A green synthesis route for the production of silver nanoparticles using methanol extract from
Solanum xanthocarpum berry (SXE) is reported in the present investigation. Silver nanoparticles (AgNps), having a surface plasmon resonance (SPR) band centered at 406 nm, were synthesized by reacting SXE
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A green synthesis route for the production of silver nanoparticles using methanol extract from
Solanum xanthocarpum berry (SXE) is reported in the present investigation. Silver nanoparticles (AgNps), having a surface plasmon resonance (SPR) band centered at 406 nm, were synthesized by reacting SXE (as capping as well as reducing agent) with AgNO
3 during a 25 min process at 45 °C. The synthesized AgNps were characterized using UV–Visible spectrophotometry, powdered X-ray diffraction, and transmission electron microscopy (TEM). The results showed that the time of reaction, temperature and volume ratio of SXE to AgNO
3 could accelerate the reduction rate of Ag
+ and affect the AgNps size and shape. The nanoparticles were found to be about 10 nm in size, mono-dispersed in nature, and spherical in shape.
In vitro anti-Helicobacter pylori activity of synthesized AgNps
was tested against 34 clinical isolates and two reference strains of
Helicobacter pylori by the agar dilution method and compared with AgNO
3 and four standard drugs, namely amoxicillin (AMX), clarithromycin (CLA), metronidazole (MNZ) and tetracycline (TET), being used in anti-
H. pylori therapy. Typical AgNps sample (S1) effectively inhibited the growth of
H. pylori, indicating a stronger anti-
H. pylori activity than that of AgNO
3 or MNZ, being almost equally potent to TET and less potent than AMX and CLA. AgNps under study were found to be equally efficient against the antibiotic-resistant and antibiotic-susceptible strains of
H. pylori. Besides, in the
H. pylori urease inhibitory assay, S1 also exhibited a significant inhibition. Lineweaver-Burk plots revealed that the mechanism of inhibition was noncompetitive.
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