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Extraction and Analysis of New Bioactive Compounds Derived from Natural Products, 3rd Edition

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: 30 June 2026 | Viewed by 746

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Department of Analytical Chemistry and Physical Chemistry, Faculty of Chemistry, University of Bucharest, 030018 Bucharest, Romania
Interests: separation sciences; HPLC; HPLC-MS; GC-MS; spectrometry; NMR; IR; pharmaceutical analysis; electrochemistry
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Special Issue Information

Dear Colleagues,

Natural products have been used for a very long time as food supplements and drugs. To date, according to the WHO, more than 80% of countries have developed treatments based on traditional medicine, which employs phytochemical compounds, and almost 70000 species have been tested for their biological properties. These studies reveal the huge advantage of using compounds derived from natural products, relying on their safety and broad efficiency to treat diseases. Thus, they present real competition to synthetic molecules.

Under these circumstances, the assessment of new compounds from natural products derived from terrestrial or marine plants and animals represents a continuous area of interest. Thus, new extraction and analytical methodologies that fit the requirement of green chemistry should be encouraged in order to obtain not only valuable extracts but also, most importantly, standardized ones. Despite the rewarding utilization of these natural products, the interest in them has decreased in recent years; therefore, it is high time that this field is revitalized.

Thus, this Special Issue will be devoted to the extraction and analysis of new bioactive compounds derived from natural products.

Dr. Irinel Adriana Badea
Dr. Rodica Olar
Guest Editors

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Keywords

  • phytochemicals
  • natural products
  • traditional medicine
  • liquid–liquid extraction
  • hydro distillation and steam distillation
  • solid-phase extraction
  • Soxhlet extraction
  • pressurized liquid extraction
  • supercritical fluid extraction
  • ultrasound-assisted extraction
  • microwave-assisted extraction
  • enzyme-assisted extraction
  • pulsed electric field extraction
  • HPLC
  • HPLC-MS
  • GC-MS
  • NMR
  • IR
  • in vitro assay
  • in vivo assay
  • antioxidant
  • anti-inflammatory
  • antimicrobial
  • antitumoral activity

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Published Papers (1 paper)

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Research

28 pages, 2628 KB  
Article
Scalable Jet-O-Mized Eggshell Membrane Processing for Bioactives Promoting Gut Health
by Manar Younes, Tamer A. E. Ahmed, Riadh Hammami and Maxwell T. Hincke
Molecules 2026, 31(7), 1217; https://doi.org/10.3390/molecules31071217 - 7 Apr 2026
Viewed by 475
Abstract
This study introduces a novel, simplified, and scalable two-step process for generating bioactive eggshell membrane (ESM) formulations by combining jet-O-mizer ultra-fine milling of ESM (yielding JEM biomaterial) with KOH-mediated hydrolysis, achieving ~50% solubilization of proteins and peptides and enabling the first evaluation of [...] Read more.
This study introduces a novel, simplified, and scalable two-step process for generating bioactive eggshell membrane (ESM) formulations by combining jet-O-mizer ultra-fine milling of ESM (yielding JEM biomaterial) with KOH-mediated hydrolysis, achieving ~50% solubilization of proteins and peptides and enabling the first evaluation of ESM-derived bioactives for gut health applications. The soluble protein fraction (SJ) was separated from the whole hydrolysate (WJ), and subjected to simulated gastrointestinal digestion to assess stability and bioavailability. The antioxidant capacities of the JEM-derived material showed a significant 15-fold increase compared to soluble non-hydrolyzed JEM (NJEM). SJ inhibited E. coli bacterial growth by 50% within 24 h, compared to the untreated bacterial culture. The formulations demonstrated superior anti-inflammatory properties with lipopolysaccharide (LPS)-induced RAW macrophages, resulting in a 80% reduction in NO production compared to untreated cells. Proteomics analysis of SJ revealed key anti-inflammatory (YBX1, YWHAE) and antimicrobial (OCX36, OC-17, TENP, and histones) effectors whose coordinated activities could modulate gut microbial composition. The permeability of the intestinal barrier model Caco-2 monolayer was not significantly affected by treatment with any JEM-derived formulation, thereby predicting maintenance of intestinal integrity. This study provides safe, novel ESM derivatives with high bioavailability and multifunctional bioactivities, including antibacterial, antioxidant, and anti-inflammatory effects, positioning them as promising candidates for dietary supplements to promote gut health. Full article
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