Biotechnological Intervention and Secondary Metabolite Production in Plants

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 984

Special Issue Editors


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State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Centre for Sustainable Forestry in Southern China, Bamboo Research Institute, Key Laboratory of National Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, School of Life Sciences, Nanjing Forestry University, Nanjing 210037, China
Interests: genetic transformation; medicinal plant; secondary metabolites; somatic embryogenesis; transcriptomics
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Guest Editor
Plant Biotechnology Section, Department of Botany, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India
Interests: genetic transformation; medicinal plant; secondary metabolites; organogenesis; somatic embryogenesis; transcriptomics

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Guest Editor
Bamboo Research Institute, School of Life Sciences, Nanjing Forestry University, Nanjing 210037, China
Interests: bamboo epigenetic; bamboo rapid growth; bamboo genomics; stress tolerance, RNA and DNA modification; transposons; sustainable development
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The production of secondary metabolites in plants is vital for pharmaceutical, agricultural, and industrial applications, yet traditional methods face challenges like low yield, seasonal dependence, and environmental fluctuations.

Biotechnological interventions offer innovative solutions to these challenges by harnessing cutting-edge tools such as genetic engineering, tissue culture, and metabolic pathway optimization. Advances in CRISPR-Cas technology, synthetic biology, in vitro culture techniques, and elicitation strategies have revolutionized secondary metabolite enhancement, enabling the precise manipulation of biosynthetic pathways and sustainable production.

This Special Issue studies the latest advancements in biotechnological interventions for enhancing secondary metabolite production in plants. Central to this exploration are genome-editing technologies, such as CRISPR-Cas, which offer precise tools to upregulate biosynthetic pathways and fine-tune metabolite synthesis. The roles of elicitors, nanoparticles, and omics-driven approaches are also highlighted for their potential to boost metabolite yield and scalability while ensuring sustainability. Using tissue-specific and inducible promoters is a promising strategy to optimize metabolite accumulation without compromising plant growth or development.

Additionally, this Special Issue addresses the pressing question of identifying sustainable and scalable platforms, such as plant cell cultures or engineered microbial systems, for industrial-scale production. By addressing these critical advancements, this Special Issue will revolutionize secondary metabolite production for applications in plant biotechnology and beyond. The insights gathered will contribute to eco-friendly, efficient, and scalable solutions that integrate seamlessly into plant biotechnology and industrial applications.

Dr. Zishan Ahmad
Prof. Dr. Anwar Shahzad
Prof. Dr. Muthusamy Ramakrishnan
Guest Editors

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Keywords

  • CRISPR/Cas technology
  • elicitation
  • plant tissue culture
  • plant bio factories
  • metabolic engineering
  • secondary metabolites
  • synthetic biology

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Published Papers (1 paper)

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Research

23 pages, 6512 KB  
Article
Micropropagation of Ajuga bracteosa via Direct Organogenesis Using Internodal Explants: SEM, GC-MS, and SCoT Marker Analysis
by Arisha Kausar, Anwar Shahzad, Aashiq Yousuf Bhat, Muthusamy Ramakrishnan and Zishan Ahmad
Plants 2025, 14(16), 2507; https://doi.org/10.3390/plants14162507 - 12 Aug 2025
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Abstract
Ajuga bracteosa is a herb with high medicinal value and a low range of distribution. It is used in several herbal and traditional medicines, including diabetes. In the present study, we designed the methodology for the micropropagation of A. bracteosa from internodal segments. [...] Read more.
Ajuga bracteosa is a herb with high medicinal value and a low range of distribution. It is used in several herbal and traditional medicines, including diabetes. In the present study, we designed the methodology for the micropropagation of A. bracteosa from internodal segments. The highest shoot multiplication was achieved on Murashige and Skoog (MS) medium supplemented with 6-benzyl-amino-purine (BAP) (5.0 µM) + indole-3-acetic acid (IAA) (1.5 µM) + adenine sulphate (ADS) (15.0 µM), which produced the maximum number of 20.45 ± 0.12 shoots/explants with 6.43 ± 0.006 cm shoot length. Rooting in the microshoots was attained on half-strength MS medium containing indole-3-butyric acid (IBA) (1.5 µM), with the highest root number of 16.44 ± 0.015 roots/shoot, and root length of 2.25 ± 0.011 cm. To assess genetic fidelity, SCoT marker analysis was performed on nine randomly selected in vitro regenerated plantlets and the mother plant, all of which exhibited monomorphic banding patterns, confirming genetic stability. Scanning electron microscopy (SEM) reveals normal stomatal structure in the regenerated plants post-acclimatization, indicating successful physiological recovery. Furthermore, Gas Chromatography–Mass Spectrometry (GC-MS) analysis confirms the presence of major phytocompounds in both the in vitro regenerated plants and the mother plant, supporting the conservation of phytochemical integrity. Given the restricted distribution and overharvesting pressure on this species, the established protocol provides an efficient strategy for rapid, large-scale, and genetically stable propagation to support conservation and pharmaceutical utilization. Full article
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