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Bioactive Molecules in Foods: From Sources to Functional Applications

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Food Chemistry".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 2505

Special Issue Editors


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Guest Editor
Department of Biochemistry and Food Chemistry, The University of Life Sciences, Skromna 8 St., 20-704 Lublin, Poland
Interests: food compounds; peptides; antioxidant properties; food technology, enzyme inhibitory; metabolic syndrome
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Environmental Microbiology, The University of Life Sciences, Leszczyńskiego 7 St., 20-069 Lublin, Poland
Interests: environmental microbiology; environmental biotechnology; bioactive compounds; antimicrobial activity of natural compound
Special Issues, Collections and Topics in MDPI journals

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Guest Editor Assistant
Department of Biochemistry and Food Chemistry; University of Life Sciences in Lublin, Lublin, Poland
Interests: bioactive components; food chemistry; functional food; encapsulation; spray-drying
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Food is a source of ingredients that provides the body with energy and affect its proper functioning. Many play a key role in regulating biochemical processes and exhibit many bioactive properties such as antioxidant, antidiabetic, antiobesity, or antimicrobial. Molecules from food may prevent diseases, including diseases of civilization such as hypertension, depression, obesity, or cancer. Moreover, food compounds may be used in food technology as ingredients that improve the quality and durability of food.

Therefore, it is important to search for new sources of bioactive food ingredients and the possibility of their use in food production, diet, or dietary supplements. Bioactive food compounds may be obtained from plants, animals, or microorganisms, but nowadays there is a search for new or alternative sources of food with the “zero waste” principle.

For this Special Issue on “Bioactive Molecules in Foods: From Sources to Functional Applications”, we welcome papers that provide information about the origin of food ingredients and their biological activity that can be used in nutrition or food products to improve the proper organism functioning.

Dr. Anna Jakubczyk
Dr. Kamila Rybczyńska-Tkaczyk
Guest Editors

Dr. Katarzyna Lisiecka
Guest Editor Assistant

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • food compounds
  • biological activity
  • nutrition
  • health
  • food products
  • diet supplementary

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Published Papers (2 papers)

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Research

26 pages, 7715 KiB  
Article
Harnessing Nature’s Chemistry: Deciphering Olive Oil Phenolics for the Control of Invasive Breast Carcinoma
by Nehal A. Ahmed, Abu Bakar Siddique, Afsana Tajmim, Judy Ann King and Khalid A. El Sayed
Molecules 2025, 30(15), 3157; https://doi.org/10.3390/molecules30153157 - 28 Jul 2025
Viewed by 499
Abstract
Breast cancer (BC) is the most common malignancy and the second-leading cause of cancer-related mortalities in women. Epidemiological studies suggested the reduced BC incidence in Mediterranean populations due to the daily consumption of diets rich in extra-virgin olive oil (EVOO). EVOO secoiridoid phenolics [...] Read more.
Breast cancer (BC) is the most common malignancy and the second-leading cause of cancer-related mortalities in women. Epidemiological studies suggested the reduced BC incidence in Mediterranean populations due to the daily consumption of diets rich in extra-virgin olive oil (EVOO). EVOO secoiridoid phenolics are widely known for their positive outcomes on multiple cancers, including BC. The current study investigates the suppressive effects of individual and combined EVOO phenolics for BC progression and motility. Screening of a small library of EVOO phenolics at a single dose of 10 µM against the viability of the BC cell lines ZR-75-1 (luminal A) and MDA-MB-231 (triple negative BC, TNBC) identified oleocanthal (OC) and ligstroside aglycone (LA) as the most active hits. Screening of EVOO phenolics for BC cells migration inhibition identified OC, LA, and the EVOO lignans acetoxypinoresinol and pinoresinol as the most active hits. Combination studies of different olive phenolics showed that OC combined with LA had the best synergistic inhibitory effects against the TNBC MDA-MB-231 cells migration. A combination of 5 µM of each of OC and LA potently suppressed the migration and invasion of the MDA-MB-231 cells versus LA and OC individual therapies and vehicle control (VC). Animal studies using the ZR-75-1 BC cells orthotopic xenografting model in female nude mice showed significant tumor progression suppression by the combined OC-LA, 5 mg/kg each, ip, 3X/week treatments compared to individual LA and OC treatments and VC. The BC suppressive effects of the OC-LA combination were associated with the modulation of SMYD2–EZH2–STAT3 signaling pathway. A metastasis–clonogenicity animal study model using female nude mice subjected to tail vein injection of MDA-MB-231-Luc TNBC cells also revealed the effective synergy of the combined OC-LA, 5 mg/kg each, compared to their individual therapies and VC. Thus, EVOO cultivars rich in OC with optimal LA content can be useful nutraceuticals for invasive hormone-dependent BC and TNBC progression and metastasis. Full article
(This article belongs to the Special Issue Bioactive Molecules in Foods: From Sources to Functional Applications)
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21 pages, 3141 KiB  
Article
Biorefining Brazilian Green Propolis: An Eco-Friendly Approach Based on a Sequential High-Pressure Extraction for Recovering High-Added-Value Compounds
by Guilherme Dallarmi Sorita, Wilson Daniel Caicedo Chacon, Monique Martins Strieder, Camilo Rodriguez-García, Alcilene Monteiro Fritz, Silvani Verruck, Germán Ayala Valencia and José A. Mendiola
Molecules 2025, 30(1), 189; https://doi.org/10.3390/molecules30010189 - 6 Jan 2025
Cited by 3 | Viewed by 1673
Abstract
Propolis is a valuable natural resource for extracting various beneficial compounds. This study explores a sustainable extraction approach for Brazilian green propolis. First, supercritical fluid extraction (SFE) process parameters were optimized (co-solvent: 21.11% v/v CPME, and temperature: 60 °C) to maximize [...] Read more.
Propolis is a valuable natural resource for extracting various beneficial compounds. This study explores a sustainable extraction approach for Brazilian green propolis. First, supercritical fluid extraction (SFE) process parameters were optimized (co-solvent: 21.11% v/v CPME, and temperature: 60 °C) to maximize yield, total phenolic content (TPC), antioxidant capacity, and LOX (lipoxygenase) inhibitory activity. GC–MS analysis identified 40 metabolites in SFE extracts, including fatty acids, terpenoids, phenolics, and sterols. After selecting the optimum SFE process parameters, a sequential high-pressure extraction (HPE) approach was developed, comprising SFE, pressurized liquid extraction (PLE) with EtOH/H2O, and subcritical water extraction (SWE). This process was compared to a similar sequential extraction using low-pressure extractions (LPE) with a Soxhlet extractor. The HPE process achieved a significantly higher overall yield (80.86%) than LPE (71.43%). SFE showed higher selectivity, resulting in a lower carbohydrate content in the non-polar fraction, and PLE extracted nearly twice the protein amount of LPE–2. Despite the HPE selectivity, LPE extracts exhibited better acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and LOX inhibition, demonstrating that the neuroprotective and anti-inflammatory activity of the extracts may be associated with a symbiosis of a set of compounds. Finally, a comprehensive greenness assessment revealed that the HPE process proved more sustainable and aligned with green chemistry principles than the LPE method. Full article
(This article belongs to the Special Issue Bioactive Molecules in Foods: From Sources to Functional Applications)
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