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Application of Chromatography in Food Analysis

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 7544

Special Issue Editor

Department of Food Science, Fu Jen Catholic University, New Taipei City 242062, Taiwan
Interests: food analysis; functional food development; value-added in food by-product; food safety

Special Issue Information

Dear Colleagues,

Chromatography has been developed for many years and is currently a powerful tool for analysis in the field of natural science, especially in food science. Since food is not a single composition system, the extraction efficiency will be affected by matrices that existed in different foods, and the outcomes will be seriously interfered with. Chromatography technology has the ability to separate a single component, and the influence of interference can be avoided to a considerable extent with effective pretreatment and powerful detectors. Therefore, chromatography can be widely applied in the development of health ingredients or monitoring of food safety.

This Special Issue is designed to gather scientific reviews or research articles related to food analysis by chromatography technology in any of the below-listed topics, but not limited to the list. Your topic can be analysis of natural toxins, functional components, animal ingested drugs, adulterants and additives from feed materials and environmental contaminants such as heavy metals, pesticides as well as toxins generated during food processing is also welcome. In addition, the identification of existing challenges and the possible future approaches will also be part of this Special Issue.

Prof. Dr. Tsai-Hua Kao
Guest Editor

Manuscript Submission Information

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Keywords

  • chromatography
  • food analysis
  • functional component
  • natural toxin
  • method development
  • food safety

Published Papers (4 papers)

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Research

19 pages, 5679 KiB  
Article
Qualitative and Quantitative Determination of Decoquinate in Chicken Tissues by Gas Chromatography Tandem Mass Spectrometry
by Shuyu Liu, Yayun Tang, Yang Lu, Yawen Guo, Kaizhou Xie, Fanxun Guan, Pengfei Gao, Yali Zhu, Yuhao Dong, Tao Zhang, Genxi Zhang, Guojun Dai and Xing Xie
Molecules 2023, 28(9), 3875; https://doi.org/10.3390/molecules28093875 - 04 May 2023
Viewed by 1082
Abstract
A novel precolumn derivatization–GC-MS/MS method was developed for the determination of decoquinate residues in chicken tissues (muscle, liver, and kidney). The samples were extracted and purified by liquid–liquid extraction combined with solid-phase extraction and derivatized with acetic anhydride and pyridine. The recovery rates [...] Read more.
A novel precolumn derivatization–GC-MS/MS method was developed for the determination of decoquinate residues in chicken tissues (muscle, liver, and kidney). The samples were extracted and purified by liquid–liquid extraction combined with solid-phase extraction and derivatized with acetic anhydride and pyridine. The recovery rates for decoquinate were 77.38~89.65%, and the intra-day and inter-day RSDs were 1.63~5.74% and 2.27~8.06%, respectively. The technique parameters meet the necessities for veterinary drug residue detection in China, the US, and the EU. Finally, the method was applied to analyze tissues of 60 chickens bought from a neighborhood supermarket, and solely one sample of chicken muscle contained 15.6 μg/kg decoquinate residue. Full article
(This article belongs to the Special Issue Application of Chromatography in Food Analysis)
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12 pages, 1538 KiB  
Article
Analysis of Furan and Its Derivatives in Food Matrices Using Solid Phase Extraction Coupled with Gas Chromatography-Tandem Mass Spectrometry
by Wen-Xuan Tsao, Bing-Huei Chen, Pinpin Lin, Shu-Han You and Tsai-Hua Kao
Molecules 2023, 28(4), 1639; https://doi.org/10.3390/molecules28041639 - 08 Feb 2023
Cited by 4 | Viewed by 1573
Abstract
The objective of this study was to develop a simultaneous analysis method of furan and its 10 derivatives in different food commodities. The results indicated that furan and its 10 derivatives could be separated within 9.5 min by using a HP-5MS column and [...] Read more.
The objective of this study was to develop a simultaneous analysis method of furan and its 10 derivatives in different food commodities. The results indicated that furan and its 10 derivatives could be separated within 9.5 min by using a HP-5MS column and gas chromatography–tandem mass spectrometry (GC-MS/MS) with multiple reaction monitoring mode for detection. Furthermore, this method could resolve several furan isomers, such as 2-methyl furan and 3-methyl furan, as well as 2,3-dimethyl furan and 2,5-dimethyl furan. The most optimal extraction conditions were: 5 g of the fruit or juice sample mixed with 5 mL of the saturated NaCl solution, separately, or 1 g of the canned oily fish sample mixed with 9 mL of the saturated NaCl solution, followed by the equilibration of each sample at 35 °C for 15 min, using a carboxen-polydimethylsiloxane SPME arrow to adsorb the analytes for 15 min at 35 °C for subsequent analysis by GC-MS/MS. For method validation of all the analytes in the different food matrices, the recovery was 76–117% and the limit of the quantitation was 0.003–0.675 ng/g, while the relative standard deviation (RSD%) of the intra-day variability range from 1–16%, and that of the inter-day variability was from 4–20%. The method validation data further demonstrated that a reliable method was established for the analysis of furan and its 10 derivatives in commercial foods. Full article
(This article belongs to the Special Issue Application of Chromatography in Food Analysis)
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17 pages, 3937 KiB  
Article
Untargeted LC-MS/MS-Based Multi-Informative Molecular Networking for Targeting the Antiproliferative Ingredients in Tetradium ruticarpum Fruit
by Chun-Han Su, Yu-Chieh Cheng, Yu-Chia Chang, Ting-Hsuan Kung, Yu-Li Chen, Kuei-Hung Lai, Hsi-Lung Hsieh, Chun-Yu Chen, Tsong-Long Hwang and Yu-Liang Yang
Molecules 2022, 27(14), 4462; https://doi.org/10.3390/molecules27144462 - 12 Jul 2022
Cited by 4 | Viewed by 2165
Abstract
The fruit of Tetradium ruticarpum (TR) is commonly used in Chinese herbal medicine and it has known antiproliferative and antitumor activities, which can serve as a good source of functional ingredients. Although some antiproliferative compounds are reported to be present in TR fruit, [...] Read more.
The fruit of Tetradium ruticarpum (TR) is commonly used in Chinese herbal medicine and it has known antiproliferative and antitumor activities, which can serve as a good source of functional ingredients. Although some antiproliferative compounds are reported to be present in TR fruit, most studies only focused on a limited range of metabolites. Therefore, in this study, the antiproliferative activity of different extracts of TR fruit was examined, and the potentially antiproliferative compounds were highlighted by applying an untargeted liquid chromatography–tandem mass spectrometry (LC-MS/MS)-based multi-informative molecular networking strategy. The results showed that among different extracts of TR fruit, the EtOAc fraction F2-3 possessed the most potent antiproliferative activity against HL-60, T24, and LX-2 human cell lines. Through computational tool-aided structure prediction and integrating various data (sample taxonomy, antiproliferative activity, and compound identity) into a molecular network, a total of 11 indole alkaloids and 47 types of quinolone alkaloids were successfully annotated and visualized into three targeted bioactive molecular families. Within these families, up to 25 types of quinolone alkaloids were found that were previously unreported in TR fruit. Four indole alkaloids and five types of quinolone alkaloids were targeted as potentially antiproliferative compounds in the EtOAc fraction F2-3, and three (evodiamine, dehydroevodiamine, and schinifoline) of these targeted alkaloids can serve as marker compounds of F2-3. Evodiamine was verified to be one of the major antiproliferative compounds, and its structural analogues discovered in the molecular network were found to be promising antitumor agents. These results exemplify the application of an LC-MS/MS-based multi-informative molecular networking strategy in the discovery and annotation of bioactive compounds from complex mixtures of potential functional food ingredients. Full article
(This article belongs to the Special Issue Application of Chromatography in Food Analysis)
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16 pages, 2046 KiB  
Article
A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS
by Bo-Yang Hsu, Chen-Te Jen, Baskaran Stephen Inbaraj and Bing-Huei Chen
Molecules 2022, 27(10), 3071; https://doi.org/10.3390/molecules27103071 - 11 May 2022
Cited by 5 | Viewed by 2221
Abstract
Ginseng (Panax quinquefolius), a popular herbal and nutritional supplement consumed worldwide, has been demonstrated to possess vital biological activities, which can be attributed to the presence of ginsenosides. However, the presence of ginsenosides in ginseng root residue, a by-product obtained during [...] Read more.
Ginseng (Panax quinquefolius), a popular herbal and nutritional supplement consumed worldwide, has been demonstrated to possess vital biological activities, which can be attributed to the presence of ginsenosides. However, the presence of ginsenosides in ginseng root residue, a by-product obtained during processing of ginseng beverage, remains unexplored. The objectives of this study were to develop a high-performance liquid chromatography-photodiode array detection-mass spectrometry (HPLC-DAD-ESI-MS) and an ultra-high-performance-liquid-chromatography-tandem mass spectrometry (UPLC-HRMS-MS/MS) method for the comparison of ginsenoside analysis in ginseng root residue. Results showed that by employing a Supelco Ascentis Express C18 column (150 × 4.6 mm ID, particle size 2.7 μm) and a gradient mobile phase of deionized water and acetonitrile with a flow rate at 1 mL/min and detection at 205 nm, a total of 10 ginsenosides, including internal standard saikosaponin A, were separated within 18 min and detected by HPLC-DAD-ESI-MS. Whereas with UPLC-HRMS-MS/MS, all the 10 ginsenosides were separated within six minutes by using an Acquity UPLC BEH C18 column (50 × 2.1 mm ID, particle size 1.7 μm, 130 Å) and a gradient mobile phase of ammonium acetate and acetonitrile with column temperature at 50 °C, flow rate at 0.4 mL/min and detection by selected reaction monitoring (SRM) mode. High accuracy and precision was shown, with limit of quantitation (LOQ) ranging from 0.2–1.9 μg/g for HPLC-DAD-ESI-MS and 0.269–6.640 ng/g for UPLC-HRMS-MS/MS. The contents of nine ginsenosides in the ginseng root residue ranged from <LOQ-26.39 mg/g by HPLC-DAD-ESI-MS and <LOQ-21.25 mg/g by UPLC-HRMS-MS/MS, with a total amount of 38.37 and 34.71 mg/g, respectively. Full article
(This article belongs to the Special Issue Application of Chromatography in Food Analysis)
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