased cold-working deformation leads to greater negative EMF deviation and larger heating–cooling hysteresis, mainly affected by the degradation of the positive KP thermoelement. Cold-working lowers the atomic diffusion activation energy and accelerates element migration, resulting in pronounced EMF drift during isothermal aging at 350 °C for 720 h. After aging below the order–disorder transition temperature, stable ordered structures form in the thermoelement alloys and hysteresis is significantly reduced. However, within the range investigated in this study, deformation above 28% imparts irreversible effects. The EMFs of 28% and 38% deformed samples remained lower than that of the undeformed state even after isothermal aging at 700 °C for 500 h. These findings reveal that excessive cold-working deformation severely impairs the measurement accuracy and long-term stability of the thermocouples, highlighting the necessity of the strict control of drawing deformation to ensure the reliability of nuclear-grade thermocouples under both normal and abnormal reactor operating conditions. Full article

Cyclic adenosine monophosphate (cAMP) is a ubiquitous second messenger downstream of G protein-coupled receptors (GPCRs) and a central regulator of cellular signaling. Genetically encoded exchange proteins directly activated by cAMP (Epac)-based Förster resonance energy transfer (FRET) biosensors enable real-time monitoring of cAMP dynamics in living cells, but commonly used cyan/yellow FRET pairs require short-wavelength excitation, limiting compatibility with multiplex imaging and blue-light optogenetic tools such as bacterial photoactivated adenylyl cyclases (bPACs). Here, we engineered and systematically characterized four red-shifted Epac-based single-chain FRET cAMP sensors combining yellow or orange FRET donors with red fluorescent FRET acceptors. Using ratiometric live-cell imaging, we quantified stimulus-evoked FRET responses and identified Epac_red4 as the best-performing variant, showing an approximately 55% decrease in normalized FRET after forskolin stimulation. Epac_red4 also reliably detected G_i/o-mediated decreases in cAMP following μ-opioid receptor activation. Brief 405 nm light pulses induced graded and reversible cAMP elevations using the low dark-activity variant bPAC-F198Y. Furthermore, Epac_red4 enabled analysis of cAMP recovery kinetics during phosphodiesterase inhibition and multiplex imaging of cAMP and intracellular Ca²⁺ using Fura-2 with minimal spectral and pH-related interference under physiological imaging conditions. Together, Epac_red4 represents a robust red-shifted cAMP sensor for optogenetic and multiplex signaling studies. Full article

A fly ash-based artificial aggregate with a compressive strength of >60 MPa was prepared via cement activation and alkali activation using >75% fly ash as the principal raw material. The mechanical properties of concrete prepared using this aggregate and the characteristics of the interfacial transition zone (ITZ) were compared with those of concrete containing natural aggregate. The results indicated that the compressive strength of concrete prepared using artificial aggregate was lower than that of concrete prepared using natural aggregate by about 19.0–27.6%. Scanning electron microscopy (SEM) revealed that the cement paste bonded tightly to the surface of the natural aggregate; the width of ITZ was 20–30 µm. The ITZ between the cement paste and the fly ash-based artificial aggregate exhibited a relatively loose structure at 28 d, with a width of 30–40 µm; however, the ITZ became narrower and denser at 90 d. EDS indicated that the principal hydration products were calcite crystals and C-S-H gel in the ITZ of natural aggregate concrete and artificial aggregate concrete. According to nanoindentation tests, for both cement pastes with natural and artificial aggregates, the elastic modulus of the ITZ at 28 d was >10 GPa, and it increased slightly at 90 d. The ITZ between the alkali-activated paste and limestone exhibited a relatively dense structure, with a width of 20–30 µm. The ITZ between the alkali-activated paste and the fly ash-based artificial aggregate exhibited a relatively loose structure with numerous pores at 28 d and had a width of 30–40 µm; however, the ITZ became narrower and denser at 90 d. The principal hydration products were N-A-S-H and C-A-S-H in the two kinds of aggregate concrete. Whether the alkali-activated paste contained natural aggregate or artificial aggregate, the elastic modulus of the ITZ at 28 d was 5–6 GPa, and it increased rapidly to >10 GPa by 90 d. The performance of ITZ is primarily influenced by the matrix materials, while also being influenced by aggregates and curing conditions. Qualitative and quantitative analyses revealed the formation mechanisms of artificial and natural aggregates in different matrices. Through continuous hydration and polymerization reactions, artificial aggregates gradually form narrower and denser interfacial transition zones with different matrices, especially in alkali-activated matrices. The continuously improved performance of the ITZ makes it less prone to forming cracks between the ITZ and the artificial aggregate. This study provides an important theoretical basis for the application of fly ash-based artificial aggregates, which can also be used to produce high-strength concrete. Full article

Nanoparticles of the aminopolysaccharide chitosan (ChNPs) are effective delivery platforms for biologically active substances for agrobiotechnological applications and hold great promise for solving precision problems in sustainable and efficient agriculture. This review presents an analysis of research publications during the past 20 years examining methods for producing ChNPs through ionotropic gelation using sodium tripolyphosphate for cross-linking macrochains, which are of practical interest for agriculture. Key aspects of the nanostructure formation process are analyzed, including the influence of the physicochemical characteristics of the aminopolysaccharide, the concentration and ratio of reagents, and ionic cross-linking conditions on the average size, size distribution (polydispersity), and zeta potential of nanoparticles. Particular attention is paid to several approaches proposed in the literature for determining optimal gelation conditions to obtain ChNPs with pre-specified size characteristics. Potential applications of nanostructured preparations based on these nanoparticles for agrobiochemical purposes are considered, including the encapsulation of antifungal, antiviral and antimicrobial agents, pesticides, NPK fertilizers, metal ions, plant extracts, essential oils, etc., to develop biodegradable stimulants for seed germination and plant growth, increased crop yields, and improved agricultural product quality. It is concluded that blocking the protonated amino groups of chitosan with tripolyphosphate anions is undesirable due to the reduced biological activity of the macromolecules and the nanostructured preparations obtained therefrom. An alternative approach for producing ChNPs with high biological activity with neither use of cross-linking agents nor encapsulation of agrochemicals is described. Full article

Soybean residue, a major by-product of soy milk and tofu processing, is a large-yield and nutrient-rich substrate, but it is currently experiencing low utilization rates. In this study, a prodigiosin-producing strain, Serratia marcescens BD2025, was isolated from naturally fermented soybean residue. Subsequently, prodigiosin was produced by solid-state fermentation of soybean residue, and the fermentation conditions were further optimized by a Box–Behnken (BBD) model involving the soybean residue-to-water ratio, inoculum size, and temperature. Under the optimized conditions of a soybean residue-to-water ratio of 1:4.28, an inoculum size of 5.2%, and cultivation at 27.5 °C for 48 h, the prodigiosin yield reached 19.05 mg/g. High-performance liquid chromatography analysis showed that the major pigment peak had a retention time comparable to that of a prodigiosin standard, with an estimated purity of 96% based on peak area normalization. The results show that prodigiosin remains relatively stable at below 40 °C, in acidic conditions, and in darkness. The extracted prodigiosin inhibited both Escherichia coli and Staphylococcus aureus, with stronger activity against S. aureus. The scavenging ability of DPPH radical was dose-dependent, with the scavenging activity of 93.13% at 5 mg/mL. These findings indicate that soybean residue can serve as a solid-state fermentation substrate for prodigiosin production and provide a basis for the value-added utilization of soybean-processing residues. Full article

Plant extracts in vegetable oils are foundational and eco-responsible for skin care, combining their emollient properties with other additional benefits, derived from their antioxidant, antimicrobial and UV-absorbing activity. The present research conducted a complex investigation of Usnea barbata extract in Karanja oil (KO), aiming for its further incorporation into various cosmetic formulations. The lichen extract (UBKO) was obtained through cold maceration. Phytochemical screening was performed using the Folin–Ciocalteu method and Graphite Furnace Atomic Absorption Spectrophotometry (GFAAS). Physicochemical properties were evaluated via Fourier Transform Infrared Spectroscopy (FTIR) and Atomic Force Microscopy (AFM). The rheological behavior and oxidative stability of the oil samples, UBKO and KO, were also investigated. UBKO had a slightly lower density (0.827 vs. 0.955) and pH (4.22 vs. 4.86) than KO, and a slightly higher oxidative resistance, quantified as the induction period (IP) value (6.45 vs. 6.00). The total phenolic-equivalent content (TPC, µg GAE/mL oil sample) was significantly greater in UBKO than in KO (567.16 ± 14.96 vs. 433.26 ± 22.96, p = 0.001). The values of minimum inhibitory concentration (MIC, mg/mL) indicated significantly higher antibacterial effect against S. aureus and antifungal effect against C. albicans for UBKO than KO (9.62 ± 2.87 vs. 31.25 ± 18.75, p = 0.049, and, respectively, 5.06 ± 1.68 vs. 37.50 ± 12.50, p = 0.01). Finally, our results showed that UBKO had an estimated sun-protective factor (SPF) of 30.9, slightly higher than 29.8 for the base oil formulation, KO; these findings represent baseline in vitro UV-absorbing trends. All of these results suggest that U. barbata extract in Karanja oil may exhibit complementary bioactive properties with potential applications in skincare. Full article

The North China Craton hosts abundant skarn iron resources, yet the regional large-scale mineralization mechanism remains incompletely understood. The Litun deposit is a newly discovered skarn iron deposit in the North China Craton. Integrated field geological investigations, petrographic observations, fluid inclusion microthermometry and stable isotope geochemistry are applied to constrain evaporite contributions to metallogenic processes. Four mineralization stages are identified: skarn, oxide, sulfide, and carbonate. Early skarn-stage fluids are iron-rich magmatic hydrothermal fluids with high temperatures (498 to >550 °C), high salinities (18.6 to 59.4 wt% NaCl eqv.), and magmatic δ¹⁸O values of 8.3 to 10.8‰. Subsequent oxide to late carbonate stages record continuous infiltration of meteoric water, supported by H–O isotopic trends of rising meteoric water proportions. Pyrite from the magnetite ores has δ³⁴S_V-CDT values between 12.0 and 15.0‰, significantly higher than those of pyrite in the Litun diorite (−0.8 to 1.1‰), indicating the contributions of sulfur from evaporites (δ³⁴S_V-CDT 26.9 to 28.6‰) in the mineralization process. Moreover, vein pyrite formed in later stages displays even higher δ³⁴S values (17.3 to 20.9‰), demonstrating progressive enrichment of evaporite-derived sulfur as hydrothermal activity evolves. Synchronous rises in meteoric water fraction and evaporite sulfur proportion indicate evaporites are delivered into the ore-forming system via meteoric water mixing. The mixing of meteoric water containing dissolved evaporites and iron-rich magmatic-hydrothermal fluids may be the major mechanism of magnetite precipitation in the Litun deposit. Full article

The development of efficient, low-cost hydrodeoxygenation (HDO) catalysts is essential for converting renewable lipids into sustainable aviation fuels. Here, we report a series of sulfided bimetallic NiFe/γ-Al₂O₃ catalysts and systematically investigate the promotional role of Fe in the HDO of methyl decanoate, a model lipid compound. Using complementary characterization together with fixed-bed reactor kinetic measurements, we elucidate the influence of the Ni/Fe ratio on catalyst structure, sulfidation behavior, electronic properties, and reaction pathway. Fe incorporation promotes Ni sulfidation and induces electron transfer from Fe to Ni, as directly evidenced by a red shift in the CO stretching frequency (from 2094 cm⁻¹ for Ni-only to 2090 cm⁻¹ for NiFe), indicating increased electron density on Ni sites and enhanced π-backdonation. Among the catalysts tested, N5F5 (Ni/Fe mass ratio = 1:1) exhibits the highest Ni sulfidation degree, the highest turnover frequency (32.1 h⁻¹), and the lowest apparent activation energy (E_a ≈ 92 kJ/mol). At 360 °C, it achieves 52.9% methyl decanoate conversion, far exceeding that of monometallic Ni and Fe catalysts. Product selectivity analysis reveals that sulfided Ni sites predominantly promote the decarboxylation/decarbonylation (DCOx) pathway, whereas Fe sites contribute only marginally to direct deoxygenation (DDO). This work provides the first direct spectroscopic evidence for Fe-to-Ni electron transfer in sulfided NiFe catalysts and establishes a clear structure-performance correlation, offering a rational design strategy for low-cost, high-performance HDO catalysts for lipid upgrading. Full article

To support the achievement of the carbon peaking and carbon neutrality goals and promote the resource utilization of industrial solid waste, a ternary solid waste composite cementitious material was prepared by blending ground granulated blast-furnace slag (GGBFS), fly ash (FA), and carbide slag (CS) with cement. The optimal mix ratio was determined through single-factor experiments and response surface methodology. The synergistic hydration mechanism was elucidated using microstructural characterization techniques, including XRD, FTIR, TG-DTG, and SEM. The composite material was then applied to a semirigid base course, and its mechanical properties and durability were systematically evaluated. The results indicate that the optimal levels of FA, GGBFS, and CS investigated in the single-factor experiments are 20–40%, 30–50%, and 2–6%, respectively. The optimal mix ratio of the ternary solid waste composite is 21.0% FA, 36.3% GGBFS, and 5.7% CS. The underlying microstructural mechanism is that carbide slag creates a highly alkaline environment, which activates the pozzolanic activity of GGBFS and fly ash, leading to the formation of hydration products dominated by C-(A)-S-H gel. With increasing curing age, the gel structure evolves from a loose and disordered state to a dense and ordered state, ultimately forming a compact microstructure based on a highly polymerized C-(A)-S-H gel matrix. The 7-day unconfined compressive strength of the stabilized gravel using the solid waste-based composite cementitious material reached 5.93 MPa, and the 28-day drying shrinkage coefficient was reduced by 18.3% compared with that of cement-stabilized gravel. After 18 freeze–thaw cycles, the compressive strength increased by 2.4%, with the pore structure characterized by a “macropores decreasing, micropores increasing” refinement pattern. After 18 wetting–drying cycles, the cumulative strength loss was 11.26%, outperforming cement-stabilized gravel. Combined with SEM observations, these performance improvements are attributed to the densely intertwined hydration products, particularly C-S-H gel, which effectively fill the voids between aggregate particles and significantly enhance the volume stability, freeze–thaw resistance, and wetting–drying durability of the stabilized gravel. The application of this cementitious material in a semirigid base course demonstrates excellent mechanical and durability properties, providing a theoretical basis and technical support for the widespread application of industrial solid waste in road engineering. Full article

Hydroxyapatite (HAp) is a widely used bioceramic in bone tissue engineering due to its biocompatibility and osteoinductive capacity; however, sustainable low-cost synthesis routes remain a challenge. This study evaluated HAp-polysaccharide composite synthesis from nejayote, the alkaline wastewater of maize nixtamalization, via chemical precipitation with (NH₄)₃PO₄ under controlled and uncontrolled pH, followed by calcination at 550 °C for 2 or 4 h. Controlled pH synthesis yielded higher solid recovery (89.8% vs. 76.4%), better calcium removal (99.8% vs. 87.4%), and smaller particle sizes (423.6 nm vs. 715.0 nm). XRD and FTIR confirmed HAp formation in both conditions, with crystallinity increasing upon calcination. Monomeric composition analysis revealed co-precipitation of amylose and arabinoxylan-derived polysaccharides in uncalcined samples, progressively eliminated by thermal treatment. Cell viability assays with human fetal osteoblasts (hFOB 1.19) confirmed non-cytotoxicity at all concentrations tested (10–633 μg/mL). Uncalcined composites synthesized without pH control achieved 126% cell viability at 633 μg/mL, surpassing pH-controlled and calcined counterparts (90–100%), suggesting active promotion of osteoblast proliferation, further supported by fluorescence imaging. These results establish nejayote as a viable dual source of calcium and polysaccharides for sustainable HAp composite synthesis with biomedical potential. Full article

Water-soluble blue microbial pigments with antioxidant activity remain rare, and their host-level protective mechanisms are poorly understood. Here, we identified the genetic basis of blue pigment biosynthesis in the glacier-derived strain Arthrobacter antioxidans QL17. Heavy-ion mutagenesis yielded a hyperpigmented mutant (M157) and a pigment-deficient mutant (M186), and pigment yield was positively associated with hydrogen peroxide (H₂O₂) tolerance. Genome mining identified MWM45_RS16760 as the sole core biosynthetic gene in a candidate nonribosomal peptide synthetase (NRPS)-like cluster. The encoded protein displayed an adenylation–peptidyl carrier protein–thioesterase (A-PCP-TE) architecture with a predicted L-glutamine-specific A domain, and its transcript abundance paralleled pigment production across the three strains. Phylogenetic analysis placed MWM45_RS16760 in a distinct actinomycete-associated indigoidine-like lineage separated from the characterized BpsA and IndC branches. Heterologous expression in Escherichia coli reconstructed a blue-pigment-producing phenotype, increased H₂O₂ tolerance, and was accompanied by enhanced extracellular DPPH and ABTS radical-scavenging activities in the culture supernatant. Comparative transcriptomics further revealed coordinated activation of oxidative-stress and proteostasis responses alongside repression of tryptophan biosynthesis and flagellar assembly. These findings identify MWM45_RS16760 as a candidate indigoidine-like NRPS associated with blue pigment biosynthesis and oxidative-stress resistance, with heterologous expression linked to enhanced radical scavenging and coordinated transcriptional reprogramming, expanding the phylogenetic and functional diversity of indigoidine-like systems. Full article

Artemisia annua contains artemisinin, a sesquiterpene lactone endoperoxide; artemether is a semisynthetic derivative of artemisinin that may offer potential advantages due to its redox-modulating and antimicrobial activities. These compounds have been associated with oxidative-stress modulation and microbial inhibition, making them promising candidates for experimental evaluation in nutritional and post-harvest quality studies. This study evaluated the effect of dietary supplementation with A. annua powder, artemisinin, and artemether on the refrigerated quality of Nile tilapia (Oreochromis niloticus) fillets. A total of 160 Nile tilapia were randomly assigned to four treatments: control (no additive), 1% A. annua powder, artemisinin (9.6 mg/kg feed), or artemether (9.6 mg/kg feed). After 30 days of feeding, 320 fillets were collected and stored under refrigeration at 4 °C. Samples were analyzed immediately after slaughter (day 0) and on days 7, 15, and 30. For each treatment group and sampling time, 20 fillets were used: 10 for microbiological evaluations, including counts of mesophilic and psychrotrophic bacteria, molds and yeasts, sulfite-reducing Clostridium, Enterobacteriaceae, coagulase-positive staphylococci, and coliforms; and 10 for physicochemical analyses, including pH, colorimetry, lipid oxidation through TBARS, and sensory evaluation. All supplemented treatments demonstrated improved microbial stability and lower TBARS values when compared with the control. Spoilage indicators such as discoloration, texture loss, and odor deterioration were also delayed. Artemether showed the most pronounced benefits, with lower microbial loads and oxidation indices for several evaluated parameters. These findings suggest that dietary supplementation with A. annua and its derivatives may help delay post-harvest quality deterioration of tilapia fillets during refrigerated storage. Full article