Isolation and Characterization of Small Extracellular Vesicles from Porcine Blood Plasma, Cerebrospinal Fluid, and Seminal Plasma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. Isolation of EVs from Blood Plasma
2.3. Isolation of EVs from Cerebrospinal Fluid
2.4. Isolation of EVs from Seminal Plasma
2.5. Flow Cytometry
2.6. Electron Microscopy
2.7. Protein Extraction and Western Blotting
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kupcova Skalnikova, H.; Bohuslavova, B.; Turnovcova, K.; Juhasova, J.; Juhas, S.; Rodinova, M.; Vodicka, P. Isolation and Characterization of Small Extracellular Vesicles from Porcine Blood Plasma, Cerebrospinal Fluid, and Seminal Plasma. Proteomes 2019, 7, 17. https://doi.org/10.3390/proteomes7020017
Kupcova Skalnikova H, Bohuslavova B, Turnovcova K, Juhasova J, Juhas S, Rodinova M, Vodicka P. Isolation and Characterization of Small Extracellular Vesicles from Porcine Blood Plasma, Cerebrospinal Fluid, and Seminal Plasma. Proteomes. 2019; 7(2):17. https://doi.org/10.3390/proteomes7020017
Chicago/Turabian StyleKupcova Skalnikova, Helena, Bozena Bohuslavova, Karolina Turnovcova, Jana Juhasova, Stefan Juhas, Marie Rodinova, and Petr Vodicka. 2019. "Isolation and Characterization of Small Extracellular Vesicles from Porcine Blood Plasma, Cerebrospinal Fluid, and Seminal Plasma" Proteomes 7, no. 2: 17. https://doi.org/10.3390/proteomes7020017