Search Results (5,678)

This study assessed the impact of cocrystal essential oil (CEO) inclusion on growth performance, nutrient digestibility, intestinal morphology, and antioxidant status in weaned piglets. Ninety-six weaned piglets were assigned to four groups (n = 8, three piglets per pen). The piglets in the four groups were fed basal diets added with 0, 120, 180, or 240 mg/kg of CEO, respectively, over a 28-day trial period. Results showed that during weeks 1–2, piglets in the 240 mg/kg CEO group exhibited a lower diarrhea rate and diarrhea index compared to the control group (p < 0.05). In weeks 3–4, the 180 and 240 mg/kg CEO groups demonstrated a reduced diarrhea rate and diarrhea index compared to the control group (p < 0.05). Relative to the control group, the apparent total tract digestibility (ATTD) of dry matter was elevated in piglets fed diets added with 120, 180, or 240 mg/kg CEO at both day 14 and day 28 (p < 0.05). Additionally, the ATTD of crude protein was elevated in the 120 mg/kg CEO group at day 14 and in the 180 mg/kg CEO group at day 28 (p < 0.05). Regarding intestinal morphology, supplementation with 180 or 240 mg/kg CEO increased jejunal villus height (VH) and the villus height to crypt depth (VH/CD) ratio compared with the control group (p < 0.05). Furthermore, 240 mg/kg CEO supplementation augmented the ileal VH/CD ratio relative to the control group (p < 0.05). For antioxidant status, 180 mg/kg CEO supplementation elevated serum glutathione peroxidase (GSH-Px) activity in piglets relative to the control group (p < 0.05). Importantly, no differences were found between the 180 mg/kg and 240 mg/kg CEO groups across all measured parameters (p > 0.05). In conclusion, dietary inclusion with 180 mg/kg CEO is recommended for weaned piglets, given its comprehensive benefits in alleviating diarrhea, improving nutrient digestibility, enhancing intestinal morphology, and bolstering antioxidant status. Full article

The objective of this paper is to offer a mathematical formulation of economic value added (EVA) that incorporates distance-to-default (DD) and thus a default-free capital structure. The latter is extended via the weighted average cost of capital (WACC) to introduce a default-free EVA. The data include the nonfinancial firms listed in the DJIA30 and NASDAQ100 covering the period 1992Q2–2023Q3. The results of standard specification tests and the GMM estimator show that (a) DD causes an increase in WACC and thus, EVA decreases; (b) the interest coverage ratio can be used effectively to compensate for default risk, thus adjusting the default-free EVA positively; (c) both EVA and default-free EVA can effectively be managed via common determinants, namely, net working capital ratio, total liabilities to EBITDA, sales growth rate, debt–equity ratio, and earnings per share; (d) the positive impact of the inflation rate on both EVA and default-free EVA justifies the use of default-free EVA as a metric for equity risk premium; and (e) the robustness of the results via stochastic geometric Brownian motion shows that the determinants of default-free EVA are stable. This paper contributes to related studies by incorporating credit risk via the DD into default-free EVA. Full article

Hollow-core anti-resonant fibers (HC-ARFs) have emerged as a promising platform for next-generation optical systems, offering attractive advantages in low-latency, low-nonlinearity, and high-power handling. However, the development of high-performance functional components, such as polarization beam splitters (PBSs), within this platform faces a significant challenge: the simultaneous achievement of ultra-broad bandwidth, compact device length, high polarization selectivity, and strict single-mode operation remains elusive. To address this challenge, we propose and numerically investigate a novel dual hollow-core anti-resonant fiber (DHC-ARF) based on a hybrid nodal–nodeless architecture. The design integrates three functional units: (1) an asymmetric nested semi-elliptical tube pair that defines the dual cores and serves as the primary wavelength-insensitive coupling channel; (2) nodeless nested circular tubes positioned peripherally to effectively suppress higher-order mode propagation while maintaining low fundamental mode loss; and (3) a selective localized thick-wall region that introduces a polarization-dependent perturbation to the x-polarized supermodes, whose observed behavior is physically consistent with a phase-mismatch effect associated with anti-crossing-like modal interaction near the target wavelength. Through synergistic optimization of these elements, we numerically demonstrate a combination of performance metrics. At the central wavelength of 1.55 µm, the coupling length for the y-polarization (Lcy) is reduced to 6.35 cm, while the coupling length ratio (CLR = $L_{\mathrm{cx}}$/$L_{cy}$) equals 2.001, indicating effective polarization selectivity. Consequently, a device length of 12.7 cm is numerically demonstrated, which is comparable to or shorter than existing ultra-broadband DHC-ARF PBS designs. The proposed PBS is numerically shown to exhibit an ultra-broad bandwidth of 460 nm (spanning 1320 to 1780 nm) with a polarization extinction ratio better than 20 dB, peaking at 53 dB. Furthermore, HOMER (λ) remains above 100 throughout the operating band and exceeds 200 over most of the band, indicating robust single-mode operation. This work not only presents a PBS design with competitive overall performance but also provides a versatile structural paradigm for developing functional components in hollow-core fiber-based integrated optical systems for high-speed communications and precision sensing. It should be noted that this work is based on numerical simulations, and experimental fabrication and validation will be pursued in future work. Full article

The increasing prevalence of cardiovascular diseases highlights the need to develop vascular grafts that match the mechanics of native vascular tissue and offer functional adaptability. This study reports the development and systematic optimization of a shape-memory polyurethane acrylate (PUA)-based photocurable resin for digital light processing (DLP)-based four-dimensional printing (4DP) applications. Resin formulations were designed by controlling hard/soft segment ratios, reactive diluent content, and crosslink density to position the glass transition temperature (T_g) within the physiological range (25–40 °C). Thermomechanical characterization was performed via dynamic mechanical analysis (DMA) and tensile testing, while a full-factorial Design of Experiments (DoE) approach was applied to optimize DLP process parameters—namely layer thickness, exposure time, and post-curing time. The developed resin formulation yielded a T_g of 38 °C as determined by DMA. Following process optimization, regression models showed high statistical fit (R² > 99%), and experimental validation under optimal conditions (layer thickness: 82.83 µm, exposure time: 11 s, post-curing: 2 min) resulted in an elongation at break of 64.0 ± 3.4%, a Young’s modulus of 10.9 ± 0.1 MPa, and a tensile strength of 6.2 ± 0.3 MPa. The optimized system exhibited thermally triggerable shape memory behavior at near-body temperature, with mechanical properties consistent with natural arterial tissue benchmarks. These findings demonstrate a promising material design strategy for DLP-based 4D-printed vascular structures. Full article

Background: Emerging evidence highlights the importance of the MIF–sCD74 axis in health and disease, including its role in regulating cell death. While studies in routine cardiac surgery suggest perioperative relevance, its role in end-stage heart failure (ESFH) patients undergoing left ventricular assist device (LVAD) implantation remains unexplored. Moreover, although MIF and sCD74 induce necroptosis in neonatal cardiac myofibroblasts, the effects of MIF, its paralog D-DT, and sCD74 on adult cardiac myofibroblasts (CMFs) are unknown. Methods: Plasma concentrations of sCD74, MIF and D-DT were measured perioperatively in a small cohort of patients with ESHF undergoing LVAD implantation (n = 20). As a preclinical model of ESHF, primary adult CMFs were treated with recombinant MIF, D-DT and sCD74 to evaluate their effects on cellular viability and health. Results: In LVAD patients, sCD74 and D-DT levels were significantly increased 24 h postoperatively, whereas MIF levels were reduced compared to baseline. ROC curve analysis demonstrated a good discriminatory power of 24 h post-OP sCD74 (AUC = 0.83), sCD74/MIF ratio (AUC = 0.82), and D-DT levels (AUC = 0.88) for acute kidney injury, composite outcome, and right heart failure (RHF), respectively. In adult CMFs, MIF and sCD74 synergistically reduced viable cell counts (p = 0.0083), whereas D-DT reduced cell counts in an sCD74-independent manner (p = 0.0004). Yet, measures of metabolism, proliferation, apoptosis and necrosis along with inflammatory gene expression remained unchanged. Conclusions: Our findings indicate that the balance of MIF, D-DT, and sCD74 during LVAD implantation may be clinically relevant. In particular, an imbalance characterized by elevated sCD74 or D-DT and reduced MIF levels 24 h post-surgery was associated with unfavorable clinical outcomes. Yet, the current findings are exploratory and hypothesis-generating because of a small sample size. Thus, the prognostic value of plasma levels for postoperative complications after LVAD implantation, and the effects of MIF/D-DT/sCD74 imbalance on cardiac myofibroblasts, need to be validated in larger cohorts and in advanced human experimental models. Full article

Background: Long noncoding RNAs (lncRNAs) have emerged as critical regulators of hepatic metabolism and disease progression. The hepatocyte nuclear factor 1 alpha antisense 1 (HNF1A-AS1) lncRNA modulates liver-specific transcription factors; however, its physiological role in diet-dependent lipid homeostasis remains poorly defined. Methods: In this study, we investigated the mouse ortholog, Hnf1a opposite strand 1 (Hnf1aos1), using AAV-mediated knockdown in C57BL/6J mice fed either a chow diet (10% kcal from fat) or a high-fat diet (HFD; 60% kcal from fat) for 12 weeks. Metabolic phenotyping included hepatic lipid quantification, histological analysis, serum biochemistry, and quantitative gene expression profiling. Results: Loss of Hnf1aos1 produced distinct, diet-dependent alterations in hepatic lipid handling. Under chow conditions, knockdown mice exhibited selective hepatic cholesterol accumulation (6.10 ± 2.9 mg/g tissue vs. 3.51 ± 1.1 mg/g in controls), accompanied by dysregulation of cholesterol clearance pathways. In contrast, under HFD conditions, knockdown precipitated severe macrovesicular degeneration, with hepatic triglyceride levels approximately doubled relative to HFD-fed controls (51.72 ± 19.8 mg/g vs. 26.34 ± 11.9 mg/g) and a numerically elevated triglyceride-to-cholesterol ratio (TG:TC ≈ 6.1:1; p = 0.0621, trend). Chow/Kd mice gained significantly less weight than chow-fed controls, whereas HFD/Kd mice exhibited weight gain comparable to HFD controls despite severe hepatic steatosis. This paradoxical phenotype suggests impaired metabolic feedback at the post-transcriptional level, in which compensatory upregulation of Hnf1a mRNA is insufficient to suppress lipid-associated genes such as Cd36, despite profound lipid overload; however, HNF1A protein levels were not directly measured in this study. Conclusion: Collectively, these findings identify Hnf1aos1 as a regulator of hepatic lipid homeostasis whose loss produces a phenotype consistent with inappropriate lipid accumulation during nutrient excess, without defining the underlying molecular mechanism. Our results support a role for Hnf1aos1 in shaping hepatic metabolic plasticity and provide insight into lncRNA-associated MASLD phenotypes. Full article

To address the challenges of inefficient depolymerization and undesirable condensation side reactions of lignin in lignocellulosic biomass, this study employed an ethanolamine pyruvate protic ionic liquid (EAP) pretreatment system to achieve selective separation of lignin from eucalyptus while simultaneously enabling its in situ structural activation. Under optimized conditions (pyruvate-to-ethanolamine mole ratio of 1:3, 120 °C, 40 min), the EAP system afforded a lignin separation yield of 79.0 ± 0.6%, with dissolution yields of cellulose and hemicellulose of 9.6 ± 0.3% and 11.2 ± 0.4%, respectively. According to 2D-HSQC NMR and ³¹P NMR analyses, the relative content of β-O-4 ether linkages in the isolated lignin decreased from 18.4 ± 0.4% to 14.2 ± 0.3% after EAP treatment. The total phenolic hydroxyl content reached 2.26 ± 0.08 mmol/g, and the syringyl-to-guaiacyl (S/G) ratio declined from 1.72 ± 0.04 to 0.71 ± 0.03. Based on these observations, it is proposed that the ethanolamine component facilitates the dissociation of the lignin network through hydrogen bonding and stabilizes reactive intermediates, while the pyruvate component participates in the cleavage of β-O-4 ether linkages and the removal of methoxy groups via proton catalysis and nucleophilic attack. Compared with the ethanolamine and ethanolamine acetate systems, EAP pretreatment yielded lignin of higher purity (98.4 ± 0.3%) under milder conditions, and the isolated lignin exhibited stronger antioxidant activity (IC₅₀ = 0.17 ± 0.02 mg/mL). This work offers insights into the development of pretreatment systems that combine efficient separation with structural preservation of lignin. Full article

As space exploration activities and strategic deployments in polar regions continue to advance, higher demands have been placed on the low-temperature resistance of propellant binders. Here, high cis-1,4 content hydroxyl-terminated polybutadiene (HTPB) was successfully synthesized via an oxidative cleavage method using commercial cis-polybutadiene (BR). The microstructure, molecular weight, hydroxyl value, rheological behavior, thermal properties, and mechanical performance of the resulting cis-HTPB were systematically characterized. By adjusting the molar ratio of mCPBA to butadiene units, three cis-HTPB samples with varying molecular weights were obtained. The high cis-1,4 structure (93%) was preserved after modification. The synthesized cis-HTPB exhibited an ultra-low glass transition temperature (Tg) of approximately −100 °C and lower viscosity compared to commercial HTPB, indicating excellent low-temperature flexibility and processability. In addition, the cis-HTPB was further modified with acrylate groups to produce a UV-curable derivative (AcTPB). The cured AcTPB also retained a Tg near −100 °C, demonstrating its suitability for ultra-low-temperature applications and its promise as a photocurable binder for 3D printing propellant. Full article

(1) Background: Carvedilol is a poorly water-soluble drug, which limits its therapeutic performance. Deep eutectic solvents (DES) and cyclodextrins (CD) are emerging solubilizing agents that can improve drug bioavailability. (2) Methods: Twenty-one DES were prepared using choline chloride and polyols or sugars (xylitol, sorbitol, glucose, and fructose) at different molar ratios with water. α and β cyclodextrins (CD) were added (0.5–2 mM) using two incorporation strategies: (Method 1) addition to the aqueous phase before DES formation; (Method 2) direct addition to the preformed DES. (3) Results: Carvedilol solubility markedly increased with DES–CD combinations. In Method 1, xylitol-based DES provided up to a 16-fold enhancement, especially with β-CD at low concentrations, while glucose and sorbitol systems showed modest effects. Fructose-based mixtures improved mainly at a 2:1:35 ratio without CDs. In Method 2, α-CD with xylitol or sorbitol yielded the highest increases (up to 38.9-fold). (4) Conclusions: The solubilization efficiency depends on DES composition, CD type, and concentration. α-CD combined with xylitol-based DES showed the best results, highlighting this approach as a promising strategy to enhance carvedilol solubility for pharmaceutical applications. Full article

(1) Background: Despite the success of combination antiretroviral therapy (cART), immune recovery in treated HIV infection remains heterogeneous, and discordant immune–virologic responses persist in a substantial proportion of people living with HIV (PLWH). These patterns may reflect ongoing immune dysregulation despite effective viral suppression. This study aimed to characterize discordant treatment classifications, evaluate immune imbalance using the CD4/CD8 ratio, identify associated clinical predictors, and assess opportunistic infection burden in a Romanian cohort of people living with HIV receiving long-term cART. (2) Methods: A retrospective cross-sectional study was conducted in 462 adults with HIV-1 infection receiving cART at the “Prof. Dr. Matei Balș” National Institute of Infectious Diseases, Bucharest (2018–2021). PLWH were classified as concordant responders (CR), immunological discordant responders (ID), or virological discordant responders (VD) based on plasma HIV-1 RNA and CD4+ T-cell count thresholds. Immune dysregulation was assessed using the CD4/CD8 ratio. Multinomial logistic, logistic, and negative binomial regression models were used to identify predictors of discordant responses, severe CD4/CD8 ratio inversion, and opportunistic infection burden. (3) Results: Discordant responses were observed in 30.7% of PLWH (14.5% ID, 16.2% VD). CD4/CD8 ratio inversion occurred in 71.2% and severe inversion in 40.0%. Significant differences across clinical classification groups were found for CD4+T-cell counts (H = 153.62, p < 0.001, ε² = 0.33) and CD4/CD8 ratio (H = 115.10, p < 0.001, ε² = 0.25), while CD8+ counts were similar (p = 0.571). Male sex was associated with both ID and VD, and severe CD4/CD8 inversion was strongly associated with ID. Opportunistic infection burden was associated with duration of HIV infection and CDC stage. (4) Conclusions: Discordant immune–virologic responses remain frequent during long-term cART and are characterized by persistent immune imbalance reflected by CD4/CD8 ratio inversion. The CD4/CD8 ratio may provide clinically relevant information on immune recovery beyond CD4+ T-cell counts. Full article

Carbon fiber-reinforced polymer (CFRP) laminates are susceptible to barely visible impact damage (BVID) under low-energy bird-strike-like conditions. However, in previous studies, most damage evaluations for BVID were limited to a single scale. In this work, a multiscale characterization and evaluation method integrating the analytic hierarchy process (AHP) and the CRITIC weighting method was proposed to investigate the damage evolution of CFRP laminates under low-energy impacts (approximately 12–33 J). Delamination area (S_Da), indentation depth (P_D), surface crack aspect ratio (R_A), energy dissipation, and compression-after-impact (CAI) strength were analyzed based on phased-array ultrasonic C-scanning, 3D optical profilometry, and scanning electron microscopy. The results showed that P_D, S_Da, and energy dissipation increased from 108.73 μm to 213.93 μm, from 228.6 mm² to 695.8 mm², and from 5.96 J to 21.40 J, respectively, with increasing impact energy. Meanwhile, CAI strength decreased from 202.2 MPa to 118.9 MPa, with a maximum degradation rate of 41.16%. A critical transition was observed in the medium-to-high energy range, where delamination growth gradually plateaued, while intralaminar cracking and fiber fracture became increasingly dominant. The proposed framework enables quantitative grading of BVID severity and provides a practical basis for assessing residual damage in impacted CFRP laminates. Full article

Background: High-grade soft tissue sarcomas (STSs) are heterogeneous tumours lacking robust prognostic or predictive biomarkers. Regnase-1, an immune RNase, enhances antitumour immunity by limiting immunosuppressive tumour microenvironment (TME) components (e.g., myeloid-derived suppressor cells (MDSCs)), but remains unexplored in STS. As CD68⁺ tumour-associated macrophages (TAMs) drive TME suppression and poor prognosis in non-translocation-driven STS, we evaluated Regnase-1 and CD68⁺ TAMs to assess Regnase-1 as an indicator of an immunologically activated TME. Methods: Immunohistochemistry scoring of Regnase-1 and CD68⁺ TAMs was performed in 91 patients. Overall survival (OS) was assessed by Kaplan–Meier and Cox regression, and findings were validated in an independent “The Cancer Genome Atlas” Sarcoma (TCGA-SARC) cohort (n = 212). Results: In UPS, Regnase-1-high predicted longer OS (17.0 months vs. not reached; p = 0.0247) and lower mortality (univariate hazard ratio (HR) = 0.3; p = 0.0343; multivariate HR = 0.4; p = 0.0413), but not after radiotherapy. CD68⁺ TAM-high predicted shorter OS (13.0 months vs. not reached; p = 0.0274) and higher mortality (HR = 2.0, 95% CI 1.1–3.7; p = 0.0325). Both Regnase-1 effects were reproduced in TCGA-SARC. Regnase-1-high tumours showed inflammatory/interferon enrichment, reduced TGF-β signalling, and SERPINE1 upregulation. Conclusions: Regnase-1 marked a pro-inflammatory TME and favourable outcome in UPS, but this effect may reverse upon radiotherapy. Full article

This paper presents a numerical investigation of vortex-induced vibration (VIV) of six elastically mounted circular cylinders in oscillatory flow, three smooth and three biofouled with triangular surface roughness elements. The study aims to characterise the influence of the longitudinal spacing ratio ($L/D=3,4,$ and $5$) on the two-degree-of-freedom (2DOF) vibration response at a constant Keulegan–Carpenter number of $KC=10$. Simulations are performed using the transient RANS equations with the SST $k–\omega$ turbulence model, and structural motion is resolved using a dynamic mesh approach. Lock-in behaviour is observed over the reduced velocity range $5\le U_r\le 10$. Biofouled cylinders generally exhibit higher in-line displacement amplitudes than smooth cylinders in the initial and lower lock-in branches, whereas smooth cylinders tend to attain higher in-line amplitudes in the upper lock-in branch. The spacing ratio $L/D$ is found to significantly influence the response, with peak vibration amplitudes varying non-uniformly across the array and no single spacing configuration being optimal for all cylinders. This behaviour is further supported by analyses of trajectories, frequency content, and vorticity fields. Among the smooth cylinders, the middle cylinder exhibits the largest in-line displacement amplitude of $3.28D$ at $L/D=5$ and the largest cross-flow displacement of $1.34D$ at $L/D=3$. For the biofouled configurations, the middle and upstream cylinders show the highest in-line displacement amplitude of $2.69D$ at $L/D=4$, while the maximum cross-flow displacement of $1.27D$ is observed for the upstream cylinder at $L/D=5$. Full article

To enhance the utilization of industrial coal gangue, response surface methodology was used to optimize the concrete mix proportions based on three key factors: the mass ratio of fly ash (FA) to metakaolin (MK) (A), the combined dosage of FA and MK (B), and the water-to-binder ratio (C). A quadratic regression model was established, and the optimal mixture was characterized using FT-IR, XRD, and SEM. The model exhibited high statistical significance (p < 0.001) and an excellent fit (R² > 0.95), confirming its predictive reliability. Single-factor analysis revealed that the order of influence on 28 d compressive strength was C > A > B, indicating that the water-to-binder ratio had the most significant effect on later-age strength. The optimal mix proportions were determined as follows: fly ash-to-MK ratio of 0.65, admixture dosage of 20% by mass of total binder, and C of 0.475. Under these conditions, the measured 28 d compressive strength reached 35.9 MPa, which was within 5% of the model-predicted value, thereby validating the model’s accuracy. Microstructural analysis demonstrated that the appropriate incorporation of FA and MK promoted the formation of C-S-H gel, refined the pore structure, and improved the quality of the interfacial transition zone, which collectively enhanced the mechanical performance. A systematic understanding of the strength and microstructural mechanisms of concrete incorporating coal gangue, fly ash, and metakaolin is currently lacking, which hinders the design of more robust and durable structures. This study addresses this gap by systematically clarifying the individual and combined effects of the key variables on the strength of coal gangue concrete. The findings reveal the underlying mechanisms, providing a scientific basis for the sustainable, large-scale application of coal gangue concrete in construction. Full article

Senecavirus A (SVA) has rapidly emerged as a globally distributed swine pathogen, with clinical signs mimicking vesicular diseases such as Foot-and-Mouth Disease, posing challenges for timely detection and control. Here, we analyzed 329 complete SVA genomes spanning multiple continents to provide a comprehensive view of its evolutionary dynamics, recombination patterns, haplotype diversity, and global dissemination. Phylogenetic analyses revealed two major lineages: Lineage 1, consisting mainly of early strains from the United States before 2007, and Lineage 2, which emerged post-2007 and subsequently spread across the Americas and East Asia. Recombination was confined to Lineage 2 and concentrated in nonstructural regions, particularly 2C, highlighting intra-lineage genetic exchange as a driver of recent diversification. Haplotype analysis of the 3AB gene identified 170 distinct haplotypes, revealing a star-like network structure consistent with rapid population expansion from a central ancestral variant, while secondary branches reflect ongoing regional diversification. Despite this high genetic variation, genome-wide dN/dS ratios remained below one, and purifying selection was strongest in the N-terminal domains of structural and nonstructural proteins, indicating functional constraints that maintain viral fitness. Time-scaled phylogenetic reconstruction and Bayesian Skyline analysis revealed rapid lineage diversification and a marked increase in effective population size in the early 2010s. Phylogeographic inference further identified repeated introductions from the Americas into East Asia, likely facilitated by swine trade and other anthropogenic factors. Collectively, SVA evolution is driven by frequent mutation and intra-lineage recombination yet constrained by pervasive purifying selection, generating extensive genetic diversity while maintaining functional integrity, with implications for genomic surveillance and targeted control. Full article