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Micromachines
Special Issues
Advances and Applications of Electrochemical Immunosensors
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Advances and Applications of Electrochemical Immunosensors

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "C：Chemistry".

Deadline for manuscript submissions: closed (15 January 2021) | Viewed by 3099

Special Issue Editor

Dr. Ajay Kumar Yagati

E-Mail Website
Guest Editor
Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany
Interests: biosensors; bioimpedance; nanomaterials and nanotechnology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear colleagues,

The combination of specific antibody–antigen recognition with the high sensitivity and selectivity of electrochemical immunosensors has been the focus of tremendous interest and there has been significant progress over the past decade in early disease diagnostics in clinical applications, environmental monitoring, and food industry applications. Electrochemical immunosensors have many advantages, such as high sensitivity, operational simplicity, low instrumentational cost, the possibility for miniaturization, and the ability to detect trace amounts of analytical targets of biological significance on a scale ranging from nano to macro level and are also promising for point-of-care-testing. Electrochemical transducing methods such as voltammetric, potentiometric, conductometric, or impedimetric have been utilized in different applications because of their excellent properties, such as low-cost, sensitivity, and simplicity. A vast majority of recent research on electrochemical immunosensors has focused on various detection methodologies and incorporation of nanomaterials in design to achieve high sensitivity in terms of electrochemical change of signal transduction. This book focuses on recent developments of electrochemical immunosensors, with an emphasis on conventional methodologies of voltammetry, amperometry, electrochemical impedance, and innovative designs for transducer surface modifications and analytical platforms.

Dr. Ajay Kumar Yagati
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Immunosensors
  • immunoassay
  • antibody
  • aptamers
  • nanomaterials
  • clinical analysis
  • point-of-care- testing

Published Papers (1 paper)

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Research

13 pages, 4325 KiB  
Article
A Probeless Capacitive Biosensor for Direct Detection of Amyloid Beta 1-42 in Human Serum Based on an Interdigitated Chain-Shaped Electrode
by Hien T. Ngoc Le, Jinsoo Park and Sungbo Cho
Micromachines 2020, 11(9), 791; https://doi.org/10.3390/mi11090791 - 21 Aug 2020
Cited by 22 | Viewed by 2712
Abstract
Amyloid beta (aβ) 1-42, a peptide that is 1-42 amino acids long, is a major component of senile plaques in the brains of patients with Alzheimer’s disease. Aβ detection has become an essential antecedence to predict the declining mental abilities of patients. In [...] Read more.
Amyloid beta (aβ) 1-42, a peptide that is 1-42 amino acids long, is a major component of senile plaques in the brains of patients with Alzheimer’s disease. Aβ detection has become an essential antecedence to predict the declining mental abilities of patients. In this paper, a probeless capacitive biosensor for the non-Faradaic detection of aβ 1-42 peptide was developed by immobilizing a specific anti-aβ antibody onto a self-assembled monolayer functionalized interdigitated chain-shaped electrode (anti-aβ/SAM/ICE). The novelty and difference of this article from previous studies is the direct detection of aβ peptide with no redox probe ((Fe(CN)6)3−/4−), which can avoid the denaturation of the protein caused by the metallization (binding of aβ to metal ion Fe which is presented in the redox couple). The direct detection of aβ with no redox probe is performed by non-Faradaic capacitive measurement, which is greatly different from the Faradaic measurement of the charge transfer resistance of the redox probe. The detection of various aβ 1-42 peptide concentrations in human serum (HS) was performed by measuring the relative change in electrode interfacial capacitance due to the specific antibody-aβ binding. Capacitance change in the anti-aβ/SAM/ICE biosensor showed a linear detection range between 10 pg mL−1 and 104 pg mL−1, and a detection limit of 7.5 pg mL−1 in HS, which was much lower than the limit of detection for CSF aβ 1-42 (~500 pg mL−1) and other biosensors. The small dissociation constant Kd of the antibody-antigen interaction was also found to be 0.016 nM in HS, indicating the high binding affinity of the anti-aβ/SAM/ICE biosensor in the recognizing of aβ 1-42. Thus, the developed sensor can be used for label-free and direct measurement of aβ 1-42 peptide and for point-of-care diagnosis of Alzheimer’s disease without redox probe. Full article
(This article belongs to the Special Issue Advances and Applications of Electrochemical Immunosensors)
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