Special Issue "Proteomes of Extracellular Vesicles"

A special issue of Proteomes (ISSN 2227-7382).

Deadline for manuscript submissions: 31 March 2019

Special Issue Editors

Guest Editor
Prof. Piotr Widlak

Maria Sklodowska-Curie Institute-Oncology Center, Gliwice Branch, 44-101 Gliwice, Poland
Website | E-Mail
Interests: cancer biomarkers; cellular signaling; clinical proteomics; clinical metabolomics; mass spectrometry imaging; stress response
Guest Editor
Prof. Theresa L. Whiteside

Department of Pathology, University of Pittsburgh School of Medicine, PA, USA
Website | E-Mail
Interests: clinical cancer immunology; cancer immunotherapy; exosomes as cancer biomarkers; extracellular vesicles; immuno-oncology; immune suppression in cancer; regulatory T cells; tumor-derived exosomes; tumor microenvironment
Guest Editor
Prof. Monika Pietrowska

Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Branch in Gliwice, 44-101 Gliwice, Poland
Website | E-Mail
Interests: clinical proteomics; extracellular vesicles; immunoproteomics; mass spectrometry; radiobiology; radioproteomics; stress response

Special Issue Information

Most cells release different types of vesicles into the extracellular microenvironment, both in vivo and in vitro, which are known under the general term of “extracellular vesicles (EVs)”. Recently, much attention has been paid to a subset of small EVs called exosomes, because of their role in cell-to-cell communication and involvement in disease-related processes. Exosomes are 30–150nm in size and are membrane-enclosed structures. They are actively secreted by cells via the mechanisms involving multivesicular bodies (MVBs). They transmit complex molecular and genetic information, including signals for cell death, survival, and differentiation, from exosome-secreting cells to multiple types of neighboring or distant recipient cells. The functional role of EVs depends on their molecular cargo, including their proteome content. EVs present in blood and other physiological fluids have a large potential for liquid biopsy and are an emerging source of disease biomarkers. However, the isolation and characterization of EVs, including the analysis of their proteome cargo, remains a challenge and represents an important limitation in this field of research.

We invite you to contribute original research, technical notes, methods papers, and reviews on the subject of the “Proteomes of Extracellular Vesicles”. Papers that cover advances in the methods of isolation and analysis of these vesicles by mass spectrometry approaches as well as their implementation both in basic research and the translational field of disease proteomics will be appreciated by our readership and will be highlighted as important and significant advancements of proteomic science.

Prof. Piotr Widlak
Prof. Theresa L. Whiteside
Prof. Monika Pietrowska
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Proteomes is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 550 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biomarkers
  • cancer cell biology
  • exosomes
  • extracellular vesicles
  • human proteome
  • immune cell regulation
  • liquid biopsy
  • mass spectrometry
  • stress response

Published Papers

This special issue is now open for submission, see below for planned papers.

Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: Comparison of Extracellular Vesicle Yields from Different Body Fluids for Proteomic Studies
Authors: Helena Kupcova Skalnikova et al.
Affiliation: Laboratory of Applied Proteome Analyses, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburska 89, Libechov, Czech Republic
Email:
Abstract: Extracellular vesicles (EVs) are highly attractive objects in biomedical research as possible carriers of nucleic acid and protein biomarkers. EVs released to body fluids are extremely valuable as they may enable us accession of inner organs by so called “liquid biopsy”. However, the EV content in various body fluids largely differs, which may hamper subsequent proteomic analyses. Obtaining a high quality EV sample with minimum contaminants is crucial for proteomic analyses using LC-MS/MS or other techniques. We will present a comparison of extracellular vesicle yields from various body fluids (blood plasma, cerebrospinal fluid, seminal plasma) of experimental pig model for proteomic analysis. Pigs are widely used in biomedical research as large animal models with anatomy and physiology close to human and enable studies (e.g. of the nervous system) that are unfeasible in human. The EVs will be isolated from body fluids by differential centrifugation followed by ultracentrifugation and characterized not only according to protein yields, but also according to the quality of isolated vesicles (e.g. size distributions, morphology, positivity for exosomal markers). We believe that this comparison will be useful for broad range of readers and may facilitate design of proteomic experiments employing EVs isolated from mammalian, including human, body fluids.

Title: Melanoma-derived extracellular vesicles: focus on the proteome
Authors: Magdalena Surman, Ewa Stępień and Małgorzata Przybyło
Affiliation: Department of Glycoconjugate Biochemistry, Institute of Zoology, Jagiellonian University, 30‐387 Kraków, ul. Gronostajowa 9, Poland
Email: [email protected]
Abstract: Malignant melanoma is one of the most aggressive types of cancer, and its incidence is increasing rapidly each year. Despite the extensive research into improved diagnostic and treatment methods, early detection and disease constraint still present with significant challenges. As successful isolation protocols developed, extracellular vesicles (EVs) became increasingly investigated in terms of their role in cancer progression and as a possible source of disease biomarkers. Besides functional studies, quantitative and qualitative proteomics have recently emerged as promising tools for the advancement of melanoma biomarkers. Nevertheless, the amount of data concerning the proteome of melanoma-derived EVs is still very limited. In this review we cover the current knowledge on protein content of melanoma-derived EVs, with focus on its potential role in development and progression of melanoma.

Title: Extracellular vesicle integrins distinguish unique breast cancers
Authors: David G. Meckes Jr and Stephanie N. Hurwitz
Affiliation: Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USA
Email: [email protected]
Abstract: The proteomic profile of extracellular vesicles (EVs) has been of increasing interest, particularly in understanding cancer growth, drug resistance, and metastatic behavior. Emerging data suggests that cancer-derived EVs may carry an array of oncogenic cargo, including certain integrin proteins that may, in turn, promote cell detachment, migration, and selection of future metastatic sites. We previously reported a large comparison of secreted vesicle protein cargo across sixty diverse human cancer cell lines. Here, we analyze the distinct integrin profiles of these cancer EVs. We further demonstrate the enrichment of integrin receptors in breast cancer EVs compared to vesicles secreted from benign breast epithelial cells. Total EV integrin levels, including the quantity of integrins alpha-2, alpha-V, beta-4, and beta-5 correlate with breast tumor stage. In particular, integrin alpha-2 also largely reflects progenitor cell expression, highlighting the utility of this integrin protein as a potential circulating biomarker of primary tumors. This study provides preliminary evidence of the value of vesicle-associated integrin proteins in cancer diagnosis and prediction of tumor stage. Differential expression of integrins across cancer cells, and selective packaging of integrins into EVs may contribute to further understanding the development and progression of tumor growth and metastasis across a variety of cancer types.

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