Applications of Microorganisms in Wastewater Treatment

A Special Issue of Processes (ISSN 2227-9717) belonging to the section "Environmental and Green Processes".

Deadline for manuscript submissions: closed (20 August 2026) | Viewed by 1772

Editors


E-Mail Website
Guest Editor
Applied Microbial and Health Biotechnology Institute, Cape Peninsula University of Technology, Cape Town 7535, South Africa
Interests: microbial bioplastics; bioremediation and valorization of waste and wastewater; algal biofuel

E-Mail Website
Guest Editor
Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
Interests: enzymatic technology; biofuels; wastewater treatment
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Microorganisms are vital to wastewater treatment due to their remarkable metabolic diversity, flexibility, and ability to translate pollutants into safe or reusable forms for the natural environment. Due to continuous demands and challenges in energy requirements, operational expenses, and restricted efficiency in eliminating complex pollutants, traditional treatment technologies have driven renewed interest towards microbial-based processes as sustainable, nature-inspired alternatives.

This Special Issue, “Applications of Microorganisms in Wastewater Treatment,” will focus on recent developments in employing bacteria, microalgae, fungi, and mixed microbial communities for treating municipal, industrial, agricultural, and other diverse types of wastewater. This research will support essential processes like organic matter degradation, removal of heavy metals and inhibitory compounds, feedstock pretreatment, microbial immobilization, nutrient bioaccumulation, and the biochemical mechanisms underlying microbial remediation. It also explores diverse waste and wastewater valorization pathways, microbial co-culturing strategies, biofilm reactors, aerobic granular sludge, growth kinetics, modeling studies, and the biotransformation of emerging pollutants. Focus is directed toward novel system architectures, microbial relationships, metabolic pathways, and process optimization approaches that enhance treatment efficiency, stability, and reliability.

This Special Issue combines experimental, analytical, and modeling research to shed light on the mechanisms that dictate microbial performance and advance resource-efficient and circular approaches to wastewater management. The insights provided here aim to guide researchers, practitioners, and policymakers involved in creating sustainable wastewater treatment solutions as well as support the global move toward a circular bioeconomy and environmental resilience.

Dr. Amrita Ranjan
Prof. Dr. Luqman Chuah Abdullah
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-anonymized peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • wastewater
  • bioremediation
  • microbial cultures
  • consortium
  • valorization
  • microbial biochemistry
  • kinetic and modeling study
  • statistical optimization
  • circular economy
  • sustainability
  • industrial microbiology

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

18 pages, 1150 KB  
Article
Measurement of Biomass in Small-Scale Microalgal and Microalgal–Bacterial Systems for Wastewater Treatment: Mini Review and Experimental Evaluation
by Amrita Ranjan, Philadelphia V. Ngobeni and Pamela Jean Welz
Processes 2026, 14(7), 1145; https://doi.org/10.3390/pr14071145 - 2 Apr 2026
Cited by 2 | Viewed by 963
Abstract
Accurate biomass quantification is important for evaluating growth kinetics and performance of microalgal and microalgal–bacterial wastewater treatment systems. However, small-scale studies frequently encounter methodological limitations due to low biomass concentrations, limited sampling volumes, and/or interference from non-biotic solids in complex wastewaters. This work [...] Read more.
Accurate biomass quantification is important for evaluating growth kinetics and performance of microalgal and microalgal–bacterial wastewater treatment systems. However, small-scale studies frequently encounter methodological limitations due to low biomass concentrations, limited sampling volumes, and/or interference from non-biotic solids in complex wastewaters. This work adopts a two-fold approach: (i) a concise review of current biomass quantification methods for bench-scale systems, and (ii) an experimental evaluation of a gravimetric protocol for complex wastewaters. The review discusses commonly applied techniques, highlights their strengths and weaknesses, and identifies research gaps in data comparability and reproducibility. The laboratory investigations evaluated the effects of key factors, namely culture volume (250 mL to 1 L), test aliquots (2.5 mL to 10 mL), and the absolute weight of total suspended solids (3.43 g to 14.5 g) on total suspended solids measurements. Aliquots containing <5 mg total suspended solids produced statistically significant variability, whereas reliable and reproducible results were obtained when >8–10 mg absolute total suspended solids per aliquot was present. In complex wastewater matrices, approximately 18% of total suspended solids consisted of non-volatile solids, demonstrating that the method can systematically over-estimate true dry cell weight in microalgal–bacterial systems. The findings emphasized the need for procedural standardization. Finally, a practical gravimetric protocol is proposed for both axenic and consortium-based small-scale studies dealing with complex wastewater, providing an evidence-based roadmap for obtaining more reliable biomass estimations. Full article
(This article belongs to the Special Issue Applications of Microorganisms in Wastewater Treatment)
Show Figures

Graphical abstract

Back to TopTop