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Extraction, Chromatography, and Mass Spectrometry Analysis of Food and Plant Biomolecules

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 31 July 2026 | Viewed by 842

Special Issue Editors


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Guest Editor
CQM—Centro de Químcia da Madeira, Universidade da Madeira, 9000 Funchal, Portugal
Interests: food composition in bioactive compounds; food quality and degradation; markers for food origin and authenticity; microextraction; chromatographic analysis; foodomics
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Departamento de Tecnología Química y Ambiental, Universidad Rey Juan Carlos, Calle Tulipán, 28933 Móstoles, Madrid, Spain
Interests: bioactive compounds; analytical methods; mass spectrometry; miniaturization; natural toxins; sample preparation; liquid chromatography; contaminants; microextraction; novel sorbents
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In chemistry, the processes of sample extraction and chromatographic analysis are crucial for isolating and purifying chemical substances from complex mixtures. These techniques are extensively employed in various sectors, such as pharmaceutical production, biotechnology, environmental evaluations, and food science research. Extraction is the process of selectively removing certain components from a mixture by using a suitable solvent system and chromatography operates by separating the elements of a mixture based on their distinct physical and chemical properties.

This Special Issue follows a previous successful Special Issue, concentrating on cutting-edge technologies and applications in chromatography and extraction methods for chemical analysis. It includes both original research papers and review articles exploring the development, implementation, and refinement of chromatographic and extraction techniques across various chemical applications. The Special Issue covers advancements in chromatography and extraction theory, equipment, sample preparation, and data interpretation, as well as their use in food, medicinal, and environmental analyses. Furthermore, it welcomes contributions on novel approaches to method development and validation, innovative strategies for detecting trace contaminants, and other relevant topics in the field. This compilation serves as an essential resource for readers seeking to further the progress of chromatography and extraction techniques in chemical applications.

Dr. Jorge Pereira
Dr. Natalia Casado Navas
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • liquid chromatography
  • gas chromatography
  • sample preparation
  • extraction
  • supercritical fluid extraction
  • solid-phase extraction
  • liquid–liquid extraction

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Published Papers (1 paper)

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Research

18 pages, 875 KB  
Article
Ultrasound-Assisted Extraction: Unlocking the Antibacterial Potential of Coptis chinensis Franch. Against ESBL-Producing Enterobacterales
by Ching Ching Hui, Fred Wang Fat Lee, Wesley Chin Ho Lung, Kai Chung Fan, Ivan Tak Fai Wong, Gilman Kit Hang Siu, Yeuk Lung Chow, Ping Lung Chan, Siu-Mui Ng, Ling Shi, Sai Wang Seto, Franklin Wang Ngai Chow and Emily Wan Ting Tam
Molecules 2025, 30(22), 4331; https://doi.org/10.3390/molecules30224331 - 7 Nov 2025
Viewed by 643
Abstract
The global rise of multidrug-resistant Extended Spectrum β-Lactamase Producing-Enterobacterales (ESBL-PE) poses a critical threat to public health, driving the urgent need for alternative therapeutic approaches. This study evaluates the antimicrobial properties of 20 Chinese medicinal herbs against 14 ESBL-PE strains from seven [...] Read more.
The global rise of multidrug-resistant Extended Spectrum β-Lactamase Producing-Enterobacterales (ESBL-PE) poses a critical threat to public health, driving the urgent need for alternative therapeutic approaches. This study evaluates the antimicrobial properties of 20 Chinese medicinal herbs against 14 ESBL-PE strains from seven bacterial species, utilizing three extraction methods: traditional water decoction, 80% ethanol maceration, and 50% ethanol with ultrasound-assisted extraction (UAE). Among the herbs tested, Coptis chinensis Franch. demonstrated the most potent anti-ESBL-PE activity, effectively inhibiting multiple strains, including Escherichia coli, Klebsiella pneumoniae, Hafnia alvei, Citrobacter freundii and Proteus hauseri. C. chinensis extracts obtained via UAE exhibited superior antibacterial activity to the other two extraction methods, attributed to enhanced extraction efficiency and improved bioactive compound yields. Specifically, UAE increased the extraction yield of alkaloids in C. chinensis by 80.9%, compared to the ethanol maceration method, and the increase in berberine, the key antimicrobial compound, was 75.4%. Berberine demonstrated significant antibacterial effects against ESBL-PE strains, while other phytochemicals in C. chinensis extracts have an additional effect with berberine, further amplifying the overall antimicrobial activity. These findings highlight that the UAE is a promising method for enhancing the therapeutic potential of C. chinensis and other Chinese medicinal herbs against multidrug-resistant bacteria. Full article
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