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Modern Sample Preparation Approaches for Separation Science, 2nd Edition

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 30 June 2026 | Viewed by 1507

Special Issue Editor

1. Laboratório de Ciências Forenses e Psicológicas Egas Moniz, Egas Moniz Center for Interdisciplinary Research, Egas Moniz School of Health & Science, Almada, Portugal
2. Centro de Química Estrutural, Institute of Molecular Sciences, Faculdade de Ciências, Universidade de Lisboa, Lisboa, Portugal
Interests: new psychoactive substances; analytical chemistry; sample preparation; microextraction techniques; chromatography and hyphenated techniques (GC-MS and LC-MS); environmental and biological analysis; forensic analysis
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Special Issue Information

Dear Colleagues,

Following the success of the first Special Issue on Modern Sample Preparation Approaches for Separation Science, it is our pleasure to announce this second edition.

Sample preparation is an essential step in most analytical methods for environmental and biomedical analysis since target analytes are often not detectable in their in situ forms, or because the results are distorted by interfering species. These preparation techniques often involve elaborate and time-consuming procedures that can take up to 80% of the total analysis time.

In the last decade, modern sample preparation techniques have aimed to comply with green analytical chemistry principles, leading to simplification, miniaturization, easy manipulation of the analytical devices, low costs, the strong reduction or absence of organic toxic solvents, and low sample-volume requirements.

With this Special Issue, we aim to categorize state-of-the-art sample preparation techniques. We are particularly interested in research that works towards new sample enrichment approaches for separation science, e.g., chromatography, electrophoresis, and hyphenated techniques.

Potential topics include, but are not limited to, the following:

  • New sample preparation concepts;
  • Selective enrichment methods;
  • Sorption-based materials, nanomaterials, or nanostructured materials for sample preparation;
  • Virtual sample preparation strategies.

Full papers, short communications, and review articles are welcome.

Dr. Nuno Neng
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • sample preparation
  • enrichment techniques
  • sorption-based methods
  • virtual sample preparation
  • microextraction techniques
  • new analytical strategies
  • separation techniques

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Related Special Issue

Published Papers (2 papers)

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Research

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15 pages, 1609 KB  
Article
Sorbent Strip Microextraction as a Practical Tool for Drug Screening: Application to Opioids and Local Anesthetics in Human Urine
by Marisa H. Maria, Thomas Berg and Nuno R. Neng
Molecules 2026, 31(4), 605; https://doi.org/10.3390/molecules31040605 - 9 Feb 2026
Viewed by 567
Abstract
The present contribution proposes a new design for adsorptive microextraction devices that promote a user-friendly and greener analytical approach. Novel Sorbent Strip Microextraction (SSμE) devices were made using a flexible adhesive film coated with convenient sorbents. Comparing the previous adsorptive microextraction devices, i.e., [...] Read more.
The present contribution proposes a new design for adsorptive microextraction devices that promote a user-friendly and greener analytical approach. Novel Sorbent Strip Microextraction (SSμE) devices were made using a flexible adhesive film coated with convenient sorbents. Comparing the previous adsorptive microextraction devices, i.e., bar adsorptive microextraction and multi-sphere adsorptive microextraction, the main advantage of the sorbent strip device is its simple design and reduced device preparation time and waste. To demonstrate its applicability, three opioids (buprenorphine, tapentadol, and tramadol) and two local anesthetics (articaine and bupivacaine) were used as model compounds in urine matrices, followed by high-performance liquid chromatography with diode array detector (HPLC-DAD) analysis. Key parameters such as sorbent type, desorption conditions, and microextraction variables were systematically optimized by experimental designs. Under the final conditions, the method achieved recoveries ranging from 78% to 108%, trueness within ±7% and precision expressed by relative standard deviation below 13%. The technique demonstrated good linearity (r2 ≥ 0.9922) across dynamic ranges of 5–500 ng/mL for local anesthetics and 50–5000 ng/mL for opioids. The validated SSμE/HPLC-DAD methodology was successfully applied to real urine samples, confirming its high precision and accuracy. The proposed microextraction technique offers a practical, eco-friendly, and effective alternative for routine drug screening in complex biological matrices and presents significant advantages over traditional and other microextraction-based methods. Full article
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Review

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33 pages, 2342 KB  
Review
In-Tube Solid Phase Microextraction: Basic Concepts and Recent Applications in Food Matrices
by Maria Flávia Assunção Magalhães, Rafael Oliveira Martins, Josicleia Oliveira Costa, Jussara da Silva Alves and Fernando Mauro Lanças
Molecules 2026, 31(4), 730; https://doi.org/10.3390/molecules31040730 - 20 Feb 2026
Viewed by 512
Abstract
In-tube solid-phase microextraction (IT-SPME) is an advanced microextraction technique in which a sample solution flows through a capillary containing an internal stationary phase, enabling efficient extraction and preconcentration of target analytes. The online coupling to liquid chromatography is a key advantage of this [...] Read more.
In-tube solid-phase microextraction (IT-SPME) is an advanced microextraction technique in which a sample solution flows through a capillary containing an internal stationary phase, enabling efficient extraction and preconcentration of target analytes. The online coupling to liquid chromatography is a key advantage of this technique, enabling full automation and high analytical throughput, both of which are significant for food analysis. Recent advances have focused on developing novel sorbent materials that respond to external stimuli (e.g., magnetic, electrical, or thermal) and on integrating them into emerging chromatographic platforms. Moreover, key operational parameters, including sample volume, pH, phase thickness, and the capillary’s dimensions (length and inner diameter), must be optimized to achieve enhanced selectivity, speed, and sensitivity. Despite this, the literature still lacks updated reviews of SPME concepts and their innovations for versatile applications in food matrices. Hence, this review outlines the fundamental principles of IT-SPME while highlighting key parameters that affect analytical performance. Finally, we provide a literature review of SPME applications in food analysis over the past 6 years, while exploring current trends and future directions for SPME development and enhanced applications in food science. Full article
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