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Clinical Advances in HPV-Related Disease

A Special Issue of Journal of Clinical Medicine (ISSN 2077-0383) belonging to the section "Obstetrics & Gynecology".

Deadline for manuscript submissions: 20 November 2026 | Viewed by 983

Editors


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Guest Editor
1. Dominik Pruski Gynecology Specialised Practise, 60-408 Poznań, Poland
2. Department of Obstetrics and Gynecology, District Public Hospital in Poznan, 60-479 Poznań, Poland
Interests: gynecological cancers; HPV; HPV vaccine; cervical cancer; precancerous lesions diagnostics

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Guest Editor Assistant
Department of Obstetrics and Gynecology, District Public Hospital in Poznan, 60-479 Poznań, Poland
Interests: HPV; SIL; cervical cancer screening; vulvar cancer; HPV vaccination; VIN; HPV-related diseases; ovarian cancer; cervical cancer

Special Issue Information

Dear Colleagues,

HPV disease is a significant health problem worldwide. In developed countries, where cervical cancer incidence and mortality rates have decreased significantly, precancerous changes in the cervix, vulva, and vagina remain a major problem. Medicine is constantly searching for newer solutions—diagnostic tools that will most precisely identify patients with the highest risk of high-grade precancerous cervical lesions and, as a consequence, cervical cancer. The discoveries of the last 50 years have significantly influenced the prevention and treatment of cervical cancer. The first milestone was the detection of HPV by Professor Harald zur Hausen, followed by the creation of prophylactic vaccines against HPV and the introduction of HPV-dependent screening for cervical cancer. In the field of oncological gynecology, the biggest challenge in the coming decades will be the diagnosis and treatment of SIL-type lesions—involving the cervix, vagina, vulva, and anus. Especially important is the diagnosis and treatment of persistent infection with highly oncogenic HPV types. At the moment, several molecular tests have been implemented to detect the genetic material of the virus embedded in the genome of the host cell. As doctors, we must also be prepared for the diagnosis and treatment of independent HPV diseases, where liquid LBC or even conventional cytology may undergo a renaissance and may be the tool of choice when there is a lack of replication in tissues.

Dr. Dominik Pruski
Guest Editor

Dr. Sonja Millert-Kalińska
Guest Editor Assistant

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Keywords

  • gynecological cancers
  • HPV
  • HPV vaccine
  • cervical cancer
  • precancerous lesions diagnostics

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Published Papers (1 paper)

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Research

12 pages, 4109 KB  
Article
Detection of HPV DNA in Cervical Intraepithelial Neoplasia Using In Situ Hybridization
by Marcin Przybylski, Sonja Millert-Kalińska, Dominik Pruski, Mateusz de Mezer, Monika Krzyżaniak, Robert Jach, Jakub Żurawski and Paweł Kurzawa
J. Clin. Med. 2026, 15(10), 3974; https://doi.org/10.3390/jcm15103974 - 21 May 2026
Viewed by 614
Abstract
Background: Human papillomavirus (HPV)-related diseases remain a major global health problem, with cervical intraepithelial neoplasia (CIN) representing a key precursor to cervical cancer. Identification of high-risk HPV genotypes is essential for early diagnosis and appropriate management. This study aimed to evaluate the [...] Read more.
Background: Human papillomavirus (HPV)-related diseases remain a major global health problem, with cervical intraepithelial neoplasia (CIN) representing a key precursor to cervical cancer. Identification of high-risk HPV genotypes is essential for early diagnosis and appropriate management. This study aimed to evaluate the usefulness of in situ hybridization (ISH) for detecting HPV DNA in formalin-fixed, paraffin-embedded (FFPE) cervical tissue and to compare automated signal detection with manual histopathological assessment. Methods: This prospective, non-randomized study included 83 women undergoing diagnostic procedures for abnormal cytology or confirmed CIN between 2022 and 2023. Tissue specimens obtained during a loop electrosurgical excision procedure (LEEP) were examined using two ISH probes: ISH II for low-risk HPV types 6 and 11, and ISH III for high-risk HPV genotypes. Staining patterns and distributions were evaluated and correlated with molecular HPV testing and histopathological outcomes. Results: ISH II distribution was significantly associated with the presence of HPV type 6 or 11 (p < 0.001), although stain structure itself was not. ISH III stain structure was significantly associated with high-risk HPV genotypes (p = 0.020). A positive ISH II result predicted low-risk HPV infection with a sensitivity of 62.5% and specificity of 64.0%, while ISH III predicted high-risk HPV infection with a sensitivity of 86.36% but lower specificity (23.53%). Overall diagnostic accuracy was 63.86% for ISH II and 73.49% for ISH III. Conclusions: ISH proved to be a reproducible method for detecting HPV in archived cervical tissue, enabling assessment even years after specimen collection. Although PCR-based methods remain more widely used due to higher sensitivity and less invasive sampling, ISH provides valuable morphological context and may serve as a complementary diagnostic tool, particularly when only archival tissue is available. Full article
(This article belongs to the Special Issue Clinical Advances in HPV-Related Disease)
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