Metabolomics in Medicinal Plants and Agricultural Research

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Physiology and Metabolism".

Deadline for manuscript submissions: closed (31 August 2024) | Viewed by 2361

Special Issue Editors


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Guest Editor
Agricultural Research Station, Qatar University, Doha, Qatar
Interests: bioactives; nutraceuticals; food chemistry; chromatography; mass spectrometry; metabolomics
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Plant Science and Technology, Chung-Ang University, Anseong-si 17546, Gyeonggi-do, Republic of Korea
Interests: phytochemicals; metabolomics; horticultural crop quality

Special Issue Information

Dear Colleagues,

Metabolomics is becoming a valuable analytical tool in plant research. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) are commonly used analytical techniques in plant metabolomics. Both MS- and NMR-based metabolomics approaches have been effectively used for the qualitative and quantitative evaluation of plant metabolites or phytochemicals, especially those present in medicinal plants as well as those involved in plant–biotic/abiotic interactions. In natural product research, metabolomics is a valuable tool in discovering novel drug candidates and proving their bio-efficacy as novel drug candidates under in vivo and in vitro conditions. In the fields of plant biology and crop improvement, metabolomic tools have been applied to the qualitative and quantitative evaluations of plant traits, including understanding the role of the metabolites involved in plant–biotic/abiotic interactions. The application of plant metabolomics in plant biology and medicinal plant-based drug discovery is ongoing, with rapid improvements in analytical and data processing tools. The goal of this Special Issue is to collect the recent highlights and reviews associated with the application of plant metabolomics. Submissions related to the MS- or NMR-based characterization of plant metabolites, targeted and untargeted (MS or NMR) metabolomics studies, phytochemistry of medicinal plants, methods, and protocols used in plant metabolomics are welcome.

Dr. Deepak M. Kasote
Dr. Jisun H. J. Lee
Guest Editors

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Keywords

  • mass spectrometry
  • metabolomics
  • plant interactions (biotic and abiotic)
  • natural products
  • medicinal plants

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Published Papers (1 paper)

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Research

24 pages, 14977 KiB  
Article
Metabolite and Transcriptomic Changes Reveal the Cold Stratification Process in Sinopodophyllum hexandrum Seeds
by Rongchun Ning, Caixia Li, Tingting Fan, Tingting Ji and Wenhua Xu
Plants 2024, 13(19), 2693; https://doi.org/10.3390/plants13192693 - 26 Sep 2024
Viewed by 1867
Abstract
Sinopodophyllum hexandrum (Royle) Ying, an endangered perennial medicinal herb, exhibits morpho-physiological dormancy in its seeds, requiring cold stratification for germination. However, the precise molecular mechanisms underlying this transition from dormancy to germination remain unclear. This study integrates transcriptome and plant hormone-targeted metabolomics techniques [...] Read more.
Sinopodophyllum hexandrum (Royle) Ying, an endangered perennial medicinal herb, exhibits morpho-physiological dormancy in its seeds, requiring cold stratification for germination. However, the precise molecular mechanisms underlying this transition from dormancy to germination remain unclear. This study integrates transcriptome and plant hormone-targeted metabolomics techniques to unravel these intricate molecular regulatory mechanisms during cold stratification in S. hexandrum seeds. Significant alterations in the physicochemical properties (starch, soluble sugars, soluble proteins) and enzyme activities (PK, SDH, G-6-PDH) within the seeds occur during stratification. To characterize and monitor the formation and transformation of plant hormones throughout this process, extracts from S. hexandrum seeds at five stratification stages of 0 days (S0), 30 days (S1), 60 days (S2), 90 days (S3), and 120 days (S4) were analyzed using UPLC-MS/MS, revealing a total of 37 differential metabolites belonging to seven major classes of plant hormones. To investigate the biosynthetic and conversion processes of plant hormones related to seed dormancy and germination, the transcriptome of S. hexandrum seeds was monitored via RNA-seq, revealing 65,372 differentially expressed genes associated with plant hormone synthesis and signaling. Notably, cytokinins (CKs) and gibberellins (GAs) exhibited synergistic effects, while abscisic acid (ABA) displayed antagonistic effects. Furthermore, key hub genes were identified through integrated network analysis. In this rigorous scientific study, we systematically elucidate the intricate dynamic molecular regulatory mechanisms that govern the transition from dormancy to germination in S. hexandrum seeds during stratification. By meticulously examining these mechanisms, we establish a solid foundation of knowledge that serves as a scientific basis for facilitating large-scale breeding programs and advancing the artificial cultivation of this highly valued medicinal plant. Full article
(This article belongs to the Special Issue Metabolomics in Medicinal Plants and Agricultural Research)
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