Advances in Plant Molecular Biology and Gene Function

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: 10 February 2026 | Viewed by 227

Special Issue Editor


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Guest Editor
College of Life Sciences, Northeast Agricultural University, Harbin 150030, China
Interests: plant molecular biology; gene function; transcription factors; Dryopteris fragrans; fern

Special Issue Information

Dear Colleagues,

Research on plant molecular biology and gene function represents a cutting-edge frontier in modern life sciences, with its central aim being to unravel the fundamental principles of plant life processes through elucidating the molecular mechanisms of genes. The rapid advancement of high-throughput sequencing, gene editing, and multi-omics technologies has propelled this field from single-gene functional characterization to a new era of systematically investigating gene regulatory networks. Gene function studies not only provide crucial theoretical foundations for understanding fundamental biological processes such as plant growth and development, environmental adaptation, and secondary metabolism, but also pioneer novel approaches for crop genetic improvement, medicinal plant development, and synthetic biology applications.

Contemporary plant gene function research has evolved into a multidimensional, interdisciplinary technological framework. Genomic methodologies, including genome-wide association studies (GWASs) and quantitative trait locus (QTL) mapping, serve as powerful tools for identifying genes associated with important traits, while proteomics and metabolomics technologies reveal the molecular basis of gene function across different biological layers. Nevertheless, the field continues to face significant challenges that necessitate the development of more precise gene-editing tools, the establishment of more efficient functional validation systems, and the in-depth exploration of the dynamic characteristics of gene regulatory networks.

This Special Issue aims to compile the most recent advances in plant molecular biology and gene function research, with particular emphasis on the following: (1) the development of novel gene function analysis technologies, (2) the investigation of key gene regulatory mechanisms, and (3) applications in molecular breeding. We particularly encourage submissions employing innovative approaches such as integrated multi-omics analysis and AI-assisted prediction, as well as functional characterization of genes governing important agronomic traits and secondary metabolic regulation. By serving as an important platform for theoretical innovation and technological breakthroughs in plant gene function research, this Special Issue will contribute significantly to sustainable agricultural development and the efficient utilization of plant resources.

Prof. Dr. Ying Chang
Guest Editor

Manuscript Submission Information

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Keywords

  • plant molecular biology
  • gene function
  • gene editing
  • multi-omics technologies
  • gene regulation
  • molecular breeding
  • agronomic traits
  • secondary metabolism

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Published Papers (1 paper)

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Research

25 pages, 5845 KiB  
Article
Functional Identification and Transcriptional Activity Analysis of Dryopteris fragrans HMGR Gene
by Meng Sun, Qian Ma, Xueqi Wang, Jialiang Guo, Jiaxuan Wang, Dongrui Zhang, Kirill Tkachenko, Wenzhong Wang and Ying Chang
Plants 2025, 14(14), 2190; https://doi.org/10.3390/plants14142190 - 15 Jul 2025
Viewed by 175
Abstract
Dryopteris fragrans (L.) Schott synthesizes volatile sesquiterpenes through the mevalonate pathway (MVA), in which 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) serves as the key rate-limiting enzyme. Although HMGR plays a crucial role in terpenoid biosynthesis, its functional characteristics in D. fragrans and its involvement in stress [...] Read more.
Dryopteris fragrans (L.) Schott synthesizes volatile sesquiterpenes through the mevalonate pathway (MVA), in which 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) serves as the key rate-limiting enzyme. Although HMGR plays a crucial role in terpenoid biosynthesis, its functional characteristics in D. fragrans and its involvement in stress responses remain unclear. This study identified three HMGR genes (DfHMGR1/2/3) from the transcriptome data of D. fragrans. Bioinformatics analysis revealed that the encoded proteins are localized to the endoplasmic reticulum and share high sequence similarity with fern homologs. Under abiotic stress conditions, DfHMGRs exhibited differential expression patterns, with marked upregulation under salt and drought stress. To validate the functions of these genes, we generated transgenic Nicotiana tabacum L. plants overexpressing DfHMGRs. Compared with wild-type controls, the transgenic lines showed enhanced tolerance to drought and heat stress. Promoter analysis identified functional regulatory regions controlling DfHMGR expression, and co-expression network analysis predicted 21 potential transcriptional regulators. This study validates the function of D. fragrans HMGRs in a heterologous system and provides candidate genes for improving stress resistance in plants. Full article
(This article belongs to the Special Issue Advances in Plant Molecular Biology and Gene Function)
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