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Molecular Perspectives in Tissue Engineering and Regenerative Medicine

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: closed (20 May 2026) | Viewed by 1386

Special Issue Editor

Special Issue Information

Dear Colleagues,

This Special Issue is dedicated to exploring the molecular foundations of tissue engineering and regenerative medicine. It aims to highlight cutting-edge research that investigates the molecular mechanisms driving tissue formation, repair, and integration, with a focus on translating molecular insights into innovative therapeutic strategies. We particularly seek to emphasize emerging strategies that manipulate molecular cues to enhance stem cell behaviour, modulate immune responses, and guide tissue regeneration. By examining these processes at the molecular level, we hope to bridge the gap between fundamental science and translational applications, fostering the development of next-generation regenerative therapies.

We invite researchers from interdisciplinary backgrounds to contribute to this Special Issue and help shape the future of regenerative medicine through molecular innovation. We welcome original research articles, reviews, and short communications that address, but are not limited to, the following topics:

  • The molecular regulation of stem cell differentiation and behaviour;
  • Growth factors, cytokines, and signalling pathways in tissue regeneration;
  • Gene editing and molecular engineering approaches for regenerative applications;
  • Extracellular matrix remodelling and cell–matrix interactions;
  • Molecular crosstalk in engineered tissue environments;
  • Immunomodulation at the molecular level in regenerative therapies;
  • Molecular diagnostics and biomarkers for tissue regeneration outcomes;
  • Omics-based approaches (genomics, proteomics, and metabolomics) in tissue engineering.

Dr. Sorina Dinescu
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

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Keywords

  • 3D scaffolds
  • personalized regenerative therapies
  • tissue engineering
  • stem cells
  • bone regeneration
  • regenerative biomarkers
  • cell–scaffold interactions
  • nanomaterials

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Published Papers (1 paper)

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Research

20 pages, 13526 KB  
Article
PTEN Inhibition Suppresses Differentiation in Periodontal Ligament Stem Cells
by Suphalak Phothichailert, Nunthawan Nowwarote, Chatvadee Kornsuthisopon, Supreda Suphanantachat Srithanyarat, Vorapat Trachoo, Worachat Namangkalakul, Hiroshi Egusa and Thanaphum Osathanon
Int. J. Mol. Sci. 2026, 27(4), 2069; https://doi.org/10.3390/ijms27042069 - 23 Feb 2026
Viewed by 856
Abstract
Phosphatase and Tensin Homolog (PTEN) functions in numerous biological processes, encompassing cell proliferation, growth, self-renewal, and differentiation. This study examined the modulatory function of the PTEN inhibitor in periodontal ligament stem cells (PDLSCs). PDLSCs were treated with VO-OHpic at a concentration range from [...] Read more.
Phosphatase and Tensin Homolog (PTEN) functions in numerous biological processes, encompassing cell proliferation, growth, self-renewal, and differentiation. This study examined the modulatory function of the PTEN inhibitor in periodontal ligament stem cells (PDLSCs). PDLSCs were treated with VO-OHpic at a concentration range from 0.625 to 5 μM. MTT assay and Coomassie Blue staining were conducted to determine cell viability and colony-forming unit ability, respectively. The scratch assay was employed to examine cell migration. Mineral deposition and intracellular lipid accumulation were assessed. The qRT-PCR and immunofluorescence were used to evaluate mRNA and protein expression, respectively. RNA sequencing was employed for transcriptomic analysis. VO-OHpic exposure showed no cytotoxic effects in PDLSCs; however, at 5 μM, it markedly decreased colony-forming efficiency and impaired cell migration. Under osteogenic induction conditions, 5 μM VO-OHpic markedly attenuated mineralisation and downregulated the osteogenic marker gene expression partly through ERK signalling. Indeed, VO-OHpic impaired intracellular lipid accumulation during adipogenic differentiation, as evidenced by reduced expression of adipogenic marker genes. RNA sequencing analysis revealed that VO-OHpic treatment upregulated genes in the TGF-β and calcium signalling pathways, suggesting a regulatory role in PDLSC differentiation. In conclusion, PTEN regulates PDLSC colony formation, migration, and differentiation, suggesting a pivotal role for PTEN in maintaining periodontal tissue homeostasis. Full article
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